From Classical Radiation to Modern Radiation: Past, Present, and Future of Radiation Mutation Breeding

Radiation mutation breeding has been used for nearly 100 years and has successfully improved crops by increasing genetic variation. Global food production is facing a series of challenges, such as rapid population growth, environmental pollution and climate change. How to feed the world's enormous human population poses great challenges to breeders. Although advanced technologies, such as gene editing, have provided effective ways to breed varieties, by editing a single or multiple specific target genes, enhancing germplasm diversity through mutation is still indispensable in modern and classical radiation breeding because it is more likely to produce random mutations in the whole genome. In this short review, the current status of classical radiation, accelerated particle and space radiation mutation breeding is discussed, and the molecular mechanisms of radiation-induced mutation are demonstrated. This review also looks into the future development of radiation mutation breeding, hoping to deepen our understanding and provide new vitality for the further development of radiation mutation breeding.

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Short Review of Multichannel Membrane Capacitive Deionization: Principle, Current Status, and Future Prospect

Applied Sciences ◽

10.3390/app10020683 ◽

2020 ◽

Vol 10 (2) ◽

pp. 683 ◽

Cited By ~ 6

Author(s):

Nayeong Kim ◽

Jiho Lee ◽

Seonghwan Kim ◽

Sung Pil Hong ◽

Changha Lee ◽

...

Keyword(s):

Continuous Process ◽

Short Review ◽

Water Desalination ◽

Current Status ◽

Future Research ◽

Capacitive Deionization ◽

Ion Removal ◽

Removal Capacity ◽

Desalination Technology ◽

Further Development

Capacitive deionization (CDI) has gained a lot of attention as a promising water desalination technology. Among several CDI architectures, multichannel membrane CDI (MC-MCDI) has recently emerged as one of the most innovative systems to enhance the ion removal capacity. The principal feature of MC-MCDI is the independently controllable electrode channels, providing a favorable environment for the electrodes and enhancing the desalination performance. Furthermore, MC-MCDI has been studied in various operational modes, such as concentration gradient, reverse voltage discharging for semi-continuous process, and increase of mass transfer. Furthermore, the system configuration of MC-MCDI has been benchmarked for the extension of the operation voltage and sustainable desalination. Given the increasing interest in MC-MCDI, a comprehensive review is necessary to provide recent research efforts and prospects for further development of MC-MCDI. Therefore, this review actively addresses the major principle and operational features of MC-MCDI along with conventional CDI for a better understanding of the MC-MCDI system. In addition, the innovative applications of MC-MCDI and their notable improvements are also discussed. Finally, this review briefly mentions the major challenges of MC-MCDI as well as proposes future research directions for further development of MC-MCDI as scientific and industrial desalination technologies.

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Portable Life Support System: Current Status and Future Perspectives

Recent Patents on Engineering ◽

10.2174/1872212113666190625124429 ◽

2020 ◽

Vol 14 (1) ◽

pp. 12-28

Author(s):

Jingang Jiang ◽

Yihao Chen ◽

Xuefeng Ma ◽

Yongde Zhang ◽

Zhiyuan Huang ◽

...

Keyword(s):

Support System ◽

Life Support ◽

Support Systems ◽

Vital Signs ◽

Current Status ◽

Life Support System ◽

Life Support Systems ◽

Special Circumstances ◽

And Control ◽

Further Development

Background: Portable life support system is used in the battlefield, disaster and in other special circumstances such as in space exploration, and underground survey to give the wounded a life support. The most dangerous period for the injured is the first hour after an injury, which is a crucial time for treatment. If the patient's vital signs were stabilized, more than 40% of the injured could be saved. The staff can efficiently complete the task if they get effective and stable vital signs during the operation. Therefore, in order to reduce the risk of disaster and battlefield mortality to improve operational safety and efficiency, it is necessary to study the portable life support system. Objective: The study aimed to provide an overview of recent portable life support system and its characteristics and design. Methods: This paper introduces the patents and products related to a portable life support system, and its characteristics and application. Results: This paper summarizes five kinds of portable life support systems which are box type, stretcher type, bed type, backpack type and mobile type. Moreover, the characteristics of different portable life support systems are analyzed. The paper expounds the problems of different types of portable life support systems and puts forward improvement methods to solve the problems. Finally, the paper points out the future development of the system. Conclusion: Portable life support system plays an increasingly important role in health care. In terms of the structure, function and control, further development and improvements are needed, along with the research on portable life support system.

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Hydrodynamic slippage of water at surfaces

10.1093/oso/9780198789352.003.0004 ◽

2017 ◽

Author(s):

E. Charlaix ◽

L. Bocquet

Keyword(s):

Boundary Condition ◽

Molecular Mechanisms ◽

Slip Length ◽

Molecular Simulations ◽

Short Review ◽

Solid Surfaces ◽

Experimental Investigations

The boundary condition (B.C.) for hydrodynamic flows at solid surfaces is usually assumed to be that of no slip. However a number of molecular simulations and experimental investigations over the last two decades have demonstrated violations of the no-slip B.C., leading to hydrodynamic slippage at solid surfaces. In this short review, we explore the molecular mechanisms leading to hydrodynamic slippage of water at various surfaces and discuss experimental investigations allowing us to measure the so-called slip length

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The Integrated RNA Landscape of Renal Preconditioning against Ischemia-Reperfusion Injury

Journal of the American Society of Nephrology ◽

10.1681/asn.2019050534 ◽

2020 ◽

Vol 31 (4) ◽

pp. 716-730 ◽

Cited By ~ 1

Author(s):

Marc Johnsen ◽

Torsten Kubacki ◽

Assa Yeroslaviz ◽

Martin Richard Späth ◽

Jannis Mörsdorf ◽

...

Keyword(s):

Gene Expression ◽

Reperfusion Injury ◽

Caloric Restriction ◽

Molecular Mechanisms ◽

Target Genes ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Hypoxic Preconditioning ◽

Preventive Strategies ◽

Gene Expression Signatures

BackgroundAlthough AKI lacks effective therapeutic approaches, preventive strategies using preconditioning protocols, including caloric restriction and hypoxic preconditioning, have been shown to prevent injury in animal models. A better understanding of the molecular mechanisms that underlie the enhanced resistance to AKI conferred by such approaches is needed to facilitate clinical use. We hypothesized that these preconditioning strategies use similar pathways to augment cellular stress resistance.MethodsTo identify genes and pathways shared by caloric restriction and hypoxic preconditioning, we used RNA-sequencing transcriptome profiling to compare the transcriptional response with both modes of preconditioning in mice before and after renal ischemia-reperfusion injury.ResultsThe gene expression signatures induced by both preconditioning strategies involve distinct common genes and pathways that overlap significantly with the transcriptional changes observed after ischemia-reperfusion injury. These changes primarily affect oxidation-reduction processes and have a major effect on mitochondrial processes. We found that 16 of the genes differentially regulated by both modes of preconditioning were strongly correlated with clinical outcome; most of these genes had not previously been directly linked to AKI.ConclusionsThis comparative analysis of the gene expression signatures in preconditioning strategies shows overlapping patterns in caloric restriction and hypoxic preconditioning, pointing toward common molecular mechanisms. Our analysis identified a limited set of target genes not previously known to be associated with AKI; further study of their potential to provide the basis for novel preventive strategies is warranted. To allow for optimal interactive usability of the data by the kidney research community, we provide an online interface for user-defined interrogation of the gene expression datasets (http://shiny.cecad.uni-koeln.de:3838/IRaP/).

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Immunomodulatory Effects of Radiotherapy

International Journal of Molecular Sciences ◽

10.3390/ijms21218151 ◽

2020 ◽

Vol 21 (21) ◽

pp. 8151

Author(s):

Sharda Kumari ◽

Shibani Mukherjee ◽

Debapriya Sinha ◽

Salim Abdisalaam ◽

Sunil Krishnan ◽

...

Keyword(s):

Molecular Mechanisms ◽

Carbon Particle ◽

Dna Lesions ◽

X Rays ◽

Adaptive Immune ◽

High Let Radiation ◽

Different Types ◽

High Let ◽

Radiation Induced ◽

Tumor Death

Radiation therapy (RT), an integral component of curative treatment for many malignancies, can be administered via an increasing array of techniques. In this review, we summarize the properties and application of different types of RT, specifically, conventional therapy with x-rays, stereotactic body RT, and proton and carbon particle therapies. We highlight how low-linear energy transfer (LET) radiation induces simple DNA lesions that are efficiently repaired by cells, whereas high-LET radiation causes complex DNA lesions that are difficult to repair and that ultimately enhance cancer cell killing. Additionally, we discuss the immunogenicity of radiation-induced tumor death, elucidate the molecular mechanisms by which radiation mounts innate and adaptive immune responses and explore strategies by which we can increase the efficacy of these mechanisms. Understanding the mechanisms by which RT modulates immune signaling and the key players involved in modulating the RT-mediated immune response will help to improve therapeutic efficacy and to identify novel immunomodulatory drugs that will benefit cancer patients undergoing targeted RT.

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Clinical Candidates Targeting the ATR–CHK1–WEE1 Axis in Cancer

Cancers ◽

10.3390/cancers13040795 ◽

2021 ◽

Vol 13 (4) ◽

pp. 795

Author(s):

Lukas Gorecki ◽

Martin Andrs ◽

Jan Korabecny

Keyword(s):

Cell Cycle ◽

Cancer Cells ◽

Molecular Mechanisms ◽

Patient Survival ◽

Current Status ◽

Future Perspectives ◽

Phase Ii Trials ◽

Anticancer Treatment ◽

Positive Outlook ◽

Insight Into

Selective killing of cancer cells while sparing healthy ones is the principle of the perfect cancer treatment and the primary aim of many oncologists, molecular biologists, and medicinal chemists. To achieve this goal, it is crucial to understand the molecular mechanisms that distinguish cancer cells from healthy ones. Accordingly, several clinical candidates that use particular mutations in cell-cycle progressions have been developed to kill cancer cells. As the majority of cancer cells have defects in G1 control, targeting the subsequent intra‑S or G2/M checkpoints has also been extensively pursued. This review focuses on clinical candidates that target the kinases involved in intra‑S and G2/M checkpoints, namely, ATR, CHK1, and WEE1 inhibitors. It provides insight into their current status and future perspectives for anticancer treatment. Overall, even though CHK1 inhibitors are still far from clinical establishment, promising accomplishments with ATR and WEE1 inhibitors in phase II trials present a positive outlook for patient survival.

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Examination of the Transcriptional Response to LaMIR166a Overexpression in Larix kaempferi (Lamb.) Carr

Biology ◽

10.3390/biology10070576 ◽

2021 ◽

Vol 10 (7) ◽

pp. 576

Author(s):

Yanru Fan ◽

Wanfeng Li ◽

Zhexin Li ◽

Shaofei Dang ◽

Suying Han ◽

...

Keyword(s):

Cell Lines ◽

Molecular Mechanisms ◽

Target Genes ◽

De Novo ◽

Pearson Correlation ◽

Transcriptional Response ◽

Correlation Coefficients ◽

Embryonic Cell ◽

Larix Kaempferi ◽

Transgenic Cell

The study of somatic embryogenesis can provide insight into early plant development. We previously obtained LaMIR166a-overexpressing embryonic cell lines of Larix kaempferi (Lamb.) Carr. To further elucidate the molecular mechanisms associated with miR166 in this species, the transcriptional profiles of wild-type (WT) and three LaMIR166a-overexpressing transgenic cell lines were subjected to RNA sequencing using the Illumina NovaSeq 6000 system. In total, 203,256 unigenes were generated using Trinity de novo assembly, and 2467 differentially expressed genes were obtained by comparing transgenic and WT lines. In addition, we analyzed the cleaved degree of LaMIR166a target genes LaHDZ31–34 in different transgenic cell lines by detecting the expression pattern of LaHdZ31–34, and their cleaved degree in transgenic cell lines was higher than that in WT. The downstream genes of LaHDZ31–34 were identified using Pearson correlation coefficients. Yeast one-hybrid and dual-luciferase report assays revealed that the transcription factors LaHDZ31–34 could bind to the promoters of LaPAP, LaPP1, LaZFP5, and LaPHO1. This is the first report of gene expression changes caused by LaMIR166a overexpression in Japanese larch. These findings lay a foundation for future studies on the regulatory mechanism of miR166.

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Smartphone-Enabled Personalized Diagnostics: Current Status and Future Prospects

Diagnostics ◽

10.3390/diagnostics11061067 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1067

Author(s):

Karla Jaimes Merazzo ◽

Joseba Totoricaguena-Gorriño ◽

Eduardo Fernández-Martín ◽

F. Javier del Campo ◽

Eva Baldrich

Keyword(s):

Mobile Devices ◽

Point Of Care ◽

Near Field ◽

Short Review ◽

Magnetic Sensors ◽

Current Status ◽

Wireless Power ◽

Communication Devices ◽

New Applications ◽

The Internet Of Things

Smartphones are becoming increasingly versatile thanks to the wide variety of sensor and actuator systems packed in them. Mobile devices today go well beyond their original purpose as communication devices, and this enables important new applications, ranging from augmented reality to the Internet of Things. Personalized diagnostics is one of the areas where mobile devices can have the greatest impact. Hitherto, the camera and communication abilities of these devices have been barely exploited for point of care (POC) purposes. This short review covers the recent evolution of mobile devices in the area of POC diagnostics and puts forward some ideas that may facilitate the development of more advanced applications and devices in the area of personalized diagnostics. With this purpose, the potential exploitation of wireless power and actuation of sensors and biosensors using near field communication (NFC), the use of the screen as a light source for actuation and spectroscopic analysis, using the haptic module to enhance mass transport in micro volumes, and the use of magnetic sensors are discussed.

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Crocetin Mitigates Irradiation Injury in an In Vitro Model of the Pubertal Testis: Focus on Biological Effects and Molecular Mechanisms

Molecules ◽

10.3390/molecules26061676 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1676

Author(s):

Giulia Rossi ◽

Martina Placidi ◽

Chiara Castellini ◽

Francesco Rea ◽

Settimio D'Andrea ◽

...

Keyword(s):

In Vitro Model ◽

Molecular Mechanisms ◽

Biological Effects ◽

Cellular Damage ◽

X Rays ◽

Adolescent Cancer ◽

Radiation Induced ◽

Vitro Model ◽

Underlying Mechanisms

Infertility is a potential side effect of radiotherapy and significantly affects the quality of life for adolescent cancer survivors. Very few studies have addressed in pubertal models the mechanistic events that could be targeted to provide protection from gonadotoxicity and data on potential radioprotective treatments in this peculiar period of life are elusive. In this study, we utilized an in vitro model of the mouse pubertal testis to investigate the efficacy of crocetin to counteract ionizing radiation (IR)-induced injury and potential underlying mechanisms. Present experiments provide evidence that exposure of testis fragments from pubertal mice to 2 Gy X-rays induced extensive structural and cellular damage associated with overexpression of PARP1, PCNA, SOD2 and HuR and decreased levels of SIRT1 and catalase. A twenty-four hr exposure to 50 μM crocetin pre- and post-IR significantly reduced testis injury and modulated the response to DNA damage and oxidative stress. Nevertheless, crocetin treatment did not counteract the radiation-induced changes in the expression of SIRT1, p62 and LC3II. These results increase the knowledge of mechanisms underlying radiation damage in pubertal testis and establish the use of crocetin as a fertoprotective agent against IR deleterious effects in pubertal period.

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The role of m6A modification in the biological functions and diseases

Signal Transduction and Targeted Therapy ◽

10.1038/s41392-020-00450-x ◽

2021 ◽

Vol 6 (1) ◽

Author(s):

Xiulin Jiang ◽

Baiyang Liu ◽

Zhi Nie ◽

Lincan Duan ◽

Qiuxia Xiong ◽

...

Keyword(s):

Cancer Progression ◽

Molecular Mechanisms ◽

Target Genes ◽

Rna Modification ◽

Biological Functions ◽

Rna Methylation ◽

Downstream Target ◽

Different Types ◽

M6a Rna Methylation

AbstractN6-methyladenosine (m6A) is the most prevalent, abundant and conserved internal cotranscriptional modification in eukaryotic RNAs, especially within higher eukaryotic cells. m6A modification is modified by the m6A methyltransferases, or writers, such as METTL3/14/16, RBM15/15B, ZC3H3, VIRMA, CBLL1, WTAP, and KIAA1429, and, removed by the demethylases, or erasers, including FTO and ALKBH5. It is recognized by m6A-binding proteins YTHDF1/2/3, YTHDC1/2 IGF2BP1/2/3 and HNRNPA2B1, also known as “readers”. Recent studies have shown that m6A RNA modification plays essential role in both physiological and pathological conditions, especially in the initiation and progression of different types of human cancers. In this review, we discuss how m6A RNA methylation influences both the physiological and pathological progressions of hematopoietic, central nervous and reproductive systems. We will mainly focus on recent progress in identifying the biological functions and the underlying molecular mechanisms of m6A RNA methylation, its regulators and downstream target genes, during cancer progression in above systems. We propose that m6A RNA methylation process offer potential targets for cancer therapy in the future.

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