scholarly journals Prevalence and Antibiotic Resistance Profile of Bacterial Pathogens in Aerobic Vaginitis: A Retrospective Study in Italy

Antibiotics ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1133
Author(s):  
Enrica Serretiello ◽  
Biagio Santella ◽  
Veronica Folliero ◽  
Domenico Iervolino ◽  
Emanuela Santoro ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Resistance Pattern ◽  
Bacterial Strains ◽  
Aerobic Vaginitis ◽  
E Coli ◽  
Antibiotic Resistance Profile ◽  
Proper Diagnosis ◽  
Susceptibility Tests ◽  
Lactobacillus Spp ◽  
Infectious Condition

Aerobic vaginitis (AV) is a vaginal infectious condition, characterized by a high inflammatory response and/or signs of epithelial atrophy, a decrease in the amount of Lactobacillus spp. and an increase in enteric origin bacteria. AV, often misdiagnosed, is difficult to treat due to the emerging spread of multi-drug resistant bacterial strains. The present study aimed to define the prevalence of AV, to detect causative bacteria and their antimicrobial resistance pattern. Women 10–95 years old, admitted to San Giovanni di Dio e Ruggi d’Aragona Hospital, Salerno, Italy (in the years 2015–2019) are included in the study. Bacterial identification and antibiotic susceptibility tests were carried out by VITEK® 2. Among 2069 patients, 1176 tested positive for microbial growth. A higher incidence of infection was found in the 55–64 age group. Among the pathogenic strains, 50.4% were Gram-negative, and 49.6% were Gram-positive. Escherichia coli (E. coli) (32.5%) was the most representative strain, followed by Enterococcus faecalis (E. faecalis) (29.4%), Klebsiella pneumoniae (K. pneumoniae) (7.8%) and Enterococcus faecium (E. faecium) (7.7%). E. coli showed high sensitivity to carbapenems and amikacin. K. pneumoniae carbapenems resistance was fluctuating over time. Alarming resistance to vancomycin was not recorded for Enterococci. Both strains were sensitive to teicoplanin, linezolid and tigecycline. Proper diagnosis and an effective therapeutic approach are needed to improve AV management.

scholarly journals Microbial Isolates from Vegetable Foreign Bodies Inhaled by Dogs

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Sara Flisi ◽  
Manuel Dall’Aglio ◽  
Costanza Spadini ◽  
Clotilde Silvia Cabassi ◽  
Fausto Quintavalla
Keyword(s):  
Respiratory Tract ◽  
Antibiotic Susceptibility ◽  
Resistant Bacteria ◽  
Upper Respiratory Tract ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Bacteria And Fungi ◽  
Susceptibility Tests ◽  
The Right

Grass-seed inhalation is a common problem in canine patients, in particular during summer months, migrating in upper and lower respiratory tract. Grass awns can harbor bacteria and fungi, causing grass seeds foreign body-related disease (GSFBD). Aim of this study was to investigate the aerobic microbial flora isolated from grass awns extracted from 41 dogs with GSFBD and the antibiotic susceptibility of the isolated bacterial strains. Fifty-four grass awns were localized with diagnostic imaging tests and removed by endoscopy from respiratory tract. The most frequent localizations were in the left nostril and the right hemithorax. Only one grass awn was extracted from each patient except in 7 that had more than one. Bacteriological and mycological cultures, strains identification, and antibiotic susceptibility tests were performed. One or more bacterial strains were isolated from all grass awns. Fungal strains were isolated only in 4 cases. Staphylococcussp. was the most frequent isolate in the upper respiratory tract (36.8%), whileE. coli(24.4%) was the most frequent isolate in the lower tract. Fluoroquinolones and Doxycycline were the most effective antibiotics, while resistance was observed against Gentamicin (>93%), Cefapirin, and Clindamycin (>80%). These data are relevant in relation to the use of these antibiotics in both animals and humans, for the risk of transmission of antibiotic resistant bacteria or resistance genes.

scholarly journals ANTIMICROBIAL ACTIVITY OF METHANOLIC EXTRACT OF LUDWIGIA PARVIFLORA L. AGAINST STANDARD BACTERIAL STRAINS AND COMPARISON OF ITS ACTIVITY WITH THAT OF STANDARD ANTIBIOTICS

2019 ◽  
pp. 430-432
Author(s):  
SHIBU GEORGE ◽  
MEVLIN JOY
Keyword(s):  
Antimicrobial Activity ◽  
Bacterial Infections ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Whole Plant ◽  
The Common ◽  
Susceptibility Tests

Objective: The objective of this study was to evaluate the antimicrobial activity of methanolic extract of Ludwigia parviflora L. using standard bacterial strains and compare its activity with that of standard antibiotics. Methods: The antibacterial activity and antibiotic susceptibility tests were done by disk diffusion method using MTCC bacterial strains. Results: The study revealed that the methanolic extract of the whole plant of L. parviflora L. was effective to inhibit the growth of Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli. Among the tested strains, S. aureus, P. aeruginosa, K. pneumoniae, and E. coli were more susceptible to the methanolic extract of L. parviflora than the commonly using antibiotic tetracycline 30 mcg. The activity of methanolic extract was also higher than the activity of gentamicin 10 mcg against the P. aeruginosa. Conclusion: The study concluded that the crude methanolic extract of the whole plant of L. parviflora L. is a good source for antibacterial agent against S. aureus, P. aeruginosa, K. pneumoniae, and E. coli. Hence, this plant can be used as a natural alternative to the common antibiotics such as gentamicin and tetracycline against common bacterial infections after validating its pharmacological and toxicological activities.

scholarly journals Antibacterial Activity of the Original Dietary Supplement Oxidal® in Vitro

2020 ◽  
Vol 11 ◽  
pp. 71-78
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Dietary Supplement ◽  
Antibacterial Effect ◽  
High Sensitivity ◽  
Bacterial Strains ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
The Mean ◽  
The One

Studies have been conducted to determine the antibacterial effect of Oxidal® and anolyte against Staphylococcus aureus and Escherichia coli. The bacterial strains tested showed high sensitivity to the dietary supplement Oxidal® and to anolyte, as well as to the control antibiotic Thiamphenicol. The mean MPC50 values of Oxidal® for Gram-positive bacteria S. aureus (0.21 ± 0.13 mg/ml) were lower than those for Gram-negative E. coli (0.55 ± 0.24 mg/ml). The dose that fully suppressed S. aureus growth was 0.86 ± 0.52 mg/ml, while for E. coli this dose was significantly higher - 2.40 ± 0.80 mg/ml (P<0.05). For the antibiotic Thiamphenicol, the results were opposite - the mean MPC50 values for E. coli (2.40 ± 0.80) were lower than those for S. aureus (3.71 ± 2.86). Under the influence of 10% Oxidal® solution, the amount of viable S. aureus and E. coli decreased by more than 30% over the untreated control after 20 min. After 45 minutes, only 37% of S. aureus cells and 19% of E. coli cells developed upon cultivation, and after 90 minutes of exposure, about 20% of the cells of the microorganisms understudy of both species remained viable. The fresh anolyte and the one stored for 1 year inactivated S. aureus and E. coli for 2 min in a suspension at a density of 106 cells/ml.

scholarly journals Study of the in vitro Anti-bacterial Susceptibility of the Commercially Available Antibiotics in Suspension or Solution Forms Collected within Dhaka Metropolis

2020 ◽  
Vol 36 (2) ◽  
pp. 79-83
Author(s):  
Asma Akter Bhuiyan ◽  
Mehran Mosharraff Hossain Niloy ◽  
Anamika Chakrabarty ◽  
Syeda Muntaka Maniha ◽  
Rashed Noor
Keyword(s):  
Bacterial Infections ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
E Coli ◽  
Antibiotic Solution ◽  
Test Organisms ◽  
Antibacterial Susceptibility ◽  
Susceptibility Tests ◽  
The City

Consumption of a range of antibiotics upon bacterial infections is a common chemotherapeutic practice. Current study attempted to detect the in vitro anti-bacterial activities of such finished medicaments against some of the selected bacterial strains in order to examine their sustainable effectiveness against microorganisms. A total of six categories of samples were randomly collected from different pharmacies within the city of Dhaka. The antibacterial susceptibility tests were conducted employing the Kirby-Bauer (agar well diffusion) method. The test bacterial strains used in this study were Escherichia coli, Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and P. putida and P. fluorescence. While E. coli was found to be sensitive towards all the antibiotics used, others showed resistance to a significant extent. flucloxacillin, cephradine and salbutamol were noticed to be completely inactive against Pseudomonas species. Besides, the salbutamol was found only to suppress the growth of E. coli but the other five test organisms were completely resistant against this antibiotic solution. Bangladesh J Microbiol, Volume 36 Number 2 December 2019, pp 79-83

scholarly journals Antimicrobial resistance patterns of commensal Escherichia coli isolated from feces of non-diarrheic dogs in Grenada, West Indies

2019 ◽  
Vol 12 (12) ◽  
pp. 2070-2075 ◽  
Author(s):  
Victor A. Amadi ◽  
Harry Hariharan ◽  
Ozioma A. Amadi ◽  
Vanessa Matthew-Belmar ◽  
Roxanne Nicholas-Thomas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Resistance Rate ◽  
Resistance Pattern ◽  
E Coli ◽  
Resistance Patterns ◽  
Antimicrobial Susceptibility Tests ◽  
Susceptibility Tests ◽  
Selection Of

Background and Aim: There is currently no published information on the prevalence and antimicrobial susceptibility patterns of commensal Escherichia coli in dogs of Grenada origin. Monitoring antimicrobial resistance helps in the empirical selection of antibiotics. This study determined the occurrence of E. coli including the O157:H7 serotype in feces of non-diarrheic dogs of Grenada origin and the antibiotic resistance pattern of the E. coli isolates. Materials and Methods: Fecal samples from 142 of the 144 (98.6%) dogs were culture positive for E. coli. Selection of up to three colonies from each of the 142 E. coli-positive samples yielded a total of 402 E. coli isolates, which were analyzed for the presence of non-sorbitol fermenting colonies, and O157-agglutination. Results: Of the 402 E. coli isolates, 30 (7.5%) were non-sorbitol fermenters. However, none of the 402 isolates gave a positive reaction (O157:H7) to the E. coli O157:H7 latex kit. Antimicrobial susceptibility tests against 12 antibiotics revealed low resistance rates to all the tested antibiotics except for tetracycline (Te) (23.4%), cephalothin (CF) (13.2%), and ampicillin (AM) (7.7%). Thirty-nine out of the 402 (9.7%), E. coli isolates were resistant to two or more antibiotics of different classes. Conclusion: This is the first report of isolation and antimicrobial susceptibilities of commensal E. coli from non-diarrheic dogs in Grenada. Some of the isolates (39/402 isolates, 9.7%) were resistant to multiple antibiotics. This study showed that presently, dogs in Grenada should not be considered a reservoir for the E. coli O157:H7 serotype and for multiple antibiotic-resistant E. coli strains. Among the 402 E. coli isolates, the resistance rate to drugs other than Te, CF, and AM was very low.

scholarly journals Antibiotic Resistance of Bacteria Isolated from Water Supplies Used in Poultry Production in Ashanti Region of Ghana

2021 ◽  
Vol 15 (1) ◽  
pp. 7-15
Author(s):  
Frank B. Osei ◽  
Vivian E. Boamah ◽  
Yaw D. Boakye ◽  
Christian Agyare ◽  
Robert C. Abaidoo
Keyword(s):  
Culture Media ◽  
Resistance Pattern ◽  
Poultry Production ◽  
Drug Resistant ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
E Coli ◽  
Ashanti Region ◽  
Quality Of Water

Objective: Water plays an important role in both domestic and commercial settings. Pathogenic microbial contaminants, however, render water unsafe for use. There are several reports on the quality of water used for drinking purposes in humans but few studies have been conducted on the microbial quality of water used in animal farming. Methods: In this study, the resistance pattern of bacterial isolates from drinking water used in poultry production in the Ashanti region of Ghana from our previously published report was determined. The presence of Escherichia coli, Salmonella typhi, Staphylococcus aureus and coagulase-negative Staphylococci was determined using selective culture media (pour plate method) and confirmed through Gram staining and biochemical reactions. Antibiotic sensitivity of isolates was determined followed by detection of Extended-Spectrum Beta-Lactamase (ESBL) producing Gram-negative isolates. Results: The study revealed that water used in poultry farms contains sources of multi-drug resistant strains of E. coli, S. typhi, S. aureus and coagulase-negative staphylococci. E. coli, S. typhi, S. aureus and coagulase-negative staphylococci were recovered from 31%, 36%, 64% and 19% of samples, respectively. Majority of these isolates were resistant to cephalosporins and penicillins. Almost 95% of the bacterial isolates were multi-drug resistant. None of E. coli and S. typhi isolates produced ESBL. Conclusion: There is a need for stringent regulations and stringent measures should be taken to make these various sources of water safe for use in animal husbandry as these waters are a potential source of pathogenic and resistant bacterial strains which can cause infections to the animals and farm workers.

scholarly journals Antibiotic Sensitivity Profiles of Salmonella typhimurium and E. coli O157: H7 Isolates from Ready to Eat Chicken Meat in Ibadan-Nigeria

2019 ◽  
pp. 1-17
Author(s):  
Oluwatoyin Hephzibah Ajulo ◽  
Olusola Victoria Adetunji ◽  
Olugbenga Matthew Ajulo
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Typhimurium ◽  
Antibiotic Sensitivity ◽  
Chicken Meat ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
E Coli ◽  
Antibiotic Resistance Profile ◽  
Ibadan Nigeria

Background: The global increase in the use of antibiotics in poultry and livestock production has significantly resulted in the emergence of antibiotic resistant bacteria. There is a growing global concern of the effect of antibiotic resistance on both animals and humans. The aim of this study was to determine the antibiotic resistance profile of Salmonella typhimurium and E. coli 0157:H7 in ready to eat chicken meat in Ibadan, Nigeria. Method: A set of 500 pieces of chicken parts were purchased from sampled eateries in Ibadan, Nigeria. Standard microbiological techniques were used to isolate Salmonella and E. coli biochemically and serologically. All confirmed isolates were subjected to in vitro antibiotic susceptibility testing against 8 antibiotics of different classes of Gram negative antimicrobial-impregnated multidisks (ABTEK) Result: The resistance pattern revealed E. coli 0157:H7 and Salmonella isolates were resistant to 5 drugs namely; Ceftazidime, Cefuroxime, Augmentin, Nitrofurantoin and Ampicillin. Highest resistance of E. coli isolates to Augmentin and Ampicillin were indicated both having a frequency of 11 (84.6%) and least resistance to Gentamicin, Ciprofloxacin and Ofloxacin 0 (0.00%) were indicated. Three antibiotics that showed 100% sensitivity by all the isolates were Gentamicin, Ciprofloxacin and Ofloxacin. Conclusion: The study revealed that Isolated E. coli and Salmonella strains from ready to eat chicken parts were resistant to five antibiotics; Ceftazidime, Cefuroxime, Augmentin, Nitrofurantoin and Ampicillin and very susceptible to three antibiotics namely; Gentamicin, Ciprofloxacin and Ofloxacin.

SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF 3-FORMYL INDOLE BASED SCHIFF BASES

2018 ◽  
Vol 7 (6) ◽  
Author(s):  
Singh Gurvinder ◽  
Singh Prabhsimran ◽  
Dhawan R. K.
Keyword(s):  
Antimicrobial Activity ◽  
Spectral Analysis ◽  
Schiff Bases ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Fungal Strain ◽  
Bacterial Strains ◽  
Standard Drug ◽  
Gram Negative ◽  
E Coli

In order to develop new antimicrobial agents, a series of 3-formyl indole based Schiff bases were synthesized by reacting 3-formyl indole(indole-3-carboxaldehyde) with substituted aniline taking ethanol as solvent. The reaction was carried in the presence of small amount of p-toluene sulphonic acid as catalyst.All the synthesized compounds were characterized by IR, 1H-NMR spectral analysis. All the synthesized compounds were evaluated for antimicrobial activity against two gram positive bacterial strains (B. subtilisand S. aureus) and two gram negative bacterial strains (P. aeruginosaand E. coli) and one fungal strain (C. albicans). All the synthesized compounds were found to have moderate to good antimicrobial activity. The  standard drug amoxicillin, fluconazole were used for antimicrobial activity. Among the synthesized compounds, the maximum antimicrobial activity was shown by compounds GS04, GS07, GS08 and GS10.

Analysis of the monitoring study of the antibiotic-resistance of the agents of purulent-inflammatory processes of soft tissue

2018 ◽  
Vol 22 (2) ◽  
pp. 285-288
Author(s):  
A.P. Prevar ◽  
A.V. Kryzshanovskaya ◽  
V.A. Radionov ◽  
V.M. Mrug
Keyword(s):  
Soft Tissues ◽  
High Sensitivity ◽  
Biochemical Properties ◽  
Antimicrobial Drugs ◽  
Strict Adherence ◽  
E Coli ◽  
Monitoring Study ◽  
Treatment Measures ◽  
Inflammatory Processes ◽  
Main Factor

The main factor in the treatment of suppurative and inflammatory processes is the timely optimization of treatment measures taking into account the nature of the microflora and its susceptibility to antimicrobial drugs. The purpose of the study is to monitor the spectrum of microorganisms – pathogens of purulent-inflammatory processes of soft tissues in surgical patients; study of the sensitivity of isolated strains to antibiotics. The material was collected in accordance with aseptic rules. The identification of a pure culture of bacteria was carried out according to morphological, culture, biochemical properties, and the presence of virulence enzymes. Sensitivity of bacteria to antibiotics was determined by the standard disks method (by Kirby-Bauer’s). 255 patients with purulent-inflammatory processes of soft tissues were examined for the period from 2014 to 2017. 229 strains of isolated bacteria were included to Escherichia coli, Citrobacer freundii, Enterobacter cloacae, E.aerogenes, Proteus vulgaris, Staphylococcus aureus, S.epidermidis, Streptococcus pyogenes, S.viridians, S.agalactiae, Pseudomonas aeruginosa. The main cause of purulent-inflammatory processes of soft tissues is Staphylococci (67,2%). Compared to previous studies, the number of P.aeruginosa isolated cultures increased (7.9%). In monoculture and in association with other microorganisms, E. coli (9.6% of cases), E.cloacae et aerogenes (3.9% of cases), P.vulgaris (3.9% of cases), C.freundi (2.5% of cases), S.agalactiae, S.pyogenes, S.viridans (3.5%). The number of associated sows reaches 12%. Clinical strains of microorganisms remain most sensitive to fluoroquinolones, cephalosporins, and also retains high sensitivity to gentamicin, lincomycin, rifampicin, which is important for empirical antibiotic therapy. To increase the effectiveness of antibacterial therapy, strict adherence to the mode of appointment of antibiotics, justification of indications, a combination of antibiotics of different spectrum of action, mandatory correction after determining the sensitivity of the pathogen.

The Consumption of Galactomannan Effervescent Drinks made from Coconut Pulp Waste and Colonic Microbiota in Wistar Rats

2020 ◽  
Vol 15 (1) ◽  
pp. 52-56
Author(s):  
Sri Winarti ◽  
Agung Pasetyo
Keyword(s):  
Fatty Acids ◽  
Gut Microbiota ◽  
Wistar Rats ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
E Coli ◽  
Colonic Microbiota ◽  
Male Wistar Rats ◽  
Lactobacillus Spp ◽  
Chain Fatty Acids

The consumption of prebiotics is known to affect the balance of gut microbiota. The purpose of this study was to explore how a galactomannan-rich effervescent drink can affect the population of Lactobacillus, Bifidobacterium, E. coli, and the concentration of short-chain fatty acids in the cecum of rats. Twenty-eight male Wistar rats (aged 2 months) were divided equally into 7 groups and treated orally each day for 15 days with 2 mL effervescent drinks with increasing levels of prebiotic galactomannan. The dosage of 500 mg galactomannan increased the growth of Lactobacillus spp. and Bifidobacterium spp. with inhibition of the growth of E.coli with increased formation of short-chain fatty acids such as acetate, propionate, and butyrate in the cecum of rats.

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