scholarly journals Antioxidant Status of Broiler Chickens Fed Diets Supplemented with Vinification by-Products: A Valorization Approach

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1250
Author(s):  
Alexandros Mavrommatis ◽  
Elisavet Giamouri ◽  
Eleni D. Myrtsi ◽  
Epameinondas Evergetis ◽  
Katiana Filippi ◽  
...  

Vinification by-products display great potential for utilization as feed additives rich in antioxidant compounds. Thus, the effect of dietary ground grape pomace (GGP), wine lees extract rich in yeast cell walls (WYC), and grape stem extracts (PE) on the relative expression of several genes involved in liver oxidative mechanisms and the oxidative status of the blood and breast muscle of broiler chickens was investigated. In total, 240 one-day-old as hatched chicks (Ross 308) were assigned to four treatments, with four replicate pens and 15 birds in each pen. Birds were fed either a basal diet (CON) or a basal diet supplemented with 25 g/kg GGP, or 2 g/kg WYC, or 1 g starch including 100 mg pure stem extract/kg (PE) for 42 d. The polyphenolic content of vinification by-products was determined using an LC-MS/MS library indicating as prevailing compounds procyanidin B1 and B2, gallic acid, caftaric acid, (+)-catechin, quercetin, and trans-resveratrol. Body weight and feed consumption were not significantly affected. The relative transcript level of GPX1 and SOD1 tended to increase in the liver of WYC-fed broilers, while NOX2 tended to decrease in the PE group. SOD activity in blood plasma was significantly increased in WYC and PE compared to the CON group. The total antioxidant capacity measured with FRAP assay showed significantly higher values in the breast muscle of PE-fed broilers, while the malondialdehyde concentration was significantly decreased in both WYC- and PE-fed broilers compared to the CON group. The exploitation of vinification by-products as feed additives appears to be a promising strategy to improve waste valorization and supply animals with bioactive molecules capable of improving animals’ oxidative status and products’ oxidative stability.

2021 ◽  
Vol 13 (7) ◽  
pp. 4005
Author(s):  
Nikola Puvača ◽  
Snežana Tanasković ◽  
Vojislava Bursić ◽  
Aleksandra Petrović ◽  
Jordan Merkuri ◽  
...  

The aim of this research was the visual characterization and investigating the effects of Alternaria spp. contaminated wheat grains in the starter stage of broilers nutrition on productive parameters and oxidative stress. The research was divided into two phases. Bunches of wheat in post-harvest period of year 2020 was collected from a various locality in Serbia and Albania. In the first phase, collected samples were visual characterized by Alternaria spp. presence by color measurement methods. Gained results are conferred in the range of the color properties of grain color properties of Alternaria toxins. Wheat grain samples were significantly different (p < 0.05) in terms of all measured color parameters (L*, a*, b*). Classification of field fungi in analyzed wheat grain samples showed that the significant field fungi were Rhizopus spp., followed by Alternaria spp., and Fusarium spp. In the second phase, biological tests with chickens were carried out during the broiler chickens’ dietary starter period in the first 14th days of age. At the beginning of the experiment, a total of 180-day-old Ross 308 strain broilers were equally distributed into three dietary treatments, with four replicates each. Dietary treatments in the experiments were as follows: basal diet without visual contamination of Alternaria spp. with 25% wheat (A1), a basal diet with visual contamination of Alternaria spp. with 25% wheat from Serbia (A2), basal diet with visual contamination of Alternaria spp. with 25% wheat from Albania (A3). The trial with chickens lasted for 14 days. After the first experimental week, wheat infected with Alternaria spp. in treatment A2 and A3 expressed adverse effects. The highest body weight of chickens of 140.40 g was recorded in broilers on control treatment A1 with statistically significant differences (p < 0.05) compared to treatments A2 (137.32 g) and A3 (135.35 g). At the end of the second week of test period, a statistically significant (p < 0.05) difference in body weight of broiler chickens could be noticed. The highest body weight of 352.68 g was recorded in control treatment A1, with statistically significant differences compared to other Alternaria spp. treatments. The lowest body weight of chickens was recorded in treatment A3 (335.93 g). Results of feed consumption and feed conversion ratio showed some numerical differences between treatments but without any statistically significant differences (p > 0.05). Alternaria spp. contaminated diet increased glutathione (GSH), glutathione reductase (GR), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) and decreased peroxidase (POD) and superoxide dismutase (SOD) serum levels, respectively. Built on the achieved results, it can be concluded that the wheat contaminated with Alternaria spp. in broilers nutrition negatively affected growth, decreased oxidative protection and interrupted chicken welfare in the first period of life.


2017 ◽  
Vol 41 (3) ◽  
pp. 243 ◽  
Author(s):  
Apriliana Devi Anggraini ◽  
Ferry Poernama ◽  
Chusnul Hanim ◽  
Nanung Danar Dono

This study was aimed to determine the effects of protease supplementation in diets with agricultural-livestock by products on the growth performance in broiler chickens. The commercial protease used in current study was produced by Bacillus licheniformis which based on keratinase. The treatment diets were a control basal diet without agricultural-livestock by-products and protease supplementation (P1); P1 + 0.05% protease; basal diet with meat bone meal (MBM) and distiller’s dried grain with soluble (DDGS) suppementation (P3); P3 + 0.05% protease (P4); basal diet with hydrolized chicken feather meal (HCFM) and DDGS supplementation (P5); P5 + 0.05% protease. The obtained data were statistically analyzed using Complete Randomized Design, and analyzed subsequently by Orthogonal Contrats Test. Results showed that protease supplementation in the diets containing agricultural-livestock by-products did not affect growth performance of broiler chickens in starter phase and overall age phase. However, in diets containing MBM, protease supplementation reduced the nett gain and feed conversion ratio (FCR) in finisher phase (P<0.05). In diets containing HCFM, protease supplementation increased nett gain and FCR (P<0.05), compared to those of containing MBM. It could be concluded that qualified HCFM, MBM, and DDGS can be used as alternatives for protein-energy source feed stuffs in starter phase, as they did not reduce growth performance of broiler chickens. Protease used in this study might be more effective in diets containing HCFM than those of containing MBM and DDGS.Keywords: Agricultural-livestock by-products, Broilers chickens, Growth performance, Protease.


Animals ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 162 ◽  
Author(s):  
Majid Shakeri ◽  
Jeremy Cottrell ◽  
Stuart Wilkinson ◽  
Mitchell Ringuet ◽  
John Furness ◽  
...  

Heat stress (HS) is an environmental stressor challenging poultry production and requires a strategy to cope with it. A total of 288-day-old male broiler chicks were fed with one of the following diets: basal diet, basal with betaine (BET), or with selenium and vitamin E (AOX), or with a combination of BET and AOX, under thermoneutral and cyclic HS. Results showed that HS reduced average daily feed intake (ADFI) (p = 0.01) and average daily gain (ADG) (p < 0.001), and impaired feed conversion ratio (FCR) (p = 0.03) during rearing period (0–42 day). BET increased ADG (p = 0.001) and decreased FCR (p = 0.02), whereas AOX had no effects. Breast muscle weight was decreased by HS (p < 0.001) and increased by BET (p < 0.001). Rectal temperature was increased by HS (p < 0.001) and improved by BET overall. Respiration rate was increased by HS (p < 0.001), but BET decreased it during HS (p = 0.04). Jejunum transepithelial resistance was reduced by HS and had no effect on permeability whereas BET increased jejunum permeability (p = 0.013). Overall, the reductions in ADG of broiler chickens during HS were ameliorated by supplementation with BET, with much of the increase in ADG being breast muscle.


Author(s):  
Mozer Manneti de ÁVILA ◽  
Mariane Castro MAYENS ◽  
Jéssica HALFEN ◽  
Antônio Amaral BARBOSA ◽  
Rutiele SILVEIRA ◽  
...  

ABSTRACT The aim of the present study was to evaluate the ingestive behavior of sheep receiving alternative food with grape pomace to replace roughage, as well as its impacts on the rumen environment. It was observed that BU had higher TOC, TRAM and pH of the ruminal liquid, while there was less TRU (P < 0,05). As for the other parameters, no significant differences were found. In this study, just as the feeding time was not influenced due to the similarity of the concentrate: roughage ratio in the diet, the protozoan count did not change. This finding reinforces the possibility of using grape marc as a tool for formulating feed, reducing the risk of ruminal disturbances. It was observed that the group control had lower TRAM, indicating that the diet of this group promoted greater microbial activity and, consequently, greater digestibility, corroborating the already observed TOC. This result was the opposite of what was expected, since the grape pomace has probiotic properties, precisely because the fruits have more fungi and bacteria in their microbiota. This also confirms that the co-products of vitiviniculture can act as promoters of the intestinal health of ruminants, justifying further studies in order to improve this use. Therefore, the use of wine by-products is an alternative to reduce production costs, as its use in diets for confined sheep can improve the performance of the animals, increasing the total feed consumption and improving microbial activity.


2020 ◽  
Vol 60 (16) ◽  
pp. 1902
Author(s):  
Esmail Mohammadi ◽  
Hossein Janmohammadi ◽  
Majid Olyayee ◽  
Javad Ashrafi Helan ◽  
Somayeh Kalanaky

Context Selenium (Se) is an essential trace element and plays pivotal roles in poultry nutrition. Aims The present study was designed to compare the impact of dietary supplementation of different sources of Se on growth performance, Se concentration of breast meat, and immune response of broiler chickens. Methods In total, 300 1-day-old as hatched broilers were randomly allocated to six dietary treatments, with five replicates of 10 birds per each. The experimental diets were as follows: (1) corn–soybean meal-based diet supplemented with 0.30 mg/kg of sodium selenite, (2) basal diet supplemented with 0.30 mg/kg of Se-enriched yeast, and (3–6) basal diet supplemented with 0.15, 0.30, 0.90 and 1.50 mg/kg of nano-Se respectively. Humoral immunity was assessed by antibody titer against a 5% sheep red blood-cell suspension and cellular immunity was measured by administration of phytohaemagglutinin-p at 38 days. Key results Results indicated that during the periods from 1 to 10 days, from 11 to 24 days, from 25 to 42 days and from 1 to 42 days, average daily gain, average daily feed intake, and feed conversion ratio did not differ among the treatments (P &gt; 0.05). Assessment of orthogonal contrasts at the whole phase of feeding showed that the average daily gain in the broilers fed organic Se was significantly (P &lt; 0.05) higher than that in those fed inorganic Se, as well as nano-Se in comparison to organic Se; such effects were not observed in earlier feeding stages. Se supplementation significantly increased the Se concentration of breast muscle (P &lt; 0.0001). The birds that received 1.50 mg/kg of diet nano-Se showed higher (P &lt; 0.05) total immunoglobulin and IgG titers in primary and secondary immune responses against sheep erythrocytes respectively. Toe web swelling after 24 h and 48 h of receiving phytohaemagglutinin-p was not affected by Se supplementation (P &gt; 0.05). Conclusions In conclusion, the addition of nano-Se gave better results than did inorganic (sodium selenite) and organic (yeast selenium) forms of Se in performance traits, breast meat Se concentration and antibody response of broilers. Implications Novel elemental nanometer particulates, including nano-Se, exhibit new characteristics and a different mode of actions in comparison with organic and inorganic forms of Se sources in poultry diets. Inclusion of the nano form of Se in broiler diets and study of productive performance, immunity responses and meat quality leads to improve balanced broiler diets formulation in view of Se.


Author(s):  
A. I. Petenko ◽  
E. S. Volobueva

When developing functional feed additives it is of interest to use microorganisms that have the ability to take root in the gastrointestinal tract of poultry and normalize its microflora to have a positive effect on the immune system. One of these bacteria is Propionibacterium shermanii, which has a fortifying, immunostimulating, antimutagenic effect, and can reduce the toxic effect of ultraviolet light and chemical compounds. Azotobacter vinelandii, which has probiotic fortifying, antibiotic properties, is effective as a stimulator of the development of the entire microbiota in a contaminated object, and recycles the formed ammonia in bird droppings, was chosen as another microorganism for creating the additive. The purpose of the research was to develop a functional biological additive based on probiotic microflora, using the by-products of crop processing in the formulations, and to select the norms for its introduction into the diet of quails. As a result of the research the technology of joint cultivation of Propionibacterium shermanii and Azotobacter vinelandii strains was developed, a universal starter culture was created that allows to ferment by-products of processing of plant raw materials, a functional additive with immunostimulating, vitamin and provitamin properties for quails has been obtained. The results of the production test confirmed the effectiveness of the developed additive in the diet of quails. Economic efficiency in the application of the proposed functional biological additive has been shown a reduction in feed consumption per 1 kg of live weight gain by 5,13 %. In addition, the economic effect of using the functional biological additive increases. Thus, the profit in the experimental group was 2738,34 rubles, which was 12,45 % higher than in the control group.


Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 364
Author(s):  
Emrah Gungor ◽  
Aydin Altop ◽  
Guray Erener

The effects of raw (GP) and fermented grape pomace (FGP) on the growth performance, some carcass parameters, antioxidant status, intestinal morphology, and selected bacterial species in broiler chicken were investigated in this study. Grape pomace was fermented with Aspergillus niger for 7 d. In total, 140 one-day-old male chicks (Ross 308) were randomly assigned to four treatment groups, with five replicates and seven birds each. Chickens were fed either a basal diet (CON) or the basal diet supplemented with 0.25 g/kg synthetic antioxidants (5% butylated hydroxytoluene, 1% butylated hydroxyanisole, and 11% ethoxyquin) (AO), or 15 g/kg GP (GP), or 15 g/kg FGP (FGP) for 42 d. Dietary GP raised serum glutathione peroxidase (p = 0.031) and superoxide dismutase (p = 0.021) levels, increased ileum lamina muscularis thickness (p = 0.016), and did not affect selected bacterial species in the cecum of broiler chickens. Dietary FGP improved body weight (p = 0.003), increased the serum catalase level (p = 0.032), and decreased the cecal Clostridium perfringens count (p = 0.033) but did not affect the ileal morphology of broiler chickens. The carcass parameters, malondialdehyde level, pH, and color of the breast meat of chickens were not changed by either GP or FGP supplementation. Chickens fed with the synthetic antioxidants had similar growth performance with the chickens fed with FGP but had better body weight (p = 0.003) and feed conversion ratio (p = 0.045) compared with the chickens fed with GP. The obtained results showed that FGP can be used as an alternative to synthetic antioxidants in broiler diets.


Author(s):  
Dalila Fadhila Hidayat ◽  
Agus Widodo ◽  
Diyantoro Diyantoro ◽  
M. Gandul Atik Yuliani

Backgrounds: Broiler chickens are high productivity livestock. To achieve this productivity, many farmers provide feed additives. Fermented milk is one of the feed additives given through drinking water. Providing feed additives, regularly, will affect the performance of these broilers. Purpose: To investigate the potential of providing fermented milk through drinking water on the performance of broiler chickens. Method: Direct observations and perform calculations on several aspects that support the research. Result: After making observations, it was known that there are differences in the starter phase and the finisher phase. The observation was carried out from the age of 0-27 days. Broiler performance determined by the parameters of feed consumption, chicken body weight, and feed conversion. Conclusion: The observation shows an increase in feed consumption. However, in feed conversion and body weight gain instability is caused by physical conditions, temperature, and palatability in each phase of the broiler chickens.


2019 ◽  
Vol 2 (1) ◽  
pp. 73-78
Author(s):  
Olusegun S. Adedeji ◽  
Shola R. Amao ◽  
Kayode S. Olugbemiga

Introduction: Various types of feed additives have been evaluated under commercial conditions and in experimental trials with the aim to achieve improvements on growth performance and the best economic return. Herbs, including Sesame indicum leaves, spices and various plant extracts/essential oils can be used as alternatives to replace antibiotic growth promoters as they are rich in phytochemicals (active compounds) that can be used to stimulate growth and health of the animals. The uses of all these herbs should be studied in relation to the blood and serum indices of the animals in order to determine their harmful level of consuming them by the animals. Therefore, the aim of the study is to determine the effect of different dietary inclusion levels of Sesame indicum leaves on haematology and serum parameters of broiler chickens at both starter and finisher phases. Methods: A total of one hundred and fifty 1-day old, unsexed (Marshal) broiler chicks were randomly divided into five experimental groups at rate of 30 birds per group. Each group was further subdivided into three replicates at the rate of ten chicks per replicate in a Complete Randomized Design. A maize-soybean basal diet served as control while the Sesame indicum leaves was added to the basal diets at level (0g, 10g, 20g, 30g and 40g/kg) resulting in five formulae, respectively. At the end of the 4th and 8th weeks respectively, six birds were randomly selected from each of the replicate and blood samples were collected from the jugular vein of birds for haematology and serum. Results: The results indicated that all the haematology and serum parameters were significantly (p < 0.05) affected by the different inclusion levels of S. indicum leaves but the value obtained were within the recommended range while birds fed with the diet containing 40g/kg of S. indicum leaves had the highest values. Conclusion: Inclusion of S. indicum leaves in the diets appear not to have detrimental effect on the haematology and serum parameters of the experimental birds. The study recommended that 40g/kg inclusion level be adopted as it improved growth performance and general well-being of broiler chickens.


2020 ◽  
Vol 47 (3) ◽  
pp. 234-244
Author(s):  
A. O. Fafiolu ◽  
J. O. Alabi ◽  
C. G. Godwin ◽  
I. D. Dada ◽  
A. A. Orimogunje ◽  
...  

This study evaluated the effect of phytogenic feed additives (Lemon peel, LP and Orange peel, OP) added singly or in combination to basal diets on the performance, gut morphology, haematology, serum biochemistry and lipid profile of broiler chickens. Four nutritionally adequate Corn-Soybean based diets were generated by the addition of LPand/or OPto basal diet. A total of 320, 1-d-old Marshall strain broiler chicks were given the basal diet supplemented with no additive (T1), 500g/tonne of LP (T2), OP (T3), or LP+OP (T4) in a study which lasted for 42 days. Growth performance was assessed weekly while gut and blood samples were collected at d 42 for examination. Data generated were analyzed using ANOVA with a P<0.05 level of significance. Results showed that chickens fed diets supplemented with LP and OP had similar (P>0.05) growth performance compared with the control group. Higher (P<0.05) duodenal and jejunal villus height were observed in chickens fed LP and OP, respectively. Phytogenics inclusion caused reduced (P<0.05) laminal propria depth at the ileum segment. Higher (P<0.05) PCV (30.00%), total protein (6.89 g/L) and albumin (3.67 g/L) were observed in chickens on LP+OP. Haemoglobin concentration (Hb) and RBC were not affected by the treatments imposed. Serum creatinine, uric acid and liver enzymes (AST and ALT) activity were reduced (P<0.05) with inclusion of LP and OP in broiler diets. Increase (P<0.05) HDL and decrease total cholesterol, LDL and triglycerides were observed in broilers fed LP and OP. Dietary inclusion of LP and OP had positive influence on gut morphology, RBC production, plasma protein reserves and lipid profile of broiler chickens.


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