scholarly journals Harvest Season Significantly Influences the Fatty Acid Composition of Bee Pollen

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 495
Author(s):  
Saad N. Al-Kahtani ◽  
El-Kazafy A. Taha ◽  
Soha A. Farag ◽  
Reda A. Taha ◽  
Ekram A. Abdou ◽  
...  

Seasonal variations in the fatty acid (FA) compositions of pollen loads collected from the Al-Ahsa Oasis in eastern Saudi Arabia throughout one year were determined to identify the optimal season for harvesting bee pollen rich in essential fatty acids (EFAs) and unsaturated fatty acids (UFAs). The highest values (%) of lipids, linolenic acid (C18:3), stearic acid (C18:0), linoleic acid (C18:2), arachidic acid (C20:0), the sum of the C18:0, C18:1, C18:2, and C18:3 concentrations, and EFAs were obtained from bee pollen harvested during autumn. The maximum values (%) of oleic acid (C18:1), palmitic acid (C16:0), UFAs, and the UFA/saturated fatty acid (SFA) ratio were found in bee pollen harvested during summer. The highest concentrations (%) of behenic acid (C22:0), lignoceric acid (C24:0), and SFAs were found in bee pollen harvested during winter. Bee pollen harvested during spring ranked second in its oleic, palmitic, linolenic, stearic, arachidic, behenic, and lignoceric acid concentrations and for EFAs, UFAs, and the UFA/SFA ratio. The lowest SFA concentration was found in bee pollen harvested during summer. Oleic, palmitic, and linolenic acids were the most predominant FAs found in bee pollen. It was concluded that the FA composition of bee pollen varied among the harvest seasons due to the influence of the dominant botanical origins. We recommend harvesting pollen loads during spring and summer to feed honeybee colonies during periods of scarcity and for use as a healthy, nutritious food for humans.

2012 ◽  
Vol 66 (2) ◽  
pp. 207-209 ◽  
Author(s):  
Boris Pejin ◽  
Ljubodrag Vujisic ◽  
Marko Sabovljevic ◽  
Vele Tesevic ◽  
Vlatka Vajs

The fatty acid composition of the moss species Atrichum undulatum (Hedw.) P. Beauv. (Polytrichaceae) and Hypnum andoi A.J.E. Sm. (Hypnaceae) collected in winter time were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) as a contribution to their chemistry. Eight fatty acids were identified in the chloroform/methanol extract 1:1 of A. undulatum (linoleic acid 26.80%, palmitic acid 22.17%, ?-linolenic acid 20.50%, oleic acid 18.49%, arachidonic acid 6.21%, stearic acid 3.34%, cis-5,8,11,14,17-eicosapentaenoic acid 1.52% and behenic acid 1.01%), while six fatty acids were found in the same type of extract of H. andoi (palmitic acid 63.48%, erucic acid 12.38%, stearic acid 8.08%, behenic acid 6.26%, lignoceric acid 5.16% and arachidic acid 4.64%). According to this study, the moss A. undulatum can be considered as a good source of both essential fatty acids for humans (linoleic acid and ?-linolenic acid) during the winter.


2001 ◽  
Vol 47 (5) ◽  
pp. 382-391 ◽  
Author(s):  
Hongjun He ◽  
Roger Gordon ◽  
John A Gow

In the first part of this study, generation times relative to temperature, together with cardinal and conceptual temperatures, were determined for four strains of Xenorhabdus bacteria that represented three geographically distinct species. The data showed that the NF strain of Xenorhabdus bovienii, like the Umeå strain of the same species, is psychrotrophic, while Xenorhabdus sp. TX strain resembles Xenorhabdus nematophila All strain in being mesophilic. In the second part, the capacity of these bacteria to adapt to changes in temperature, shown by changes in fatty acid composition, was investigated. As temperature declined, the proportions of the two major unsaturated fatty acids, palmitoleic (16:1ω7) acid and oleic (18:1ω9) acid, increased significantly in all of the strains. The proportion of the prevalent saturated fatty acid, which was palmitic acid (16:0), decreased. In the All, NF, and Umeå strains, myristic acid (14:0), margaric acid (17:0), cyclopropane (17:0c), and arachidic acid (20:0) decreased with decreasing temperature. In the third part of the study, the synthesis of isozymes in response to changing temperature was investigated. For the seven enzymes studied, the numbers for which isozyme synthesis was temperature related were as follows: five for Umeå, four for All, three for NF, and two for TX. Where the study dealt with fatty acid composition and isozyme synthesis, the results show a broad capacity for physiological temperature adaptation among strains of different climatic origin.Key words: Xenorhabdus, temperature, psychrotroph, mesophile, fatty acid, isozyme.


2018 ◽  
Vol 21 (3) ◽  
pp. 549 ◽  
Author(s):  
Lorde Sembiring ◽  
Mirna Ilza ◽  
Andarini Diharmi

Catfish (Pangasius hypophthalmus) processing industry produces by products of abdoment fat containing unsaturated fatty acids, polyunsaturated fatty acids (PUFAs) which are the essential fatty acids needs to maintain health. Catfish belly fat can be processed into coarse fish oil through a purification process, with the addition of the adsorbent used, one of which is bentonite. This study was aimed to determine the<br />effect of bentonite application on the characteristics of refined catfish oil. Fish oil was extracted from belly fat then purified by adding bentonite adsorbent at a concentration of 1; 4 and 7%. The design used in this<br />study was a complete factorial of one factor. The parameters of analysis were the number of free fatty acids, peroxide value, anisidine, and totox. The results showed that bentonite had significant effect on free fatty<br />acid, peroxide, anisidine and totox values. The use of 7% bentonite decreased content of free fatty acid, peroxide value, anisidine, peroxide and totox from 1.72 to 0.85%, 5.18 to 0 meq/kg, 27.51 to 2.28 meq/kg,<br />and 37.88 to 2.28 meq/kg respectively.


Author(s):  
Muhammad Farhan Jahangir Chughtai ◽  
Imran Pasha ◽  
Faqir Muhammad Anjum ◽  
Muhammad Adnan Nasir

Sorghum and millet are important food staples in semi-arid tropics of Asia and Africa. Sorghum and millet are cereal grains that have prospective to be used as substitute to wheat flour for celiac patients. These are considered as the good source of many important and essential fatty acids. The volatile profiling of these two important crops is comparable to other cereals as well. The present study was an effort to explore biochemical composition of commercially available sorghum and millet varieties with special reference to their fatty acid and volatile profiling. Chemical composition of sorghum and millet was determined according to respective methods. Fatty acid methyl esters were prepared and then subjected to GC-FID for fatty acids analysis. The results indicated that both sorghum and millet oils are rich in essential fatty acids comprising mono and polyunsaturated fatty acids. Main fatty acids that are identified in current study includes palmitic acid, oleic acid, palmitoleic acid, behenic acid, linoleic acid, linoleic acid, stearic acid, myristic acid, etc. On the other hand volatile compounds from sorghum and millet were determined by preparing their respective volatile samples by using calvenger apparatus with suitable volatile extracting solvent. Volatile samples were then subjected to GC-MS analysis and respected results were compared with NIST library. About 30 different volatiles were identified in millet varieties while 35 different compounds were discovered in sorghum varieties belonging to aldehydes, ketones, benzene derivatives, esters, alcohols, sulphur compounds.


2019 ◽  
Vol 150 (2) ◽  
pp. 340-349 ◽  
Author(s):  
Andres V Ardisson Korat ◽  
Vasanti S Malik ◽  
Jeremy D Furtado ◽  
Frank Sacks ◽  
Bernard Rosner ◽  
...  

ABSTRACT Background Very-long-chain SFAs (VLCSFAs), such as arachidic acid (20:0), behenic acid (22:0), and lignoceric acid (24:0), have demonstrated inverse associations with cardiometabolic conditions, although more evidence is needed to characterize their relation with risk of type 2 diabetes (T2D). In addition, little is known regarding their potential dietary and lifestyle predictors. Objective We aimed to examine the association of plasma and erythrocyte concentrations of VLCSFAs with incident T2D risk. Methods We used existing measurements of fatty acid concentrations in plasma and erythrocytes among 2854 and 2831 participants in the Nurses’ Health Study (NHS) and Health Professionals Follow-Up Study (HPFS), respectively. VLCSFAs were measured using GLC, and individual fatty acid concentrations were expressed as a percentage of total fatty acids. Incident T2D cases were identified by self-reports and confirmed by a validated supplementary questionnaire. Cox proportional hazards regression was used to evaluate the association between VLCSFAs and T2D, adjusting for demographic, lifestyle, and dietary variables. Results During 39,941 person-years of follow-up, we documented 243 cases of T2D. Intakes of peanuts, peanut butter, vegetable fat, dairy fat, and palmitic/stearic (16:0–18:0) fatty acids were significantly, albeit weakly, correlated with plasma and erythrocyte VLCSFA concentrations (|rs| ≤ 0.19). Comparing the highest with the lowest quartiles of plasma concentrations, pooled HRs (95% CIs) were 0.51 (0.35, 0.75) for arachidic acid, 0.43 (0.28, 0.64) for behenic acid, 0.40 (0.27, 0.61) for lignoceric acid, and 0.41 (0.27, 0.61) for the sum of VLCSFAs, after multivariate adjustments for demographic, lifestyle, and dietary factors. For erythrocyte VLCSFAs, only arachidic acid and behenic acid concentrations were inversely associated with T2D risk. Conclusions Our findings suggest that, in US men and women, higher plasma concentrations of VLCSFAs are associated with lower risk of T2D. More research is needed to understand the mechanistic pathways underlying these associations.


1987 ◽  
Vol 33 (10) ◽  
pp. 1869-1873 ◽  
Author(s):  
E N Siguel ◽  
K M Chee ◽  
J X Gong ◽  
E J Schaefer

Abstract To develop criteria for deficiency of essential fatty acids (EFA), we used capillary-column gas-liquid chromatography to determine fatty acids (percentage of total fatty acids) in plasma obtained in the fasting state from 56 reference subjects and from 10 patients with intestinal fat malabsorption and suspected EFA deficiency. Fatty acid evaluations (percentage of total fatty acids) that allowed for a clear distinction (P less than 0.01) between reference subjects and patients, based on values two standard deviations below or above the reference mean, included values for linoleic acid (18:2w6) below 27%, and values for palmitic acid (16:0), palmitoleic acid (16:1w7), oleic acid (18:1w9), vaccenic acid (18:1w7), and Mead acid (20:3w9) exceeding 21%, 2.6%, 23.3%, 2.1%, and 0.21%, respectively. Ratios of total EFA to total non-EFA of less than 0.60 and of Mead acid to arachidonic acid of greater than 0.025 also served to identify patients, and were not found in reference subjects. Significant inverse correlations between percentages of plasma EFA and plasma mono-unsaturated fatty acids were noted. Our reference-interval data can be used to assess normality of plasma EFA status.


2020 ◽  
Author(s):  
Wenjiao Shi ◽  
Yuanzhi Peng ◽  
Jianer Du ◽  
Xiaojian Weng

Abstract Visceral adipose tissue (VAT) is the main source of circulating fatty acids (FAs) that provides the energy substrate for the heart. Till now, studies have not shown a clear association between individual circulating FAs and heart failure (HF). In this study, we aimed to investigate the changes in circulating FAs in HF mice and their association with VAT by removing epididymal white adipose tissue (eWAT). Here, we found that the serum levels of four fatty acids, namely arachidic acid, behenic acid, lignoceric acid, and docosapentaenoic acid, were significantly decreased in pressure-induced HF mice via transverse aortic constriction (TAC) surgery accompanied with cardiac enlargement and fibrosis. Most importantly, removal of eWAT in mice led to a significant decrease in the levels of the above-mentioned fatty acids without any significant difference between the HF and sham groups. Accordingly, cardiac enlargement and fibrosis were significantly alleviated. We concluded that VAT excision alleviated TAC-induced cardiac failure by decreasing serum arachidic acid, behenic acid, lignoceric acid, and docosapentaenoic acid levels.


2021 ◽  
Vol 69 (1) ◽  
pp. 59-62
Author(s):  
Md Rubel Al Mamun ◽  
Tasnim Ahmed ◽  
Md Selim Aktar Reza ◽  
Md Hasanur Rahman

Ethylacetate extract of the roots of Amaranthus spinosus L. was subjected to phytochemical investigation and three compounds stigmasterol, 1-Eicosanol and oleic acid were isolated in pure state. The n-hexane extract was analysed for fatty acid with GC-FID and four saturated fatty acids; caprylic acid, stearic acid, arachidic acid and behenic acid, and four unsaturated fatty acids; palmitoleic acid, oleic acid, linoleic acid and erucic acid were identified and quantified. Different extracts were assessed to explore their in vitro membrane stabilizing activity using standard protocol. Methanol extract of A. spinosus showed 68.13% inhibition in hypotonic solution-induced hemolysis and 74.53% inhibition in heat induced hemolysis which was the highest than its other Kupchan fractions. Acetyl salicylic acid was used as standard that showed 42.00% inhibition of hemolysis at normal condition. Dhaka Univ. J. Sci. 69(1): 59-62, 2021 (January)


2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Zaha A. Elagbar ◽  
Rajashri R. Naik ◽  
Ashok K. Shakya ◽  
Sanaa K. Bardaweel

The total oil yield and the fatty acid composition were determined in theAnnona muricataL. fixed oil using organic solvent extraction and GC-FID. The seeds were found to contain about ~21.5% of crude fixed oil on a dry weight basis. The crude oil containing fatty acid was converted into methyl esters and analysed by GC-FID. Fourteen fatty acids were identified using GC-FID. The major monounsaturated and saturated fatty acids were oleic acid (39.2%) and palmitic acid (19.1–19.2%), respectively, whereas theα-linolenic acid (1.2%) and linoleic acid (34.9%) were polyunsaturated fatty acid. The other saturated acids were stearic acid (3.3%), arachidic acid (0.4%), myristic acid (0.1%), heptadecanoic acid (0.1%), behenic acid (0.1%), and lignoceric acid (0.1%). Some of the fatty acids have not been reported earlier from the oil ofAnnona muricataL. Fixed oil exhibited significant free radical scavenging activity which was measured using DPPH and is also known to inhibit the gastrointestinal motility significantly.


Author(s):  
Vladimir Viktorovich Mungin ◽  
Ludmila Nikolaevna Loginova ◽  
Ekaterina Aleksandrovna Aryukova ◽  
Bibigul Mahabbatovna Kurkembaeva ◽  
Anna Aleksandrovna Bakhareva

Fats play a critical role in energy metabolism of fish. The bulk of the fatty acids of fish lipids are saturated with fatty acids and highly unsaturated acids with a predominance of 18 carbon atoms of mostly oleic acid, linoleic acid and their isomers. During oxidation they liberate two times more energy and, being a source of essential fatty acids, account for the complex basis of cell membranes. Efficiency of tissue permeability and its adaptation to different temperatures depend on lipids. The composition and ratio of fatty acids depend on a number of factors, including biological characteristics of the organism (age, species) and external environment influence (time of the year, temperature, water salinity). Fluctuations of fat content in one and the same individual during the year can be considerable and these fluctuations are repeated regularly. In addition, fats are related to the fish intake of fat-soluble physiologically active substances. The article presents the results of fatty acid composition of fish blood, depending on body mass and seasonal changes. Change levels of saturated and unsaturated fatty acids are shown in the seasonal aspect. Blood fatty-acid composition of carps in the lakes of the Republic of Mordovia is represented mostly by omega-3, -6, -9 fatty acids. It has been stated that if the number of fatty acid radicals increase or decrease, an organism adopts to the temperature changes, which helps to survive within the areal.


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