scholarly journals Functional Microbiota for Polypeptide Degradation during Hypertonic Moromi-Fermentation of Pixian Broad Bean Paste

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 930
Author(s):  
Lijie Zhang ◽  
Yida Bao ◽  
Haifeng Chen ◽  
Jiaquan Huang ◽  
Yan Xu
Keyword(s):  
Amino Acid ◽  
High Throughput Sequencing ◽  
Raw Material ◽  
Asian Countries ◽  
Spontaneous Fermentation ◽  
Second Stage ◽  
Extracellular Proteome ◽  
Acid Accumulation ◽  
Two Stages ◽  
Candida Zeylanoides

Traditional fermented bean pastes are indispensable seasonings in many East Asian countries. They are produced via hypertonic solutions by spontaneous fermentation. Functional, unknown microbiota carry great risks for food safety and stable quality. Thus, analysis and subsequent utilization of functional microbiota will be a good strategy to resolve these problems. During bean fermentation, the microbial functions were divided into two stages, including first stage-raw material (polypeptide) degradation and second stage-amino acid catabolism. In this study, we aimed to analyze the functional microbiota of first stage. Omics-studies, including high-throughput sequencing, correlation analysis and extracellular proteome, were used to generate candidate functional microbes for polypeptide degradation in this study. Then, we cultured the candidate functional microbes. After the batch fermentation and enzymatic analysis, we found three strains secreted peptidase and resulted amino acid accumulation, involving Aspergillus niger, Candida zeylanoides and Bacillus licheniformis. Thus, A. niger, C. zeylanoides and B. licheniformis conducted the functional microbiota for polypeptide degrading during hypertonic moromi fermentation. This study supplies a strategy for functional microbiota analysis. In addition, this is the first report that C. zeylanoides can secrete proteome and produce amino acids from polypeptide.

HEMATOPOETIC STEM CELL RESPONSE TO STIMULATION OF EXTRACTS FROM ANIMAL MATERIALS

2016 ◽  
Vol 78 (5-6) ◽  
Author(s):  
Ivan Smirnov ◽  
Victor Keino ◽  
Ksenia Goryacheva ◽  
Alexander Shunk ◽  
Alexander Bondarev ◽  
...  
Keyword(s):  
Raw Materials ◽  
Raw Material ◽  
Animal Origin ◽  
Aqueous Extracts ◽  
Second Stage ◽  
Hematopoetic Stem Cell ◽  
Two Stages ◽  
Stimulation Of

The article presents the results of the research hemostimulating activity of aqueous extracts of antler young Siberean stag and drone larvae homogenate. These substrates were obtained from raw materials of animal origin. Altai Krai andAltaiRepublicare subjects of theRussian Federationwhich is the place of production of the raw material. Experiments were conducted in two stages. The first stage - in vitro, which included a research of experimental substrates on the culture of mouse marrow cells. During the experiments were obtained different results. We counted the number of colonies grown in cell culture for this. The second stage of experimenters - in vivo. It included an assessment of the myeloprotector on model of cytostatic myelosuppression of mice and analysis of bone marrow and peripheral blood.

scholarly journals Penjaminan Keberlanjutan Pembangunan Kapal Kayu Melalui Pelatihan Pembuatan Pola Gading untuk Penerapan Inovasi Gading Baja Sebagai Pengganti Gading Kayu bagi Pengrajin Kapal Kayu di Kabupaten Takalar

2019 ◽  
Vol 2 (1) ◽  
pp. 70-77
Author(s):  
Mohammad Rizal Firmansyah ◽  
Lukman Bochary ◽  
Syamsul Asri ◽  
Muhammad Rusydi Alwi ◽  
Rosmani Rosmani ◽  
...  
Keyword(s):  
Alternative Solution ◽  
Raw Material ◽  
Curved Beams ◽  
Special Shape ◽  
Second Stage ◽  
South Sulawesi ◽  
Fishing Vessels ◽  
Service Activity ◽  
Two Stages

Currently wood raw material used for ivory or wooden ship frames is increasingly difficult to obtain because it has a special shape, where the wood for the ship's ivory must be taken from the tree in the form of curved beams, so that it is easily shaped according to the shape of the wooden ship that will be built. Some wooden ship craftsmen in South Sulawesi have faced this difficulty. One of them is a wooden ship craftsman who is part of a group of wooden ship craftsmen "TORANI" in Galesong Kota Village, Galesong District, Takalar. This group of craftsmen specifically produces fishing vessels with sizes of 10 GT to 30 GT. To overcome this problem, an alternative solution for wood ivory is needed. A study has found an alternative substitute for wood ivory, namely steel, with dimensions suitable for use on fishing vessels of 10 GT, 20 GT, and 30 GT. The dimensions of the steel ivory obtained are close to the wood ivory but with greater strength. To be able to apply the steel ivory to the wooden ship, the partner craftsmen need to be trained in its use, starting from pattern taking, making and installing steel ivory. The target of partner activities in this service activity is in the first stage of the process of applying steel ivory to wooden ships, which is capable of making steel ivory patterns. This pattern will later be used as the basis for making steel ivory on the wooden ships they built. The method of the activity carried out was training and mentoring for partner members to make steel ivory patterns in two stages as follows: The first stage was counseling and introduction to partners regarding steel ivory as a substitute for wood ivory; and the second stage is the practice and accompaniment of technology for making steel ivory patterns. The second stage includes guiding participants using equipment and equipment for making ivory patterns and guiding the practice of making steel ivory patterns taken from the shape of wooden ships by following the curves of the wooden ship's skin.

scholarly journals PENURUNAN JUMLAH CACAT DAN BIAYA BAHAN BAKU DENGAN METODE ResponsESPONSSE SURFACE

2014 ◽  
Vol 14 (2) ◽  
pp. 139
Author(s):  
Muhammad Lukman ◽  
Ahmad Zubaid Firdaus ◽  
Rahadian Galih
Keyword(s):  
Raw Materials ◽  
Poor Quality ◽  
Raw Material ◽  
Optimal Amount ◽  
Second Stage ◽  
Control Factors ◽  
Surface Method ◽  
Optimal Setting ◽  
Two Stages ◽  
A Company

Muhammad Lukman, Ahmad Zubaid Firdaus, Rahadian GalihJurusan Teknik Industri, Fakultas Teknik, Universitas Muhammadiyah MalangJl. Raya Tlogomas No. 246 MalangLaman : [email protected] Indah Cemerlang adalah salah satu perusahaan yang bergerak dalam bidang industri bahan bangunan yaitu produk paving. Dalam proses produksi perusahaan sering melakukan perubahan komposisi bahan baku yang dipengaruhi oleh biaya bahan baku. Faktor perubahan komposisi bahan baku ini membuat kualitas mutu produk mengalami penurunan. Oleh karena itu perlu diadakan suatu penelitian untuk mengidentifikasi faktor-faktor kontrol yang berpengaruh terhadap respons cacat dengan metode Responsse Surface. Penelitian dengan metode Responsse Surface ini mempunyai dua tahap. Tahap pertama yaitu orde 1 (screening) dan tahap kedua yaitu orde 2 (optimization). Hasil orde 1 diketahui komposisi bahan baku yang memengaruhi respons cacat yaitu volume semen dan abu batu. Berdasarkan hasil tersebut maka pada tahap kedua yaitu orde 2 dilakukan percobaan untuk menentukan komposisi bahan baku yang optimal. Setting komposisi bahan baku terbaik yaitu air 20 liter, semen 47 kg, pasir 120 kg, abu batu 10,45 takaran, dengan nilai respons kecacatan sebesar 5,08 persen. Penurunan biaya bahan baku dengan menggunakan komposisi tersebut sebesar Rp49,33 per palet dan biaya kerugian kualitas mengalami penurunan sebesar Rp47.640 per minggu.Kata kunci : metode responsse surface, cacat, optimasiABSTRACTCV.   Indah Cemerlang  is a  company  for building material which  produces paving  stone. The company  often  changes  the raw materials composition which is affected  by  cost  of raw materials. Change of  the raw  materials composition  makes  product quality  lower. Therefore, a study should be conducted in order to identify  control factors  influencing on defect responsse by using Responsse  Surface  method. Responsse surface method used in this research consists of two stages. First stage, called orde 1, is dealing with screening and second stage, called orde 2, is dealing with optimization. The result of orde 1 shows that the raw materials composition influencing on defect responsse are cement volume and gray stone. According to the result of second stage, the experiment is conducted to determine optimal amount of raw materials composition. Optimal amount of the raw materials composition are 20 liters of water, 47 kilograms of cement, 120 kilograms of sand, and 10.45 doses of gray stone, with value of defect responsse 5.08%. Applying proposed composition can decrease cost of raw material by Rp 49,33 per pallet and cost of poor quality by 47.60 per week.Key words: responsse surface method, defect, optimization

Altered Fine Structure of B Lymphocytes Correlated with Defective Terminal Differentiation

1973 ◽  
Vol 31 ◽  
pp. 386-387
Author(s):  
Dale E. Bockman ◽  
L. Y. Frank Wu ◽  
Alexander R. Lawton ◽  
Max D. Cooper
Keyword(s):  
Immune Deficiency ◽  
B Lymphocytes ◽  
Plasma Cells ◽  
Present Report ◽  
Specific Antigen ◽  
Terminal Differentiation ◽  
Second Stage ◽  
Two Stages ◽  
Deficiency Diseases ◽  
Cell Line Generation

B-lymphocytes normally synthesize small amounts of immunoglobulin, some of which is incorporated into the cell membrane where it serves as receptor of antigen. These cells, on contact with specific antigen, proliferate and differentiate to plasma cells which synthesize and secrete large quantities of immunoglobulin. The two stages of differentiation of this cell line (generation of B-lymphocytes and antigen-driven maturation to plasma cells) are clearly separable during ontogeny and in some immune deficiency diseases. The present report describes morphologic aberrations of B-lymphocytes in two diseases in which second stage differentiation is defective.

Distributed Entropy Energy-Efficient Clustering algorithm for cluster head selection (DEEEC)

2020 ◽  
Vol 39 (6) ◽  
pp. 8139-8147
Author(s):  
Ranganathan Arun ◽  
Rangaswamy Balamurugan
Keyword(s):  
Energy Efficient ◽  
Clustering Algorithm ◽  
Cluster Head ◽  
Residual Energy ◽  
Energy Utilization ◽  
Sensor Nodes ◽  
Second Stage ◽  
Energy Efficient Clustering ◽  
Two Stages ◽  
Ch Selection

In Wireless Sensor Networks (WSN) the energy of Sensor nodes is not certainly sufficient. In order to optimize the endurance of WSN, it is essential to minimize the utilization of energy. Head of group or Cluster Head (CH) is an eminent method to develop the endurance of WSN that aggregates the WSN with higher energy. CH for intra-cluster and inter-cluster communication becomes dependent. For complete, in WSN, the Energy level of CH extends its life of cluster. While evolving cluster algorithms, the complicated job is to identify the energy utilization amount of heterogeneous WSNs. Based on Chaotic Firefly Algorithm CH (CFACH) selection, the formulated work is named “Novel Distributed Entropy Energy-Efficient Clustering Algorithm”, in short, DEEEC for HWSNs. The formulated DEEEC Algorithm, which is a CH, has two main stages. In the first stage, the identification of temporary CHs along with its entropy value is found using the correlative measure of residual and original energy. Along with this, in the clustering algorithm, the rotating epoch and its entropy value must be predicted automatically by its sensor nodes. In the second stage, if any member in the cluster having larger residual energy, shall modify the temporary CHs in the direction of the deciding set. The target of the nodes with large energy has the probability to be CHs which is determined by the above two stages meant for CH selection. The MATLAB is required to simulate the DEEEC Algorithm. The simulated results of the formulated DEEEC Algorithm produce good results with respect to the energy and increased lifetime when it is correlated with the current traditional clustering protocols being used in the Heterogeneous WSNs.

Analisis Kebutuhan Petunjuk Praktikum Berbasis Keterampilan Proses Sains untuk Mencapai Kemampuan Merancang Eksperimen

2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Fitriah Khoirunnisa ◽  
Friska Septiani Silitonga ◽  
Veri Firmansyah
Keyword(s):  
Core Competence ◽  
Process Skills ◽  
Learning Materials ◽  
Science Process Skills ◽  
Second Stage ◽  
The Core ◽  
The Core Competence ◽  
Two Stages ◽  
Practical Instruction ◽  
Design Experiments

Penelitian ini bertujuan menganalisis kebutuhan petunjuk praktikum berbasis Keterampilan Proses Sains (KPS) untuk mencapai kemampuan merancang eksperimen pada materi kalor reaksi kalorimetri. Penelitian dilakukan terhadap peserta didik kelas XI SMA Negeri 2 Kota Tanjungpinang. Variabel penelitian mencakup analisis kebutuhan bahan ajar dan analisis kesesuaian Kompetensi Inti (KI) dan Kompetensi Dasar (KD). Jenis penelitian yang dilakukan adalah penelitian deskriptif kualitatif. Tahapan pertama dalam penelitian ini adalah menganalisis kebutuhan bahan ajar dengan cara membandingkan dua petunjuk praktikum yang selama ini telah digunakan di sekolah tersebut, ditinjau dari aspek struktur format penulisan, aspek kreativitas, dan aspek keterampilan proses sains yang terdapat dalam petunjuk praktikum. Sehingga didapatkan kesimpulan bahwa petunjuk praktikum yang selama ini digunakan tidak memberikan kesempatan kepada peserta didiknya untuk merancang eksperimen yang telah ditentukan. Tahapan kedua yaitu menganalisis kesesuaian kompetensi inti dan kompetensi dasar, yang bertujuan untuk menentukan indikator pencapaian kompetensi (IPK) yang akan menjadi acuan dalam mengembangkan petunjuk praktikum berbasis keterampilan proses sains. Dari kedua tahapan yang telah dilakukan maka dapat disimpulkan bahwa peserta didik memerlukan petunjuk praktikum yang mampu mengonstruksi pikiran dan mengaktifkan kinerja mereka, sehingga pendekatan Keterampilan Proses Sains menjadi pilihan dalam mengembangkan petunjuk praktikum yang sesuai dengan karakteristik kurikulum 2013.   This research aims to analyze the needs of Science Process Skills based Practical Instruction to achieve the ability to design experiments on the calor of reaction. This research was done to the students of class XI SMA Negeri 2 Tanjungpinang City. Research Variable includes the analysis of the needs of the learning materials and analysis of the suitability of the Core Competence (KI) and Basic Competence (KD). The type of research conducted is descriptive qualitative research. The first stages in this research is to analyze the needs of learning materials by comparing two practical instructions that had been implementing in the school, from the aspects of the structure of writing format, creativity, and science process skills embedded in practical instructions. The conclusion of this research that current practical instructions does not give an opportunity to the participants to design determined experiments. The second stage, namely analyzing the suitability of core competence and basic competence, which aims to determine the indicators of achievement of the competencies (GPA) which will be a reference in developing process skills-based teaching instructions science. Of the two stages that has been done then it can be concluded that learners need practical instructions to construct  thinking and and their performance, so the Science Process Skills approach is an option in developing practical instruction suitable for the characteristics of the curriculum of 2013.

ESTIMATING GAS AND ROCK OUTBURST RISK ON THE BASIS OF KNOWLEDGE AND EXPERIENCE – THE EXPERT SYSTEM BASED ON FUZZY LOGIC

2014 ◽  
Vol 59 (1) ◽  
pp. 41-52 ◽  
Author(s):  
Norbert Skoczylas
Keyword(s):  
Fuzzy Logic ◽  
Expert System ◽  
Mining Industry ◽  
Automatic Assessment ◽  
Science Field ◽  
Second Stage ◽  
Two Stages ◽  
Outburst Hazard

Abstract The Author endeavored to consult some of the Polish experts who deal with assessing and preventing outburst hazards as to their knowledge and experience. On the basis of this knowledge, an expert system, based on fuzzy logic, was created. The system allows automatic assessment of outburst hazard. The work was completed in two stages. The first stage involved researching relevant sources and rules concerning outburst hazard, and, subsequently, determining a number of parameters measured or observed in the mining industry that are potentially connected with the outburst phenomenon and can be useful when estimating outburst hazard. Then, the Author contacted selected experts who are actively involved in preventing outburst hazard, both in the industry and science field. The experts were anonymously surveyed, which made it possible to select the parameters which are the most essential in assessing outburst hazard. The second stage involved gaining knowledge from the experts by means of a questionnaire-interview. Subjective opinions on estimating outburst hazard on the basis of the parameters selected during the first stage were then systematized using the structures typical of the expert system based on fuzzy logic.

Integrated assessment and mapping of the environmental situation in the Russian Federation regions

2017 ◽  
Vol 924 (6) ◽  
pp. 6-16
Author(s):  
V.S. Tikunov ◽  
O.Yu. Chereshnia
Keyword(s):  
Russian Federation ◽  
Pollution Index ◽  
Pollution Level ◽  
Anthropogenic Pressure ◽  
Environmental Capacity ◽  
Environmental Friendliness ◽  
Second Stage ◽  
Environmental Situation ◽  
The Russian Federation ◽  
Two Stages

The article presents a methodology for a comprehensive assessment of the environmental situation in Russian Federation regions based on the pollution index and the index of the ecological tension. The evaluation was carried out in two stages. At the first stage, the degree of pollution of the atmosphere, hydrosphere and lithosphere of the regions was estimated on the basis of the emission of pollutants into the atmosphere, departing from stationary sources, the formation of solid domestic wastes (SDW) and the discharge of contaminated wastewater. Based on these three indicators, a pollution index was constructed that estimates aggregate pollution level. In the second stage, the authors made the estimation of loads generated by atmospheric emissions, solid waste and waste water discharged into the territory of each region, per capita and in relation to the environmental capacity of the economy. This allows us to take into account the area of pollution, anthropogenic pressure and environmental responsibility of the population, as well as the environmental friendliness of production. On the basis of relative indicators, the index of ecological tension was created.

scholarly journals The Psychology behind Knowledge Hiding in an Organization

2021 ◽  
Vol 11 (2) ◽  
pp. 57
Author(s):  
Roksana Binte Rezwan ◽  
Yoshi Takahashi
Keyword(s):  
Knowledge Management ◽  
Literature Review ◽  
Systematic Literature Review ◽  
Personal Goals ◽  
Psychological Process ◽  
Personal Goal ◽  
Second Stage ◽  
Management Literature ◽  
Two Stages ◽  
Knowledge Hiding

This study aimed to understand the psychological process behind employees’ knowledge hiding (KH) behaviors in organizations. KH is an intentional act of concealing knowledge when it is requested by a colleague and can lead to counterproductive consequences for the organization. Therefore, this study synthesized previous studies (n = 88) on KH through a systematic literature review. We used the cognitive–motivational–relational (CMR) theory of emotion to create a framework for the studies’ findings. Based on the framework, the psychological process behind KH has two stages—personal goal generation and the knowledge-request event appraisal process, each of which contains its own CMR process. In the first stage, an individual’s internal and external attributes related to the organization shape their personal goals. In the second stage, an individual appraises the features of a knowledge-request event in terms of both their personal goal and the internal and external attributes that created the goal. If the knowledge request is appraised as harmful for the personal goal, emotion arises and leads to the manifestation of KH. This study contributes to the knowledge management literature as, to our knowledge, it is the first to propose a CMR theory-based framework to understand the overall psychological process behind KH.

scholarly journals High-Throughput Sequencing of Phage Display Libraries Reveals Parasitic Enrichment of Indel Mutants Caused by Amplification Bias

2021 ◽  
Vol 22 (11) ◽  
pp. 5513
Author(s):  
Sander Plessers ◽  
Vincent Van Deuren ◽  
Rob Lavigne ◽  
Johan Robben
Keyword(s):  
Amino Acid ◽  
Phage Display ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Target Selection ◽  
Acid Modification ◽  
Phage Display Technology ◽  
Amplification Bias ◽  
Large Deletions ◽  
Depth Analysis

The combination of phage display technology with high-throughput sequencing enables in-depth analysis of library diversity and selection-driven dynamics. We applied short-read sequencing of the mutagenized region on focused display libraries of two homologous nucleic acid modification eraser proteins—AlkB and FTO—biopanned against methylated DNA. This revealed enriched genotypes with small indels and concomitant doubtful amino acid motifs within the FTO library. Nanopore sequencing of the entire display vector showed additional enrichment of large deletions overlooked by region-specific sequencing, and further impacted the interpretation of the obtained amino acid motifs. We could attribute enrichment of these corrupted clones to amplification bias due to arduous FTO display slowing down host cell growth as well as phage production. This amplification bias appeared to be stronger than affinity-based target selection. Recommendations are provided for proper sequence analysis of phage display data, which can improve motive discovery in libraries of proteins that are difficult to display.

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