scholarly journals Optimization of Ultrasonic Extraction to Obtain Erinacine A and Polyphenols with Antioxidant Activity from the Fungal Biomass of Hericium erinaceus

Foods ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1889
Author(s):  
Mihai-Vlad Valu ◽  
Liliana Cristina Soare ◽  
Nicoleta Anca Sutan ◽  
Catalin Ducu ◽  
Sorin Moga ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Extraction Procedure ◽  
Natural Antioxidants ◽  
Extraction Process ◽  
Fungal Species ◽  
Extraction Yield ◽  
Ultrasonic Extraction ◽  
Hericium Erinaceus ◽  
High Antioxidant Activity ◽  
Erinacine A

Hericium erinaceus is a medicinal fungal species that produces the active biological metabolite erinacine A with strong antioxidant activity. The classical extraction techniques used to date to obtain metabolites from this fungal species require high consumption of resources and energy and, in the end, prove to be expensive and inefficient, especially on a biomedical scale. The aim of this research is based on the development of an ultrasonic extraction (UE) method for the identification and extraction of biological compounds with high antioxidant activity from the mycelia of H. erinaceus biomass developed through a solid cultivation process. The extraction process was optimized by varying parameters to determine the best extraction yield of metabolites involved in such antioxidant activity, using the response surface methodology (RSM). The physicochemical analyses were oriented towards the investigation of polyphenols, flavonoids, and the diterpenoid erinacine A. It is highlighted that there is a very good mutual connection between the concentration of polyphenols and flavonoids in the extracts studied and the diterpenoid erinacine A. Also, this study describes an efficient and qualitative extraction method for extracting natural antioxidants from the H. erinaceus mushroom, since toxic solvents were not used in the developed extraction procedure. This biomass can be used both as a food source and as a possible phytotherapeutic tool in the prevention or treatment of various neurodegenerative disorders that require drugs with strong antioxidant activity.

scholarly journals Water–Organic Solvent Extraction of Phenolic Antioxidants from Brewers’ Spent Grain

Processes ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 126 ◽  
Author(s):  
Antonio Zuorro ◽  
Annalaura Iannone ◽  
Roberto Lavecchia
Keyword(s):  
Antioxidant Activity ◽  
Solvent Extraction ◽  
Organic Solvent ◽  
Animal Feed ◽  
Extraction Procedure ◽  
Extraction Process ◽  
Extraction Yield ◽  
Phenolic Antioxidants ◽  
Brewing Process ◽  
Spent Grain

Brewers’ spent grain (BSG) is the most abundant by-product of the brewing process. BSG is currently disposed of or used as a supplement for animal feed, although it contains significant amounts of bioactive compounds of great interest to the pharmaceutical, cosmetic and food sectors. In this study we investigate the feasibility of using a simple solvent extraction procedure to recover phenolic antioxidants from BSG. Acetone–water and ethanol–water mixtures were used as extraction solvents. Phenolic extracts obtained by treatment of BSG with the two solvent systems were characterized in terms of total phenolics and antioxidant activity. For both systems, the extraction yield was maximum at 60% (v/v) organic solvent concentration. At all solvent compositions, mixtures containing acetone provided higher extraction yields. As suggested by the strong correlation between the antioxidant activity of BSG extracts and their phenolic content, the antioxidant capacity of the extracts can be mainly attributed to polyphenols. Overall, the obtained results strongly support the exploitation of BSG as a source of phenolic antioxidants and the possibility of recovering them by a mild and green extraction process.

scholarly journals The Effect of Cold Plasma Pretreatment on Water-Suspended Herbs Measured in the Content of Bioactive Compounds, Antioxidant Activity, Volatile Compounds and Microbial Count of Final Extracts

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1740
Author(s):  
Ewelina Pogorzelska-Nowicka ◽  
Monika Maria Hanula ◽  
Marta Brodowska-Trębacz ◽  
Elżbieta Górska-Horczyczak ◽  
Urszula Jankiewicz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Plasma Treatment ◽  
Volatile Compounds ◽  
Bioactive Compounds ◽  
Cold Plasma ◽  
New Technology ◽  
Echinacea Purpurea ◽  
Extraction Process ◽  
Extraction Yield ◽  
Microbial Count

Cold plasma is a new technology of promising potential to use as a part of technological extraction lines constructed to implement green chemistry solutions or simply to reduce resources in solvent-based extraction lines. The present study was undertaken to verify the effect of nitrogen cold plasma pre-treatment conducted for 8 min (20 kHz) on the content of antioxidants, antioxidant activity, the profile of volatile compounds, microbial count, pH and color measured in herb extracts (12 herbs: Echinacea purpurea; Salvia officinalis; Urtica dioica; Polygonum aviculare; Vaccinium myrtillus; Taraxacum officinale; Hypericum perforatum; Achillea millefolium; Sanguisorba officinalis; Leonurus cardiaca; Ballota nigra; Andrographis paniculata) obtained with its usage. The surface morphology of extracted herbs was examined as well. Herbs used for extraction were ground and suspended in water before cold plasma treatment, which is a novel approach not studied before. Most plasma-treated extracts were characterized by a higher content of polyphenols (11 out of 12). Content of flavonoids and anthocyanins increased in four extracts and in the case of anthocyanins was significantly higher in comparison to control (up to 77%). The antioxidant activity measured at least by one method (ABTS, DPPH, FRAP) was also higher in nine plasma-treated solutions. Moreover, plasma decreased total aerobic bacteria, affected the color and increased pH of the extracts. The surface structure of the plant material after the extraction process was significantly damaged, which probably led to a higher extraction yield of bioactive compounds and in consequence to the higher antioxidant activity of extracts obtained with the cold plasma treatment.

scholarly journals The Effects of Extraction Conditions on Extraction Yield and Syringin Content in Producing Standardized Tinospora crispa Aqueous Extract with High Antioxidant Activity

2021 ◽  
pp. 106-120
Author(s):  
Zamree Md Shah ◽  
Mohd Kamal Nik Hasan ◽  
Khairul Kamilah Abdul Kadir ◽  
Mohd Shahidan Mohd Arshad ◽  
Zulkhairi Amom
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Pharmaceutical Preparations ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Beneficial Effects ◽  
High Antioxidant Activity ◽  
Optimized Conditions

The herbal plant known as Tinospora crispa is reported to have many beneficial effects on health and has great potential in future to be developed as a health product either in the form of traditional medicine, food supplements or in pharmaceutical preparations. However, so far knowledge on processing procedures to produce quality standardized extracts of this plant to be used in product development has still not widely reported. Therefore, the objective of this study was to determine the optimal extraction conditions for producing a standardized T. crispa aqueos extract (STCAE) with high extraction yield and high syringin content. Experiments were conducted to determine the effects of various extraction conditions involving temperature (25–100 ° C), extraction time (0.5–6 hours) and liquid (water) to solid ratio (5: 1–25: 1 ml of solvent per g stem dry). Using optimized conditions obtained, the extract was standardized based on syringin and was further investigated on its antioxidant activity through DPPH, FRAP and TBA bioassays. Results revealed that the optimum extraction conditions were found to be 1 h extraction time and 15:1 ml g−1 liquid-to-solid ratio. For the extraction temperature, 60°C was found to be the best. STCAE was produced on the basis of the extract to contain with at least 0.4 wt% of syringin. STCAE was found to possess high antioxidant activities through DPPH, FRAP and TBA bioassays.

Effect of Extraction Process on Extraction Yeild, Total Polyphenol Content and Antioxidant Activity of Jasminum Subtriplinerve

2019 ◽  
Vol 814 ◽  
pp. 527-531
Author(s):  
Trieu Tuan Anh ◽  
Luu Tu Hao ◽  
Long Giang Bach ◽  
Duy Chinh Nguyen ◽  
Tri Duc Lam
Keyword(s):  
Antioxidant Activity ◽  
Extraction Process ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Total Phenolic Compounds ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Herbal Plants ◽  
Optimized Conditions

Jasminum Subtriplinerve Blume (Oleaceae) is herbal plants widely a tea for weight loss, stimulates milk glands. Extract product it easy used yet had little research. This study was carried out to evaluate the effect of extracting conditions such as water/material ratio, extraction temperature and extracting time on extracting yield, polyphenol content and antioxidant activity of J.subtriplinerve extract. The total phenolic compounds were determined according to the Folin–Ciocalteu method. Extraction yield (0.22g/100 g) was obtained at water/material ratio 15:1 (ml/g), 40°C and 4 hours extraction time. Under these optimized conditions, the polyphenol content of was 2640.4 μg/1g extract. And the extract can harvest 46.11 % of DPPH (30 μg/ mL) at 10 000 μg/ml concentration

scholarly journals Valorisation of Exhausted Olive Pomace by an Eco-Friendly Solvent Extraction Process of Natural Antioxidants

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1010 ◽  
Author(s):  
Irene Gómez-Cruz ◽  
Cristóbal Cara ◽  
Inmaculada Romero ◽  
Eulogio Castro ◽  
Beatriz Gullón
Keyword(s):  
Bioactive Compounds ◽  
Natural Antioxidants ◽  
Extraction Process ◽  
Industrial Applications ◽  
Antioxidant Compounds ◽  
Olive Pomace ◽  
Operational Parameters ◽  
Food Borne ◽  
High Antioxidant Activity ◽  
Gallic Acid Equivalent

Exhausted olive pomace (EOP) is the waste generated from the drying and subsequent extraction of residual oil from the olive pomace. In this work, the effect of different aqueous solvents on the recovery of antioxidant compounds from this lignocellulosic biomass was assessed. Water extraction was selected as the best option for recovering bioactive compounds from EOP, and the influence of the main operational parameters involved in the extraction was evaluated by response surface methodology. Aqueous extraction of EOP under optimised conditions (10% solids, 85 ºC, and 90 min) yielded an extract with concentrations (per g EOP) of phenolic compounds and flavonoids of 44.5 mg gallic acid equivalent and 114.9 mg rutin equivalent, respectively. Hydroxytyrosol was identified as the major phenolic compound in EOP aqueous extracts. Moreover, these extracts showed high antioxidant activity, as well as moderate bactericidal action against some food-borne pathogens. In general, these results indicate the great potential of EOP as a source of bioactive compounds, with potential uses in several industrial applications.

scholarly journals Optimization of Supercritical Carbon Dioxide and Co-solvent Ethanol Extraction of Wasted Peanut Skin Using Response Surface Methodology

2018 ◽  
Vol 156 ◽  
pp. 02005
Author(s):  
Nicky Rahmana Putra ◽  
Ahmad Hazim Abdul Aziz ◽  
Lee Nian Yian ◽  
Wan Diyana Ramli ◽  
Mohd Azizi Che Yunus
Keyword(s):  
Carbon Dioxide ◽  
Antioxidant Activity ◽  
Supercritical Carbon Dioxide ◽  
Peanut Butter ◽  
Extraction Process ◽  
Nutritional Values ◽  
Box Behnken Design ◽  
Peanut Skin ◽  
High Antioxidant Activity ◽  
Supercritical Carbon

Peanut skin is a waste of peanut industries especially peanut butter industries. Peanut skin contain high antioxidant and high nutritional values. The objective of this study was to optimize the effect of parameters to obtain high extract yield and high antioxidant activity by using supercritical carbon dioxide and co-solvent ethanol. A Box-Behnken design (BBD) was used to optimize the extraction process at the condition temperature (40, 55 and 70 ºC), rate of co-solvent ethanol (2.5, 5, and 7.5 % Vethanol/Vtotal), and extraction pressure (10, 20 and 30 MPa). The extraction time was 3 hours. The optimum condition to obtain yield of extraction and antioxidant activity 22.05 MPa, 62.76 °C and 6.03 %(Vethanol/Vsolvent) with 15.404 % yield extract and 94.040 % antioxidant activity.

scholarly journals Optimization of Extraction Process and the Antioxidant Activity of Phenolics from Sanghuangporus baumii

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3850
Author(s):  
Na Zheng ◽  
Yongfei Ming ◽  
Jianzhi Chu ◽  
Shude Yang ◽  
Guochao Wu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Extraction Process ◽  
Extraction Yield ◽  
Fruiting Bodies ◽  
Factors Affecting ◽  
Medicinal Fungus

Sanghuangporus baumii, is a widely used medicinal fungus. The polyphenols extracted from this fungus exert antioxidant, anti-inflammatory, and hypoglycemic effects. In this study, polyphenols from the fruiting bodies of S. baumii were obtained using the deep eutectic solvent (DES) extraction method. The factors affecting the extraction yield were investigated at different conditions. Based on the results from single-factor experiments, response surface methodology was used to optimize the extraction conditions. The scavenging ability of the polyphenols on •OH, DPPH, and ABTS+ was determined. The results showed that the DES system composed of choline chloride and malic acid had the best extraction yield (6.37 mg/g). The optimal extraction parameters for response surface methodology were as follows: 42 min, 58 ℃, 1:34 solid–liquid (mg/mL), and water content of 39%. Under these conditions, the yield of polyphenols was the highest (12.58 mg/g). At 0.30 mg/mL, the scavenging ability of the polyphenols on •OH, DPPH, and ABTS+ was 95.71%, 91.08%, and 85.52%, respectively. Thus, the method using DES was more effective than the conventional method of extracting phenolic compounds from the fruiting bodies of S. baumii. Moreover, the extracted polyphenols exhibited potent antioxidant activity.

scholarly journals Antioxidant Activities of Muntingia calabura, Syzygium cumini, Ocimum basilicum, and Eleutherine bulbosa using DPPH Method

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Ani Haerani ◽  
Anis Yohana Chaerunisa ◽  
Anas Subarnas
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Extraction Process ◽  
Ocimum Basilicum ◽  
Syzygium Cumini ◽  
Determination Process ◽  
Ic50 Value ◽  
Dpph Method ◽  
Muntingia Calabura

Antioxidants are substances that can provide endogenous protection and exogenous oxidative stress by capturing free radicals. Many plants are efficacious as antioxidants, namely plants that contain polyphenols, especially flavonoids, so many are formulated as natural antioxidants. Plants such as Muntingia calabura, Syzygium cumini, Ocimum basilicum, and Eleutherine bulbosa contain polyphenol compounds, especially flavonoids which are efficacious as natural antioxidants. This research aimed to study antioxidant activity derived from some potential plants using the DPPH method by calculating the IC50 value of each plant extract. This research method starts from the determination process to prove the validity of the plants used, the extraction process using the maceration method with 70% ethanol solvent, then the antioxidant activity of extracts from each plant was carried out using the DPPH method. This research starts from the determination process to ensure the correctness of the plants used, then the extraction process is carried out using the maceration method with 70% ethanol solvent. After that the antioxidant activity was determined from the four plants using the DPPH method to see the strongest IC50 value among the four plants. IC50 is the concentration of the sample to inhibit 50% of free radicals. The results of IC50 values from ethanol extract of M. calabura leaves, Syzygium cumini leaves, Ocimum basilicum leaves and Eleutherine bulbosa bulbs, were 18.72; 63,84; 141.59 and 173.15 ppm. Ethanol extract of M. Calabura has a smaller IC50 value of 18.72 ppm which has a very strong and most powerful antioxidant from the ethanol extract of Syzygium cumini, Ocimum basilicum and Eleutherine bulbosa. Keywords : Antioxidant, Muntingia calabura, Syzygium cumini, Ocimum basilicum, Eleutherine bulbosa, DPPH Method

scholarly journals COMPARATIVE EVALUATION OF THE ANTIOXIDANT POTENTIAL OF Hericium erinaceus, Hericium americanum AND Hericium coralloides

2019 ◽  
Vol 18 (6) ◽  
pp. 97-105
Author(s):  
Funda Atila
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Hericium Erinaceus ◽  
Methanol Extracts ◽  
Ferric Reducing Antioxidant Power ◽  
High Antioxidant Activity

The aim of this work was to determine the total phenolic content (TPC) and the antioxidant activity of methanol extracts of Hericium erinaceus, Hericium americanum and Hericium coralloides, including free radical scavenging method (DPPH), ferric reducing antioxidant power (FRAP) and radical cation scavenging method (ABTS). Hericium spp exhibited moderate to high antioxidant activity. The highest TPC (3.27 ±0.01 mg GAE g–1) and antioxidant activity values (17.0 ±0.68 mmol TE g‒1 in FRAP; EC50 = 4.12 ±0.12 mg mL‒1 in DPPH•; EC50 = 2.83 ± 0.10 mg mL‒1 in ABTS•+) were found for methanol extracts of H. coralloides. The TPC and antioxidant activity of H. erinaceus isolates varied from strain to strain. H. americanum possessed considerably lower total phenolic content (2.31 ±0.01 mg GAE g‒1) and antioxidant activity (10.5 ±0.59 mmol TE g‒1 in FRAP; EC50 = 7.82 ±0.09 mg mL‒1 in DPPH•; EC50 = 6.36 ± 0.12 mg mL‒1 in ABTS•+) than H. coralloides and H. erinaceus. A high correlation was determined between TPC and ABTS•+ (r2 = 0.855), DPPH• (r2 = 0.969) and FRAP (r2 = 0.942). According to results obtained in the present study, Hericium spp., especially H. coralloides and some of H. erinaceus isolates, might be promising natural source of antioxidants for food and pharmaceutical industry.

Extraction of bioactive proteins from seeds (corn, sorghum, and sunflower) and sunflower byproduct: enzymatic hydrolysis and antioxidant properties

2020 ◽  
Vol 16 ◽  
Author(s):  
Danielle Maria Fernandes do Prado ◽  
Adrielle Borges de Almeida ◽  
Josemar Gonçalves Oliveira Filho ◽  
Cassia Cristina Fernandes Alves ◽  
Mariana Buranelo Egea ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Enzymatic Hydrolysis ◽  
Food Systems ◽  
Protein Extraction ◽  
Proteolytic Enzymes ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Extraction Methods ◽  
Degree Of Hydrolysis ◽  
High Antioxidant Activity

Background: Food proteins have benefits for human health, which justifies their production and use. In this context, we highlight the use of seeds and byproduct that would be otherwise discarded to produce protein extracts and hydrolyzed proteins generating opportunities to reduce environmental impacts Objective: This work aimed to use different extraction methods to obtain protein extracts from seeds (corn, sorghum, sunflower) and sunflower byproduct to determine their antioxidant activity, and apply different proteolytic enzymes in the hydrolysis of sunflower byproduct Method: The seeds of corn, sorghum, and sunflower, and sunflower byproduct were ground to produce flour and the protein extracts were prepared using five different methods. The bioactivity of fractions was analyzed by different methods (ABTS, DPPH, and FRAP) to evaluate antioxidant activity Results: The most effective methods, which resulted in higher protein extraction and antioxidant activity, were those in which NH4HCO3 (5 mM, pH 8.0) and H2O/C2H6O (2:3) were used in the extraction. The highest protein contents were 797.9, 303.8, and 11296.5 μg/g, and the highest antioxidant activity was 34417.5, 9732.6, and 47473.1 μg TE/g from Soxhlet and Bligh and Dyer defatted extractions for sunflower seed, and sunflower byproduct, respectively. Regarding enzymatic hydrolysis, sunflower byproduct was the substrate that presented the highest degree of hydrolysis (11.06%) when Neutrase® enzyme was used. Enzymatic hydrolysis increased antioxidant activity in the hydrolyzed proteins, approximately 20.0% using Neutrase® and 22.3% using Flavourzyme® treatments. Conclusion: The protein extracts and the hydrolyzed proteins exhibited high antioxidant activity, demonstrating great potential for use as natural antioxidants in food systems

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