scholarly journals Cellular Factor XIII, a Transglutaminase in Human Corneal Keratocytes

2019 ◽  
Vol 20 (23) ◽  
pp. 5963
Author(s):  
Zsuzsanna Orosz ◽  
Helga Bárdos ◽  
Amir Shemirani ◽  
Ildikó Beke Debreceni ◽  
Riitta Lassila ◽  
...  
Keyword(s):  
Factor Xiii ◽  
Healing Process ◽  
Corneal Stroma ◽  
Cell Types ◽  
Wound Healing Process ◽  
Human Cornea ◽  
Main Function ◽  
Cellular Factor ◽  
Cross Links ◽  
Corneal Keratocytes

Cellular factor XIII (cFXIII, FXIII-A2), a transglutaminase, has been demonstrated in a few cell types. Its main function is to cross-link proteins by isopeptide bonds. Here, we investigated the presence of cFXIII in cells of human cornea. Tissue sections of the cornea were immunostained for FXIII-A in combination with staining for CD34 antigen or isopeptide cross-links. Isolated corneal keratocytes were also evaluated by immunofluorescent microscopy and flow cytometry. FXIII-A in the corneal stroma was quantified by Western blotting. FXIII-A mRNA was detected by RT-qPCR. The cornea of FXIII-A-deficient patients was evaluated by cornea topography. FXIII-A was detected in 68 ± 13% of CD34+ keratocytes. Their distribution in the corneal stroma was unequal; they were most abundant in the subepithelial tertile. cFXIII was of cytoplasmic localization. In the stroma, 3.64 ng cFXIII/mg protein was measured. The synthesis of cFXIII by keratocytes was confirmed by RT-qPCR. Isopeptide cross-links were detected above, but not within the corneal stroma. Slight abnormality of the cornea was detected in six out of nine FXIII-A-deficient patients. The presence of cFXIII in human keratocytes was established for the first time. cFXIII might be involved in maintaining the stability of the cornea and in the corneal wound healing process.

scholarly journals Novel formulation approaches for wound healing

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 937-948
Author(s):  
Tanaji D Nandgude ◽  
Syed Nateque Naser
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Cell Types ◽  
Wound Management ◽  
Wound Healing Process ◽  
Anatomic Structure ◽  
Tissue Integrity ◽  
Molecular Events ◽  
Acute Injuries ◽  
Healing Wounds

A wound is damage to the typical anatomic structure. Wound healing is an immediate therapeutic response to injury. It is a creation of the combined response of some cell types towards injury. Wound healing takes place by a sequence of molecular events which cooperate to fix tissue integrity and cell work. In typical healthy individual under ordinary conditions, these physiological events take place smoothly. Though sometimes, these molecular events are arrested, this brings about in struggle to heal. There is an assortment of approaches for the way toward managing and controlling both acute injuries (acute wounds) and ceaseless non-mending wounds (chronic non-healing wounds). The principal objective of these two cases is to achieve better-wound healing. Ideal formulations of wound healing should not only enhance the healing process but also reduce pain, infection and loss of electrolytes, proteins and liquids from the injury. A broad scope of items typically introduced with target various parts of the wound healing process depends on numerous types of wounds and novel polymers utilised for the conveyance of medications to both acute and ceaseless injuries. These include alginate, hydrocolloids, hydrofibers, polyurethane, and hydrogels. This article gives particular importance to different novel approaches in the management of wound healing. This review draws out the data and hopes to provide understanding into traditional, current and imminent techniques and methods for wound management.

scholarly journals Myocardial infarction remodeling that progresses to heart failure: a signaling misunderstanding

2018 ◽  
Vol 315 (1) ◽  
pp. H71-H79 ◽  
Author(s):  
Alan J. Mouton ◽  
Osvaldo J. Rivera ◽  
Merry L. Lindsey
Keyword(s):  
Myocardial Infarction ◽  
Heart Failure ◽  
Wound Healing ◽  
Current Knowledge ◽  
Healing Process ◽  
Cell Types ◽  
Left Ventricular ◽  
Wound Healing Process ◽  
The Status ◽  
The Right

After myocardial infarction, remodeling of the left ventricle involves a wound-healing orchestra involving a variety of cell types. In order for wound healing to be optimal, appropriate communication must occur; these cells all need to come in at the right time, be activated at the right time in the right amount, and know when to exit at the right time. When this occurs, a new homeostasis is obtained within the infarct, such that infarct scar size and quality are sufficient to maintain left ventricular size and shape. The ideal scenario does not always occur in reality. Often, miscommunication can occur between infarct and remote spaces, across the temporal wound-healing spectrum, and across organs. When miscommunication occurs, adverse remodeling can progress to heart failure. This review discusses current knowledge gaps and recent development of the roles of inflammation and the extracellular matrix in myocardial infarction remodeling. In particular, the macrophage is one cell type that provides direct and indirect regulation of both the inflammatory and scar-forming responses. We summarize current research efforts focused on identifying biomarker indicators that reflect the status of each component of the wound-healing process to better predict outcomes.

COAGULATION FINDINGS IN ULCERATIVE COLITIS AND THE BENEFICIAL EFFECT OF FACTOR XIII CONCENTRATE SUBSTITUTION.

1987 ◽  
Author(s):  
R Suzuki ◽  
Y Takamura
Keyword(s):  
Ulcerative Colitis ◽  
Wound Healing ◽  
Abdominal Pain ◽  
Beneficial Effect ◽  
Factor Xiii ◽  
Healing Process ◽  
Wound Healing Process ◽  
Active Stage ◽  
Abdominal Symptoms ◽  
Mucosal Edema

Reports suggest that there is a tendency towards hypercoagulability in ulcerative colitis (UC). However, few reports deal with factor XIII which plays an important role in the wound healing process.In the present study, 8 coagulation parameters including factor XIII activity were determined in 5 patients with UC at the active stage and in 15 patients at remission. A comparison of the two groups shows that, in the active-stage patients, the levels of factor XIII activity were significantly lower, platelets and fibrinogen higher, and the PT prolonged.Furthermore, Factor XIII concentrate (Fibrogammin P ) was administered to 4 patients with active-stage UC and abdominal symptoms. Here, the symptoms (i.e. abdominal pain, melaena, diarrhea, etc.) disappeared in accordance with the increase in factor XIII activity. Endoscopyprevealed that treatment with factor XIII concentrate (Fibrogammin P ) had a beneficial effect on mucosal edema, redness and hemorrhage as well as on healing of erosions and ulcars.The results suggest that the level of factor XIII activity is a remarkably good measure of the severity of UC and that administration of factor XIII concentrate may be useful for treatment.

Association study between fibronectin and coronary heart disease

2006 ◽  
Vol 44 (1) ◽  
Author(s):  
Ying Zhang ◽  
Xin Zhou ◽  
Joan C. Krepinsky ◽  
Chunhong Wang ◽  
Julien Segbo ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Healing Process ◽  
Positive Association ◽  
Cell Types ◽  
Wound Healing Process ◽  
Plasma Fibronectin ◽  
Chinese Han ◽  
Significant Positive Association ◽  
Extracellular Matrix Components

AbstractFibronectin is a known chemoattractant for several cell types that play a role in the wound healing process, including fibroblasts, endothelial cells and macrophages. It also generates a scaffold that allows attachment of other extracellular matrix components. Large amounts of fibronectin have been detected in atherosclerotic plaques, suggesting that it may play a role in the pathogenesis of atherosclerosis. To examine the possible involvement of fibronectin in the etiology of atherosclerotic coronary heart disease, we analyzed four polymorphisms in the human fibronectin gene and determined the plasma fibronectin levels in patients with coronary heart disease (n=109) and age- and gender-matched controls (n=123) in Chinese Han people. No significant positive association was observed between these polymorphisms and coronary heart disease. The levels of circulating plasma fibronectin, however, were significantly lower in patients with coronary heart disease (mean±SD 245±87mg/L) compared with controls (354±88 mg/L) (p<0.001). The odds ratio (OR) for plasma fibronectin was 0.94 in a multivariate unconditional logistic regression model (OR=0.94, 95% CI 0.91–0.96, p<0.001). We conclude that, in our population, the four fibronectin gene polymorphisms detected are not associated with clinical coronary heart disease. Our data suggest that low circulating fibronectin levels might be a new marker of coronary heart disease.

Topical purified omental lipid formulations in the prevention of skin ulcers: a narrative review

2019 ◽  
Vol 28 (5) ◽  
pp. 284-290 ◽  
Author(s):  
Marco Romanelli ◽  
Valentina Dini ◽  
Massimo Milani
Keyword(s):  
High Risk ◽  
Healing Process ◽  
Wound Healing Process ◽  
Ulcer Formation ◽  
Main Function ◽  
Abdominal Organs ◽  
Skin Ulcers ◽  
Skin Conditions ◽  
Defensive Action ◽  
Endothelial Growth Factor

The omentum is a large peritoneal fold. Its main function is to protect abdominal organs, exerting a defensive action against infective agents. The tissue promotes repair after several types of injury. An extensive vascularisation is the key characteristic of this tissue and the omentum has the highest level of production and content of vascular endothelial growth factor (VEGF). A component of omentum is the lipid compound, which carries out important activities for the organism. Omentum is rich in neutral glycerides, phospholipids, glycolipids and gangliosides. Dermatological products containing purified omental lipids are commercially available and topical omental extracts have been useful in the softening, moisturising and smoothing of skin. Animal-derived omental lipids could be use in topical products with different textures (creams, fluids, emulsions and cleansers) and at different concentrations (10–25%) for the treatment of fragile skin or skin conditions causing risk of ulcer formation. This review summarises the pharmacological rationale of purified omental lipids in topical formulations for use in fragile skin conditions, the clinical efficacy data available in the scientific literature and the potential future perspectives. Efficacy of topical purified omental lipids have been demonstrated in numerous clinical controlled trials involving a total of 320 subjects. These studies demonstrated that this product helps prevent the formation of pressure ulcers (PU) in hospitalised high-risk subjects, improves wound healing process, normalises skin hydration in diabetic subjects with moderate-severe skin xerosis and improve the clinical evolution of diabetic foot. Therefore, purified omental lipid could be an effective tool for the management of fragile skin and the skin at high risk of PU formation.

Adult-Fetal Fibroblast Interactions: Effects on Cell Migration and Implications for Cell Transplantation

2005 ◽  
Vol 14 (5) ◽  
pp. 331-337 ◽  
Author(s):  
Vlad C. Sandulache ◽  
Joseph E. Dohar ◽  
Patricia A. Hebda
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Intrinsic Property ◽  
Cell Types ◽  
Wound Healing Process ◽  
Complex Process ◽  
Culture Model ◽  
Multiple Cell ◽  
Close Cooperation

Wound healing is a complex process involving close cooperation between multiple cell types. During wound healing, fibroblasts are primarily responsible for synthesis of the replacement extracellular matrix. Fibroblast therapy is under investigation in this and other laboratories for its potential use to modulate the final outcome of the wound-healing process. This study addresses the potential interactions between transplanted and host fibroblasts, using a two-dimensional mixed culture model. Our results show that fibroblasts of two different phenotypes, fetal and adult, exhibit different speeds of in vitro migration. These migration speeds are conserved in mixed cocultures, suggesting that the migratory response is an intrinsic property of the fibroblast rather than a response to juxtacrine or paracrine signals. These results have relevance for cell-based therapies in that they demonstrate that donor fibroblasts of a different phenotype may at least partially retain that phenotype in the host environment and in the presence of endogenous fibroblasts.

Preadaptation of cell numbers for wound healing assays

2021 ◽  
Vol 7 (2) ◽  
pp. 295-298
Author(s):  
Jana Markhoff ◽  
Andreas Brietzke ◽  
Niels Grabow
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Cell Density ◽  
Healing Process ◽  
Cell Types ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Specific Cell ◽  
Cell Numbers

Abstract In vitro wound healing assays are a suitable application to verify the efficiency of pharmaceuticals or growth factors that will be incorporated in or immobilized to e.g. electrospun biomaterials for wound dressings or other biological devices in advance. Thereby, various factors like culture conditions or cell density influence the specific cell proliferation. Hence, to establish a wound healing assay for various cell types, a stepwise adaptation of cell numbers was done for better estimation and comparison of cell density for the validation of the influence of drugs on the wound healing process. Cell proliferation of different tissue relevant cell types was evaluated by impedance measurements and live cell imaging. Cell numbers could be successfully adapted for assay specific cell densities. In general, a universal comparison of biological or chemical materials and agents in vitro may require the creation of appropriate ISO or OECD standards for a consistent and cell specific adaptation or demand of initial cell density.

Factor XIII: A Coagulation Factor With Multiple Plasmatic and Cellular Functions

2011 ◽  
Vol 91 (3) ◽  
pp. 931-972 ◽  
Author(s):  
László Muszbek ◽  
Zsuzsanna Bereczky ◽  
Zsuzsa Bagoly ◽  
István Komáromi ◽  
Éva Katona
Keyword(s):  
Factor Xiii ◽  
Bone Development ◽  
Healing Process ◽  
Coagulation Factor ◽  
Fibrin Clot ◽  
Wound Healing Process ◽  
Cellular Functions ◽  
Clotting Factors ◽  
Intracellular Enzyme ◽  
Cell Functions

Factor XIII (FXIII) is unique among clotting factors for a number of reasons: 1) it is a protransglutaminase, which becomes activated in the last stage of coagulation; 2) it works on an insoluble substrate; 3) its potentially active subunit is also present in the cytoplasm of platelets, monocytes, monocyte-derived macrophages, dendritic cells, chondrocytes, osteoblasts, and osteocytes; and 4) in addition to its contribution to hemostasis, it has multiple extra- and intracellular functions. This review gives a general overview on the structure and activation of FXIII as well as on the biochemical function and downregulation of activated FXIII with emphasis on new developments in the last decade. New aspects of the traditional functions of FXIII, stabilization of fibrin clot, and protection of fibrin against fibrinolysis are summarized. The role of FXIII in maintaining pregnancy, its contribution to the wound healing process, and its proangiogenic function are reviewed in details. Special attention is given to new, less explored, but promising fields of FXIII research that include inhibition of vascular permeability, cardioprotection, and its role in cartilage and bone development. FXIII is also considered as an intracellular enzyme; a separate section is devoted to its intracellular activation, intracellular action, and involvement in platelet, monocyte/macrophage, and dendritic cell functions.

HUBUNGAN MOBILISASI DINI TERHADAP PENYEMBUHAN LUKA POST OPERASI SECTIO CESARIA

2019 ◽  
Vol 2 (1) ◽  
pp. 14-17
Author(s):  
Nurul Aini Siagian ◽  
Syafira Nusaibah ◽  
Andayani Boang Manalu
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Early Mobilization ◽  
Sectio Caesarea ◽  
Wound Healing Process ◽  
P Value ◽  
Cross Sectional ◽  
Care Services ◽  
Healing Wound ◽  
Rapid Healing

Early mobilization includes factors that can affect the process of wound healing after surgery. Immediate mobilization in stages is very useful for the process of healing wounds and preventing infection and venous thrombosis. The purpose of this study was to determine whether there is a relationship between early mobilization and the process of healing wound post operative sectio caesarea at Sinar Husni General Hospital Medan Helvetia. The research design used was analytic survey with cross sectional approach. The sample in this study used the Consecutive Sampling method of data collection using a checklist sheet conducted on a sample of 19 respondents. The results of the study in this study are the majority of respondents who did early mobilization and who experienced rapid wound healing process as many as 4 people (21%) while the minority of respondents who did early mobilization and who experienced slow wound healing process were 1 person (5.3%). The majority of respondents who did not mobilize early and who experienced a slow wound healing process were 11 people (57.9%) and a minority who did not mobilize early and who experienced rapid healing as many as 4 people (21.1%). Statistical test results obtained p value = 0.046 <0.005. The conclusions of the results of this study indicate there is a relationship between early mobilization and the process of healing post operative sectio of caesarea. Suggestions The results of this study can be applied as a reference to improve nursing care services, especially in providing counseling and assistance to patients.

Hydrogels: A Versatile Drug Delivery Carrier Systems

1970 ◽  
Vol 5 (3) ◽  
pp. 1745-1756
Author(s):  
L H Baldaniya ◽  
Sarkhejiya N A
Keyword(s):  
Drug Delivery ◽  
Protein Delivery ◽  
Structural Integrity ◽  
Healing Process ◽  
Polymer Chains ◽  
Wound Healing Process ◽  
Aqueous Environment ◽  
Preparation Methods ◽  
Control Drug

Hydrogels are the material of choice for many applications in regenerative medicine due to their unique properties including biocompatibility, flexible methods of synthesis, range of constituents, and desirable physical characteristics. Hydrogel (also called Aquagel) is a network of polymer chains that are hydrophilic, sometimes found as a colloidal gel in which water is the dispersion medium. Hydrogels are highly absorbent (contain ~99.9% water), natural or synthetic polymers. Hydrogel also possess a degree of flexibility very similar to natural tissue, due to its significant water content. It can serve as scaffolds that provide structural integrity to tissue constructs, control drug and protein delivery to tissues and cultures. Also serve as adhesives or barriers between tissue and material surfaces. The positive effect of hydrogels on wounds and enhanced wound healing process has been proven. Hydrogels provide a warm, moist environment for wound that makes it heal faster in addition to its useful mucoadhesive properties. Moreover, hydrogels can be used as carriers for liposomes containing variety of drugs, such as antimicrobial drugs. Hydrogels are water swollen polymer matrices, with a tendency to imbibe water when placed in aqueous environment. This ability to swell, under biological conditions, makes it an ideal material for use in drug delivery and immobilization of proteins, peptides, and other biological compounds. Hydrogels have been extensively investigated for use as constructs to engineer tissues in vitro. This review describes the properties, classification, preparation methods, applications, various monomer used in formulation and development of hydrogel products.

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