scholarly journals Different MicroRNA Families Involved in Regulating High Temperature Stress Response during Cotton (Gossypium hirsutum L.) Anther Development

2020 ◽  
Vol 21 (4) ◽  
pp. 1280
Author(s):  
Jin Chen ◽  
Ao Pan ◽  
Shujun He ◽  
Pin Su ◽  
Xiaoling Yuan ◽  
...  
Keyword(s):  
High Temperature ◽  
Stress Response ◽  
Target Genes ◽  
Developmental Stages ◽  
High Temperature Stress ◽  
Small Rna Sequencing ◽  
Plant Tolerance ◽  
Gossypium Hirsutum L ◽  
Response Characteristics ◽  
Expression Abundance

MicroRNAs (miRNAs) are small molecule RNAs widely involved in responses to plant abiotic stresses. We performed small RNA sequencing of cotton anthers at four developmental stages under normal and high temperature (NT and HT, respectively) conditions to investigate the stress response characteristics of miRNA to HT. A total of 77 miRNAs, including 33 known miRNAs and 44 novel miRNAs, were identified, and 41 and 28 miRNAs were differentially expressed under NT and HT stress conditions, respectively. The sporogenous cell proliferation (SCP), meiotic phase (MP), microspore release period (MRP), and pollen maturity (PM) stages had 10 (including 12 miRNAs), four (including six miRNAs), four (including five miRNAs), and seven (including 11 miRNAs) HT stress-responsive miRNA families, respectively, which were identified after removing the changes in genotype-specific miRNAs under NT condition. Seven miRNA families (miR2949, miR167, and miR160 at the SCP stage; miR156 and miR172 at the MP stage; miR156 at the MRP stage; and miR393 and miR3476 at the PM stage), which had expression abundance of more than 10% of the total expression abundance, served as the main regulators responding to HT stress with positive or negative regulation patterns. These miRNAs orchestrated the expression of the corresponding target genes and led to different responses in the HT-tolerant and the HT-sensitive lines. The results revealed that the HT stress response of miRNAs in cotton anthers were stage-specific and differed with the development of anthers. Our study may enhance the understanding of the response of miRNAs to HT stress in cotton anthers and may clarify the mechanism of plant tolerance to HT stress.

High temperature stress response is not sexually dimorphic at the whole-body level and is dependent on androgens to induce sex reversal

2020 ◽  
Vol 299 ◽  
pp. 113605
Author(s):  
Diana C. Castañeda-Cortés ◽  
Jing Zhang ◽  
Agustín F. Boan ◽  
Valerie S. Langlois ◽  
Juan I. Fernandino
Keyword(s):  
High Temperature ◽  
Stress Response ◽  
Temperature Stress ◽  
High Temperature Stress ◽  
Sex Reversal ◽  
Whole Body ◽  
Sexually Dimorphic ◽  
Body Level

scholarly journals Construction and Characterization of aHelicobacter pylori clpB Mutant and Role of the Gene in the Stress Response

1998 ◽  
Vol 180 (2) ◽  
pp. 426-429 ◽  
Author(s):  
Elaine Allan ◽  
Peter Mullany ◽  
Soad Tabaqchali
Keyword(s):  
Helicobacter Pylori ◽  
High Temperature ◽  
Stress Response ◽  
Structural Features ◽  
High Temperature Stress ◽  
Gene Encoding ◽  
Increased Sensitivity ◽  
H Pylori

ABSTRACT Antiserum raised against whole Helicobacter pyloricells identified a novel 94-kDa antigen. The nucleotide sequence of the gene encoding the 94-kDa antigen was determined, and analysis of the deduced amino acid sequence revealed structural features typical of the ClpB ATPase family of stress response proteins. An isogenic H. pylori clpB mutant showed increased sensitivity to high-temperature stress, indicating that the clpB gene product functions as a stress response protein in H. pylori.

scholarly journals High-Temperature-Responsive Poplar lncRNAs Modulate Target Gene Expression via RNA Interference and Act as RNA Scaffolds to Enhance Heat Tolerance

2020 ◽  
Vol 21 (18) ◽  
pp. 6808
Author(s):  
Yuepeng Song ◽  
Panfei Chen ◽  
Peng Liu ◽  
Chenhao Bu ◽  
Deqiang Zhang
Keyword(s):  
High Temperature ◽  
Rna Interference ◽  
Stress Responses ◽  
Target Gene ◽  
Target Genes ◽  
Calcium Influx ◽  
High Temperature Stress ◽  
High Temperature Treatment ◽  
Ecological Stability ◽  
Temperature Responsive

High-temperature stress is a threat to plant development and survival. Long noncoding RNAs (lncRNAs) participate in plant stress responses, but their functions in the complex stress response network remain unknown. Poplar contributes to terrestrial ecological stability. In this study, we identified 204 high-temperature-responsive lncRNAs in an abiotic stress-tolerant poplar (Populus simonii) species using strand-specific RNA sequencing (ssRNA-seq). Mimicking overexpressed and repressed candidate lncRNAs in poplar was used to illuminate their regulation pattern on targets using nano sheet mediation. These lncRNAs were predicted to target 185 genes, of which 100 were cis genes and 119 were trans genes. Gene Ontology enrichment analysis showed that anatomical structure morphogenesis and response to stress and signaling were significantly enriched. Among heat-responsive LncRNAs, TCONS_00202587 binds to upstream sequences via its secondary structure and interferes with target gene transcription. TCONS_00260893 enhances calcium influx in response to high-temperature treatment by interfering with a specific variant/isoform of the target gene. Heterogeneous expression of these two lncRNA targets promoted photosynthetic protection and recovery, inhibited membrane peroxidation, and suppressed DNA damage in Arabidopsis under heat stress. These results showed that lncRNAs can regulate their target genes by acting as potential RNA scaffolds or through the RNA interference pathway.

scholarly journals A Systematic View Exploring the Role of Chloroplasts in Plant Abiotic Stress Responses

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Yo-Han Yoo ◽  
Woo-Jong Hong ◽  
Ki-Hong Jung
Keyword(s):  
Abiotic Stress ◽  
High Temperature ◽  
Stress Response ◽  
Temperature Stress ◽  
Stress Responses ◽  
Cellular Response ◽  
Molecular Mechanisms ◽  
Close Association ◽  
High Temperature Stress ◽  
Growth And Yield

Chloroplasts are intracellular semiautonomous organelles central to photosynthesis and are essential for plant growth and yield. The significance of the function of chloroplast-related genes in response to climate change has not been well studied in crops. In the present study, the initial focus was on genes that were predicted to be located in the chloroplast genome in rice, a model crop plant, with genes either preferentially expressed in the leaf or ubiquitously expressed in all organs. The characteristics were analyzed by Gene Ontology (GO) enrichment and MapMan functional classification tools. It was then identified that 110 GO terms (45 for leaf expression and 65 for ubiquitous expression) and 1,695 genes mapped to MapMan overviews were strongly associated with chloroplasts. In particular, the MapMan cellular response overview revealed a close association between heat stress response and chloroplast-related genes in rice. Moreover, features of these genes in response to abiotic stress were analyzed using a large-scale publicly available transcript dataset. Consequently, the expression of 215 genes was found to be upregulated in response to high temperature stress. Conversely, genes that responded to other stresses were extremely limited. In other words, chloroplast-related genes were found to affect abiotic stress response mainly through high temperature response, with little effect on response to drought and salinity stress. These results suggest that genes involved in diurnal rhythm in the leaves participate in the reaction to recognize temperature changes in the environment. Furthermore, the predicted protein–protein interaction network analysis associated with high temperature stress is expected to provide a very important basis for the study of molecular mechanisms by which chloroplasts will respond to future climate changes.

scholarly journals Overexpression of OsPT8 Increases Auxin Content and Enhances Tolerance to High-Temperature Stress in Nicotiana tabacum

Genes ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 809 ◽  
Author(s):  
Song ◽  
Fan ◽  
Jiao ◽  
Liu ◽  
Wang ◽  
...  
Keyword(s):  
High Temperature ◽  
Temperature Stress ◽  
Root Architecture ◽  
Molecular Mechanisms ◽  
Extreme Temperature ◽  
High Temperature Stress ◽  
Wild Plants ◽  
Auxin Signaling ◽  
Plant Tolerance ◽  
Auxin Content

Temperature is a primary factor affecting the rate of plant development; as the climate warms, extreme temperature events are likely to increasingly affect agriculture. Understanding how to improve crop tolerance to heat stress is a key concern. Wild plants have evolved numerous strategies to tolerate environmental conditions, notably the regulation of root architecture by phytohormones, but the molecular mechanisms of stress resistance are unclear. In this study, we showed that high temperatures could significantly reduce tobacco biomass and change its root architecture, probably through changes in auxin content and distribution. Overexpression of the OsPT8 phosphate transporter enhanced tobacco tolerance to high-temperature stress by changing the root architecture and increased the antioxidant ability. Molecular assays suggested that overexpression of OsPT8 in tobacco significantly increased the expression of auxin synthesis genes NtYUCCA 6, 8 and auxin efflux carriers genes NtPIN 1,2 under high-temperature stress. We also found that the expression levels of auxin response factors NtARF1 and NtARF2 were increased in OsPT8 transgenic tobacco under high-temperature stress, suggesting that OsPT8 regulates auxin signaling in response to high-temperature conditions. Our findings provided new insights into the molecular mechanisms of plant stress signaling and showed that OsPT8 plays a key role in regulating plant tolerance to stress conditions.

scholarly journals WRKY Transcription Factor Response to High-Temperature Stress

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2211
Author(s):  
Zhuoya Cheng ◽  
Yuting Luan ◽  
Jiasong Meng ◽  
Jing Sun ◽  
Jun Tao ◽  
...  
Keyword(s):  
High Temperature ◽  
Plant Growth ◽  
Signaling Pathways ◽  
Temperature Stress ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
High Temperature Stress ◽  
Research Progress ◽  
Plant Responses ◽  
Gene Promoters

Plant growth and development are closely related to the environment, and high-temperature stress is an important environmental factor that affects these processes. WRKY transcription factors (TFs) play important roles in plant responses to high-temperature stress. WRKY TFs can bind to the W-box cis-acting elements of target gene promoters, thereby regulating the expression of multiple types of target genes and participating in multiple signaling pathways in plants. A number of studies have shown the important biological functions and working mechanisms of WRKY TFs in plant responses to high temperature. However, there are few reviews that summarize the research progress on this topic. To fully understand the role of WRKY TFs in the response to high temperature, this paper reviews the structure and regulatory mechanism of WRKY TFs, as well as the related signaling pathways that regulate plant growth under high-temperature stress, which have been described in recent years, and this paper provides references for the further exploration of the molecular mechanisms underlying plant tolerance to high temperature.

scholarly journals Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis

2009 ◽  
Vol 7 (1) ◽  
pp. 33 ◽  
Author(s):  
Apiradee Hongsthong ◽  
Matura Sirijuntarut ◽  
Rayakorn Yutthanasirikul ◽  
Jittisak Senachak ◽  
Pavinee Kurdrid ◽  
...  
Keyword(s):  
High Temperature ◽  
Stress Response ◽  
Temperature Stress ◽  
Spirulina Platensis ◽  
High Temperature Stress
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