scholarly journals Integrative Analysis of Transcriptome and Metabolome Reveals Salt Stress Orchestrating the Accumulation of Specialized Metabolites in Lycium barbarum L. Fruit

2021 ◽  
Vol 22 (9) ◽  
pp. 4414
Author(s):  
Shuang Lin ◽  
Shaohua Zeng ◽  
Biao A ◽  
Xiaoman Yang ◽  
Tianshun Yang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Pearson Correlation ◽  
Structural Genes ◽  
Lycium Barbarum ◽  
Specialized Metabolites ◽  
Yield And Quality ◽  
Solid Foundation ◽  
Pearson Correlation Analysis ◽  
Stress Influences

Salt stress seriously affects yield and quality of crops. The fruit of Lycium barbarum (LBF) is extensively used as functional food due to its rich nutrient components. It remains unclear how salt stress influences the quality of LBF. In this study, we identified 71 differentially accumulated metabolites (DAMs) and 1396 differentially expressed genes (DEGs) among ripe LBF with and without 300 mM of NaCl treatment. Pearson correlation analysis indicated that the metabolomic changes caused by salt stress were strongly related to oxidoreductases; hydrolases; and modifying enzymes, in particular, acyltransferases, methyltransferases and glycosyltransferases. Further analysis revealed that salt stress facilitated flavonoid glycosylation and carotenoid esterification by boosting the expression of structural genes in the biosynthetic pathways. These results suggested that salt stress prompts the modification of flavonoids and carotenoids to alleviate ROS damage, which in turn improves the quality of LBF. Our results lay a solid foundation for uncovering the underlying molecular mechanism of salt stress orchestrating LBF quality, and the candidate genes identified will be a valuable gene resource for genetic improvement of L. barbarum.

scholarly journals Matrix management interfers in yield and quality of cocoa seedlings

2018 ◽  
Vol 40 (3) ◽  
Author(s):  
Rafaela Alves Pereira ◽  
Gedeon Almeida Gomes Júnior ◽  
Eliandro Malta Rodrigues ◽  
George Andrade Sodré ◽  
Célio Kersul do Sacramento
Keyword(s):  
Quality Index ◽  
Quality Evaluation ◽  
Pearson Correlation ◽  
Dry Mass ◽  
Experimental Unit ◽  
Plant Management ◽  
Yield And Quality ◽  
Cocoa Seedlings ◽  
Dickson Quality Index

Abstract The influence of two managements on yield and quality of plagiotropic mini-cuttings of four cacao clones was evaluated. The design was completely randomized in a 4 x 2 factorial scheme with 4 clones x 2 mother plant management (apical pruning and bending), with 10 replicates and one plant as experimental unit. Seedlings used were propagated by rooting of semi-woody cuttings of plagiotropic branches and at seven months of age, two seedlings of each clone were transplanted to 12 L pots filled with organomineral substrate. After transplantation, the apex of one of the plants was cut based on the count of the first six leaves from the apex. For bending, stems were folded and horizontally fixed. The first collection of mini-cuttings was performed 30 days after transplantation and the others during the following seven months. The yield of mini-cuttings was estimated during collections and part was used for rooting and quality evaluation of seedlings. After 90 days, 10 mini-cuttings of each clone were evaluated for quality using the Dickson quality index (DQI). Another 10 rooted mini-cuttings were transplanted to 1.5 L polyethylene bags and kept in greenhouse for another 120 days until final quality evaluation at 210 days. Data were submitted to analysis of variance and Tukey test at 5% probability. Shapiro-Wilk normality test and Pearson correlation analysis were performed. bending was more efficient in the average production of mini-cuttings especially for BN 34 clone. The Dickson quality index was correlated with root dry mass, at both 90 and 210 days, and the bending technique was responsible for the best DQI. Variable height was not adequate to estimate the quality of cocoa seedlings.

scholarly journals RsSOS1 Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na+ Homeostasis in Radish (Raphanus sativus L.)

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 458
Author(s):  
Wanting Zhang ◽  
Jingxue Li ◽  
Junhui Dong ◽  
Yan Wang ◽  
Liang Xu ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Functional Characterization ◽  
Vital Role ◽  
Salt Stress Response ◽  
Reading Frame ◽  
Yield And Quality

Radish is a kind of moderately salt-sensitive vegetable. Salt stress seriously decreases the yield and quality of radish. The plasma membrane Na+/H+ antiporter protein Salt Overly Sensitive 1 (SOS1) plays a crucial role in protecting plant cells against salt stress, but the biological function of the RsSOS1 gene in radish remains to be elucidated. In this study, the RsSOS1 gene was isolated from radish genotype ‘NAU-TR17’, and contains an open reading frame of 3414 bp encoding 1137 amino acids. Phylogenetic analysis showed that RsSOS1 had a high homology with BnSOS1, and clustered together with Arabidopsis plasma membrane Na+/H+ antiporter (AtNHX7). The result of subcellular localization indicated that the RsSOS1 was localized in the plasma membrane. Furthermore, RsSOS1 was strongly induced in roots of radish under 150 mmol/L NaCl treatment, and its expression level in salt-tolerant genotypes was significantly higher than that in salt-sensitive ones. In addition, overexpression of RsSOS1 in Arabidopsis could significantly improve the salt tolerance of transgenic plants. Meanwhile, the transformation of RsSOS1△999 could rescue Na+ efflux function of AXT3 yeast. In summary, the plasma membrane Na+/H+ antiporter RsSOS1 plays a vital role in regulating salt-tolerance of radish by controlling Na+ homeostasis. These results provided useful information for further functional characterization of RsSOS1 and facilitate clarifying the molecular mechanism underlying salt stress response in radish.

scholarly journals The Influence of the Service Quality of Eyelash Beauty Treatment and Perceived Value on Behavior Intention

2021 ◽  
Vol 27 (6) ◽  
pp. 1407-1416
Author(s):  
Soo-Ji Kim ◽  
Hye-Soon Heo ◽  
Mi-Ock Woo
Keyword(s):  
Regression Analysis ◽  
Data Analysis ◽  
Service Quality ◽  
Pearson Correlation ◽  
Perceived Value ◽  
Hierarchical Regression ◽  
Behavior Intention ◽  
Pearson Correlation Analysis ◽  
Reliability Verification

In this study, a questionnaire survey was conducted with women aged 20 to 50, in order to find how the service quality of eyelash beauty treatment and perceived value influence their behavior intention. For data analysis, SPSS23.0 and JAMOVI 1.6.16 program were used. For reliability verification, Cronbach’s α value was analyzed, and Pearson correlation analysis and hierarchical regression analysis were conducted. Firstly, according to the analysis on the influence of service quality on perceived value, assurance and empathy as the factors of service quality positively and significantly influenced perceived value. Secondly, according to the analysis on the influence of service quality and perceived value on behavior intention, assurance positively and significantly influenced behavior intention; responsiveness negatively and significantly influenced behavior intention. Therefore, The more assurance in perceived value and service quality, and the lower responsiveness in service quality, the more behavior intention.

scholarly journals Factors Affecting Quality of Life of Hemodialysis Patients - with Focus on Resilience, Symptom Experience and Self-management

2021 ◽  
Vol 46 (4) ◽  
pp. 429-434
Author(s):  
Hyun-Young Jung ◽  
Yong-Kyung Park ◽  
Soon-Rim Suh
Keyword(s):  
Quality Of Life ◽  
Pearson Correlation ◽  
Nursing Intervention ◽  
Hemodialysis Patients ◽  
Symptom Experience ◽  
Factors Affecting ◽  
Medical Institutions ◽  
Pearson Correlation Analysis ◽  
Influential Variable

Objectives: The purpose of this study was to investigate the factors affecting quality of life of hemodialysis patients.Methods: As a descriptive study, the data were collected from 172 hemodialysis patients receiving hemodialysis at 4 medical institutions. Collected data were analyzed using descriptive statistics, t-test, ANOVA, Pearson correlation analysis and multiple regression.Results: The influential variable of the quality of Life of hemodialysis patients were resilience, symptom experience and monthly income less than 2 million won. These factors explained for 48.7% of the quality of Life of hemodialysis patients.Conclusions: The most ideal method to increase the quality of hemodialysis patients’ lives is to develop an integrated nursing intervention that will increase patients’ resilience and reduce the intensity of symptoms.

scholarly journals Analysis of the Sagittal Balance in Patients with Adult Spinal Deformity: Modified Sagittal Vertical Axis

2021 ◽  
Author(s):  
Yang Yu ◽  
Bing Wu ◽  
Zhaohan Wang ◽  
Junyao Cheng ◽  
Bo Li ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Orthopedic Surgery ◽  
Sagittal Balance ◽  
Adult Spinal Deformity ◽  
Pearson Correlation ◽  
Vertical Axis ◽  
Sagittal Vertical Axis ◽  
Life Score ◽  
Pearson Correlation Analysis

Abstract Objective: The overall sagittal balance of patient with adult spinal deformity (ASD) is crucial for satisfactory postoperative outcome. SVA is the parameter often used to assess the sagittal balance. However, pelvis often rotates backward to compensate for sagittal imbalance in patients with ASD. In the case, SVA cannot reflect the real sagittal balance. Modified sagittal vertical axis (MSVA) is the parameter we found to better assess the real sagittal balance in patients with ASD. And we want to explore the relationship between MSVA and the quality of life. Methods: We used sample of 60 patients with ASD who underwent long-segment orthopedic surgery (≥4 vertebrae) between the period of 2015 and 2018. The paired-sample t test and Pearson correlation analysis were used for statistical analysis. P value <0.05 was considered significant. Results: SVA, TK, TLK, LL, SS, PT, PI-LL and MSVA were significantly changed in 60 patients preoperatively and postoperatively (p<0.05). The ODI, SRS22, VAS scores were significantly improved postoperatively (p<0.05). Pearson correlation analysis was used to conclude that postoperative SVA, postoperative MSVA and postoperative quality of life score were related. Postoperative MSVA has the strongest correlation with the quality-of-life score. Conclusions: Through spinal orthopedic surgery, the postoperative spinal parameters and quality of life of patients with ASD were significantly improved. MSVA is of great significance for evaluating postoperative sagittal balance and quality of life of patients with ASD.

scholarly journals Stability of extruded diets for dogs

2020 ◽  
Vol 19 (3) ◽  
pp. 236-242
Author(s):  
Daniele Cristina de Lima ◽  
Tais Silvino Bastos ◽  
Camilla Mariane Menezes Souza ◽  
Juarez Ribeiro da Silva ◽  
Simone Gisele de Oliveira ◽  
...  
Keyword(s):  
Shelf Life ◽  
Crude Protein ◽  
Pearson Correlation ◽  
Ether Extract ◽  
Pearson Correlation Analysis ◽  
Dry Food ◽  
Positive Correlations ◽  
The Stability ◽  
The Relationship

Controlling the factors that influence the conservation of extruded dog foods can increase shelf life and/or guarantee the quality of shelf life of diets for these animals. Therefore, the objective was to evaluate the relationship among water activity (Aw), moisture (M), acidity, lipid peroxidation, crude protein (CP), ether extract (EE), and kibble size of extruded dog foods stored in sealed and open packages for 60 days (Experiment I). We also evaluated the stability of the Aw for up to 6.5 hours after coating with palatant (Experiment II). We manufactured four extruded dry dog foods: high CP and EE (HPE); low CP and EE (LPE); small kibble (SK); and large kibble (LK). In experiment I, the foods were stored in sealed 1 kg packages and 10 kg open packages, for a period of 60 days. We measured Aw, M, acidity, peroxide, CP, and EE of foods and the relative humidity (RH). Data were subjected to Pearson correlation analysis. For experiment II, samples were collected immediately after coating with palatant. Sub samples were collected every half hour to measure Aw. In experiment I, positive correlations were observed (P0.05) among kibble size, M, and Aw; acidity, CP, EE, and M; and between Aw and RH, for open and sealed packages. There was also a positive correlation (P0.05) for open packages among time, Aw, and peroxide. In experiment II, LK and LPE food presented Aw stabilized in less time. Diets with higher kibble size and high CP and EE levels are more unstable if kept in open packages. Extruded dry food with higher protein and lipid and smaller kibble size needs more time to stabilize its Aw.

scholarly journals Integrated Transcriptome and Proteome Analysis Reveals Complex Regulatory Mechanism of Cotton in Response to Salt Stress

2020 ◽  
Author(s):  
Lin Chen ◽  
Heng Sun ◽  
Jie Kong ◽  
Haijiang Xu ◽  
Xiyan Yang
Keyword(s):  
Salt Stress ◽  
Proteome Analysis ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Complex Signal ◽  
Calcium Signal ◽  
Signal Pathways ◽  
Yield And Quality ◽  
Signal Cascade

Abstract BackgroundSoil salt stress seriously restricts the yield and quality of cotton worldwide. To investigate the molecular mechanism of cotton response to salt stress, a main cultivated variety Gossypium hirsutum L. acc. Xinluzhong 54 was used to perform transcriptome and proteome integrated analysis. ResultsThrough transcriptome analysis of cotton treated with salt stress for 0 h (T0), 3 h (T3) and 12 h (T12), we identified 8,436, 11,628 and 6,311 differentially expressed genes (DEGs) inT3 / T0, T12 / T0 and T12 / T3, respectively. A total of 459 differentially expressed proteins (DEPs) were identified by proteomic analysis, of which 273, 99 and 260 DEPs were identified in T3 / T0, T12 / T0 and T12 / T3, respectively. Metabolic pathways, biosynthesis of secondary metabolites, photosynthesis and plant hormone signal transduction were the main enrichment pathways by annotation of DEGs or DEPs. Detail analysis of the DEGs or DEPs revealed that complex signal pathways, such as ABA and JA signal, calcium signal, MAPK signal cascade, transcription factors, followed by activation of antioxidant and ion transporters, were identified to participate in regulating salt response in cotton.ConclusionsOur results not only contribute to understand the mechanism of cotton response to salt stress, but also provide nine candidate genes, which might be used for molecular breeding to improve salt-tolerance in cotton.

scholarly journals Pazarsuyu Deresi (Giresun, Türkiye) Sediment Kalitesinin Çok Değişkenli İstatistik Yöntemlerle Belirlenmesi

2018 ◽  
Vol 6 (3) ◽  
pp. 304 ◽  
Author(s):  
Fikret Ustaoğlu ◽  
Yalçın Tepe
Keyword(s):  
Heavy Metal ◽  
Pearson Correlation ◽  
Sediment Quality ◽  
Quality Criteria ◽  
Black Sea Region ◽  
Metal Levels ◽  
Statistical Program ◽  
Pearson Correlation Analysis ◽  
The City

The present study, aimed to determine sediment quality of Pazarsuyu Stream, located in the city limit Giresun, Black Sea Region, were carried out between June 2014 and May 2015. The average heavy metal levels of samples taken seasonally from four selected stations were as follow; Cr; 10.64 ppm, Mn; 155.83 ppm, Fe; 8312 ppm, Co; 5.73 ppm, Cu; 17.79 ppm, Zn; 32.74 ppm, Cd; 0.16 ppm, Pb; 19.69 ppm. Additionally, the average sediment organic matter % and pH were found as 6.64 and 5.02, respectively. The obtained data were evaluated statistically by one way ANOVA, Pearson correlation analysis, cluster, and factor analysis. Dot graphs were made by using PAST statistical program. The level of heavy metal pollution in the stream sediment were determined by comparisons of our graphs with sediment quality criteria and the mean heavy metal concentrations of the earth.

scholarly journals Impact of Nitrogen Fertilizer Levels on Metabolite Profiling of the Lycium barbarum L. Fruit

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3879 ◽  
Author(s):  
Zhigang Shi ◽  
Feng Wei ◽  
Ru Wan ◽  
Yunxiang Li ◽  
Yajun Wang ◽  
...  
Keyword(s):  
Organic Acids ◽  
Nitrogen Fertilizer ◽  
Molecular Mechanisms ◽  
Ultra Performance Liquid Chromatography ◽  
Lycium Barbarum ◽  
Nitrogen Levels ◽  
Goji Berry ◽  
Yield And Quality ◽  
Significant Difference

The yield and quality of goji (Lycium barbarum L.) fruit are heavily dependent on fertilizer, especially the availability of nitrogen, phosphorus, and potassium (N, P, and K, respectively). In this study, we performed a metabolomic analysis of the response of goji berry to nitrogen fertilizer levels using an Ultra Performance Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry (UPLC-ESI-MS/MS) method. There was no significant difference in the fruit yield or the commodity grade between N0 (42.5 g/plant), N1 (85 g/plant), and N2 (127.5 g/plant). The primary nutrients of the goji berry changed with an increasing nitrogen fertilization. Comparative metabolomic profiling of three nitrogen levels resulted in the identification of 612 metabolites, including amino acids, flavonoids, carbohydrates, organic acids, and lipids/alcohols, among others, of which 53 metabolites (lipids, fatty acids, organic acids, and phenolamides) demonstrated significant changes. These results provide new insights into the molecular mechanisms of the relationship between yield and quality of goji berry and nitrogen fertilizer.

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