scholarly journals Antimicrobial Properties of Palladium and Platinum Nanoparticles: A New Tool for Combating Food-Borne Pathogens

2021 ◽  
Vol 22 (15) ◽  
pp. 7892
Author(s):  
Ondrej Chlumsky ◽  
Sabina Purkrtova ◽  
Hana Michova ◽  
Hana Sykorova ◽  
Petr Slepicka ◽  
...  
Keyword(s):  
Metallic Nanoparticles ◽  
Food Packaging ◽  
Antimicrobial Properties ◽  
In Vitro Cytotoxicity ◽  
Dermal Fibroblasts ◽  
Bacterial Cells ◽  
Human Coronary Artery ◽  
Food Borne Pathogens ◽  
Food Borne

Although some metallic nanoparticles (NPs) are commonly used in the food processing plants as nanomaterials for food packaging, or as coatings on the food handling equipment, little is known about antimicrobial properties of palladium (PdNPs) and platinum (PtNPs) nanoparticles and their potential use in the food industry. In this study, common food-borne pathogens Salmonella enterica Infantis, Escherichia coli, Listeria monocytogenes and Staphylococcus aureus were tested. Both NPs reduced viable cells with the log10 CFU reduction of 0.3–2.4 (PdNPs) and 0.8–2.0 (PtNPs), average inhibitory rates of 55.2–99% for PdNPs and of 83.8–99% for PtNPs. However, both NPs seemed to be less effective for biofilm formation and its reduction. The most effective concentrations were evaluated to be 22.25–44.5 mg/L for PdNPs and 50.5–101 mg/L for PtNPs. Furthermore, the interactions of tested NPs with bacterial cell were visualized by transmission electron microscopy (TEM). TEM visualization confirmed that NPs entered bacteria and caused direct damage of the cell walls, which resulted in bacterial disruption. The in vitro cytotoxicity of individual NPs was determined in primary human renal tubular epithelial cells (HRTECs), human keratinocytes (HaCat), human dermal fibroblasts (HDFs), human epithelial kidney cells (HEK 293), and primary human coronary artery endothelial cells (HCAECs). Due to their antimicrobial properties on bacterial cells and no acute cytotoxicity, both types of NPs could potentially fight food-borne pathogens.

scholarly journals Nanovesicles Loaded with Origanum onites and Satureja thymbra Essential Oils and Their Activity against Food-Borne Pathogens and Spoilage Microorganisms

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2124
Author(s):  
Giulia Vanti ◽  
Ekaterina-Michaela Tomou ◽  
Dejan Stojković ◽  
Ana Ćirić ◽  
Anna Rita Bilia ◽  
...  
Keyword(s):  
Essential Oils ◽  
Propylene Glycol ◽  
Antimicrobial Properties ◽  
Spherical Shape ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Spoilage Microorganisms ◽  
Satureja Thymbra ◽  
Origanum Onites

Food poisoning is a common cause of illness and death in developing countries. Essential oils (EOs) could be effective and safe natural preservatives to prevent and control bacterial contamination of foods. However, their high sensitivity and strong flavor limit their application and biological effectiveness. The aim of this study was firstly the chemical analysis and the antimicrobial evaluation of the EOs of Origanum onites L. and Satureja thymbra L. obtained from Symi island (Greece), and, secondly, the formulation of propylene glycol-nanovesicles loaded with these EOs to improve their antimicrobial properties. The EOs were analyzed by GC-MS and their chemical contents are presented herein. Different nanovesicles were formulated with small average sizes, high homogeneity, and optimal ζ-potential. Microscopic observation confirmed their small and spherical shape. Antibacterial and antifungal activities of the formulated EOs were evaluated against food-borne pathogens and spoilage microorganisms compared to pure EOs. Propylene glycol-nanovesicles loaded with O. onites EO were found to be the most active formulation against all tested strains. Additionally, in vitro studies on the HaCaT cell line showed that nanovesicles encapsulated with EOs had no toxic effect. The present study revealed that both EOs can be used as alternative sanitizers and preservatives in the food industry, and that their formulation in nanovesicles can provide a suitable approach as food-grade delivery system.

scholarly journals Evaluation of the Antimicrobial Efficacy of N-Acetyl-L-Cysteine, Rhamnolipids, and Usnic Acid—Novel Approaches to Fight Food-Borne Pathogens

2021 ◽  
Vol 22 (21) ◽  
pp. 11307
Author(s):  
Ondrej Chlumsky ◽  
Heidi J. Smith ◽  
Albert E. Parker ◽  
Kristen Brileya ◽  
James N. Wilking ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Biofilm Formation ◽  
Usnic Acid ◽  
Food Contamination ◽  
In Vitro Cytotoxicity ◽  
Confocal Scanning Laser Microscopy ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
The Impact

In the food industry, the increasing antimicrobial resistance of food-borne pathogens to conventional sanitizers poses the risk of food contamination and a decrease in product quality and safety. Therefore, we explored alternative antimicrobials N-acetyl-L-cysteine (NAC), rhamnolipids (RLs), and usnic acid (UA) as a novel approach to prevent biofilm formation and reduce existing biofilms formed by important food-borne pathogens (three strains of Salmonella enterica and two strains of Escherichia coli, Listeria monocytogenes, Staphylococcus aureus). Their effectiveness was evaluated by determining minimum inhibitory concentrations needed for inhibition of bacterial growth, biofilm formation, metabolic activity, and biofilm reduction. Transmission electron microscopy and confocal scanning laser microscopy followed by image analysis were used to visualize and quantify the impact of tested substances on both planktonic and biofilm-associated cells. The in vitro cytotoxicity of the substances was determined as a half-maximal inhibitory concentration in five different cell lines. The results indicate relatively low cytotoxic effects of NAC in comparison to RLs and UA. In addition, NAC inhibited bacterial growth for all strains, while RLs showed overall lower inhibition and UA inhibited only the growth of Gram-positive bacteria. Even though tested substances did not remove the biofilms, NAC represents a promising tool in biofilm prevention.

scholarly journals Essential oil of the leaves of Ricinus communis L.: In vitro cytotoxicity and antimicrobial properties

2012 ◽  
Vol 11 (1) ◽  
pp. 102 ◽  
Author(s):  
Zied Zarai ◽  
Ines Chobba ◽  
Riadh Mansour ◽  
Ahmed Békir ◽  
Néji Gharsallah ◽  
...  
Keyword(s):  
Essential Oil ◽  
Ricinus Communis ◽  
Antimicrobial Properties ◽  
In Vitro Cytotoxicity ◽  
Ricinus Communis L

Emerging silver nanomaterials for smart food packaging in combating food-borne pathogens

2021 ◽  
pp. 147-185
Author(s):  
Divya Sachdev ◽  
Akanksha Joshi ◽  
Neetu Kumra Taneja ◽  
Renu Pasricha
Keyword(s):  
Food Packaging ◽  
Silver Nanomaterials ◽  
Food Borne Pathogens ◽  
Food Borne

scholarly journals Cytotoxic T-Lymphocyte–Defined Human Minor Histocompatibility Antigens With a Restricted Tissue Distribution

Blood ◽  
1998 ◽  
Vol 91 (6) ◽  
pp. 2197-2207 ◽  
Author(s):  
Edus H. Warren ◽  
Philip D. Greenberg ◽  
Stanley R. Riddell
Keyword(s):  
Hematopoietic Cells ◽  
In Vitro Cytotoxicity ◽  
Dermal Fibroblasts ◽  
Leukemic Cells ◽  
Transplant Proc ◽  
Cellular Immunotherapy ◽  
Target Cells ◽  
Allogeneic Bone ◽  
Allogeneic Bmt

Abstract Cytotoxic T lymphocytes (CTL) specific for human minor histocompatibility (H) antigens can be isolated from the blood of major histocompatibility complex (MHC)-matched allogeneic bone marrow transplant (BMT) recipients and may play a prominent role in the graft-versus-host (GVH) and graft-versus-leukemia (GVL) reactions (Tsoi et al, J Immunol 125:2258, 1980; Tsoi et al, Transplant Proc 15:1484, 1983; Goulmy et al, Nature 302:159, 1983; Irle et al, Transplantation 40:329, 1985; and Niederwieser et al, Blood 81:2200, 1993). The identification of minor H antigens that are expressed in hematopoietic cells, including leukemic cells, but not in fibroblasts and other tissue types has suggested that such tissue-restricted antigens could potentially serve as targets for T-cell immunotherapy to enhance GVL activity without inducing GVH disease (de Bueger et al, J Immunol 149:1788, 1992; van der Harst et al, Blood 83:1060, 1994; and Dolstra et al, J Immunol 158:560, 1997). To explore the feasibility of this strategy, donor CD3+CD8+ CTL clones specific for recipient minor H antigens were isolated and characterized from allogeneic BMT recipients. CTL clones were obtained from the majority of donor/recipient pairs. Seventeen distinct minor H antigens distinguishable by their MHC-restricting allele, population frequency, and/or distribution of tissue expression were defined by 56 CD3+CD8+ CTL clones isolated from these patients. The MHC-restricting alleles for these CTL clones included HLA-A2 and HLA-B7, which had previously been shown to present minor H antigens to CTL, as well as HLA-A3, -A11, -B8, -B53, and -Cw7, which had not previously been described to present minor H antigens to CTL. Estimated phenotype frequencies for these 17 distinct minor H antigens range from 0.17 to 0.92. In vitro cytotoxicity assays using hematopoietic cells and fibroblasts as target cells showed that 5 of the 17 minor H antigens were expressed in both hematopoietic cells and fibroblasts. However, 12 were presented for CTL recognition only by hematopoietic cells and not by dermal fibroblasts derived from the same donors. These results significantly extend the spectrum of CTL-defined human minor H antigens that could potentially serve as target antigens for cellular immunotherapy to promote GVL activity after allogeneic BMT.

scholarly journals Preliminary data on the antimicrobial effect of Cannabis sativa L. variety Futura 75 against food-borne pathogens in vitro as well as against naturally occurring microbial populations on minced meat during storage

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Frédérique Pasquali ◽  
Marco Schinzari ◽  
Alex Lucchi ◽  
Mara Mandrioli ◽  
Tullia Gallina Toschi ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Naturally Occurring ◽  
Food Borne ◽  
Minced Beef ◽  
Microbial Groups ◽  
Staphylococcus Spp ◽  
Minced Meat

In the present study, the antimicrobial effect of Cannabis sativa Futura 75 was evaluated both in vitro against foodborne bacterial pathogens, and on food against naturally occurring microbial groups of minced meat stored for 8 days at 4°C. Ethanol extraction was performed on the grind of the inflorescence. After extraction, ethanol was completely evaporated and substituted by water. Serial dilutions of the extract, the grind and cannabidiol 99% were added to Nutrient Agar and spotted with Listeria monocytogenes, Salmonella Typhimurium, Escherichia coli and Staphylococcus spp. Regarding the evaluation on food, 50 mL of extract, characterised by CBD at concentration of 322,70 μg/mL, were added to 2.5 kg of minced beef meat. Meat was divided into aliquots and stored for 8 days at 4°C. At 0, 1, 2, 3, 4, 7, and 8 days, aerobic bacteria, enterobacteria, coliforms and E. coli were enumerated. All tested products were efficient against Gram +. In particular, extract corresponding to CBD concentration of 0.017 and 0.3 mg/mL were effective against L. monocytogenes and Staphylococcus spp. respectively. After 8 days of storage at 4°C, treated minced meat showed a bright red colour in comparison to a brownish control meat. Moreover, Enterobacteriaceae and coliforms were significantly reduced of 2.3 log CFU/g and 1.6 log CFU/g respectively in treated meat in comparison to the control. Although preliminary, the present study suggests the antimicrobial properties of the extract of Cannabis sativa both in vitro and in minced meat.

Colletotrichum sp.- mediated synthesis of sulphur and aluminium oxide nanoparticles and its in vitro activity against selected food-borne pathogens

LWT ◽  
2017 ◽  
Vol 81 ◽  
pp. 188-194 ◽  
Author(s):  
Pooja Suryavanshi ◽  
Raksha Pandit ◽  
Aniket Gade ◽  
Marcos Derita ◽  
Susana Zachino ◽  
...  
Keyword(s):  
Aluminium Oxide ◽  
Vitro Activity ◽  
Oxide Nanoparticles ◽  
In Vitro Activity ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Aluminium Oxide Nanoparticles

In vitro antimicrobial activity of Muntingia calabura fruit extracts against food borne pathogens

2013 ◽  
Vol 5 (3) ◽  
pp. 135-136 ◽  
Author(s):  
G. Sibi ◽  
Kalpana Kaushik ◽  
Kalegowda Dhananjaya ◽  
Kodagahally R. Ravikumar ◽  
Honnaiah Mallesha
Keyword(s):  
Antimicrobial Activity ◽  
In Vitro Antimicrobial Activity ◽  
Fruit Extracts ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Muntingia Calabura

scholarly journals Comparison of antibacterial effect of seven 1-monoglycerides on food-borne pathogens or spoilage bacteria

2011 ◽  
Vol 80 (1) ◽  
pp. 29-39 ◽  
Author(s):  
Leona Buňková ◽  
František Buňka ◽  
Rahula Janiš ◽  
Jiří Krejčí ◽  
Iva Doležálková ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Growth Rate ◽  
Specific Growth ◽  
Lag Time ◽  
Gram Negative Bacteria ◽  
Inhibitory Effects ◽  
Gram Negative ◽  
Food Borne Pathogens ◽  
Food Borne

The aim of this study was to compare under the same conditionsin vitrothe inhibitory effects of seven 1-monoglycerides (MAG) containing fatty acids with a medium chain on ten strains of food-borne pathogens or spoilage gram-positive and gram-negative bacteria (Bacillus cereus, Bacillus subtilis, Enterococcus faecalis, Micrococcus luteus, Staphylococcus aureus, Citrobacter freundii, Escherichia coli, Proteus mirabilis, Salmonella entericaser.Enteritidis andPseudomonas aeruginosa) and on their growth indicatos. The inhibitory effect of MAGs (monocaprylin, monocaprin, monolaurin, monomyristin, monopalmitin, MAG of undecanoic and 10-undecenoic acids) at a concentration of 25 – 1500 mg·l-1was observed. Growth of bacteria in the presence of MAG was studied by means of optical density of bacteria for 24 h. The data were modelled through a Gompertz equation and the lag-time, the maximum specific growth rate and the maximal value reached were calculated. MAGs inhibited mainly the growth of gram-positive bacteria, which was shown by the extended lag-time, decrease in specific growth rate and decrease in cell density. Inhibitory effects of tested MAGs could be ranked from point of view of the minimum inhibitory concentration: MAG-C12:0 > MAG-C11:0 > MAG-C10:0 > MAG-C14:0 > MAG-C11:1 > MAG-C8:0 > MAG-C16:0.In vitro, no significant inhibitory effects of 1-monoglycerides, with the exception of the highest concentrations applied, on the growth of gram-negative bacteria were detected. The main contribution of this study is to compare the effects of several MAG containing fatty acids with a medium chain under the same conditions on the growth indicators of bacteria.

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