Hydrogen Sulfide Improves the Cold Stress Resistance through the CsARF5-CsDREB3 Module in Cucumber

As an important gas signaling molecule, hydrogen sulfide (H2S) plays a crucial role in regulating cold tolerance. H2S cooperates with phytohormones such as abscisic acid, ethylene, and salicylic acid to regulate the plant stress response. However, the synergistic regulation of H2S and auxin in the plant response to cold stress has not been reported. This study showed that sodium hydrosulfide (NaHS, an H2S donor) treatment enhanced the cold stress tolerance of cucumber seedlings and increased the level of auxin. CsARF5, a cucumber auxin response factor (ARF) gene, was isolated, and its role in regulating H2S-mediated cold stress tolerance was described. Transgenic cucumber leaves overexpressing CsARF5 were obtained. Physiological analysis indicated that overexpression of CsARF5 enhanced the cold stress tolerance of cucumber and the regulation of the cold stress response by CsARF5 depends on H2S. In addition, molecular assays showed that CsARF5 modulated cold stress response by directly activating the expression of the dehydration-responsive element-binding (DREB)/C-repeat binding factor (CBF) gene CsDREB3, which was identified as a positive regulator of cold stress. Taken together, the above results suggest that CsARF5 plays an important role in H2S-mediated cold stress in cucumber. These results shed light on the molecular mechanism by which H2S regulates cold stress response by mediating auxin signaling; this will provide insights for further studies on the molecular mechanism by which H2S regulates cold stress. The aim of this study was to explore the molecular mechanism of H2S regulating cold tolerance of cucumber seedlings and provide a theoretical basis for the further study of cucumber cultivation and environmental adaptability technology in winter.

Download Full-text

Hydrogen sulfide improves the cold stress resistance through the CsARF5-CsDREB3 module in cucumber

10.1101/2021.09.18.460893 ◽

2021 ◽

Author(s):

Xiao-Wei Zhang ◽

Xin Fu ◽

Feng-Jiao Liu ◽

Ya-Nan Wang ◽

Huan-Gai Bi ◽

...

Keyword(s):

Hydrogen Sulfide ◽

Stress Response ◽

Cold Stress ◽

Stress Tolerance ◽

Cold Tolerance ◽

Molecular Mechanism ◽

Auxin Signaling ◽

Sodium Hydrosulfide ◽

Physiological Analysis ◽

Responsive Element

AbstractHydrogen sulfide (H2S) plays a crucial role in regulating cold tolerance. But the synergistic regulation of H2S and auxin in the plant response to cold stress has not been reported. In the study, we found that sodium hydrosulfide (NaHS, an H2S donor) treatment enhanced the cold tolerance of cucumber seedlings and increased the level of auxin. CsARF5, a cucumber auxin response factor (ARF) gene was isolated and its role in regulating H2S-mediated cold stress tolerance was described. Transgenic cucumber leaves overexpressing CsARF5 were obtained. Physiological analysis indicated that overexpression of CsARF5 enhanced the cold stress tolerance of cucumber and the regulation of the cold stress response by CsARF5 depends on H2S. In addition, molecular assays showed that CsARF5 modulated cold stress response by directly activating the expression of the dehydration-responsive element-binding (DREB)/C-repeat binding factor (CBF) gene CsDREB3, which was identified as a positive regulator of cold stress. Taken together, our results suggest that CsARF5 plays an important role in H2S-mediated cold stress in cucumber. These results shed light on the molecular mechanism by which H2S regulates cold stress response by mediating auxin signaling, and will provide insights for further studies on the molecular mechanism by which H2S regulates cold stress.HighlightAuxin signaling participates in H2S-mediated cold stress through the CsARF5-CsDREB3 module in cucumber.

Download Full-text

GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response

Plant Physiology ◽

10.1093/plphys/kiab142 ◽

2021 ◽

Author(s):

Zemin Wang ◽

Darren Chern Jan Wong ◽

Yi Wang ◽

Guangzhao Xu ◽

Chong Ren ◽

...

Keyword(s):

Stress Response ◽

Cold Stress ◽

Cold Tolerance ◽

Cold Treatment ◽

Ectopic Expression ◽

Bimolecular Fluorescence Complementation ◽

Luciferase Reporter ◽

Vitis Amurensis ◽

Mobility Shift

Abstract Cultivated grapevine (Vitis) is a highly valued horticultural crop, and cold stress affects its growth and productivity. Wild Amur grape (Vitis amurensis) PAT1 (Phytochrome A signal transduction 1, VaPAT1) is induced by low temperature, and ectopic expression of VaPAT1 enhances cold tolerance in Arabidopsis (Arabidopsis thaliana). However, little is known about the molecular mechanism of VaPAT1 during the cold stress response in grapevine. Here, we confirmed the overexpression of VaPAT1 in transformed grape calli enhanced cold tolerance. Yeast two-hybrid and bimolecular fluorescence complementation assays highlighted an interaction between VaPAT1 with INDETERMINATE-DOMAIN 3 (VaIDD3). A role of VaIDD3 in cold tolerance was also indicated. Transcriptome analysis revealed VaPAT1 and VaIDD3 overexpression and cold treatment coordinately modulate the expression of stress-related genes including lipoxygenase 3 (LOX3), a gene encoding a key jasmonate biosynthesis enzyme. Co-expression network analysis indicated LOX3 might be a downstream target of VaPAT1. Both electrophoretic mobility shift and dual luciferase reporter assays showed the VaPAT1-IDD3 complex binds to the IDD-box (AGACAAA) in the VaLOX3 promoter to activate its expression. Overexpression of both VaPAT1 and VaIDD3 increased the transcription of VaLOX3 and JA levels in transgenic grape calli. Conversely, VaPAT1-SRDX (dominant repression) and CRISPR/Cas9-mediated mutagenesis of PAT1-ED causing the loss of the C-terminus in grape calli dramatically prohibited the accumulation of VaLOX3 and JA levels during cold treatment. Together, these findings point to a pivotal role of VaPAT1 in the cold stress response in grape by regulating JA biosynthesis.

Download Full-text

Phylogenetics of Molecular Regulators Contributing to Plant Stress Tolerance

Diversity ◽

10.3390/d12100407 ◽

2020 ◽

Vol 12 (10) ◽

pp. 407

Author(s):

Xiang Yu ◽

Yan Bao

Keyword(s):

Stress Response ◽

Plant Breeding ◽

Stress Tolerance ◽

Wild Species ◽

Plant Stress ◽

Gene Families ◽

Homologous Gene ◽

Plant Tolerance ◽

Functional Conservation ◽

Plant Stress Tolerance

Genetic studies on model plants and crops in the last few decades have uncovered numerous genes that play vital roles in plant tolerance to adverse environments. These genes could be used as targets for genetic engineering to improve plant tolerance to abiotic and biotic stresses. Recent advances in CRISPR-based genome editing have accelerated modern plant breeding and wild-species domestication. However, the stress regulators in many crops and horticultural cultivars and their wild species remain largely unexplored. Thus, transferring the accumulated knowledge of these molecular regulators from model plants to a wider range of other species is critical for modern plant breeding. Phylogenetic analysis is one of the powerful strategies for studying the functional conservation and diversity of homologous gene families among different species with complete genome sequences available. In addition, many transcriptome datasets of plants under stress conditions have been publicly released, providing a useful resource for addressing the stress response of given gene families. This Special Issue aims to illustrate the phylogenetics of molecular regulators with potential in contributing to plant stress tolerance and their stress response diversity in multiple non-model plants.

Download Full-text

Glyoxalases and stress tolerance in plants

Biochemical Society Transactions ◽

10.1042/bst20130242 ◽

2014 ◽

Vol 42 (2) ◽

pp. 485-490 ◽

Cited By ~ 72

Author(s):

Charanpreet Kaur ◽

Ajit Ghosh ◽

Ashwani Pareek ◽

Sudhir K. Sopory ◽

Sneh L. Singla-Pareek

Keyword(s):

Stress Response ◽

Stress Tolerance ◽

Present Article ◽

Environmental Conditions ◽

Multigene Family ◽

Abiotic Stresses ◽

Plant Stress ◽

Stress Conditions ◽

Plant Stress Tolerance ◽

Adverse Environmental Conditions

The glyoxalase pathway is required for detoxification of cytotoxic metabolite MG (methylglyoxal) that would otherwise increase to lethal concentrations under adverse environmental conditions. Since its discovery 100 years ago, several roles have been assigned to glyoxalases, but, in plants, their involvement in stress response and tolerance is the most widely accepted role. The plant glyoxalases have emerged as multigene family and this expansion is considered to be important from the perspective of maintaining a robust defence machinery in these sessile species. Glyoxalases are known to be differentially regulated under stress conditions and their overexpression in plants confers tolerance to multiple abiotic stresses. In the present article, we review the importance of glyoxalases in plants, discussing possible roles with emphasis on involvement of the glyoxalase pathway in plant stress tolerance.

Download Full-text

Small heat shock protein Hsp67Bc plays a significant role in Drosophila melanogaster cold stress tolerance

Journal of Experimental Biology ◽

10.1242/jeb.219592 ◽

2020 ◽

Vol 223 (21) ◽

pp. jeb219592

Author(s):

Dina Malkeyeva ◽

Elena Kiseleva ◽

Svetlana Fedorova

Keyword(s):

Drosophila Melanogaster ◽

Stress Response ◽

Heat Shock ◽

Heat Shock Protein ◽

Cold Stress ◽

Stress Tolerance ◽

Developmental Stages ◽

Small Heat Shock Protein ◽

Recovery Phase ◽

Main Function

ABSTRACTHsp67Bc in Drosophila melanogaster is a member of the small heat shock protein family, the main function of which is to prevent the aggregation of misfolded or damaged proteins. Hsp67Bc interacts with Starvin and Hsp23, which are known to be a part of the cold stress response in the fly during the recovery phase. In this study, we investigated the role of the Hsp67Bc gene in the cold stress response. We showed that in adult Drosophila, Hsp67Bc expression increases after cold stress and decreases after 1.5 h of recovery, indicating the involvement of Hsp67Bc in short-term stress recovery. We also implemented a deletion in the D. melanogaster Hsp67Bc gene using imprecise excision of a P-element, and analysed the cold tolerance of Hsp67Bc-null mutants at different developmental stages. We found that Hsp67Bc-null homozygous flies are viable and fertile but display varying cold stress tolerance throughout the stages of ontogenesis: the survival after cold stress is slightly impaired in late third instar larvae, unaffected in pupae, and notably affected in adult females. Moreover, the recovery from chill coma is delayed in Hsp67Bc-null adults of both sexes. In addition, the deletion in the Hsp67Bc gene caused more prominent up-regulation of Hsp70 following cold stress, suggesting the involvement of Hsp70 in compensation of the lack of the Hsp67Bc protein. Taken together, our results suggest that Hsp67Bc is involved in the recovery of flies from a comatose state and contributes to the protection of the fruit fly from cold stress.

Download Full-text

Hydrogen sulfide alleviates chlorobenzene toxicity in soybean seedlings

Ciência Rural ◽

10.1590/0103-8478cr20151503 ◽

2016 ◽

Vol 46 (10) ◽

pp. 1743-1749 ◽

Cited By ~ 1

Author(s):

Jin Feng Chen ◽

Chan Jiao

Keyword(s):

Hydrogen Sulfide ◽

Plant Stress ◽

Root Growth Inhibition ◽

Oxygen Species ◽

Cell Toxicity ◽

Sodium Hydrosulfide ◽

Soybean Seedlings ◽

Cysteine Desulfhydrase ◽

Pre Treatment

ABSTRACT: As a gas signaling molecule, endogenous hydrogen sulfide (H2S) plays a crucial role in the plant stress response. However, the role of H2S in the response to organic pollutants specifically has not been studied. Here, the effects of H2S addition on soybean (Glycine max) seedlings tolerance of 1,4-dichlorobenzene (1,4-DCB) were investigated. Under 1,4-DCB stress, the growth of soybean seedlings roots and stems was inhibited, while L-/D-cysteine desulfhydrase (LCD/DCD) activity was induced and endogenous H2S increased. When applied jointly with sodium hydrosulfide (NaHS), a H2S donor, root growth inhibition was effectively alleviated. Pre-treatment of seedlings with 0.4mmol L-1 NaHS reduced the malondialdehyde (MDA) and reactived oxygen species (ROS) content, mitigating root cell toxicity significantly. Further experiments confirmed that NaHS enhanced soybean seedlings peroxidase (POD) and superoxide dismutase (SOD) enzyme activities. In contrast, these effects were reversed by hypotaurine (HT), a H2S scavenger. Therefore, H2S alleviated 1,4-DCB toxicity in soybean seedlings by regulating antioxidant enzyme activity to reduce cell oxidative damage.

Download Full-text

OsCBE1, a Substrate Receptor of Cullin4-Based E3 Ubiquitin Ligase, Functions as a Regulator of Abiotic Stress Response and Productivity in Rice

International Journal of Molecular Sciences ◽

10.3390/ijms22052487 ◽

2021 ◽

Vol 22 (5) ◽

pp. 2487

Author(s):

Juyoung Choi ◽

Wonkyung Lee ◽

Gynheung An ◽

Seong-Ryong Kim

Keyword(s):

Abiotic Stress ◽

Stress Response ◽

Cold Stress ◽

Stress Tolerance ◽

Ubiquitin Ligase ◽

E3 Ubiquitin Ligase ◽

In Silico Analysis ◽

Wild Type ◽

Abiotic Stress Response ◽

Environmental Stress Response

Ubiquitination is an important environmental stress response, and E3 ubiquitin ligases play a major role in the process. T-DNA insertion mutants of rice, Oscbe1-1, and Oscbe1-2, were identified through the screening of cold stress tolerance at seedling stage. Oscbe1 mutants showed a significantly higher cold stress tolerance in the fresh weight, chlorophyll content, and photosynthetic efficiency than wild type. Molecular prediction showed that OsCBE1 (Oryza sativa Cullin4-Based E3 ubiquitin ligase1) encoded a novel substrate receptor of Cullin4-based E3 ubiquitin ligase complex (C4E3). Whereas Oscbe1 mutants had fewer panicles and grains than wild type in the paddy field, the overexpression lines of OsCBE1 had more panicles and grains, suggesting that OsCBE1 is involved in the regulation of both abiotic stress response and development. Oscbe1 mutants also showed ABA hypersensitivity during seed germination, suggesting OsCBE1 function for the stress response via ABA signaling. In silico analysis of OsCBE1 activity predicted a CCCH-type transcription factor, OsC3H32, as a putative substrate. Co-IP (Co-immunoprecipitation) study showed that OsCBE1 interacts with OsDDB1, an expected binding component of OsCBE1 and OsC3H32. Additionally, expression of OsOLE16, OsOLE18, and OsBURP5 were negatively related with expression of OsCBE1. These results suggest that OsCBE1 functions as a regulator of the abiotic stress response via CCCH as a member of the C4E3.

Download Full-text

Citrus sinensis CBF1 Functions in Cold Tolerance by Modulating Putrescine Biosynthesis Through Regulation of ARGININE DECARBOXYLASE

Plant and Cell Physiology ◽

10.1093/pcp/pcab135 ◽

2021 ◽

Author(s):

Jie Song ◽

Hao Wu ◽

Feng He ◽

Jing Qu ◽

Yue Wang ◽

...

Keyword(s):

Transcription Factors ◽

Stress Response ◽

Transgenic Plants ◽

Cold Stress ◽

Cold Tolerance ◽

Citrus Sinensis ◽

Sweet Orange ◽

Arginine Decarboxylase ◽

Wild Type ◽

Shed Light

Abstract C-repeat (CRT) binding factors (CBFs) are well known to act as crucial transcription factors that function in cold stress response. Arginine decarboxylase (ADC)-mediated putrescine biosynthesis has been reported to be activated in plants exposed to cold conditions, but it remains elusive whether CBFs can regulate ADC expression and putrescine accumulation. In this study, we show that cold up-regulated ADC gene (CsADC) and elevation of endogenous putrescine content in sweet orange (Citrus sinensis). Promoter of CsADC contains two CRT sequences that are canonical elements recognized by CBFs. Sweet orange genome contains four CBFs (CsCBF1-4), in which CsCBF1 was significantly induced by cold. CsCBF1, located in the nucleus, was demonstrated to bind directly and specifically to the promoter of CsADC and acted as a transcriptional activator. Overexpression of CsCBF1 led to notable elevation of CsADC and putrescine level in sweet orange transgenic plants, along with remarkably enhanced cold tolerance, relative to the wild type (WT). However, pretreatment with D-arginine, an ADC inhibitor, caused prominent reduction of endogenous putrescine level in the overexpressing lines, accompanied by greatly compromised cold tolerance. Taken together, these results demonstrate that CBF1 of sweet orange directly regulates ADC expression and modulates putrescine synthesis for orchestrating the cold tolerance. Our findings shed light into the transcriptional regulation of putrescine accumulation through targeting the ADC gene in the presence of cold stress. Meanwhile, this study illustrates a new mechanism underlying the CBF-mediated cold stress response.

Download Full-text

Quantitative Proteomic Analysis of Castor (Ricinus communis L.) Seeds During Early Imbibition Provided Novel Insights into Cold Stress Response

International Journal of Molecular Sciences ◽

10.3390/ijms20020355 ◽

2019 ◽

Vol 20 (2) ◽

pp. 355 ◽

Cited By ~ 3

Author(s):

Xiaoyu Wang ◽

Min Li ◽

Xuming Liu ◽

Lixue Zhang ◽

Qiong Duan ◽

...

Keyword(s):

Fatty Acid ◽

Stress Response ◽

Cold Stress ◽

Cold Tolerance ◽

Stress Responses ◽

Fatty Acid Biosynthesis ◽

Ricinus Communis ◽

Seedling Emergence ◽

Proteomics Data ◽

Castor Seeds

Early planting is one of the strategies used to increase grain yield in temperate regions. However, poor cold tolerance in castor inhibits seed germination, resulting in lower seedling emergence and biomass. Here, the elite castor variety Tongbi 5 was used to identify the differential abundance protein species (DAPS) between cold stress (4 °C) and control conditions (30 °C) imbibed seeds. As a result, 127 DAPS were identified according to isobaric tag for relative and absolute quantification (iTRAQ) strategy. These DAPS were mainly involved in carbohydrate and energy metabolism, translation and posttranslational modification, stress response, lipid transport and metabolism, and signal transduction. Enzyme-linked immunosorbent assays (ELISA) demonstrated that the quantitative proteomics data collected here were reliable. This study provided some invaluable insights into the cold stress responses of early imbibed castor seeds: (1) up-accumulation of all DAPS involved in translation might confer cold tolerance by promoting protein synthesis; (2) stress-related proteins probably protect the cell against damage caused by cold stress; (3) up-accumulation of key DAPS associated with fatty acid biosynthesis might facilitate resistance or adaptation of imbibed castor seeds to cold stress by the increased content of unsaturated fatty acid (UFA). The data has been deposited to the ProteomeXchange with identifier PXD010043.

Download Full-text