Cross-Talk between Probiotic Nissle 1917 and Human Colonic Epithelium Affects the Metabolite Composition and Demonstrates Host Antibacterial Effect

Colonic epithelium–commensal interactions play a very important role in human health and disease development. Colonic mucus serves as an ecologic niche for a myriad of commensals and provides a physical barrier between the epithelium and luminal content, suggesting that communication between the host and microbes occurs mainly by soluble factors. However, the composition of epithelia-derived metabolites and how the commensal flora influences them is less characterized. Here, we used mucus-producing human adult stem cell-derived colonoid monolayers exposed apically to probiotic E. coli strain Nissle 1917 to characterize the host–microbial communication via small molecules. We measured the metabolites in the media from host and bacterial monocultures and from bacteria-colonoid co-cultures. We found that colonoids secrete amino acids, organic acids, nucleosides, and polyamines, apically and basolaterally. The metabolites from host-bacteria co-cultures markedly differ from those of host cells grown alone or bacteria grown alone. Nissle 1917 affects the composition of apical and basolateral metabolites. Importantly, spermine, secreted apically by colonoids, shows antibacterial properties, and inhibits the growth of several bacterial strains. Our data demonstrate the existence of a cross-talk between luminal bacteria and human intestinal epithelium via metabolites, which might affect the numbers of physiologic processes including the composition of commensal flora via bactericidal effects.

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Triterpenoid Acids and Lactones from the Leaves of Fadogia tetraquetra var. tetraquetra (Rubiaceae)

Natural Product Communications ◽

10.1177/1934578x1100601103 ◽

2011 ◽

Vol 6 (11) ◽

pp. 1934578X1100601 ◽

Cited By ~ 1

Author(s):

Dulcie A. Mulholland ◽

Abdelhafeez M.A. Mohammed ◽

Philip H. Coombes ◽

Shafiul Haque ◽

Leena L. Pohjala ◽

...

Keyword(s):

Escherichia Coli ◽

Hepatocellular Carcinoma ◽

Semliki Forest Virus ◽

Antibacterial Properties ◽

Enterobacter Aerogenes ◽

Host Cells ◽

Baby Hamster Kidney ◽

Atp Content ◽

Bacterial Strains ◽

Viability Assay

Four triterpenoids isolated from the leaves of Fadogia tetraquetra var. tetraquetra, 3β-hydroxy-11α,12α-epoxyoleanan-28,13β-olide (1), 3β-hydroxyurs-11-en-28,13β-olide (2), oleanolic acid (3), and ursolic acid (4), were evaluated for their antiviral and antibacterial properties. Compound 4 showed potent activity against the Semliki Forest virus with an IC50 of 14.7 μM, but was also found to be significantly cytotoxic (68% reduction in cell viability after 24 hours exposure at 50 μM) towards baby hamster kidney (BHK21) host cells. A viability assay on the mammalian human hepatocellular carcinoma (Huh-7) cell line showed no significant effects on intracellular ATP content after 48 hours exposure to compounds 1-4 at this concentration. Compound 4 also inhibited Staphylococcus aureus (MIC 12.5 μM), but was inactive against Enterobacter aerogenes, Escherichia coli, and Pseudomonas aeruginosa. Compounds 1-3 were inactive against all tested bacterial strains at 50 μM concentration.

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Antibacterial Activities of Soaps Formulated from Carapa procera Oil

Microbiology Research Journal International ◽

10.9734/mrji/2020/v30i730245 ◽

2020 ◽

pp. 119-125

Author(s):

Gbamelé Kouakou Kan Serges ◽

Yayé Yapi Guillaume ◽

Boyvin Lydie ◽

Bonouman Ira Ama Valerie ◽

Anigbé Amon Maurice ◽

...

Keyword(s):

Antibacterial Properties ◽

Antimicrobial Properties ◽

Antibacterial Activities ◽

Coli Strain ◽

Personal Hygiene ◽

Aureus Strain ◽

Bacterial Strains ◽

E Coli ◽

Skin Conditions ◽

Carapa Procera

Aims: The Carapa procera species is used of traditional for its antimicrobial properties especially for the skin. Its oil is used for various applications including the production of soaps used for personal hygiene and other skin conditions. The purpose of this study is to assess the antibacterial properties of soaps formulated from the oil of this species. Place and Duration of Study: The units of organic biological chemistry and structural organic chemistry of the UFR Structural Sciences of Matter and Technology (Université Félix Houphouet-Boigny) for the manufacture of soaps and the bacteriology unit of the Institut Pasteur de Côte d'Ivoire for bioassays between January 2019 and February 2020. Methodology: The antibacterial evaluation was carried out by the disc method on nutrient agars with determination of bacterial parameters (MIC and MBC). Discs of usuals antibiotics (Tetracycline, Rifampicin and Ampicillin) were used as a control to assess this activity as well as a commercially soap. Results: The inhibition diameters obtained from these different test soaps vary from 16.00 - 17.15 mm; 15.75 - 17.70 mm and 20.33 - 24.00 mm respectively on growth of the bacterial strains E. coli, P. aeruginosa and S. aureus. Unlike unscented soaps, scented soaps have the best inhibition diameter zones values. Moreover, the best MBC values (25 mg/mL) are obtained with the 2 soaps in fresh or aged state on the S. aureus strain against 50 mg/mL on the 2 other strains. In addition, with the exception of the E. coli strain which is resistant to Tetracycline, the 2 other strains are sensitive to all the antibiotics tested. In addition, this study also shows variability in the effect of soaps on these targets. Conclusion: Soaps formulated from Carapa procera oil do indeed possess antibacterial properties that could justify its usage against dermatoses in traditional medicine.

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Effect of Gold Nanoparticles and Silicon on the Bioactivity and Antibacterial Properties of Hydroxyapatite/Chitosan/Tricalcium Phosphate-Based Biomicroconcretes

Materials ◽

10.3390/ma14143854 ◽

2021 ◽

Vol 14 (14) ◽

pp. 3854

Author(s):

Joanna Czechowska ◽

Ewelina Cichoń ◽

Anna Belcarz ◽

Anna Ślósarczyk ◽

Aneta Zima

Keyword(s):

Gold Nanoparticles ◽

Antibacterial Activity ◽

Tricalcium Phosphate ◽

Setting Time ◽

Antibacterial Properties ◽

Bone Substitutes ◽

Bacterial Strains ◽

Setting Times ◽

Biological Studies

Bioactive, chemically bonded bone substitutes with antibacterial properties are highly recommended for medical applications. In this study, biomicroconcretes, composed of silicon modified (Si-αTCP) or non-modified α-tricalcium phosphate (αTCP), as well as hybrid hydroxyapatite/chitosan granules non-modified and modified with gold nanoparticles (AuNPs), were designed. The developed biomicroconcretes were supposed to combine the dual functions of antibacterial activity and bone defect repair. The chemical and phase composition, microstructure, setting times, mechanical strength, and in vitro bioactive potential of the composites were examined. Furthermore, on the basis of the American Association of Textile Chemists and Colorists test (AATCC 100), adapted for chemically bonded materials, the antibacterial activity of the biomicroconcretes against S. epidermidis, E. coli, and S. aureus was evaluated. All biomicroconcretes were surgically handy and revealed good adhesion between the hybrid granules and calcium phosphate-based matrix. Furthermore, they possessed acceptable setting times and mechanical properties. It has been stated that materials containing AuNPs set faster and possess a slightly higher compressive strength (3.4 ± 0.7 MPa). The modification of αTCP with silicon led to a favorable decrease of the final setting time to 10 min. Furthermore, it has been shown that materials modified with AuNPs and silicon possessed an enhanced bioactivity. The antibacterial properties of all of the developed biomicroconcretes against the tested bacterial strains due to the presence of both chitosan and Au were confirmed. The material modified simultaneously with AuNPs and silicon seems to be the most promising candidate for further biological studies.

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Prenylated Flavonoids and C-15 Isoprenoid Analogues with Antibacterial Properties from the Whole Plant of Imperata cylindrica (L.) Raeusch (Gramineae)

Molecules ◽

10.3390/molecules26164717 ◽

2021 ◽

Vol 26 (16) ◽

pp. 4717

Author(s):

Romeo Nago ◽

Paul Nayim ◽

Armelle Mbaveng ◽

James Mpetga ◽

Gabin Bitchagno ◽

...

Keyword(s):

Silica Gel ◽

Methanol Extract ◽

Antibacterial Properties ◽

Imperata Cylindrica ◽

Drug Resistant ◽

Bacterial Strains ◽

Prenylated Flavonoids ◽

Whole Plant ◽

Crude Methanol Extract ◽

Potential Origin

The local botanical Imperata cylindrica in Cameroon was investigated for its antibacterial potency. The methanol extract afforded a total of seven compounds, including five hitherto unreported compounds comprising three flavonoids (1–3) and two C-15 isoprenoid analogues (4 and 5) together with known derivatives (6 and 7). The novelty of the flavonoids was related to the presence of both methyl and prenyl groups. The potential origin of the methyl in the flavonoids is discussed, as well as the chemophenetic significance of our findings. Isolation was performed over repeated silica gel and Sephadex LH-20 column chromatography and the structures were elucidated by (NMR and MS). The crude methanol extract and isolated compounds showed considerable antibacterial potency against a panel of multi-drug resistant (MDR) bacterial strains. The best MIC values were obtained with compound (2) against S. aureus ATCC 25923 (32 µg/mL) and MRSA1 (16 µg/mL).

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Synthesis, Characterization, Antibacterial Properties, and In Vitro Studies of Selenium and Strontium Co-Substituted Hydroxyapatite

International Journal of Molecular Sciences ◽

10.3390/ijms22084246 ◽

2021 ◽

Vol 22 (8) ◽

pp. 4246

Author(s):

Muhammad Maqbool ◽

Qaisar Nawaz ◽

Muhammad Atiq Ur Atiq Ur Rehman ◽

Mark Cresswell ◽

Phil Jackson ◽

...

Keyword(s):

Negative Impact ◽

Antibacterial Properties ◽

Biological Properties ◽

Ion Concentration ◽

Charge Composition ◽

Bacterial Strains ◽

X Ray Diffraction ◽

Molar Ratios ◽

Human Osteosarcoma Cells

In this study, as a measure to enhance the antimicrobial activity of biomaterials, the selenium ions have been substituted into hydroxyapatite (HA) at different concentration levels. To balance the potential cytotoxic effects of selenite ions (SeO32−) in HA, strontium (Sr2+) was co-substituted at the same concentration. Selenium and strontium-substituted hydroxyapatites (Se-Sr-HA) at equal molar ratios of x Se/(Se + P) and x Sr/(Sr + Ca) at (x = 0, 0.01, 0.03, 0.05, 0.1, and 0.2) were synthesized via the wet precipitation route and sintered at 900 °C. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and cell viability were studied. X-ray diffraction verified the phase purity and confirmed the substitution of selenium and strontium ions. Acellular in vitro bioactivity tests revealed that Se-Sr-HA was highly bioactive compared to pure HA. Se-Sr-HA samples showed excellent antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus carnosus) bacterial strains. In vitro cell–material interaction, using human osteosarcoma cells MG-63 studied by WST-8 assay, showed that Se-HA has a cytotoxic effect; however, the co-substitution of strontium in Se-HA offsets the negative impact of selenium and enhanced the biological properties of HA. Hence, the prepared samples are a suitable choice for antibacterial coatings and bone filler applications.

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A New Supported Manganese-Based Coordination Complex as a Nano-Catalyst for the Synthesis of Indazolophthalazinetriones and Investigation of Its Antibacterial Activity

Chemistry ◽

10.3390/chemistry3030056 ◽

2021 ◽

Vol 3 (3) ◽

pp. 783-799

Author(s):

Maryam Ariannezhad ◽

Davood Habibi ◽

Somayyeh Heydari ◽

Vahideh Khorramabadi

Keyword(s):

Antibacterial Activity ◽

Simple Procedure ◽

Condensation Reaction ◽

Antibacterial Properties ◽

Coordination Complex ◽

Bacterial Strains ◽

One Pot ◽

Nano Catalyst ◽

Ft Ir ◽

The One

A new magnetic supported manganese-based coordination complex (Fe3O4@SiO2@CPTMS@MBOL@ Mn) was prepared in consecutive stages and characterized via various techniques (VSM, SEM, TEM, XRD, FT-IR, EDX, TG-DTA, and ICP). To evaluate its application, it was used for synthesis of divers Indazolophthalazinetriones in a simple procedure via the one-pot three-component condensation reaction of aldehydes, dimedone, and phthalhydrazide in ethanol under reflux conditions. The Mn catalyst can be recycled without any noticeable loss in catalytic activity. Additionally, the antibacterial properties of the nano-catalyst were studied against some bacterial strains.

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Synthesis and Characterization of a Bioartificial Polymeric System with Potential Antibacterial Activity: Chitosan-Polyvinyl Alcohol-Ampicillin

Molecules ◽

10.3390/molecules23123109 ◽

2018 ◽

Vol 23 (12) ◽

pp. 3109 ◽

Cited By ~ 3

Author(s):

Andres Bernal-Ballen ◽

Jorge Lopez-Garcia ◽

Martha-Andrea Merchan-Merchan ◽

Marian Lehocky

Keyword(s):

Antibacterial Activity ◽

Polyvinyl Alcohol ◽

Statistical Significance ◽

Antibacterial Properties ◽

Intermolecular Forces ◽

Polymeric System ◽

Bacterial Strains ◽

Natural Polymers ◽

The Matrix ◽

The Individual

Bio-artificial polymeric systems are a new class of polymeric constituents based on blends of synthetic and natural polymers, designed with the purpose of producing new materials that exhibit enhanced properties with respect to the individual components. In this frame, a combination of polyvinyl alcohol (PVA) and chitosan, blended with a widely used antibiotic, sodium ampicillin, has been developed showing a moderate behavior in terms of antibacterial properties. Thus, aqueous solutions of PVA at 1 wt.% were mixed with acid solutions of chitosan at 1 wt.%, followed by adding ampicillin ranging from 0.3 to 1.0 wt.% related to the total amount of the polymers. The prepared bio-artificial polymeric system was characterized by FTIR, SEM, DSC, contact angle measurements, antibacterial activity against Staphylococcus aureus and Escherichia coli and antibiotic release studies. The statistical significance of the antibacterial activity was determined using a multifactorial analysis of variance with ρ < 0.05 (ANOVA). The characterization techniques did not show alterations in the ampicillin structure and the interactions with polymers were limited to intermolecular forces. Therefore, the antibiotic was efficiently released from the matrix and its antibacterial activity was preserved. The system disclosed moderate antibacterial activity against bacterial strains without adding a high antibiotic concentration. The findings of this study suggest that the system may be effective against healthcare-associated infections, a promising view in the design of novel antimicrobial biomaterials potentially suitable for tissue engineering applications.

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Antibacterial Activity of Leukocyte- and Platelet-Rich Plasma: AnIn VitroStudy

BioMed Research International ◽

10.1155/2018/9471723 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

Agata Cieślik-Bielecka ◽

Tadeusz Bold ◽

Grzegorz Ziółkowski ◽

Marcin Pierchała ◽

Aleksandra Królikowska ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Enterococcus Faecalis ◽

Platelet Rich Plasma ◽

Antibacterial Properties ◽

Antimicrobial Effect ◽

Diffusion Method ◽

Bacterial Strains ◽

Bovine Thrombin ◽

Zones Of Inhibition

The aim of the study was to investigate the leukocyte- and platelet-rich plasma (L-PRP) antimicrobial activity. The studied sample comprised 20 healthy males. The L-PRP gel, liquid L-PRP, and thrombin samples were testedin vitrofor their antibacterial properties against selected bacterial strains using the Kirby-Bauer disc diffusion method. Two types of thrombin were used (autologous and bovine). Zones of inhibition produced by L-PRP ranged between 6 and 18 mm in diameter. L-PRP inhibited the growth ofStaphylococcus aureus(MRSA and MSSA strains) and was also active againstEnterococcus faecalisandPseudomonas aeruginosa. There was no activity againstEscherichia coliandKlebsiella pneumoniae. The statistically significant increase of L-PRP antimicrobial effect was noted with the use of major volume of thrombin as an activator. Additionally, in groups where a bovine thrombin mixture was added to L-PRP the zones of inhibition concerning MRSA,Enterococcus faecalis, andPseudomonas aeruginosawere larger than in the groups with autologous thrombin. Based on the conducted studies, it can be determined that L-PRP can evokein vitroantimicrobial effects and might be used to treat selected infections in the clinical field. The major volume of thrombin as an activator increases the strength of the L-PRP antimicrobial effect.

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Pathogenetic Substantiation of the Therapeutic Impact on Microbiota in Irritable Bowel Syndrome

Russian Journal of Gastroenterology Hepatology Coloproctology ◽

10.22416/1382-4376-2019-29-4-7-14 ◽

2019 ◽

Vol 29 (4) ◽

pp. 7-14

Author(s):

Yu. O. Shulpekova ◽

G. H. Babaeva ◽

V. Yu. Rusyaev

Keyword(s):

Irritable Bowel Syndrome ◽

Intestinal Microbiota ◽

Intestinal Bacteria ◽

Host Cells ◽

Intestinal Microbiome ◽

Bacterial Strains ◽

Blastocystis Hominis ◽

Therapeutic Impact ◽

Irritable Bowel ◽

Positive Effect

Aim. This review aims to describe the nature of changes in the intestinal microbiota in irritable bowel syndrome (IBS) and provide a pathogenetic justification of the feasibility of a therapeutic impact on microbiota. General findings. An important aspect of the interaction of intestinal bacteria with the “host” cells is their contact with pattern recognition receptors of enterocytes, dendritic cell receptors, as well as a transcellular transport of antigens in the region of Peyer’s patches. The area of interaction of intestinal bacteria and the human body is not limited to the intestines. Intestinal bacteria demonstrate a significant humoral effect due to signalling molecules, some of which exhibit neurotransmitter properties. The study of the bacterial cross-feeding for various species, i.e. mutual use of nutrient substrates produced by bacteria of various species, is of a great interest. The development of a lowactivity inflammation in IBS can partly be explained by the increased interaction of flagellin with the corresponding receptor, as well as the influx of excess bacteria from the small intestine. The majority of studies on IBS have demonstrated the predominance of intestinal bacteria with pro-inflammatory potential (Enterobacteriaceae) and the lack of bacteria with a pronounced anti-inflammatory, antimicrobial and enzymatic action (Lactobacillus and Bifidobacterium), as well as increased mucus degradation. Similar changes are observed in inflammatory bowel diseases. Reduced microbial diversity increases susceptibility to intestinal infections and parasitoses, including those caused by protozoa conditionally pathogenic for adults, such as Blastocystis hominis hominis, Dientamoeba fragilis, Giardia lamblia. With the help of nutrition correction, the use of probiotics and functional foods containing certain probiotic strains, plant fibres (primarily psyllium) and, in some cases, nonabsorbable antibiotics, a positive effect can be achieved in a significant number of IBS patients. Recent works have shown that clinical improvement is accompanied by a change in the composition of the intestinal microbiota.Conclusion. For the pathogenetic treatment of irritable bowel syndrome, the use of non-drug treatment is justified, such as diet optimization and prescription of plant fibres and probiotic bacterial strains. The positive effect of such an approach is largely determined by modification of the intestinal microbiota composition. This opens up prospects for a further, more targeted impact on the intestinal microbiome.

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Decoding Antibacterial and Antibiofilm Properties of Cinnamon and Cardamom Essential Oils: A Combined Molecular Docking and Experimental Study

10.21203/rs.3.rs-955241/v1 ◽

2021 ◽

Author(s):

Elahe Pourkhosravani ◽

fatemeh dehghan nayeri ◽

Mitra Mohammadi Bazargani

Keyword(s):

Cell Attachment ◽

Antibacterial Properties ◽

Binding Mode ◽

Dilution Method ◽

Antibiofilm Activity ◽

Bacterial Strains ◽

Biofilm Growth ◽

Computational Docking ◽

E Coli ◽

Industrial Strains

Abstract This study sets out to compare the antibacterial and antibiofilm profiles of Ci/Ca EOs alone and in combination together against infectious bacterial strains. MIC assay was carried out to survey the effectiveness of prepared EOs by two-fold serial dilution method and MTT evaluation. Synergic antibacterial properties of EOs against target strains were studied by using checkerboard titration method. Biofilm growth and development were evaluated using CV and XTT reduction assays. Antibacterial activity was observed for EOs against both bacterial strains with stronger activity for CiEO against both bacteria. The synergistic antibacterial effect was observed only against B. subtilis. Based on the FIC index, combinations could not inhibit the growth of E. coli. The pure EOs and their combination inhibited cell attachment for both studied bacteria with stronger effect on E. coli. CV and XTT reduction assays results showed that Ci EO and its combination with CaEO had the highest antibiofilm activity at lowest MIC value 0.08% and 0.04/0.02% against biofilm formed by E. coli and B. subtilis respectively, indicating a high antibiofilm potential. Computational docking analyses also postulated that the active constituents of evaluated EOs have the potential to interact with different bacterial targets, suggested binding mode of action of EOs metabolites. By and large, synergistic anti-biofilm properties of EOs may provide further options for developing novel formula to inhibit a variety of infectious clinical and industrial strains without (or less) toxicity effects on human body.

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