scholarly journals The Essential Oil of Cymbopogon citratus Stapt and Carvacrol: An Approach of the Antitumor Effect on 7,12-Dimethylbenz-[α]-anthracene (DMBA)-Induced Breast Cancer in Female Rats

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3284
Author(s):  
Juan Pedro Rojas-Armas ◽  
Jorge Luis Arroyo-Acevedo ◽  
Miriam Palomino-Pacheco ◽  
Oscar Herrera-Calderón ◽  
José Manuel Ortiz-Sánchez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Essential Oil ◽  
Antitumor Effect ◽  
Tumor Volume ◽  
Physiological Saline ◽  
Saline Group ◽  
Female Rats ◽  
Cymbopogon Citratus ◽  
Breast Tumor Cell ◽  
Group I

C. citratus essential oil and carvacrol have shown an antitumor effect on breast tumor cell lines; the main objective of this research was to evaluate the antitumor effect of the essential oil of Cymbopogon citratus (EOCc) and carvacrol on 7,12-dimethylbenz [a] anthracene (DMBA)-induced breast cancer in female rats. Cancer was induced by a single administration of DMBA at dose of 80 mg/kg body weight (BW). A total of 54 female Holtzman rats were randomly assigned into 9 groups (n = 6). Group I: PS (Physiological saline); Group II: DMBA; Groups III, IV, and V: DMBA + EOCc at doses of 50, 100 and 200 mg/kg/day BW, respectively; Groups VI, VII, and VIII: DMBA + carvacrol at doses of 50, 100 and 200 mg/kg/day BW, respectively; and group IX: DMBA + EOCc + carvacrol at doses of 100 mg/kg/day BW. The treatment lasted 14 weeks. As results, EOCc showed a reduction in tumors as well as necrosis and mitosis. Animals treated with carvacrol did not show necrosis, mitosis, or infiltration. Carvacrol at dose of 100 mg/kg/day BW revealed a significant decrease in the cumulative tumor volume down to 0.11 ± 0.05 cm3 compared to 0.38 ± 0.04 cm3 of the DMBA group (p < 0.01). It is concluded that EOCc and carvacrol had an antitumor effect on DMBA-induced breast cancer in female rats.

scholarly journals Triazine Derivative as Putative Candidate for the Reduction of Hormone-Positive Breast Tumor: In Silico, Pharmacological, and Toxicological Approach

2021 ◽  
Vol 12 ◽  
Author(s):  
Muhammad Tayyab Imtiaz ◽  
Fareeha Anwar ◽  
Uzma Saleem ◽  
Bashir Ahmad ◽  
Sundas Hira ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Tumor ◽  
In Silico ◽  
Enzyme Level ◽  
Saline Group ◽  
Female Rats ◽  
Beneficial Effects ◽  
Group I

Background and objectives: Breast cancer is a heterogeneous disease that poses the highest incidence of morbidity among women and presents many treatment challenges. In search of novel breast cancer therapies, several triazine derivatives have been developed for their potential chemotherapeutic activity. This study aims to evaluate the N-nitroso-N-methyl urea (NMU)–induced anti–mammary gland tumor activity of 2,4,6 (O-nitrophenyl amino) 1,3,5-triazine (O-NPAT).Methods: The in silico modeling and in vitro cytotoxicity assay were performed to strengthen the research hypothesis. For in vivo experimentation, 30 female rats were divided into five groups. Group I (normal control) received normal saline. Group II (disease control) received NMU (50 mg/kg). Group III (standard control) was treated with tamoxifen (5 mg/kg). Groups IV and V received O-NPAT at a dose level of 30 and 60 mg/kg, respectively. For tumor induction, 3 intraperitoneal doses of NMU were given at a 3-week interval, whereas all treatment compounds were administered orally for 14 consecutive days. Biochemical and oxidative stress markers were estimated for all experimental animals. DNA strand breakage alongside inflammatory markers was also measured for the analysis of inflammation. The hormonal profile of progesterone and estrogen was also estimated.Results: The test compound presented a significant reduction in organ weight and restored the hepatic and renal enzymes. O-NPAT treatments enhanced the antioxidant enzyme level of catalase (CAT), superoxide dismutase (SOD), and total sulfhydryl (TSH), with a highly significant reduction in lactate dehydrogenase (LDH) and lipid peroxidation. Also, the decrease in fragmented DNA, hormonal levels (estradiol and progesterone), and inflammatory cytokines (IL-6 and TNF-α) justified the dosage efficacy further supported by histopathological findings.Conclusion: All results indicated the anti–breast tumor activity of O-NPAT and presented its possibility of exploitation for beneficial effects in breast cancer treatment.

Antitumor Effect of Mesenchymal Stem Cells from Murine in Breast Cancer in Female Rats

2018 ◽  
Vol 35 (1) ◽  
pp. 369-374
Author(s):  
Omayma A.R. AbouZaid ◽  
Laila A Rashed ◽  
S. M. El-Sonbaty ◽  
Aboel-Ftouh A. I
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Antitumor Effect ◽  
Female Rats

scholarly journals Effects of Enoxaparin Emulsion on Dimethylbenzanthracene-induced Breast Cancer in Female Rats

2018 ◽  
Vol 5 (4) ◽  
pp. 23
Author(s):  
Bolandpayeh M ◽  
Hassanpour-Ezzati M ◽  
Mousavi Z
Keyword(s):  
Breast Cancer ◽  
Tumor Growth ◽  
Tumor Volume ◽  
Positive Control ◽  
Female Rats ◽  
Positive Control Group ◽  
Control Group ◽  
Dependent Manner ◽  
Angiogenic Proteins ◽  
Dose Dependent

Introduction: Enoxaparin is an anticoagulant medication. Anticoagulation inhibits tumor cell-mediated release of angiogenic proteins and diminishes angiogenic response. Angiogenesis is an important event in various cancers such as breast cancer. Angiogenesis provide oxygen and nutrients to tumor cells and causes tumor progression. The aim of the present study was to evaluate the anti-angiogenesis effect of an enoxaparin cream on breast cancer induced by dimethylbenzanthracene in rats. Methods: In this experimental in vivo study, 50 Wistar female rats were divided into negative control (vehicle), positive control (cream base), and 3 groups with enoxaparin treatment (40, 60, and 80 mg/ml). After one month of treatment along with breast cancer induction by dimethylbenzanthracene, breast tissue samples were isolated and stained with hematoxylin-eosin, and tumor growth suppression rate was calculated. Tumor size (length and width) was measured using a clipper, and the tumor volume was calculated using the following formula: V = (L × W × W)/2, where V is tumor volume, W is tumor width, L is tumor length. The data were analyzed using one-way ANOVA and Tukey’s post hoc test. Results: Tumor suppression was significantly increased in enoxaparin treatment groups compared to the positive control group (40 mg/ml of enoxaparin treated versus positive control group; P = 0.017, 60 mg/ml of enoxaparin treated versus positive control; P = 0.015, 40 mg/ml of enoxaparin treated versus positive control; P = 0.009, 60 mg/ml of enoxaparin treated versus 40 mg/ml of enoxaparin treated; P = 0.019, and 80 mg/ml of enoxaparin treated versus 40 mg/ml of enoxaparin treated; P = 0.011 in a dose-dependent manner. Conclusion: Enoxaparin inhibits breast cancer in a dose-dependent manner. The application of enoxaparin cream in patients with breast cancer may considerably reduce tumor growth. 

Modulatory effects of l-carnitine on tamoxifen toxicity and oncolytic activity

2013 ◽  
Vol 33 (9) ◽  
pp. 968-979 ◽  
Author(s):  
AB Ibrahim ◽  
HH Mansour ◽  
SA Shouman ◽  
AA Eissa ◽  
SM Abu El Nour
Keyword(s):  
Antitumor Effect ◽  
Liver Enzymes ◽  
Combined Treatment ◽  
Lipid Peroxides ◽  
Ehrlich Ascites Carcinoma ◽  
Treated Group ◽  
Female Rats ◽  
Sod Activity ◽  
Beneficial Effects ◽  
Group I

The aim of this study was to investigate the protective effect of l-carnitine (l-CAR) in tamoxifen (TAM)-induced toxicity and antitumor activity. Adult female rats were randomly divided into four groups. Group I was served as control, groups II and III were treated with TAM (10 mg/kg, periorally) and l-CAR (300 mg/kg, intraperitoneally), respectively, while group IV was treated with both compounds. The treatment continued daily for 28 days. Administration of TAM resulted in significant increase in serum lipid profiles, liver enzymes, and bilirubin level. TAM produced a significant increase in lipid peroxides (LPO) level and nonsignificant change in nitrogen oxide (NO( x)) level accompanied with significant decrease in superoxide dismutase (SOD) activity of hepatic and uterus tissues and significant decrease in glutathione (GSH) content of uterus tissue. Administration of l-CAR for 1 h prior to TAM treatment decreased serum lipids and liver enzymes significantly and significantly increased SOD activity in liver and uterus tissues compared with TAM-treated group. Furthermore, it restored LPO and GSH levels and increased NO( x) level in uterus tissue. DNA fragmentation and the apoptotic marker, caspase-3, were not detected in the liver of all treated groups. Histopathologically, alterations in the liver and uterus structures after TAM treatment, which was attenuated after l-CAR administration. The antitumor effect and survival of the combined treatment of Ehrlich ascites carcinoma (EAC)-bearing mice was less than each one alone. l-CAR interestingly increased survival rate of EAC-bearing mice more than TAM-treated group. In conclusion, l-CAR has beneficial effects regarding TAM toxicity; however, it interferes with its antitumor effect.

scholarly journals Preserved Ovarian Function Following Toxicity With Doxorubicin in Rats: Protective Effect of Nasturtium Officinale Extract

2021 ◽  
Vol 15 (1) ◽  
pp. 57-64
Author(s):  
Parastou Rad ◽  
◽  
Fahimeh Safari ◽  
Jamshid Mohammadi ◽  
Hamdollah Delaviz ◽  
...  
Keyword(s):  
Ovarian Function ◽  
Serum Levels ◽  
Saline Group ◽  
Female Rats ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Nasturtium Officinale ◽  
Group I ◽  
The Mean

Background: Chemotherapy agents can cause ovarian dysfunction and eventually lead to infertility. This study investigated the effect of nasturtium officinale extract on the ovarian function following the toxicity induced by doxorubicin in female rats. Methods: Forty eight female Wistar rats (180-210g) were randomly divided in six groups as follows: Group I, normal rats receiving 1ml normal saline; Group II and III receiving 25 and 75 mg/kg of the extract daily by gavage for 21 days. Groups IV, V and VI receiving 10 mg/kg doxorubicin intraperitoneally on the first day. In addition, Group IV and V received 25 and 75 mg/kg of the extract, respectively. The serum levels of estrogen, progesterone, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and ovarian Malondialdehyde (MDA) were determined after 21 days of treatment. The mean numbers of various graafian follicles and corpus lutea were recorded after treatment. Results: The mean serum FSH level in Group VI (0.11±0.01) significantly reduced compared to those in Groups II (0.21±0.05) and III (0.23±0.01), (P<0.05). The mean serum LH and estrogen levels in Group VI (0.16±0.08) reduced insignificantly compared to those in the controls (0.21±0.02), and in Groups II (0.23±0.03) and III (0.22±0.09). A significant reduction in the number of primary, secondary and graafian follicles were observed in Group VI compared to the control group (P<0.05). The serum MDA level significantly declined in Group V compared to that in Group VI (P<0.05). Conclusion: The nasturtium officinale extract attenuated the toxic effect of doxorubicin on the rat ovaries and protected the cell division in the follicles and the oocytes maturation.

scholarly journals Histopathological evaluation of Senecio rhizomatus Rusby in 7,12-dimethylbenz(α) anthracene-induced breast cancer in female rats

2021 ◽  
Vol 14 (3) ◽  
pp. 569-577
Author(s):  
Jorge Luis Arroyo-Acevedo ◽  
Oscar Herrera-Calderon ◽  
Juan Pedro Rojas-Armas ◽  
Roberto Chávez-Asmat ◽  
James Calva ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antioxidant Activity ◽  
Pharmacological Study ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Female Rats ◽  
Chemical Components ◽  
Experimental Conditions ◽  
Group I ◽  
Physiological Serum

Background and Aim: Senecio rhizomatus Rusby (SrR) is a medicinal plant of the Asteraceae family and traditionally consumed as infusion in the Andean region from Peru for inflammatory disorders. This study aimed to determine the histopathological changes afforded by SrR in 7, 12-dimethylbenz[α]anthracene (DMBA)-induced breast cancer (BC) in rats. Materials and Methods: An ethanolic extract of SrR aerial parts was prepared by maceration with 96% ethanol, and the chemical components were identified by gas chromatography coupled to mass spectrometry; the antioxidant activity was determined by 1,1-diphenyl-2-picril-hidrazil (DPPH) assay; and the acute toxicity was assessed according to the OCED 423 guidelines. In a pharmacological study, 30 female Holztman rats were distributed randomly into five groups, as follows. Group I: Negative control (physiological serum, 2 mL/kg); Group II. DMBA (80 mg/Kg body weight); and Groups III, IV, and V: DMBA + ethanol extract of SrR at doses of 10, 100, and 200 mg/kg, respectively. Results: The antioxidant activity of the SrR extract against DPPH was 92.50% at 200 μg/mL. The oral administration of SrR at doses of 50, 300, 2000, and 5000 mg/kg did not show any clinical evidence of toxicity or occurrence of death. The groups that received SrR presented a lower frequency of tumors and a cumulative tumor volume compared with the DMBA group (p<0.05); the DMBA group exhibited a higher incidence of necrosis and moderate mitosis, up to 66.67% and 100.00%, respectively. Finally, infiltrating carcinoma with extensive tumor necrosis was evidenced. Conclusion: In experimental conditions, the ethanolic extract of SrR had a protective effect in DMBA-induced BC in female rats. Furthermore, the antioxidant activity of its main phytochemicals could be responsible for the effect observed, and SrR seems to be a safe extract in the preclinical phase.

scholarly journals Lemongrass (Cymbopogon Citratus) Essential Oil Inhibits Candida Albicans Growth in Vitro

Biomedika ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 142-147
Author(s):  
Yoga Mulia Pratama ◽  
Bryan Pandu Permana
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
High Morbidity ◽  
Cymbopogon Citratus ◽  
Group I ◽  
Inhibition Zones

Candidiasis is an important nosocomial infection with high morbidity value, high mortality rate, and expensive clinical cost. However, public access to well-developed treatment is not acquired. Current medicines mostly used like -azol drugs had been showing the resistant effects because of the long period use of the same medicines. Alternative medicines like herb medicine are discussed to reduce multi-resist infections, such as lemongrass Cymbopogon citratus essential oil. This study aims to know the effect of lemongrass essential oil on the growth of Candida albicans in vitro. Laboratory experimental (in vitro) was conducted in this research. Candida albicans strains were being used as the objects, which were picked by random sampling. Candida albicans were divided into 12 groups of treatment, the group I was treated with ethanol 96% as the negative control, group II with fluconazole 25μg as the positive control, and group III-XII with Cymbopogon citratus essential oil with 10%, 20% to 100% concentrated. The diameter of inhibition zones was measured after 2x24 hours incubation. The data was analyzed by post-hoc Mann Whitney test with SPSS 18.0 (p<0.05 considered as significant). Cymbopogon citratus essential oil showed antifungal activity to the Candida albicans began in the 10% to 100% concentration (p<0.05). Inhibition zones with the 50% to 100% concentration had similar results to the positive control (p>0.05). The Cymbopogon citratus essential oil has an antifungi effect toward Candida albicans in vitro significantly by the negative control.

Antitumor effect and potentiation of cytotoxic drug activity of a dual topoisomerase inhibitor on breast cancer

2019 ◽  
Author(s):  
I Flörkemeier ◽  
TN Steinhauer ◽  
MT van Mackelenbergh ◽  
B Clement ◽  
DO Bauerschlag
Keyword(s):  
Breast Cancer ◽  
Antitumor Effect ◽  
Cytotoxic Drug ◽  
Drug Activity ◽  
Topoisomerase Inhibitor

ANTITUMOR EFFECT OF SUNITINIB AND BORTEZOMIB ON BREAST CANCER CELL LINE IN VITRO

2017 ◽  
Vol 63 (1) ◽  
pp. 141-145
Author(s):  
Yuliya Khochenkova ◽  
Eliso Solomko ◽  
Oksana Ryabaya ◽  
Yevgeniya Stepanova ◽  
Dmitriy Khochenkov
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Antitumor Effect ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Actual Problem

The discovery for effective combinations of anticancer drugs for treatment for breast cancer is the actual problem in the experimental chemotherapy. In this paper we conducted a study of antitumor effect of the combination of sunitinib and bortezomib against MDA-MB-231 and SKBR-3 breast cancer cell lines in vitro. We found that bortezomib in non-toxic concentrations can potentiate the antitumor activity of sunitinib. MDA-MB-231 cell line has showed great sensitivity to the combination of bortezomib and sunitinib in vitro. Bortezomib and sunitinib caused reduced expression of receptor tyrosine kinases VEGFR1, VEGFR2, PDGFRa, PDGFRß and c-Kit on HER2- and HER2+ breast cancer cell lines

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