scholarly journals Green Synthesis of Silver Nanoparticles with Antibacterial Activity Using Various Medicinal Plant Extracts: Morphology and Antibacterial Efficacy

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1005
Author(s):  
Aneta Salayová ◽  
Zdenka Bedlovičová ◽  
Nina Daneu ◽  
Matej Baláž ◽  
Zdenka Lukáčová Bujňáková ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Plant Extracts ◽  
Correlation Spectroscopy ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Selected Area Electron Diffraction ◽  
Medicinal Plant Extracts ◽  
Vis Spectroscopy ◽  
Size And Morphology

A green synthetic route for the production of silver nanoparticles (AgNPs) using five different aqueous plant extracts, namely, Berberis vulgaris, Brassica nigra, Capsella bursa-pastoris, Lavandula angustifolia and Origanum vulgare, was investigated in this study. The present work demonstrates the influence of plant extract composition (antioxidant and total phenolic content) on the size and morphology of the produced AgNPs. The biosynthetic procedure was rapid and simple and was easily monitored via colour changes and ultraviolet and visible (UV-Vis) spectroscopy. Subsequently, measurement of zeta potential (ZP), photon cross-correlation spectroscopy (PCCS), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and selected area electron diffraction (SAED) analysis were employed to characterise the as-synthesised nanoparticles. The XRD investigation confirmed the presence of Ag0 in the nanoparticles, and interactions between the bioactive compounds of the plants and the produced AgNPs were evident in the FTIR spectra. TEM indicated that the nanoparticles exhibited a bimodal size distribution, with the smaller particles being spherical and the larger having a truncated octahedron shape. In addition, the antimicrobial activity of the AgNPs was tested against five bacterial strains. All synthesised nanoparticles exhibited enhanced antimicrobial activity at a precursor concentration of 5 mM compared to the control substance, gentamicin sulphate, with the best results observed for AgNPs prepared with B. nigra and L. angustifolia extracts.

scholarly journals Characterization and Antibacterial Response of Silver Nanoparticles Biosynthesized Using an Ethanolic Extract of Coccinia indica Leaves

Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 97
Author(s):  
Suresh V. Chinni ◽  
Subash C. B. Gopinath ◽  
Periasamy Anbu ◽  
Neeraj Kumar Fuloria ◽  
Shivkanya Fuloria ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Ethanolic Extract ◽  
Spherical Shape ◽  
Bacterial Strains ◽  
X Ray ◽  
Coccinia Indica ◽  
Antibacterial Potential

The present study was planned to characterize and analyze the antimicrobial activity of silver nanoparticles (AgNP) biosynthesized using a Coccinia indica leaf (CIL) ethanolic extract. The present study included the preparation of CIL ethanolic extract using the maceration process, which was further used for AgNP biosynthesis by silver nitrate reduction. Biosynthetic AgNPs were characterized using UV–Visible spectrometry, zeta potential analysis, transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and energy-dispersive X-ray (EDX) spectrometry. The biogenic AgNP and CIL extracts were further investigated against different bacterial strains for their antimicrobial activity. The surface plasmon resonance (SPR) signal at 425 nm confirmed AgNP formation. The SEM and TEM data revealed the spherical shape of biogenic AgNPs and size in the range of 8 to 48 nm. The EDX results verified the presence of Ag. The AgNPs displayed a zeta potential of −55.46 mV, suggesting mild AgNP stability. Compared to Gram-positive bacteria, the biogenic AgNPs demonstrated high antibacterial potential against Gram-negative bacteria. Based on the results, the current study concluded that AgNPs based on CIL extract have strong antibacterial potential, and it established that AgNP biosynthesis using CIL ethanol extract is an effective process.

scholarly journals Green Synthesis and Characterization of Pullulan Mediated Silver Nanoparticles through Ultraviolet Irradiation

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2382 ◽  
Author(s):  
Muhammad Jamshed Khan ◽  
Suriya Kumari ◽  
Kamyar Shameli ◽  
Jinap Selamat ◽  
Awis Qurni Sazili
Keyword(s):  
Silver Nanoparticles ◽  
Uv Irradiation ◽  
Irradiation Time ◽  
Edible Films ◽  
Crystallographic Structure ◽  
Face Centered Cubic ◽  
Selected Area Electron Diffraction ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Average Lattice

Nanoparticles (NPs) are, frequently, being utilized in multi-dimensional enterprises. Silver nanoparticles (AgNPs) have attracted researchers in the last decade due to their exceptional efficacy at very low volume and stability at higher temperatures. Due to certain limitations of the chemical method of synthesis, AgNPs can be obtained by physical methods including sun rays, microwaves and ultraviolet (UV) radiation. In the current study, the synthesis of pullulan mediated silver nanoparticles (P-AgNPs) was achieved through ultraviolet (UV) irradiation, with a wavelength of 365 nm, for 96 h. P-AgNPs were formed after 24 h of UV-irradiation time and expressed spectra maxima as 415 nm, after 96 h, in UV-vis spectroscopy. The crystallographic structure was “face centered cubic (fcc)” as confirmed by powder X-ray diffraction (PXRD). Furthermore, high resolution transmission electron microscopy (HRTEM) proved that P-AgNPs were covered with a thin layer of pullulan, with a mean crystalline size of 6.02 ± 2.37. The average lattice fringe spacing of nanoparticles was confirmed as 0.235 nm with quasi-spherical characteristics, by selected area electron diffraction (SAED) analysis. These green synthesized P-AgNPs can be utilized efficiently, as an active food and meat preservative, when incorporated into the edible films.

Studies on Phytochemical, Antimicrobial activity and Micro propagation of Medicinal plants from Eastern Ghats of Andhra Pradesh

2021 ◽  
pp. 111-115
Author(s):  
Darsi Phebe Sarah Koti Ratnam
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Andhra Pradesh ◽  
Eastern Ghats ◽  
Total Phenolic ◽  
Rauwolfia Serpentina ◽  
Alstonia Scholaris ◽  
Soxhlet Apparatus ◽  
Medicinal Plant Extracts ◽  
Micro Propagation

The aim of the present study was to investigate the presence of phyto chemical, antimicrobial activities and micro propagation of the selected medicinal plants i.e. Rauwolfia serpentina, Adhatoda vasica and Alstonia scholaris. These endemic plants belong to Apocynaceae and were collected from higher altitudes of Eastern Ghats, Lambasingi forest region, Andhra Pradesh India. To determine the total phenolic and flavonoid contents, Soxhlet apparatus was used for this study. Solvents used were in this study are water, ethyl acetate, chloroform and methanol. Among them the solvent methanolic extract of Rauwolfia serpentina (57.15±1.2) and Alstonia scholaris (55.06±0.7) showed high content of saponins. The test microrganism which were studied against the efficacy of selected medicinal plant extracts were, two bacterial strians i.e., Streptococcus pyogenes, Pseudomonas aeruginosa and two fungal strains Aspergillus niger and Vibrio cholera. The antimicrobial activity was proved that the methanol extracts was found to be maximum antimicrobial growth inhibition. The simple and effective protocol was developed to propagate the Rauwolfia plant from nodal explants. Maximum no of 95% plantlets regenerated successfully. These propagated plantlets were hardened to survive in vivo conditions.

PHYTO-MEDIATED SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF „BUGLOSSOIDES PURPUROCAERULEA“ (BORAGINACEAE) AND THEIR BIOACTIVITY

2021 ◽  
Author(s):  
Jelena S. Katanić Stanković ◽  
◽  
Nikola Srećković ◽  
Vladimir Mihailović
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Bacterial Strains ◽  
Penicillium Species ◽  
Microdilution Method ◽  
Biological Potential ◽  
Vis Spectroscopy

In this study, silver nanoparticles (AgNPs) have been synthesized using the aqueous extract of the aerial parts of B. purpurocaerulea, collected in Serbia. B. purpurocaerulea silver nanoparticles (Bp– AgNPs) synthesis was confirmed using UV-Vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The biological potential of synthesized Bp-AgNPs was evaluated in vitro using ABTS assay for determining free radical scavenging potential and microdilution method for analysis of antimicrobial properties. Bp-AgNPs showed high antioxidant activity similar to Bp-extract, comparable to BHT. The synthesized nanoparticles exerted remarkable antibacterial effects, with minimal inhibitory concentration (MIC) values below 20 µg/mL. In the case of some bacterial strains, the results of Bp– AgNPs were comparable or similar to standard antibiotic erythromycin. The antifungal activity of Bp– AgNPs was moderate for most of the used strains. Nevertheless, several fungi were resistant to the NPs action, while two tested Penicillium species were extremely sensitive on Bp-AgNPs with MIC lower than 40 µg/mL. The antimicrobial properties of Bp-AgNPs can be useful for the development of new NPs-containing products.

scholarly journals Anticancer and Antimicrobial Activity Evaluation of Cowpea-Porous-Starch-Formulated Silver Nanoparticles

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Shiara Ramdath ◽  
John Mellem ◽  
Londiwe Simphiwe Mbatha
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Cell Viability ◽  
High Dose ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Anticancer Drug Delivery ◽  
Ic50 Values ◽  
Biosynthesized Agnps

Health issues involving inadequate treatment of diseases such as cancer and microbial infections continue to be the subject of much ongoing recent research. Biosynthesized silver nanoparticles (AgNPs) were characterized using Transmission Electron Microscopy (TEM), Zeta Sizer, Ultraviolet (UV), and Fourier Transform Infrared (FTIR) spectroscopy. Their antimicrobial activity was evaluated on selected Gram-positive and Gram-negative bacterial strains, using the disc diffusion and broth dilution assays. Cell viability profiles were evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and apoptosis studies on selected human noncancer and cancer cells. The biosynthesized AgNPs were evaluated to be spherical clusters, with sizes between 40 and 70 nm. The absorption peak at 423 nm and the presence of polyphenols confirmed the synthesis and stabilization of these tested AgNPs. The AgNPs showed a good stability of −23.9 ± 1.02 mV. Good antimicrobial activity (6.0–18.0 mm) was seen on all tested bacteria at a minimum inhibitory concentration (MIC) ranging from 5 to 16 μg/ml, with the highest activity seen against Gram-negative Escherichia coli (18 ± 0.5 mm), and the lowest activity was seen against Gram-positive Listeria monocytogenes (6.0 ± 0.4 mm) after treatment with the AgNPs. These NPs showed a concentration-dependent and cell-specific cytotoxicity with low IC50 values (41.7, 56.3, and 63.8 μg/ml). The NPs were well tolerated by tested cells as indicated by a more than 50% cell viability at the high dose tested and low apoptotic indices (<0.2). These findings indicated that these biosynthesized AgNPs showed great potential as effective antibacterial agents and anticancer drug delivery modalities.

scholarly journals Biophysical and Characterizations of Silver Nanoparticles used as Salmonella typhi Detector

2014 ◽  
Vol 2 (4) ◽  
pp. 510-515
Author(s):  
Hala Moustafa Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Combined Action ◽  
Hog Plum ◽  
Transmission Electronmicroscopy

The present study mainly focuses of combined action of Nepali hog plum as well as citrate synthesized silver nanoparticles (AgNPs) and Amikacin, as an antibiotic. The synergistic actions of citrate stabilized silver nanoparticles (AgNPs with chem) were compared with that of Nepali hog plum Choerospondia saxillaris (Lapsi) synthesized silver nanoparticles (AgNPs with plant), together with action of antibiotic onselected bacterial strains of Salmonella typhi. The synthesized AgNPs were characterized through UV-Vis spectroscopy, Transmission electronmicroscopy and X-ray diffraction technique. The size of the synthesized silver nanoparticles was measured by Transmission Electron Microscope (TEM) and X-ray diffraction (XRD).DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11127 Int J Appl Sci Biotechnol, Vol. 2(4): 510-515 

scholarly journals Antioxidant and cytotoxic studies of Acacia nilotica twig extract and their green synthesized silver nanoparticles

2020 ◽  
Vol 9 (2) ◽  
pp. 975-980 ◽  
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Cell Lines ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Acacia Nilotica ◽  
Total Phenolic ◽  
Hek 293 ◽  
Selected Area Electron Diffraction ◽  
Wide Range

Acacia nilotica (L.) Delile is well known as “Desi Kikar”or Babul in India that possesses a wide range of pharmacological activities. In the present study, Acacia nilotica twig extract and its synthesized silver nanoparticles (AgNPs) were evaluated for total phenolic content (TPC), antioxidant activity and cytotoxic effects. Characterization of AgNPs was done by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Selected area electron diffraction (SAED) techniques. Antioxidant potential was determined using different assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power and β-carotene linoleic acid. Cytotoxicity was tested by 3-(4,5-dimethyl-2-yl)-2,5-diphynyl tetrazolium bromide (MTT) assay on Human embryonic kidney (HEK)-293 cell lines. The results indicated that AgNPs exhibited higher antioxidant activity (81.11 %) and TPC (57.35 mg of GAE/mL of extract) as compare to plant extract. A positive correlation was observed between the TPC and antioxidant activities. The inhibitory concentration (IC50) of A. nilotica extract and AgNPs was 52.08µg/mL and 56.82µg/mL respectively. Cytotoxicity against HEK-293 cell lines was dose dependent. Accordingly, it is summarized that A. nilotica based AgNPs could serve as a potential antioxidant for therapeutic purposes.

Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection

2018 ◽  
Vol 78 (1) ◽  
pp. 235-246 ◽  
Author(s):  
D. Dixit ◽  
D. Gangadharan ◽  
K. M. Popat ◽  
C. R. K. Reddy ◽  
M. Trivedi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Inductively Coupled Plasma ◽  
Antibacterial Agents ◽  
Bacterial Count ◽  
Water Disinfection ◽  
Bacterial Strains ◽  
Vis Spectroscopy ◽  
Green Seaweed

Abstract A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) employing the aqueous extract derived from natural and renewable source namely tropical benthic green seaweed Ulva flexuosa was developed. This route involves the reduction of Ag+ ions anchored onto macro porous methacrylic acid copolymer beads to AgNPs for employing them as antibacterial agents for in vitro water disinfection. The seaweed extract itself acts as a reducing and stabilizing agent and requires no additional surfactant or capping agent for forming the AgNPs. The nanoparticles were analyzed using high-resolution transmission electron microscopy, UV–Vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis and inductively coupled plasma optical emission spectroscopy. The study elucidates that such biologically synthesized AgNPs exhibit potential antibacterial activity against two Gram positive (Bacillus subtilis, Staphylococcus aureus) and two Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacterial strains tested. The bacterial count in treated water was reduced to zero for all the strains. Atomic force microscopy was performed to confirm the pre- and post-state of the bacteria with reference to their treatment with AgNPs. Attributes like facile environment-friendly procedure, stability and high antibacterial potency propel the consideration of these AgNPs as promising antibacterial entities.

Ampicillin-augmented silver nanoparticles for synergistic antimicrobial response: A promising therapeutic approach

2021 ◽  
Vol 22 ◽  
Author(s):  
Kashan Khan ◽  
Mohd Aamir Qureshi ◽  
Ameer Azam ◽  
Moinuddin ◽  
Javed Musarrat ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Bacterial Infections ◽  
Colloidal Stability ◽  
Resistant Bacteria ◽  
Antimicrobial Drugs ◽  
Vis Spectroscopy ◽  
Microbial Infections ◽  
Ft Ir

Aims: Globally Scientists are working to find more efficient antimicrobial drugs to treat microbial infections and kill drug-resistant bacteria. Background: Despite the availability of numerous antimicrobial drugs bacterial infections still poses a serious threat to global health. Due to a constant decline in the effectiveness of antibiotics owing to their repeated exposure as well as shortlasting antimicrobial activity, led to the demand for developing novel therapeutic agents capable of controlling microbial infections. Objective: In this study, we report antimicrobial activity of chemically synthesized silver nanoparticles (cAgNPs) augmented with ampicillin (amp) in order to increase antimicrobial response against Escherichia coli (gram –ve), Staphylococcus aureus (gram +ve) and Streptococcus mutans (gram +ve). Methods: Nanostructure, colloidal stability, morphology and size of cAgNPs before and after functionalization were explored by UV-vis spectroscopy, FT-IR, zeta potential and TEM. The formation and functionalization of cAgNPs was confirmed from UV-vis spectroscopy and FT-IR patterns. From TEM the average sizes of cAgNPs and cAgNP-amp were found to be 13 and 7.8 nm respectively, and change in colloidal stability after augmentation was confirmed from zeta potential values. The antimicrobial efficacies of cAgNP-amp and cAgNPs against E. coli S. aureus and S. mutans were studied by determining minimum inhibitory concentrations (MICs), zone of inhibition, assessment of viable and non-viable bacterial cells and quantitative assessment of biofilm. Results & Discussion: Our results revealed cAgNP-amp to be highly bactericidal compared to cAgNPs or amp alone. The nano-toxicity studies indicated cAgNP-amp to be less toxic compared to cAgNPs alone. Results: This study manifested that cAgNPs show synergistic antimicrobial effect when they get functionalized with amp suggesting their application in curing long-term bacterial infections.

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