scholarly journals The Therapeutic Potential of Breast Milk-Derived Extracellular Vesicles

Nutrients ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 745 ◽  
Author(s):  
Jeffrey D. Galley ◽  
Gail E. Besner
Keyword(s):  
Breast Milk ◽  
Extracellular Vesicles ◽  
Therapeutic Potential ◽  
Gastrointestinal Disease ◽  
The Body ◽  
Parent Cell ◽  
Therapeutic Effects ◽  
Human Breast Milk ◽  
Therapeutic Benefits ◽  
Health And Disease

In the past few decades, interest in the therapeutic benefits of exosomes and extracellular vesicles (EVs) has grown exponentially. Exosomes/EVs are small particles which are produced and exocytosed by cells throughout the body. They are loaded with active regulatory and stimulatory molecules from the parent cell including miRNAs and enzymes, making them prime targets in therapeutics and diagnostics. Breast milk, known for years to have beneficial health effects, contains a population of EVs which may mediate its therapeutic effects. This review offers an update on the therapeutic potential of exosomes/EVs in disease, with a focus on EVs present in human breast milk and their remedial effect in the gastrointestinal disease necrotizing enterocolitis. Additionally, the relationship between EV miRNAs, health, and disease will be examined, along with the potential for EVs and their miRNAs to be engineered for targeted treatments.

Effects of Extracellular Vesicles Derived from Mesenchymal Stem/Stromal Cells on Liver Diseases

2019 ◽  
Vol 14 (5) ◽  
pp. 442-452 ◽  
Author(s):  
Wenjie Zheng ◽  
Yumin Yang ◽  
Russel Clive Sequeira ◽  
Colin E. Bishop ◽  
Anthony Atala ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Extracellular Vesicles ◽  
Stromal Cells ◽  
Liver Diseases ◽  
Therapeutic Potential ◽  
Mechanisms Of Action ◽  
Therapeutic Effects ◽  
Chronic Liver Injury ◽  
Mediated Effects ◽  
Therapeutic Benefits

Therapeutic effects of Mesenchymal Stem/Stromal Cells (MSCs) transplantation have been observed in various disease models. However, it is thought that MSCs-mediated effects largely depend on the paracrine manner of secreting cytokines, growth factors, and Extracellular Vesicles (EVs). Similarly, MSCs-derived EVs also showed therapeutic benefits in various liver diseases through alleviating fibrosis, improving regeneration of hepatocytes, and regulating immune activity. This review provides an overview of the MSCs, their EVs, and their therapeutic potential in treating various liver diseases including liver fibrosis, acute and chronic liver injury, and Hepatocellular Carcinoma (HCC). More specifically, the mechanisms by which MSC-EVs induce therapeutic benefits in liver diseases will be covered. In addition, comparisons between MSCs and their EVs were also evaluated as regenerative medicine against liver diseases. While the mechanisms of action and clinical efficacy must continue to be evaluated and verified, MSCs-derived EVs currently show tremendous potential and promise as a regenerative medicine treatment for liver disease in the future.

scholarly journals Extracellular Vesicles of Mesenchymal Stem Cells: Therapeutic Properties Discovered with Extraordinary SuccessOK

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 667
Author(s):  
Gabriella Racchetti ◽  
Jacopo Meldolesi
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Immune Responses ◽  
Extracellular Vesicles ◽  
Immune Regulation ◽  
Great Increase ◽  
The Body ◽  
Cell Aging ◽  
Therapeutic Effects ◽  
Therapeutic Properties

Mesenchymal stem cells (MSCs), the cells distributed in the stromas of the body, are known for various properties including replication, the potential of various differentiations, the immune-related processes including inflammation. About two decades ago, these cells were shown to play relevant roles in the therapy of numerous diseases, dependent on their immune regulation and their release of cytokines and growth factors, with ensuing activation of favorable enzymes and processes. Such discovery induced great increase of their investigation. Soon thereafter, however, it became clear that therapeutic actions of MSCs are risky, accompanied by serious drawbacks and defects. MSC therapy has been therefore reduced to a few diseases, replaced for the others by their extracellular vesicles, the MSC-EVs. The latter vesicles recapitulate most therapeutic actions of MSCs, with equal or even better efficacies and without the serious drawbacks of the parent cells. In addition, MSC-EVs are characterized by many advantages, among which are their heterogeneities dependent on the stromas of origin, the alleviation of cell aging, the regulation of immune responses and inflammation. Here we illustrate the MSC-EV therapeutic effects, largely mediated by specific miRNAs, covering various diseases and pathological processes occurring in the bones, heart and vessels, kidney, and brain. MSC-EVs operate also on the development of cancers and on COVID-19, where they alleviate the organ lesions induced by the virus. Therapy by MSC-EVs can be improved by combination of their innate potential to engineering processes inducing precise targeting and transfer of drugs. The unique properties of MSC-EVs explain their intense studies, carried out with extraordinary success. Although not yet developed to clinical practice, the perspectives for proximal future are encouraging.

scholarly journals Bioactive Phenolic Compounds from Indian Medicinal Plants for Pharmaceutical and Medical Aspects

2021 ◽  
Author(s):  
Bhanu Kumar ◽  
Ankita Misra ◽  
Sharad Srivastava
Keyword(s):  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Modern Medicine ◽  
The Body ◽  
Human Welfare ◽  
Therapeutic Benefits ◽  
Plant Constituents ◽  
Health And Disease ◽  
Tools And Techniques ◽  
Indian Medicinal Plants

Ayurveda is an age old system of medicine which utilizes thousands of medicinal plants, rich in secondary metabolites for their therapeutic benefits and phenolic compounds are important one. Plant phenolic compounds are one of the major group of phytomolecules having tremendous therapeutic and nutraceutical potential. Indian medicinal plants like Emblica, Terminalia spp., Withania, Tinospora etc. are some of the potential source of bioactive phenolics and had been used from ages in various Ayurvedic formulations and were scientifically validated too. In this contribution, a brief account of some common Indian medicinal plants rich in bioactive phenolics are summarized along with their therapeutic action on human health and disease. The vast array of phenolics in these plants makes them a suitable candidate for modern medicine, nutraceutical supplements, immuno-modulatory formulations etc. With the advent of modern separation tools and techniques, it is now possible to identify, isolate and purify desired phytoconstituents from plant extracts. This further opens the avenues of utilizing medicinal plants or plant constituents/metabolites as super food for strengthening the body and maintaining the healthy work-life balance. The need of the hour is to identify therapeutically potential phenolics rich plants and development of herbal formulations for human welfare.

scholarly journals More than Nutrition: Therapeutic Potential of Breast Milk-Derived Exosomes in Cancer

2020 ◽  
Vol 21 (19) ◽  
pp. 7327
Author(s):  
Ki-Uk Kim ◽  
Wan-Hoon Kim ◽  
Chi Hwan Jeong ◽  
Dae Yong Yi ◽  
Hyeyoung Min
Keyword(s):  
Breast Milk ◽  
Therapeutic Potential ◽  
Critical Role ◽  
Drug Carriers ◽  
Enzymatic Digestion ◽  
Lymphocytic Leukemia ◽  
Human Breast Milk ◽  
Exosomal Mirnas ◽  
Acidic Conditions ◽  
Low Prevalence

Human breast milk (HBM) is an irreplaceable source of nutrition for early infant growth and development. Breast-fed children are known to have a low prevalence and reduced risk of various diseases, such as necrotizing enterocolitis, gastroenteritis, acute lymphocytic leukemia, and acute myeloid leukemia. In recent years, HBM has been found to contain a microbiome, extracellular vesicles or exosomes, and microRNAs, as well as nutritional components and non-nutritional proteins, including immunoregulatory proteins, hormones, and growth factors. Especially, the milk-derived exosomes exert various physiological and therapeutic function in cell proliferation, inflammation, immunomodulation, and cancer, which are mainly attributed to their cargo molecules such as proteins and microRNAs. The exosomal miRNAs are protected from enzymatic digestion and acidic conditions, and play a critical role in immune regulation and cancer. In addition, the milk-derived exosomes are developed as drug carriers for delivering small molecules and siRNA to tumor sites. In this review, we examined the various components of HBM and their therapeutic potential, in particular of exosomes and microRNAs, towards cancer.

scholarly journals Breast Milk, a Source of Beneficial Microbes and Associated Benefits for Infant Health

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1039 ◽  
Author(s):  
Katríona E. Lyons ◽  
C. Anthony Ryan ◽  
Eugene M. Dempsey ◽  
R. Paul Ross ◽  
Catherine Stanton
Keyword(s):  
Mammary Gland ◽  
Breast Milk ◽  
Human Milk ◽  
Infant Health ◽  
Gastrointestinal Disease ◽  
Newborn Infants ◽  
Human Breast Milk ◽  
Microbial Composition ◽  
Beneficial Microbes ◽  
Health And Nutrition

Human breast milk is considered the optimum feeding regime for newborn infants due to its ability to provide complete nutrition and many bioactive health factors. Breast feeding is associated with improved infant health and immune development, less incidences of gastrointestinal disease and lower mortality rates than formula fed infants. As well as providing fundamental nutrients to the growing infant, breast milk is a source of commensal bacteria which further enhance infant health by preventing pathogen adhesion and promoting gut colonisation of beneficial microbes. While breast milk was initially considered a sterile fluid and microbes isolated were considered contaminants, it is now widely accepted that breast milk is home to its own unique microbiome. The origins of bacteria in breast milk have been subject to much debate, however, the possibility of an entero-mammary pathway allowing for transfer of microbes from maternal gut to the mammary gland is one potential pathway. Human milk derived strains can be regarded as potential probiotics; therefore, many studies have focused on isolating strains from milk for subsequent use in infant health and nutrition markets. This review aims to discuss mammary gland development in preparation for lactation as well as explore the microbial composition and origins of the human milk microbiota with a focus on probiotic development.

scholarly journals The Protective Effect of Exercise in Neurodegenerative Diseases: The Potential Role of Extracellular Vesicles

Cells ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 2182 ◽  
Author(s):  
Oliver K Fuller ◽  
Martin Whitham ◽  
Suresh Mathivanan ◽  
Mark A Febbraio
Keyword(s):  
Physical Activity ◽  
Extracellular Vesicles ◽  
Potential Role ◽  
The Body ◽  
Therapeutic Effects ◽  
Health And Wellbeing ◽  
Systemic Factors ◽  
Diabetes And Obesity

Physical activity has systemic effects on the body, affecting almost every organ. It is important not only for general health and wellbeing, but also in the prevention of diseases. The mechanisms behind the therapeutic effects of physical activity are not completely understood; however, studies indicate these benefits are not confined to simply managing energy balance and body weight. They also include systemic factors which are released into the circulation during exercise and which appear to underlie the myriad of benefits exercise can elicit. It was shown that along with a number of classical cytokines, active tissues also engage in inter-tissue communication via extracellular vesicles (EVs), specifically exosomes and other small EVs, which are able to deliver biomolecules to cells and alter their metabolism. Thus, EVs may play a role in the acute and systemic adaptations that take place during and after physical activity, and may be therapeutically useful in the treatment of a range of diseases, including metabolic disorders such as type 2 diabetes and obesity; and the focus of this review, neurological disorders such as Alzheimer’s disease.

scholarly journals Extracellular vesicles in diagnosis and therapy of kidney diseases

2016 ◽  
Vol 311 (5) ◽  
pp. F844-F851 ◽  
Author(s):  
Wei Zhang ◽  
Xiangjun Zhou ◽  
Hao Zhang ◽  
Qisheng Yao ◽  
Yutao Liu ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Cross Talk ◽  
Cultured Cells ◽  
Therapeutic Potential ◽  
Kidney Diseases ◽  
Cell Communication ◽  
Therapeutic Effects ◽  
Glomerular Diseases ◽  
The Cross

Extracellular vesicles (EV) are endogenously produced, membrane-bound vesicles that contain various molecules. Depending on their size and origins, EVs are classified into apoptotic bodies, microvesicles, and exosomes. A fundamental function of EVs is to mediate intercellular communication. In kidneys, recent research has begun to suggest a role of EVs, especially exosomes, in cell-cell communication by transferring proteins, mRNAs, and microRNAs to recipient cells as nanovectors. EVs may mediate the cross talk between various cell types within kidneys for the maintenance of tissue homeostasis. They may also mediate the cross talk between kidneys and other organs under physiological and pathological conditions. EVs have been implicated in the pathogenesis of both acute kidney injury and chronic kidney diseases, including renal fibrosis, end-stage renal disease, glomerular diseases, and diabetic nephropathy. The release of EVs with specific molecular contents into urine and plasma may be useful biomarkers for kidney disease. In addition, EVs produced by cultured cells may have therapeutic effects for these diseases. However, the role of EVs in kidney diseases is largely unclear, and the mechanism underlying EV production and secretion remains elusive. In this review, we introduce the basics of EVs and then analyze the present information about the involvement, diagnostic value, and therapeutic potential of EVs in major kidney diseases.

scholarly journals Dental stem cell-derived extracellular vesicles as promising therapeutic agents in the treatment of diseases

2022 ◽  
Vol 14 (1) ◽  
Author(s):  
Ye Li ◽  
Xu Duan ◽  
Yinxue Chen ◽  
Bingyun Liu ◽  
Gang Chen
Keyword(s):  
Stem Cells ◽  
Extracellular Vesicles ◽  
Therapeutic Potential ◽  
In Vivo Studies ◽  
Therapeutic Effects ◽  
Positive Effects ◽  
Direct Cell ◽  
Action Modes ◽  
Dental Stem Cell

AbstractDental stem cells (DSCs), an important source of mesenchymal stem cells (MSCs), can be easily obtained by minimally invasive procedures and have been used for the treatment of various diseases. Classic paradigm attributed the mechanism of their therapeutic action to direct cell differentiation after targeted migration, while contemporary insights into indirect paracrine effect opened new avenues for the mystery of their actual low engraftment and differentiation ability in vivo. As critical paracrine effectors, DSC-derived extracellular vesicles (DSC-EVs) are being increasingly linked to the positive effects of DSCs by an evolving body of in vivo studies. Carrying bioactive contents and presenting therapeutic potential in certain diseases, DSC-EVs have been introduced as promising treatments. Here, we systematically review the latest in vivo evidence that supports the therapeutic effects of DSC-EVs with mechanistic studies. In addition, current challenges and future directions for the clinical translation of DSC-EVs are also highlighted to call for more attentions to the (I) distinguishing features of DSC-EVs compared with other types of MSC-EVs, (II) heterogeneity among different subtypes of DSC-derived EVs, (III) action modes of DSC-EVs, (IV) standardization for eligible DSC-EVs and (V) safety guarantee for the clinical application of DSC-EVs. The present review would provide valuable insights into the emerging opportunities of DSC-EVs in future clinical applications.

scholarly journals Therapeutic Effects of Hypoxic and Pro-Inflammatory Priming of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Inflammatory Arthritis

2021 ◽  
Vol 23 (1) ◽  
pp. 126
Author(s):  
Alasdair G. Kay ◽  
Kane Treadwell ◽  
Paul Roach ◽  
Rebecca Morgan ◽  
Rhys Lodge ◽  
...  
Keyword(s):  
T Cell ◽  
Extracellular Vesicles ◽  
Inflammatory Responses ◽  
Therapeutic Potential ◽  
Histopathological Analysis ◽  
Therapeutic Effects ◽  
Synovial Joint ◽  
Paracrine Signalling

Mesenchymal stem cells (MSCs) immunomodulate inflammatory responses through paracrine signalling, including via secretion of extracellular vesicles (EVs) in the cell secretome. We evaluated the therapeutic potential of MSCs-derived small EVs in an antigen-induced model of arthritis (AIA). EVs isolated from MSCs cultured normoxically (21% O2, 5% CO2), hypoxically (2% O2, 5% CO2) or with a pro-inflammatory cytokine cocktail were applied into the AIA model. Disease pathology was assessed post-arthritis induction through swelling and histopathological analysis of synovial joint structure. Activated CD4+ T cells from healthy mice were cultured with EVs or MSCs to assess deactivation capabilities prior to application of standard EVs in vivo to assess T cell polarisation within the immune response to AIA. All EVs treatments reduced knee-joint swelling whilst only normoxic and pro-inflammatory primed EVs improved histopathological outcomes. In vitro culture with EVs did not achieve T cell deactivation. Polarisation towards CD4+ helper cells expressing IL17a (Th17) was reduced when normoxic and hypoxic EV treatments were applied in vitro. Normoxic EVs applied into the AIA model reduced Th17 polarisation and improved Regulatory T cell (Treg):Th17 homeostatic balance. Normoxic EVs present the optimal strategy for broad therapeutic benefit. EVs present an effective novel technology with the potential for cell-free therapeutic translation.

scholarly journals Challenges in the Isolation and Proteomic Analysis of Cancer Exosomes—Implications for Translational Research

Proteomes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 22 ◽  
Author(s):  
Jadwiga Jablonska ◽  
Monika Pietrowska ◽  
Sonja Ludwig ◽  
Stephan Lang ◽  
Basant Kumar Thakur
Keyword(s):  
Extracellular Vesicles ◽  
Proteome Analysis ◽  
The Body ◽  
Cell Of Origin ◽  
Proteomics Data ◽  
Therapeutic Approaches ◽  
Protein Targets ◽  
Isolation Methods ◽  
Health And Disease ◽  
Novel Protein

Exosomes belong to the group of extracellular vesicles (EVs) that derive from various cell populations and mediate intercellular communication in health and disease. Like hormones or cytokines, exosomes released by cells can play a potent role in the communication between the cell of origin and distant cells in the body to maintain homeostatic or pathological processes, including tumorigenesis. The nucleic acids, and lipid and protein cargo present in the exosomes are involved in a myriad of carcinogenic processes, including cell proliferation, tumor angiogenesis, immunomodulation, and metastasis formation. The ability of exosomal proteins to mediate direct functions by interaction with other cells qualifies them as tumor-specific biomarkers and targeted therapeutic approaches. However, the heterogeneity of plasma-derived exosomes consistent of (a) exosomes derived from all kinds of body cells, including cancer cells and (b) contamination of exosome preparation with other extracellular vesicles, such as apoptotic bodies, makes it challenging to obtain solid proteomics data for downstream clinical application. In this manuscript, we review these challenges beginning with the choice of different isolation methods, through the evaluation of obtained exosomes and limitations in the process of proteome analysis of cancer-derived exosomes to identify novel protein targets with functional impact in the context of translational oncology.

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