scholarly journals Conversion of Starchy Waste Streams into Polyhydroxyalkanoates Using Cupriavidus necator DSM 545

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1496
Author(s):  
Silvia Brojanigo ◽  
Elettra Parro ◽  
Tiziano Cazzorla ◽  
Lorenzo Favaro ◽  
Marina Basaglia ◽  
...  

Due to oil shortage and environmental problems, synthetic plastics have to be replaced by different biodegradable materials. A promising alternative could be polyhydroxyalkanoates (PHAs), and the low-cost abundant agricultural starchy by-products could be usefully converted into PHAs by properly selected and/or developed microbes. Among the widely available starchy waste streams, a variety of residues have been explored as substrates, such as broken, discolored, unripe rice and white or purple sweet potato waste. Cupriavidus necator DSM 545, a well-known producer of PHAs, was adopted in a simultaneous saccharification and fermentation (SSF) process through an optimized dosage of the commercial amylases cocktail STARGEN™ 002. Broken rice was found to be the most promising carbon source with PHAs levels of up to 5.18 g/L. This research demonstrates that rice and sweet potato waste are low-cost feedstocks for PHAs production, paving the way for the processing of other starchy materials into bioplastics.

2014 ◽  
Vol 80 (20) ◽  
pp. 6458-6464 ◽  
Author(s):  
Lixiang Li ◽  
Chao Chen ◽  
Kun Li ◽  
Yu Wang ◽  
Chao Gao ◽  
...  

ABSTRACT2,3-Butanediol (2,3-BD) is an important starting material for the manufacture of bulk chemicals. For efficient and large-scale production of 2,3-BD through fermentation, low-cost substrates are required. One such substrate, inulin, is a polydisperse fructan found in a wide variety of plants. In this study, a levanase with high inulinase activity and high pH and temperature stability was identified inBacillus licheniformisstrain ATCC 14580.B. licheniformisstrain ATCC 14580 was found to efficiently produce 2,3-BD from fructose at 50°C. Then, the levanase was used for simultaneous saccharification and fermentation (SSF) of inulin to 2,3-BD. A fed-batch SSF yielded 103.0 g/liter 2,3-BD in 30 h, with a high productivity of 3.4 g/liter · h. The results suggest that the SSF process developed with the thermophilicB. licheniformisstrain used might be a promising alternative for efficient 2,3-BD production from the favorable substrate inulin.


Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1048
Author(s):  
Micaela Vannini ◽  
Paola Marchese ◽  
Laura Sisti ◽  
Andrea Saccani ◽  
Taihua Mu ◽  
...  

With the aim to fully exploit the by-products obtained after the industrial extraction of starch from sweet potatoes, a cascading approach was developed to extract high-value molecules, such as proteins and pectins, and to valorize the solid fraction, rich in starch and fibrous components. This fraction was used to prepare new biocomposites designed for food packaging applications. The sweet potato residue was added to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in various amounts up to 40 wt % by melt mixing, without any previous treatment. The composites are semicrystalline materials, characterized by thermal stability up to 260 °C. For the composites containing up to 10 wt % of residue, the tensile strength remains over 30 MPa and the strain stays over 3.2%. A homogeneous dispersion of the sweet potato waste into the bio-polymeric matrix was achieved but, despite the presence of hydrogen bond interactions between the components, a poor interfacial adhesion was detected. Considering the significant percentage of sweet potato waste used, the biocomposites obtained show a low economic and environmental impact, resulting in an interesting bio-alternative to the materials commonly used in the packaging industry. Thus, according to the principles of a circular economy, the preparation of the biocomposites closes the loop of the complete valorization of sweet potato products and by-products.


Author(s):  
Sheila Dwi Shilviana ◽  
Ni Luh Suriani ◽  
I Ketut Sundra

Compost is an organic fertilizer made from recycled organic materials. Fertilizers can be made from organic waste or unused organic waste. Purple sweet potato waste can be reused into compost. The purpose of this study was to examine the effectiveness of purple sweet potato waste compost inoculated with Trichoderma harzianum in increasing the production of pakchoi and to determine the treatment of purple sweet potato waste compost to increase the production of pakchoi. The research method used is the experimental method. The experimental design used was RAK (Randomized Block Design) with 1 factor. The experiment consisted of 4 treatments of purple sweet potato compost 25 (B) g, 50 g (C), 75 g (D), 150 g (E), and 1 control (A), and was repeated 5 times. The data analysis technique used analysis of variance (ANOVA). Parameters observed were the number of leaves, plant height, leaf width, plant wet weight, plant dry weight, and root length. The results showed that the application of purple sweet potato compost affected increasing the growth and production of pakchoi. The right treatment in increasing the growth of pakchoi is treatment 150 g or treatment E. Treatment 150 g (E) can increase the number of leaves (13.13), plant height (16.32 cm), leaf width (4.88 cm), wet weight (16.60 g), dry weight (6.35 g), and root length (15.80 cm).


2014 ◽  
Vol 32 (No. 5) ◽  
pp. 485-492 ◽  
Author(s):  
J. Nowak ◽  
K. Szambelan ◽  
W. Nowak

We assessed the composition of volatile by-products in raw spirits obtained from moist corn fermentation. The average moisture value of the researched samples was 35.4%. A comparative research was conducted applying simultaneous saccharification and fermentation (SSF) process with Saccharomyces cerevisiae. The analysis characterised corn grains after three and six months of storage in aerobic and anaerobic conditions. The yield of ethanol fermentation was 42.43 and 39.12 l/100 kg dry matter after three and six months of storage, respectively. The storage of moist grain resulted in the reduction of higher alcohols content in the raw spirits. It was observed that the esters concentration decreased after three, but increased after six months of raw material storage. A significant increase occurred in the quantity of aldehydes detected only after three months of the corn storage. The results show that the application of SSF technology to moist corn, allows the production of bioethanol with quality comparable to that obtained with dried grain.  


2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins


2019 ◽  
Author(s):  
Chem Int

The removal of Cd(II) and Pb(II) ions from aqueous medium was studied using potato peels biomass. The adsorption process was evaluated using Atomic Absorption Spectrophotometer (AAS). The Vibrational band of the potato peels was studied using Fourier Transform Infrared Spectroscopy (FTIR). The adsorption process was carried out with respect to concentration, time, pH, particle size and the thermodynamic evaluation of the process was carried at temperatures of 30, 40, 50 and 60(0C), respectively. The FTIR studies revealed that the potato peels was composed of –OH, -NH, –C=N, –C=C and –C-O-C functional groups. The optimum removal was obtained at pH 8 and contact time of 20 min. The adsorption process followed Freundlich adsorption and pseudo second-order kinetic models with correlation coefficients (R2) greater than 0.900. The equilibrium adsorption capacity showed that Pb(II) ion was more adsorbed on the surface of the potato peels biomass versus Cd (II) ion (200.91 mg/g &gt; 125.00 mg/g). The thermodynamic studies indicated endothermic, dissociative mechanism and spontaneous adsorption process. This study shows that sweet potato peels is useful as a low-cost adsorbent for the removal of Cd(II) and Pb(II) ions from aqueous medium.


2018 ◽  
Vol 8 (2) ◽  
pp. 144
Author(s):  
Ria Afrianti

This study aims to determine the effect giving of ethylacetate fraction of leather  purple sweet potato (Ipomoea batatas (L.) Lam, on levels of malondialdehyde (MDA) serum in mice hyperglicemia were induced with streptozocin dose of 50 mg/kgBW. Mice were divided into 5 groups, each group consisting of 3 tails, group I is a negative control, group II is a positive control, group III,IV and V is given ethylacetate fraction a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW. Ethyl Acetate Fraction leather purple sweet potato given orally for 15 days after the animal is declared hyperglicemia and measurement of blood glucose levels on 5, 10, and 15 day after giving test preparation in animal experiments. On the 16 day throughout the mice were taken serum levels measured malondialdehid. The statistical analysis results showed that giving of ethyl acetate fraction of leather purple sweet potato at a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW can lower blood glucose levels in mice hyperglycemia significantly (p<0.05). Malondialdehid levels on average in each group is 1.35 nmol/ml, 3.00 nmol/ml, 2.72 nmol/ml, 2.20 nmol/ml and 2.61 nmol/ml, the results of statistical analysis showed a decrease in melondialdehid serum levels were significantly (p<0.05), where a dose of 300 mg/kgBW is an effective dose for lowering blood glucose levels followed by decreased levels of malondialdehid which give effect approaching negative control.


2020 ◽  
Vol 16 (4) ◽  
pp. 455-461
Author(s):  
Gabriela M. Baia ◽  
Otniel Freitas-Silva ◽  
Murillo F. Junior

Fruits and vegetables are foods that come into contact with various types of microorganisms from planting to their consumption. A lack or poor sanitation of these products after harvest can cause high losses due to deterioration and/ or pathogenic microorganisms. There are practically no post-harvest fungicides or bactericides with a broad spectrum of action that have no toxic residual effects and are safe. However, to minimize such problems, the use of sanitizers is an efficient device against these microorganisms. Chlorine is the most prevalent sanitizing agent because of its broad spectrum, low cost and well-established practices. However, the inevitable formation of disinfection by-products, such as trihalomethanes (THMs) and haloacetic acids (HAAs), is considered one of the main threats to food safety. Alternative sanitizers, such as chlorine dioxide (ClO2) and ozone, are becoming popular as a substitute for traditional post-harvest treatments. Thus, this review addresses the use of chlorine, chlorine dioxide and ozone emphasizing aspects, such as usage, safe application, spectrum of action and legislation. In order to ensure the quality and safety of final products, the adoption of well-prepared sanitation and sanitation programs for post-harvest fruits and vegetables is essential.


2019 ◽  
Vol 284 ◽  
pp. 286-292 ◽  
Author(s):  
Xinfei Chen ◽  
Xiaowei Peng ◽  
Xiaoqian Ma ◽  
Jingjing Wang

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