scholarly journals Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2661
Author(s):  
Ana Mora-Boza ◽  
Elena López-Ruiz ◽  
María Luisa López-Donaire ◽  
Gema Jiménez ◽  
María Rosa Aguilar ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Polymer Network ◽  
Physicochemical Characterization ◽  
Human Mesenchymal Stem Cells ◽  
Ionic Crosslinking ◽  
Crosslinking Process ◽  
Potential Applications ◽  
Biological Performance

In the present study, semi- and interpenetrated polymer network (IPN) systems based on hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker, glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized and evaluated as potential supports for tissue engineering. Semi- and IPN systems showed significant differences between them regarding composition, morphology, and mechanical properties after physicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retention and mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPN membranes. The biological performance was evaluated on primary human mesenchymal stem cells (hMSCs) and the systems revealed no cytotoxic effect. The excellent biocompatibility of the systems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces. Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN, which suggested that these polymeric membranes could be proposed as an effective promoter system of tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranes can provide a stable and amenable environment for hMSCs support and growth with potential applications in the biomedical field.

scholarly journals A New Generation of Electrospun Fibers Containing Bioactive Glass Particles for Wound Healing

Materials ◽  
2020 ◽  
Vol 13 (24) ◽  
pp. 5651
Author(s):  
Rachele Sergi ◽  
Valeria Cannillo ◽  
Aldo R. Boccaccini ◽  
Liliana Liverani
Keyword(s):  
Mechanical Properties ◽  
Wound Healing ◽  
Bioactive Glass ◽  
Flexible Structures ◽  
Biological Properties ◽  
Cellular Viability ◽  
Bioactive Glasses ◽  
Electrospinning Technique ◽  
Potential Applications ◽  
Biological Performance

Chitosan fibers blended with polyethylene oxide (CHIT_PEO) and crosslinked with genipin were fabricated by electrospinning technique. Subsequently, CHIT_PEO bioactive glass composite electrospun mats were fabricated with the aim to achieve flexible structures with adequate mechanical properties and improved biological performance respect to CHIT_PEO fibers, for potential applications in wound healing. Three different compositions of bioactive glasses (BG) were selected and investigated: 45S5 BG, a Sr and Mg containing bioactive glass (BGMS10) and a Zn-containing bioactive glass (BGMS_2Zn). Particulate BGs (particles size < 20 μm) were separately added to the starting CHIT_PEO solution before electrospinning. The two recently developed bioactive glasses (BGMS10 and BGMS_2Zn) showed very promising biological properties in terms of bioactivity and cellular viability; thus, such compositions were added for the first time to CHIT_PEO solution to fabricate composite electrospun mats. The incorporation of bioactive glass particles and their distribution into CHIT_PEO fibers were assessed by SEM and FTIR analyses. Furthermore, CHIT_PEO composite electrospun mats showed improved mechanical properties in terms of Young’s Modulus compared to neat CHIT_PEO fibers; on the contrary, the values of tensile strain at break (%) were comparable. Biological performance in terms of cellular viability was investigated by means of WST-8 assay and CHIT_PEO composite electrospun mats showed cytocompatibility and the desired cellular viability.

scholarly journals Biomimicry in Bio-Manufacturing: Developments in Melt Electrospinning Writing Technology Towards Hybrid Biomanufacturing

2019 ◽  
Vol 9 (17) ◽  
pp. 3540 ◽  
Author(s):  
Ferdows Afghah ◽  
Caner Dikyol ◽  
Mine Altunbek ◽  
Bahattin Koc
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Future Perspective ◽  
Melt Electrospinning ◽  
Wide Range ◽  
Challenges And Opportunities ◽  
Potential Applications ◽  
Homogeneous Cell

Melt electrospinning writing has been emerged as a promising technique in the field of tissue engineering, with the capability of fabricating controllable and highly ordered complex three-dimensional geometries from a wide range of polymers. This three-dimensional (3D) printing method can be used to fabricate scaffolds biomimicking extracellular matrix of replaced tissue with the required mechanical properties. However, controlled and homogeneous cell attachment on melt electrospun fibers is a challenge. The combination of melt electrospinning writing with other tissue engineering approaches, called hybrid biomanufacturing, has introduced new perspectives and increased its potential applications in tissue engineering. In this review, principles and key parameters, challenges, and opportunities of melt electrospinning writing, and particularly, recent approaches and materials in this field are introduced. Subsequently, hybrid biomanufacturing strategies are presented for improved biological and mechanical properties of the manufactured porous structures. An overview of the possible hybrid setups and applications, future perspective of hybrid processes, guidelines, and opportunities in different areas of tissue/organ engineering are also highlighted.

scholarly journals Engineering a macroporous fibrin-based sequential interpenetrating polymer network for dermal tissue engineering

2020 ◽  
Vol 8 (24) ◽  
pp. 7106-7116
Author(s):  
Olfat Gsib ◽  
Loek J. Eggermont ◽  
Christophe Egles ◽  
Sidi A. Bencherif
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Biological Activity ◽  
Polymer Network ◽  
Interpenetrating Polymer Network ◽  
Dermal Tissue

Macroporous and mechanically reinforced sequential IPN hydrogels combine the biological activity of fibrin with the robust mechanical properties of PEG to generate advanced scaffolds for dermal tissue engineering.

Preparation and Characterization of Poly(Vinyl Alcohol)(PVA)/Hydroxyapatite (HA) Nanofibrous Scaffolds

2011 ◽  
Vol 284-286 ◽  
pp. 459-463 ◽  
Author(s):  
Yuan Yuan Qi ◽  
Bin Liu ◽  
Xing Bin Yan
Keyword(s):  
Mechanical Properties ◽  
Drug Delivery ◽  
Tissue Engineering ◽  
Tensile Strength ◽  
Drug Delivery Systems ◽  
Poly Vinyl Alcohol ◽  
Elongation At Break ◽  
Nanofibrous Scaffolds ◽  
Potential Applications

Nanofibrous scaffolds of PVA and HA were prepared by electrospinning. SEM showed the scaffolds had porous nanofibrous morphology, and the diameter of the fibers was in the range of 200-1000 nm. FTIR and XRD showed the presence of HA in the scaffolds. The mechanical properties of the scaffolds changed by the adding content of HA. For the nanoscaffolds with 2wt % HA, the ultimate tensile strength and the elongation at break was 7.5 MPa and 17%. The PVA/HA nanoscaffolds prepared by electrospinning indicated good properties, and had a potential applications in bone tissue engineering and drug delivery systems.

scholarly journals Crosslinking of hydrophilic polymers using polyperoxides

2020 ◽  
Vol 298 (12) ◽  
pp. 1699-1713
Author(s):  
Solomiia Borova ◽  
Victor Tokarev ◽  
Philipp Stahlhut ◽  
Robert Luxenhofer
Keyword(s):  
Mechanical Properties ◽  
Mechanical Performance ◽  
Soft Materials ◽  
Hydrophilic Polymers ◽  
Medical Applications ◽  
Crucial Importance ◽  
New Materials ◽  
Toxic Compounds ◽  
Crosslinking Process ◽  
Potential Applications

Abstract Hydrogels that can mimic mechanical properties and functions of biological tissue have attracted great interest in tissue engineering and biofabrication. In these fields, new materials and approaches to prepare hydrogels without using toxic starting materials or materials that decompose into toxic compounds remain to be sought after. Here, we report the crosslinking of commercial, unfunctionalized hydrophilic poly(2-ethyl-2-oxazoline) using peroxide copolymers in their melt. The influence of temperature, peroxide copolymer concentration, and duration of the crosslinking process has been investigated. The method allows to create hydrogels from unfunctionalized polymers in their melt and to control the mechanical properties of the resulting materials. The design of hydrogels with a suitable mechanical performance is of crucial importance in many existing and potential applications of soft materials, including medical applications.

scholarly journals A Hyaluronic Acid Based Injectable Hydrogel Formed via Photo-Crosslinking Reaction and Thermal-Induced Diels-Alder Reaction for Cartilage Tissue Engineering

Polymers ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 949 ◽  
Author(s):  
Gang Wang ◽  
Xiaodong Cao ◽  
Hua Dong ◽  
Lei Zeng ◽  
Chenxi Yu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Cell Activity ◽  
Cartilage Tissue Engineering ◽  
Diels Alder ◽  
Cartilage Tissue ◽  
Good Prospect ◽  
Crosslinking Reaction ◽  
Injectable Hydrogel

A hyaluronic acid (HA) based injectable hydrogel with gradually increasing mechanical properties was synthesized via photo-crosslinking reaction and thermal-induced Diels-Alder (DA) reaction. The injectable hydrogel can quickly gelate within 30 s by photo-crosslinking of HA-furan under the catalysis of lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP). This injectable property is beneficial to keep the encapsulated cell activity and convenient for clinical operation. And the mechanical properties can be control from 4.86 to 10.66 kPa by exposure time. Then, the thermal-induced DA click chemistry further occurs between furan groups and maleimide groups which gradually promoted the crosslinking density of the injectable hydrogel. The mechanical properties of the injectable hydrogel can be promoted to 21 kPa. ATDC-5 cells were successfully encapsulated in the injectable hydrogel and showed good activity. All the results suggested that the injectable hydrogel with gradually increasing mechanical properties formed by photo-crosslinking reaction and thermal-induced DA reaction has a good prospect of application in cartilage tissue engineering.

Hyaluronic acid/EDC/NHS-crosslinked green electrospun silk fibroin nanofibrous scaffolds for tissue engineering

RSC Advances ◽  
2016 ◽  
Vol 6 (102) ◽  
pp. 99720-99728 ◽  
Author(s):  
Xingxing Yang ◽  
Xiaoyun Wang ◽  
Fan Yu ◽  
Linlin Ma ◽  
Xiaohan Pan ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Soft Tissue ◽  
Silk Fibroin ◽  
Nanofibrous Scaffolds ◽  
Soft Tissue Engineering

The mechanical properties of SF nanofibrous matrices were enhanced through crosslinking with HA/EDC/NHS for soft tissue engineering.

Polyurethane/chitosan/hyaluronic acid scaffolds: providing an optimum environment for fibroblast growth

2020 ◽  
Vol 29 (10) ◽  
pp. 586-596
Author(s):  
Seyedeh-Sara Hashemi ◽  
Seyedeh-Somayeh Rajabi ◽  
Reza Mahmoudi ◽  
Amir Ghanbari ◽  
Kazem Zibara ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Contact Angle ◽  
Tensile Strength ◽  
Hyaluronic Acid ◽  
Cell Viability ◽  
Uniform Structure ◽  
Fibroblast Cells ◽  
Fibroblast Growth ◽  
The Mean

Objective: Biodegradable polymers can replace damaged tissue components using tissue engineering techniques. The objective of this study is to determine an optimum environment for polymer scaffolds to improve the proliferation of fibroblast cells capable of wound repair. Method: In this study, the addition of polysaccharides, such as chitosan (CH) or hyaluronic acid (HA), to a polyurethane (PU) polymer was evaluated using different methods to determine if they affect scaffold morphology and cell activity of fibroblasts prepared from human foreskin tissues. Mechanical properties, such as tensile strength, contact angle and swelling test, were used to check the physical and mechanical properties of the scaffold. Fibroblast growth was also measured at 24, 48 and 72 hours. Results: Scanning electron microscopy (SEM) determined that a 3:1 ratio of PU/CH scaffold, developed by electrospinning, allowed the formation of a uniform structure in scaffold fibres. Physical mechanical tests showed that PU electrospun scaffolds were not modified by the addition of CH. The mean stretch and mean water absorption increased significantly using the PU/CH scaffold, compared with the PU scaffold. However, the mean tensile strength and the mean contact angle, used to study space and porosity, did not differ between scaffolds. Fourier transform infrared spectroscopy confirmed the functional groups (–OH, –NH and –C=O) in the PU/CH scaffold, compared with PU or CH chemical structures alone. HA was then added to CH and PU/CH scaffolds to evaluate the growth of fibroblast cells. Results showed that cell viability and the number of cells, using MTT and trypan blue exclusion assay, respectively, increased significantly at 24, 48 and 72 hours of culture in PU/CH/HA scaffold compared to HA, CH/HA, and PU/HA. Moreover, PU/HA at 48 and 72 hours also increased cell viability and cell numbers compared to HA and CH/HA scaffolds. However, scaffolds at 72 hours had limited space for cell growth. Moreover, SEM data demonstrated that fibroblasts were able to proliferate, penetrate, migrate and survive on PU/HA and PU/CH/HA three-dimensional scaffolds, especially during the first 48 hours. Furthermore, 4′,6-diamidino-2-phenylindole (DAPI) staining confirmed that fibroblasts could penetrate PU scaffolds and showed higher cell viability and lower cellular damage in PU/CH/HA, compared to CH/HA and PU/HA scaffolds. Finally, flow cytometry using CD90 and CD105 surface markers revealed that >90% of cells isolated from the human dermis were fibroblasts. Conclusion: In summary, PU/HA and PU/CH/HA scaffolds were found to be biocompatible and provided a suitable environment for the growth and proliferation of fibroblasts, which filled and covered all pores between the fibres. The new scaffold used in this study, made of synthetic and natural polymers, is a good candidate for applications in tissue engineering. It is therefore recommended to use PU in grafts or in wound dressing.

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