scholarly journals A New Generation of Electrospun Fibers Containing Bioactive Glass Particles for Wound Healing

Materials ◽  
2020 ◽  
Vol 13 (24) ◽  
pp. 5651
Author(s):  
Rachele Sergi ◽  
Valeria Cannillo ◽  
Aldo R. Boccaccini ◽  
Liliana Liverani
Keyword(s):  
Mechanical Properties ◽  
Wound Healing ◽  
Bioactive Glass ◽  
Flexible Structures ◽  
Biological Properties ◽  
Cellular Viability ◽  
Bioactive Glasses ◽  
Electrospinning Technique ◽  
Potential Applications ◽  
Biological Performance

Chitosan fibers blended with polyethylene oxide (CHIT_PEO) and crosslinked with genipin were fabricated by electrospinning technique. Subsequently, CHIT_PEO bioactive glass composite electrospun mats were fabricated with the aim to achieve flexible structures with adequate mechanical properties and improved biological performance respect to CHIT_PEO fibers, for potential applications in wound healing. Three different compositions of bioactive glasses (BG) were selected and investigated: 45S5 BG, a Sr and Mg containing bioactive glass (BGMS10) and a Zn-containing bioactive glass (BGMS_2Zn). Particulate BGs (particles size < 20 μm) were separately added to the starting CHIT_PEO solution before electrospinning. The two recently developed bioactive glasses (BGMS10 and BGMS_2Zn) showed very promising biological properties in terms of bioactivity and cellular viability; thus, such compositions were added for the first time to CHIT_PEO solution to fabricate composite electrospun mats. The incorporation of bioactive glass particles and their distribution into CHIT_PEO fibers were assessed by SEM and FTIR analyses. Furthermore, CHIT_PEO composite electrospun mats showed improved mechanical properties in terms of Young’s Modulus compared to neat CHIT_PEO fibers; on the contrary, the values of tensile strain at break (%) were comparable. Biological performance in terms of cellular viability was investigated by means of WST-8 assay and CHIT_PEO composite electrospun mats showed cytocompatibility and the desired cellular viability.

Hybrid Bioactive Glass-Polyvinyl Alcohol Prepared by Sol-Gel

2008 ◽  
Vol 587-588 ◽  
pp. 62-66 ◽  
Author(s):  
Hermes S. Costa ◽  
Alexandra A.P. Mansur ◽  
Edel Figueiredo Barbosa-Stancioli ◽  
Marivalda Pereira ◽  
Herman S. Mansur
Keyword(s):  
Mechanical Properties ◽  
Polyvinyl Alcohol ◽  
Bioactive Glass ◽  
Sol Gel ◽  
Degree Of Hydrolysis ◽  
Bioactive Glasses ◽  
Composite Foams ◽  
Composition Ratios ◽  
Hybrid Scaffolds ◽  
Hydrolysis Of

Bioactive glasses are materials that have been used for the repair and reconstruction of diseased bone tissues, as they exhibit direct bonding with human bone tissues. However, bioactive glasses have low mechanical properties compared to cortical and cancellous bone. On the other hand, composite materials of biodegradable polymers with inorganic bioactive glasses are of particular interest to engineered scaffolds because they often show an excellent balance between strength and toughness and usually improved characteristics compared to their individual components. Composite bioactive glass-polyvinyl alcohol foams for use as scaffolds in tissue engineering were previously developed using the sol-gel route. The goal of this work was the synthesis of composite foams modified with higher amounts of PVA. Samples were characterized by morphological and chemical analysis. The mechanical behavior of the obtained materials was also investigated. The degree of hydrolysis of PVA, concentration of PVA solution and different PVA-bioactive glass composition ratios affect the synthesis procedure. Foams with up to 80 wt% polymer content were obtained. The hybrid scaffolds obtained exhibited macroporous structure with pore size varying from 50 to 600 µm and improved mechanical properties.

scholarly journals Effect of Melt-Derived Bioactive Glass Particles on the Properties of Chitosan Scaffolds

2019 ◽  
Vol 10 (3) ◽  
pp. 38 ◽  
Author(s):  
Hamasa Faqhiri ◽  
Markus Hannula ◽  
Minna Kellomäki ◽  
Maria Teresa Calejo ◽  
Jonathan Massera
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bioactive Glass ◽  
Pore Size ◽  
Glass Content ◽  
Micro Computed Tomography ◽  
Glass Composite ◽  
Composite Scaffolds ◽  
Bioactive Glasses

This study reports on the processing of three-dimensional (3D) chitosan/bioactive glass composite scaffolds. On the one hand, chitosan, as a natural polymer, has suitable properties for tissue engineering applications but lacks bioactivity. On the other hand, bioactive glasses are known to be bioactive and to promote a higher level of bone formation than any other biomaterial type. However, bioactive glasses are hard, brittle, and cannot be shaped easily. Therefore, in the past years, researchers have focused on the processing of new composites. Difficulties in reaching composite materials made of polymer (synthetic or natural) and bioactive glass include: (i) The high glass density, often resulting in glass segregation, and (ii) the fast bioactive glass reaction when exposed to moisture, leading to changes in the glass reactivity and/or change in the polymeric matrix. Samples were prepared with 5, 15, and 30 wt% of bioactive glass S53P4 (BonAlive ®), as confirmed using thermogravimetric analysis. MicrO–Computed tomography and optical microscopy revealed a flaky structure with porosity over 80%. The pore size decreased when increasing the glass content up to 15 wt%, but increased back when the glass content was 30 wt%. Similarly, the mechanical properties (in compression) of the scaffolds increased for glass content up to 15%, but decreased at higher loading. Ions released from the scaffolds were found to lead to precipitation of a calcium phosphate reactive layer at the scaffold surface. This is a first indication of the potential bioactivity of these materials. Overall, chitosan/bioactive glass composite scaffolds were successfully produced with pore size, machinability, and ability to promote a calcium phosphate layer, showing promise for bone tissue engineering and the mechanical properties can justify their use in non-load bearing applications.

scholarly journals A Comparison of Bioactive Glass Scaffolds Fabricated ‎by Robocasting from Powders Made by Sol–Gel and Melt-Quenching Methods

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 615
Author(s):  
Basam A. E. Ben-Arfa ◽  
Robert C. Pullar
Keyword(s):  
Mechanical Properties ◽  
Bioactive Glass ◽  
Bone Growth ◽  
Sol Gel ◽  
Glass Network ◽  
Silica Content ◽  
Bioactive Glasses ◽  
Phosphorous Content ◽  
High Silica ◽  
45S5 Bioglass

Bioactive glass scaffolds are used in bone and tissue biomedical implants, and there is great interest in their fabrication by additive manufacturing/3D printing techniques, such as robocasting. Scaffolds need to be macroporous with voids ≥100 m to allow cell growth and vascularization, biocompatible and bioactive, with mechanical properties matching the host tissue (cancellous bone for bone implants), and able to dissolve/resorb over time. Most bioactive glasses are based on silica to form the glass network, with calcium and phosphorous content for new bone growth, and a glass modifier such as sodium, the best known being 45S5 Bioglass®. 45S5 scaffolds were first robocast in 2013 from melt-quenched glass powder. Sol–gel-synthesized bioactive glasses have potential advantages over melt-produced glasses (e.g., greater porosity and bioactivity), but until recently were never robocast as scaffolds, due to inherent problems, until 2019 when high-silica-content sol–gel bioactive glasses (HSSGG) were robocast for the first time. In this review, we look at the sintering, porosity, bioactivity, biocompatibility, and mechanical properties of robocast sol–gel bioactive glass scaffolds and compare them to the reported results for robocast melt-quench-synthesized 45S5 Bioglass® scaffolds. The discussion includes formulation of the printing paste/ink and the effects of variations in scaffold morphology and inorganic additives/dopants.

Bioactive glasses in wound healing: hope or hype?

2017 ◽  
Vol 5 (31) ◽  
pp. 6167-6174 ◽  
Author(s):  
Shiva Naseri ◽  
William C. Lepry ◽  
Showan N. Nazhat
Keyword(s):  
Wound Healing ◽  
Soft Tissue ◽  
Tissue Regeneration ◽  
Tissue Repair ◽  
Great Promise ◽  
Mineralized Tissue ◽  
Bioactive Glasses ◽  
Soft Tissue Repair ◽  
Potential Applications

Bioactive glasses have long been investigated in mineralized tissue regeneration, but recently their potential applications in soft tissue repair, and in particular wound healing, have demonstrated great promise.

scholarly journals Porogen Effect of Bioactive Glass on Poly(L-lactide) Scaffolds: Evidences by Electron Microscopy

2008 ◽  
Vol 14 (S3) ◽  
pp. 65-66
Author(s):  
N.B. Barroca ◽  
A.L. Daniel-da-Silva ◽  
M.H.V. Fernandes ◽  
P.M. Vilarinho
Keyword(s):  
Mechanical Properties ◽  
Bioactive Glass ◽  
Average Pore Size ◽  
Physical And Mechanical Properties ◽  
Ceramic Composites ◽  
Porous Polymer ◽  
Bioactive Glasses ◽  
Average Pore ◽  
Thermally Induced ◽  
Ceramic Fillers

Recently, porous polymer-ceramic composites have been developed and represent promising scaffolds to be used as synthetic extracellular matrix in bone tissue engineering since they combine the advantages of these two types of materials. On the other hand bioactive glasses (BG) have been used as ceramic fillers to promote bioactivity and to enhance mechanical properties and osteoblast functions. Among all the requirements, these 3D porous structures should have a controllable average pore size larger than 100 μm as well as good pore interconnectivity to allow vascularization and tissue ingrowth. The goal of this study is to investigate the effect of the addition of a bioactive glass on the porous structure development of the scaffolds prepared by thermally induced phase-separation and also to test the bioactivity of these composite scaffolds. Poly (L-lactic) acid (PLLA) was chosen as the polymer matrix because of its well-known biocompatibility and adjustable physical and mechanical properties. Micron-sized (<10 μm) glass from the 3CaO.P2O5-MgO-SiO2 system was produced in our laboratory and used as the bioactive ceramic filler.

scholarly journals Novel Bioactive Glass-Modified Hybrid Composite Resin: Mechanical Properties, Biocompatibility, and Antibacterial and Remineralizing Activity

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiao Han ◽  
Yan Chen ◽  
Qian Jiang ◽  
Xin Liu ◽  
Yaming Chen
Keyword(s):  
Mechanical Properties ◽  
Bioactive Glass ◽  
Hybrid Composite ◽  
Composite Resin ◽  
Ph Value ◽  
Biological Properties ◽  
Composite Resins ◽  
Mass Fractions ◽  
Good Biocompatibility ◽  
Insight Into

Secondary caries seriously limits the lifetime of composite resin. However, integrating all desirable properties (i.e., mechanical, antibacterial, bioactivity, and biocompatibility) into one composite resin is still challenging. Herein, a novel bioactive glass (BAG)-modified hybrid composite resin has been successfully developed to simultaneously achieve excellent mechanical properties, good biocompatibility, and antibacterial and remineralizing capabilities. When the mass fractions of BAG particles were added from 8 to 23 wt %, the original mechanical properties of the composite resin, including flexural strength and compressive strength, were not obviously affected without compromising the degree of conversion. Although the BAG incorporation of mass fractions of 16 wt % to 23 wt % in composite resins reduced cell viability, the viability could be recovered to normal by adjusting the pH value. Moreover, the BAG-modified composite resins that were obtained showed good antibacterial effects against Streptococcus mutans and enhanced remineralizing activity on demineralized dentin surfaces with increasing incorporation of BAG particles. The possible mechanisms for antibacterial and remineralizing activity might be closely related to the release of bioactive ions (Ca2+, Si4+), suggesting that its antibacterial and biological properties can be controlled by modulating the amounts of bioactive ions. The capability to balance the mechanical properties, cytotoxicity, antibacterial activity, and bioactivity makes the BAG-modified composite resin a promising prospect for clinical application. Our findings provide insight into better design and intelligent fabrication of bioactive composite resins.

scholarly journals Stimulatory Effects of Boron Containing Bioactive Glass on Osteogenesis and Angiogenesis of Polycaprolactone: In Vitro Study

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Lunguo Xia ◽  
Wudi Ma ◽  
Yuning Zhou ◽  
Zhipeng Gui ◽  
Aihua Yao ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Bone Regeneration ◽  
Ph Value ◽  
Umbilical Vein ◽  
In Vitro Study ◽  
Angiogenic Factor ◽  
Potential Applications ◽  
Biological Performance ◽  
Umbilical Vein Endothelial Cells

Polycaprolactone (PCL) has attracted great attention for bone regeneration attributed to its cost-efficiency, high toughness, and good processability. However, the relatively low elastic modulus, hydrophobic nature, and insufficient bioactivity of pure PCL limited its wider application for bone regeneration. In the present study, the effects of the addition of boron containing bioactive glass (B-BG) materials on the mechanical properties and biological performance of PCL polymer were investigated with different B-BG contents (0, 10, 20, 30, and 40 wt.%), in order to evaluate the potential applications of B-BG/PCL composites for bone regeneration. The results showed that the B-BG/PCL composites possess better tensile strength, human neutral pH value, and fast degradation as compared to pure PCL polymers. Moreover, the incorporation of B-BG could enhance proliferation, osteogenic differentiation, and angiogenic factor expression for rat bone marrow stromal cells (rBMSCs) as compared to pure PCL polymers. Importantly, the B-BG also promoted the angiogenic differentiation for human umbilical vein endothelial cells (HUVECs). These enhanced effects had a concentration dependence of B-BG content, while 30 wt.% B-BG/PCL composites achieved the greatest stimulatory effect. Therefore the 30 wt.% B-BG/PCL composites have potential applications in bone reconstruction fields.

scholarly journals Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2661
Author(s):  
Ana Mora-Boza ◽  
Elena López-Ruiz ◽  
María Luisa López-Donaire ◽  
Gema Jiménez ◽  
María Rosa Aguilar ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Polymer Network ◽  
Physicochemical Characterization ◽  
Human Mesenchymal Stem Cells ◽  
Ionic Crosslinking ◽  
Crosslinking Process ◽  
Potential Applications ◽  
Biological Performance

In the present study, semi- and interpenetrated polymer network (IPN) systems based on hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker, glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized and evaluated as potential supports for tissue engineering. Semi- and IPN systems showed significant differences between them regarding composition, morphology, and mechanical properties after physicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retention and mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPN membranes. The biological performance was evaluated on primary human mesenchymal stem cells (hMSCs) and the systems revealed no cytotoxic effect. The excellent biocompatibility of the systems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces. Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN, which suggested that these polymeric membranes could be proposed as an effective promoter system of tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranes can provide a stable and amenable environment for hMSCs support and growth with potential applications in the biomedical field.

scholarly journals Incorporation of Bioactive Glasses Containing Mg, Sr, and Zn in Electrospun PCL Fibers by Using Benign Solvents

2020 ◽  
Vol 10 (16) ◽  
pp. 5530 ◽  
Author(s):  
Rachele Sergi ◽  
Valeria Cannillo ◽  
Aldo R. Boccaccini ◽  
Liliana Liverani
Keyword(s):  
Cell Adhesion ◽  
Bioactive Glass ◽  
Biomedical Application ◽  
Electrospun Fiber ◽  
Glass Composite ◽  
Bioactive Glasses ◽  
Electrospinning Technique ◽  
Fiber Mats ◽  
Cell Adhesion And Proliferation

Poly(ε-caprolactone) (PCL) and PCL/bioactive glass composite fiber mats were produced by electrospinning technique. To improve cell adhesion and proliferation (i) 45S5, (ii) a bioactive glass containing strontium and magnesium oxides, and (iii) a bioactive glass containing zinc oxide were separately added to the starting PCL solution before electrospinning. A good incorporation of bioactive glass particles in PCL electrospun mats was confirmed by SEM and FTIR analyses. Bioactivity was evaluated by immersion of PCL mats and PCL/bioactive glass electrospun fiber mats in simulated body fluid (SBF). Bone murine stromal cells (ST-2) were employed in WST-8 assay to assess cell viability, cell morphology, and proliferation. The results showed that the presence of bioactive glass particles in the fibers enhances cell adhesion and proliferation compared to neat PCL mats. Furthermore, PCL/bioactive glass electrospun mats showed higher wound-healing rate (measured as cell migration rate) in vitro compared to neat PCL electrospun mats. Therefore, the characteristics of the PCL matrix combined with biological properties of bioactive glasses make PCL/bioactive glass composite ideal candidate for biomedical application.

Preparation and characterization of highly porous ceramic-based nanocomposite scaffolds with improved mechanical properties using the liquid phase-assisted sintering method

2018 ◽  
Vol 233 (9) ◽  
pp. 1854-1865
Author(s):  
Mansure Kazemi ◽  
Bahareh Nazari ◽  
Jafar Ai ◽  
Nasrin Lotfibakhshaiesh ◽  
Ali Samadikuchaksaraei ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Culture ◽  
Bioactive Glass ◽  
Tricalcium Phosphate ◽  
Porous Ceramic ◽  
Simulated Body Fluid Solution ◽  
Biological Properties ◽  
Glass Content ◽  
Composite Scaffolds ◽  
Highly Porous

Recent advances in the field of biomaterials have led to the development of ceramic–matrix nanocomposites with enhanced mechanical properties, which is essential for hard tissue scaffolds. In this study, the improvement in mechanical and biological properties of β-tricalcium phosphate reinforced with 45S5 bioactive glass under different sintering conditions was studied. In order to improve the thermal stability and biological responses, β-tricalcium phosphate was doped with 5 mol% strontium ions. Highly porous nanocomposites, with different weight ratios of Sr-tricalcium phosphate/bioactive glass (75/25, 50/50, 25/75), were fabricated through the foam replication method by sintering samples under various thermal conditions (1200–1250 ℃/0–1 h). The effects of bioactive glass content and sintering parameters on microstructure and mechanical behaviors of the nanocomposites were assessed. The obtained results showed that increasing 45S5 bioactive glass content, sintering temperature, and dwelling time gradually improved the mechanical properties of final products which were ascribed to the improved ceramic densification. The composites with the optimal compressive strength were selected to apply in further characterization and cell culture experiments. The selected scaffolds showed excellent bioactivity since a continuous layer of minerals covered the entire surface of composites after immersion in simulated body fluid solution for two weeks. Moreover, the cell culture studies demonstrated that the composite scaffolds could well support the attachment and proliferation of MG-63 osteoblast-like cells. This investigation clearly concluded that the appropriate incorporation of 45S5 bioactive glass into the β-tricalcium phosphate matrix can effectively promote the mechanical behavior, bioactivity, and biocompatibility of the resultant composite scaffolds.

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