scholarly journals Effects of Viscosities and Solution Composition on Core-Sheath Electrospun Polycaprolactone(PCL) Nanoporous Microtubes

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3650
Author(s):  
Yan Chen ◽  
George Z. Tan ◽  
Yingge Zhou
Keyword(s):  
Tissue Engineering ◽  
Pore Size ◽  
Polyethylene Oxide ◽  
Pore Formation ◽  
High Viscosity ◽  
Solution Viscosity ◽  
Solution Composition ◽  
The Past ◽  
Capillary Size ◽  
Arteries And Veins

Vascularization for tissue engineering applications has been challenging over the past decades. Numerous efforts have been made to fabricate artificial arteries and veins, while few focused on capillary vascularization. In this paper, core-sheath electrospinning was adopted to fabricate nanoporous microtubes that mimic the native capillaries. The results showed that both solution viscosity and polyethylene oxide (PEO) ratio in polycaprolactone (PCL) sheath solution had significant effects on microtube diameter. Adding PEO into PCL sheath solution is also beneficial to surface pore formation, although the effects of further increasing PEO showed mixed results in different viscosity groups. Our study showed that the high viscosity group with a PCL/PEO ratio of 3:1 resulted in the highest average microtube diameter (2.14 µm) and pore size (250 nm), which mimics the native human capillary size of 1–10 µm. Therefore, our microtubes show high potential in tissue vascularization of engineered scaffolds.

scholarly journals 3D-Bioprinting Strategies Based on In Situ Bone-Healing Mechanism for Vascularized Bone Tissue Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 287
Author(s):  
Ye Lin Park ◽  
Kiwon Park ◽  
Jae Min Cha
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Critical Size ◽  
Current Knowledge ◽  
3D Bioprinting ◽  
The Past ◽  
Regeneration Processes

Over the past decades, a number of bone tissue engineering (BTE) approaches have been developed to address substantial challenges in the management of critical size bone defects. Although the majority of BTE strategies developed in the laboratory have been limited due to lack of clinical relevance in translation, primary prerequisites for the construction of vascularized functional bone grafts have gained confidence owing to the accumulated knowledge of the osteogenic, osteoinductive, and osteoconductive properties of mesenchymal stem cells and bone-relevant biomaterials that reflect bone-healing mechanisms. In this review, we summarize the current knowledge of bone-healing mechanisms focusing on the details that should be embodied in the development of vascularized BTE, and discuss promising strategies based on 3D-bioprinting technologies that efficiently coalesce the abovementioned main features in bone-healing systems, which comprehensively interact during the bone regeneration processes.

scholarly journals Oxygen Permeability of Silk Fibroin Hydrogels and Their Use as Materials for Contact Lenses: A Purposeful Analysis

Gels ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 58
Author(s):  
Traian V. Chirila
Keyword(s):  
Tissue Engineering ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Oxygen Permeability ◽  
Contact Lenses ◽  
The Past ◽  
Fibrous Protein ◽  
Silk Moth ◽  
Bombyx Mori Silk

Fibroin is a fibrous protein that can be conveniently isolated from the silk cocoons produced by the larvae of Bombyx mori silk moth. In its form as a hydrogel, Bombyx mori silk fibroin (BMSF) has been employed in a variety of biomedical applications. When used as substrates for biomaterial-cells constructs in tissue engineering, the oxygen transport characteristics of the BMSF membranes have proved so far to be adequate. However, over the past three decades the BMSF hydrogels have been proposed episodically as materials for the manufacture of contact lenses, an application that depends on substantially elevated oxygen permeability. This review will show that the literature published on the oxygen permeability of BMSF is both limited and controversial. Additionally, there is no evidence that contact lenses made from BMSF have ever reached commercialization. The existing literature is discussed critically, leading to the conclusion that BMSF hydrogels are unsuitable as materials for contact lenses, while also attempting to explain the scarcity of data regarding the oxygen permeability of BMSF. To the author’s knowledge, this review covers all publications related to the topic.

Smurf1-targeting miR-19b-3p-modified BMSCs combined PLLA composite scaffold to enhance osteogenic activity and treat critical-sized bone defects

2020 ◽  
Vol 8 (21) ◽  
pp. 6069-6081
Author(s):  
Ao Xiong ◽  
Yijun He ◽  
Liang Gao ◽  
Guoqing Li ◽  
Jian Weng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Composite Scaffold ◽  
Biological Materials ◽  
Bone Defects ◽  
Engineering Technology ◽  
Osteogenic Activity ◽  
The Past ◽  
Seed Cells ◽  
Tissue Engineering Technology

Over the past few years, tissue-engineering technology provided a new direction for bone defects therapy, which involved developing applicable biological materials composite with seed cells to repair bone defects tissue.

Preparation of Nano-Fibrous Poly(L-lactic acid) Scaffold with Hierarchical Pores

2011 ◽  
Vol 418-420 ◽  
pp. 303-306
Author(s):  
Xue Jun Wang ◽  
Tao Lou ◽  
Guo Jun Song
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Pore Size ◽  
Composite Scaffold ◽  
Compressive Modulus ◽  
High Porosity ◽  
Nano Composite ◽  
Hierarchical Pores ◽  
Fiber Size ◽  
Hierarchical Pore

In this study, a nano-fibrous PLLA scaffold with hierarchical pore was sucessfully fabricated using combined TIPS and particle leaching method.The scaffold had a nano-fibrous PLLA matrix (fiber size 100-800 nm), an interconnective hierarchical pores (1.0- 425 μm), high porosity (>96%). The compressive modulus of scaffold with different pore size was between 0.16 MPa to 0.2 Mpa and it decreased with the increased salt size embedded in. The new nano composite scaffold is potentially a very promising scaffold for tissue engineering.

The effect of fiber size and pore size on cell proliferation and infiltration in PLLA scaffolds on bone tissue engineering

2016 ◽  
Vol 30 (10) ◽  
pp. 1545-1551 ◽  
Author(s):  
Xuejun Wang ◽  
Tao Lou ◽  
Wenhua Zhao ◽  
Guojun Song ◽  
Chunyao Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Bone Tissue Engineering ◽  
Pore Size ◽  
Bone Tissue ◽  
Fiber Size

scholarly journals Ca(2+) Oscillations and Its Transporters in Mesenchymal Stem Cells

2010 ◽  
pp. 323-329 ◽  
Author(s):  
B Ye
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Replacement Therapy ◽  
Cell Types ◽  
Cell Replacement Therapy ◽  
Cell Replacement ◽  
Cell Functions ◽  
The Past ◽  
Intracellular Ca

Intracellular free Ca(2+) is one of important biological signals regulating a number of cell functions. It has been discussed widely and extensively in several cell types during the past two decades. Attention has been paid to the Ca2+ transportation in mesenchymal stem cells in recent years as mesenchymal stem cells have gained considerable interest due to their potential for cell replacement therapy and tissue engineering. In this paper, roles of intracellular Ca(2+) oscillations and its transporters in mesenchymal stem cells have been reviewed.

Development of Scaffold Building Units and Assembly for Tissue Engineering Using Fused Deposition Modelling

2009 ◽  
Vol 83-86 ◽  
pp. 269-274 ◽  
Author(s):  
Syed H. Masood ◽  
Kadhim Alamara
Keyword(s):  
Tissue Engineering ◽  
Pore Size ◽  
Fused Deposition Modeling ◽  
Porous Scaffold ◽  
Cell Structure ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Fused Deposition Modelling ◽  
Fused Deposition ◽  
Unit Cells

In tissue engineering (TE), a porous scaffold structure of biodegradable material is required as a template to guide the proliferation, growth and development of cells appropriately in three dimensions. The scaffold must meet design requirements of appropriate porosity, pore size and interconnected structure to allow cell proliferation and adhesion. This paper presents a methodology for design and manufacture of TE scaffolds with varying porosity by employing open structure building units and Fused Deposition Modeling (FDM) rapid prototyping technique. A computer modeling approach for constructing and assembly of three-dimensional unit cell structure is presented to provide a solution of scaffolds design that can potentially meet the diverse requirements of TE applications. A parametric set of open polyhedral unit cells is used to assist the user in designing the required micro-architecture of the scaffold with required porosity and pore size and then the Boolean operation is used to create the scaffold of a given CAD model from the designed microstructure. The procedure is verified by fabrication of physical scaffolds using the commercial FDM system.

Solution viscosity regulates chondrocyte proliferation and phenotype during 3D culture

2019 ◽  
Vol 7 (48) ◽  
pp. 7713-7722 ◽  
Author(s):  
Kyubae Lee ◽  
Yazhou Chen ◽  
Xiaomeng Li ◽  
Yongtao Wang ◽  
Naoki Kawazoe ◽  
...  
Keyword(s):  
3D Culture ◽  
High Viscosity ◽  
Solution Viscosity ◽  
Cell Phenotype ◽  
Gelatin Solution ◽  
Chondrocyte Proliferation ◽  
Low Viscosity ◽  
System Solution ◽  
Hydrogel System

Chondrocytes are cultured in a 3D biphasic gelatin solution/hydrogel system. Solution viscosity affects chondrocyte functions. High viscosity is more beneficial for cell phenotype maintenance, while low viscosity is more beneficial for proliferation.

Design and additive manufacturing of porous titanium scaffolds for optimum cell viability in bone tissue engineering

2020 ◽  
pp. 095440542093756
Author(s):  
Bingbing Li ◽  
Bani Davod Hesar ◽  
Yiwen Zhao ◽  
Li Ding
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Bone Tissue Engineering ◽  
Pore Size ◽  
Bone Tissue ◽  
Structural Strength ◽  
Three Dimensional ◽  
Porous Titanium ◽  
Nih3t3 Cells ◽  
Pore Sizes

Pore size, external shape, and internal complexity of additively manufactured porous titanium scaffolds are three primary determinants of cell viability and structural strength of scaffolds in bone tissue engineering. To obtain an optimal design with the combination of all three determinants, four scaffolds each with a unique topology (external geometry and internal structure) were designed and varied the pore sizes of each scaffold 3 times. For each topology, scaffolds with pore sizes of 300, 400, and 500 µm were designed. All designed scaffolds were additively manufactured in material Ti6Al4V by the direct metal laser melting machine. Compression test was conducted on the scaffolds to assure meeting minimum compressive strength of human bone. The effects of pore size and topology on the cell viability of the scaffolds were analyzed. The 12 scaffolds were ultrasonically cleaned and seeded with NIH3T3 cells. Each scaffold was seeded with 1 million cells. After 32 days of culturing, the cells were fixed for their three-dimensional architecture preservation and to obtain scanning electron microscope images.

scholarly journals Effects of Encapsulated Cells on the Physical–Mechanical Properties and Microstructure of Gelatin Methacrylate Hydrogels

2019 ◽  
Vol 20 (20) ◽  
pp. 5061 ◽  
Author(s):  
Srikumar Krishnamoorthy ◽  
Behnam Noorani ◽  
Changxue Xu
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Viability ◽  
Physical Properties ◽  
Pore Size ◽  
Cell Density ◽  
Tensile Modulus ◽  
Encapsulated Cells ◽  
3T3 Fibroblasts ◽  
Cell Densities

Gelatin methacrylate (GelMA) has been gaining popularity in recent years as a photo-crosslinkable biomaterial widely used in a variety of bioprinting and tissue engineering applications. Several studies have established the effects of process-based and material-based parameters on the physical–mechanical properties and microstructure of GelMA hydrogels. However, the effect of encapsulated cells on the physical–mechanical properties and microstructure of GelMA hydrogels has not been fully understood. In this study, 3T3 fibroblasts were encapsulated at different cell densities within the GelMA hydrogels and incubated over 96 h. The effects of encapsulated cells were investigated in terms of mechanical properties (tensile modulus and strength), physical properties (swelling and degradation), and microstructure (pore size). Cell viability was also evaluated to confirm that most cells were alive during the incubation. It was found that with an increase in cell density, the mechanical properties decreased, while the degradation and the pore size increased.

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