Application of Butterfly Pea Flower Extract in Mask Development

(1) Background: Clitoria ternatea (butterfly pea), a plant species belonging to the Leguminosae (Fabaceae) family, is useful for medical treatments and has been used in folk medicines and to cure different diseases. The antioxidation ability of the total phenolic compounds of butterfly pea is useful for preserving flavor, and colour and for preventing vitamin destruction in processed foods. In this study, a butterfly pea flower fermentation solution was added to cosmetics as a whiting ingredient. (2) Methods: After the phenolics, flavonoids and ascorbic acid content of the butterfly pea flower extraction had been determined, lactic acid bacteria fermented the extraction. The whitening and moisturizing effect was assayed by SSC3 and NF333 analyzers. (3) Results: This study demonstrated that the butterfly pea flower fermentation solution has free radical scavenging ability, a reducing power in high concentrations, a moisturizing effect, and a whiting effect. (4) Conclusions: The results showed that the butterfly pea flower fermentation solution not only inhibits redness, itching, allergies, and irritation to the skin, but also has antioxidation properties and promotes moisture retention and whitening effects, and the results increase as the concentration increases. Therefore, butterfly bean flowers may be suitable as a raw material for natural beauty care products.

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Assessment of flavonoids and phenolic compound accumulation in invasive Solidago canadensis L. in Slovakia

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1378 ◽

2020 ◽

Vol 14 ◽

pp. 587-594

Author(s):

Olga Shelepova ◽

Yulia Vinogradova ◽

Olena Vergun ◽

Olga Grygorieva ◽

Ján Brindza

Keyword(s):

Phenolic Compounds ◽

Therapeutic Potential ◽

Radical Scavenging ◽

Reducing Power ◽

Total Flavonoid ◽

Raw Material ◽

Herbal Remedies ◽

Total Phenolic ◽

Solidago Canadensis ◽

Dpph Method

Solidago canadensis L. was introduced to Europe as an ornamental plant from North America in 1645 and began to spread during the XIX-XX centuries. Nowadays the species is considered the most aggressive invasive species. On the other hand, S. canadensis is considered to be a medicinal plant. The raw material known as Herba Solidaginis includes herbs of S. canadensis, S. gigantea, and S. virgaurea. These species are known for their diuretic, anti-inflammatory, antimicrobial, antioxidant, antispasmodic properties. The purpose of our study was to analyze the chemical compounds and some biological properties of S. canadensis, growing in Slovakia, to evaluate its therapeutic potential. The total phenolic content (TPC) of the extracts from aerial parts of S. canadensis was determined by the Folin-Ciocalteu method. The evaluation of total flavonoid content (TFC) was performed by using a spectrophotometric method. The flavonoids content was expressed as rutin equivalents (mg REs) per g DW vegetal product. The phytochemical profile of S. canadensis extracts was assessed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The radical scavenging activity of samples was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Reducing power of extracts was determined by the phosphomolybdenum method. Total phenolic contents (TPC) and total flavonoid contents (TFC) of the extracts varied from 204.19 to 293.43 mg GAE.g-1 DW, and 64.99 – 175.25 g QE.g-1 DW, respectively; the best results were obtained for ethanol extract. Some phenolic compounds were identified by HPLC with significant amounts of rutin (211.20 µg.mL-1), quercetin (122.08 µg.mL-1), quercitrin (102.50 µg.mL-1) and chlorogenic acid (147.00 µg.mL-1). The DPPH values in the inflorescences were higher than in the leaves: the antioxidant activity of leaf extracts was in the range from 5.34 to 17.16 mg TE.g-1, for inflorescences, this parameter ranged from 6.09 to 19.87 mg TE.g-1. The high total phenolic compounds and flavonoids can be used as a valuable source of phytochemicals in herbal remedies. Our study of S. canadensis, growing in Slovakia, shows the promising potential that can be evaluated as an effective antioxidant and antimicrobial agent in herbal medicines.

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Biotransformation of soy whey into a novel functional beverage by Cordyceps militaris SN-18

Food Production, Processing and Nutrition ◽

10.1186/s43014-021-00054-0 ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Yiqiang Dai ◽

Jianzhong Zhou ◽

Lixia Wang ◽

Mingsheng Dong ◽

Xiudong Xia

Keyword(s):

Dna Damage ◽

High Performance ◽

Radical Scavenging ◽

Reducing Power ◽

Essential Amino Acids ◽

Cordyceps Militaris ◽

Total Phenolic ◽

Functional Beverages ◽

Radical Scavenging Ability ◽

Phenolic And Flavonoid

Abstract Soy whey, a liquid nutritional by-product of soybean manufacture, is rich in proteins, oligosaccharides and isoflavones. Soy whey can be used to produce functional beverages, instead of discarding it as a waste. In this study, unfermented soy whey (USW) and Cordyceps militaris SN-18-fermented soy whey (FSW) were investigated and compared for their physicochemical and functional properties by high performance liquid chromatography (HPLC) and DNA damage assay. Results show that C. militaris SN-18 fermentation could increase the contents of essential amino acids, total phenolic and flavonoid and isoflavone aglycones and eliminate the oligosaccharides in soy whey. Furthermore, C. militaris SN-18 could significantly enhance the ABTS radical scavenging ability, reducing power and ferric reducing power of soy whey, and its fermented products could prominently attenuate Fenton reaction-induced DNA damage. These findings indicate that soy whey can potentially be converted into a novel soy functional beverage by C. militaris SN-18 fermentation. Graphical abstract

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Biochemical, Phytochemical and Antioxidant Analysis of Leaves and Seed of Fenugreek Plant

FRONTIERS IN CHEMICAL SCIENCES ◽

10.52700/fcs.v1i2.8 ◽

2020 ◽

Vol 1 (2) ◽

pp. 9-18

Author(s):

Haq Nawaz ◽

Hina Andaleeb ◽

Sobia Aziz ◽

Saba Rehman

Keyword(s):

Ascorbic Acid ◽

Acid Content ◽

Reducing Sugars ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Ascorbic Acid Content ◽

Dry Weight ◽

Water Soluble ◽

Total Phenolic

In the present study leaves and seeds of two different varieties of Fenugreek plant viz. Trigonella foenum gracium and Trigonella corniculata, collected from Bahawalpur, Pakistan were subjected to the biochemical, phytochemical and antioxidant analysis. The content of total sugars, non reducing sugars and reducing sugars in selected parts of these varities were found to be 5.007±0.101 to 8.133±0.223, 4.255±0.150 to 7.099±0.316 and 0.751±0.064 to 1.410±0.071 g/100g of dry weight respectively. Water soluble, salt soluble and total salt soluble protein content range from 1.172±0.001 to 6.015±0.132, 1.517±0.038 to 1.174±0.006 and 0.013±0.001 to 0.018±0.001 g/100 g of dry weight respectively and the total free amino acids ranged from 1.198±0.014 to 4.554±0.006 g/100 g of dry weight. The range of tannins and saponin contents in analyzed parts of these varities was found to be 0.057±0.009 to 0.117±0.025 and 0.083±0.012 to 0.0151±0.045 g /100g of dry weight respectively. Similarly, flavonoids and hydrogen cyanide content ranged from 0.021±0.000 to 0.055±0.004 and 0.0234±0.008 to 0.307±0.009 g/100g of dry weight respectively; while ascorbic acid content was found to be in the range of 0.068±0.08 to 0.267±0.031 g/100g of dry weight. Total phenolic acid content of the selected parts of these varities were estimated on the basis of difference in dipole moment and its range was observed from 0.0539±0.024 to 0.856±0.064 g/100g of dry weight. The total antioxidants analysis showed that ascorbic acid and trolox contents (mg Eqv./100g of extract) were found to be lower in Trigonella corniculata seeds (0.066±0.001 and 0.047±0.001 mg Eqv./100g respectively) and higher in Trigonella corniculata leaves (0.069±0.001 and 0.050±0.001 mg Eqv./100g respectively).The DPPH radical scavenging activity and reducing power were found to be in the range of 31.736±2.430 to 39.590±2.432 and 0.974±0.231 to 2.301±0.068 respectively. The different parts of studied varities of fenugreek plant, having higher values of %DPPH inhibition and reducing power showed least count of IC50 and vice versa.

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Bioactive compounds from Syzygium malaccense leaves: optimization of the extraction process, biological and chemical characterization

Acta Scientiarum Technology ◽

10.4025/actascitechnol.v42i1.46773 ◽

2020 ◽

Vol 42 ◽

pp. e46773 ◽

Cited By ~ 2

Author(s):

Aline Savi ◽

Matheus Augusto Calegari ◽

Gabrielle Cristina Calegari ◽

Vidiany Aparecida Queiroz Santos ◽

Denise Wermuth ◽

...

Keyword(s):

Bioactive Compounds ◽

Radical Scavenging ◽

Reducing Power ◽

Chemical Characterization ◽

Ethanolic Extract ◽

Extraction Process ◽

Total Phenolic ◽

Radical Scavenging Ability

Syzygium malaccense belongs to the family Myrtaceae and it is popularly recognised as ‘jambo’ in Brazil. It is poorly studied regarding the bioactive compounds of fruits and leaves. This study aimed at optimizing the extraction and characterization of bioactives compounds from S. malaccense leaves. Extraction was optimized using a 23 full factorial design to evaluate the effect of ethanol concentration, time and temperature on polyphenols and antioxidants contents. Analyses of phenolic compounds content (Folin-Ciocalteau and HPLC-DAD), in vitro antioxidant capacities (DPPH, ABTS and FRAP), antimicrobial activity against bacteria and yeasts of ethanolic extract was carried out in optimal conditions of extraction. The optimized extraction condition was 45 min., 80°C and 40:60 (v v-1) ethanol:water. The extract showed high total phenolic content (0.074 mg GAE kg-1), DPPH (0.666 μmol TE kg-1) and ABTS (0.853 μmol TE kg-1) free radical scavenging ability and appreciable ferric reducing power (1.267 μmol Fe2+ kg-1). HPLC methodology allowed the identification of four bioactive compounds: gallic acid (0.00036 mg kg-1), catechin (0.00021 mg kg-1), rutin (0.00027 mg kg-1) and quercetin (0.00003 mg kg-1). The extract showed inhibitory activity against S. aureus (1 µg L-1); S. bongori (8 µg L-1), C. albicans and C. tropicalis (2 µg L-1). It also showed microbicidal potential against S. aureus; C. albicans and C. tropicalis.

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Hepatoprotective studies on methanolic extract fractions of Lindernia ciliata and development of qualitative analytical profile for the bioactive extract

Clinical Phytoscience ◽

10.1186/s40816-019-0123-1 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Praneetha Pallerla ◽

Narsimha Reddy Yellu ◽

Ravi Kumar Bobbala

Keyword(s):

Methanolic Extract ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Liver Homogenate ◽

Reducing Power ◽

Hepatoprotective Activity ◽

Total Phenolic ◽

Effective Fraction

Abstract Background The objective of the study is to evaluate the hepatoprotective activity of methanolic extract fractions of Lindernia ciliata (LC) and development of qualitative analytical profile of the bioactive fraction using HPLC fingerprinting analysis. All the fractions of methanolic extract of Lindernia ciliata (LCME) are assessed for their total phenolic, flavonoid contents and in vitro antioxidant properties by using DPPH, superoxide, nitric oxide, hydroxyl radical scavenging activities and reducing power assay. Acute toxicity study was conducted for all the fractions and the two test doses 50 and 100 mg/kg were selected for the hepatoprotective study. Liver damage was induced in different groups of rats by administering 3 g/kg.b.w.p.o. paracetamol and the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters and histology of liver of rats. The effective fraction was evaluated for its antihepatotoxic activity against D-Galactosamine (400 mg/kg b.w. i.p.) and in vivo antioxidant parameters viz., Glutathione (GSH), Melondialdehyde (MDA) and Catalase (CAT) levels are estimated using liver homogenate. Results Among all the fractions, butanone fraction of LCME, (BNF-LCME) has shown better hepatoprotective activity and hence it is selected to evaluate the antihepatotoxicity against D-GaIN. The activity of BNF-LCME is well supported in in vitro and in vivo antioxidant studies and may be attributed to flavonoidal, phenolic compounds present in the fraction. Hence, BNF-LCME was subjected to the development of qualitative analytical profile using HPLC finger printing analysis. Conclusions All the fractions of LCME exhibited significant hepatoprotective activity and BNF-LCME (50 mg/kg) was identified as the most effective fraction.

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Preparation of Antioxidant and Antibacterial Chitosan Film from Periplaneta americana

Insects ◽

10.3390/insects12010053 ◽

2021 ◽

Vol 12 (1) ◽

pp. 53

Author(s):

Sicong Chen ◽

Xunfan Wei ◽

Zhuoxiao Sui ◽

Mengyuan Guo ◽

Jin Geng ◽

...

Keyword(s):

Food Packaging ◽

Periplaneta Americana ◽

Uv Light ◽

Radical Scavenging ◽

Chitosan Film ◽

American Cockroach ◽

Raw Material ◽

Potential Resource ◽

Radical Scavenging Ability ◽

Dpph Radical Scavenging

Among different insects, the American cockroach (Periplaneta americana) has been bred in industrial scale successfully as a potential resource of protein, lipid, and antibacterial peptide. However, the application of its chitosan has not been studied widely, which has hindered the sufficient utilization of P. americana. In this paper, the chitosan from P. americana was separated, characterized, and processed into film (PaCSF) to examine its potential of being applied in food packaging. As the results of different characterizations showed, PaCSF was similar to shrimp chitosan film (SCSF). However, concerning the performances relating to food packaging, the two chitosan films were different. PaCSF contained more water (42.82%) than SCSF did, resulting in its larger thickness (0.08 mm). PaCSF could resist UV light more effectively than SCSF did. Concerning antioxidant activity, the DPPH radical scavenging ability of PaCSF increased linearly with time passing, reaching 72.46% after 8 h, which was better than that of SCSF. The antibacterial activity assay exhibited that PaCSF resisted the growth of Serratia marcescens and Escherichia coli more effectively than SCSF did. The results implied that P. americana chitosan could be a potential raw material for food packaging, providing a new way to develop P. americana.

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New insights of phenolic compounds from optimized fruit extract of Ficus auriculata

Scientific Reports ◽

10.1038/s41598-021-91913-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

M. Shahinuzzaman ◽

Parul Akhtar ◽

N. Amin ◽

Yunus Ahmed ◽

Farah Hannan Anuar ◽

...

Keyword(s):

Antioxidant Activity ◽

Bioactive Compounds ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Fruit Extract ◽

Optimum Extraction

AbstractIn this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

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Potential Application of Cornelian Cherry Extract on Broiler Chickens: Growth, Expression of Antioxidant Biomarker and Glucose Transport Genes, and Oxidative Stability of Frozen Meat

Animals ◽

10.3390/ani11041038 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1038

Author(s):

Doaa Ibrahim ◽

Amira Moustafa ◽

Aya Sh. Metwally ◽

Mohamed A. Nassan ◽

Karima Abdallah ◽

...

Keyword(s):

Oxidative Stability ◽

Broiler Chickens ◽

Radical Scavenging ◽

Reducing Power ◽

Poultry Feed ◽

Total Phenolic ◽

Control Group ◽

Breast Meat ◽

Antioxidant Genes ◽

Cornelian Cherry

The use of natural plant extracts in poultry feed could improve their productivity as well as the oxidative stability of stored derived meat. The roles of cornelian cherry extract (CCE) in growth, cecal microbes, and meat antioxidative markers of broiler chickens were evaluated. A total of 500 Ross 308 broiler chicks were fed diets supplemented with CCE (0, 50, 100, 200, 400 mg/kg of diet) for 38 days. The highest levels of weight gain and feed utilization were observed in a group fed 200 mg/kg of CCE. Maximum upregulation of glucose transporters—1 and 2 and sodium-dependent glucose transporter genes—were found in the group fed 200 mg/kg of CCE. Lactobacilli and Bifidobacterium colonization increased as the CCE levels increased. The greatest upregulation of antioxidant genes (glutathione peroxidase, catalase, and superoxide dismutase) in breast meat was observed in groups fed CCE (200 and 400 mg/kg). Dietary CCE significantly delayed the lipid oxidation of breast meat compared with that of the control group. The total phenolic content, 2,2-Diphenyl-1-Picrihydrzyl (DPPH) radical scavenging activity and reducing power in meat improved with higher levels of CCE. Dietary CCE improved the growth, performance of broilers, and meat antioxidant stability after 90 days of storage.

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Triterpene Acid and Phenolics from Ancient Apples of Friuli Venezia Giulia as Nutraceutical Ingredients: LC-MS Study and In Vitro Activities

Molecules ◽

10.3390/molecules24061109 ◽

2019 ◽

Vol 24 (6) ◽

pp. 1109 ◽

Cited By ~ 10

Author(s):

Stefania Sut ◽

Gokhan Zengin ◽

Filippo Maggi ◽

Mario Malagoli ◽

Stefano Dall’Acqua

Keyword(s):

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Inhibitory Effects ◽

Triterpene Acid ◽

Beneficial Effects ◽

Triterpene Acids ◽

Phenolic Constituents ◽

Friuli Venezia Giulia

Triterpene acid and phenolic constituents from nine ancient varieties of apple (Malus domestica) fruits cultivated in Fanna, Friuli Venezia Giulia region, northeast Italy, were analyzed and compared with four commercial apples (‘Golden Delicious’, ‘Red Delicious’, ‘Granny Smith’ and ‘Royal Gala’). Total phenolic and flavonoid contents were measured by spectrophotometric assays. The quali-quantitative fingerprint of secondary metabolites including triterpene acid was obtained by LC-DAD-(ESI)-MS and LC-(APCI)-MS, respectively. Based on the two LC-MS datasets, multivariate analysis was used to compare the composition of ancient fruit varieties with those of four commercial apples. Significant differences related mainly to the pattern of triterpene acids were found. Pomolic, euscaphyc, maslinic and ursolic acids are the most abundant triterpene in ancient varieties pulps and peels, while ursolic and oleanolic acids were prevalent in the commercial fruits. Also, the content of the phenolic compounds phloretin-2-O-xyloglucoside and quercetin-3-O-arabinoside was greater in ancient apple varieties. The antioxidant (radical scavenging, reducing power, metal chelating and phosphomolybdenum assays) and enzyme inhibitory effects (against cholinesterase, tyrosinase, amylase and glucosidase) of the samples were investigated in vitro. Antioxidant assays showed that the peels were more active than pulps. However, all the samples exhibited similar enzyme inhibitory effects. Ancient Friuli Venezia Giulia apple cultivars can be a source of chlorogenic acid and various triterpene acids, which are known for their potential anti-inflammatory activity and beneficial effects on lipid and glucose metabolism. Our results make these ancient varieties suitable for the development of new nutraceutical ingredients.

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Antioxidant Potential in Different Parts and Callus ofGynura procumbensand Different Parts ofGynura bicolor

BioMed Research International ◽

10.1155/2015/147909 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 15

Author(s):

Vijendren Krishnan ◽

Syahida Ahmad ◽

Maziah Mahmood

Keyword(s):

Ascorbic Acid ◽

Acid Content ◽

Phenolic Content ◽

Reducing Power ◽

Ascorbic Acid Content ◽

Total Phenolic ◽

Total Flavonoid Content ◽

Dpph Radical ◽

Flavonoid Content ◽

Different Parts

Plants from Gynura family was used in this study, namely,Gynura procumbensandGynura bicolor.Gynura procumbensis well known for its various medicinal properties such as antihyperglycaemic, antihyperlipidaemic, and antiulcerogenic; meanwhile,G. bicolorremains unexploited. Several nonenzymatic antioxidants methods were utilized to study the antioxidant capacity, which include ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, total flavonoid content, total phenolic content, and ascorbic acid content determination. DPPH assay revealsG. procumbensshoot as the lowest (66.885%) andG. procumbensroot as the highest (93.499%) DPPH radical inhibitor. In FRAP assay, reducing power was not detected inG. procumbensleaf callus (0.000 TEAC mg/g FW) wherebyG. procumbensroot exhibits the highest (1.103 TEAC mg/g FW) ferric reducing power. Total phenolic content and total flavonoid content exhibited similar trend for both the intact plants analysed. In all antioxidant assays,G. procumbenscallus culture exhibits very low antioxidant activity. However,G. procumbensroot exhibited highest phenolic content, flavonoid content, and ascorbic acid content with 4.957 TEAC mg/g FW, 543.529 QEµg/g FW, and 54.723 µg/g FW, respectively. This study reveals thatG. procumbensroot extract is a good source of natural antioxidant.

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