scholarly journals DES Based Efficient Extraction Method for Bioactive Coumarins from Angelica dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav.

Separations ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 5
Author(s):  
Ting Wang ◽  
Qian Li
Keyword(s):  
Choline Chloride ◽  
Radical Scavenging ◽  
Deep Eutectic Solvents ◽  
Extraction Yield ◽  
Molar Ratio ◽  
Angelica Dahurica ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Before And After ◽  
Ultrasonic Assisted

In this study, a simple and environmentally friendly method was developed for the extraction of seven active coumarins from Angelica dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav.(A. dahurica) based on deep eutectic solvents (DESs). Among the 16 kinds of DES based on choline chloride, the DES system with the molar ratio of choline chloride, citric acid, and water as 1:1:2 had the best extraction effect. Ultrasonic-assisted response surface methodology (RSM) was used to investigate the optimal extraction scheme. The results showed that the optimal extraction conditions were a liquid–solid ratio of 10:1 (mL/g), an extraction time of 50 min, an extraction temperature of 59.85 °C, and a moisture content of 49.28%. Under these conditions, the extraction yield reached 1.18%. In addition, scanning electron microscopy (SEM) was used to observe the degree of powder fragmentation before and after extraction with different solvents. The cells of A. dahurica medicinal materials obtained by DES ultrasonic-assisted treatment were the most seriously broken, indicating that DES had the highest efficiency in the treatment of A. dahurica. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) DPPH radical scavenging model was used to evaluate the biological activity of DES extract. The results showed that DES extract had better scavenging ability of DPPH free radical. Therefore, DES is a green solvent suitable for extracting coumarin compounds of A. dahurica, with great potential to replace organic solvents.

scholarly journals Natural Deep Eutectic Solvents for the Extraction of Bioactive Steroidal Saponins from Dioscoreae Nipponicae Rhizoma

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2079
Author(s):  
Gui-Ya Yang ◽  
Jun-Na Song ◽  
Ya-Qing Chang ◽  
Lei Wang ◽  
Yu-Guang Zheng ◽  
...  
Keyword(s):  
Extraction Efficiency ◽  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Molar Ratio ◽  
Macroporous Resin ◽  
Steroidal Saponins ◽  
Solid Ratio ◽  
Natural Deep Eutectic Solvents ◽  
Before And After ◽  
Extraction Parameters

In the present study, a simple and environmentally friendly extraction method based on natural deep eutectic solvents (NADESs) was established to extract four bioactive steroidal saponins from Dioscoreae Nipponicae Rhizoma (DNR). A total of twenty-one types of choline chloride, betaine, and L-proline based NADESs were tailored, and the NADES composed of 1:1 molar ratio of choline chloride and malonic acid showed the best extraction efficiency for the four steroidal saponins compared with other NADESs. Then, the extraction parameters for extraction of steroidal saponins by selected tailor-made NADES were optimized using response surface methodology and the optimal extraction conditions are extraction time, 23.5 min; liquid–solid ratio, 57.5 mL/g; and water content, 54%. The microstructure of the DNR powder before and after ultrasonic extraction by conventional solvents (water and methanol) and the selected NADES were observed using field emission scanning electron microscope. In addition, the four steroidal saponins were recovered from NADESs by D101 macroporous resin with a satisfactory recovery yield between 67.27% and 79.90%. The present research demonstrates that NADESs are a suitable green media for the extraction of the bioactive steroidal saponins from DNR, and have a great potential as possible alternatives to organic solvents for efficiently extracting bioactive compounds from natural products.

scholarly journals Process Optimization of Ultrasonic-Assisted Extraction of Arabinogalactan from Dihydroquercetin Extracted Residues by Response Surface Methodology and Evaluation of Its Antioxidant Activity

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Zaizhi Liu ◽  
Chunhui Ma ◽  
Lei Yang ◽  
Yuangang Zu
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Before And After ◽  
Ultrasonic Assisted ◽  
Optimal Extraction Condition

Ultrasound was used for the extraction of larch arabinogalactan fromLarix gmelinii. The optimal conditions for ultrasound extraction were determined by response surface methodology. Specifically, the Box-Behnken design was employed to evaluate the effects of three independent variables: ultrasound time, temperature, and liquid-solid ratio. The highest arabinogalactan yield (11.18%) was obtained under the optimal extraction condition (extraction temperature 41.5°C, extraction time 24.3 min, and liquid-solid ratio 40 mL/g). In addition, the antioxidant activity of arabinogalactan that was extracted from dihydroquercetin extraction residues exhibited a moderate and concentration-dependent hydroxyl radical-scavenging capacity, ferric-reducing power, and reducing power. The wood material was characterized before and after processing by scanning electron microscopy and Fourier-transform infrared spectroscopy.

scholarly journals Deep Eutectic Solvents Based Ultrasonic Extraction of Polysaccharides from Edible Brown Seaweed Sargassum horneri

2020 ◽  
Vol 8 (6) ◽  
pp. 440
Author(s):  
Jinggui Nie ◽  
Danting Chen ◽  
Yanbin Lu
Keyword(s):  
Choline Chloride ◽  
Brown Seaweed ◽  
Deep Eutectic Solvents ◽  
Significant Inhibition ◽  
Hot Water ◽  
Ultrasonic Extraction ◽  
Sargassum Horneri ◽  
Molar Ratio ◽  
Extraction Temperature

In this work, a method for ultrasonic extraction of polysaccharides from Sargassum horneri using deep eutectic solvents was proposed. The studied deep eutectic solvents were composed of choline chloride, 1,2-propanediol and water. Based on the single-factor experiment results, four experimental factors were systematically evaluated, giving the optimal extraction conditions as follows: molar ratio of choline chloride to 1,2-propanediol of 1:2, water content of 30% (v/v), solid-liquid ratio of 1:30 (g/mL), and the extraction temperature of 70 °C. Fourier transform infrared spectroscopy and X-ray diffraction were utilized to investigate changes in the chemical characteristic of extracted polysaccharides. The results indicated that deep eutectic solvents had stronger protein and calcium carbonate removal ability than that of a conventional hot water extraction method. Moreover, in vitro antioxidant activity tests exhibited that the obtained polysaccharides had significant inhibition effects on DPPH and ABTS radicals. The proposed deep eutectic solvents assisted ultrasonic extraction protocol was considered to be a green, fast and effective protocol for extracting polysaccharides from Sargassum horneri.

Deacidification of Crude Rice Bran Oil (CRBO) to Remove FFA and Preserve γ-Oryzanol Using Deep Eutectic Solvents (DES)

2019 ◽  
Vol 964 ◽  
pp. 109-114 ◽  
Author(s):  
Siti Zullaikah ◽  
Nizar Dwi Wibowo ◽  
I Made Gede Eris Dwi Wahyudi ◽  
M. Rachimoellah
Keyword(s):  
Hydrogen Bond ◽  
Rice Bran ◽  
Rice Bran Oil ◽  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Molar Ratio ◽  
Extraction Temperature ◽  
Hydrogen Bond Donor ◽  
Hydrogen Bond Acceptor ◽  
C 50

High content of free fatty acids (FFA) in crude rice bran oil (CRBO) needs to be separated through deacidification. Generally, deacidification process that is widely used are chemical and physical processes which causes the loss of bioactive compounds (γ-oryzanol) and un-environmentally friendly. The liquid-liquid extraction (LLE) using deep eutectic solvents (DES) to remove FFA and preserve g-oryzanol would be implemented in this study. DES with different hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA) with certain molar ratio such as Choline Chloride (ChCl)-Ethylene glycol 1:2 (DES I), ChCl-Glycerol 1:1 (DES II), ChCl-Urea 1:2 (DES III), ChCl-Oxalic acid 1:2 (DES IV), and Betaine Monohydrate-Glycerol 1:8 (NADES) were used as solvent to extract FFA from dewaxed/degummed RBO (DDRBO) for certain extraction time (30, 60, 120, 180, and 240 min) and extraction temperature (30°C, 40°C, 50°C, 60°C, and 70°C) under stirring (200 rpm). Deacidification using DES I for 240 min. and temperature of 50 °C was the optimum solvent to remove FFA (19.03 ± 2.33 %) and preserve g-oryzanol (recovery of g-oryzanol was 51.30 ± 1.77 %). The results also revealed that the longer time of extraction would be increased removal of FFA and decreased recovery of g-oryzanol. The higher temperature of extraction would be increased removal of FFA. In this work, temperature of 50 °C was the best extraction temperature of FFA since DES has highest solubility at this temperature.

scholarly journals Green and Efficient Extraction of Resveratrol from Peanut Roots Using Deep Eutectic Solvents

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Jingnan Chen ◽  
Xingxing Jiang ◽  
Guolong Yang ◽  
Yanlan Bi ◽  
Wei Liu
Keyword(s):  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Extraction Yield ◽  
Box Behnken Design ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Efficient Extraction ◽  
Ultrasonic Assisted Extraction ◽  
Solid Liquid ◽  
Total Extraction

Deep eutectic solvents (DESs), a new group of ecofriendly solvent combined with the ultrasonic-assisted extraction (UAE) technique, were first successfully used for extraction of resveratrol from peanut roots. Resveratrol in the extract was analyzed and quantified using a HPLC-UV method. A series of DESs consisting of choline chloride (ChCl) and 1,4-butanediol, citric acid, and ethylene glycol were formulated, finding ChCl/1,4-butanediol was a most proper extraction system. The optimal extraction parameters were obtained using a Box–Behnken design (BBD) test combined with response surface methodology as follows: 40% of water in ChCl/1,4-butanediol (1/3, g/g) at 55°C for 40 min and solid/liquid ratio of 1:30 g/mL. The total extraction content and extraction yield of resveratrol from peanut roots could reach 38.91 mg/kg and 88.19%, respectively, under such optimal conditions. The present study will provide a typical example for using DESs to extract natural bioactive compounds from plants.

scholarly journals Extraction of Polyphenols from Olive Leaves Employing Deep Eutectic Solvents: The Application of Chemometrics to a Quantitative Study on Antioxidant Compounds

2022 ◽  
Vol 12 (2) ◽  
pp. 831
Author(s):  
Hamida Akli ◽  
Spyros Grigorakis ◽  
Abdessamie Kellil ◽  
Sofia Loupassaki ◽  
Dimitris P. Makris ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Deep Eutectic Solvents ◽  
Extraction Process ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Extraction Temperature ◽  
Olive Leaves ◽  
Box Behnken Design ◽  
Solid Ratio

The extraction of phenolic compounds from olive leaves was optimized using three glycerol-based deep eutectic solvents (DESs) with lysine, proline, and arginine. A three-level Box–Behnken design was used to examine the influence of the liquid/solid ratio, concentration of DESs, and extraction temperature on the yield of the extraction process. A second-order polynomial model was used for predicting the polyphenol extraction yield. The optimal predicted conditions were used for extractions and they provided the highest total phenol yields with the glycerol–lysine exhibiting the best performance. Quantification of tyrosol, hydroxytyrosol, oleuropein, luteolin-7-O-glucoside, and rutin in the extracts showed high content in tyrosol in all DESs, particularly with glycerol–lysine and relatively similar contents with other studies for the other phenolic compounds. Finally, a linear relationship between tyrosol content and the total phenolic content of the extracts was observed.

scholarly journals Optimization Studies on the Extraction of Flavone Di-C-glycosides from Premna fulva Craib by Deep Eutectic Solvents

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Yueyuan Chen ◽  
Jiaoyang Dang ◽  
Xiaojie Yan ◽  
Fenglai Lu ◽  
Dianpeng Li
Keyword(s):  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Microstructural Alterations ◽  
Solid Ratio ◽  
Macroporous Resins ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Extraction Solvents ◽  
Ultrasonic Assisted Extraction ◽  
Novel Strategy

Deep eutectic solvents (DESs) are emerging as new extraction solvents for the extraction of bioactive compounds from natural plants. In this work, a total of thirteen choline chloride-based DESs were synthesized and evaluated for the simultaneous extraction of five bioactive flavone di-C-glycosides from Premna fulva Craib. DES-F, which was composed of choline chloride and 1,3-propanediol, showed the best extraction efficiency for the flavone di-C-glycosides, exceeding that of the conventional industrial solvent (40% ethanol). Subsequently, the extraction parameters for ultrasonic-assisted extraction (liquid-solid ratio, water content (%) in the selected DES, and extraction time) were optimized using response surface methodology. Furthermore, the microstructural alterations in the samples after extraction were evaluated using scanning electron microscopy. In addition, we further verified the recovery of flavone di-C-glycosides from the DESs using macroporous resins and evaluated the reusability of the DESs as solvents. The results indicated that DES-F was an efficient and sustainable solvent for the extraction of bioactive flavone di-C-glycosides from Premna fulva Craib. Therefore, this novel strategy may represent a sustainable approach for the extraction of bioactive products and could make phytochemistry more attractive and environmentally friendly.

scholarly journals Sambucus racemosa L. fruit extracts obtained with conventional and deep eutectic solvents

2020 ◽  
Vol 18 (2) ◽  
pp. 89-97
Author(s):  
Vojkan Miljkovic ◽  
Biljana Djordjevic ◽  
Biljana Arsic ◽  
Marija Markovic ◽  
Ljubisa Nikolic ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Choline Chloride ◽  
Radical Scavenging ◽  
Deep Eutectic Solvents ◽  
Extraction Yield ◽  
Free Radical Scavenging ◽  
Total Phenol Content ◽  
Total Flavonoids ◽  
Fruit Extracts

This study aimed to provide information on the extraction yields, total phenols, total flavonoids, antioxidant activity, and qualitative-quantitative chemical composition of the extracts obtained from the dried fruit of Sambucus racemosa L. with conventional solvents (methanol, acetone, chloroform) and deep eutectic solvents (choline chloride urea and choline chloride glycerol). Solid-liquid extraction, Folin- Ciocalteu method, DPPH free radical scavenging, HPLC-UV/VIS were the methods used in this study. The highest extraction yield (18%) was obtained with ChCl:G as an extragent. The highest amount of total flavonoids was the content of this extract (12.3 mg QE/g d.e.). Extracts obtained with acetone and ChCl:U possess the highest amount of total phenol content (125.26 and 104.38 mg GAE/g d.e. respectively). All tested extracts showed free radical scavenging activity. Identified components of extracts are gallic acid, rutin, caftaric acid, caffeic acid, hyperoside, vitexin, and quercetin. Fruit extracts of S. racemosa are a rich source of phenols and flavonoids. The extracts obtained with DESs are more active in free radical scavenging than those obtained with conventional solvents, and possess qualitatively and quantitatively more components, apart from vitexin that was detected only in the methanol extract.

scholarly journals Environmentally-Friendly Extraction of Flavonoids from Cyclocarya paliurus (Batal.) Iljinskaja Leaves with Deep Eutectic Solvents and Evaluation of Their Antioxidant Activities

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2110 ◽  
Author(s):  
Xianchao Shang ◽  
Jia-Neng Tan ◽  
Yongmei Du ◽  
Xinmin Liu ◽  
Zhongfeng Zhang
Keyword(s):  
Antioxidant Activities ◽  
Choline Chloride ◽  
Radical Scavenging ◽  
Deep Eutectic Solvent ◽  
Environmentally Friendly ◽  
Deep Eutectic Solvents ◽  
Selective Extraction ◽  
Molar Ratio ◽  
Cyclocarya Paliurus

Deep eutectic solvents (DESs) are commonly employed as environmentally-friendly solvents in numerous chemical applications owing to their unique physicochemical properties. In this study, a novel and environmentally-friendly extraction method based on ultrasound assisted-deep eutectic solvent extraction (UAE-DES) was investigated for the extraction of flavonoids from Cyclocarya paliurus (Batal.) Iljinskaja (C. paliurus) leaves, and the antioxidant activities of these flavonoids were evaluated. Nine different DES systems based on either two or three components were tested, and the choline chloride/1,4–butanediol system (1:5 molar ratio) was selected as the optimal system for maximizing the flavonoid extraction yields. Other extraction conditions required to achieve the maximum flavonoid extraction yields from the leaves of C. paliurus were as follows: DES water content (v/v), 30%; extraction time, 30 min; temperature, 60 °C; and solid-liquid ratio, 20 mg/mL. Liquid chromatography-mass spectrometry allowed the detection of five flavonoids in the extract, namely kaempferol-7-O-α-l-rhamnoside, kaempferol, quercetin, quercetin-3-O-β-d-glucuronide, and kaempferol-3-O-β-d-glucuronide. In vitro antioxidant tests revealed that the flavonoid-containing extract exhibited strong DPPH and ABTS radical-scavenging abilities. Results indicate that UAE-DES is a suitable approach for the selective extraction of flavonoids from C. paliurus leaves, and DESs can be employed as sustainable extraction media for other bioactive compounds.

Optimization of Ultrasonic-Assisted Extraction of Polysaccharides from Ottelia acuminata (Gagnep.) Dandy Using RSM

2011 ◽  
Vol 396-398 ◽  
pp. 292-296
Author(s):  
Ai Shi Zhu
Keyword(s):  
Processing Parameters ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Independent Variables ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Technology ◽  
Ultrasonic Assisted ◽  
Experimental Yield ◽  
Ultrasonic Assisted Extraction

Ultrasonic technology was applied to polysaccharides extraction from Ottelia acuminata (Gagnep.) Dandy and Response Surface Methodology (RSM) was used to optimize the effects of processing parameters on polysaccharides yields. Three independent variables such as liquid-solid ratio (ml/g, X1), extraction temperature (°C, X2) and extraction time (hour, X3) were investigated respectively. The statistical analysis indicated that the three variables and the quadratic of X1 and X3 had significant effects on the yields and followed by the significant interaction effects between the variables of X1 and X3, X2 and X3 (p<0.05). A mathematical model with high determination coefficient was gained. The optimal extraction conditions of polysaccharides were determined as follows: liquid-solid ratio 43 ml/g, extraction temperature 90 °C and extraction time 3.45 hours. Under these conditions, the experimental yield of polysaccharides was 107.44 mg/g, which was agreed closely with the predicted value 108.71 mg/g.

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