Selection Criteria for Drought-Tolerant Bread Wheat Genotypes at Seedling Stage

Diminishing water resources as a result of excessive use of water for irrigation and climate change posture a severe global threat to food security. Herein, an experiment was conducted to determine the selection criteria for drought-tolerant bread wheat genotypes at the seedling stage using morphological and photosynthetic pigmentation-related traits. A panel of 105 wheat landraces, historical Pakistani varieties, and advance breeding lines were evaluated under normal and drought stress using factorial completely randomized design. The root length, fresh weight, dry weight, cell membrane thermo-stability, and chlorophyll b were positively correlated among themselves under both normal and stress conditions. Hence, selection of any one of these traits enhances the performance of other traits. The shoot length was non-significant and negatively associated with all other studied characters except relative water content. The results suggested that selection for shoot length could not improve genetic gain for drought tolerance. Out of 10 principal components (PCs), the first three PCs were showed significant genetic variation under both conditions. The first three PCs showed 74.6% and 76% cumulative genetic variation under normal and drought conditions, respectively. Based on PCA, 10 drought-tolerant and five drought-susceptible genotypes were identified. Overall results suggested that selection for root length, fresh weight, dry weight, cell membrane thermo-stability, and chlorophyll b at the seedling stage would improve genetic gain for drought tolerance. The outperforming genotypes under drought stress conditions can be useful in future wheat breeding programs, and early selection for the traits recommended in this study will be effective for developing high-yielding and drought-tolerant wheat varieties.

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Allelopathic Potential of Haloxylon persicum against Wheat and Black Mustard with Special Reference to Its Phytochemical Composition and Antioxidant Activity

Agronomy ◽

10.3390/agronomy11020244 ◽

2021 ◽

Vol 11 (2) ◽

pp. 244

Author(s):

Ibrahim Bayoumi Abdel-Farid ◽

Mohamed Salah Massoud ◽

Yahia Al-Enazy ◽

Arafat Abdel Hamed Abdel Latef ◽

Muhammad Jahangir ◽

...

Keyword(s):

Antioxidant Activity ◽

Seed Germination ◽

Dry Weight ◽

Proline Content ◽

Shoot Length ◽

Chlorophyll B ◽

Fresh Weight ◽

Wheat Seedlings ◽

Extract Concentration ◽

Black Mustard

Haloxylon persicum is a well-known plant of sandy deserts, but yet, its allelopathic and phytochemical potential has not been explored. In the present study, we evaluated the effects of different concentrations (1, 2, 4, and 8%) of the aqueous extract of H. persicum shoots on seed germination and seedling growth of wheat crop and black mustard weed. The effects of the same extract on the biochemical parameters (chlorophyll a, chlorophyll b, carbohydrates content, and proline content) of wheat seedlings were also investigated. Low and moderate concentrations (1, 2, and 4%) of H. persicum aqueous extract nonsignificantly affected seed germination, shoot length, root length, fresh weight, and dry weight of wheat seedlings, whereas these parameters were negatively affected by the application of the highest extract concentration (8%). The results revealed a significant increase in proline content in wheat seedlings under all the applied concentrations of H. persicum extract. Low and moderate concentrations (1–4%) of H. persicum extract significantly enhanced chlorophyll a, chlorophyll b, and total chlorophyll content in wheat seedlings, whereas these parameters were significantly reduced when the highest extract concentration (8%) was applied. Only the lowest concentration (1%) of H. persicum extract reduced the carbohydrates content of wheat seedlings, while other concentrations showed nonsignificant effects. Concentrations of 4 and 8% of H. persicum extract reduced the seed germination percentage of black mustard. The root length, shoot length, and fresh weight of black mustard seedlings were significantly decreased by the application of 1 and 2% extract concentrations and completely inhibited in response to treatment with 4 and 8% concentrations. The dry weight of black mustard seedlings was not affected by 1 and 2% extract concentrations but completely inhibited at 4 and 8% concentrations. The methanol extract of H. persicum contains high contents of bioactive secondary metabolites, such as phenolics, flavonoids, flavonols, anthocyanins, tannins, saponins, as well as proved to has a very strong antioxidant activity of the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH). Based on our results, we recommend the potential application of the aqueous extracts of H. persicum to control black mustard weed in wheat crops. Furthermore, H. persicum revealed an interesting phytochemical pattern with sound antioxidant activity that assigns this plant as a promising candidate to explore its detailed metabolomics profile along with potential bioactivities.

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IDENTIFICATION OF DROUGHT TOLERANT WHEAT GENOTYPES UNDER WATER DEFICIT CONDITIONS

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v4.i2.2016.2830 ◽

2016 ◽

Vol 4 (2) ◽

pp. 206-214 ◽

Cited By ~ 1

Author(s):

Zaid Chachar ◽

N. A. Chachar ◽

Q.I. Chachar ◽

S.M Mujtaba ◽

G.A Chachar ◽

...

Keyword(s):

Water Stress ◽

Seed Germination ◽

Osmotic Stress ◽

Root Length ◽

Dry Weight ◽

Shoot Length ◽

Wheat Genotypes ◽

Maximum Reduction ◽

Drought Tolerant

Climate change is emerging phenomena and causing frequent drought which lead to scaricity of water, which ultimately nagetively affecting wheat (Triticumaestivum L.) yield all around the world. The aim of this study was to explore the potential deought tolerant wheat genotypes for candidate genes exploration. This study was conducted during the year 2014-2015 at Plant Physiology Division, Nuclear Institute of Agriculture (NIA) Tandojam. The six wheat genotypes (cv. MT-1/13, MT-2/13, MT-3/13, MT-4/13 Chakwal-86 and Khirman) were investigated for their response at germination and seedling stage under different water stress treatments (0, -0.5, -0.75 and -1.0 MPa) in controlled conditions. The results of experiments with reference to genotypes revealed that genotype Chakwal-86 shows maximum seed germination (82.58 %), while the genotype Khirman shows maximum shoot length (7.23 cm), root length (15.1 cm), shoot fresh wt. (5.85 g 10-1shoots), root fresh wt. (3.45 g 10-1roots), shoot dry wt. (1.33 g 10-1shoots), root dry wt. (0.69 g 10-1roots). Among the genotypes tested Khirman and MT-4/13 are the tolerant genotypes had the potential to perform better under drought conditions, whereas MT-4/13 and Chakwal-86 were moderate tolerant under water stress conditions. Moreover, the genotypes i.e. MT-1/13 and MT-2/13 are the sensitive genotypes under drought environment. It is concluded from present in-vitro studies that osmotic stress significantly reduced the seed germination shoot/root length fresh and dry weight in all six wheat genotypes. The maximum reduction was found at higher osmotic stress induced by PEG-6000 (-1.0 MPa) significantly.

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In vitro screening of rice genotypes using polyethylene glycol under drought stress

Progressive Agriculture ◽

10.3329/pa.v27i2.29321 ◽

2016 ◽

Vol 27 (2) ◽

pp. 128-135 ◽

Cited By ~ 1

Author(s):

J Akte ◽

S Yasmin ◽

MJH Bhuiyan ◽

F Khatun ◽

J Roy ◽

...

Keyword(s):

Water Stress ◽

Drought Stress ◽

Water Content ◽

Relative Water Content ◽

Dry Weight ◽

Proline Accumulation ◽

Shoot Length ◽

Fresh Weight ◽

Relative Water

Five rice varieties viz. Binadhan-4, Binadhan-5, Binadhan-6, Binadhan-10 and Iratom-24 were evaluated in vitro under different water stress conditions. Several parameters such as germination percentage, shoot length, root length, shoot-root ratio, fresh weight, dry weight, turgid weight, relative water content and proline accumulation were studied. Drought condition was created by MS medium supplemented with five treatments of PEG, with a control such as 0%, 1%, 2%, 3% and 4% of PEG. The highest germination (100%) was found in the variety Binadhan-10 under low water stress conditions induced by 1% PEG. Similarly, the highest percentage of germination was found in all varieties under control condition (0% PEG). The lowest percentage of germination was obtained in the variety Iratom-24. But under severe stress (4% PEG), the highest percentage of germination was found only in the variety Binadhan-10. Moreover, the variety Binadhan-10 was found to be the best at 4% PEG for shoot length, root length, shoot-root ratio, relative water content and also the best at 1% PEG for fresh weight, dry weight, turgid weight. Water stress decreased relative water content and increased proline accumulation in rice. The highest relative water content was recorded in the variety Binadhan-10 and the lowest value recorded in the variety Binadhan-5. The highest proline content was obtained from the binadhan-6 at the highest treatment (4% PEG). Binadhan-10 showed the best performance almost in all the parameters under drought stress because of its own nature of tolerancy.Progressive Agriculture 27 (2): 128-135, 2016

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Biosynthesis and characterization of silver nanoparticles using Ochradenus arabicus and their physiological effect on Maerua oblongifolia raised in vitro

Scientific Reports ◽

10.1038/s41598-020-74675-9 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Hassan O. Shaikhaldein ◽

Fahad Al-Qurainy ◽

Mohammad Nadeem ◽

Salim Khan ◽

Mohamed Tarroum ◽

...

Keyword(s):

Silver Nanoparticles ◽

Chlorophyll Content ◽

Total Protein ◽

Dry Weight ◽

Shoot Length ◽

Antioxidant Enzyme Activities ◽

Fresh Weight ◽

Wide Range ◽

Shoot Number

Abstract Silver nanoparticles (AgNPs) are presently the most commonly generated engineered nanomaterials and are found in a wide range of agro-commercial products. The present study was designed to synthesize AgNPs biologically using Ochradenus arabicus leaves and investigate their effect on the morphophysiological properties of Maerua oblongifolia raised in vitro. Physicochemical methods (ultraviolet–visible spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy were performed for characterization and for obtaining microphotographs of the AgNPs. Shoots of M. oblongifolia (2–3 cm) grown in Murashige and Skoog medium supplemented with different concentrations of AgNPs (0, 10, 20, 30, 40, or 50 mg L−1) were used. Following 6 weeks of in vitro shoot regeneration, the shoot number, shoot length, leaf number, fresh weight, dry weight, chlorophyll content, total protein, proline level, and antioxidant enzyme activities of the plants were quantified. We found that 20 mg L−1 AgNPs increased the shoot number, shoot length, fresh weight, dry weight, and chlorophyll content of the plants. The maximum total protein was recorded in plants that were administered the lowest dose of AgNPs (10 mg L−1), while high concentrations of AgNPs (40 and 50 mg L−1) increased the levels of proline and the enzymes superoxide dismutase and catalase. Our results indicate that green-synthesized AgNPs may be of agricultural and medicinal interest owing to their effects on plants in vitro.

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Effect of Water Extract Originated from Different Part of Russian Knapweed (Acroptilon repens L.) on Growth of Purslane (Portulaca oleracea L.)

Notulae Scientia Biologicae ◽

10.15835/nsb427572 ◽

2012 ◽

Vol 4 (2) ◽

pp. 108-111

Author(s):

Alireza PIRZAD ◽

Mousa JAMALI ◽

Mohammad Amin ZAREH ◽

Fahime SHOKRANI

Keyword(s):

Aerial Part ◽

Water Extract ◽

Dry Weight ◽

Shoot Length ◽

Portulaca Oleracea ◽

Control Treatment ◽

Fresh Weight ◽

Extract Concentration ◽

Russian Knapweed ◽

Seedling Length

To evaluate probable allelopathic effect of water extract originated from different parts of russian knapweed (Acroptilon repens L.) on growth of purslane (Portulaca oleracea L.), a factorial experiment was conducted based on randomized complete block design with three replications at greenhouse condition in 2011. Treatments were water extract concentrations (0, 5, 10, 15 and 20 percent) obtained from different part of russian knapweed (aerial part, flower and root). Results showed the significant effect of extract type on seedling length, and extract concentration on shoot length, root length and seedling length. Interaction effect between extract type and concentration on the ratio of root/shoot length, seedling fresh weight, seedling dry weight and germination percentage was significant, too. Means comparison indicated that the longest root (3.55 cm), shoot (4.65 cm) and seedling (8.20 cm) were obtained from control treatment, reducing with higher concentration of extract. The longest shoot (6.95 cm) belonged to extract originated from russian knapweed flowers. The highest ratio of root/shoot length (0.73), seedling fresh weight (0.61g) and seedling dry weight (0.044 g) belonged to control treatment. In general, increasing of extract concentration caused in reduction of germination and seedling traits. Meanwhile, extracts of aerial part had more sever reducing effect than root and flower extracts.

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Physiological response of chickpea (Cicer arietinum L.) at early seedling stage under salt stress conditions

Legume Research - An International Journal ◽

10.18805/lr-4059 ◽

2019 ◽

Author(s):

Anita Mann ◽

Gurpreet Kaur ◽

Ashwani Kumar ◽

Satish Kumar Sanwal ◽

Jogendra Singh ◽

...

Keyword(s):

Salt Stress ◽

Salt Tolerance ◽

Seedling Growth ◽

Root Length ◽

Dry Weight ◽

Shoot Length ◽

Seedling Stage ◽

Tolerance Index ◽

Early Seedling Growth ◽

Early Seedling

Screening of chickpea lines for salt tolerance through seed germination and early seedling growth is crucial for their evaluation. Seeds of 30 chickpea genotypes were germinated on a sand bed irrigated with saline (3, 6, 9, 12 dS/m) and control solutions upto 30 days. At the early seedling stage (25-30 days), germination percentage, chlorophyll content, proline, root length, shoot length and seedling dry weight were found to be affected due to salinity. Salt tolerance index (STI) for plant biomass maintained a significant correlation with chlorophyll, proline, shoot length, and root length, which indicated that these parameters could be used as selection criteria for screening chickpea genotypes against salt stress. Significant differences in shoot length, root length, and seedling dry weight in 30-day-old seedlings were observed among selected chickpea genotypes as well. From the overall observation of germination characterstics and early seedling growth, it is concluded that the chickpea genotypes, HC-1, HC-5, ICC 867, ICC 5003, H-10-41 showed better salt tolerance as compared to the available salt tolerant check variety.

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Salinity induced deleterious effects on germination, growth, physiological and biochemical process of two varieties of groundnut (Arachis hypogaea L.)

Archives of Agriculture and Environmental Science ◽

10.26832/24566632.2020.050209 ◽

2020 ◽

Vol 5 (2) ◽

pp. 144-150

Author(s):

Sharmin Akter ◽

Dilruba Yeasmin Jharna ◽

Sujan Kanti Mali ◽

Abu Sayeed

Keyword(s):

Salt Stress ◽

Chlorophyll Content ◽

Arachis Hypogaea ◽

Dry Weight ◽

Chlorophyll B ◽

Total Chlorophyll ◽

Fresh Weight ◽

Total Chlorophyll Content ◽

Arachis Hypogaea L ◽

Physiological And Biochemical

The present study was accomplished to find out the effects of salinity on germination, growth, physiological and biochemical processes of two different groundnuts (Arachis hypogaea L.), varieties viz., Dacca-1, and Zhinga groundnut. The experiments consisted of five salinity treatments viz., control (no salinity), 3, 6, 9, and 12dS/m during germination and vegetative stage of groundnut varieties. Results showed that germination percentage of both the groundnut varieties was significantly decreased with the increase of salt concentration. During the germination stage, maximum shoot and root length, shoot and root fresh weight and shoot and root dry weight of groundnut varieties were recorded from Zhinga groundnut variety at salt stress. The same result occurred during the vegetative stage of Dacca-1 variety while the Zhinga groundnut variety showed maximum parameters than Dacca-1. Fresh weight, as well as dry weight, of shoots and roots of both the groundnut varieties was also decreased with the increase of salt concentrations while leaf proline concentrations were increased among these varieties. Salinity caused significant (P<0.001) reduction in chlorophyll a, chlorophyll b, and total chlorophyll content in both the genotypes. Between two genotypes, Zhinga groundnut recorded higher chlorophyll a, chlorophyll b and total chlorophyll content than Dacca-1. From the studies, it is concluded that the groundnut genotype Zhinga was identified as the tolerant genotype to salt stress than Dacca-1. Overall results indicate that high salinity condition is not suitable for growing the groundnut.

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Salinity Tolerance of Mungbean Genotypes at Seedling Stage

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v10i2.13999 ◽

2018 ◽

Vol 10 (2) ◽

pp. 409-416

Author(s):

Ratri Tri Hapsari ◽

Trustinah Trustinah

Keyword(s):

Germination Rate ◽

Lateral Roots ◽

Block Design ◽

Dry Weight ◽

Germination Percentage ◽

Seedling Stage ◽

Fresh Weight ◽

Vigor Index ◽

Normal Seedling ◽

Two Factors

Salinity is a major abiotic stress limiting mungbean production worldwide including Indonesia. Since mungbean plant is very sensitive to salt condition, selection of salinity tolerant genotypes becomes important for mungbean improvement. The objective of this study was to evaluate the tolerance of eight mungbean genotypes to salinity at seedling stage under different levels. The experiment was arranged in a randomized complete block design with two factors (mungbean genotypes and salinity levels) and triplicates. Observation variables were germination percentage, vigor index, germination rate, hypocotyls length, epicotyls length, root length, number of root, seedling fresh weight, and seedling dry weight. The result showed that increasing level of salinity concentration inhibited the speed of germination, germination percentage, vigor index, normal seedling fresh weight, and number of lateral roots. Murai and Vima 1 were identified as tolerant genotypes, while Vima-2 and MLGV 0180 were identified as salinity sensitive genotypes at seedling stage. Currently, mungbean varieties with special characters, such as saline-tolerant is not yet available. The availability of saline-tolerant variety of mungbean is a cheaper and easier technology for farmers to anticipate the expansion of the saline area. The tolerant genotypes may be further tested at the later stage to obtain promising genotype tolerant to salinity that effectively assist mungbean breeding program.

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Growth Effects of Uniconazole, Gibberellin, and Calcium on Salt Injuries of Hibiscus syriacus and H. hamabo seedlings

HortScience ◽

10.21273/hortsci.30.4.899d ◽

1995 ◽

Vol 30 (4) ◽

pp. 899D-899

Author(s):

Moo R. Huh ◽

Beyoung H. Kwack ◽

Leonard P. Perry

Keyword(s):

Root Length ◽

Weight Ratio ◽

Dry Weight ◽

Leaf Length ◽

Shoot Length ◽

Leaf Width ◽

Fresh Weight ◽

Crown Diameter ◽

Nacl Concentration ◽

Hibiscus Syriacus

Shoot length, leaf length and width, root length, and crown diameter were affected by 0.0-, 0.5-, 1.0-, and 5.0-mg·liter–1 uniconazole drench and 25- or 50-mg·liter–1 GA3 spray but not significantly by 0.0%, 2.5%, and 5.0% NaCl. Leaf width of H. syriacus Sieb. & Zucc. was not affected and that of H. syriacus L. significantly decreased, as NaCl concentration increased. Effect of NaCl on H. syriacus leaf width was offset by treatment with uniconazole but not by GA3 treatment. With 2.5% NaCl, dry weight of H. hamabo treated with uniconazole or GA3 increased and that of treated H. syriacus decreased. With 5.0% NaCl, dry weight of both species decreased with uniconazole or GA3. Calcium at 13.35 or 133.5 mM decreased the reduction of dry weight by NaCl treatment. The dry: fresh weight ratio of H. hamabo and H. syriacus treated with NaCl plus uniconazole was higher than that only treated with NaCl. GA3 treatment with NaCl did not affect the dry: fresh weight ratio for either species

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Growth Assessment of Tropical Sugarbeet as Influenced by Spacing

Bangladesh Agronomy Journal ◽

10.3329/baj.v23i2.52452 ◽

2021 ◽

Vol 23 (2) ◽

pp. 51-58

Author(s):

MAT Sohel ◽

MAE Hossain ◽

HP Roy ◽

SM Reza ◽

FH Shanta ◽

...

Keyword(s):

Root Length ◽

Block Design ◽

Dry Weight ◽

Germination Percentage ◽

Research Field ◽

Shoot Length ◽

Fresh Weight ◽

Shoot Fresh Weight ◽

Number Of Leaves ◽

Maximum Root

The experiment was carried out at the research field of Agronomy and Farming Systems Division, Bangladesh Sugarcrop Research Institute (BSRI), Ishurdi, Pabna during 2012-2013 to determine the most suitable spacing for sugarbeet cultivation in Bangladesh. The experiment was conducted with nine spacing viz. 50 cm × 20 cm, 60 cm × 20 cm, 70 cm × 20 cm, 50 cm × 25 cm, 60 cm × 25 cm, 70 cm × 25 cm, 50 cm × 30 cm, 60 cm × 30 cm and 70 cm × 30 cm in a randomized complete block design with three replications. The effects of spacing on sugarbeet plantation were observed on growth and growth contributing components (germination percentage, number of leaves plant-1, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, crop growth rate) of sugarbeet. The highest germination percentage (95.67%), number of leaves plant-1 (34.33) at 30 DAS, shoot length (54.07 cm) at 120 DAS, root fresh weight (969.47 g plant-1) at 150 DAS, shoot fresh weight (752.47 g plant-1) at 120 DAS and other growth contributing parameters were obtained with the spacing 70 cm × 30 cm. However, the maximum root length (38.97 cm) was obtained with 50 cm × 20 cm spacing. It was concluded that the wider spacing promoted the growth of individual beet, though the optimum spacing for maximum root growth of sugarbeet was 50 cm × 20 cm. Bangladesh Agron. J. 2020, 23(2): 51-58

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