scholarly journals Effects of Combined Nitrogen Deficient and Mixotrophic (+Glucose) Culture on the Lipid Accumulation of Parachlorella Kessleri TY

Water ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3066
Author(s):  
Yifan Gao ◽  
Li Ji ◽  
Jia Feng ◽  
Junping Lv ◽  
Shulian Xie
Keyword(s):  
Lipid Accumulation ◽  
Large Scale ◽  
Neutral Lipids ◽  
Lipid Production ◽  
Production Capacity ◽  
Raw Material ◽  
Shanxi Province ◽  
Scale Production ◽  
Parachlorella Kessleri ◽  
Accumulation Ability

The combustion explosion of ordinary diesel is a global environmental problem. Green microalgae, which do not cause eutrophication, are a raw material that can be used to clean biodiesel. To increase lipid productivity, this study used a nitrogen deficient & mixotrophic (+Glucose) culture of lipid-producing microalgae Parachlorella kessleri TY from Shanxi Province, China. To examine the growth of P. kessleri TY, we measured dry weight, chlorophyll content, and chlorophyll fluorescence intensity under different culture conditions, in addition to the contents of neutral lipids, total lipids, and fatty acids, to examine its lipid accumulation ability. Cells were cultured in autotrophic, nitrogen deficient, mixotrophic (+Glucose), and nitrogen deficient & mixotrophic (+Glucose) conditions for 7 days. We found the growth of P. kessleri TY under nitrogen deficient & mixotrophic conditions was higher than that under the autotrophic and nitrogen deficient conditions, but lower than that under the mixotrophic (+Glucose) conditions. However, its lipid accumulation ability was significantly higher than that of control cultures. In conclusion, P. kessleri TY cultured under nitrogen-deficient and mixotrophic (+Glucose) conditions has significant lipid production capacity. Our results provide a theoretical basis for the use of microalgae as a raw material in the production of biodiesel, and promote the application of P. kessleri TY in large-scale production.

scholarly journals Lipid accumulation of Chlorella sp. TLD6B from the Taklimakan Desert under salt stress

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11525
Author(s):  
Hong Li ◽  
Jun Tan ◽  
Yun Mu ◽  
Jianfeng Gao
Keyword(s):  
Salt Stress ◽  
Fatty Acid ◽  
Lipid Accumulation ◽  
Large Scale ◽  
Fatty Acid Biosynthesis ◽  
High Salinity ◽  
Raw Material ◽  
Biodiesel Production ◽  
Taklimakan Desert ◽  
Acid Biosynthesis

Chlorella has become an important raw material for biodiesel production in recent years, and Chlorella sp. TLD6B, a species with high lipid concentrations and high salt and drought tolerance, has been cultivated on a large scale. To explore the lipid accumulation of Chlorella sp. TLD6B and its relationship to external NaCl concentrations, we performed physiological measurements and genome-wide gene expression profiling under different levels of salt stress. Chlorella sp. TLD6B was able to tolerate high levels of salt stress (0.8 M NaCl addition). Lipid concentrations initially increased and then decreased as salt stress increased and were highest under the addition of 0.2 M NaCl. Comparative transcriptomic analysis revealed that salt stress enhanced the expression of genes related to sugar metabolism and fatty acid biosynthesis (the ACCases BC and BCCP, KAS II, and GPDHs involved in TAG synthesis), thereby promoting lipid accumulation under the addition of 0.2 M NaCl. However, high salinity inhibited cell growth. Expression of three SADs, whose encoded products function in unsaturated fatty acid biosynthesis, was up-regulated under high salinity (0.8 M NaCl addition). This research clarifies the relationship between salt tolerance and lipid accumulation and promotes the utilization of Chlorella sp. TLD6B.

Optimal Design and Techno-Economic Analysis for Corncob Particles Briquettes: A Literature Review of the Utilization of Agricultural Waste and Analysis Calculation

2021 ◽  
Author(s):  
Asep Bayu Dani Nandiyanto ◽  
Nissa Nur Azizah ◽  
Gabriela Chelvina Santiuly Girsang
Keyword(s):  
Optimal Design ◽  
Literature Review ◽  
Economic Analysis ◽  
Large Scale ◽  
Net Present Value ◽  
Raw Materials ◽  
Agricultural Waste ◽  
Raw Material ◽  
Variable Cost ◽  
Scale Production

Corncob is usually disposed of directly as waste, creating problems in the environment, while it can be converted into valuable materials. This research aimed to evaluate the literature review on briquette production from agricultural waste (using non-binder and cold press with a binder) and the current works on techno-economic analysis, to propose an optimal design for the production of briquette from corncob waste, and to perform a techno-economic analysis based on the selected optimal processing method. The engineering perspective based on stoichiometry and mass balance showed the potential corncob briquette manufacture in both home and large scales due to the possible use of inexpensive and commercially available equipment and raw materials. The economic perspective [based on several economic evaluation factors (i.e., gross profit margin, payback period, break-even point, cumulative net present value, return of investment, internal rate return, and profitability index) under ideal and non-ideal conditions by considering internal (i.e., sales, raw materials, utilities, and variable cost) and external aspects (i.e., tax)] confirmed the prospective development of the project in the large-scale production with a lifetime of more than 18 years. The main issue in the project is the raw material (i.e. tapioca flour), giving the most impact on the project’s feasibility. Even in severe conditions, the project is feasible. The great endurance was also confirmed in the case of a higher tax rate. This study demonstrates the importance of producing corncob-based briquettes for improving the economic value and giving alternatives for problem solvers in the utilization of agricultural waste.

WASTE FRYING OIL TRANSESTERIFICATION TREATED BY STEAM DRAG METHOD

2018 ◽  
Vol 26 (26) ◽  
pp. 34-42
Author(s):  
Daniel Sena MARINS ◽  
Marcos Vinícius Oliveira CARDOSO ◽  
Mara Eliza SANTOS ◽  
Jeferson MASSINHAN
Keyword(s):  
Moisture Content ◽  
Conversion Rate ◽  
Large Scale ◽  
Methyl Esters ◽  
Frying Oil ◽  
Raw Material ◽  
Biodiesel Production ◽  
Scale Production ◽  
Residual Oil ◽  
Conversion Rates

Demand for diversified biodiesel feedstocks is high and increasing, but few are viable for large-scale production, and many of those selected compete with other sectors of the chemical industry. To improve energy and environmental sustainability, fatty acids from waste oils that are improperly disposed of and pollute the environment can be used for transesterification reactions. However, they need treatment to achieve high conversion rates. In this context, the aim of this work was to perform and analyze the treatment of residual frying oil with the evaporation and entrainment process, aiming at its use as raw material to obtain biodiesel (methyl esters) by a transesterification reaction. The physicochemical properties of the residual oil after treatment were characterized by moisture content, pH and the acidity, saponification, iodine, and peroxide index. The conversion rate of the residual oil to methyl esters was determined by 1H NMR analysis. After the treatment, the method of analysis of variance showed that the oil obtained a significant reduction of the saponification, iodine, peroxide and acidity indexes, being the acidity reduced from 9.36 to 7.85 mg KOH g-1. The moisture content of 0.733% and elevation of pH to 8.0. The conversion rate of fatty acid biodiesel of residual oil was 79.3 %, lower value of standards norms (ASTM, 2005; EN, 2008; ANP, 2014), showing that the assigned methodology for frying residual oil is inefficient in biodiesel production.

scholarly journals Biomass and lipid induction strategies in microalgae for biofuel production and other applications

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Hossein Alishah Aratboni ◽  
Nahid Rafiei ◽  
Raul Garcia-Granados ◽  
Abbas Alemzadeh ◽  
José Rubén Morones-Ramírez
Keyword(s):  
Lipid Content ◽  
Large Scale ◽  
Fossil Fuels ◽  
Low Cost ◽  
Lipid Production ◽  
Arable Land ◽  
Economic Cost ◽  
Biofuel Production ◽  
Scale Production ◽  
Microalgae Biomass

Abstract The use of fossil fuels has been strongly related to critical problems currently affecting society, such as: global warming, global greenhouse effects and pollution. These problems have affected the homeostasis of living organisms worldwide at an alarming rate. Due to this, it is imperative to look for alternatives to the use of fossil fuels and one of the relevant substitutes are biofuels. There are different types of biofuels (categories and generations) that have been previously explored, but recently, the use of microalgae has been strongly considered for the production of biofuels since they present a series of advantages over other biofuel production sources: (a) they don’t need arable land to grow and therefore do not compete with food crops (like biofuels produced from corn, sugar cane and other plants) and; (b) they exhibit rapid biomass production containing high oil contents, at least 15 to 20 times higher than land based oleaginous crops. Hence, these unicellular photosynthetic microorganisms have received great attention from researches to use them in the large-scale production of biofuels. However, one disadvantage of using microalgae is the high economic cost due to the low-yields of lipid content in the microalgae biomass. Thus, development of different methods to enhance microalgae biomass, as well as lipid content in the microalgae cells, would lead to the development of a sustainable low-cost process to produce biofuels. Within the last 10 years, many studies have reported different methods and strategies to induce lipid production to obtain higher lipid accumulation in the biomass of microalgae cells; however, there is not a comprehensive review in the literature that highlights, compares and discusses these strategies. Here, we review these strategies which include modulating light intensity in cultures, controlling and varying CO2 levels and temperature, inducing nutrient starvation in the culture, the implementation of stress by incorporating heavy metal or inducing a high salinity condition, and the use of metabolic and genetic engineering techniques coupled with nanotechnology.

scholarly journals Bovine Colostrum for Human Consumption - Improving Microbial Quality and Maintaining Bioactive Characteristics through Processing

2021 ◽  
Author(s):  
Sylvia Fasse ◽  
Jarmo Alarinta ◽  
Björn Frahm ◽  
Gun Wirtanen
Keyword(s):  
Heat Treatment ◽  
Large Scale ◽  
Bovine Colostrum ◽  
Raw Material ◽  
Human Consumption ◽  
Microbial Quality ◽  
Scale Production ◽  
Human Pathogens ◽  
Spoilage Bacteria

The purpose of bovine colostrum, being the milk secreted by a cow after giving birth, is to transfer passive immunity to the calf. The calves have an insufficient immune system as they lack immunoglobulins (Igs). Subsequently, the supply of good quality bovine colostrum is obligatory. The quality of colostrum is classified by low bacterial counts and adequate Ig concentrations. Bacterial contamination can contain a variety of human pathogens or high counts of spoilage bacteria, which becomes more challenging with emerging use of bovine colostrum as food and food supplements. There is also a growing risk for the spread of zoonotic diseases originating from bovines. For this reason, processing based on heat treatment or other feasible techniques are required. This review provides an overview of literature on the microbial quality of bovine colostrum and processing methods to improve its microbial quality and keep its nutritional values as food. The highlights of this review are: high quality colostrum is a valuable raw material in food products and supplements, the microbial safety of bovine colostrum is increased using appropriate processing, suitable effective heat-treatment, which does not destroy the high nutrition value of colostrum, the heat treatment processes are cost-effective compared to other methods, and heat treatment can be performed in both small- and large-scale production

scholarly journals Effect of biostimulant application on production and flavonoid content of marigold (Calendula officinalis L.)

Revista CERES ◽  
2014 ◽  
Vol 61 (6) ◽  
pp. 983-988 ◽  
Author(s):  
Vivian Pupo de Oliveira Machado ◽  
Ana Claudia Pacheco ◽  
Marcia Eugenia Amaral Carvalho
Keyword(s):  
Large Scale ◽  
Dry Mass ◽  
Raw Material ◽  
Growth Promoter ◽  
Scale Production ◽  
Flavonoid Content ◽  
Randomized Design ◽  
Large Scale Production ◽  
Number Of Leaves ◽  
Calendula Officinalis L

The production of medicinal plants as raw material for industry must associate quality with biomass formation and, with this purpose, the application of plant growth regulators has been studied in these crops. The objective of this study was to evaluate the effect of a biostimulant on growth, inflorescence production and flavonoid content in marigold. The experiment was conducted in a greenhouse and the treatments consisted of increasing doses of the biostimulant (0, 3, 6, 9, 12 and 15 mL L-1) applied by foliar spraying in ten consecutive applications. The experiment was arranged in a completely randomized design, with six treatments and ten repetitions. The number of leaves and flowerheads and dry matter of roots increased linearly with increasing doses of the growth promoter, with 20%, 36.97% and 97.28% increases, respectively, compared with the control. The total dry mass and shoot dry mass showed maximum values at the highest dose tested of 15 mL L-1 (with increases of 40.09% and 46.30%, respectively). Plant height and flavonoid content reached the highest values at a dose of 6 mL L-1. The biostimulant promoted the development of marigold and positively influenced the synthesis of the secondary compound of medicinal interest. Among the tested doses, the application of rates between 6 and 9 mL L-1 of the biostimulant is recommended for more efficient large-scale production of marigold.

scholarly journals Waste Frying Oil Transesterification Treated by Steam Drag Method

2018 ◽  
Vol 26 (26) ◽  
pp. 34-42
Author(s):  
Daniel Sena Marins ◽  
Marcos Vinícius Oliveira Cardoso ◽  
Mara Eliza Santos ◽  
Jeferson Massinhan
Keyword(s):  
Moisture Content ◽  
Conversion Rate ◽  
Large Scale ◽  
Methyl Esters ◽  
Frying Oil ◽  
Raw Material ◽  
Biodiesel Production ◽  
Scale Production ◽  
Residual Oil ◽  
Conversion Rates

Demand for diversified biodiesel feedstocks is high and increasing, but few are viable for large-scale production, and many of those selected compete with other sectors of the chemical industry. To improve energy and environmental sustainability, fatty acids from waste oils that are improperly disposed of and pollute the environment can be used for transesterification reactions. However, they need treatment to achieve high conversion rates. In this context, the aim of this work was to perform and analyze the treatment of residual frying oil with the evaporation and entrainment process, aiming at its use as raw material to obtain biodiesel (methyl esters) by a transesterification reaction. The physicochemical properties of the residual oil after treatment were characterized by moisture content, pH and the acidity, saponification, iodine, and peroxide index. The conversion rate of the residual oil to methyl esters was determined by 1H NMR analysis. After the treatment, the method of analysis of variance showed that the oil obtained a significant reduction of the saponification, iodine, peroxide and acidity indexes, being the acidity reduced from 9.36 to 7.85 mg KOH g-1. The moisture content of 0.733 % and elevation of pH to 8.0. The conversion rate of fatty acid biodiesel of residual oil was 79.3 %, lower value of standards norms (ASTM, 2005; EN, 2008; ANP, 2014), showing that the assigned methodology for frying residual oil is inefficient in biodiesel production

scholarly journals Biosurfactants – Nature's Solution for Today's Cleaning Challenges

2021 ◽  
Vol 75 (9) ◽  
pp. 752-756
Author(s):  
Jakob J. Mueller ◽  
Hans H. Wenk
Keyword(s):  
Large Scale ◽  
Raw Materials ◽  
Biological Molecules ◽  
Raw Material ◽  
Scale Production ◽  
Sustainable Solutions ◽  
Natural Substances ◽  
Head Groups ◽  
Large Scale Production ◽  
Recent Developments

Biosurfactants are surface-active molecules, developed by nature through evolution and naturally produced by different microorganisms. The most prominent examples are rhamnolipids and sophorolipids, molecules which contain hydrophilic sugar head groups and hydrophobic alkyl residues leading to an amphiphilic behavior with unique properties. Recent developments in the field of biotechnology enable the large-scale production of these biological molecules. The raw material basis is 100% renewable since sugars and oils are used as major raw materials. Additionally, biosurfactants are fully biodegradable, which allows the path back into the natural cycles. In comparison to established standard surfactants like SLES/SLS (sodium laureth (ether) sulfates) or betaines, rhamnolipids are much milder and, at the same time, show similar or even better performance in household or personal care applications. Foam behavior, solubilization and cleaning effectiveness are examples where these natural substances give excellent results compared to the synthetic benchmarks. The commercialization of biosurfactants at industrial scale now offers alternatives to consumers seeking sustainable solutions, without compromising performance. Biosurfactants combine both and set a new standard for surfactant applications.

scholarly journals Environmental and Economic Analysis of FDM, SLS and MJF Additive Manufacturing Technologies

Materials ◽  
2019 ◽  
Vol 12 (24) ◽  
pp. 4161 ◽  
Author(s):  
Vincenzo Tagliaferri ◽  
Federica Trovalusci ◽  
Stefano Guarino ◽  
Simone Venettacci
Keyword(s):  
Additive Manufacturing ◽  
High Performance ◽  
Large Scale ◽  
Fused Deposition Modeling ◽  
Production Capacity ◽  
Optimal Choice ◽  
Scale Production ◽  
Fused Deposition ◽  
Manufacturing Technologies ◽  
The Impact

In this study, the authors present a comparative analysis of different additive manufacturing (AM) technologies for high-performance components. Four 3D printers, currently available on the Italian national manufacturing market and belonging to three different AM technologies, were considered. The analysis focused on technical aspects to highlight the characteristics and performance limits of each technology, economic aspects to allow for an assessment of the costs associated with the different processes, and environmental aspects to focus on the impact of the production cycles associated with these technologies on the ecosystem, resources and human health. This study highlighted the current limits of additive manufacturing technologies in terms of production capacity in the case of large-scale production of plastic components, especially large ones. At the same time, this study highlights how the geometry of the object to be developed greatly influences the optimal choice between the various AM technologies, in both technological and economic terms. Fused deposition modeling (FDM) is the technology that exhibits the greatest limitations hindering mass production due to production times and costs, but also due to the associated environmental impact.

Kiruna-Type Ore as a Novel Precursor for Large-Scale Production of Small Uniform Iron Oxide Nanoparticles

2020 ◽  
Vol 20 (10) ◽  
pp. 6525-6531
Author(s):  
Majid Mostaghelchi ◽  
Jani Kotakoski ◽  
Christian Rentenberger ◽  
Christian L. Lengauer
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Large Scale ◽  
Critical Size ◽  
Raw Material ◽  
Oxide Nanoparticles ◽  
Scale Production ◽  
X Ray ◽  
Mechanical Attrition ◽  
Wide Range

The wide range of actual and potential applications of nanoparticles, highlight the necessity of a reliable production method for both quality and quantity of the products. Mechanical attrition is one of the first well-known techniques used to produce nanoparticles. However, these approaches have been restricted to produce uniform particles below the critical size of 15 nm because of the attrition balance limit. This paper introduces the magnetite–silicate raw material of a Kiruna-type ore deposit as a novel precursor, which enables the production of small iron oxide nanoparticles below the critical size by mechanical attrition. X-ray fluorescence (XRF), powder X-ray diffractometry (pXRD), dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used for characterization of the precursor and obtained nanoparticles. The results indicate that the particles with a mean diameter of 10.7(2.7) nm consist of mainly less than one crystallite. The significant size reduction below the attrition balance limit can be attributed to the quartz content of the raw material, which operated as supporting micro-balls for transferring the energy during the milling process.

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