scholarly journals Incidence and Antibiotic Resistance Properties of Campylobacter Species Isolated From Poultry Meat

2020 ◽  
Vol 8 (2) ◽  
pp. 60-65
Author(s):  
Ali Sabzmeydani ◽  
Ebrahim Rahimi ◽  
Amir Shakerian
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Nalidixic Acid ◽  
Poultry Meat ◽  
Diffusion Method ◽  
Contamination Rate ◽  
Wild Duck ◽  
Campylobacter Species ◽  
Meat Samples ◽  
Campylobacter Spp

Background: F Campylobacter species are imperative foodborne bacteria because of the contaminated poultry meat consumption. Objectives: This study was conducted to recognize the incidence and antimicrobial resistance profile of Campylobacter species recovered from raw poultry meat samples. Materials and Methods: A total of 695 poultry meat samples were collected and assessed by culture technique. Bacterial species were identified by polymerase chain reaction (PCR). Antimicrobial resistance was assessed by disk diffusion method (DDM). Results: The contamination rate of samples with Campylobacter spp. was 44.75% with higher contamination rate of wild duck (84%), wild goose (83.33%), coot (78.26%), chicken (67.78%), and wild pheasant (66.66%), respectively. Campylobacter jejuni and C. coli bacteria were found in 84.24% and 15.76% of Campylobacter spp., respectively. The highest incidence of C. jejuni was obtained in partridge (95.45%), quail (95%), pheasant (92.31%), and wild duck (90.48%) meat samples, respectively. The highest incidence of C. coli was found in turkey (52.63%) and wild pheasant (22.22%) meat samples, respectively. Moreover, C. jejuni had the highest resistance to tetracycline (76.34%), nalidixic acid (65.65%), ciprofloxacin (58.78%), enrofloxacin (39.69%), and ampicillin (38.55%), respectively. C. coli had the highest resistance to nalidixic acid (48.99%), ciprofloxacin (40.82%), and enrofloxacin (38.78%), respectively. Conclusion: Poultry meat, particularly partridge, quail, pheasant, turkey, and wild avian are the main sources of Campylobacter transmission. Furthermore, higher incidence and antibiotic resistance of C. jejuni was found. Proper cooking of poultry meat and monitoring the antibiotic prescription can lessen the occurrence of antibiotic-resistant Campylobacter spp. in poultry meat.

Prevalence and Antimicrobial Resistance of Listeria monocytogenes Isolated in Chicken Slaughterhouses in Northern Greece

2011 ◽  
Vol 74 (6) ◽  
pp. 1017-1021 ◽  
Author(s):  
I. SAKARIDIS ◽  
N. SOULTOS ◽  
E. IOSSIFIDOU ◽  
A. PAPA ◽  
I. AMBROSIADIS ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Nalidixic Acid ◽  
Random Amplified Polymorphic Dna ◽  
The Other ◽  
Diffusion Method ◽  
Northern Greece ◽  
Disk Diffusion Method ◽  
High Prevalence

This study was conducted to determine the prevalence and antimicrobial resistance of Listeria monocytogenes recovered from chicken carcasses in slaughterhouses in Northern Greece. A total of 100 poultry samples (300 carcasses) were examined for Listeria spp. The samples were neck skin taken from four different slaughterhouses in Northern Greece. Forty samples were also taken from the environment of the slaughterhouses. Identification of L. monocytogenes was carried out by PCR and fingerprinting of the isolates by random amplified polymorphic DNA. L. monocytogenes strains isolated from chicken carcasses and from the environment of the slaughterhouses were also examined for antibiotic resistance. Fifty-five isolates of L. monocytogenes were tested for susceptibility to 20 antibiotics using the disk diffusion method. Listeria spp. were present in 99 of the poultry samples tested (99%), and 38 yielded L monocytogenes (38%). L. monocytogenes was also isolated in 80% of samples from the environment of a certain slaughterhouse, while the other slaughterhouses were found to be contaminated only with Listeria spp. All isolates were resistant to nalidixic acid and oxolinic acid, the majority of them to clindamycin, and only a few to tetracycline and oxytetracycline, whereas they were found to be susceptible to all other antimicrobials. The results of this study demonstrate a high prevalence of L. monocytogenes contamination in chicken carcasses, and all isolates were found to be sensitive to the antimicrobials most commonly used to treat human listeriosis.

Prevalence and antibiotic susceptibility pattern of Campylobacter species isolated from broiler chicken meat samples in district Bannu, Pakistan

2021 ◽  
Author(s):  
Inam Ullah ◽  
Hajra Khurshid ◽  
Nadeem Ullah ◽  
Israr Aziz ◽  
Muhammad Junaid Khan ◽  
...  
Keyword(s):  
Public Health ◽  
Developed Countries ◽  
Cloacal Swab ◽  
Poultry Meat ◽  
Diffusion Method ◽  
Broiler Meat ◽  
Cross Sectional ◽  
Khyber Pakhtunkhwa ◽  
Campylobacter Species ◽  
Meat Samples

The campylobacter genus of bacteria is important in public health as it comprises many species causing diarrhea in humans. Poultry and their products are recognized as vital causes of campylobacteriosis in humans. For bacterial food-borne diseases, Campylobacter is considered as the leading cause. Higher prevalence has been reported in developed countries. Our study was a cross-sectional study directed to determine the prevalence of Campylobacter species in retail broiler meat in the Bannu district of Khyber Pakhtunkhwa, Pakistan, from January to June 2018. A total of 200 poultry meat samples were collected from four different areas of district Bannu Khyber Pakhtunkhwa province of Pakistan that includes Lakki gate, Tanchi bazar, Bannu Township and Mangal milla. Mueller-Hinton medium was used for disc diffusion method to determine antibiotic resistance of Campylobacter species. Amongst 200 broiler meat samples, 60 (30%) samples were found positive for Campylobacter species. The highest prevalence was observed in samples from Bannu Township (50%) while lowest prevalence (12%) was observed in samples from Mangal milla broiler meat samples. Amongst different types of meat samples, highest prevalence was found in thigh meat (46%), while lowest prevalence was observed in cloacal swab (20%). Highest resistance was observed against Amoxicillin (AMX) 80% while the resistance observed against other antibiotics were Ampicillin (AMP) 70%, Tetracycline (TET) 65%, Sulphamethoxazole + Trimethoprim (SXT) 60%, Chloramphenicol (CHL) 56.66%, Clarithromycin (CLR) 50%, Streptomycin (STR) 40%, Gentamycin (GEN) 36.66%, Ofloxacin (OFX) 20%, Ciprofloxacin (CIP) 15%, Levofloxacin (LEV) 15% and Azithromycin (AZM) 10%. The lowest resistance was observed against Ceftriaxone (CRO) 5%. Our Study concludes that Campylobacter species is prevalent highly in district Bannu, and it might be a hazard to public health.

scholarly journals Antimicrobial resistance Salmonella isolates recovered from food products of animal origin in the Russian Federation

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Galina Skitovich ◽  
Kseniya Serova ◽  
Yevgenia Korchagina ◽  
Natalya Shadrova
Keyword(s):  
Antibiotic Resistance ◽  
Nalidixic Acid ◽  
Acid Resistance ◽  
Food Products ◽  
Poultry Meat ◽  
Animal Origin ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
The Russian Federation ◽  
Nalidixic Acid Resistance

The study was aimed at Salmonella isolation from samples of animal food products submitted for testing from various regions of the Central part of the RF and serotyping of the recovered isolates and their testing for antibiotic resistance. A total of 2,342 tests were performed and 87 (3.7%) Salmonella isolates were recovered. Most of them (54 isolates) were recovered from poultry meat and poultry meat preparation samples submitted for testing. Besides, 25 isolates were recovered from pork and pork preparation samples, 7 isolates – from beef samples, 1 isolate – from hard cheese samples. Serotyping of 64 Salmonella isolates showed that the majority of the isolates (57.8%) belonged to О7 group. Also, Salmonella isolates belonging to О9 (21.9%), О8 (9.4%), О4,5 (6.2%) and О10 (4.7%) were detected in food products. S. Enteritidis, (23.3%), and S. infantis (18.7%), were predominant based on the number of detections. Also, the following serovars were identified: S. typhimurium, S. nigeria, S. montevideo, S. derby, S. meleagridis, S. virchov, S. oranienburg. Tests of 87 Salmonella isolates for their antibiotic resistance with disk diffusion method revealed that they were highly resistant to nalidixic acid (70.1%), tetracycline (49.4%), trimethoprim/sulfamethoxazol (40.2%). Moreover, nalidixic acid-resistance was common for all identified isolates. Seventeen isolates (19.5%) demonstrated multiple antibiotic resistance and two isolates were found to be resistant to ≥7 antibiotics. All recovered isolates were susceptible to gentamicin, amikacin, meropenem and imipenem. Obtained results indicate the necessity of Salmonella antibiotic resistance monitoring to gain understanding of Salmonellas’ antibiotic resistance emergence and trends.

scholarly journals Prevalence, virulence factor and antimicrobial resistance analysis of Salmonella Enteritidis from poultry and egg samples in Iran

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Hassan Bahramianfard ◽  
Abdollah Derakhshandeh ◽  
Zahra Naziri ◽  
Reza Khaltabadi Farahani
Keyword(s):  
Antimicrobial Resistance ◽  
Nalidixic Acid ◽  
Salmonella Enteritidis ◽  
Virulence Genes ◽  
Control Measures ◽  
Poultry Meat ◽  
Diffusion Method ◽  
Virulence Plasmid ◽  
Disk Diffusion Method ◽  
Zoonotic Risk

Abstract Background Salmonella enterica serovar Enteritidis (S. Enteritidis) is one of the most common serovars, associated with human salmonellosis. The food-borne outbreak of this bacterium is mainly related to the consumption of contaminated poultry meat and poultry products, including eggs. Therefore, rapid and accurate detection, besides investigation of virulence characteristics and antimicrobial resistance profiles of S. Enteritidis in poultry and poultry egg samples is essential. A total of 3125 samples (2250 poultry and 875 poultry egg samples), sent to the administrative centers of veterinary microbiology laboratories in six provinces of Iran, were examined for Salmonella contamination, according to the ISO 6579 guideline. Next, duplex PCR was conducted on 250 presumptive Salmonella isolates to detect invA gene for identification of the genus Salmonella and sdf gene for identification of S. Enteritidis. Subsequently, the S. Enteritidis isolates were examined for detection of important virulence genes (pagC, cdtB, msgA, spaN, tolC, lpfC, and spvC) and determination of antibiotic resistance patterns against nalidixic acid, trimethoprim-sulfamethoxazole, cephalothin, ceftazidime, colistin sulfate, and kanamycin by the disk diffusion method. Results Overall, 8.7 and 2.3% of poultry samples and 6.3 and 1.3% of eggs were contaminated with Salmonella species and S. Enteritidis, respectively. The invA and msgA genes (100%) and cdtB gene (6.3%) had the highest and the lowest prevalence rates in S. Enteritidis isolates. The spvC gene, which is mainly located on the Salmonella virulence plasmid, was detected in 50.8% of S. Enteritidis isolates. The S. Enteritidis isolates showed the highest and the lowest resistance to nalidixic acid (87.3%) and ceftazidime (11.1%), respectively. Unfortunately, 27.0% of S. Enteritidis isolates were multidrug-resistant (MDR). Conclusion The rate of contamination with Salmonella in the poultry and egg samples, besides the presence of antimicrobial resistant and MDR Salmonella isolates harboring the virulence genes in these samples, could significantly affect food safety and subsequently, human health. Therefore, continuous monitoring of animal-source foods, enhancement of poultry farm control measures, and limiting the use of antibiotics for prophylactic purposes in food producing animals, are essential for reducing the zoonotic risk of this foodborne pathogen for consumers and also choosing effective antibiotics for the treatment of salmonellosis.

scholarly journals The Role of Aquatic Ecosystems (River Tua, Portugal) as Reservoirs of Multidrug-Resistant Aeromonas spp.

Water ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 698
Author(s):  
Sónia Gomes ◽  
Conceição Fernandes ◽  
Sandra Monteiro ◽  
Edna Cabecinha ◽  
Amílcar Teixeira ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Nalidixic Acid ◽  
Water Samples ◽  
Aquatic Ecosystems ◽  
Anthropogenic Activities ◽  
Aquatic Organisms ◽  
Resistant Bacteria ◽  
Anthropogenic Pressures ◽  
Resistance Rates

The inappropriate use of antibiotics, one of the causes of the high incidence of antimicrobial-resistant bacteria isolated from aquatic ecosystems, represents a risk for aquatic organisms and the welfare of humans. This study aimed to determine the antimicrobial resistance rates among riverine Aeromonas spp., taken as representative of the autochthonous microbiota, to evaluate the level of antibacterial resistance in the Tua River (Douro basin). The prevalence and degree of antibiotic resistance was examined using motile aeromonads as a potential indicator of antimicrobial susceptibility for the aquatic environment. Water samples were collected from the middle sector of the river, which is most impacted area by several anthropogenic pressures. Water samples were plated on an Aeromonas-selective agar, with and without antibiotics. The activity of 19 antibiotics was studied against 30 isolates of Aeromonas spp. using the standard agar dilution susceptibility test. Antibiotic resistance rates were fosfomycin (FOS) 83.33%, nalidixic acid (NA) 60%, cefotaxime (CTX) 40%, gentamicin (CN) 26.67%, tobramycin (TOB) 26.67%, cotrimoxazole (SXT) 26.67%, chloramphenicol (C) 16.67%, and tetracycline (TE) 13.33%. Some of the nalidixic acid-resistant strains were susceptible to fluoroquinolones. Multiple resistance was also observed (83.33%). The environmental ubiquity, the natural susceptibility to antimicrobials and the zoonotic potential of Aeromonas spp. make them optimal candidates for studying antimicrobial resistance (AMR) in aquatic ecosystems. Aquatic environments may provide an ideal setting for the acquisition and dissemination of antibiotic resistance because anthropogenic activities frequently impact them. The potential risk of multi- and pan-resistant bacteria transmission between animals and humans should be considered in a “One Health—One World” concept.

scholarly journals Virulence Gene Profile, Antimicrobial Resistance and Multilocus Sequence Typing of Salmonella enterica Subsp. enterica Serovar Enteritidis from Chickens and Chicken Products

Animals ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 97
Author(s):  
Zunita Zakaria ◽  
Latiffah Hassan ◽  
Zawiyah Sharif ◽  
Norazah Ahmad ◽  
Rohaya Mohd Ali ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Multilocus Sequence Typing ◽  
Salmonella Enteritidis ◽  
Molecular Subtypes ◽  
Actin Binding ◽  
Diffusion Method ◽  
Genetic Characteristics ◽  
Meat Samples ◽  
Sequence Types

This study was undertaken to determine the virulence, antimicrobial resistance and molecular subtypes of Salmonella in the Central Region of Peninsular Malaysia. A total of 45 Salmonella Enteritidis were detected from live chicken (cloacal swab), and chicken products (fresh and ready-to-eat meat) samples upon cultural isolation and serotyping. Similarly, an antimicrobial susceptibility test based on the Kirby Bauer disk diffusion method as well as antimicrobial resistance AMR genes, virulence determinants and multilocus sequence typing (MLST) typing were conducted after the Whole Genome Sequencing and analysis of the isolates. The results indicate that sequence types ST1925 (63.7%), and ST11 (26.5%) were the predominant out of the seven sequence types identified (ST292, ST329, ST365, ST423 and ST2132). The phenotypic antimicrobial profile corresponds to the genotypic characterization in that the majority of the isolates that exhibited tetracycline, gentamycin and aminoglycoside resistance; they also possessed the tetC and blaTEM β-Lactam resistance genes. However, isolates from cloacal swabs showed the highest number of resistance genes compared to the chicken products (fresh and ready-to-eat meat) samples. Furthermore, most of the virulence genes were found to cluster in the Salmonella pathogenicity island (SPI). In this study, all the isolates were found to possess SPI-1, which codes for the type III secretion system, which functions as actin-binding proteins (SptP and SopE). The virulence plasmid (VP) genes (spvB, spvC) were present in all genotypes except ST365. The findings of this study, particularly with regard to the molecular subtypes and AMR profiles of the Salmonella Enteritidis serotype shows multidrug-resistance features as well as genetic characteristics indicative of high pathogenicity.

scholarly journals Antimicrobial Susceptibility Pattern of Gram-Negative Bacterial Isolates from Raw Chicken Meat Samples

2019 ◽  
Vol 6 ◽  
pp. 89-95
Author(s):  
Neha Gautam ◽  
Rojan Poudel ◽  
Binod Lekhak ◽  
Milan Kumar Upreti
Keyword(s):  
Antimicrobial Resistance ◽  
Pathogenic Bacteria ◽  
Chicken Meat ◽  
Food Microbiology ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Kathmandu Valley ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Meat Samples

Objectives: This research aims to study the microbial quality of chicken meat available in retail shop of Kathmandu Valley. Methods:  This Study was conducted from June to December 2018 in three different districts of Kathmandu Valley. Samples were collected in sterile plastic bags, labeled properly and stored in an icebox and transported to the Food Microbiology laboratory of Golden Gate International College.  During sample preparation, 25 grams of each sample was taken and transferred to sterile flasks containing 225 ml of buffered peptone water. Potential pathogenic Gram-negative bacteria were isolated by using respective selective media and identified by biochemical test. Antibiotic susceptibility profile of isolates was carried out by Kirby-Bauer disc diffusion method according to CLSI 2017 guideline. Results: Of total 81 chicken meat samples processed; 201 Gram negative bacteria were isolated.  E. coli (100%) was the dominant Gram-negative isolates, followed by Citrobacter spp (62.96%), Pseudomonas spp (40.74%), Proteus spp (19.75%), Salmonella spp (16.04%) and Klebsiella spp (8.64%) respectively. No any multidrug isolates were detected. Conclusion: The study showed that the raw chicken meat samples of Kathmandu valley was highly contaminated with Gram negative potential pathogenic bacteria. Antimicrobial resistance pattern shown by the isolates may indicates that there is not overuse of drug in animals and the less chance of risk of increasing antimicrobial resistance.

scholarly journals Risk factors for antibiotic resistance in Campylobacter spp. isolated from raw poultry meat in Switzerland

2003 ◽  
Vol 3 (1) ◽  
Author(s):  
Ursula Ledergerber ◽  
Gertraud Regula ◽  
Roger Stephan ◽  
Jürg Danuser ◽  
Béatrice Bissig ◽  
...  
Keyword(s):  
Risk Factors ◽  
Antibiotic Resistance ◽  
Poultry Meat ◽  
Raw Poultry ◽  
Campylobacter Spp

scholarly journals Antimicrobial resistance in Salmonella enterica serovar Enteritidis in Morocco

2010 ◽  
Vol 4 (12) ◽  
pp. 804-809 ◽  
Author(s):  
Farida Ohmani ◽  
Khadija Khedid ◽  
Saad Britel ◽  
Aicha Qasmaoui ◽  
Reda Charof ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Nalidixic Acid ◽  
Salmonella Enterica ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Gastrointestinal Infections ◽  
Disk Diffusion Method ◽  
Resistance Patterns ◽  
Resistance Rates

Introduction: Salmonella enterica is recognised worldwide as one of the major agents of human gastrointestinal infections. The aim of the present work is to ascertain the antimicrobial susceptibilities of 150 Salmonella enterica serovar Enteritidis isolates from humans in Morocco during the period from 2000 to 2008. Methodology: Antimicrobial resistance determination was performed by disk diffusion method using seven antibiotics. The minimal inhibitory concentration (MIC) of ciprofloxacin was determined for nalidixic acid-resistant (NAR) isolates using E-test strips. Results: Sixty-one (42%) isolates were resistant to at least one class of antimicrobial agent. The largest numbers of resistant isolates were observed for nalidixic acid with 53 isolates (36%) followed by ampicillin with 7 isolates (5%), tetracycline with 6 isolates (4%), and trimethoprim/sulfamethoxazole with 2 isolates (1%).The resistant isolates were grouped in seven different resistance patterns of which two isolates were resistant to three antibiotics. Among the 53 (36%) NAR isolates, 37 (76%) had a reduced susceptibility to ciprofloxacin. Conclusion: Resistance rates of Salmonella enterica serovar Enteritidis from Morocco are generally low but the resistance to nalidixic acid is worryingly common. Continual surveillance of antibiotic resistance is of primary importance.

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