scholarly journals Uji Potensi Ekstrak Daun Pepaya (Carica papaya linn) sebagai Larvasida terhadap Larva Aedes sp. Di Manado

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Nazzirah A. Ammari ◽  
Greta J. P. Wahongan ◽  
Janno B. B. Bernadus

Abstract: Papaya leaf extract as a natural larvicide can be used as an alternative to control resistant Aedes aegypti populations. The benefits of controlling the dengue hemorrhagic fever (DHF) vektor are reducing the level of morbidity, mortality and suffering of individuals and their families. This research was conducted with a simple laboratory experimental method. Used papaya leaves extract and larvae of Aedes sp. instar III and IV taken in water float. This study used 100 larvae divided into 2 groups with positive groups, namely the concentration of 5gr, 10 gr, 15 gr and 20 gr and a control group that only used aquadesh which was repeated twice. This study aimed to determine the potential of papaya leaf extract (Carica Papaya Linn) as a larvicide against larvae of Aedes sp. in Manado. In conclusion, papaya leaf extract (Carica Papaya Linn) has potential as a larvicide against Aedes sp. Larvae. where the higher the extract concentration given, so the mortality rate of Aedes sp. larvae higher.Keyword :  Papaya Leaves (Carica Papaya Linn), Larvacides, Larvae Aedes sp.  Abstrak: Ekstrak daun pepaya sebagai larvasida alami dapat digunakan sebagai salah satu alternatif untuk mengendalikan populasi Aedes aegypti yang telah resisten. Manfaat dari pengendalian vektor penyakit demam berdarah dengue (DBD) adalah mengurangi tingkat kesakitan, kematian, dan penderitaan individu beserta keluarganya. Tujuan penelitian ini untuk mengetahui potensi ekstrak daun papaya (Carica Papaya Linn) sebagai larvasida terhadap larva Aedes sp. di Manado. Penelitian ini dilakukan dengan  metode eksperimental laboratoris sederhana.  Menggunakan ekstrak daun pepaya dan larva Aedes sp. instar III dan IV yang di ambil di penampungan air. Penelitian ini menggunakan 100 ekor larva yang terbagi atas 2 kelompok dengan kelompok uji yaitu konsentrasi 5gr, 10 gr, 15 gr dan 20 gr serta kelompok control yang hanya menggunakan aquadesh  yang diulangi sebanyak dua kali percobaan. Hasil nilai p yang didapat dari uji Kruskall Wallis memiliki nilai p < 0.05, hal ini menunjukkan bahwa terdapat perbedaan tingkat kematian larva Aedes sp. setelah 6 jam, 12 jam, 18 jam dan 24 jam pada berbagai konsentrasi perlakuan. Sebagai simpulan, ekstrak daun papaya (Carica Papaya Linn) memiliki potensi sebagai  larvasida terhadap larva Aedes sp. dimana semakin tinggi konsentrasi ekstrak yang diberikan maka semakin tinggi pula tingkat kematian larva Aedes sp. Kata Kunci : Daun Pepaya (Carica Papaya Linn), Larvasida, Larva Aedes sp.

2021 ◽  
Vol 913 (1) ◽  
pp. 012110
Author(s):  
N F Dhenge ◽  
P Pakan ◽  
K Lidia

Abstract Dengue Hemorrhagic Fever (DHF) is an infection caused by the dengue virus, which is still a public health problem in Indonesia. Chemical insecticides as larvicides used to control Aedes aegypti have created resistant populations. Thus, higher doses are needed, which, of course, have toxic effects on animals, humans, and the environment.To analyze the effectiveness of papaya leaf extract (Carica papaya) against the mortality of the third instar Aedesaegypti mosquito larvae. This study is a true experimental research design with a posttest-only control group design. In this study, the groups in this study were the larvae of the Aedes aegypti mosquito instar III/IV, papaya leaf ethanol extract, abate as a positive control group, and aquadest as a negative control.The results of the mean number of larvae deaths in each treatment group was zero (0) larvae in negative control group, positive control group (25 larvae), 5% concentration (9.5 larvae), 10% concentration (11.75 larvae), 15% concentration (12.75 larvae), a concentration of 20% (14.75 larvae), and a concentration of 25% (19.5 larvae). The results of Kruskal Wallis analysis showed that papaya leaf extract was effective as a larvicide for Aedesaegypti (p=0.001). In the probit analysis, it was found that the LC50 of the extract against Aedes aegypti was 23%, while the LC99 was 55%.Papaya leaf extract is effective as a vegetable larvicide for 3rd/IV instar Aedesaegypti larvae


2018 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Khoiriyanti Wulandari ◽  
Mei Ahyanti

<p>Dengue Hemorrhagic Fever (DHF) or Dengue Hemorrhagic Fever (DHF) is a disease caused by a virus which is very dangerous because it can cause the patient to die within a few days. Bandar Lampung city itself is a dengue endemic area. According to data from the Lampung Provincial Health Office, in 2015, from 15 there were 2,996 deaths in 31 people. The study aimed to knowing the effectiveness of bintaro seed extract (Cerbera manghas) as larvacide in III instars Aedes aegypti larvacides experimental research with post test only control group design. The sampling method was purposive sampling. Independent variables were bintaro seed extract (dose and time), the dependent variable was Aedes aegypti larvAedes The results showed that from 5 types of doses with 5 repetitions, the death result of Aedes aegypti larvae with a dose of 0% had no mortality, a dose of 0.1% with an average of 12.55, a dose of 0.5% with an average of 15.15 , 1% dose with an average of 18.20, a dose of 1.5% with an average of 22.15, and a dose of 2% with an average of 25. At the time of contact, the death result of the larvae was found with an average 6-hour contact time. an average of 8.70, 12 hours with an average of 14.13, 24 hours with an average of 18.37 and 48 hours with an average of 20.83. According to the results of statistical tests, the most effective effect of bintaro seed extract dosage is at a dose of 1.5% and contact time of 6 hours.<strong></strong></p>


2020 ◽  
Vol 20 (3) ◽  
Author(s):  
Cut Meurah Yeni ◽  
Dara Meutia Ayu

Abstrack. Demam berdarah dengue (DBD) adalah infeksi virus ditemukan pada negara-negara tropistermasuk Indonesia. Indonesia termasuk dalam katagori A dalam perkembangan penyakit virus tersebut yang mengindikasikan tingginya angka kematian. Angka insiden terus meningkat pada anak maupun dewasa dengan tingginya angka mortalitas. Tidak ada pencatatan lengkap untuk angka insidensi pada kehamilan, meskipun mortalitas cukup tinggi. Kami melaporkan empat kasus kehamilan dengan penyakit yang disebabkan oleh vektor Aedes aegypti. Pada keempat kasus tersebut kami telah diagnosis berdasarkan kriteria demam yang khas, trombositopenia, hemokonsentrasi dan pemeriksaan serologi. Terdapat penatalaksanaan yang berbeda dari berbagai kasus tersebut termasuk indikasi terminasi. Satu pasien kami rawat konservatif dan tiga pasien dilakukan terminasi berdasarkan indikasi obstetri. Pertimbangan cara terminasi sangat bergantung pula dengan indikasi obstetri. Seksio sesarea tidak secara mutlak dilakukan pada kehamilan dengan demam berdarah dengue. Kata kunci: Demam berdarah dengue, kehamilan, penatalaksanaan Abstract. Dengue hemorrhagic fever (DHF) is a viral infection found in tropical countries including Indonesia. Indonesia is included in category A in the development of viral diseases that's the high mortality rate. The numbers keep increasing on both adult children with high mortality rates. No complete logging for the incidence rate in pregnancy, although mortality is quite high. We reported four cases pregnancy with diseases caused by the Aedes aegypti vector. In four cases We had diagnosed based on criteria that met the specific criteria, thrombocytopenia, hemoconcentration and serology. There are different treatments for these cases includes the term indication. One patient was treated conservatively and three patients were admitted termination based on obstetric indications. The consideration on how to terminate is also very dependent with obstetric indications. Caesarean section is not absolutely necessary in pregnancy with dengue hemorrhagic fever. Keywords: Dengue hemorrhagic fever, pregnancy, management


Biospecies ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 1-7
Author(s):  
Firza Khairullah Sembiring ◽  
Riyani Susan BT Hasan

This research was conducted to determine the effectiveness of betel leaf extract conducted at a concentration of 2.5%, 5.0%, 7.5%, 10% as larvicide toward the development of Aedes Aegypti Sp. Aedes Aegypti Sp larvae are dangerous vectors in Dengue Hemorrhagic Fever (DHF) in humans. The method in this research was carried out on an experimental method by using a post-test only design and sampling with a purposive sampling method. The effectiveness of betel leaf extract larvicide on the development of Aedes Aegypti Sp larvae was carried out by mixing betel leaf extract with several concentrations in one container Aedes Aegypti Sp larvae inside. The results obtained in this research were carried out by the Kruskal-Wallis test, the test found that there was a significant difference because it was given a 95% confidence index. The larvae treated with betel leaf extract with a concentration of 10% had the highest effectiveness in stopping the development of Aedes Aegypti Sp larvae, which proved at the mortality at a concentration of 10%, found total mortality of 20 larvae in 8 hours of treatment. Betel leaf extract with a concentration of 10% had the highest effectiveness of discontinuing the development of Aedes Aegypti Sp larvae.


2019 ◽  
Vol 7 (2) ◽  
pp. 59
Author(s):  
Restu Prastiwi ◽  
Anita Dewi Moelyaningrum ◽  
Prehatin T. N

<p><em>Aedes aegypti mosquito is a type of mosquito that can carry dengue virus that causes dengue fever. This mosquito has the potential to transmit dengue hemorrhagic fever (DHF). DHF is a disease characterized by sudden fever, bleeding both on the skin and in other parts of the body and can cause shock and death. So in order to reduce the impact of the disease, there are several ways that must be taken. One way of controlling that is done to reduce the population of aedes aegypti mosquitoes is by reducing larval growth by using a solution of papaya leaves as larvacide. Papaya leaves contain tannins of 0.12%. So it is possible to kill larvae. The purpose of this study was to analyze the differences in the average mortality of untreated instar 1 aedes aegypti larvae and treated with a solution of distilled papaya leaves, amounting to 0ml / 100ml (X0), 1ml / 100ml (X1), 2ml / 100ml (2) , 4ml / 100m (X3) with observations every 6 hours for 24 hours. This study is a true experimental study with the presentation of the data analyzed using the kruskal wallis test on spss. The results showed that there were differences between the control group and the treatment group (p &lt;0.05). The most significant difference occurred in the 4ml / 100ml (X3) group which had the highest mortality than the other groups. So the higher the concentration and the longer the duration of exposure, the higher the mortality rate in the larvae. </em></p>


Author(s):  
Abdul Khair ◽  
Noraida Noraida

Vector-borne diseases are still a health problem in some tropical countries. One vector-borne disease is Dengue Hemorrhagic Fever (DHF). DHF never decreases and even tends to continue to increase, and many cause deaths in children, 90% of them attack children under 15 years. DHF cases in 2018 amounted to 65,602 cases, with 467 deaths (CFR = 0.71%). The behavior of holding water in various places such as tubs and tendons indirectly creates a breeding place for Aedes aegypti mosquitoes. The use of chemicals as insecticides can cause the death of non-target animals, environmental pollution, and the occurrence of vector resistance to insecticides. Therefore it is necessary to do other methods including the use of vegetable insecticides. Sugar apple leaves can be used to kill Aedes aegypti larvae. Sugar apple leaves will obtain throughout the year. The purpose of this study is to know the ability of young and old sugar apple leaves to kill Aedes aegypti larvae. This type of research is a posttest only control group design. The study sample was part of the Aedes aegypti larvae on the final instar III. The experiment to kill Aedes aegypti larvae use two types of sugar apple leaf extract, namely young and old sugar apple leaves with nine treatments four replications. Each treatment consisted of 25 larvae. The research results show There was no significant difference in the number of dead larvae using either extracts from young sugar apple leaves or old sugar apple leaves. LC90 of sugar apple leaf extract was between 0.05632 to 0.08324% and the effective residual age at LC90 (0.06568%) with the death of Aedes aegypti larvae was 92% over 24 hours (1 day).


2018 ◽  
Vol 2 (1) ◽  
pp. 36
Author(s):  
Setyo Dwi Santoso ◽  
Abdul Chamid ◽  
Dwi Viddi Kusuma Pratiwi

Dengue hemorrhagic fever (DHF) is a very dangerous disease, caused by Aedes aegypti mosquitoes. One alternative to controlling Aedes aegypti mosquitoes is to use insecticides. But it would be much better if the insecticides used are natural and environmentally friendly, one of them by using Tomato Leaf Extract (Solanum lycopersicum l.). Tomato leaves contain chemical compounds class Alkaloids and Saponins and can serve as anti larvacide. This research is a laboratory experiment which aims to find out the effect of tomato leaf extract (Solanum lycopersicum L.) activity on the death of III instar larvae of Aedes aegypti mosquito. the effectiveness of samples on Aedes aegypti mosquito larvae by treatment of variation of extract concentration 0.2%, 0.4%, 0.6%, 0.8%, 1% with repetition 2 times. In the results of research showed that the smallest concentration that can kill Aedes aegypti mosquito larvae is 0.6% at 16 hours and probit analysis showed that significance is 0,608 greater than intercept 0.003, it shows that the extract of tomato leaves affects the death of III instar larvae of Aedes aegypti mosquito.


2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Nurlailah Nurlailah ◽  
Anny Thuraidah

Dengue hemorrhagic fever (DHF) is one of the diseases caused by Aedes Aegypti mosquitoes. DHF is still a public health problem, including in Indonesia, due to the high mortality rate caused by this mosquito. The risk caused by the bite of the Aedes sp mosquito is quite dangerous, including acute fever, bleeding, shock, and even death, so that efforts are needed to control it. One of the natural ingredients that can use to eradicate Aedes Aegypti mosquitoes without causing negative impacts on the environment is the Cananga flower and Zyzigium Leaves. This study purposes of determining the optimum concentration of Cananga Flowers and Zyzigium Leaves Ethanol extract of which was having biolarvaside effect to Aedes Aegypti larvae. The method used a simple experiment with Posttest Only With Control Group Design. The data result of the research analyzed with Logit and Wilcoxon test. Achievement of those tests was LC50 Zyzigium leaf extract on contact for 24 hours was 0.609-0.935, and LC99 was between 1.064-1.973 while Cananga flower extract obtained LC50 of 0.228-0.261 and the LC99 was 0.446 to 0.558. Wilcoxon test for compared bioalarvaside effect results between Cananga Flower and Zyzigium Leaf received a significance value less than α (0.001<0.05), so it can ascertain that the Cananga Flower extract was stronger biolarvaside than Zyzigium Leaf extract.


2021 ◽  
Vol 20 (1) ◽  
pp. 18-25
Author(s):  
Jennifer Wiranatha ◽  
Robby Makimian ◽  
Rita Dewi

Introduction: Dengue hemorrhagic fever is one of the most common infectious diseases in Indonesia. The vector of this disease is Aedes aegypti (Ae. aegypti). Dengue hemorrhagic fever can be controlled by a few measures, one of them is using insecticide. However, frequent use of chemical insecticide could lead to resistance and is harmful to non-target organisms. One of the solutions for this problem is the use of bioinsecticide derived from lemongrass (Cymbopogon citratus or C. citratus) leaves extract. Methods: The design of this study is true experimental post-test only control group. The population used are adult Ae. aegypti mosquitoes. For each experiment, 10 mosquitoes are required and given the extract of C. citratus in 2%, 10%, and 20% concentrations, and negative control respectively.The lethal effect of the extract is observed in 10, 30, 60 minutes and 6, 12, 18, 24 hours. Results: There is a significant difference in the lethal effect of Ae. aegypti mosquitoes with variations in duration of exposure to the C. citratus leaves extract (p = 0.007), but no significant difference with variations in extract concentration given (p = 0.281). Conclusion: C. citratus leaves extract has bioinsecticidal effect on Ae. aegypti mosquitoes. The optimal result of mosquito mortality is achieved by using the extract in 20% concentration with duration of 12 hours.


Author(s):  
Wihdatul Karima ◽  
Syahrul Ardiansyah

Indonesia has a potential to be exposed to the threat of dengue hemorrhagic fever with the point vector, the Aedes aegypti mosquito, so development need to be stopped. The use of synthetic larvicides in eradicating mosquito larvae can cause environmental pollution. To reduce it, an alternative is needed using plant larvacides with banana leaf. Banana leaves are obtained from Candi Sidoarjo. Ethanol extract of banana leaf contains tannin compounds, alkaloids, terpenoids, saponins, and flavonoids which can be used as larvicides. This research was conducted to determine the toxic effects of banana leaf extract (Musa paradisiaca L.) on the larvae of Aedes aegypti mosquito mortality. This research was conducted using the post test only the control group design with 6 treatment groups including control (aquades) and banana leaf  extract concentrations of 1000 ppm, 2000 ppm, 3000 ppm, 4000 ppm, 5000 ppm. This study used third instar larvae, each test group countaining 20 larvae with 4 repetitions. That obtained were analyzed using data and probit tests. The results of this study that banana leaf extract has a toxic effect on Aedes aegypti mosquito larvae with LC50 at a concentration of 4638 ppm.


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