scholarly journals Lethal Efficacy of Banana Leaves Extract (Musa paradisiaca L.) Against Aedes aegypti Larvae

2021 ◽  
Vol 4 (1) ◽  
pp. 7-12
Author(s):  
Wihdatul Karima ◽  
Syahrul Ardiansyah
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Test Group ◽  
Hemorrhagic Fever ◽  
Control Group ◽  
Mosquito Larvae ◽  
Control Group Design ◽  
Musa Paradisiaca ◽  
Banana Leaves ◽  
Banana Leaf

Indonesia has a potential to be exposed to the threat of dengue hemorrhagic fever with the point vector, the Aedes aegypti mosquito, so development need to be stopped. The use of synthetic larvicides in eradicating mosquito larvae can cause environmental pollution. To reduce it, an alternative is needed using plant larvacides with banana leaf. Banana leaves are obtained from Candi Sidoarjo. Ethanol extract of banana leaf contains tannin compounds, alkaloids, terpenoids, saponins, and flavonoids which can be used as larvicides. This research was conducted to determine the toxic effects of banana leaf extract (Musa paradisiaca L.) on the larvae of Aedes aegypti mosquito mortality. This research was conducted using the post test only the control group design with 6 treatment groups including control (aquades) and banana leaf  extract concentrations of 1000 ppm, 2000 ppm, 3000 ppm, 4000 ppm, 5000 ppm. This study used third instar larvae, each test group countaining 20 larvae with 4 repetitions. That obtained were analyzed using data and probit tests. The results of this study that banana leaf extract has a toxic effect on Aedes aegypti mosquito larvae with LC50 at a concentration of 4638 ppm.

scholarly journals Young and Old Sugar Apple (Annona squamosa Linn) Leaf Extracts As an Aedes aegypti Larva Insecticide

2019 ◽  
Author(s):  
Abdul Khair ◽  
Noraida Noraida
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Hemorrhagic Fever ◽  
Control Group ◽  
Leaf Extracts ◽  
Control Group Design ◽  
Vector Borne Diseases ◽  
Apple Leaves ◽  
Significant Difference ◽  
Vector Borne

Vector-borne diseases are still a health problem in some tropical countries. One vector-borne disease is Dengue Hemorrhagic Fever (DHF). DHF never decreases and even tends to continue to increase, and many cause deaths in children, 90% of them attack children under 15 years. DHF cases in 2018 amounted to 65,602 cases, with 467 deaths (CFR = 0.71%). The behavior of holding water in various places such as tubs and tendons indirectly creates a breeding place for Aedes aegypti mosquitoes. The use of chemicals as insecticides can cause the death of non-target animals, environmental pollution, and the occurrence of vector resistance to insecticides. Therefore it is necessary to do other methods including the use of vegetable insecticides. Sugar apple leaves can be used to kill Aedes aegypti larvae. Sugar apple leaves will obtain throughout the year. The purpose of this study is to know the ability of young and old sugar apple leaves to kill Aedes aegypti larvae. This type of research is a posttest only control group design. The study sample was part of the Aedes aegypti larvae on the final instar III. The experiment to kill Aedes aegypti larvae use two types of sugar apple leaf extract, namely young and old sugar apple leaves with nine treatments four replications. Each treatment consisted of 25 larvae. The research results show There was no significant difference in the number of dead larvae using either extracts from young sugar apple leaves or old sugar apple leaves. LC90 of sugar apple leaf extract was between 0.05632 to 0.08324% and the effective residual age at LC90 (0.06568%) with the death of Aedes aegypti larvae was 92% over 24 hours (1 day).

scholarly journals Biolarvasides of Cananga odorata Flower and Zyzygium polyanthum Leaves Ethanol Extract on Aedes aegypti Larva

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Nurlailah Nurlailah ◽  
Anny Thuraidah
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Public Health Problem ◽  
Ethanol Extract ◽  
Hemorrhagic Fever ◽  
Wilcoxon Test ◽  
Control Group ◽  
Control Group Design ◽  
Flower Extract ◽  
Negative Impacts

Dengue hemorrhagic fever (DHF) is one of the diseases caused by Aedes Aegypti mosquitoes. DHF is still a public health problem, including in Indonesia, due to the high mortality rate caused by this mosquito. The risk caused by the bite of the Aedes sp mosquito is quite dangerous, including acute fever, bleeding, shock, and even death, so that efforts are needed to control it. One of the natural ingredients that can use to eradicate Aedes Aegypti mosquitoes without causing negative impacts on the environment is the Cananga flower and Zyzigium Leaves. This study purposes of determining the optimum concentration of Cananga Flowers and Zyzigium Leaves Ethanol extract of which was having biolarvaside effect to Aedes Aegypti larvae. The method used a simple experiment with Posttest Only With Control Group Design. The data result of the research analyzed with Logit and Wilcoxon test. Achievement of those tests was LC50 Zyzigium leaf extract on contact for 24 hours was 0.609-0.935, and LC99 was between 1.064-1.973 while Cananga flower extract obtained LC50 of 0.228-0.261 and the LC99 was 0.446 to 0.558. Wilcoxon test for compared bioalarvaside effect results between Cananga Flower and Zyzigium Leaf received a significance value less than α (0.001<0.05), so it can ascertain that the Cananga Flower extract was stronger biolarvaside than Zyzigium Leaf extract.

scholarly journals Larvacide effectiveness of Papaya leaf extract (Carica papaya) on the mortality of larvae vector of Dengue hemorrhagic fever caused by Aedes aegypti

2021 ◽  
Vol 913 (1) ◽  
pp. 012110
Author(s):  
N F Dhenge ◽  
P Pakan ◽  
K Lidia
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design

Abstract Dengue Hemorrhagic Fever (DHF) is an infection caused by the dengue virus, which is still a public health problem in Indonesia. Chemical insecticides as larvicides used to control Aedes aegypti have created resistant populations. Thus, higher doses are needed, which, of course, have toxic effects on animals, humans, and the environment.To analyze the effectiveness of papaya leaf extract (Carica papaya) against the mortality of the third instar Aedesaegypti mosquito larvae. This study is a true experimental research design with a posttest-only control group design. In this study, the groups in this study were the larvae of the Aedes aegypti mosquito instar III/IV, papaya leaf ethanol extract, abate as a positive control group, and aquadest as a negative control.The results of the mean number of larvae deaths in each treatment group was zero (0) larvae in negative control group, positive control group (25 larvae), 5% concentration (9.5 larvae), 10% concentration (11.75 larvae), 15% concentration (12.75 larvae), a concentration of 20% (14.75 larvae), and a concentration of 25% (19.5 larvae). The results of Kruskal Wallis analysis showed that papaya leaf extract was effective as a larvicide for Aedesaegypti (p=0.001). In the probit analysis, it was found that the LC50 of the extract against Aedes aegypti was 23%, while the LC99 was 55%.Papaya leaf extract is effective as a vegetable larvicide for 3rd/IV instar Aedesaegypti larvae

scholarly journals Efektivitas Ekstrak Biji Bintaro (Cerbera manghas) sebagai Larvasida Hayati pada Larva Aedes aegypti Instar III

2018 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Khoiriyanti Wulandari ◽  
Mei Ahyanti
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Contact Time ◽  
Statistical Tests ◽  
Hemorrhagic Fever ◽  
Seed Extract ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Cerbera Manghas

<p>Dengue Hemorrhagic Fever (DHF) or Dengue Hemorrhagic Fever (DHF) is a disease caused by a virus which is very dangerous because it can cause the patient to die within a few days. Bandar Lampung city itself is a dengue endemic area. According to data from the Lampung Provincial Health Office, in 2015, from 15 there were 2,996 deaths in 31 people. The study aimed to knowing the effectiveness of bintaro seed extract (Cerbera manghas) as larvacide in III instars Aedes aegypti larvacides experimental research with post test only control group design. The sampling method was purposive sampling. Independent variables were bintaro seed extract (dose and time), the dependent variable was Aedes aegypti larvAedes The results showed that from 5 types of doses with 5 repetitions, the death result of Aedes aegypti larvae with a dose of 0% had no mortality, a dose of 0.1% with an average of 12.55, a dose of 0.5% with an average of 15.15 , 1% dose with an average of 18.20, a dose of 1.5% with an average of 22.15, and a dose of 2% with an average of 25. At the time of contact, the death result of the larvae was found with an average 6-hour contact time. an average of 8.70, 12 hours with an average of 14.13, 24 hours with an average of 18.37 and 48 hours with an average of 20.83. According to the results of statistical tests, the most effective effect of bintaro seed extract dosage is at a dose of 1.5% and contact time of 6 hours.<strong></strong></p>

scholarly journals Efektivitas Ekstrak Daun Sirih (Piper betle L) dan Ekstrak Bawang Putih (Allium sativum) terhadap Mortalitas Larva Nyamuk Aedes aegypti

bionature ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Amirullah Amirullah ◽  
Nurhayu Malik ◽  
Rosmaya Rosmaya
Keyword(s):  
Aedes Aegypti ◽  
Endemic Area ◽  
Leaf Extract ◽  
Allium Sativum ◽  
Larval Mortality ◽  
Hemorrhagic Fever ◽  
Garlic Extract ◽  
Piper Betle ◽  
Mosquito Larvae ◽  
Betel Leaf

Abstract. The study of the efficacy of betel leaf extract (Piper betle L.) and garlic extract (Allium sativum) on the mortality of Aedes aegypti mosquito larvae was carried out at the Zoology Laboratory of the Faculty of Mathematics and Natural Sciences, Halu Oleo Kendari University. Aedes aegypti mosquito larvae were obtained from the rearing of F2 from Aedes aegypti eggs that were taken by ovitrap from in Kambu Village, Kendari City, which is an endemic area of Dengue Hemorrhagic Fever (DHF). Extrac of betel leaf (Piper betle L.) and garlic extract (Allium sativum) at concentrations of 1.0%, 2.0% and 3.0% respectively and their combinations were given to Aedes aegypti larvae (L4). The results showed that the highest larval mortality (88%) occurred after 24 hours of exposure to betel leaf extract (Piper betle L.) at a concentration of 3%, whereas with garlic extract (Allium sativum) the highest larval mortality (98%) occurred at concentration 3 %, and for the highest larval mortality (99%) found in a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum). Based on WHO (2009), the most effective treatment for killing Aedes aegyti larvae is 3% garlic (Allium sativum) extract and a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum).Keywords:  Biopesticide, Mortality, Aedes aegypti, betle leaf (Piper betle L.), garlic (Allium sativum).

scholarly journals The Effect of Sugar Apple (Annona squamosa L.) Seeds Solution as an Insecticide Against Mortality of Aedes aegypti Mosquito with Liquid Electric Method

2018 ◽  
Vol 9 (2) ◽  
pp. 200
Author(s):  
Amalinda Mega Novasari ◽  
Retno Sasongkowati
Keyword(s):  
Aedes Aegypti ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Cross Sectional Study ◽  
Primary Data ◽  
Control Group ◽  
Annona Squamosa ◽  
Control Group Design ◽  
Cross Sectional ◽  
Apple Seeds

Dengue hemorrhagic fever is a public health problem in Indonesia that the number of DHF cases has been increased. Morbidity number of DHF per 100,000 population at 2011 was 26.67 became 34.3 in 2012. The control of DHF has been attempted, but a lot of synthetic insecticide used has been negatively affected to environment. So, it need the insecticide alternative. The aim of study was to identify the effect of solvent sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method. Type of this research was experimental with cross sectional study design as well as the post test only control group design. The study was conducted from February to July 2013 in the Laboratory of Entomology of the Provincial Health Office of East Java. The population of this research was the Aedes aegypti with 560 sample of mosquitoes were divided into five treatments and four repetitions for each treatment. The collection of quantitative data drawn from primary data through observation by calculating the death of Aedes aegypti of each the sugar apple seeds concentration of 10%, 20%, 30%, 40% and 50% for 24 hours. The results showed that the concentration of 50% solvent of sugar apple seeds provide the greatest effect in causing the death of Aedes aegypti mosquitoes as many as 36 died after exposure. It can be concluded that there are effected by giving solvent of sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method.

scholarly journals EFEKTIVITAS ABATE DAN EKSTRAK DAUN SIRIH (Piper betle) DALAM MEMATIKAN LARVA Aedes Aegypti L INSTAR III

Masker Medika ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 226-232
Author(s):  
Triana Oktaviani ◽  
Zairinayati Zairinayati
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Hemorrhagic Fever ◽  
Piper Betle ◽  
Mosquito Larvae ◽  
Betel Leaf ◽  
Preventive Effort ◽  
Randomized Design ◽  
Natural Insecticides ◽  
Completely Randomized Design

Latar Belakang: Aedes aegypti merupakan nyamuk yang dapat berperan sebagai vektor Demam Berdarah Dengue (DBD). Upaya promotif dan preventif menjadi mutlak dilakukan untuk memutus mata rantai penularan dengan membunuh larva nyamuk dengan larvasida alami menggunakan ekstrak daun sirih (Piper betle) sebagai insektisida alami. Tujuan dari penelitian: ini adalah untuk mengetahui perbedaan abate dan ekstrak daun sirih (Piper betle) dalam mematikan larva Aedes aegypti L instar III. Desain penelitian: ini bersifat eksperimen dengan menggunakan rancangan acak lengkap (RAL) yang terdiri dari 3 perlakuan 1 kontrol dan 8 kali pengulangan percobaan. Hasil Penelitian: dapat diketahui bahwa untuk penggunaan ekstrak daun sirih (Piper betle) dengan konsentrasi 5% dapat mematikan larva sebanyak 8 ekor, sedangkan konsentrasi 10% tidak dapat mematikan larva (0 larva) dan konsentrasi 15% dapat mematikan larva sebanyak 17 (85%) sedangkan untuk penggunaan abate dengan konsentrasi 5%, 10% dan 15% dapat mematikan larva sebanyak 160 larva (100%). Kesimpulan: bahwa abate mempunyai efektifitas lebih tinggi di banding dengan ekstrak daun sirh (Piper betle).     Background: Aedes aegypti is a mosquito that can act as a vector of dengue Hemorrhagic Fever (DHF). Promotove and preventive effort are absolutely necessary to break the chain to transmission by killing mosquito larvae with natural larvacides using betel leaf extract (Piper betle) as a natural insecticides. The purposes of this study: this was to determine the differences in abate and betel leaf extract (Piper batle) in kliing Aedes aegypti instar III larvae. Research methods this is an experimental study using a completely randomized design (CRD) consisting of 3 treatments 1 control and 8 repetitions of the experiment. The results: it can be seen that for the use of betel leaf extract(Piper betle) with a concentration cannot kill larvae in 17 (85%) whilke for the use of abate with a concentration of 5%, 10% and 15% can kill larvae of 160 (100%). Conclusion: that the abate has a higher effectiveness compared to sirih leaf extract.

scholarly journals DAYA BUNUH EKSTRAK DAUN TOMAT (Solanum lycopersicum L.) TERHADAP LARVA NYAMUK Aedes aegypti

2018 ◽  
Vol 2 (1) ◽  
pp. 36
Author(s):  
Setyo Dwi Santoso ◽  
Abdul Chamid ◽  
Dwi Viddi Kusuma Pratiwi
Keyword(s):  
Aedes Aegypti ◽  
Solanum Lycopersicum ◽  
Leaf Extract ◽  
Chemical Compounds ◽  
Hemorrhagic Fever ◽  
Probit Analysis ◽  
Tomato Leaf ◽  
Mosquito Larvae ◽  
Extract Concentration ◽  
Solanum Lycopersicum L

Dengue hemorrhagic fever (DHF) is a very dangerous disease, caused by Aedes aegypti mosquitoes. One alternative to controlling Aedes aegypti mosquitoes is to use insecticides. But it would be much better if the insecticides used are natural and environmentally friendly, one of them by using Tomato Leaf Extract (Solanum lycopersicum l.). Tomato leaves contain chemical compounds class Alkaloids and Saponins and can serve as anti larvacide. This research is a laboratory experiment which aims to find out the effect of tomato leaf extract (Solanum lycopersicum L.) activity on the death of III instar larvae of Aedes aegypti mosquito. the effectiveness of samples on Aedes aegypti mosquito larvae by treatment of variation of extract concentration 0.2%, 0.4%, 0.6%, 0.8%, 1% with repetition 2 times. In the results of research showed that the smallest concentration that can kill Aedes aegypti mosquito larvae is 0.6% at 16 hours and probit analysis showed that significance is 0,608 greater than intercept 0.003, it shows that the extract of tomato leaves affects the death of III instar larvae of Aedes aegypti mosquito.

scholarly journals Uji Potensi Ekstrak Daun Pepaya (Carica papaya linn) sebagai Larvasida terhadap Larva Aedes sp. Di Manado

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Nazzirah A. Ammari ◽  
Greta J. P. Wahongan ◽  
Janno B. B. Bernadus
Keyword(s):  
Mortality Rate ◽  
Aedes Aegypti ◽  
Experimental Method ◽  
Dengue Hemorrhagic Fever ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Hemorrhagic Fever ◽  
Control Group ◽  
Extract Concentration ◽  
Positive Groups

Abstract: Papaya leaf extract as a natural larvicide can be used as an alternative to control resistant Aedes aegypti populations. The benefits of controlling the dengue hemorrhagic fever (DHF) vektor are reducing the level of morbidity, mortality and suffering of individuals and their families. This research was conducted with a simple laboratory experimental method. Used papaya leaves extract and larvae of Aedes sp. instar III and IV taken in water float. This study used 100 larvae divided into 2 groups with positive groups, namely the concentration of 5gr, 10 gr, 15 gr and 20 gr and a control group that only used aquadesh which was repeated twice. This study aimed to determine the potential of papaya leaf extract (Carica Papaya Linn) as a larvicide against larvae of Aedes sp. in Manado. In conclusion, papaya leaf extract (Carica Papaya Linn) has potential as a larvicide against Aedes sp. Larvae. where the higher the extract concentration given, so the mortality rate of Aedes sp. larvae higher.Keyword :  Papaya Leaves (Carica Papaya Linn), Larvacides, Larvae Aedes sp.  Abstrak: Ekstrak daun pepaya sebagai larvasida alami dapat digunakan sebagai salah satu alternatif untuk mengendalikan populasi Aedes aegypti yang telah resisten. Manfaat dari pengendalian vektor penyakit demam berdarah dengue (DBD) adalah mengurangi tingkat kesakitan, kematian, dan penderitaan individu beserta keluarganya. Tujuan penelitian ini untuk mengetahui potensi ekstrak daun papaya (Carica Papaya Linn) sebagai larvasida terhadap larva Aedes sp. di Manado. Penelitian ini dilakukan dengan  metode eksperimental laboratoris sederhana.  Menggunakan ekstrak daun pepaya dan larva Aedes sp. instar III dan IV yang di ambil di penampungan air. Penelitian ini menggunakan 100 ekor larva yang terbagi atas 2 kelompok dengan kelompok uji yaitu konsentrasi 5gr, 10 gr, 15 gr dan 20 gr serta kelompok control yang hanya menggunakan aquadesh  yang diulangi sebanyak dua kali percobaan. Hasil nilai p yang didapat dari uji Kruskall Wallis memiliki nilai p < 0.05, hal ini menunjukkan bahwa terdapat perbedaan tingkat kematian larva Aedes sp. setelah 6 jam, 12 jam, 18 jam dan 24 jam pada berbagai konsentrasi perlakuan. Sebagai simpulan, ekstrak daun papaya (Carica Papaya Linn) memiliki potensi sebagai  larvasida terhadap larva Aedes sp. dimana semakin tinggi konsentrasi ekstrak yang diberikan maka semakin tinggi pula tingkat kematian larva Aedes sp. Kata Kunci : Daun Pepaya (Carica Papaya Linn), Larvasida, Larva Aedes sp.

scholarly journals Potensi Minyak Atsiri Bunga Lawang (Illicium verum) sebagai Repelen Nyamuk Aedes aegypti

2019 ◽  
pp. 13-22
Author(s):  
Eva Lestari ◽  
Bondan Fajar Wahyudi ◽  
Adil Ustiawan ◽  
Dian Indra Dewi
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Star Anise ◽  
Post Test ◽  
Illicium Verum

Dengue Hemorrhagic Fever (DHF) is still a become public health problem in the world. In 2017, there were  68,407 cases with 493 deaths DHF cases in Indonesia . The incidence rate of DHF in 2017 was 26,10/100,000 population. DHF was caused by dengue virus which was transmitted through the bites of Aedes aegypti. Several types of plants such as stems, leaves, and flowers can be used as natural ingredients for repelling Ae. aegypti, Star anise (Illicium verum) has a chemical compound that can be used as a mosquito repellent. The purpose of this study was to know the potential of star anise essential oil as repellents of Ae. aegypti. This study was an experimental study with post test only with control group design. The experiment to test  the repellency of star anise essential oil was carried out using concentrations of 10%, 20%, 30%, 40%, and 50%  on 3-5 days old female Ae. aegypti. The result of this study showed that repellency index of star anise essential oil at concentration 10%, 20%, 30%, 40% and 50% during 6 hours were 58.1%; 63.51%; 59.95%; 49.45% and 65.32%. Star anise essential oil at concentration 10%, 20%, 30%, 40%, and 50% can provide protection against the bites of Ae. aegypti for 1 until 2 hours.

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