scholarly journals Identifikasi Senyawa Bioaktif Alga Merah Halymenia durvillei (Identification Bioactive Compounds of Algae Halymenia durvillei)

2019 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
Marina Singkoh ◽  
Desy Mantiri ◽  
Cyska Lumenta ◽  
Hengky Manoppo
Keyword(s):  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Red Algae ◽  
Screening Method ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
North Sulawesi ◽  
Screening Study

Identifikasi Senyawa Bioaktif  Alga Merah Halymenia durvillei(Identification Bioactive Compounds of Algae Halymenia durvillei) Marina Flora Oktavine Singkohˡ)*, Desy Maria Helena  Mantiri ²) Cyska Lumenta²), Henky Manoppo²)1) Program Studi Biologi FMIPA Universitas Sam Ratulangi, Manado 951152) Fakultas Perikanan dan Ilmu Kelautan Universitas Sam Ratulangi, Manado *Email korespondensi: [email protected]  Diterima 17 Februari  2019, diterima untuk dipublikasikan 28 Februari  2019  Abstrak Alga merah memiliki kemampuan untuk memproduksi metabolit sekunder yang bersifat sebagai senyawa bioaktif. Penelitian ini bertujuan untuk mengidentifikasi senyawa bioaktif pada alga merah Halymenia durvillaei. yang diambil dari pesisir Pantai Desa Rendingan, Kecamatan Tabukan Kepulauan Sangihe, Sulawesi Utara dengan metode skrining Fitokimia. Hasil penelitian  menunjukan bahwa alga merah  Halymenia durvillaei mengandung senyawa-senyawa  bioaktif. Berdasarkan hasil penelitian skrining fitokimia dapat disimpulkan bahwa  ekstrak etanol Halymenia durvillaei  mengandung senyawa bioaktif alkaloid, fenol, saponin, tanin, dan steroid.Kata Kunci: Halymenia durvillaei,  senyawa bioaktif,  fitokimia  Abstract Red algae have the ability to produce secondary metabolites that are bioactive compounds. This study aims to identify bioactive compounds in Halymenia durvillaei red algae taken from the coast of Rendingan Village Beach, Tabukan District, Sangihe Islands, North Sulawesi with the phytochemical screening method. The results showed that Halymenia durvillaei red algae contained bioactive alkaloid compounds. Based on the results of the phytochemical screening study it can be concluded that the Halymenia durvillaei ethanol extract contains bioactive alkaloid compounds, phenols, saponins, tannins, and steroids.Keywords: Halymenia durvillaei, bioactive compounds,  phytochemicals

scholarly journals Phytochemical Screening, Spectrum Profile of Functional Groups, and Effervescent Formulation of Kepok Banana Peels Stem Extract

2021 ◽  
Vol 4 (2) ◽  
pp. 66-72
Author(s):  
Robby Candra Purnama ◽  
Annisa Primadiamanti
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Secondary Metabolites ◽  
Functional Groups ◽  
Functional Group ◽  
Screening Method ◽  
Phytochemical Screening ◽  
Stem Extract ◽  
Group Profile ◽  
Banana Stem

Kepok banana plants contain secondary metabolites such as tannins and flavonoids. Tannins and flavonoids have various properties for human health. Research has been carried out to identify secondary metabolite compounds (tannins, flavonoids, and saponins) by using the phytochemical screening method to see the functional group profile contained in the extract of kepok banana stem waste. Kepok banana stem waste was extracted in 96% ethanol, then evaporated and screened phytochemically. This extract was used to prepare effervescently. Screening results showed that tannin and flavonoids were identified by the appearance of the following color black-green and dark red, respectively. Meanwhile, saponins were negative because the foam formed had a height of 0.3 cm and did not meet the saponins' positive requirements (1-3 cm high foam and stable for 5 minutes). Identification of functional groups in the extract of kepok banana stem waste using Fourier-transform Infrared Spectroscopy (FTIR) showed that C-C stretching in the area 2927.24 cm-1, O-H stretching in the 3423.87 cm-1 area, C=O stretching in the 1648.87 cm-1 area. Also appeared bending CH2 in the region of 1421.45 cm-1, and C-C in the area of 1149.98 cm-1. The characteristics of three different formulas (A, B, and C) of effervescent have been investigated: the moisture content of 2.51%; 2.55%, and 2.52%, respectively. Then, flow rate of 8.81 g/s; 8.83 g/s; and 8.82 g/s, compressibility of 14.5%; 14.4%; and 14.5%, and a pH of 5.97; 5.98; and 5.97 respectively. All parameters are eligible.

scholarly journals KEKAYAAN DAN POTENSI SENYAWA BIOAKTIF MAKROALGA DI PESISIR ATEP OKI, KABUPATEN MINAHASA, SULAWESI UTARA

2020 ◽  
Vol 8 (3) ◽  
pp. 7
Author(s):  
Silsia Dorkas Winowoda ◽  
Marina Flora Oktavine Singkoh ◽  
Ratna Siahaan
Keyword(s):  
Green Algae ◽  
Bioactive Compounds ◽  
Red Algae ◽  
Brown Algae ◽  
North Sulawesi ◽  
Sampling Locations

This study aims to analyze the richness and potential of macroalgae bioactive compounds on the Atep Oki Coast, District of Minahasa, North Sulawesi Province. This research was conducted in October 2019 until January 2020. Sampling used the roaming method in a predetermined location that is Atep Oki tidal/ intertidal coast. Sampling locations are spread over six coordinate points. Macroalgae found on the Atep Oki Coast assessed ten species belonging to three phyla, namely Chlorophyta (green algae), Ochrophyta (brown algae) and Rhodophyta (red algae). Chlorophyta members mean six species. Ochrophyta has members of three species and members of Rhodophyta only one species. The types of macroalgae found on the Atep Oki Coast provide bioactive compounds. Keywords : Macroalgae, Bioactive Compounds, Atep Oki Minahasa CoastAbstrak Penelitian ini bertujuan untuk menganalisis kekayaan jenis dan potensi senyawa bioaktif makroalga di Pesisir Atep Oki, Kabupaten Minahasa, Provinsi Sulawesi Utara. Penelitian ini telah dilaksanakan pada bulan Oktober 2019 sampai Januari 2020. Pengambilan sampel menggunakan metode jelajah di lokasi yang telah ditentukan yaitu pesisir pasang surut/intertidal Atep Oki. Lokasi pengambilan sampel tersebar di enam titik-titik koordinat. Makroalga yang ditemukan di Pesisir Atep Oki berjumlah sepuluh species yang tergolong ke dalam tiga filum yaitu Chlorophyta (alga hijau), Ochrophyta (alga cokelat) dan Rhodophyta (alga merah). Anggota Chlorophyta berjumlah enam species. Ochrophyta memiliki anggota tiga species dan anggota Rhodophyta hanya satu species. Jenis-jenis makroalga yang ditemukan di Pesisir Atep Oki berpotensi menghasilkan senyawa bioaktif. Kata kunci : Makroalga, Senyawa Bioaktif, Pesisir Atep Oki Minahasa

scholarly journals Phytochemical Screening and Antimicrobial Activity of Bundung Plants Extract by Dilution Method

2019 ◽  
Vol 5 (1) ◽  
pp. 143-154
Author(s):  
Noval Noval ◽  
Iwan Yuwindry ◽  
Dahlia Syahrina
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Inhibitory Activity ◽  
Screening Test ◽  
Ethanol Extract ◽  
Dilution Method ◽  
Phytochemical Screening ◽  
Extract Concentration ◽  
Convincing Data ◽  
Plants Extract

Bundung plants (Actinuscirpus Grossus) are widely spread in Borneo and used by society empirically as antimicrobials. Nevertheless, the use of Bundung plants as traditional medicine has not been equipped with convincing data, because there is no research that is related to the plants. In order to the use of the plants accountable, it is necessary to conduct research about phytochemical screening studies and tests the antimicrobial activity of ethanol extract of Bundung plants to staphylococcus aureusandEschericia Coli bacteria. Moreover, extraction does with maceration method. Secondary metabolite groups which are contained in the ethanol extract of Bundung plants were determined qualitatively using several of phytochemical reagents. The result of phytochemical screening test showed that ethanol extract of Bundung plants contains a group of secondary metabolites; namely flavonoid, tannin, saponin, phenolic, steroid and terpenoid. The method that was used to test antimicrobial activity to Staphylococcus aureusandEschericia Coli bacteria was a liquid dilution method with variations in extract concentration of 0,5%, 1%, 2%, 4% and 8% by considering at the clarity of each sample. The result of antimicrobial activity of liquid dilution to bacteria had inhibition at all concentrations and the biggest inhibitory activity was shown at concentration 8% with the clearest level and the MIC grade of the test is at a concentration of 1%. Based on the findings, it can be concluded that ethanol extract of Bundung plants has the potential of activities of an antimicrobial, especially from secondary flavonoid metabolites.

scholarly journals A Preliminary Screening of the Different of Secondary Metabolites Ruku-Ruku Leaves (Ocimum tenuiflorum Linnen) in West Sumatera

2019 ◽  
Vol 20 (2) ◽  
pp. 17-24
Author(s):  
Hesty Parbuntari ◽  
Sri Benti Etika ◽  
Melindra Mulia ◽  
Elfanny Delvia
Keyword(s):  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Light Exposure ◽  
Phytochemical Screening ◽  
Preliminary Screening ◽  
West Sumatra ◽  
Ocimum Tenuiflorum ◽  
Qualitative Test

Ruku-ruku leaves are used to remove distinctive odors from fish in some cuisine of West Sumatra. The characteristic odor comes from secondary metabolites especially essential oils. Therefore, secondary metabolites in ruku-ruku leaves have the potential to be further investigated as a source of bioactive compounds. The phytochemical screening of ruku-ruku leaves is a preminilary qualitative test that needs to be done to maximise its function as a medicinal plant. Based on the result data, there are some different result of flavonoid, steroids, and triterpenoids identification. Ruku-ruku leaves from Pesisir Selatan and Padang Panjang shows less flavonoid compounds tham Payakumbuh, Batusangkar, and Lubuk Alung. Both of this city and Payakumbuh also gives less steroids and triterpenoids. The factor influencing this result is caused by several factors, namely temperature, humidity, light exposure, and salinity.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAUN BENALU KOPI (Loranthus Ferrugineus Roxb.) DENGAN METODE DPPH (1,1 – Difenil -2- Pikrilhidrazil)

2019 ◽  
Vol 6 (2) ◽  
pp. 192
Author(s):  
Muammar Yulian ◽  
Safrijal Safrijal
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Secondary Metabolites ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Ic50 Values ◽  
X 24

The study about antioxidant activity test of coffee parasite leaves (Loranthus ferrugineus Roxb.) by 1,1-diphenyl-2-pikrilhidrazyl (DPPH) method has been done. The aim of this study was to determine the content and activity of secondary metabolites, flavonoids and antioxidant, which found in the coffee parasite leaves. Dry powder of parasite coffee leaves (Loranthus ferrugineus Roxb.) as much as 0.5 kg were macerated by 2 L of ethanol solvent at room temperature for 4 x 24 hours, then mixed and filtered. Ethanol filtrate was evaporated at 30-40°C by using a rotary evaporator to obtain the crude extract of coffee parasite leaves. The results of the phytochemical screening showed positively that the extract was containing alkaloid, flavonoids, saponins, tannins and steroid compounds.The results of the antioxidant activity test by using DPPH method was obtained that the amount of antioxidant activity of the samples of ethanol extract had a very strong antioxidant activity against radical DPPH 0.05 mM, with IC50 values was obtained 6.063 ppm. Whereas, for comparison of ascorbic acid was about 3.127 ppm.

scholarly journals Uji Daya Hambat Ekstrak Etanol, Fraksi n-heksana dan Etanol Daun Ceremai (Phyllanthus acidus L.) Skeels) Terhadap Salmonella thypi

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Sernita Sernita ◽  
Irnawati Irnawati ◽  
Syamsinar Syamsinar
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Test Method ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Herbal Plants ◽  
Phyllanthus Acidus ◽  
Ethanol Fraction

ABSTRAK Penyakit yang sering diobati dengan tanaman herbal adalah infeksi yang disebabkan oleh bakteri. Salah satu tanaman yang dapat dimanfaatkan sebagai obat herbal adalah daun ceremai. Penelitian ini bertujuan untuk mengetahui metabolit sekunder dan zona hambat ekstrak etanol, fraksi n-heksana dan etanol daun ceremai (Phyllanthus acidus L.) skeels) terhadap Salmonella thypi. Penyarian daun ceremai dengan metode maserasi menggunakan pelarut etanol 96% dilanjutkan fraksinasi dengan pelarut n-heksana dan etanol. Hasil ekstrak dan fraksi daun ceremai kemudian dilakukan uji skrining fitokimia untuk mengetahui senyawa metabolit sekunder yang terdapat dalam sampel. Metode pengujian aktivitas antibakteri yang digunakan dalam penelitian ini adalah metode sumuran. Berdasarkan hasil penelitian, skrining fitokimia ekstrak etanol dan fraksi etanol mengandung senyawa saponin, tanin, flavonoid dan alkaloid sedangkan fraksi n-heksana mengandung senyawa tanin, flavonoid dan alkaloid. Aktivitas antibakteri metode sumuran ekstrak dan fraksi daun ceremai dengan konsentrasi 15%, 20%, dan 25% memiliki diameter rata-rata zona hambat secara berturut-turut adalah ekstrak etanol yaitu 13,24 mm, 13,74 mm, dan 14,13 mm, Fraksi n-heksan 13,806 mm, 14,32 dan 14,763 mm dan Fraksi etanol 13,096 mm, 13,416 mm, dan 13,486 mm. Hasil identifikasi aktivitas antibakteri ekstrak etanol, fraksi n-heksana dan fraksi etanol menunjukkan fraksi n-heksana memiliki zona hambat yang paling tinggi walaupun masih tergolong dalam zona hambat kategori lemah. Kata Kunci: Daun Ceremai, Ekstrak Etanol, Fraksi n-Heksana, Fraksi Etanol Salmonella thypi. ABSTRACT Diseases that are often treated with herbal plants are infections caused by bacteria. One plant that can be used as an herbal medicine is ceremai leaves. This study aims to determine secondary metabolites and inhibitory zones of ethanol extract, n-hexane fraction and etanol fraction of ceremai leaves (Phyllanthus acidus L.) Skeels) on Salmonella thypi. The extraction of ceremai leaves by maceration method used 96% ethanol was continued by fractionation with n-hexane and ethanol solvents. The results of extracts and fraction of ceremai leaves were then carried out by phytochemical screening test to determine the secondary metabolites found in the sample. Antibacterial activity test method used in this study is the method of wells. Based on the results of the study, phytochemical screening of ethanol extract and ethanol fraction contained saponin, tannin, flavonoid and alkaloid compounds while the n-hexane fraction contained tannin, flavonoid and alkaloid compounds. Antibacterial activity of wells method of ceremai leaf extract and fraction with a concentration of 15%, 20%, and 25% had an average inhibitory zone diameter, respectively, of ethanol extract 13,24 mm, 13,74 mm, and 14,13 mm, n-hexane fraction 13,806 mm, 14,32 mm and 14,763 mm and ethanol fraction 13,096 mm, 13,416 mm, and 13,486 mm. The results of the identification of antibacterial activity of ethanol extract, n-hexane fraction and ethanol fraction showed that the n-hexane fraction had the highest inhibition zone although it was still classified as a weak inhibition zone. Keywords : Ceremai Leaf, Ethanol Extract, n-Hexane Fraction, Salmonella thypi.

scholarly journals PROFIL FITOKIMIA EKSTRAK ETANOL DAUN ANTING – ANTING (ACALYPHA INDICA LINN) DI KOTA KUPANG, NTT

2020 ◽  
Vol 8 (2) ◽  
pp. 153-163
Author(s):  
Meity Marviana Laut ◽  
Nemay Ndaong ◽  
Filphin Amalo ◽  
Larry Toha ◽  
Herlina Umbu Deta
Keyword(s):  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Extraction Method ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Local People ◽  
Phytochemical Screening ◽  
Plant Family ◽  
Local Use ◽  
Acalypha Indica

Acalypha indica Linn is a tropical weed, grows annually in East Nusa Tenggara. The weed is member of Euphorbiaceae family, a largest plant family known as medicinal plant. The weed leaves were used by local people in NTT to treat wounds, diseases or myasis on their livestock. This study aim to investigate the secondary metabolites in A. indica L leaves as a scientific proven for its local use.  The extract preparation comprises of several steps, i.e collection of fresh leaves, dry and wet sortation. The clean leaves were air dried in a room temperature for about 2 weeks before grounded into powder and subjected to extraction. The extraction method was maceration with ethanol 96% as solvent. The dense extract was evaporated using rotary evaporator and subjected to phytochemical screening. The result shows that ethanol extract of A.indica leaves were tested positive for flavonoid and tannin. Alkaloid, saponins, triterpenes and steroid were tested negative on the extract.

scholarly journals Pengaruh Suhu dan Lama Ekstraksi dengan cara Maserasi terhadap Karakteristik Pewarna dari Ekstrak Alga Merah (Gracilaria sp.)

2019 ◽  
Vol 7 (4) ◽  
pp. 488
Author(s):  
Novia Esterulina Purba ◽  
Lutfi Suhendra ◽  
Ni Made Wartini
Keyword(s):  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Red Algae ◽  
Block Design ◽  
Effect Of Temperature ◽  
Color Intensity ◽  
Sodium Potassium ◽  
Randomized Block Design ◽  
Effect On Yield ◽  
C 50

Red algae (Rhodophyta) contains vitamins, minerals, fiber, sodium, potassium and bioactive compounds in the form of secondary metabolites and the most important nutrients are pigments.This study aims to determine the effect of temperature and length of extraction by maceration on the characteristics of dyes from red algae extract (Gracilaria sp.) spand determine the best temperature and maceration time to produce the color extract of Gracilaria sp. This study used Factorial Randomized Block Design. The first factor is temperature which consists of 3 levels namely 30±1°C, 40±1°C, 50±1°C. The second factor is maceration length which consists of 3 levels, namely 24 hours, 30 hours and 36 hours. Each treatment was grouped into 2 based on the time of implementation so that 18 units of experiment were obtained. The variables observed were yield, phycoeritrin, color intensity and effectiveness test. The data obtained were then analyzed by variance analysis and test Tukey’s. The results showed that the treatment of temperature, maceration time and interactions between treatments had a very significant effect on yield, phycoerythrin, brightness (L*), redness level (a*) and yellowness level (b*). The temperature of 30±1°C and the maceration time of 30 hours is the best treatment to produce dyes from red algae extract (Gracilaria sp.) with the characteristics of yield 0.496 ± 0.001%, phycoerythrin 0.430 ± 0.006 mg/g, lightness (L*) 20.470 ± 0.141, redness level (a*) 21.790 ± 0.198 and yellowish level (b*) 12.205 ± 0.035. Keywords: temperature, length of maceration, color, phycoeritrin, Gracilaria sp.

scholarly journals UJI TOKSISITAS DAN SKRINING FITOKIMIA TEPUNG GABAH PELEPAH AREN (Arenga pinnata)

2012 ◽  
Vol 12 (2) ◽  
pp. 127
Author(s):  
Meiske S Sangi ◽  
Lidya I Momuat ◽  
Maureen Kumaunang
Keyword(s):  
Secondary Metabolites ◽  
Traditional Medicine ◽  
Toxicity Test ◽  
Research Method ◽  
Artemia Salina ◽  
Detection Time ◽  
Phytochemical Screening ◽  
Skin Burn ◽  
Lc50 Value ◽  
North Sulawesi

UJI TOKSISITAS DAN SKRINING FITOKIMIA TEPUNG GABAHPELEPAH AREN (Arenga pinnata)ABSTRAKTelah dilakukan penelitian uji toksisitas dan skrining fitokimia tepung pelepah aren. Tepung pelepah aren secara tradisional digunakan oleh masyarakat Tomohon sebagai obat untuk menghilangkan gatal-gatal dan luka bakar pada kulit, namun pemanfaatan ini belum dikenal secara luas di Sulawesi Utara. Pemanfaatan tepung pelepah aren sebagai obat tradisional ini belum banyak dilaporkan dalam literatur bahkan belum ada yang meneliti tentang sifat toksik dan kandungan senyawa metabolit sekunder yang terdapat pada tepung pelepah aren. Tujuan penelitian dalam pengujian toksisitas adalah untuk mengetahui apakah tepung pelepah aren bersifat toksik atau tidak selanjutnya menentukan nilai LC50-nya untuk mengetahui jumlah konsentrasi penyebab ketoksikan tepung pelepah aren. Metode yang digunakan untuk pengujian toksisitas adalah dengan menggunakan larva udang jenis Artemia salina Leach. Dalam metode ini A. salina Leachdipakai sebagai bioindikator. Metode ini mudah dikerjakan, murah, waktu deteksi singkat dan dapat dipertanggungjawabkan. Hasil yang diperoleh dari ekstrak etanol tepung pelepah aren bersifat toksik dengan nilai LC50-nya adalah 6,295 ppm dan hasil pengujian fitokimia menunjukkan bahwa tepung pelepah aren mengandungsenyawa metabolit sekunder yaitu alkaloid yang ditunjukkan dengan pembentukan endapan jingga ketika direaksikan dengan pereaksi dragendorf, triterpenoid yang ditunjukkan dengan perubahan warna jingga pada sampel dan tanin yang ditunjukkan dengan pembentukan endapan putih ketika direaksikan Gelatin.Kata kunci: pelepah aren, skrining fitokimia dan uji toksisitasTOXICITY TEST AND PHYTOCHEMICAL SCREENING ONPALM SUGAR LEAF MIDRIB FLOUR (Arenga pinnata)ABSTRACTA study of toxicity test and phytochemical screening on sugar palm midrib flour had been conducted. The flour is traditionally used as medicine to relieve itching and skin burn, although the use is not widely known in North Sulawesi. Utilization of this flour as a traditional medicine had not been widely reported in the literatures and the toxicity and content of secondary metabolites contained in the sugar palm midrib had not been studied. The purpose of this research was to determine the toxicity of the flour and the value of LC50 in order to find the concentration that causes its toxicity. The research method involved the use of Artemia salina Leach shrimp larvae as a bioindicator. This method was easy to perform, inexpensive, had short detection time, and reliable. The results showed that the ethanol extracted flour was toxic and had LC50 value of 6,295 ppm. The phytochemical screening showed that the flour contained secondary metabolite of alkaloid which was indicated by orange precipitation by using reagent Dragendorf, of triterpenoid which was indicated by orange discoloration, and of tannin which was indicated by white precipitate by using gelatin.Keywords: sugar palm midrib, phytochemical screening and toxicity test

scholarly journals Phytochemical Screening of Sunflower Leaf (Helianthus annuus) and Anting-Anting (Acalypha indica Linn) Plant Ethanol Extract

2019 ◽  
Vol 2 (1) ◽  
pp. 24-30 ◽  
Author(s):  
Khoirul Ngibad
Keyword(s):  
Secondary Metabolites ◽  
Helianthus Annuus ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Traditional Medicines ◽  
Local Plants ◽  
Acalypha Indica

Many local plants in Indonesia were used as traditional medicines, such as sunflower (Helianthus annuus) and anting-anting (Acalypha indica Linn) plants. Both of them can be used as an antimalarial, antibacterial, antifungal, analgesic, and antihyperlipidemic. This study aims to provide an overview of the secondary metabolites groups contained in sunflower leaf and anting-anting plants for the testing of alkaloids, flavonoids, tannins, saponins, steroids/triterpenoids, and sesquiterpenoids. This study included the extraction of sunflower leaves and anting-anting plants separately using the maceration method for 24 hours with 80% ethanol solvent. The stirring was aided by a shaker for three hours. Each extract was tested by phytochemicals with reagents. The results of phytochemical tests with reagents showed that 80% ethanol extract of sunflower leaves contained an alkaloid, tannin, steroid, and sesquiterpenoid compounds whereas anting-anting plants contained an alkaloid, flavonoid, and triterpenoid compounds.

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