scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL AKAR SAMBILOTO (Andrographis paniculata (Burm.F.) Ness.) DENGAN METODE DPPH

2021 ◽  
Vol 4 (1) ◽  
pp. 20
Author(s):  
Yuri Pratiwi Utami
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanol Extract ◽  
The Body ◽  
Andrographis Paniculata ◽  
Radical Reactions ◽  
Root Extract ◽  
Free Radical Reactions ◽  
Traditional Medicinal Plants ◽  
Percentage Yield

AbstractAntioxidants are compounds that can inhibit free radical reactions in the body. One of Indonesia's traditional medicinal plants that have antioxidant activity is sambiloto (Andrographis paniculata (Brum) Ness.). This study aimed to examine the antioxidant activity of the extract of bitter root (A. paniculata (Brum) Ness.). The ethanol extract was obtained by extracting the bitter root powder by maceration for 3x24 hours and remaceration for 2x24 hours. The resulting extract is a thick blackish brown extract and has a distinctive odor with a percentage yield of 6.67%. Antioxidant activity was measured by reducing DPPH free radicals with UV-Vis spectrophotometry at a wavelength of 517 nm. The results showed that sambiloto root extract had very strong antioxidant activity with an IC5016,634 g/mL value against DPPH free radicals.Keywords : Bitter root, antioxidant, DPPH   AbstrakAntioksidan merupakan senyawa yang dapat menghambat reaksi radikal bebas dalam tubuh. Salah satu tumbuhan obat tradisional Indonesia yang memiliki aktivitas sebagai antioksidan adalah sambiloto (Andrographis paniculata (Brum) Ness.). Penelitian ini bertujuan untuk menguji aktivitas antioksidan ekstrak akar sambiloto (A. paniculata  (Brum) Ness.).  Ekstrak etanol diperoleh dengan mengektraksi serbuk akar sambiloto dengan cara maserasi selama 3x24 jam dan dilakukan remaserasi kembali selama 2x24 jam. Ekstrak yang dihasilkan merupakan ekstrak kental berwarna coklat kehitaman dan bau khas dengan persen rendamen sebesar 6.67 %. Aktivitas antioksidan diukur melalui peredaman radikal bebas DPPH dengan spektrofotometri UV-Vis pada panjang gelombang 517 nm. Hasil Penelitian menunjukkan ekstrak akar sambiloto yang  memiliki aktivitas antioksidan sangat kuat dengan nilai IC5016,634 µg/mL terhadap radikal bebas  DPPH.Kata Kunci : Akar sambiloto, antioksidan, DPPH

scholarly journals Analysis of antioxidant activity, total phenolic and flavonoid contents of ethanol extract of Litsea cubeba Lour. Bark

2021 ◽  
Vol 332 ◽  
pp. 08005
Author(s):  
Aminah Dalimunthe ◽  
Dewi Pertiwi ◽  
Mahatir Muhammad ◽  
Denny Satria
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanol Extract ◽  
The Body ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Litsea Cubeba ◽  
Ferric Reducing Antioxidant Power ◽  
Phenolic And Flavonoid

Free radicals also play a role in the pathology of various degenerative diseases such as cancer, rheumatism, coronary heart disease, cataracts, and others. Free radicals can come from within the body (endogenous) and outside the body (exogenous). Litsea cubeba (Lour,) is a Lauraceae family plant which have contents volatile oils which used as antimicrobial, anticancer on breast cancer, pesticide, antideppressants, antiinflammation, antioxidant, and neuro pharmacology. The extract was prepared using water with the soxhletation method. The antioxidant activity was determined with the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-Azinobis [3-ethylbenzothiazoline-6)-sulfonic acid] -diammonium salt (ABTS) and Ferric Reducing Antioxidant Power (FRAP) methods. Total flavonoid and total phenolic content were determined with colorimetric methods. Antioxidant activity measured as IC50 was 23.37 ± 0.42 µg/mL; 111.21 ± 0.42 and 109.01 ± 0.28 respectively. The extract was found to contain high levels of total phenolic (282.93 ± 0.33 mg GAE/g) and total flavonoid 7.49 ± 0.51 mg QE/g). The results reveal that ethanol extract of Litsea cubeba Lour. Bark has antioxidant potential. The further analysis is to isolation antioxidant compound.

scholarly journals INVESTIGATION OF NEUROPROTECTIVE ACTIVITY THIOPHANE INVOLUTIONAL CHORIORETINAL DEGENERATION IN RATS OXYS

2013 ◽  
Vol 12 (3) ◽  
pp. 24-32
Author(s):  
A. A. Zhdankina ◽  
G. A. Kon ◽  
M. B. Plotnikov ◽  
Ye. Yu. Varakuta ◽  
S. V. Logvinov ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Neuroprotective Activity ◽  
Radical Reactions ◽  
Percentage Increase ◽  
Total Antioxidant Activity ◽  
Retinal Neurons ◽  
Free Radical Reactions ◽  
Total Antioxidant ◽  
Chorioretinal Degeneration

The method of spontaneous and induced luminol -dependent chemiluminescence in homogenates of rat retinas OXYS registered increase in the generation of free radicals against decrease in total antioxidant  activity.  With  the  ultra-microscopic  and  quantitative  analysis  revealed  degenerative retinal neurons rats OXYS: the percentage increase in neurosensory cells with pyknosis of nuclei, hyperchromatic piknomorfnyh associative neurons and ganglion n eurons that have been modified on a light and dark type. Thiophane, limiting free radical reactions in the retina and protects retinal neurons from damage.

Invivo Protective Effect of Indigofera on Hepatic Tissue

2020 ◽  
Vol 8 (1) ◽  
pp. 7-10
Author(s):  
Vishal N Kushare ◽  
Suvarna A Wagh
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Human Body ◽  
Ethanol Extract ◽  
The Body ◽  
Hepatic Tissue ◽  
Cell Organelle ◽  
The World ◽  
Ethanol Extracts

Oxidative Stress is the primary cause of most of the diseases currently in the world. The human body is prone to many illnesses out of which oxidative stress plays a significant role in the causation of those. The Stress in the body releases free radicals and these free radicals react with the cell organelle and cause denaturation and break down. The generated free radicals should be fought to eliminate and prevent toxic effects. Many drugs are known to fight free radicals. Most of them had been scientifically evaluated and published for their activity too. The investigations say that antioxidant activity of the herbs does not similarly reflect . There were differences in activity and significant deviations in the results that are shown in comparison to and . Thus, there is an urgent necessity to investigate the antioxidant activity of herbs to make sure that the activity resembles and to find any variations when compared to activity. This research protocol was framed out to screen for the antioxidant activity of the ethanol extracts of the Indigofera leaves by estimating the serum parameters and correlating the results with the human body. Out of the values overall, the were the ones that are significant participants in fighting the free radicals and in boosting the immune system. The ethanol extract successfully prevented the breakdown of the cellular structure of the liver cells, thereby protecting it and replenishing the enzyme levels by destroying the free radicals that are generated due to DMH.

AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI AKAR TABAR KEDAYAN (Aristolochia foveolata Merr.) DENGAN METODE DPPH (2,2 DIPHENYL-1-PICRILHYDRAZIL)

2019 ◽  
pp. 8-14
Author(s):  
Risa Supringrum ◽  
Siti Jubaidah
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Hexane Fraction ◽  
High Dose ◽  
Water Fraction ◽  
Dpph Method

Antioxidants are substances that can neutralize free radicals, thus protecting the body from various diseases by binding to free radicals and highly reactive molecules that can damage cells. The use of high-dose synthetic antioxidants is reported to be toxic and carcinogenic. Tabar Kedayan root (Aristolochia foveolata Merr) is one of the native plants from North Kalimantan, which is empirically used as an anti-poison, containing secondary metabolites including alkaloids, tannins, flavonoids. Flavonoids are powerful antioxidants. Some studies report that the function of flavonoids can be to prevent and treat cancer. This study aims to determine the antioxidant activity of ethanol extract and n-hexane fraction, the ethyl acetate fraction of Tabar Kedayan root with 2.2 Diphenyl- 1-Picrilhydrazil (DPPH) method as a free radical compound. The results of the study obtained IC50 values for each sample, at ethyl acetate fraction 267.48 ppm, ethanol extract at 603.80 ppm, ethanol-water fraction 705.43 ppm, n- hexane fraction 1500 ppm. The antioxidant activity tests indicate that ethyl acetate fraction is categorized as weak antioxidant, while the ethanol-water fraction and n-hexane fraction exhibit no antioxidant activity. Keywords : Tabar Kedayan, Antioxidants, 2.2 Diphenyl-1-Picrilhydrazil

scholarly journals Antioxidant activity ethanol extract of gotu kola (Centella asiatica (L.) Urban) with DPPH method (2,2-Diphenyl-1-Pikrilhidrazil)

2020 ◽  
Vol 3 (2) ◽  
pp. 106
Author(s):  
Muhammad Ainul Yahya ◽  
Iif Hanifa Nurrosyidah
Keyword(s):  
Air Pollution ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
The Body ◽  
Antioxidant Compounds ◽  
Test Results ◽  
Ic50 Value ◽  
Dpph Method

Unhealthy lifestyles and air pollution cause the number of free radicals in the body to increase. To protect the body from free radicals, there are antioxidant compounds as an antidote and stabilize free radicals. One of the Indonesian plants that can be used as antioxidants is gotu kola (Centella asiatica (L.) Urban. Objective: This study aims to determine the antioxidant activity of the ethanol extract of gotu kola herb using the DPPH (2,2-Diphenyl-1-pikrilhidrazil) method. with IC50 value. Method: Gotu kola (Centella asiatica (L.) Urban) was extracted by the soxhletation method using 96% ethanol solvent. The testing of antioxidant activity was carried out using the DPPH (2.2 Diphenyl-1-Pikrihydrazil) method. Result: Test results of antioxidant activity The ethanol extract of gotu kola herb showed an IC50 value of 78.20 ppm. Conclusion: This indicated that the ethanol extract of gotu kola herb was included in the criteria for strong antioxidants. 

Antioxidant Activity and Total Phenolic Contents in Methanol Extracts from Swietenia Mahagoni and Andrographis Paniculata

2014 ◽  
Vol 69 (4) ◽  
Author(s):  
Liza Md Salleh ◽  
Hartati Hartati ◽  
Roslina Jamaludin ◽  
Mohd. Azizi Che Yunus ◽  
Harisun Yakub ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Soxhlet Extraction ◽  
Andrographis Paniculata ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Traditional Medicinal Plants ◽  
Percentage Yield ◽  
Swietenia Mahagoni

Traditional medicinal plants such as Swietenia mahagoni and Andrographis paniculata are rich with bioactive compounds. The phenolic composition and antioxidant activity of methanol extract from Swietenia mahagoni and Andrographis paniculata were studied. They were extracted by Soxhlet extraction of 70% methanol solvent. The results obtained from this study showed Andrographis paniculata gave highest percentage yield by 19.94% compared to Swietenia mahagoni at 11.86%. In antioxidant activity, Andrographis paniculata and Swietenia mahagoni showed 89.93% and 60.77% respectively. While for total phenolic content highest in extract of Swietenia mahagoni by 55.0 mg/g and Andrographis paniculata only 7.7 mg/g.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

PROSPECTS FOR USING ASTRAGALUS ONOBRYCHIS AS A SOURCE OF NATURAL ANTIOXIDANTS

2021 ◽  
pp. 47-53
Author(s):  
Pozdnyakovа Т.А. ◽  
Kuleshovа Е.S. ◽  
Bubenchikov R.A.
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Chemical Interaction ◽  
Raw Materials ◽  
Natural Antioxidants ◽  
The Body ◽  
Plant Raw Materials ◽  
Total Antioxidant ◽  
Promising Source ◽  
The Relationship

The predominance of synthetic and genetically modified foods in the diet of a modern person often leads to disruption of natural metabolic processes and the accumulation of toxic free radicals in the body, which provokes the development of various diseases. Natural antioxidants from medicinal plants can help maintain health and stop the processes of peroxidation. In our opinion, Аstragalus onobrychis can become a promising source of natural antioxidants. The aim of this study was to establish the antioxidant activity in extracts from the herb Astragalus onobrychis. In order to obtain objective and reliable results during our research, we used two methods: the method of titrimetry and spectrophotometry of free radicals. The titrimetric method is based on the chemical interaction between an oxidizing agent (potassium permanganate) and natural antioxidants present in extracts from plant raw materials, since they exhibit the properties of reducing agents. The spectrophotometric method makes it possible to assess the value of antioxidant activity and the duration of the reaction by assessing the interaction of 2,2-diphenyl-1-picrylhydrazil with plant antioxidants. To establish the relationship between the antioxidant activity and the presence of phenolic compounds in the plant, the content of flavonoids and hydroxycinnamic acids in the herb Astragalus onobrychis was determined. As a result of the studies, the total antioxidant activity of extracts from the herb Astragalus onobrychis was established and it was revealed that its value depends on the type of extractant used, since this factor is associated with the content of various groups of natural antioxidants in the extraction. The data obtained indicate the prospects of using the herb Astragalus onobrychis as a source of natural antioxidants.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SPONS Stylissa sp. DENGAN MENGGUNAKAN METODE DPPH (1,1-difenil-2-pikrilhidrazil)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 419
Author(s):  
Samuel I.M. Sibarani ◽  
Adithya Yudistira ◽  
Deby A. Mpila
Keyword(s):  
Antioxidant Activity ◽  
Cell Wall ◽  
Free Radicals ◽  
Ethanol Extract ◽  
Dna Bases ◽  
Oxidation Reactions ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
High Antioxidant Activity ◽  
Ethanol Extracts

ABSTRACTHabitat of sponge Stylissa sp., were under the sea and these sponges contain active compounds, which are more active than the compounds produced by terrestrial plants. Antioxidants are inhibitors of oxidation reactions due to the free radicals, which can cause weak damage to unsaturated cells, cell wall membranes, blood vessels, DNA bases, and lipid tissue, that causing disease. The study was conducted to determine the antioxidant activity of ethanol extracts  of sponge Stylissa sp., which is located on the Bangka Island, Likupang District, North Minahasa Regency. Sponge Stylissa sp., was extracted by maceration with using ethanol. Testing of antioxidant activity was carried out by the DPPH method measured by a UV-Vis spectrophotometer. The results of the study showed that ethanol extracts of sponge Stylissa sp., has antioxidant activity in each concentration and the highest at a concentration of 100 mg / L. The conclusion is a ethanol extract of sponge Stylissa sp. have high antioxidant activity with a concentration of 25 mg/L (77,40%), concentration of 50 mg/L (85,63%), concentration of 75 mg/L (88,66%), and concentration 100 mg/L (88,96%). Key words: Stylissa sp. Sponge, Antioxidant, DPPH (1,1-diphenyl-2-picrylhydrazyl) ABSTRAKHabitat dari spons Stylissa sp. terdapat di bawah laut dan spons ini mengandung senyawa aktif yang persentase keaktifannya lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Antioksidan adalah zat penghambat reaksi oksidasi akibat radikal bebas yang dapat menyebabkan kerusakan lemah tak jenuh, membran dinding sel, pembuluh darah, basa DNA, dan jaringan lipid sehingga menimbulkan penyakit. Penelitian dilakukan untuk mengetahui aktivitas antioksidan ekstrak etanol biota laut spons Stylissa sp. yang terdapat di pulau Bangka, Kecamatan Likupang, Kabupaten Minahasa Utara. Spons Stylissa sp. ini diekstrak dengan metode maserasi menggunakan etanol. Pengujian aktivitas antioksidan ini dilakukan dengan metode DPPH yang diukur dengan alat spektrofotometer UV-Vis. Hasil dari penelitian menunjukan bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan disetiap konsentrasi dan yang tertinggi pada konsentrasi 100 mg/L. Kesimpulan yang didapat bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan yang tinggi dengan konsentrasi 25 mg/L (77,40%), konsentrasi 50 mg/L (85,63%), konsentrasi 75 mg/L (88,66%), dan konsentrasi 100 mg/L (88,96%). Kata Kunci : Spons Stylissa sp., Antioksidan, DPPH (1,1-difenil-2-pikrilhidrazil)

scholarly journals A Preliminary Up-To-Date Review on Pakistani Medicinal Plants with Potential Antioxidant Activity

2020 ◽  
Vol 11 (1) ◽  
pp. 1-27
Author(s):  
Adil Hussain
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Medicinal Plants ◽  
Natural Antioxidants ◽  
The Body ◽  
New Drugs ◽  
Lung Damage ◽  
Present Review ◽  
Experimental Investigations ◽  
Health Related

Background: There exist natural antioxidants in plants that scavenge harmful free radicals from the body. Free radicals are species of chemical origin with an unpaired electron and play a pivotal role in combating against health-related problems like lung damage, inflammation, and cardiovascular ailments etc. Antioxidants halt the development of these free radicals called the reactive oxygen species either by chelating the trace elements or by enzymes inhibition. Objectives: The aim of present review was to collect information about Pakistani medicinally important plants with the exploration of their antioxidant potential. Methodology: Total 206 papers were looked over, which were obtained from numerous sources like; Google Scholar, Medline, PubMed, Research Gate, Science Direct, Scopus and Web of Science. Results: Overall, 93 plants representing 44 families with potential antioxidant activity reported from Pakistan have been presented in this review. Maximum number of species from Asteracea, Poaceae and Rutaceae familes were scrutinized for their potential antioxidant activity from Pakistan. Conclusion: The present review clearly designates that the presence of phytochemicals and antioxidant activity of medicinal plants from Pakistan vary with the species of the plants and material/extracts used. From this review, it is recommended to perform comprehensive experimental investigations based on toxicology and ethnopharmacology on these precious plants from Pakistan. It will be advantageous in the provision of trustworthy information to patients and determine further innovative compounds for safer and new drugs development with fewer side effects.

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