scholarly journals EFFECT OF BIOSTIMULANTS ON SOME BIOACTIVE COMPOUNDS AND NITRATE LEVEL IN LETTUCE (Lactuca sativa L.) GROWN UNDER UNHEATED PLASTIC TUNNEL

2021 ◽  
Vol 52 (6) ◽  
pp. 1318-1325
Author(s):  
A. A. Yaseen ◽  
M. T. Hajos
Keyword(s):  
Vitamin C ◽  
Bioactive Compounds ◽  
Plant Protection ◽  
Nutrient Content ◽  
Bark Extract ◽  
Nitrate Content ◽  
Nitrate Level ◽  
Salix Alba ◽  
Total Polyphenols ◽  
Indole Butyric Acid

Based on the regulation (EU) No 2015/1107 in 8 July 2015, Willow (Salix alba) bark extract is a recent approved basic substance in Europe as a safe and effective environmentally friendly fungicide to be used in plant protection. Because of having indole butyric acid content, it is also used as a growth hormone to promote rooting in cutting propagation of wooden part in horticultural plants. This research aimed to evaluate the effect of willow extract and Ferbanat L (Bistep) on lettuce leaves. Bistep is an organic solution of nano fertilizer which is produced in Hungary and allowed as „Bistep plant conditioner”. Furthermore, the experiment evaluated their interaction on some bioactive compounds in different lettuce varieties, too. Three different lettuce varieties (King of May, Kobak and Great Lakes) were evaluated for total polyphenols, vitamin C and nitrate content in fresh leaves. The results indicate that the contents were significantly affected by the used biostimulants rather than varieties. Significantly difference of the total polyphenols was recorded in the plants treated with Willow + Bistep (80.64 mg GAE / 100g) in comparison to the control (55.85 mg GAE / 100g) in the mean of varieties. Similar result was recorded for the vitamin C. However, plants treated with Willow extract resulted in the highest level of nitrate content (692.00 mg/100g), while the lowest amount was measured in the control (488.00 mg/100g). According to our results, Willow bark extract can be a beneficial source of natural growth regulator or fungicide; however, when it is used alone for vegetables, the nutrient content (mainly nitrate level) must be checked before consuming and delivering to the market.

scholarly journals Nutraceutical Analysis of Marticaria recutita (Chamomile) Dried Leaves and Flower Powder and Comparison between Them

2018 ◽  
Vol 10 (2) ◽  
pp. 111
Author(s):  
Ekta Singh Chauhan ◽  
Jaya Aishwarya
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Aqueous Extract ◽  
Nutrient Content ◽  
Different Types ◽  
Anti Oxidant ◽  
Chamaemelum Nobile ◽  
Anti Inflammatory

<p>Chamomile is known as German Chamomile (Marticaria recutita) and Roman Chamomile (Chamaemelum nobile) a very famous daisy plant. The work mainly focuses on the nutraceuticals potential of Chamomile leaf and flower of this plant. The nutrient contains of the leaf and flower power was determined by various methods. The phytochemicals screening of the leaf and flower aqueous extract was performed by the different procedure. Leaf of this plant is rich in carbohydrate, protein, fat and also rich in vitamin C, iron, zinc and calcium. Whereas flower is rich in moisture and fiber as compared to leaf. The aqueous extract of leaf of Chamomile showed the presence of steroids, terpenoids, flavonoids, tannins and saponins and flower were lacked in alkaloids, saponins, gelatin and phenolic compounds. The results record that leaf and flowers powder contains different types of nutrients and phytochmicals in it.<strong> </strong>Chamomile is rich in different bioactive compounds, antioxidant and phytochemicals; carries many pharmacological and traditional properties. Leaves, flowers and stems of Chamomile are used as anti-oxidant, analgesic, anti-viral, anti-inflammatory, anti-septic, anti-diabetic, anti-proliferative, anti-bacterial activities and many more diseases.<strong> </strong>This paper put a light on nutrient content and phytochemical properties of Chamomile leaf and flower.</p>

scholarly journals Potential of Pre-Harvest Wastes of Tobacco (Nicotiana tabacum L.) Crops, Grown for Smoke Products, as Source of Bioactive Compounds (Phenols and Flavonoids)

2021 ◽  
Vol 13 (4) ◽  
pp. 2087
Author(s):  
Maria Isabella Sifola ◽  
Linda Carrino ◽  
Eugenio Cozzolino ◽  
Luisa del Piano ◽  
Giulia Graziani ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Dry Weight ◽  
Waste Biomass ◽  
Total Polyphenols ◽  
Nicotiana Tabacum L ◽  
Diammonium Salt ◽  
Q Exactive ◽  
Tobacco Cultivation ◽  
Folin Assay ◽  
Final Harvest

Tobacco cultivation is characterized by high amounts of waste biomasses whose disposal frequently represents a complex and expensive problem. A study was conducted to evaluate thepotential of pre-harvest light air-cured (Burley) and dark fire-cured (Kentucky) tobacco waste biomasses as a source of bioactive compounds (nutraceutical ingredients) such as polyphenols. Pre-harvest waste materials (topping fresh materials and residual stalks at final harvest) were collected to determine dry matter, total polyphenols content (TPC; Folin assay), and DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,20-azino-bis(3-ethylbenzothiazoline-60-sulfonic acid) diammonium salt) antioxidant capacity. Polyphenols quali-quantitative profiles obtained by Orbitrap Q Exactive of both tobacco types were also determined. Total pre-harvest waste biomass amounted to 3956.9 and 1304.4 kg d.w. ha−1 in light air-cured (Burley) and dark fire-cured (Kentucky) tobacco types, respectively. Polyphenols content, expressed as g kg−1 dry weight (d.w.), ranged between 4.6 and 15.7 g kg−1 d.w. and was generally greater in leaves than in stalks. Considering both leaves and stalks, the light air-cured (Burley) tobacco crop yielded 22.1 kg ha−1 of polyphenols, while the dark fire-cured (Kentucky) tobacco yielded 12.0 kg ha−1. DPPH and ABTS were significantly greater in leaves than in stalks waste biomass in both types of tobacco. The most abundant components were quinic and chlorogenic acids, rutin, and luteolin rutinoside.

scholarly journals Comparative Assessment of the Basic Chemical Composition and Antioxidant Activity of Stevia rebaudiana Bertoni Dried Leaves, Grown in Poland, Paraguay and Brazil—Preliminary Results

2021 ◽  
Vol 11 (8) ◽  
pp. 3634
Author(s):  
Teresa Leszczyńska ◽  
Barbara Piekło ◽  
Aneta Kopeć ◽  
Benno F. Zimmermann
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Vitamin C ◽  
Dietary Fiber ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Total Polyphenols ◽  
Stevia Rebaudiana Bertoni

This study compares the content of basic nutrients (proteins, fats, digestible carbohydrates, dietary fiber and ash), steviol glycosides, selected antioxidants (vitamin C, total polyphenols) and antioxidant activity in dried leaves of Stevia rebaudiana Bertoni cultivated in Poland, Paraguay and Brazil and available in the direct sale. The basic chemical composition was determined by standard AOAC (Association of Official Analytical Chemists) methods. Content of steviol glycosides was determined by the UHPLC-UV chromatographic method. Total polyphenols content was expressed as gallic acid equivalent (GAE) and catechins equivalent (CE). Antioxidant activity was measured as ABTS●+ free radical scavenging activity. Dried leaves of S. rebaudiana grown in Poland had significantly higher contents of dietary fiber, and lower protein and ash content, compared to those derived from Paraguay and Brazil. The former had, however, considerably higher contents of total steviol glycosides, stevioside and rebaudioside D, compared to the remaining two plants. In the Paraguay-derived dried leaves, the content of rebaudioside A, C, E and rubusoside was found to be significantly lower. Dried leaves of S. rebaudiana Bertoni, cultivated in Poland, contained substantially more vitamin C and a similar content of total polyphenols, compared to those from Brazil and Paraguay. The examined material from Brazil and Paraguay plantations showed similar antioxidant activity, while that obtained from Polish cultivation was characterized by a significantly lower value of this parameter.

scholarly journals EC Sensitivity of Hydroponically-Grown Lettuce (Lactuca sativa L.) Types in Terms of Nitrate Accumulation

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 315
Author(s):  
Noémi Kappel ◽  
Ildikó Fruzsina Boros ◽  
Francia Seconde Ravelombola ◽  
László Sipos
Keyword(s):  
Lactuca Sativa ◽  
Nitrate Content ◽  
Saline Condition ◽  
Fresh Weight ◽  
Nitrate Level ◽  
Nitrate Accumulation ◽  
Strong Negative Correlation ◽  
Lactuca Sativa L ◽  
Hydroponically Grown ◽  
European Markets

The goal of this research was to investigate the effect of electrical conductivity (EC) levels of the nutrient solution on the fresh weight, chlorophyll, and nitrate content of hydroponic-system-grown lettuce. The selected cultivars are the most representative commercial varieties grown for European markets. Seven cultivars (‘Sintia,’ ‘Limeira,’ ‘Corentine,’ ‘Cencibel,’ ‘Kiber,’ ‘Attiraï,’ and ‘Rouxaï’) of three Lactuca sativa L. types’ (butterhead, loose leaf, and oak leaf) were grown in a phytotron in rockwool, meanwhile the EC level of the nutrient solutions were different: normal (<1.3 dS/m) and high (10 dS/m). The plants in the saline condition had a lower yield but elevated chlorophyll content and nitrate level, although the ‘Limeira’ and ‘Cencibel’ cultivars had reduced nitrate levels. The results and the special characteristic of the lollo-type cultivars showed that the nitrate level could be very different due to salinity (‘Limeira’ had the lowest (684 µg/g fresh weight (FW)) and ‘Cencibel’ had the highest (4396 µg/g FW)). There was a moderately strong negative correlation (−0.542) in the reverse ratio among the chlorophyll and nitrate contents in plants treated with a normal EC value, while this relationship was not shown in the saline condition. Under the saline condition, cultivars acted differently, and all examined cultivars stayed under the permitted total nitrate level (5000 µg/g FW).

scholarly journals The Effect of Ripening Stages on the Accumulation of Carotenoids, Polyphenols and Vitamin C in Rosehip Species/Cultivars

2021 ◽  
Vol 11 (15) ◽  
pp. 6761
Author(s):  
Brigita Medveckienė ◽  
Jurgita Kulaitienė ◽  
Dovilė Levickienė ◽  
Ewelina Hallmann
Keyword(s):  
High Performance Liquid Chromatography ◽  
Vitamin C ◽  
High Performance ◽  
Hplc Method ◽  
Total Carotenoid ◽  
Ripening Stage ◽  
Rosa Rugosa ◽  
Total Polyphenols ◽  
Rosa Canina ◽  
Ripening Stages

Our research was aimed at assessing the effect of accumulation of carotenoids, polyphenols, vitamin C and ripening stage in the rosehip fruits of two species—Rosa canina, Rosa rugosa and two cultivar—Rosa rugosa ‘Rubra’ and Rosa rugosa ‘Alba’. The amounts of carotenoids, polyphenols and vitamin C were determined using the high-performance liquid chromatography (HPLC) method. The obtained results showed that the significantly highest amount (107.15 mg 100 g−1) of total carotenoid was determined in the fruits of Rosa canina at ripening Stage V. While results indicated that significant amount of total polyphenols were established at Stages I and II in the Rosa Rugosa ‘Alba’ and Rosa rugosa ‘Rubra’ cultivars (110.34 mg 100 g−1, 107.88 mg 100 g−1 and 103.20 mg 100 g−1 103.39 mg 100 g−1). At ripening Stage I, in the fruits of Rosa rugosa the greatest increases were established in the contents of vitamin C (3036.08 mg 100 g−1).

scholarly journals The Use of a Micro Near Infrared Portable Instrument to Predict Bioactive Compounds in a Wild Harvested Fruit—Kakadu Plum (Terminalia ferdinandiana)

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1413
Author(s):  
Eshetu Bobasa ◽  
Anh Dao T. Phan ◽  
Michael Netzel ◽  
Heather E. Smyth ◽  
Yasmina Sultanbawa ◽  
...  
Keyword(s):  
Vitamin C ◽  
Bioactive Compounds ◽  
Ellagic Acid ◽  
Near Infrared ◽  
Wide Range ◽  
Fruit Samples ◽  
Nutritional Characteristics ◽  
Analytical Quantification ◽  
Kakadu Plum ◽  
Terminalia Ferdinandiana

Kakadu plum (KP; Terminalia ferdinandiana Exell, Combretaceae) is an emergent indigenous fruit originating from Northern Australia, with valuable health and nutritional characteristics and properties (e.g., high levels of vitamin C and ellagic acid). In recent years, the utilization of handheld NIR instruments has allowed for the in situ quantification of a wide range of bioactive compounds in fruit and vegetables. The objective of this study was to evaluate the ability of a handheld NIR spectrophotometer to measure vitamin C and ellagic acid in wild harvested KP fruit samples. Whole and pureed fruit samples were collected from two locations in the Kimberley region (Western Australia, Australia) and were analysed using both reference and NIR methods. The standard error in cross validation (SECV) and the residual predictive deviation (RPD) values were 1.81% dry matter (DM) with an RPD of 2.1, and 3.8 mg g−1 DM with an RPD of 1.9 for the prediction of vitamin C and ellagic acid, respectively, in whole KP fruit. The SECV and RPD values were 1.73% DM with an RPD of 2.2, and 5.6 mg g−1 DM with an RPD of 1.3 for the prediction of vitamin C and ellagic acid, respectively, in powdered KP samples. The results of this study demonstrated the ability of a handheld NIR instrument to predict vitamin C and ellagic acid in whole and pureed KP fruit samples. Although the RPD values obtained were not considered adequate to quantify these bioactive compounds (e.g., analytical quantification), this technique can be used as a rapid tool to screen vitamin C in KP fruit samples for high and low quality vitamin C.

scholarly journals Bioactive Compounds, Antioxidant, and Antibacterial Properties of Lonicera caerulea Berries: Evaluation of 11 Cultivars

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 624
Author(s):  
Laima Česonienė ◽  
Juozas Labokas ◽  
Ina Jasutienė ◽  
Antanas Šarkinas ◽  
Vilma Kaškonienė ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Vitamin C ◽  
Organic Acids ◽  
Bioactive Compounds ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Lonicera Caerulea ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Ethanolic Extracts

The aim of the study was to evaluate 11 cultivars of blue honeysuckle (Lonicera caerulea L.) for bioactive compounds, antioxidant capacity, and the antibacterial activity of berries. Total phenolic contents (TPCs) and total anthocyanin contents (TACs) were established by using ethanolic extracts. For contents of organic acids and saccharides, aqueous extracts were used, and vitamin C was determined by using oxalic acid solution. DPPH• radical scavenging capacity was evaluated by using ethanolic extracts; antibacterial activity was assessed by using both ethanolic and aqueous extracts. The TPC varied from 364.02 ± 0.41 mg/100 g in ‘Vostorg’ to 784.5 ± 0.3 mg/100 g in ‘Obilnaja’, and TAC ranged from 277.8 ± 1.1 mg/100 g in ‘Čelnočnaja’ to 394.1 ± 8.4 mg/100 g in ‘Nimfa’. Anthocyanins comprised 53.8% of total phenolic contents on average. Among organic acids, citric acid was predominant, averaging 769.41 ± 5.34 mg/100 g, with malic and quinic acids amounting to 289.90 ± 2.64 and 45.00 ± 0.37 mg/100 g on average, respectively. Contents of vitamin C were 34.26 ± 0.25 mg/100 g on average. Organic acids were most effective in the inhibition of both Gram-positive and Gram-negative bacteria tested. In conclusion, berries of L. caerulea are beneficial not only for fresh consumption, but also as a raw material or ingredients of foods with high health-promoting value.

scholarly journals Effect of Adding Resistant Maltodextrin to Pasteurized Orange Juice on Bioactive Compounds and Their Bioaccessibility

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1198
Author(s):  
Elías Arilla ◽  
Purificación García-Segovia ◽  
Javier Martínez-Monzó ◽  
Pilar Codoñer-Franch ◽  
Marta Igual
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Orange Juice ◽  
Control Sample ◽  
Water Soluble ◽  
In Vitro Digestibility ◽  
Total Phenols ◽  
Resistant Maltodextrin

Resistant maltodextrin (RMD) is a water-soluble and fermentable functional fiber. RMD is a satiating prebiotic, reducer of glucose and triglycerides in the blood, and promoter of good gut health, and its addition to food is increasingly frequent. Therefore, it is necessary to study its potential effects on intrinsic bioactive compounds of food and their bioaccessibility. The aim of this study was to evaluate the effect of adding RMD on the bioactive compounds of pasteurized orange juice with and without pulp, and the bioaccessibility of such compounds. RMD was added at different concentrations: 0 (control sample), 2.5%, 5%, and 7.5%. Ascorbic acid (AA) and vitamin C were analyzed using HPLC, whereas total phenols, total carotenoids (TC), and antioxidant capacity were measured using spectrophotometry. After that, sample in vitro digestibility was assessed using the standardized static in vitro digestion method. The control orange juice with pulp presented significantly higher values of bioactive compounds and antioxidant capacity than the control orange juice without pulp (p < 0.05). RMD addition before the juice pasteurization process significantly protected all bioactive compounds, namely total phenols, TC, AA, and vitamin C, as well as the antioxidant capacity (AC) (p < 0.05). Moreover, this bioactive compound protective effect was higher when higher RMD concentrations were added. However, RMD addition improved phenols and vitamin C bioaccessibility but decreased TC and AA bioaccessibility. Therefore, the AC value of samples after gastrointestinal digestion was slightly decreased by RMD addition. Moreover, orange pulp presence decreased total phenols and TC bioaccessibility but increased AA and vitamin C bioaccessibility.

scholarly journals Impact of High-Pressure Homogenization Parameters on Physicochemical Characteristics, Bioactive Compounds Content, and Antioxidant Capacity of Blackcurrant Juice

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1802
Author(s):  
Bartosz Kruszewski ◽  
Katarzyna Zawada ◽  
Piotr Karpiński
Keyword(s):  
High Pressure ◽  
Vitamin C ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Titratable Acidity ◽  
Physicochemical Characteristics ◽  
Food Preservation ◽  
Inlet Temperature ◽  
High Pressure Homogenization

High-pressure homogenization (HPH) is one of the food-processing methods being tested for use in food preservation as an alternative to pasteurization. The effects of the HPH process on food can vary depending on the process parameters used and product characteristics. The study aimed to investigate the effect of pressure, the number of passes, and the inlet temperature of HPH processing on the quality of cloudy blackcurrant juice as an example of food rich in bioactive compounds. For this purpose, the HPH treatment (pressure of 50, 150, and 220 MPa; one, three, and five passes; inlet temperature at 4 and 20 °C) and the pasteurization of the juice were performed. Titratable acidity, pH, turbidity, anthocyanin, vitamin C, and total phenolics content, as well as colour, and antioxidant activity were measured. Heat treatment significantly decreased the quality of the juice. For processing of the juice, the best were the combinations of the following: one pass, the inlet temperature of 4 °C, any of the used pressures (50, 150, and 220 MPa); and one pass, the inlet temperature of 20 °C, and the pressure of 150 MPa. Vitamin C and anthocyanin degradation have been reported during the HPH. The multiple passes of the juice through the machine were only beneficial in increasing the antioxidant capacity but negatively affected the colour stability.

scholarly journals Sebaran Klorofil a, Nitrat, Fosfat dan Plankton Sebagai Indikator Kesuburan Ekosistem Mangrove Tugurejo Semarang

2018 ◽  
Vol 16 (1) ◽  
pp. 68
Author(s):  
Anik Prihatin ◽  
Prabang Setyono ◽  
Sunarto Sunarto
Keyword(s):  
Environmental Factors ◽  
Chlorophyll A ◽  
Aquatic Ecosystems ◽  
Trophic Status ◽  
Nutrient Content ◽  
Mangrove Ecosystem ◽  
Nitrate Content ◽  
Aquatic Biota ◽  
Phosphate Content ◽  
Trophic Index

ABSTRAKEkosistem mangrove merupakan suatu interaksi yang terjadi antara tanaman – tanaman mangrove dengan faktor lingkungan perairan mangrove sehingga dapat menyebabkan terjadinya kesuburan perairan dan sebagai tempat mencari makan alami bagi biota – biota perairan. Tujuan penelitian ini adalah untuk mengetahui sebaran klorofil-a, nitrat dan fosfat serta plankton pada ekosistem mangrove yang berguna untuk kelestarian hidup biota – biota perairan Mangrove. Penelitian ini dilaksanakan bulan Oktober – November 2017 di Mangrove Tapak Tugurejo Semarang. Hasil penelitian ini didapatkan kandungan klorofil a rata – rata 0,165 mg/l, kandungan nitrat rata – rata 2,188 mg/l dan kandungan fosfat rata – rata 0,045 mg/l. Kelimpahan fitoplankton ditemukan sebanyak 23 spesies dan zooplankton ditemukan sebanyak 5 spesies. Berdasarkan status indeks trofik perairan Mangrove Tapak Tugurejo Semarang termasuk dalam kategori perairan mesotrofik, yakni unsur hara dan nutrien dalam perairan mangrove kurang tersedia banyak atau sedang dan belum tercemar. Kondisi kesuburan ekosistem mangrove terpantau cukup baik bila dibandingkan dengan kandungan – kandungan unsur hara, klorofil a dan plankton yang melimpah di perairan penelitian, sehingga memungkinkan banyak terdapat kehidupan biota – bioata perairan. Kesimpulan dari penelitian ini adalah kandungan unsur hara (nitrat dan fosfat) dan klorofil a serta plankton diperairan mangrove sangat mempengaruhi kesuburan ekosistem perairan, serta plankton yang mendominasi tempat penelitian ialah dari kelas Baccilariophyceae sebanyak 21 spesies.Kata kunci: Status trofik, Plankton, Ekosistem Mangrove,  Mangrove Tapak Tugurejo Semarang.ABSTRACTMangrove ecosystem is an interaction that occurs between mangrove plants with environmental factors of mangrove waters that can cause the occurrence of water fertility and as a place of natural foraging for aquatic biota. The purpose of this research is to know the distribution of chlorophyll a, nitrate and phosphate and plankton in mangrove ecosystem which is useful for the preservation of biota life of Mangrove waters. This research was conducted in October - November 2017 at Tapak Mangrove Tugurejo Semarang. The results of this study obtained an average chlorophyll-1 content of 0.165 mg / l, an average nitrate content of 2.188 mg / l and an average phosphate content of 0.045 mg / l. Abundance of phytoplankton found as many as 23 species and zooplankton found as many as 5 species. Based on the trophic index status of Mangrove waters Tapak Tugurejo Semarang included in the category of mesotrophic waters, the nutrients and nutrients in the mangrove waters are less available or moderate and not contaminated. The condition of mangrove ecosystem fertility is observed quite well when compared with nutrient content, chlorophyll-a and plankton abundant in research waters, thus allowing many life biota - bioata waters. The conclusion of this research is the content of nutrients (nitrate and phosphate) and chlorophyll a and plankton in mangrove waters greatly affect the fertility of aquatic ecosystems, and the plankton that dominate the research site is from the Baccilariophyceae class of 21 species.Keywords : Trophic Status, Plankton, Mangrove Ecosystem, Mangrove Tapak Tugurejo SemarangCitation: Prihatin, A, Setyono, P dan Sunarto (2018). Sebaran Klorofil-a, Nitrat, Fosfat dan Plankton Sebagai Indikator Kesuburan Ekosistem di Mangrove Tapak Tugurejo Semarang. Jurnal Ilmu Lingkungan, 16(1), 68-77, doi:10.14710/jil.16.1.68-77

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