scholarly journals Cytotoxic and Genotoxic Effects of Methanol Extracts of Some Gypsophila L. Species

Caryologia ◽  
2021 ◽  
Author(s):  
Ferhan Korkmaz ◽  
Özgün Tuna Gülören ◽  
Meltem Erdir ◽  
Ebru Ataşlar
Keyword(s):  
Mitotic Index ◽  
Chromosome Aberration ◽  
Plant Extracts ◽  
Test Method ◽  
Control Group ◽  
Root Tips ◽  
Genotoxic Effects ◽  
Allium Test ◽  
Methanol Extracts ◽  
Extract Concentration

In this study, the cytotoxic and genotoxic effects of Gypsophila perfoliata L. var. perfoliata, Gypsophila perfoliata L. var. araratica Kit Tan, Gypsophila pilosa Hudson and Gypsophila osmangaziensis Ataşlar & Ocak plant extracts have been examined by using Allium Test method. Methanol extracts of plants have been prepared in 4 different concentrations (0.625 mg/ml, 1.250 mg/ml, 2.500 mg/ml and 5.000 mg/ml). After the onion roots were treated at these concentrations of plant extracts for 24 hours and 48 hours, mitosis preparations were prepared from these root tips. With the data being obtained by evaluating the preparations, mitotic index (%) and  chromosome aberration (%) values have been calculated. Distilled water has been used as control group. It was found out that mitotic index and chromosome aberration values of all species showed significant differences compared to control group in the extract concentration range of 1.250–5.000 mg/ml. It has been determined that the most widely observed chromosome aberrations were disturbed metaphase, sticky metaphase, c-metaphase, disturbed anaphase and anaphase bridge.

scholarly journals Possible protective effects of trans-resveratrol against 1,4-dioxane  induced toxicity in meristematic cells of plant bioassays

2020 ◽  
Author(s):  
Deniz Kurt ◽  
Emine Yalçın ◽  
Kültiğin Çavuşoğlu
Keyword(s):  
Mitotic Index ◽  
Allium Cepa ◽  
Chromosomal Abnormalities ◽  
Treated Group ◽  
Control Group ◽  
Wall Thickening ◽  
Protective Effects ◽  
Allium Test ◽  
Meristematic Cells ◽  
Dose Dependent

Abstract Background In this study, the protective effects of trans-resveratrol against 1,4-dioxane induced toxicity in meristematic cells were investigated. For this purpose, Allium test which is a reliable test was used and the alterations in all experimental groups were examined by using physiological, cytogenetic, biochemical and anatomical parameters.Results As a result of the analysis, it has been determined that 1,4-dioxane causes serious abnormalities in Allium cepa meristematic cells. It was determined that in 1,4-dioxane treated group germination percentage regressed 1.6-times, root length reduced 12.7-times and weight gain decreased 7.7-times compared to control group. It has been observed that trans-resveratrol administration with 1,4-dioxane causes improvement in physiological parameters and reduces the damage rate from 0.4 to 0.16. Mitotic index, micronucleus and chromosomal abnormality frequency were investigated as cytogenetic parameters. It was determined that mitotic index decreased, chromosomal abnormalities and micronucleus frequency increased in 1,4-dioxane treated group. Trans-resveratrol treatment was found to cause a dose dependent improvement in genotoxic effects induced by 1,4-dioxane. Changes in the antioxidant system in all experimental groups were determined by measuring malondialdehyde, glutathione, superoxide dismutase and catalase enzyme levels. It was determined that 1,4-dioxane administration caused an increase in malondialdehyde level, decreased glutathion rate and induced antioxidant enzyme activity. Trans-resveratrol application was found to cause improvement in these alterations induced by 1,4-dioxane. It was observed that the 200 mg/mL trans-resveratrol+1,4-dioxane treatment caused a 1.9-fold decrease in malondialdehyde level which is indicator of lipid peroxidation compared to only 1,4-dioxane treated group. The abnormalities caused by 1,4-dioxane application in the meristematic cells are also found in the anatomical structure. In 1,4-dioxane treatment group, anatomical changes such as cell deformation and cortex wall thickening were observed. The frequency of these changes decreased with trans-resveratrol administration.Conclusions As a result, it was determined that 1,4-dioxane caused a versatile toxicity in Allium cepa meristematic cells, while trans-resveratrol was found to have a dose-dependent protective feature against 1,4-dioxane-induced toxicity.

scholarly journals Assessment of ecotoxical state of technologically modified edaphotopes with waste of oil refinery with the Allium-test method

2021 ◽  
pp. 84-93
Author(s):  
H. Klepach ◽  
◽  
N. Holub ◽  
O. Lupak ◽  
◽  
...  
Keyword(s):  
Apical Meristem ◽  
Micronucleus Test ◽  
Background Level ◽  
Test Method ◽  
Oil Refinery ◽  
Aqueous Extracts ◽  
Genotoxic Effects ◽  
Allium Test ◽  
Research Areas ◽  
High Level

The ecotoxic state of technologically modified edaphotopes by waste of refinery was investigated with the Allium-test method. Material for the investigations was samples of edaphotopes which were selected at a depth of 15–20 cm from seven research areas of different locations. The last ones were 10–200 m away in relation to the surface storages of oil sludge and other waste of refinery. It has been detected in Allium-test that edaphotopes of the research areas located at a distance of 10–50 m relative to the storages of waste are toxic. Particularly, their aqueous extracts cause a medium and high level of phyto-, cyto- and genotoxic effects on the growing and proliferative activity of Allium cepa Stuttgarter Riesen variety meristem. Test extracts of edaphotopes in growing Allium-test caused darkening of rootlets and blackening of their tips (contrary to the light rootlets with light tips in control). In addition to it the growing activity of A. сepa bulb roots that were grown on the aqueous extracts of edaphotopes was decreased in 1.71–3.5 times depending on the location and measure of remoteness of the research area from the storage of waste of the refinery. Phytotoxicity of aqueous extracts of those edaphotopes regarding to A. сepa is above average and higher levels, and tolerance of onion rootlets to the test extracts decreases up to 29–52 % comparing to control. The mitotic activity of apical meristem of rootlets also decreased. Particularly, mitotic indexes diminished to 51.05–63.57 % (in control – 82.34 %) and cytotoxicity ones were in the range of 22.8–48.0 % that confirms the medial level of cytotoxicity of the investigated edaphotopes. Ana-telophase method and micronucleus test have shown that the chromosome aberrations frequency and appearance of micronuclei in the cells of the apical meristem of the test object A. cepa is higher in 3.34–5.20 times comparing to control. It indicates the average and the high level of genotoxicity of the investigated edaphotopes. The edaphotopes, which are located at the distance of 100–200 m in relation to the surface storages of waste of refinery have a slight phyto-, cyto- and genotoxic effects which are close to the background level.

PENGARUH MODEL PEMBELAJARAN PROBING PROMPTING BERBANTUAN MULTIMEDIA TERHADAP HASIL BELAJAR IPA

2018 ◽  
Vol 2 (3) ◽  
pp. 336
Author(s):  
Ni Pt Rasni Karwati ◽  
Km Ngurah Wiyasa ◽  
I Kt Ardana
Keyword(s):  
Elementary School ◽  
Elementary Schools ◽  
Science Learning ◽  
Learning Model ◽  
Test Method ◽  
Fifth Grade ◽  
Control Group ◽  
Fifth Grade Students ◽  
School Year ◽  
Significant Difference

This research aims to determine the significance of the difference in science learning results between the group of fifth-grade students in Gugus I Elementary Schools, North Kuta District, in the school year of 2017/2018, that take lessons with the multimedia-assisted probing-prompting learning model and the group of students that take lessons with the conventional learning. The design of this research is a quasi-experimental research with the nonequivalent control group design. The population of this research are all the fifth-grade students of Gugus I Elementary Schools in North Kuta District that still implement the KTSP, which consists of 10 classes with a total of 339 students. The sampling is conducted using the random sampling technique. The sample in this research are the students of class VB in SD (Elementary School) No.7 Dalung, with 36 students as the experiment group and the students of class VB in SD No.4 Dalung with 28 students as the control group. The data collection is conducted using the test method in the form of the multiple choice objective test. The science learning results are analyzed using the t-test. Based on the average the experiment groups =80,89 > the control group =72,85, which means that the multimedia-assisted probing-prompting learning model has an influence on the science learning result. Based on the hypothesis test, tvalues =4,517> ttable =2,000, with dk=62 and a significance level of 5%. Based on the test criteria, H0 is rejected and Ha is accepted. Thus, it can be interpreted there is a significant difference the science learning result between the group of students that were taught using the multimedia-assisted probing-prompting learning model and the students that were taught using the conventional learning. It can be concluded that the the multimedia-assisted probing-prompting learning model has an influence on the science learning result of the fifth-grade students in Gugus I Elementary School, North Kuta District, in the school year of 2017/2018. Keywords : probing prompting, multimedia, science learning result

Evaluation of genotoxicity of pan masala employing chromosomal aberration and micronucleus assay in bone marrow cells of the mice

2009 ◽  
Vol 25 (7) ◽  
pp. 467-471 ◽  
Author(s):  
BN Mojidra ◽  
K. Archana ◽  
AK Gautam ◽  
Y. Verma ◽  
BC Lakkad ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Chromosomal Aberration ◽  
Bone Marrow Cells ◽  
Micronucleus Assay ◽  
Control Group ◽  
Genotoxic Potential ◽  
Polychromatic Erythrocytes ◽  
Significant Difference ◽  
Marrow Cells

Pan masala is commonly consumed in south-east Asian and other oriental countries as an alternate of tobacco chewing and smoking. Genotoxic potential of pan masala (pan masala plain and pan masala with tobacco known as gutkha) was evaluated employing chromosome aberration (CA) and micronucleus (MN) assay in vivo. Animals were exposed to three different doses (0.5%, 1.5% and 3%) of pan masala plain (PMP) and gutkha (PMT) through feed for a period of 6 months and micronucleus and chromosomal aberrations were studied in the bone marrow cells. Induction of mean micronuclei in polychromatic erythrocytes (MNPCE) and normochromatic erythrocyte (MNNCE) was higher in both types of pan masala treated groups with respect to control group. Both pan masala plain and gutkha treatment significantly induced the frequency of MNPCE and MNNCE in the bone marrow cells, indicating the genotoxic potential. Furthermore, slight decline in the ratio of polychromatic erythrocytes to normochromatic erythrocytes was also noticed, suggesting the cytotoxic potential even though the ratio was statistically non significant. A dose-dependent, significant increase in chromosome aberration was observed in both types of pan masala treated mice with respect to control. However, no significant difference in micronucleus and chromosomal aberration induction was noticed between two types of pan masala exposed (PMP and PMT) groups. Results suggest that both types of pan masala, i.e. plain and gutkha, have genotoxic potential.

EFFECT OF PVMC DEADWOOD AND OF PVMC LACTATION ON THE COW REPRODUCTION

2021 ◽  
pp. 22-27
Author(s):  
О.В. ЛАТЫШЕВА ◽  
А.В. ИВАНОВ
Keyword(s):  
Essential Oils ◽  
Plant Extracts ◽  
Average Duration ◽  
Reproductive Function ◽  
Feed Additive ◽  
Control Group ◽  
Economic Experiment ◽  
Service Period ◽  
Experimental Group

Изучено влияние на репродуктивную функцию коров белково-витаминно-минеральных концентратов (БВМК) с кормовой добавкой «AMG-COMPLEX», состоящей из эфирных масел и растительных экстрактов. Для проведения опыта были сформированы контрольная и опытная группы по 70 голов с учетом количества дней до отела из коров второй и третьей стельности. Животным контрольной группы скармливали основной рацион. Для коров опытной группы были составлены рационы с БВМК, которыми заменяли пропорциональное количество концентрированных кормов и часть витаминно-минерального премикса. Они получали БВМК для сухостойных коров в количестве 300 г на 1 голову в сутки с 60-го по 20-й день до отела и по 200 г на 1 голову в сутки с 20-го дня до отела. После отела 60 дней им скармливали БВМК для раздоя в количестве 1200 г на 1 голову в сутки. Использование БВМК в рационах коров опытной группы способствовало улучшению у них репродуктивной функции и снижению частоты гинекологических заболеваний после отела в 4,5 раза. Индекс осеменения в опытной группе составил 1,73 (P≤0,05), что на 0,76 ниже, чем у животных контрольной группы. Средняя продолжительность сервис-периода в опытной группе составила 87,71 дня (P≤0,01) и была короче, чем в контроле, на 14,72 дня. The effect on the reproductive function of cows of the protein-vitamin-mineral concentrate with the feed additive ʺAMG-COMPLEXʺ, consisting of essential oils and plant extracts, was studied. To experiment, taking into account the number of days before calving, control and experimental group of 70 heads each were formed from cows of the second and third pregnancy. In the scientific and economic experiment, the cows of the control group consumed the feed of the main rations. For the cows of the experimental group rations were made with PVMC, which replaced the proportional amount of concentrated feed and a part of the vitamin-mineral premix. They received PVMC deadwood in the amount of 300 g per 1 head per day from 60th to 20th day before calving and 200 g per 1 head per day 20 days before calving. 60 days after calving they received PVMC lactation in the amount of 1200 g per head per day. Feeding PVMC to cows of the experimental group contributed to the improvement of reproductive function and a decrease in the frequency of gynaecological diseases after calving by 4.5 times. The insemination index in the experimental group was 1.73 (P≤0.05), which is 0.76 lower than in the animals in the control group. The average duration of the service period in the experimental group was 87.71 days (P≤0.01) and was shorter than in the control by 14.72 days.

scholarly journals Toxic Effects of Vitex Negundo L. And Polygonum Hydropiper L. Plant Extract Treated Fishes on Rats

2021 ◽  
Vol 49 (1) ◽  
pp. 125-135
Author(s):  
Molly Harun ◽  
Gulroo Begum Sufi ◽  
Gulshan Ara Latifa
Keyword(s):  
Body Weight ◽  
Radiation Dose ◽  
Plant Extracts ◽  
Control Group ◽  
Vitex Negundo ◽  
Histopathological Changes ◽  
Untreated Control Group ◽  
Polygonum Hydropiper ◽  
Treated Fish ◽  
Dried Fish

An observation was made to taste the toxic effect of Vitex negundo L. and Polygonum hydropiper L. (leaf and bark) plants extracts treated fish fed group on rats, Bandicota indica. Six group of rats were fed on two different kinds of plants extracts, one is radiation dose and another untreated control group. These were, the untreated control group rats were feed on normal food such as dried fish and wheat whereas treated rats were given plant extracts and radiation dose treated by dried fish. The experimental rats were fed of 4 ml dose of plant extracts in 70% ethyl alcohol (ratio 1:10) and 4 kGy radiation treated dry fish. The toxic properties of these plant extracts were carried out the vital organs of liver, kidney, stomach, spleens, lungs and muscles on male rats .The lower mammalian species rats were used for preliminary survey their body weight gained and histopathological changes were observed. The experiment was carried out from August 2004 to December 2004. Initially the concentration of rats body weights were found as, control group (C) 43 g/m, Vitex negundo leaf treated fish fed group of rat (VL) 44.8 g/m, Vitex negundo bark treated fish fed group of rat (VB) 44.4 g/m, Polygonum hydropiper leaf treated fish fed group of rat (PL) 46.2 g/m, Polygonum hydropiper bark treated fish fed group of rat (PB) 47.8 g/m and radiation treated fish fed group of rat (R) 47 g/m. After 30 days the weight gain of rats recorded as, group (C) 324.6 g/m, (VL) 357.2 g/m, (VB) 362 g/m, (PL) 342 g/m, (PB) 323.6 g/m and (R) 352 g/m and 90 days after the body weight were group (C) 727.2 g/m, (VL) 781.2 g/m, (VB) 795.8 g/m, (PL) 780.4 g/m, (PB)764.8 g/m and R 789.8 g/m respectively. The present study is, the first attempt to use the rats body weight data found well and most suitable 4ml dose of V. negundo bark treated extracts than leaf treated group and 4 kGy radiation group. Histopathological changes of rats organs test liver was well preserved, kidney-the glomeruli, tubules, blood vessel were showed no significant changes, in the stomach-the mucosa, sub mucosa were well, in the spleen-red and white pulp were showed no significant changes, lung-the alveoli was good and in the muscle-the skeletal muscle were remain unaltered. Bangladesh J. Zool. 49(1): 125-135, 2021

scholarly journals PENGARUH MODEL QUANTUM TEACHING BERBASIS PENDIDIKAN KARAKTER TERHADAP KOMPETENSI PENGETAHUAN IPA SISWA KELAS V SD GUGUS DEWI SARTIKA DENPASAR TIMUR TAHUN AJARAN 2017/2018

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Ni KomangMega Lestari
Keyword(s):  
Character Education ◽  
Hypothesis Test ◽  
Test Method ◽  
Control Group ◽  
Teaching Model ◽  
Control Group Design ◽  
Significance Level ◽  
Model Base ◽  
5Th Grade ◽  
Academic Year

This research aims to know the effect of quantum teaching model base character education towards science competency knowledge in 5th grade students of Gugus Dewi Sartika East Denpasar academic year 2017/2018. This study was an experiment with quasy experiment design using the Non-Equivalent Control Group Design. The population of this research is all 5th grade of Gugus Dewi Sartika East Denpasar amounting to 328 students. Sample were taken by random sampling technique. The sample of this research is 5th A grade in SDN  10 Kesiman amount 42 students as the experiment group and 5th B grade in SDN 3 Kesiman amount 53 student as the control group. The data were collected using the test method in the form of a standard multiple choice objective test. The data were analyzed using descriptive statistic and inferential in shape gain score from science competency knowledge. The result if data analysis obtained mean of gain skor from experiment group 16X"> =0,34 > 16X"> =0,25 control group. From the hypothesis test using t-test while at 5% significance level and df 83 obtained thitung =3,214 > ttabel=2,000. Based on these result can be concluded that quantum teaching model base character education influence of science competency knowledge of 5th grade students SD Gugus Dewi Sartika East Denpasar academic year 2017/2018.

scholarly journals Pengaruh Model Pembelajaran Reciprocal Teaching Berbasis Penilaian Kinerja Terhadap Kompetensi Pengetahuan IPA

2018 ◽  
Vol 2 (3) ◽  
pp. 275
Author(s):  
Ni ketut Feby puspita ◽  
I wayan Darsana ◽  
I b g s Abadi
Keyword(s):  
Elementary School ◽  
Fourth Grade ◽  
Test Method ◽  
Reciprocal Teaching ◽  
Control Group ◽  
Science Knowledge ◽  
Control Group Design ◽  
Model Based ◽  
Fourth Grade Students ◽  
Experimental Group

This study aimed to know about the affect of reciprocal teaching model based on performance assessment againts the competence science knowledge of fourth grade students of elementary school Gugus I Gianyar district in 2017/2018. This research type is quasi experiment research with nonequivalent control group design. The population of this study is all students of class IV elementary school Gugus I Gianyar district which 440 students. Sample were taken by random sampling technique, so that the IVA class in elementary school 7 Gianyar as the experimental group and the IVB class in elementary school 6 Gianyar as the control group with the total students in each group of 30 students. The data collection was done by the test method, the type of test was the usual multiple-choice objective test. The data obtained were analyzed using t-test analysis of separated variance. The result of data analysis is tcount = 7.067> ttable = 2,000 at 5% significance and dk = 58, then H0 is rejected and Ha accepted. The average value of science knowledge competence of the experimental group X ̅ = 0,46 , while average science knowledge competence of control group X ̅ = 0,26. Based on the results of this research, it can be concluded that the Reciprocal Teaching learning model based on performance assesment affect the knowledge competence of science students fourth grade students of elementary school Gugus I Gianyar districts year 2017/2018

scholarly journals The Effectiveness of Three Different Plant Extracts Used as Irrigant in Removal of Smear Layer: A Scanning Electron Microscope Study

2015 ◽  
Vol 9 (1) ◽  
pp. 16-22 ◽  
Author(s):  
A Gupta ◽  
V Goyal ◽  
J Duhan ◽  
S Hans ◽  
P Sangwan
Keyword(s):  
Plant Extracts ◽  
Root Canal ◽  
Smear Layer ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Syzygium Aromaticum ◽  
Distilled Water ◽  
Scanning Electron Microscope Study ◽  
Layer Removal ◽  
Scanning Electron

ABSTRACT Aim In the present study, the role of three plant extracts as irrigant in root canal cleaning after instrumentation was evaluated. The effect of Syzygium aromaticum (S. Aromaticum), Ocimum sanctum (O. Sanctum) and Cinnamomum zeylanicum (C. zeylanicum) plant extracts was evaluated in smear layer removal. Methods The study was divided into different groups having 5 teeth each using various irrigating agents to evaluate smear layer removal. Group A: O. Sanctum extract; group A1: O. Sanctum extract with EDTA, group B: S. Aromaticum extract; group B1 S. Aromaticum extract with EDTA, group C: C. zeylanicum extract; group C1 C. zeylanicum extract with EDTA and two control group of 5 teeth each in group D: 3% NaOCl; group D1 3% NaOCl with EDTA (as positive control) and group E: Distilled water (as negative control); group E1 3% Distilled water with EDTA. Each tooth was split longitudinally and prepared for examination by scanning electron microscopy. Results The herbal extracts were effective in cleaning root canal walls when combine with EDTA with maximum activity of S. Aromaticum extract with EDTA group. Conclusion Under the condition of present study the three herbal plant extracts were ineffective in removal of smear layer when used alone.

scholarly journals Pengaruh E-Modul Mata Pelajaran Video Editing Berbasis Model Pembelajaran Project based Learning Terhadap Hasil Belajar Siswa SMK

2018 ◽  
Vol 7 (2) ◽  
pp. 176
Author(s):  
Wahyu Restu Aji . ◽  
Dr. Dewa Gede Hendra Divayana, S.Kom., M . ◽  
Dr. Ketut Agustini, S.Si, M.Si. .
Keyword(s):  
Positive Response ◽  
Learning Model ◽  
Project Based Learning ◽  
Test Method ◽  
Control Group ◽  
Video Editing ◽  
Learning Method ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Tujuan penelitian ini untuk mengetahui (1) pengaruh penerapan E-Modul mata pelajaran Video Editing berbasis model pembelajaran Project Based Learning terhadap hasil belajar siswa kelas XI Multimedia di SMK TI Bali Global Singaraja, (2) Untuk mengetahui respon siswa kelas XI Multimedia di SMK TI Bali Global Singaraja terhadap pengaruh penerapan E-Modul mata pelajaran Video Editing berbasis model pembelajaran Project Based Learning. Jenis penelitian ini adalah eksperimen semu dengan rancangan Post Test Only Control Group Design. Pengumpulan data dilakukan dengan metode tes pilihan ganda untuk mengukur ranah kognitif. Data hasil belajar dianalisis melalui uji prasyarat yaitu uji normalitas dan homogenitas dengan hasil 2 kelompok berdistribusi normal dan homogen, dilanjutkan dengan uji hipotesis yang berarti terdapat pengaruh yang signifikan dalam penggunaan E-Modul mata pelajaran Video Editing berbasis model pembelajaran Project Based Learning terhadap hasil belajar siswa. Sedangkan untuk respon menggunakan metode angket. Hasil analisis angket E-Modul mata pelajaran Video Editing berbasis model pembelajaran Project Based Learning diketahui 67% respon sangat positif, 33% respon positif. Kata Kunci : E-Modul, Project Based Learning, hasil belajar, dan respon siswa The objectives of this study is to find out (1) the effect of applying e-model on video editing lesson based Project Based Learning method on XI grade of Multimedia at SMK TI Global Singaraja. (2) The students' respondent on the XI grade of Multimedia at SMK TI Global Singaraja toward the affect of e-model video editing lesson based project based learning method. This study is quasi experimental with used Post Test Only Control Group Design. Data collection was done using Objective Test Method to measure the cognitive domain. The result of the data was analyzed through prerequisite test which is normality and homogeneity test with the result 2 groups normally distributed and homogeneous, continued with hypothesis test which means there was a significant effect between using e-model video editing lesson based Project Based Learning model towards the students' learning result. While for the respondent was using questionnaire method. The analysis result of e-modul video editing lesson based Project Based Learning model questionnaire is known 67% on very positive response, 33% on positive response. keyword : E-Module, Project Based Learning, learning outcomes, students’ respond

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