scholarly journals PEMANFAATAN TEPUNG PACI-PACI (Leucas lavandulaefolia) UNTUK MENGOBATI INFEKSI Aeromonas hydrophila PADA IKAN PATIN (Pangasius sp.)

2018 ◽  
Vol 6 (2) ◽  
pp. 165-176
Author(s):  
Menik Sri Rejeki ◽  
Ade Dwi Sasanti ◽  
Ferdinand Hukama Taqwa
Keyword(s):  
Clinical Feature ◽  
Natural Material ◽  
In Vitro Test ◽  
In Vivo Test ◽  
Randomized Design ◽  
Commercial Feed ◽  
Absolute Growth ◽  
Absolute Length

ABSTRACTMotile Aeromonas Septicaemia (MAS) is one of bacterial A. hydrophila disease frequently infecting freshwater fish include catfish (Pangasius sp.). Controling the disease of bacterial use chemical antibiotic will cause environment pollution. The aim of this research was to determine utilization of natural material that contain antibacterial such Leucas lavandulaefolia powder as antibacterial to treat of A. hydrophila infection for catfish. The implementation of research was from September until October 2014 at the Laboratorium Budidaya Perairan, Faculty of Agriculture, Sriwijaya University and Laboratorium UPT Klinik Kesehatan Sriwijaya University. Research method was using Completely Randomized Design (CRD) with five treat and three replications. The catfish was infected by A. hydrophila with clinical feature were (inflamed and hemorrhage) that give addition Leucas lavandulaefolia powder on commercial feed as many as 4%, 6%, 8%, and 10%. The result showed that addition 10 % of Leucas lavandulaefolia powder on commercial feed was the best to treat of A. hydrophila infection for catfish were significant (P<0,05). In vitro test result showed that Leucas lavandulaefolia powder  at a concentration 10% as an antibacterial potential of A.hydrophila with inhibitor zone diameter 0.5 cm, at in vivo test the catfish that gives addition Leucas lavandulaefolia powder on commercial pellet as many as 10% capable hematocrit increase, reducing leucocyte completely, produce recovery percentage 84.44%, the survival rate 76.67%, highest absolute growth of catfish 3.03 g and higest absolute length of catfish 0.89 cm. Keywords : A. hydrophila, catfish, Leucas lavandulaefolia powder

scholarly journals EFEKTIVITAS SENYAWA NONATSIRI DARI Curcuma spp. TERHADAP PENEKANAN PENYAKIT ANTRAKNOSA PADA BUAH CABAI

2020 ◽  
Vol 31 (1) ◽  
pp. 21
Author(s):  
Anella Retna Kumala Sari ◽  
Firdaus Auliya Rahmah ◽  
Syamsuddin Djauhari
Keyword(s):  
Curcuma Longa ◽  
Fungal Growth ◽  
In Vitro Test ◽  
Test Results ◽  
In Vivo Test ◽  
Randomized Design ◽  
Vitro Test ◽  
Rotary Vacuum

<em></em><em>One of the important diseases on chili is anthracnose caused by </em>Colletotrichum capsici<em>. </em>Curcuma<em> extracts and </em><em>their essential oils were known as antifungal, but nonessential compounds have not been widely tested. This study aimed to assay the effectiveness of nonessential compounds of </em>Curcuma longa<em>, </em>C. zedoaria<em>, and </em>C. aeruginosa<em> to </em>C. annuum<em>. This study was conducted in November 2014 until Mei 2015 at Brawijaya University. The nonessential compound was obtained by soaking rhizome of </em>C. longa,   C.   zedoaria<em>,  and </em>C.   aeruginosa<em> in methanol, then distilled by</em><em>using rotary vacuum evaporator. Nonessential chemical compunds were identified by using HPLC. Effectiveness evaluation of nonessential compounds from three species of </em>Curcuma<em> was done by in </em>vitro<em> and </em>in vivo<em> test. Tested treatments were three species of </em>Curcuma<em> spp and 6 concentration levels of nonessential compounds (0 ppm, 4 ppm, 6 ppm, 8 ppm, 10 ppm, and</em><em> 12 ppm). The xperiment was performed in Factorial Complete Randomized Design, with 18 treatments combination, and replicated three times. Results of HPLC analysis showed the rhizomes of the three </em>Curcuma<em> species contained curcumin and </em>desmethoxycurcumin<em> in various concentrations. The highest </em><em>level was found in the </em>C. longa<em> extract (13.792 ppm curcumin and 67.156 ppm </em>desmethoxycurcumin<em>). However, in vitro test results showed nonessential compound of </em>C. zedoaria<em> was most effective in inhibiting </em>C. annuum<em> growth.  The 10 ppm concentration inhibited 81.53 % of fungal growth.  Further, the in vivo test, also indicated the same, it’s most effective in hampering the growth of anthracnose symptoms. Therefore, curcumin and </em>desmethoxycurcumin<em> from three species of </em>Curcuma<em> have potential to be developed as botanical fungicide.</em>

scholarly journals Sensitivity Testing of Ciplukan (Physalis angulata) Rod Crude Extract on The Bacterium Pseudomonas fluorescens In Vitro

2019 ◽  
Vol 10 (1) ◽  
pp. 24-30
Author(s):  
Ion Tarsardo Sianturi ◽  
Arief Prajitno ◽  
Ellana Sanoesi
Keyword(s):  
Experimental Method ◽  
Crude Extract ◽  
Inhibition Zone ◽  
Natural Material ◽  
Sensitivity Testing ◽  
Randomized Design ◽  
Physalis Angulata ◽  
Completely Randomized Design

Diseases is a problem in aquaculture, one of which is the diseases caused by the bacteriumP.fluorescens. Antibiotics and chemicals materials themselves can give the bacterium resistance and cause harm for the environment. One alternative that can be done is using a natural material, namely the rod of ciplukan (p. angulata). The purpose of this research was to explain the effect of P. angulata on the growth of p. fluorescens. The method which can be use is experimental method by using the research design of completely randomized design (ral) with 5 treatment and 2 control with 3 repetitions. The results showed that extract of P. angulata at various dose (6,67 ppt, 13,33 ppt, 19,99 ppt, 26,66 ppt and 33,33 ppt) exhibited anti-P. fluorescens activity with inhibition zone diameters in the range of (4,06±0,07 mm - 9,63±1,61 mm). Increashing extract dose lead to increased the inhibition zone. The extract dose of 33,33 ppt exhibited best anti-P. fluorescens activity in this research. The research results show that P. angulata is proven to be able to inhibit the P. fluorescens, but to prove the effectiveness of this material, an in vivo is required.

scholarly journals PRODUCTION AND FUNGICIDAL ACTIVITY ASSESMENT OF WOOD-WASTE LIQUID SMOKE

2020 ◽  
Vol 8 (10) ◽  
pp. 285-291
Author(s):  
Budy Rahmat ◽  
Dedi Natawijaya ◽  
Endang Surahman
Keyword(s):  
Plant Pathogens ◽  
Pathogenic Fungus ◽  
Block Design ◽  
Control Plant ◽  
Wood Waste ◽  
In Vitro Test ◽  
In Vivo Test ◽  
Liquid Smoke

Liquid smoke is known to contain compounds that can control plant disease pathogens. This study aims to produce wood-waste liquid smoke and determine its effectiveness as a fungicide on plant pathogens. This research was conducted in two experimental stages, namely: (i) in vitro test as a preliminary test of the effectiveness of teak waste liquid smoke at concentrations of 0, 0.5, 1, 1.5, 2, and 2.5%; and (ii) in vivo test was arranged in randomized block design consisting of seven levels of liquid smoke concentration, namely 0, 1, 2, 3, 4, 5, and 6%, each of which was repeated four times. The results showed that the pyrolysis of 1 kg of wood waste was produced with the proportions of liquid smoke, charcoal and tar, respectively: 312 mL, 31 g, 367 g and the uncondensed gases. Treatment of liquid smoke in the in vivo test showed that a concentration of 1 to 2.5% liquid smoke was able to suppress the growth of the pathogenic fungus Sclerotium rolfsii 100%. The treatment of liquid smoke in the in vivo test showed an effect on inhibition of the growth diameter of fungal colonies, suppressing the disease occurance, and suppressing the lesion diameter.

scholarly journals Triticum aestivum in open skin wounds: cytotoxicity and collagen histopathology

2018 ◽  
Vol 39 (4) ◽  
pp. 1547
Author(s):  
Mariana Teixeira Tillmann ◽  
Cláudia Beatriz De Mello Mendes ◽  
Geferson Fischer ◽  
Antonio Sergio Varela Júnior ◽  
Cristina Gevehr Fernandes ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Cell ◽  
Negative Control ◽  
In Vitro Test ◽  
Skin Wounds ◽  
In Vivo Test ◽  
Collagen Formation

Phytoterapic compounds have been used in wound healing for many centuries. Nowadays, scientific evidences of phytotherapeutics is a requirement of the legislation. The scientific literature notes the need for healing topics yielding scars that are both aesthetically appealing and resistant. We aimed to evaluate the cytotoxicity of several doses of T. aestivum extract (2 mg mL-1, 4 mg mL-1, 6 mg mL-1, 8 mg mL-1 and 10 mg mL-1) in a fibroblast cell line and the healing process in an in vivo experimental model (New Zealand rabbits). For this, MTT test in 3T6 cells was performed in duplicates using MEM (0 mg ml-1) as negative control. Cell viability was calculated as: absorbance average in treatments/absorbance average in controls x 100. In vivo test was performed in 78 skin wounds in rabbits that were treated with 2 mg ml-1and 10 mg ml-1 of T. aestivum and non-ionic cream for 21 days. After this period, it was evaluated the histology using picrosorius and Gomori’s trichrome staining. Statistical analysis was evaluated using T test (Graphpad) for cytotoxicity assay, Fischer test for the gomori trichrome test (Grahpad) and Kruskal-Wallis (Statistic 9.0) for picrosirius test. The in vitro test resulted in cytotoxicity observed at 2mg mL-1 whereas cells were viable at higher doses. On the other hand, it was observed that collagen formation of wounds was more uniform with this dose than with 10mg mL-1 extract in the in vivo study. Thus, we conclude that the 2mg mL-1 T. aestivum aqueous extract dose was more efficient in the in vivo wound healing study, despite its cytotoxic effects in vitro.

scholarly journals Efektivitas Antifungi Ekstrak Curcuma aeruginosa terhadap Patogenisitas Colletotrichum capsici pada Tanaman Cabai Merah (Antifungi Effectivity of Curcuma aeruginosa Extract to Colletotrichum capsici Pathogenicity on Red Chilli Pepper)

2021 ◽  
Vol 30 (2) ◽  
pp. 141
Author(s):  
Anella Retna Kumala Sari ◽  
Arrohmatus Syafaqoh Li’aini
Keyword(s):  
Chilli Pepper ◽  
In Vivo Test ◽  
Colletotrichum Capsici ◽  
Randomized Design ◽  
Pepper Fruit ◽  
Time Utilization ◽  
Group Play ◽  
Rotary Vacuum

<p>Antraknosa yang disebabkan oleh Colletotrichum capsici masih menjadi penyakit utama yang menyerang buah cabai merah. Selama ini, penggunaan Curcuma aeruginosa sebagai antimikrobe patogen penyebab penyakit pada manusia lebih populer daripada sebagai antimikrobe fitopatogen. Rimpang Curcuma diketahui mengandung senyawa volatil/atsiri dan nonvolatil. Potensi senyawa volatil/atsiri Curcuma sebagai antimikrobe telah banyak dilaporkan, namun masih sangat terbatas untuk senyawa nonvolatilnya. Penelitian ini bertujuan mengetahui efektivitas antifungi senyawa nonvolatil dari ekstrak C. aeruginosa terhadap C. capsici pada buah cabai merah. Penelitian dilaksanakan pada bulan November 2014 hingga Mei 2015 di Universitas Brawijaya. Senyawa nonvolatil didapatkan dengan merendam rimpang C. aeruginosa menggunakan pelarut metanol kemudian didistilasi menggunakan rotary vacuum evaporator dan diidentifikasi menggunakan HPLC. Efektivitas antifungi senyawa nonvolatil dari ekstrak rimpang C. aeruginosa diuji secara in vitro dan in vivo di laboratorium menggunakan Rancangan Acak Lengkap dengan tiga kali ulangan. Perlakuan yang diujikan yaitu konsentrasi senyawa nonvolatil terdiri atas 0 (kontrol), 4, 6, 8, 10, dan 12 ppm. Senyawa nonvolatil dari ekstrak rimpang C. aeruginosa sangat efektif menghambat pertumbuhan C. capsici baik secara in vivo maupun in vitro di laboratorium bahkan konsentrasi 12 ppm menunjukkan persentase penghambatan antraknosa hingga 100%. Hasil identifikasi HPLC menunjukkan bahwa senyawa nonvolatil dari ekstrak rimpang C. aeruginosa mengandung kelompok Curcuminoid yang terdiri atas curcumin dan demethoxycurcumin yang berperan sebagai antifungi sehingga sangat berpotensi dikembangkan sebagai fungisida nabati.</p><p><strong>Keywords</strong></p><p>Antraknosa; Cabai merah; C. aeruginosa; Senyawa nonvolatil</p><p><strong>Abstract</strong></p><p>Anthracnose caused  by Colletotrichum capsici  is  still  as  major disease against  chilli pepper fruit. During this time, utilization of C. aeruginosa as pathogen antimicrobial caused disease to human is more popular than to crops. Curcuma has been known containing volatile and nonvolatile compound. Potential of volatil compound from Curcuma as antipathogen has been reported widely, nevertheless it is still limited known for nonvolatile compound. This research aimed to understand the antifungi effectivity of nonvolatile compound from C. aeruginosa extract to C. capsici on chilli pepper fruit. Research was conducted in November 2014 to Mei 2015. Nonvolatile compound was obtained by soaking C. aeruginosa rhizome into methanol solvent then distilated using rotary vacuum evaporator  and identified with HPLC instrument. Antifungi  effectivity  of nonvolatile compound  from  C. aeruginosa  extract  was experimented by in vitro and in vivo test using Completely Randomized Design with three replications. Treatments tested were various concentrations of nonvolatile compound namely 0 (control), 4, 6, 8,10, and 12 ppm. Nonvolatile compound from C. aeruginosa extract was highly effective to inhibit growth of C. capsici by both in vitro and in vivo test. HPLC identification result showed nonvolatile compound from C. aeruginosa extract contains Curcuminoid group play role as antifungi.</p>

scholarly journals Epidemiology of Sheep Lice and Efficacy Evaluation of 60% Diazinon and 1% Ivermectin against Bovicola ovis in Sayint District, South Wollo, Ethiopia

2020 ◽  
Author(s):  
Simegn Legesse ◽  
Mussie Hailemelekot ◽  
Habtamu Tamrat ◽  
Yeshwas Ferede
Keyword(s):  
Drug Efficacy ◽  
In Vitro Test ◽  
Efficacy Evaluation ◽  
Cross Sectional ◽  
Randomized Design ◽  
Present Study Area ◽  
Multistage Sampling ◽  
Associated Risk Factors

Abstract Background Sheep lice, caused by Bovicola ovis are very common in Ethiopia. It can result decreased production and reproduction, downgrading and rejection of skins in tannery industries. A cross-sectional and experimental study were conducted in Sayint District, South Wollo, Ethiopia which aims to determine the prevalence of sheep lice with the associated risk factors, identify the major sheep lice species and evaluate the efficacy of 60% Diazinon and 1% Ivermectin against Bovicola ovis. Study kebeles and animals were selected using purposive and multistage sampling techniques. Accordingly, a total of 232 sheep were sampled to estimate the prevalence of sheep lice. Fifteen sheep for in-vivo and 80 Bovicola ovis lice for in-vitro test were used for drug efficacy trial, by using completely randomized design. Results The overall prevalence of sheep lice in this study was 48%. Hair length (OR = 2, P = 0.00), body condition (OR = 1.9, P = 0.02), agro ecology (OR = 1.19, P = 0.00) and season (OR = 2, P = 0.01) were significantly (P < 0.05) associated with sheep lice infestation. The dominant sheep lice species in the study area were Bovicola ovis with prevalence of 83% and mixed infestation of 17% Bovicola ovis with Linognatus ovillus. In this study, the efficacy of 60% Diazinon and 1% Ivermectin against Bovicola ovis were 97% and 81%, respectively. Conclusion The overall prevalence of sheep lice infestation in the present study area was higher, in which Bovicola ovis was predominant and resistant against 1% Ivermectin. Therefore, tailor made intervention is required with a view to reducing the prevalence of sheep lice infestation and addressing drug resistance in the study area.

scholarly journals In-vitro and In-vivo Determinations of Sun Protection Factors (SPF) of Skin Lotions Containing Mountain Papaya Fruit and Mangosteen Peel Ethanolic Extract

2020 ◽  
Vol 25 (3) ◽  
Author(s):  
Heru Sasongko ◽  
Natasya Advaita ◽  
Ratih Guswinda Lestari ◽  
Karimah Umar Aidid
Keyword(s):  
Sun Exposure ◽  
Sun Protection ◽  
In Vitro Test ◽  
Garcinia Mangostana ◽  
In Vivo Test ◽  
Antioxidants Activity ◽  
Mangosteen Peel ◽  
Vitro Test

Indonesia is a high sun exposure country. Exposure to ultraviolet (UV) causes various kinds of skin disorders such as erythema, sunburn, aging, and cancer. Mountain papaya fruit (Vasconcellea pubescens A.DC.) and mangosteen peel (Garcinia mangostana L.) contains metabolite compounds that can protect the skin from sunlight because of its antioxidants activity. The purpose of this study to determine whether the combination of the mountain papaya fruit and mangosteen peel extracts in skin lotion can be used as sun protectors through the in-vitro and in-vivo study. The experiment was done by extracting the mountain papaya fruit and mangosteen peel through the maceration method. The extracts were formulated into skin lotion in three different formulas with the ratio of mountain papaya fruit extract: mangosteen peel extract as follows F1(1:1), F2(1:3), and F3(3:1). In vitro test was done by using UV-VIS spectrophotometry to determine the SPF value and in vivo test was used erythema-induced rats by exotera beam light. The result of in vitro test gained a high enough SPF value for all three formulas F1=23,23; F2=21,70 and F3=28,64 and the result of in vivo test showed that all three formulas did not indicate the existence of erythema value.         It can be concluded that three skin lotion formulas containing mountain papaya fruit and mangosteen peel ethanol extract have the effect of sun protection.

Alterations in Erythrocyte Morphology Induced by Blood Pumps

1993 ◽  
Vol 16 (9) ◽  
pp. 653-658 ◽  
Author(s):  
P. Calzavara ◽  
S. De Angeli ◽  
A. Nieri ◽  
C. Furlan ◽  
R. Bolzonella ◽  
...  
Keyword(s):  
Toxic Substances ◽  
In Vitro Test ◽  
Significant Drop ◽  
Erythrocyte Morphology ◽  
In Vivo Test ◽  
Significant Difference ◽  
Image Analyser ◽  
Vitro Test

A scanning electron microscopy was used after in vitro and in vivo tests to investigate any alterations caused by the peristaltic roller pump in erythrocyte morphology. The electron micrographs of samples were examined as follows: 1) by image analyser; 2) by applying Bessis's classification for the qualitative study of crenated red blood cells (RBCs). The in vitro test was repeated four times using blood from healthy donors. Each basal blood sample was divided into 250 ml portions, each of which was recirculated for 12 minutes at different flow rates. In order to verify any persistent erythrocyte damage caused by the peristaltic pump, 15 minutes after recirculation at 450 ml/min, another sample was prepared using the blood remaining from the last test. A statistically significant direct correlation was found between blood flow (Qb) increase and the percentage of morphologically altered RBCs, when either using an image analyser (r = 0.97; p < 0.05) or Bessis's classification (r = 0.95; p < 0.05). However, neither method showed any statistically significant difference between the percentage of deformed RBCs, determined in the basal sample, or in the percentage found at the end of the 450 ml/min test after standing 15 minutes at room temperature. The in vivo test was carried out on 6 patients over 2 dialysis sessions, which differed only for the Qb: 250 versus 400 ml/min. The two dialysis sessions gave comparable results when using both study methods regarding the presence of deformed RBCs. While Bessis's classification showed a significant drop in the post-dialysis percentage of dysmorphic RBCs compared to the pre-dialysis value, both with a Qb of 250 ml/min and 400 ml/min, no significant change was found with the image analyser. The contradictory results of the two tests can be attributed to the presence of spherocytes and stomatocytes in the in vivo test which on the other hand were absent in the in vitro test and not easily distinguished by the image analyser with the parameter used. Reduction in the number of deformed RBCs after dialysis in the in vivo test can be attributed to improvement in the acidosis, correction of the hydroelectric imbalances and removal of toxic substances as a result of dialysis, thus allowing the echinocytes, spherocytes and stomatocytes to be transformed into discocytes.

scholarly journals Tanaman Bakau Api-api Putih (Avicenia marina) Berpotensi Menghambat Mikrob Patogen dan Melindungi Post Larva Udang Windu (THE POTENTIAL OF AVICENNIA MARINA TO INHIBITS PATHOGEN MICROBES AND PROTECTS THE POST LARVA OF TIGER PRAWN)

2018 ◽  
Vol 19 (1) ◽  
pp. 45 ◽  
Author(s):  
Gina Saptiani ◽  
Andi Noor Asikin ◽  
Fikri Ardhani ◽  
Esti Handayani Hardi
Keyword(s):  
Sea Water ◽  
Water Extract ◽  
Avicennia Marina ◽  
In Vitro Test ◽  
Pathogen Infection ◽  
In Vivo Test ◽  
Agar Disc ◽  
Tiger Prawn

The research aims to study potency of Avicennia marina leaf extract to inhibit pathogens in vitro and in vivo on post larvae of tiger prawn. The leaves we re chopped, dried and extracted with water solvents, sea water andethanol. In vitro inhibitory tests were performed using agar disc diffusion (ADD) and minimal inhibitory concentration (MIC) methods. The in vivo test on tiger prawn PL-8 was given by submersion, which then tested with Vibrio harveyi and Saprolegnia sp. The in vitro test showed A. marina extract can inhibit V.harveyi and Saprolegnia sp. In vivo tests of V. harveyi and Saprolegnia sp. infections, showed 60- 88% survival and 57.33-86.67%, with protective ability of the highest pathogen infection of 80.80% and 77.80%. The best A. marina extract that can inhibit microbial and protect the tiger prawn from pathogen infection is ethanol extract with concentration 1,250-1,500 ppm, followed by sea water extract 1,500 ppm and water extract 1,500 ppm respectively

scholarly journals Potential of Chromolaena odorata Leaf as A Cure of Aeromonas hydrophila on Giant Gouramy (Osphronemus gouramy)

2007 ◽  
Vol 4 (2) ◽  
pp. 139 ◽  
Author(s):  
Y. Hadiroseyani ◽  
. Hafifuddin ◽  
M. Alifuddin ◽  
H. Supriyadi
Keyword(s):  
Aeromonas Hydrophila ◽  
Leaf Extract ◽  
In Vitro Study ◽  
In Vitro Test ◽  
Chromolaena Odorata ◽  
In Vivo Test ◽  
Treated Fish ◽  
Vitro Test

<p>This study was conducted to examine the potency of <em>Chromolaena odorata</em> leaf extract as a medicine for skin eruption disease caused by  Aeromonas hydrophila in giant gouramy <em>Osphronemus gouramy</em>.  Leaf extract of Chromolaena odorata for in vitro test was 0 (as control), 13000, 15000, 17000, 19000 and 21000 ppm, poured onto TSA medium containing bacteria 103 cfu/ml, and then is incubated for 24 hours. In vivo test was performed by injecting bacteria 0.1 ml of 10<sup>9</sup> cfu/ml intramuscularly into giant gouramy (14 g weight), and then  fish were maintained in the water containing 15000 ppm of Chromolaena odorata leaf extract. In vitro study showed that prevention area of leaf extract against Aeromonas hydrophila was increase by increasing the concentration of leaf extract used, reached 9,33 mm.  Prevention zone of leaf extract by difusion tends to constant, reached 7,6 mm. By in vivo test, survival rate of giant gouramy infected by <em>Aeromonas hydrophila</em> was no significantly different between dosages of leaf extract.  All treated fish, excluded control died after 24 hours infection.</p> <p>Keywords: <em>Aeromonas hydrophila</em>, <em>Osphronemus gouramy</em>, <em>Chromolaena odorata</em></p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini dilakukan untuk mengetahui potensi daun kirinyuh <em>Chromolaena odorata</em> sebagai obat untuk penyakit cacar yang diakibatkan oleh <em>Aeromonas hydrophila</em> pada ikan gurame <em>Osphronemus gouramy</em>. Konsentrasi ekstrak daun <em>Chromolaena odorata</em> untuk uji <em>in vitro</em> adalah 13000, 15000, 17000, 19000 dan 21000 serta 0 ppm sebagai kontrol, yang diletakkan di atas media TSA yang telah mengandung biakan bakteri 103 cfu/ml dan diinkubasi selama 24 jam. Uji <em>in vivo</em> dilakukan dengan menginjeksikan bakteri  sebanyak 0,1 ml (10<sup>9</sup> cfu/ml) secara intramuskular ke ikan gurame (berat 14 g) dan kemudian ikan dipelihara dalam air yang mengandung ekstrak daun kirinyuh 15000 ppm. Hasil uji <em>in virto</em> menunjukkan bahwa semakin tinggi konsentrasi ekstrak daun kirinyuh basah semakin efektif dalam menghambat perkembangan <em>A. hydrophila</em> dengan zona hambat tertinggi mencapai 9,33 mm. Zona hambat yang dihasilkan melalui metode difusi cenderung konstan, mencapai 7,6 mm. Melalui uji <em>in vivo</em>, tingkat kelangsungan hidup ikan gurame yang tidak berbeda nyata pada masing-masing perlakuan, bahkan terjadi kematian total dalam 24 jam pada semua perlakuan, kecuali kontrol.</p> <p>Kata kunci: <em>Aeromonas hydrophila</em>, <em>Osphronemus gouramy</em>, <em>Chromolaena odorata</em></p>

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