scholarly journals Gamma Radiation Synthesis of Silver Nanoparticles/Chitosan composites with antimicrobial properties

2021 ◽  
Vol 57 (4) ◽  
pp. 88-95
Author(s):  
Eduard-Marius Lungulescu ◽  
Radu Setnescu ◽  
Nicoleta-Oana Nicula ◽  
Ioana Ion ◽  
Virgil Marinescu
Keyword(s):  
Silver Nanoparticles ◽  
High Stability ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Spherical Shape ◽  
Silver Ions ◽  
Size Distributions ◽  
Particle Size Distributions ◽  
One Step ◽  
Radiochemical Synthesis

Composites of Silver nanoparticles/chitosan were obtained in aqueous solution, in-one step and eco-friendly synthesis, under ambiental conditions, using gamma irradiation. The radiochemical synthesis enabled obtaining of controlled size, monodisperse and high stability Silver nanoparticles. The obtained composites presented UV-Vis surface plasmon resonance comprised between 406-414 nm, depending on composition of the reactant system, spherical shape and narrow particle size distributions, with mean dimensions between 3-55 nm, and good antibacterial properties proven against Staphylococcus aureus and Escherichia coli. The influence of the Silver ions/chitosan ratio and of the pH of the initial solution on the final Ag Np properties is also discussed.

Characterization and antibacterial properties of Eriobotrya japonica extract loaded silver-nanoparticles

2020 ◽  
Vol 16 ◽  
Author(s):  
Arfaa Sajid ◽  
Qaisar Manzoor ◽  
Anam Sajid ◽  
Muhammad Imran ◽  
Shanza Khalid ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Silver Ions ◽  
Eriobotrya Japonica ◽  
X Ray Diffraction ◽  
Effective Utilization ◽  
E Coli ◽  
Elongated Nanoparticles

Background:: Currently, developing methods for the formation of nanoparticles with antimicrobial properties based on green chemistry are the research hotspots. In this research green biosynthesis of Eriobotrya japonica extract loaded silver nanoparticles and their characterization were the main objectives to achieve. Methods:: Green synthesis of E. japonica leaves extract-loaded silver nanoparticles (AgNPs) was carried out and its effect on bacterial growth was examined. The reduction of silver ions in solution was observed using UV-Vis spectrophotometer. The properties of AgNPs were assessed using Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Also, their antibacterial effects were checked against Staphylococcus aureus and Escherichia coli. Results:: It was revealed that 5-50 nm sized spherical to elongated nanoparticles were synthesized that possessed comparatively better antibacterial potential against E. coli and S. aureus than conventional extract of the E. japonica leaves. Conclusions:: Green synthesis and effective utilization of Eriobotrya japonica extract loaded silver nanoparticles is a promising approach for nanoparticle production avoiding negative environmental impacts.

scholarly journals Antibacterial Effect of Colloidal Suspensions Varying in Silver Nanoparticles and Ions Concentrations

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 31
Author(s):  
Varvara Platania ◽  
Alexandra Kaldeli-Kerou ◽  
Theodora Karamanidou ◽  
Maria Kouki ◽  
Alexander Tsouknidas ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Colloidal Suspensions ◽  
Silver Ions ◽  
Cellular Level ◽  
Bacterial Strains ◽  
Three Samples ◽  
Flow Filtration

A lot of effort has been dedicated recently to provide a better insight into the mechanism of the antibacterial activity of silver nanoparticles (AgNPs) colloidal suspensions and their released silver ionic counterparts. However, there is no consistency regarding whether the antibacterial effect displayed at cellular level originates from the AgNPs or their ionic constitutes. To address this issue, three colloidal suspensions exhibiting different ratios of AgNPs/silver ions were synthesized by a wet chemistry method in conjunction with tangential flow filtration, and were characterized and evaluated for their antimicrobial properties against two gram-negative, Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), and two gram-positive, Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis), bacterial strains. The produced samples contained 25% AgNPs and 75% Ag ions (AgNP_25), 50% AgNPs and 50% Ag ions (AgNP_50), and 100% AgNPs (AgNP_100). The sample AgNP_100 demonstrated the lowest minimum inhibitory concentration values ranging from 4.6 to 15.6 ppm for all four bacterial strains, while all three samples indicated minimum bactericidal concentration (MBC) values ranging from 16.6 ppm to 62.5 ppm against all strains. An increase in silver ions content results in higher bactericidal activity. All three samples were found to lead to a significant morphological damage by disruption of the bacterial cell membranes as analyzed by means of scanning electron microscopy (SEM). The growth kinetics demonstrated that all three samples were able to reduce the bacterial population at a concentration of 3.1 ppm. SEM and growth kinetic data underline that S. epidermidis is the most sensitive among all strains against the investigated samples. Our results showed that all three AgNPs colloidal suspensions exhibited strong antibacterial properties and, thus, they can be applied in medical devices and antimicrobial control systems.

Immobilization of Silver Nanoparticles on Chitosan-Coated Silica-Gel-Beads and the Antibacterial Activity

2021 ◽  
Vol 892 ◽  
pp. 36-42
Author(s):  
Muhammad Iqbal Hidayat ◽  
Muhammad Adlim ◽  
Ilham Maulana ◽  
Muhammad Zulfajri
Keyword(s):  
Silver Nanoparticles ◽  
Silica Gel ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Chitosan Film ◽  
Silver Ions ◽  
Filter Material ◽  
Silver Ion ◽  
Gel Beads ◽  
Metal Colloid

Silver nanoparticles (Ag0) have attracted the most attention due to their broad antimicrobial application and outstanding activity. The silver nanoparticles are usually in colloidal form, then immobilization the colloid onto solid support is still interesting to explore. In this work, a new method for silver colloidal nanoparticle immobilization on silica gel beads (SiG), which was then symbolized as Ag0-[chi-SiG] was conducted and characterized successfully. The finding proved that SiG must be coated with three chitosan film layers to give stable support for silver nanoparticles. This coating method caused the chitosan completely covered SiG, and the chitosan film provides coordination bonding for silver ions. The most appropriate solvent for silver ion impregnation on the surface of chi-SiG is methanol compared to other solvents. Tungsten lamp as the photo-irradiation, which is low cost and environmentally friendly has been proven effective for silver ion reduction, as shown by silver metal colloid UV-Vis surface plasmon resonance at 400-700 nm. Ag0-[chi-SiG] showed the antibacterial properties of inhibiting the growth Staphylococcus aureus and Escherichia coli; then it provides the potential application for antibacterial filter material. According to the weight comparison between antibacterial standard and Ag content, then Ag0-[chi-SiG] has two and five times higher of exhibiting zone for each bacteria.

scholarly journals A REVIEW: A GREEN APPROACH FOR THE SYNTHESIS OF SILVER NANOPARTICLES AND ITS ANTIBACTERIAL APPLICATIONS

2018 ◽  
Vol 11 (8) ◽  
pp. 74 ◽  
Author(s):  
Shyla Marjorie Haqq ◽  
Amit Chattree
Keyword(s):  
Silver Nanoparticles ◽  
Antimicrobial Properties ◽  
Nanoparticle Synthesis ◽  
Silver Ions ◽  
Multidrug Resistant ◽  
X Ray Diffraction ◽  
Green Approach ◽  
Transmission Electron ◽  
Uv Visible ◽  
Silver Nanoparticle Synthesis

  This review is based on the synthesis of silver nanoparticles (AgNPs) using a green approach which is biofabricated from various medicinal plants. AgNPs were prepared from the various parts of the plants such as the flowers, stems, leaves, and fruits. Various physiochemical characterizations were performed using the ultraviolet (UV)-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction spectroscopy, transmission electron microscopy, and energy dispersive spectroscopy. AgNPs were also used to inhibit the growth of bacterial pathogens and were found to be effective against both the Gram-positive and Gram-negative bacteria. For the silver to have antimicrobial properties, it must be present in the ionized form. All the forms of silver-containing compounds with the observed antimicrobial properties are in one way or another source of silver ions. Although the antimicrobial properties of silver have been known, it is thought that the silver atoms bind to the thiol groups in enzymes and subsequently leads to the deactivation of enzymes. For the silver to have antimicrobial properties, it must be present in the ionized form. The study suggested that the action of the AgNPs on the microbial cells resulted into cell lysis and DNA damage. AgNPs have proved their candidature as a potential antibacterial against the multidrug-resistant microbes. The biological agents for synthesizing AgNPs cover compounds produced naturally in microbes and plants. Reaction parameters under which the AgNPs were being synthesized hold prominent impact on their size, shape, and application. Silver nanoparticle synthesis and their application are summarized and critically discussed in this review.

scholarly journals Production and Characterization of Keratin/Tragacanth Gum Nanohydrogels for Drug Delivery in Medical Textiles

2021 ◽  
Vol 8 ◽  
Author(s):  
Nazanin Mansouri Shirazi ◽  
Niloofar Eslahi ◽  
Adeleh Gholipour-Kanani
Keyword(s):  
Disulfide Bonds ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Spherical Shape ◽  
Herbal Extract ◽  
Minimum Size ◽  
Mechanical Resistance ◽  
Tragacanth Gum ◽  
Medical Textiles

Keratin protein has been applied for biomedical applications due to its biocompatibility, biodegradability, mechanical resistance, and bioavailability. Tragacanth gum (TG) as a polysaccharide-based biopolymer has wound healing and antimicrobial properties. In this study, keratin was extracted from protein-based chicken feather by using reduction hydrolysis (sodium sulfide), and nanogels of keratin and TG composites at different ratios were produced by using the chemical cross-linking method. Then, cinnamon (5 and 10%) as an antibacterial herbal extract was added to the nanogels and coated on cotton fabric. The morphology and size of the composite nanogels, chemical structure, biological, and antibacterial properties were evaluated. According to DLS results, TGK2:1 (ratio of TG to keratin = 2:1) had the minimum size (80 nm) and PDI (0.1), and therefore, this sample was chosen as the optimum one. FESEM and TEM images showed the semi-spherical shape of the produced nanogels. FTIR spectra revealed the possible hydrogen bonding between the components, and the formation of disulfide bonds after the addition of hydrogen peroxide was confirmed by XPS. After loading cinnamon into the nanogels, an increase in size was observed from 80 nm for free-nanogel to 85 and 105 nm for 5 and 10% extract-loaded nanogels, respectively. Besides, more cinnamon was released from the treated fabrics by increasing time and cinnamon concentration. The antibacterial test exhibited good antibacterial properties against both Gram-positive and Gram-negative bacteria. Finally, MTT assay approved the biocompatibility of the produced nanogels for potential use in medical textiles.

scholarly journals Antimicrobial Potential of Silver Nanoparticles Synthesized Using Medicinal Herb Coptidis rhizome

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2268 ◽  
Author(s):  
Garima Sharma ◽  
Ju-Suk Nam ◽  
Ashish Sharma ◽  
Sang-Soo Lee
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Inductively Coupled Plasma ◽  
Antimicrobial Property ◽  
Spherical Shape ◽  
Silver Ions ◽  
Average Diameter ◽  
Xrd Analysis ◽  
Visible Absorption ◽  
Biosynthesized Agnps

Coptidis rhizome contains several alkaloids that are bioactive agents of therapeutic value. We propose an eco-friendly method to synthesize biocompatible silver nanoparticles (AgNPs) using the aqueous extract of Coptidis rhizome. Silver ions were reduced to AgNPs using the aqueous extract of Coptidis rhizome, indicating that Coptidis rhizome can be used for the biosynthesis of AgNPs. The time and the concentration required for conversion of silver ions into AgNPs was optimized using UV-absorbance spectroscopy and inductively coupled plasma spectroscopy (ICP). Biosynthesized AgNPs showed a distinct UV-Visible absorption peak at 420 nm. ICP analysis showed that the time required for the completion of biosynthesis was around 20 min. Microscopic images showed that nanoparticles synthesized were of spherical shape and the average diameter of biosynthesized AgNPs was less than 30 nm. XRD analysis also confirmed the size of AgNps and revealed their crystalline nature. The interaction of AgNPs with phytochemicals present in Coptidis rhizome extract was observed in FTIR analysis. The antimicrobial property of AgNPs was evaluated using turbidity measurements. Coptidis rhizome-mediated biosynthesized AgNPs showed significant anti-bacterial activities against Escherichia coli and Staphylococcus aureus that are commonly involved in various types of infections, indicating their potential as an effective anti-bacterial agent.

scholarly journals Study of Antibacterial Properties of Ziziphus mauritiana based Green Synthesized Silver Nanoparticles against Various Bacterial Strains

2020 ◽  
Vol 12 (4) ◽  
pp. 1484 ◽  
Author(s):  
M. Asimuddin ◽  
Mohammed Rafi Shaik ◽  
Neeshat Fathima ◽  
M. Shaistha Afreen ◽  
Syed Farooq Adil ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Low Cost ◽  
Preliminary Investigation ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Ziziphus Mauritiana ◽  
Bacterial Strains ◽  
Vis Spectroscopy ◽  
Ft Ir

Due to their low cost and environmentally friendly nature, plant extracts based methods have gained significant popularity among researchers for the synthesis of metallic nanoparticles. Herein, green synthesis of silver nanoparticles was performed using the aqueous solution of Ziziphus mauritiana leaves extract (ZM-LE) as a bio-reducing agent. The as-obtained silver nanoparticles were characterized by using UV-Vis spectroscopy, XRD (X-ray diffraction), TEM (transmission electron microscopy), and FT-IR (Fourier-transform infrared spectroscopy). In addition, the effects of the concentrations of the leaves extract, silver nitrate, and the temperature on the preparation of nanoparticles were also investigated. In order to determine the nature of secondary metabolites present in leaves extract, a preliminary investigation of phytoconstituents was carried out using different methods including Folin-Ciocalteu and AlCl3 methods. The results have indicated the presence of a considerable amount of phenolic and flavonoid contents in the leaves extract, which are believed to be responsible for the reduction of silver ions and stabilization of resulting nanoparticles. Indeed, the FT-IR spectrum of silver nanoparticles also confirmed the presence of residual phytomolecules of leaves extract as stabilizing ligands on the surface of nanoparticles. The antibacterial properties of as-obtained silver nanoparticles were tested against various bacterial strains including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis. The nanoparticles strongly inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 2.5 μg/ml and moderately inhibited the growth of E. coli with a MIC of 5 μg/ml.

Antibacterial and Hemolytic Activity of Green Silver Nanoparticles from Catharanthus roseus

1970 ◽  
Vol 9 (1) ◽  
pp. 3112-3117 ◽  
Author(s):  
A. Raja ◽  
S. Mohamed Salique ◽  
P. Gajalakshmi ◽  
Arthur James
Keyword(s):  
Silver Nanoparticles ◽  
Cardiac Glycosides ◽  
Room Temperature ◽  
Silver Ions ◽  
Major Constituent ◽  
Colloidal Silver ◽  
Capping Agent ◽  
Phytochemical Study ◽  
One Step

The objective of this research was to standardize the Green synthesis of silver nanoparticles by C.roseus and to evaluate them for their potential against Mycobacteral tuberculosis. Phytochemical study realized the presence flavonoids, saponins, alkaloids, cardiac glycosides, steroids and tannins. Colloidal silver nanoparicle was synthesized by one step green reduction at room temperature and characterized by UV and TEM. An effort made to find out the capping agent revealed that the TLC fraction with 0.7 Rf value showed the reduction of silver ions. GCMS analysis of TLC fraction showed the presence of nine different phytochemical which includes pentadecane and piperdine carboxylic acid as major constituent. The nanoparticle diameter is in the range of 38- 52 nm and stable for 30 days under room temperature. The bactericidal activity of synthesized nanoparticle was  24 ± 0.04 and 22 ± 0.62 mm respectively against M.smegmatis and M.tuberculosis. The in vitro hemolytic assessment of silver nanoparticles was found to be safe at maximum of 1-5 µg/mL and toxic between 15- 50 µg/ml. Further this data encourages that theses biologically synthesized nanoparticles were found to be less toxic and more effective against mycobacterial strains.

scholarly journals Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4479
Author(s):  
Pei-Jun Li ◽  
Jiang-Juan Pan ◽  
Li-Jun Tao ◽  
Xia Li ◽  
Dong-Lin Su ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Antibacterial Activities ◽  
Synthesis Process ◽  
Biological Synthesis ◽  
X Ray Diffraction ◽  
Aspergillus Japonicus ◽  
Transmission Electron ◽  
Naoh Solution

The present study focuses on the biological synthesis, characterization, and antibacterial activities of silver nanoparticles (AgNPs) using extracellular extracts of Aspergillus japonicus PJ01.The optimal conditions of the synthesis process were: 10 mL of extracellular extracts, 1 mL of AgNO3 (0.8 mol/L), 4 mL of NaOH solution (1.5 mol/L), 30 °C, and a reaction time of 1 min. The characterizations of AgNPs were tested by UV-visible spectrophotometry, zeta potential, scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric (TG) analyses. Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag+ was reduced by the extracellular extracts, which consisted chiefly of soluble proteins and reducing sugars. In this work, AgNO3 concentration played an important role in the physicochemical properties and antibacterial properties of AgNPs. Under the AgNO3 concentration of 0.2 and 0.8 mol/L, the diameters of AgNPs were 3.8 ± 1.1 and 9.1 ± 2.9 nm, respectively. In addition, smaller-sized AgNPs showed higher antimicrobial properties, and the minimum inhibitory concentration (MIC) values against both E. coli and S. aureus were 0.32 mg/mL.

Preparation and characterization of silver nanoparticle-porphyrazine composites

2013 ◽  
Vol 17 (10) ◽  
pp. 928-933
Author(s):  
Altuğ Mert Sevim ◽  
Ayşe Selda Keskin ◽  
Ahmet Gül
Keyword(s):  
Silver Nanoparticles ◽  
Chemical Reduction ◽  
Reduction Process ◽  
Silver Ions ◽  
Organic Medium ◽  
Nano Particles ◽  
Effective Manner ◽  
Vis Spectroscopy ◽  
One Step

A one step chemical reduction process was used for the preparation of hydrophilic silver nanoparticles ( AgNP ) using silver nitrate, sodiumborohydride and polyvinylpyrolidone as stabilizer. In the case of hydrophobic silver nanoparticles reduced silver ions were stabilized with cetyl trimethylammonium bromide (CTAB). The resultant nano particles were characterized by absoption spectra and their interactions with cationic cobalt (QCoPz) and neutral magnesium (MgPz) porphyrazines in water and in organic medium were investigated by using UV-vis spectroscopy and zeta potential techniques. It is confirmed that both metalloporphyrazine molecules interact with silver nanoparticles in an effective manner. The possible arrangement of the porphyrazines on the surfaces of the hydrophilic and hydrophobic AgNPs has been also discussed according to obtained spectroscopic results. These well-characterized novel AgNP -metalloporphyrazine composites are expected to be useful in optical and catalytic applications.

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