scholarly journals Concentration and Purification of CollagenProteins by Ultrafiltration

2020 ◽  
Vol 70 (12) ◽  
pp. 4611-4616
Keyword(s):  
Electron Microscopy ◽  
Protein Purification ◽  
Physicochemical Characteristics ◽  
Regenerated Cellulose ◽  
Cellulose Membrane ◽  
Collagen Concentration ◽  
Collagen Solution ◽  
Flow Filtration ◽  
Cellulose Membranes ◽  
Ultrafiltration Process

The objective of the present study was to evaluate the performance of the ultrafiltration process on the recovery of collagen solution constituents at laboratory scale, using a tangential flow filtration, with flat regenerated cellulose membrane (5000 Da). Also permeate flows were evaluated along with the physicochemical characteristics of the concentrates, the permeates and feed solutions. The regenerated cellulose membranes were morphological studied by electron microscopy and characterized by water solutions permeation at different pressures. Keywords: collagen, concentration, protein, purification, ultrafiltration

Hemodialysis Membrane Prepared from Bacterial Cellulose/Lithium Chloride/N,N-Dimethylacetamide Solution

2014 ◽  
Vol 1048 ◽  
pp. 395-399 ◽  
Author(s):  
Jia Zhi Yang ◽  
Guang Mei Liu ◽  
Dong Ping Sun
Keyword(s):  
Tensile Strength ◽  
Bacterial Cellulose ◽  
Phase Inversion ◽  
Inversion Method ◽  
Regenerated Cellulose ◽  
Vitamin B ◽  
Cellulose Membrane ◽  
Cellulose Membranes ◽  
Phase Inversion Method ◽  
Percentage Porosity

A new bacterial cellulose regenerated cellulose membrane (RBC) has been prepared by phase inversion method in LiCl/DMAC solution. The effects of coagulation concentrations on the physical properties of the RBC membranes were studied and optimized. Features of the RBC of membranes obtained using 0%~40% N,N-Dimethylacetamide (DMAC), such as water absorption percentage, porosity (Pr), ultrafilter rate (UFR), and tensile strength were investigated. The RBC membranes prepared with 40% DMAC solution as coagulant exhibit a smooth surface and a high tensile strength with suitable UFR. RBC showed improved permeabilities for urea and vitamin B12when compared with commercial cellulose membranes.

scholarly journals Cellulose Membranes in the Treatment of Spent Deep Eutectic Solvent Used in the Recovery of Lignin from Lignocellulosic Biomass

Membranes ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 86
Author(s):  
Vadim Ippolitov ◽  
Ikenna Anugwom ◽  
Robin van Deun ◽  
Mika Mänttäri ◽  
Mari Kallioinen-Mänttäri
Keyword(s):  
Lactic Acid ◽  
Lignocellulosic Biomass ◽  
Pure Water ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Regenerated Cellulose ◽  
Polymeric Compounds ◽  
Uf Membranes ◽  
Cellulose Membranes ◽  
Ultrafiltration Process

Ultrafiltration was employed in the purification of spent Deep Eutectic Solvent (DES, a mixture of choline chloride and lactic acid, 1:10, respectively) used in the extraction of lignin from lignocellulosic biomass. The aim of this was to recover different lignin fractions and to purify spent solvent. The results revealed that the commercial regenerated cellulose membranes—RC70PP and Ultracel 5 kDa UF membranes—could be used in the treatment of the spent DES. The addition of cosolvent (ethanol) to the spent DES decreased solvent’s viscosity, which enabled filtration. With two-pass ultrafiltration process with 10 kDa and 5 kDa membranes about 95% of the dissolved polymeric compounds (lignin and hemicelluloses) were removed from the spent DES. The utilized membranes also showed the capability to fractionate polymeric compounds into two fractions—above and under 10,000 Da. Moreover, the 10 kDa cellulose-based membrane showed good stability during a continuous period of three weeks exposure to the solution of DES and ethanol. Its pure water permeability decreased only by 3%. The results presented here demonstrate the possibility to utilize cellulose membranes in the treatment of spent DES to purify the solvent and recover the interesting compounds.

Multilevel/hierarchical nanocomposite imprinted regenerated cellulose membranes for high-efficiency separation: A selective recognition method with Au/PDA-loaded surface

2021 ◽  
Author(s):  
Ming Yan ◽  
Yilin Wu ◽  
Rongxin Lin ◽  
Faguang Ma ◽  
Zhongyi Jiang
Keyword(s):  
Molecular Imprinting ◽  
High Efficiency ◽  
Selective Separation ◽  
Selective Recognition ◽  
Regenerated Cellulose ◽  
Separation Performance ◽  
Recognition Method ◽  
Membrane Materials ◽  
Loaded Surface ◽  
Cellulose Membranes

Although many researchers have done lots of studies on improving the selective separation performance of membrane materials, conceptions and applications of membrane-based molecular imprinting separation&recognition with both high permselectivity and...

A graphene oxide facilitated a highly porous and effective antibacterial regenerated cellulose membrane containing stabilized silver nanoparticles

Cellulose ◽  
2014 ◽  
Vol 21 (6) ◽  
pp. 4261-4270 ◽  
Author(s):  
Soon Wei Chook ◽  
Chin Hua Chia ◽  
Sarani Zakaria ◽  
Mohd Khan Ayob ◽  
Nay Ming Huang ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Graphene Oxide ◽  
Regenerated Cellulose ◽  
Cellulose Membrane ◽  
Highly Porous

Isolation of Immunoglobulin from Chicken Egg Yolk using Single-Stage Ultrafiltration with 100-kDa Regenerated Cellulose Membranes

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Jianguo Liu ◽  
Juan Yang ◽  
Hai Xu ◽  
Hu Zhu ◽  
Jianbo Qu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Egg Yolk ◽  
Single Stage ◽  
Regenerated Cellulose ◽  
Cellulose Membrane ◽  
Permeate Flux ◽  
Operational Parameters ◽  
Solution Ph ◽  
Chicken Egg ◽  
Chicken Egg Yolk

The aim of this work is to develop a membrane-based cost-effective process for the rapid isolation of immunoglobulin from chicken egg yolk. It was found that a single-stage ultrafiltration using a 100 kDa molecular weight cut-off regenerated cellulose membrane could be employed to isolate immunoglobulin from the crude feedstock. The effects of operational parameters (solution pH, ionic strength, stirring speed and permeate flux) on the transmission of immunoglobulin and the presence of impurity protein with molecular weight close to immunoglobulin were quantified using the parameter scanning ultrafiltration technique. Under optimized conditions, the purity of immunoglobulin obtained was about 85 percent after the single-stage ultrafiltration process, and the recovery of immunoglobulin from the feedstock was 91 percent.

X-ray induced degradation of regenerated cellulose membrane films

1995 ◽  
Vol 50 (1) ◽  
pp. 125-130 ◽  
Author(s):  
Yoshio Kawano ◽  
Amadeu J.M. Logarezzi
Keyword(s):  
Regenerated Cellulose ◽  
Cellulose Membrane ◽  
X Ray

Surface Modification of Cellulose Membrane by Air Plasma Treatment

2013 ◽  
Vol 770 ◽  
pp. 112-115
Author(s):  
Nawal Binhayeeniyi ◽  
Adinan Jehsu ◽  
Mancharee Sukpet ◽  
Safitree Nawae
Keyword(s):  
Surface Modification ◽  
Plasma Treatment ◽  
Surface Modifications ◽  
Contact Angles ◽  
Cellulose Membrane ◽  
Air Plasma ◽  
Before And After ◽  
Cellulose Membranes ◽  
Hydrophilic Membranes ◽  
Air Plasma Treatment

Low-temperature air plasma was used to treat the cellulose membranes by varying the period of time from 10 to 30 minutes. The surfaces of membranes were changed from hydrophobic to hydrophilic membranes. The contact angles of treated membranes were increased when increasing time to treat. The surface modifications of membrane before and after treated were characterized by SEM. It is shown that air plasma treatment is used to improve the roughness. The dielectric property was also studied.

Measurement of the Particle Size of a High-Lightfastness Direct Dye in Cellulose by p-Nitrophenol Adsorption

1977 ◽  
Vol 47 (5) ◽  
pp. 347-350 ◽  
Author(s):  
Charles H. Giles ◽  
Ronald Haslam
Keyword(s):  
Electron Microscopy ◽  
Particle Size ◽  
Aggregation Number ◽  
Regenerated Cellulose ◽  
Cellulose Film ◽  
Adsorption Method ◽  
Regenerated Cellulose Film ◽  
Direct Dye ◽  
High Concentrations ◽  
Film Weight

The p-nitrophenol adsorption method has been used to determine the specific surface, and hence the particle size and aggregation number, of a high-lightfastness direct dye (C.I. Direct Green 26) in regenerated cellulose film. The particle size found at high concentrations is of the same order as that found for other dyes of this type, in cellulose film, by Weissbein and Coven, using electron microscopy. The aggregation number rises rapidly with rise in dye concentration in the film to a maximum of the order of 106 at shade depths above about two percent pure dye on film weight.

scholarly journals Affinity Membranes and Monoliths for Protein Purification

Membranes ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 1 ◽  
Author(s):  
Eleonora Lalli ◽  
Jouciane S. Silva ◽  
Cristiana Boi ◽  
Giulio C. Sarti
Keyword(s):  
Binding Capacity ◽  
Stationary Phases ◽  
Packed Column ◽  
Downstream Processing ◽  
Regenerated Cellulose ◽  
Affinity Capture ◽  
Scale Characterization ◽  
Set Up ◽  
Cellulose Membranes ◽  
Chromatographic Media

Affinity capture represents an important step in downstream processing of proteins and it is conventionally performed through a chromatographic process. The performance of this step highly depends on the type of matrix employed. In particular, resin beads and convective materials, such as membranes and monoliths, are the commonly available supports. The present work deals with non-competitive binding of bovine serum albumin (BSA) on different chromatographic media functionalized with Cibacron Blue F3GA (CB). The aim is to set up the development of the purification process starting from the lab-scale characterization of a commercially available CB resin, regenerated cellulose membranes and polymeric monoliths, functionalized with CB to identify the best option. The performance of the three different chromatographic media is evaluated in terms of BSA binding capacity and productivity. The experimental investigation shows promising results for regenerated cellulose membranes and monoliths, whose performance are comparable with those of the packed column tested. It was demonstrated that the capacity of convective stationary phases does not depend on flow rate, in the range investigated, and that the productivity that can be achieved with membranes is 10 to 20 times higher depending on the initial BSA concentration value, and with monoliths it is approximately twice that of beads, at the same superficial velocity.

scholarly journals Influence of heat treatment on physicochemical and rheological characteristics of natural yogurts

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2489 ◽  
Author(s):  
Juliana Aparecida Célia ◽  
Marco Antônio Pereira da Silva ◽  
Kênia Borges de Oliveira ◽  
Diene Gonçalves Souza ◽  
Ligia Campos de Moura Silva ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Heat Treatment ◽  
Titratable Acidity ◽  
Physicochemical Characteristics ◽  
Rheological Characteristics ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Lactic Bacteria ◽  
Milk Treatment ◽  
Scanning Electron

The aim of this study was to assess the influence of heat treatment on physicochemical and rheological characteristics of natural yogurts, as well as the influence of lyophilization process on natural yogurts after reconstitution. In the first experiment, three yogurt treatments were processed, as follows: Treatment 1, yogurt produced with raw refrigerated milk; Treatment 2, yogurt produced with refrigerated pasteurized milk; and Treatment 3, yogurt produced with UHT (ultra-high temperature) milk, in addition to analyses of fat, protein, moisture, titratable acidity, and pH. The shelf life of yogurts at 1, 8, 15, 22, and 29 days of storage, as well as pH, acidity, syneresis, viscosity, viable lactic bacteria, and total coliforms were also assessed. In the second experiment, yogurts were submitted to lyophilization process, performed by scanning electron microscopy analysis and subsequently in those reconstituted, in addition to being assessed the physicochemical, rheological, and viable lactic bacteria characteristics. The results found in the first experiment showed that heat treatment was positive for viscosity, syneresis, and lactic bacteria, being viable until the 15th day of storage only for yogurts submitted to heat treatment. In the second experiment, lyophilization preserved the physicochemical characteristics of yogurts, but the number of initial lactic bacteria was different, also negatively affecting yogurt viscosity.

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