Preparation of Transglutaminase Cross-Linked Tilapia Scale Collagen Microspheres Containing L-Ascorbic Acid

2012 ◽  
Vol 535-537 ◽  
pp. 2307-2311
Author(s):  
Peng Li ◽  
Xuan Ri Shen ◽  
Zhou Wei Duan ◽  
Zhang Han Wang
Keyword(s):  
Ascorbic Acid ◽  
Ultrasonic Wave ◽  
Bioactive Compounds ◽  
Encapsulation Efficiency ◽  
Incubation Temperature ◽  
Orthogonal Experiment ◽  
Coating Material ◽  
Cross Linking ◽  
Biocompatible Material ◽  
Tilapia Scale

With the goal to develop biocompatible material collagen extracted from tilapia scale as coating material to encapsulate bioactive compounds, a microsphere containing L-ascorbic acid as a model is developed, using natural nontoxic transglutaminase (TG) as cross-linking agent. Ultrasonic wave was applied in the process to assist preparing microcapsule. Factors of emulsifier, TG, ratio of L-ascorbic acid and collagen, incubation temperature were discussed. The formulation from orthogonal experiment was of the optimal encapsulation efficiency 81.17%.It is found that the collagen extracted from tilapia scale is a potential biomaterial.

scholarly journals Formation of Amphiphilic Molecules from the Most Common Marine Polysaccharides, toward a Sustainable Alternative?

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4445
Author(s):  
Tiphaine Wong ◽  
Lorette Brault ◽  
Eric Gasparotto ◽  
Romuald Vallée ◽  
Pierre-Yves Morvan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Carboxylic Acid ◽  
Bioactive Compounds ◽  
Biological Properties ◽  
Cross Linking ◽  
Structural Modifications ◽  
Chemical Structures ◽  
Amphiphilic Molecules

Marine polysaccharides are part of the huge seaweeds resources and present many applications for several industries. In order to widen their potential as additives or bioactive compounds, some structural modifications have been studied. Among them, simple hydrophobization reactions have been developed in order to yield to grafted polysaccharides bearing acyl-, aryl-, alkyl-, and alkenyl-groups or fatty acid chains. The resulting polymers are able to present modified physicochemical and/or biological properties of interest in the current pharmaceutical, cosmetics, or food fields. This review covers the chemical structures of the main marine polysaccharides, and then focuses on their structural modifications, and especially on hydrophobization reactions mainly esterification, acylation, alkylation, amidation, or even cross-linking reaction on native hydroxyl-, amine, or carboxylic acid functions. Finally, the question of the necessary requirement for more sustainable processes around these structural modulations of marine polysaccharides is addressed, considering the development of greener technologies applied to traditional polysaccharides.

scholarly journals BIOACTIVE COMPOUNDS DURING THE MATURATION OF FOUR FRUITS NATIVE TO THE RESTINGA FOREST OF CEARA

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
NIGÉRIA PEREIRA GONÇALVES ◽  
ELISEU MARLÔNIO PEREIRA DE LUCENA ◽  
ORIEL HERRERA BONILLA ◽  
MÁRCIA RÉGIA SOUZA DA SILVEIRA ◽  
FRANCISCA JÉSSICA COSTA TAVARES
Keyword(s):  
Ascorbic Acid ◽  
Bioactive Compounds ◽  
Botanical Garden ◽  
Total Chlorophyll ◽  
Total Carotenoids ◽  
Plant Ecophysiology ◽  
Randomized Design ◽  
Total Anthocyanins ◽  
Maturation Stages ◽  
Completely Randomized Design

ABSTRACT Fruits provide not only essential nutrients for food, but also bioactive compounds that promote health benefits and help reducing the risk of developing non-communicable chronic diseases. In this sense, this work aimed at quantifying bioactive compounds during the maturation of four fruits native to the Restinga forest of Ceara. Myrtle fruits (Eugenia punicifolia (Kunth) DC.) were collected at the Botanical State Park of Ceara, Caucaia-CE, and guajiru (Chrisobalanus icaco L.), manipuça (Mouriri cearensis Huber) and murici-pitanga fruits (Byrsonima gardneriana A. Juss.) at the Botanical Garden of São Gonçalo, São Gonçalo do Amarante-CE. Fruits were collected at different stages (E) and transported to the Laboratory of Plant Ecophysiology, being characterized into five or six maturation stages according to the bark color, then processed and frozen for the following physicochemical and chemical evaluations: ascorbic acid, total chlorophyll, total carotenoids, total anthocyanins and yellow flavonoids. A completely randomized design was used, with five or six treatments, depending on the maturation stages of fruits and four replicates. Murici-pitanga had higher contents of ascorbic acid (ascorbic acid 646.23 mg/100 g E5), total carotenoids (6.13 mg/100 g E5) and total anthocyanins (7.99 mg/100 g E2); and myrtle had higher contents of total chlorophyll (11.05 mg/100 g E1) and yellow flavonoid (69.11 mg/100 g E2). There are positive and significant correlations between chlorophyll and carotenoid (R= 0.99; P <0.01) for manipuça and between anthocyanin and yellow flavonoid (R= 0.97; P <0.05) for murici-pitanga fruits; however, the correlation is negative and significant between ascorbic acid and yellow flavonoids (R= -0.98; P <0.05) for myrtle fruits. It could be concluded that murici-pitanga and myrtle fruits had the highest contents of bioactive compounds with the highest levels, therefore both fruits can be recommended to be commercially exploited by the food, cosmetic and phytomedicine sectors.

scholarly journals Synthesis and Optimization of Chitosan Nanoparticles Loaded with L-Ascorbic Acid and Thymoquinone

Nanomaterials ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 920 ◽  
Author(s):  
Nurhanisah Othman ◽  
Mas Masarudin ◽  
Cha Kuen ◽  
Nurul Dasuan ◽  
Luqman Abdullah ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Ascorbic Acid ◽  
Infrared Spectroscopy ◽  
Encapsulation Efficiency ◽  
High Surface ◽  
Chitosan Nanoparticles ◽  
Polydispersity Index ◽  
Ionic Gelation ◽  
Surface Areas ◽  
Efficient Delivery

The combination of compounds with different classes (hydrophobic and hydrophilic characters) in single chitosan carrier is a challenge due to the hydrophilicity of chitosan. Utilization of l-ascorbic acid (LAA) and thymoquinone (TQ) compounds as effective antioxidants is marred by poor bioavailability and uptake. Nanoparticles (NPs) solved the problem by functioning as a carrier for them because they have high surface areas for more efficient delivery and uptake by cells. This research, therefore, synthesized chitosan NPs (CNPs) containing LAA and TQ, CNP-LAA-TQ via ionic gelation routes as the preparation is non-toxic. They were characterized using electron microscopy, zetasizer, UV–VIS spectrophotometry, and infrared spectroscopy. The optimum CNP-LAA-TQ size produced was 141.5 ± 7.8 nm, with a polydispersity index (PDI) of 0.207 ± 0.013. The encapsulation efficiency of CNP-LAA-TQ was 22.8 ± 3.2% for LAA and 35.6 ± 3.6% for TQ. Combined hydrophilic LAA and hydrophobic TQ proved that a myriad of highly efficacious compounds with poor systemic uptake could be encapsulated together in NP systems to increase their pharmaceutical efficiency, indirectly contributing to the advancement of medical and pharmaceutical sectors.

scholarly journals Bioactive Compounds, Vitamins and Minerals Composition of Freeze-dried Grewia asiatica L. (Phalsa) Pulp and Seed Powder

2019 ◽  
Vol 38 (03) ◽  
Author(s):  
Kiran Bala ◽  
Aradhita Barmanray
Keyword(s):  
Heavy Metals ◽  
Ascorbic Acid ◽  
Vitamin A ◽  
Bioactive Compounds ◽  
Functional Foods ◽  
Total Phenols ◽  
Total Anthocyanin ◽  
Freeze Dried ◽  
Total Anthocyanins ◽  
Β Carotene

Present study was directed to analyze and compare the bioactive compounds (total phenols, total anthocyanins), vitamins (ascorbic acid, β-carotene, vitamin A), minerals including Ca, Mg, Na, P, K, Fe, Cu, Zn, Co, Mn and heavy metals (Cd, Hg, Pb) of freeze-dried (lyophilized) phalsa pulp and seed powder. In lyophilized pulp powder (LPP) higher amount of total phenols (78.11 mg/100g), total anthocyanin (82.94 mg/100g), ascorbic acid (5.21 mg/100g), β-carotene (0.54 µg/100g), vitamin A (0.89 I.U.) were observed than lyophilized seed powder (LSP). Na, K, Mg and Co (0.41, 0.39, 1.08, 0.46 mg/100g, respectively) were higher in LPP as compared to LSP (0.29, 0.11, 0.76 and 0.40 mg/100g, respectively) whereas, Ca, P and Cu were detected more in LSP. This study opens the prospect of using dry phalsa powder in the preparation of various nutraceutical and functional foods for their therapeutic as well as prophylactic purposes.

scholarly journals Enhancing or Inhibitory Effect of Fruit or Vegetable Bioactive Compound on Aspergillus niger and A. oryzae

2021 ◽  
Vol 8 (1) ◽  
pp. 12
Author(s):  
Gülru Bulkan ◽  
Sitaresmi Sitaresmi ◽  
Gerarda Tania Yudhanti ◽  
Ria Millati ◽  
Rachma Wikandari ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Aspergillus Niger ◽  
Bioactive Compounds ◽  
Ellagic Acid ◽  
Microbial Growth ◽  
Biomass Yield ◽  
Antimicrobial Effect ◽  
Bioactive Compound ◽  
Inhibitory Effect ◽  
Processing Wastes

Fruit and vegetable processing wastes are global challenges but also suitable sources with a variety of nutrients for different fermentative products using bacteria, yeast or fungi. The interaction of microorganisms with bioactive compounds in fruit waste can have inhibitory or enhancing effect on microbial growth. In this study, the antimicrobial effect of 10 bioactive compounds, including octanol, ellagic acid, (−)-epicatechin, quercetin, betanin, ascorbic acid, limonene, hexanal, car-3-ene, and myrcene in the range of 0–240 mg/L on filamentous fungi Aspergillus oryzae and Aspergillus niger were investigated. These fungi were both found to be resistant to all compounds except octanol, which can be used as a natural antifungal agent, specifically against A. oryzae and A. niger contamination. On the contrary, polyphenols (quercetin and ellagic acid), ascorbic acid, and hexanal enhanced A. niger biomass yield 28%, 7.8%, 16%, and 6%, respectively. Furthermore, 240 mg/L car-3-ene was found to increase A. oryzae biomass yield 8%, while a 9% decrease was observed at lower concentration, 24 mg/L. Similarly, up to 17% decrease of biomass yield was observed from betanin and myrcene. The resistant nature of the fungi against FPW bioactive compounds shows the potential of these fungi for further application in waste valorization.

scholarly journals Polyphenol-Based Microencapsulated Extracts as Novel Green Insecticides for Sustainable Management of Polyphagous Brown Marmorated Stink Bug (Halyomorpha halys Stål, 1855)

2020 ◽  
Vol 12 (23) ◽  
pp. 10079
Author(s):  
Ivana Pajač Živković ◽  
Slaven Jurić ◽  
Marko Vinceković ◽  
Marija Andrijana Galešić ◽  
Marijan Marijan ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Encapsulation Efficiency ◽  
The United States ◽  
Fickian Diffusion ◽  
Growth Stages ◽  
Halyomorpha Halys ◽  
Potential Candidate ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Fruit Growing

The brown marmorated stink bug (Halyomorpha halys Stål, 1855) is an invasive polyphagous species that threatens fruit growing both in the United States and Europe. Many pesticide active ingredients have been studied in H. halys management, but for sustainable fruit growing, which implies the reduction of chemical harm to the environment, new safe insecticides should be implemented into the practice. For this purpose, novel green insecticide based on natural polyphenols of species Stevia rebaudiana (Bertoni) Bertoni and Aronia melanocarpa (Michx.) Elliott 1821 was developed. Stevia leaves (SLE) and Aronia pomace (APE) aqueous extracts were prepared using the ultrasound-assisted extraction method. Optimal extraction conditions for bioactive compounds (total polyphenols, flavonoids, anthocyanins, and flavan-3-ols, respectively) and antioxidant activity were determined using response surface methodology. Bioactive compounds rich SLE and APE were encapsulated in calcium alginate microparticles by the ionic gelation method. Physicochemical characteristics (morphology, size, encapsulation efficiency, loading capacity, and swelling) of microparticles showed very good properties with especially high encapsulation efficiency. Fitting to simple Korsmeyer–Peppa’s empirical model revealed that the underlying release mechanism of polyphenols is Fickian diffusion. SLE loaded microparticles showed very good pesticidal efficiency against Halyomorpha halys, especially on younger larval stages after both contact and digestive treatment. Microparticles loaded with APE did not achieve satisfactory digestive efficiency, but a certain toxic impact has been observed at contact application on all H. halys growth stages. Microparticles loaded with SLS exhibited prolonged insecticidal action against H. halys and could be a potential candidate as a green insecticide whose application could increase fruit growing safety.

scholarly journals Effect of Processing Methods and Storage Time on the Content of Bioactive Compounds in Blue Honeysuckle Berry Purees

Agronomy ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 860 ◽  
Author(s):  
Anna Grobelna ◽  
Stanisław Kalisz ◽  
Marek Kieliszek
Keyword(s):  
Ascorbic Acid ◽  
Thermal Treatment ◽  
Bioactive Compounds ◽  
Storage Time ◽  
Lower Content ◽  
Total Polyphenols ◽  
Lonicera Caerulea ◽  
Processing Methods ◽  
And Storage ◽  
Blue Honeysuckle

In this study, we aimed to investigate the effect of processing methods and storage time on selected parameters of quality in the purees of blue honeysuckle berries. We investigated the content of bioactive compounds such as anthocyanins, L-ascorbic acid, and total polyphenols (TPs). We tested four processing methods and two varieties of blue honeysuckle berry (Lonicera caerulea L.var. Sineglaska and Volshebnica). The purees were analyzed immediately after production, and after two and four months of storage at 20 °C without exposure to light. According to our results, thermal treatment of the fruits for 5 min resulted in obtaining purees with a higher content of anthocyanins and TPs, but lower content of L-ascorbic acid. However, sieving fruits that were not thermally treated resulted in a higher content of L-ascorbic acid. Furthermore, purees prepared from the fruits of the Volshebnica cultivar were richer in bioactive compounds than that of purees prepared from the Sineglaska cultivar. In addition, the content of anthocyanins, L-ascorbic acid, and TPs decreased with storage time.

Two-Photon Sensing of Dopamine by Using Au Nanoparticles

2015 ◽  
Vol 738-739 ◽  
pp. 31-37
Author(s):  
Cui Feng Jiang ◽  
Neng Yue Gao ◽  
Qi Sheng Wu ◽  
Yu Sun ◽  
Qing Hua Xu
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Detection Limit ◽  
Metal Ions ◽  
Gold Nanoparticle ◽  
Au Nanoparticles ◽  
Cross Linking ◽  
Au Nps ◽  
Two Photon ◽  
Catechol Group

In this paper, we demonstrated a gold nanoparticle based two-photon photoluminescence (TPPL) assay for the detection of dopamine with the detection limit of 0.3 μM. Protonated dopamine molecules can bind bidentately to surface of gold atoms through the catechol group. The adsorption of dopamine displaces the stabilizing agent of citrate groups, and neutralizes the charge of solution, resulting in non-cross linking aggregation of Au NPs. Aggregation of Au NPs could induce significantly enhanced TPPL. Thus, a simple TPPL assay was designed. We have shown that when Au NPs solution was mixed with dopamine, TPPL intensity increased by about 47 times. The mechanism of sensing assay has been discussed. In addition, the TPPL assay was highly selective to dopamine and it can distinguish from uric acid, ascorbic acid and metal ions.

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