Application of Microbial Polyesters-Polyhydroxyalkanoates as Tissue Engineering Materials

2005 ◽  
Vol 288-289 ◽  
pp. 437-440 ◽  
Author(s):  
Guo Qiang Chen ◽  
Qiong Wu ◽  
Ya Wu Wang ◽  
Zhong Zheng
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Surface Treatment ◽  
Surface Properties ◽  
Three Dimensional ◽  
Material Surface ◽  
New Class ◽  
Molecular Studies ◽  
Cartilage Cells ◽  
Engineering Materials

Poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) has improved mechanical properties over the existing PHA and our results have shown that PHBHHx has better biocompatibility over polyhydroxybutyrate (PHB) and polylactic acid (PLA). Surface treatment with lipases dramatically changed the material surface properties and increased the biocompatibility of the PHBHHx. PHBHHx and its PHB blends had been used to make three dimensional structures and it has been found that cartilage, osteoblast, and fibroblasts all showed strong growth on the PHBHHx scaffolds. The growth was much better compared with PLA. The molecular studies also showed that mRNA encoding cartilages were strongly expressed when cartilage cells were grown on the PHBHHx. As PHBHHx has strong mechanical properties, easily processible and biodegradable, this material can be used to develop a new class of tissue engineering materials.

scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

scholarly journals Titanium oral implants: surface characteristics, interface biology and clinical outcome

2010 ◽  
Vol 7 (suppl_5) ◽  
Author(s):  
Anders Palmquist ◽  
Omar M. Omar ◽  
Marco Esposito ◽  
Jukka Lausmaa ◽  
Peter Thomsen
Keyword(s):  
Surface Properties ◽  
Cellular Response ◽  
Randomized Clinical Trials ◽  
Three Dimensional ◽  
Cell Communication ◽  
Surface Characteristics ◽  
Titanium Implants ◽  
Material Surface ◽  
Oral Implants

Bone-anchored titanium implants have revolutionized oral healthcare. Surface properties of oral titanium implants play decisive roles for molecular interactions, cellular response and bone regeneration. Nevertheless, the role of specific surface properties, such as chemical and phase composition and nanoscale features, for the biological in vivo performance remains to be established. Partly, this is due to limited transfer of state-of-the-art preparation techniques to complex three-dimensional geometries, analytical tools and access to minute, intact interfacial layers. As judged by the available results of a few randomized clinical trials, there is no evidence that any particular type of oral implant has superior long-term success. Important insights into the recruitment of mesenchymal stem cells, cell–cell communication at the interface and high-resolution imaging of the interface between the surface oxide and the biological host are prerequisites for the understanding of the mechanisms of osseointegration. Strategies for development of the next generation of material surface modifications for compromised tissue are likely to include time and functionally programmed properties, pharmacological modulation and incorporation of cellular components.

scholarly journals Biomimicry in Bio-Manufacturing: Developments in Melt Electrospinning Writing Technology Towards Hybrid Biomanufacturing

2019 ◽  
Vol 9 (17) ◽  
pp. 3540 ◽  
Author(s):  
Ferdows Afghah ◽  
Caner Dikyol ◽  
Mine Altunbek ◽  
Bahattin Koc
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Future Perspective ◽  
Melt Electrospinning ◽  
Wide Range ◽  
Challenges And Opportunities ◽  
Potential Applications ◽  
Homogeneous Cell

Melt electrospinning writing has been emerged as a promising technique in the field of tissue engineering, with the capability of fabricating controllable and highly ordered complex three-dimensional geometries from a wide range of polymers. This three-dimensional (3D) printing method can be used to fabricate scaffolds biomimicking extracellular matrix of replaced tissue with the required mechanical properties. However, controlled and homogeneous cell attachment on melt electrospun fibers is a challenge. The combination of melt electrospinning writing with other tissue engineering approaches, called hybrid biomanufacturing, has introduced new perspectives and increased its potential applications in tissue engineering. In this review, principles and key parameters, challenges, and opportunities of melt electrospinning writing, and particularly, recent approaches and materials in this field are introduced. Subsequently, hybrid biomanufacturing strategies are presented for improved biological and mechanical properties of the manufactured porous structures. An overview of the possible hybrid setups and applications, future perspective of hybrid processes, guidelines, and opportunities in different areas of tissue/organ engineering are also highlighted.

Combined Effects of Scaffold Material and Mechanical Stimulation on the Formation of Tissue Engineered Constructs Using Tendon and Ligament Progenitor Cells

2013 ◽  
Author(s):  
Andrew P. Breidenbach ◽  
Nathaniel A. Dyment ◽  
Yinhui Lu ◽  
Jason T. Shearn ◽  
David W. Rowe ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Progenitor Cells ◽  
Three Dimensional ◽  
Musculoskeletal Injuries ◽  
Combined Effects ◽  
Joint Movement ◽  
Scaffold Material ◽  
Ligament Injuries ◽  
Promising Alternative

Tendon and ligament injuries account for one-third of all musculoskeletal injuries [1]. Collagen fibrils in these mechanosensitive tissues transmit forces to mobilize and stabilize joint movement. Donor tissues used to repair these tissues often lack the mechanical properties of the tissue they are replacing. One promising alternative using tissue engineering combines stem/progenitor cells in three-dimensional tissue engineered constructs (TECs).

scholarly journals Alginate/Gelatin Hydrogels Reinforced with TiO2 and β-TCP Fabricated by Microextrusion-based Printing for Tissue Regeneration

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 457 ◽  
Author(s):  
Rodrigo Urruela-Barrios ◽  
Erick Ramírez-Cedillo ◽  
A. Díaz de León ◽  
Alejandro Alvarez ◽  
Wendy Ortega-Lara
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Average Pore Size ◽  
Three Dimensional ◽  
Engineering Applications ◽  
Average Pore ◽  
Viscous Deformation ◽  
Freeze Dried ◽  
3D Printed

Three-dimensional (3D) printing technologies have become an attractive manufacturing process to fabricate scaffolds in tissue engineering. Recent research has focused on the fabrication of alginate complex shaped structures that closely mimic biological organs or tissues. Alginates can be effectively manufactured into porous three-dimensional networks for tissue engineering applications. However, the structure, mechanical properties, and shape fidelity of 3D-printed alginate hydrogels used for preparing tissue-engineered scaffolds is difficult to control. In this work, the use of alginate/gelatin hydrogels reinforced with TiO2 and β-tricalcium phosphate was studied to tailor the mechanical properties of 3D-printed hydrogels. The hydrogels reinforced with TiO2 and β-TCP showed enhanced mechanical properties up to 20 MPa of elastic modulus. Furthermore, the pores of the crosslinked printed structures were measured with an average pore size of 200 μm. Additionally, it was found that as more layers of the design were printed, there was an increase of the line width of the bottom layers due to its viscous deformation. Shrinkage of the design when the hydrogel is crosslinked and freeze dried was also measured and found to be up to 27% from the printed design. Overall, the proposed approach enabled fabrication of 3D-printed alginate scaffolds with adequate physical properties for tissue engineering applications.

Fabrication of a chitosan/bioglass three-dimensional porous scaffold for bone tissue engineering applications

2014 ◽  
Vol 2 (38) ◽  
pp. 6611-6618 ◽  
Author(s):  
Jun Yang ◽  
Teng Long ◽  
Nan-Fei He ◽  
Ya-Ping Guo ◽  
Zhen-An Zhu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
Three Dimensional ◽  
Bone Defects ◽  
Engineering Applications

A chitosan/bioglass three-dimensional porous scaffold with excellent biocompatibility and mechanical properties has been developed for the treatment of bone defects.

Informatics Challenges in Tissue Engineering and Biomaterials

2003 ◽  
Vol 17 (1) ◽  
pp. 49-54 ◽  
Author(s):  
D. Rekow
Keyword(s):  
Tissue Engineering ◽  
Clinical Performance ◽  
Three Dimensional ◽  
Thin Layers ◽  
Material Surface ◽  
Dental Restorations ◽  
Scaffold Materials ◽  
Ceramic Crowns ◽  
Three Dimensional Configuration

Both tissue engineering and biomaterials have made tremendous strides recently, yet major questions remain unanswered. Tissue-engineered products have come to the market; others are in development. A fundamental issue that informatics could address for tissue engineering is to describe and to predict the cascade of biochemical and cellular reactions that occur as a function of time and implant material: surface texture, microporosity; pore size, density, and connectivity; and three-dimensional configuration. Behavior of ceramics, a subset of tissue-engineering scaffold materials and a mainstay of dental restorations, has been studied extensively for very thin layers and for thicknesses greater than 2 mm. Until recently, little has been known about dentally relevant thickness of 1–2 mm. Results have been surprising and are continuing to develop. Still, at least one fundamental question remains that could be addressed by informatics techniques: Where, along the spectrum of flat-polished material to 10-year clinical in vivo study, can we test to predict clinical performance of all-ceramic crowns accurately?

scholarly journals Textile Finishing Using Polymer Nanocomposites for Radiation Shielding, Flame Retardancy and Mechanical Strength

2021 ◽  
Author(s):  
Sorna Gowri ◽  
◽  
Mohammad Akram Khan ◽  
Avanish Kumar Srivastava ◽  
◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Mechanical Strength ◽  
Surface Treatment ◽  
Surface Properties ◽  
Polymer Nanocomposites ◽  
Flame Retardancy ◽  
Radiation Shielding ◽  
Textile Finishing ◽  
Increasing Demand ◽  
Technical Effort

The uses of nanotechnologies in textiles are strategic and allow textiles to become multifunctional. There is an ever-increasing demand for new functionalities, like flame retardancy, radiation shielding, improved mechanical strength etc., for highly specific applications. There is no industrial supply for the above-mentioned functionalities. Keeping in view of this background, surface treatment becomes one of the most important methods to create new textile properties. Polymer nanocomposites based on coatings for textiles have a huge potential for innovative modifications of surface properties like flame retardancy, radiation shielding and improved mechanical properties, which can be applied with a comparatively low technical effort and at moderate temperatures. This review compiles recent research on polymer nanocomposites for functional finishing of textiles to understand the theoretical and experimental tools on polymer nanocomposites and their applications in textiles.

Improving Surface Properties of Linear Polyethylene by Radiation Measured by Ultra-Nano Indentation Test

2019 ◽  
Vol 952 ◽  
pp. 172-179
Author(s):  
Martin Ovsik ◽  
Michal Stanek ◽  
Adam Dockal ◽  
Martin Reznicek ◽  
Lenka Hylova
Keyword(s):  
Mechanical Properties ◽  
Surface Properties ◽  
Three Dimensional ◽  
Indentation Test ◽  
Optimal Dose ◽  
Indentation Hardness ◽  
Indentation Modulus ◽  
Deformation Work ◽  
Correct Function ◽  
Economical Aspect

Surface properties are important aspect for correct function of construction (technical) parts. By improving mechanical properties of surface, an increase of abrasion resistance and wear resistance is reached. Longevity and economical aspect have an important role in final useful properties of product. Measurement of surface properties was done by ultra-nanoindentation technique (UNHT3), this is the best tool available right now, this technique is based on instrumented testing. Surface properties were modified by ionized radiation, that caused the creation of crosslinked structure in polyethylene. During radiation a three dimensional network is created, that improves final properties of product such as: hardness, elasticity modulus, thermal stability, etc. During ionized radiation there are two actions that take place at the same time, crosslinking and degradation. Goal of this paper is to consider how radiation intensity affects surface properties (indentation hardness, indentation modulus, deformation work, etc.) Another goal of this paper is to find out the optimal dose of radiation, that will cause more three dimensional crosslinking and less degradation as degradation causes decrease in mechanical properties.

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