Structural and Ultrastructural Study, at Ovariectomised Female Rats, and their Reactivity to the Administration of Injectable Oestrogens

2016 ◽  
Vol 695 ◽  
pp. 252-259
Author(s):  
Mocan- Hognogi Radu Florin ◽  
Costin Nicolae ◽  
Constantin Craciun ◽  
Malutan Andrei ◽  
Trif Ioana ◽  
...  
Keyword(s):  
Hormone Replacement ◽  
Basal Layer ◽  
Vaginal Epithelium ◽  
Female Rats ◽  
Control Group ◽  
Average Weight ◽  
Oestrogen Treatment ◽  
Thin Sections ◽  
White Rats ◽  
Group 2

The purpose of this study has been to identify and emphasise structural and ultrastructural modifications occurring in the vaginal epithelium in ovariectomised female rats, as well as their reactivity to the administration of injectable oestrogens. 30 female Wistar white rats have been used, with an average weight of 200 g, distributed as follows: group 1, control group, with no treatment or intervention whatsoever, group 2, menopausal, operated group, with no treatment, and groups 3, 4 and 5, operated, to which oestrogenic treatment was administered. 15 days after surgery, the hormone replacement therapy with injectable oestrogens was initiated (Estradiol, Estradurin, Sintofolin), with a dosage of 0.2 mg/rat/day, and after 14 days of treatment, all animals were sacrificed and biopsies were taken from vaginal epithelium, after which the samples were processed in accordance with optical microscopy techniques (the semi-thin section technique) and transmission electron microscopy (TEM). In group 2, the vaginal epithelium was congested and exhibited relatively numerous invaginations of the mucosa and irregularities of the surface of the epithelium, with a decrease in the number of cells forming every layer, including the ones in the basal layer, which could no longer ensure the regeneration of all cells lost at the level of the epithelium of the superficial layer. In groups 3, 4 and 5, compared to group 2, ovariectomised, the semi-thin sections obtained revealed hyperplasia of all cellular layers of the vaginal epithelium. Ultrastructural investigations confirm the results of the structural study based on semi-thin sections, as the injectable oestrogen treatment has protected and rebuilt the structure of the vaginal epithelium, the chorion and the muscular layers affected by ovariectomy. Our study has shown that experimentally induced menopause has caused significant modifications, expressed polymorphically with alterations to various degrees of ultrastructural visualisation at the level of the entire vaginal epithelium. In all groups treated with oestrogens, it has become apparent that the vaginal epithelium is hyperplased, with the vast majority of its cells having a normal or almost normal aspect, and an isolated presence of structures that have not been completely rebuilt.

scholarly journals Influence of toxoplasma gondii antigens on features of immune status at prenatal and early postnatal periods: experimental study

2014 ◽  
Vol 95 (3) ◽  
pp. 398-401
Author(s):  
T F Sokolova ◽  
D G Novikov ◽  
A V Indutny
Keyword(s):  
Experimental Study ◽  
Toxoplasma Gondii ◽  
Blood Count ◽  
Immune Status ◽  
Perinatal Period ◽  
Female Rats ◽  
Control Group ◽  
White Rats ◽  
Group 2 ◽  
Group 1

Aim. To detect the features of immune status at prenatal and early postnatal periods under the influence of Toxoplasma gondii antigens. Methods. The experimental study was performed on Wistar white rats, who were the offspring of the female rats who were sensitized by T. gondii corpuscular antigen during the III trimester of pregnancy - group 1 (n=96) and on animals who were administered T. gondii corpuscular antigen at firs day of life - group 2 (n=103). Control group consisted of intact rats. Common blood test, levels of antibody-forming cells and CD3+ cells were assessed at 60 day after birth. Results. Neutrophil blood count was 1.9 times higher in the group 1 rats compared to group 2. Eosinophil blood count was 1.4 times lower in the group 1 compared to control group (р=0.01), and 2 times lower in the group 2 compared to control group (р=0.002). At the same time, lymphocyte count was comparable in the group 1 rats and control group, while it was 1.4 times lower in the group 2 compared to control group (р=0.04). Together with that, there was a reduction of CD3+ cells and antibody-forming cells in blood and spleen, which was more marked in the 2nd group. Lymphoadenopathy, thymus dysgenesia, reduced blood and spleen T-cells levels, low humeral immunity were found in Wistar white rats, who were the offspring of the female rats who were sensitized by T. gondii corpuscular antigen during the III trimester of pregnancy. Conclusion. The influence T. gondii corpuscular antigen on rats during perinatal period results in secondary immunodeficiency, persisting at 60 day of life.

scholarly journals The Effect of Zinc and Melatonin Supplementation on Lipid Peroxidation of Various Tissues of Rats with by DMBA-Induced Breast Cancer

2020 ◽  
Vol 11 (1) ◽  
pp. 7580-7588
Keyword(s):  
Breast Cancer ◽  
Lipid Peroxidation ◽  
Kidney Tissue ◽  
Female Rats ◽  
Control Group ◽  
Tissue Samples ◽  
Cancer Group ◽  
Group 5 ◽  
Group 2 ◽  
Group Control Group

The purpose of this study was to investigate the effects of zinc and melatonin administration on lipid peroxidation in various tissues in DMBA-induced breast cancer in female rats. A total of 42 recently weaned Wistar rats were divided into 5 groups in the study: Group 1 (Control), Group 2 (DMBA Control), Group 3 (DMBA+Zinc), Group 4 (DMBA+Melatonin), Group 5 (DMBA+Melatonin, and Zinc). MDA (malondialdehyde) and GSH (glutathione) levels were determined via the spectrophotometric method in the lung, liver, spleen, pancreas, and kidney tissue samples taken from experimental animals. The highest lung, liver, spleen, pancreas, and kidney tissue MDA levels were obtained in the DMBA-induced breast cancer group control group (G2) (p<0.05). MDA levels in DMBA+Zinc (G3), DMBA+Melatonin (G4), and DMBA+Melatonin and Zinc (G5) were significantly lower than group 2 (p<0.05). Similarly, lung, liver, spleen, pancreas, and kidney tissue GSH levels of DMBA+Zinc (G3), DMBA+Melatonin (G4), and DMBA+Melatonin and Zinc (G5) were significantly higher than that of Group 2 (p<0.05). The findings of the study indicated that increased lung, liver, spleen, pancreas, and kidney damage in DMBA-induced breast cancer is suppressed with the supplementation of zinc, melatonin, and combined zinc and melatonin.

scholarly journals Evaluation of Some Male and Female Rats’ Reproductive Hormones Following Administration of Aspartame With or Without Vitamin C or E

2021 ◽  
Vol 45 (2) ◽  
pp. 14-20
Author(s):  
Omar H Azeez
Keyword(s):  
Vitamin C ◽  
Reproductive Hormones ◽  
Female Rats ◽  
Control Group ◽  
Male And Female ◽  
Group 4 ◽  
Male And Female Rats ◽  
Group 3 ◽  
Group 2

Aspartame (ASP) is a sugar substitute. Its use rose because it has been demonstrated to have deleterious effects after being metabolized. In the presence of antioxidant vitamins C or E, the effects of ASP on reproductive hormones of adult male and female Albino Wister rats were investigated. A total of eighty male and female rats were used in this study. The rats were divided into four groups: group 1, received no treatment; group 2, received ASP at 40 mg/kg BW; group 3, received ASP at 40 mg/kg BW with vitamin C at 150 mg/kg BW; and group 4, received ASP at 40 mg/kg BW and vitamin E at 100 mg/kg BW. All treatments were given orally by gavage needle once daily for consecutive 90 days. The levels of estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone hormone (TH) were measured after 90 days in blood plasma. In comparison with the control group, ASP treatment resulted in lower levels of E2, FSH, and LH in male and female rats. When the antioxidants vitamin C or E was given, the effects of ASP were reversed, and the levels of E2, LH, and FSH were increased. The testosterone hormone was likewise significantly increased by ASP, but testosterone hormone concentrations were decreased by vitamin C or E treatments. Long-term ASP consumption caused interfering with testicular and ovarian hormonal activity, while vitamins C and E on the other hand, overcome longstanding consumption ASP's effects.

Investigation of TAp63 gene Expression and Follicle Count Using Melatonin in Cisplatin-induced Ovarian Toxicity

2020 ◽  
Author(s):  
Hacı Öztürk Şahin ◽  
Mehmet Nuri Duran ◽  
Fatma Sılan ◽  
Ece Sılan ◽  
Duygu Sıddıkoglu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Female Rats ◽  
Histological Evaluation ◽  
Saline Control ◽  
Control Group ◽  
Ovarian Toxicity ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Abstract Background: Premature ovarian failure is among the most important side effects of chemotherapy during reproductive period. Preserving ovarian function is gradually gaining importance during oncologic treatment. The present study aims to investigate the potential of melatonin to protect from cisplatin-induced ovarian toxicity in rats. Twenty nine female rats were divided to three groups: Saline control group (Group 1), cisplatin group (Group 2), and cisplatin+melatonin group (Group 3). While the rats in Groups 2 and 3 were administered 5 mg/kg single dose of cisplatin via intra-peritoneal (IP) route, the rats in Group 3 were started on melatonin (20 mg/kg IP) before cisplatin administration and continued during 3 consecutive days. Ovaries were removed one week after cisplatin administration in all groups. Blood samples were obtained before the rats were decapited. Histological evaluation, follicle count, and classification were performed. TAp63 mRNA expression was evaluated using mRNA extraction and real-time polymerase chain reaction (PCR) method. Serum estradiol (E2) and anti-mullerian hormone (AMH) values were measured with enzyme immune-assay technology. Results: While primordial follicles were seen to decrease in Group 2 as compared to Group 1 (p:0.023), primordial follicle count was observed to be preserved significantly in melatonin group as compared to Group 2 (p:0.047). Moreover, cisplatin-induced histo-pathological morphology was preserved in favor of normal histology in melatonin group. A significant difference was not observed between groups with regard to mean serum AMH and E2 values (p:0.102 and p:0.411, respectively). While TAp63 gene expression significantly increased in Group 2 as compared to control group (p:0.001), we did not detect a statistically significant difference in cisplatin+melatonin group, although gene expression decreased (p:0.34). Conclusion: We conclude that concurrent administration of melatonin and cisplatin may protect from ovarian damage.

scholarly journals The Role of Hippocampal Estradiol Receptor-αin a Perimenopausal Affective Disorders-Like Rat Model and Attenuating of Anxiety by Electroacupuncture

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Xun Wang ◽  
Yongheng Huang ◽  
Shiwen Yuan ◽  
Amin Tamadon ◽  
Shulan Ma ◽  
...  
Keyword(s):  
Affective Disorders ◽  
Hormone Replacement ◽  
Female Rats ◽  
Control Group ◽  
Estradiol Treatment ◽  
Estradiol Receptor ◽  
Plus Maze ◽  
Before And After ◽  
Behavioral Parameters

Hormone replacement therapy is the principal treatment for perimenopausal affective disorders which can cause severe side effects. The present study compared the effects of electroacupuncture (EA) and estradiol treatment on perimenopausal affective disorders at the behavioral and cellular levels. In this randomized experimentalin vivostudy, adult female rats were divided into intact, ovariectomy, chronic unpredictable stress (CUS), and ovariectomy and CUS combination groups. After week 6, all groups were subdivided to three subgroups of control, EA, and estradiol treatment. The behavioral parameters in the open field and the elevated plus maze tests were assessed before and after treatments. Alterations of serum steroid hormones and changes of estradiol receptor-α(ER-α) immunofluorescence neurons in the hippocampus sections were evaluated. EA treatment caused more antianxiety effects than estradiol treatment in CUS group (P<0.05). Notably, estradiol and EA treatments had better significant behavioral effects when the models were not estrogen-deficient. Importantly, within each group, compared to the control group, the numbers of ER-α-positive neurons were significantly larger in EA subgroups. Therefore, EA had antianxiety effects on perimenopausal affective disorders caused by CUS but not by estrogen deficiency and upregulation of hippocampus ER-αneurons may contribute to its mechanism of action.

scholarly journals Impact of lead acetate and sodium and potassium stearates on lipid peroxidation processes in the body of experimental animals

2021 ◽  
Vol 100 (4) ◽  
pp. 406-410
Author(s):  
Olha Ye. Fedoriv ◽  
Alexandra Ye. Kopach ◽  
Nataliia A. Melnyk
Keyword(s):  
Lipid Peroxidation ◽  
Drinking Water ◽  
Lead Acetate ◽  
Sodium Stearate ◽  
The Body ◽  
Female Rats ◽  
White Female ◽  
Control Group ◽  
Diene Conjugates ◽  
Group 2

Introduction. Given the significant prevalence of lead in the environment, research in this area has significant social and economic importance. Lead compounds are characterized by high toxicity and increased ability to cumulate in ecosystems, humans, and animals. Lead enters the human body with food, drinking water, atmospheric air, and smoking. Lead causes pathological changes in the nervous system, blood-forming organs, kidneys, etc. Materials and methods. The experiments were carried out on four groups of white female rats, each included seven animals, weighing 150-200 g. The first group of animals was a control. The second group consumed dechlorinated water from the city water supply, followed by lead acetate. The animals from the third and fourth groups drank the same water with sodium stearate and potassium stearate content in a dose of 1/250 LD50. After the 40th-day of the use of these waters, the animals were orally administered lead acetate at a dose of 7 mg/kg. The levels of lipid peroxidation biomarkers were studied by studying the content of diene conjugates (DC) and malondialdehyde (MDA) in blood serum, liver, and kidney homogenates. Results. The administration of 1/2 acetate LD50 to lead in experimental rats drinking water with stearates was accompanied by a significant increase in the DCs concentration and (MDA) in animals. Higher concentrations of LPO products were observed in the group of animals that consumed water from potassium stearate. Conclusions. 1. With the oral administration of lead acetate against the background of drinking water containing stearates at a dose of 1/250 LD50, an increase in lipid peroxidation indices was noted compared with the control group. 2. Higher concentrations of LPO products were observed in the group of animals consuming water from potassium stearate.

scholarly journals Efek Abelmoschus esculentus terhadap Glukosa Darah, Superoksida Dismutase, dan Malondialdehid Rattus norvegicus Diabetes Melitus dengan Induksi Streptozotocin

2018 ◽  
Vol 7 (2) ◽  
pp. 202
Author(s):  
Olivia Herliani
Keyword(s):  
Blood Glucose ◽  
Treatment Group ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
Abelmoschus Esculentus ◽  
Antioxidant Defenses ◽  
Control Group ◽  
Sod Activity ◽  
White Rats ◽  
Group 2

In diabetes mellitus there is an increase in oxidative stress in which oxidants exceed the antioxidant system resulting from the production of oxidant-antioxidant imbalance and/or limited antioxidant defenses. Abelmoschus esculentus (“okra”) is known as lowering blood glucose vegetable by Indonesian. A. esculentus has a high antioxidant activity that can: 1) lower H2O2 level; 2) decrease the amount of ROS; 3) lower •OH level. This study tried to prove that administration of A. esculentus may decrease fasting blood glucose, serum malondialdehyde, and increase superoxide dismutase activity of diabetic male white rats. This research is a pure experimental research, with randomized post test only control group designs, held in 28 days. Thirty one rats were divided into 4 groups randomly: KN = normal control group given 0.1% Na-CMC solution; KDM = DM control group injected with STZ, followed by 0.1% Na-CMC solution; P1 = treatment group 1 injected with STZ, followed by administration of A. esculentus at a dose of 775 mg/kgBW/day; P2 = treatment group 2 injected with STZ, followed by administration of A. esculentus at a dose of 1550 mg/kgBW/day. The result: administration of A. esculentus at doses of 775 mg/kgBW/day and 1550 mg/kgBW/day can not decrease fasting blood glucose and MDA serum level of diabetic male white rats. Giving A. esculentus at a dose of 1550 mg/kgBW/day may increase the activity of SOD erythrocytes of diabetic male white rats.. Conclusions: A. esculentus has the potential to lower blood glucose and MDA serum levels, also increase erythrocyte SOD activity.

scholarly journals Effects of Tocotrienol and Lovastatin Combination on Osteoblast and Osteoclast Activity in Estrogen-Deficient Osteoporosis

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Saif Abdul-Majeed ◽  
Norazlina Mohamed ◽  
Ima-Nirwana Soelaiman
Keyword(s):  
Bone Formation ◽  
Combined Treatment ◽  
Female Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Hmgcoa Reductase ◽  
Reductase Inhibitors ◽  
Group 4 ◽  
Group 3 ◽  
Group 2

Statins are HMGCoA reductase inhibitors and had been demonstrated to stimulate bone formation in rodents after high oral doses. Observational studies on patients treated with oral statins were varied. Delta-tocotrienol had been found to stimulate the cleavage of HMGCoA reductase and inhibit its activity. Tocotrienols were found to have both catabolic and anabolic effects on bone in different animal models of osteoporosis. The current study aimed to ascertain the effects of delta–tocotrienol and lovastatin combination on biochemical and static bone histomorphometric parameters in a postmenopausal rat model at clinically tolerable doses. 48 Sprague Dawley female rats were randomly divided into 6 groups: (1) baseline control group; (2) sham-operated control group; (3) ovariectomised control group; (4) ovariectomised and 11 mg/kg lovastatin; (5) ovariectomised and 60 mg/kg delta-tocotrienol; (6) ovariectomised and 60 mg/kg delta-tocotrienol + 11 mg/kg lovastatin. These treatments were given daily via oral gavage for 8 weeks. Delta-tocotrienol plus lovastatin treatment significantly increased bone formation and reduced bone resorption compared to the other groups. Therefore, the combined treatment may have synergistic or additive effects and have the potential to be used as an antiosteoporotic agent in patients who are at risk of both osteoporosis and hypercholesterolemia, especially in postmenopausal women.

scholarly journals The influence of injection of depot medroxy progesterone acetate (DMPA) to the gland tissue histology breast on white rats breastfeeding

2018 ◽  
Vol 12 (2) ◽  
pp. 94
Author(s):  
Asmita Dahlan
Keyword(s):  
Treatment Group ◽  
Group Treatment ◽  
Female Rats ◽  
White Female ◽  
Control Group ◽  
Control Group Design ◽  
Pathology Laboratory ◽  
White Rats ◽  
Gland Tissue ◽  
Tissue Histology

<p><em>Depo MedroxyProgesteroneAcetate</em> (DMPA) allegedly have the effect of breast glandular tissue breast-feeding women, and to prove it then conducted research into how the influence of injection of DMPA to the gland tissue histology breast on white female rats breastfeeding.This research is experimental research design with post test only control group design that was implemented starting carried out in laboratories of pharmaceutical Faculty of Science and anatomic pathology laboratory andalas university medical school fields, the total sample 27 white female rats lactating drawn at random by age 2-3 months, weight 160-200 grams, divided into 3 groups consisting of the control group, treatment group 1 (DMPA 150 mg) and treatment group 2 (DMPA 300 mg). DMPA injections done in intra muscular  every 5 days during 4x injections (20 days). Research data were statistically processed using anova test with 95% confidence. Results showed that there are differences in the development of lobulus and asinus in the control group and treatment group.Conclusions of this study is the effect of DMPA injections in female rats breastfeeding on the area and the development of lobular breast lobes as well as breast acini.</p>

scholarly journals Expression of the Sod1 gene under conditions of experimental ethanol intoxication and administration of hepatoprotective drugs

2021 ◽  
pp. 132-137
Author(s):  
G. F. Mukhammadieva ◽  
A. B. Bakirov ◽  
D. O. Karimov ◽  
Ya. V. Valova ◽  
M. M. Ziatdinova ◽  
...  
Keyword(s):  
Rat Liver ◽  
Expression Level ◽  
Control Group ◽  
Reverse Transcription Pcr ◽  
Group 4 ◽  
White Rats ◽  
Group 5 ◽  
Group 2 ◽  
Prophylactic Use ◽  
Group Control Group

The aim of the study was to study the effect of hepatoprotective drugs on the expression of the Sod1 gene in rats with ethanol liver damage.Materials and methods. Male outbred white rats were used in the experiment. Five groups of animals were formed, 14 individuals each. Distilled water was administered to rats of the 1st group (control); Group 2 — ethanol at a dose of 5 g/kg of body weight; Group 3 — ethanol and heptor at a dose of 72 mg/kg; Group 4 — ethanol and mexidol at a dose of 50 mg/kg; Group 5 — ethanol and OMU at a dose of 50 mg/kg. The drugs were administered 1 hour before the introduction of ethanol. 24 and 72 hours after the introduction of ethanol (7 individuals), the animals were decapitated and the liver was removed. The expression level of the Sod1 gene was assessed using real-time reverse transcription PCR.Results. The fold change in Sod1 expression in rat liver after 24 h practically did not change in response to the introduction of ethanol to the animals. A tendency to a slight decrease was observed in relation to changes in the expression of Sod1 with the use of heptor and mexidol, while under the influence of OMU, the expression level increased moderately. After 72 h, the exposure to ethanol was accompanied by a slight decrease in the frequency of expression of the Sod1 gene. A similar trend was observed with respect to changes in Sod1 expression with the use of heptor, mexidol, and OMU.Conclusion. The results obtained indicate that the introduction of both ethanol and the prophylactic use of hepatoprotective drugs did not lead to significant changes in the level of Sod1 gene expression in rat liver. Additional studies are needed to identify the mechanisms of regulation of the antioxidant system, as well as the search for drugs that affect the transcriptional activity of genes.

Sign in / Sign up

Export Citation Format

Share Document