Exercise preserves muscle mass and force in a prostate cancer mouse model

The purpose of this study was to examine the effects of exercise in modulating biomarkers of sarcopenia in a treatment naïve transgenic adenocardinoma of the mouse prostate (TRAMP) model. Thirty TRAMP mice were randomized to either exercise (voluntary wheel running) or no-treatment control group for a period of 20 weeks. During necropsy, gastrocnemius muscles and prostate tumors were harvested and weighed. Gastrocnemius concentrations of myostatin, insulin-like growth factor (IGF)-1 and tumor necrosis factor (TNF)-α were quantified. Exercise mice had greater muscle mass than controls (p=0.04). Myostatin was significantly lower in the exercise group compared to controls (p=0.01). Exercise mice maintained forelimb grip force while control mice had a significaint decrease (p=0.01). No significant difference was observed in pre-post all limb grip strength. Further, forelimb and all limb grip strength was negatively associated with tumor mass (p<0.01).

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What Happens to Adipokines and Cytokine in Overweight and Obese Girls with Central Precocious Puberty When They Exercise and Stop Exercising? A randomized Controlled Trial

10.21203/rs.3.rs-560214/v1 ◽

2021 ◽

Author(s):

Elnaz Shokri ◽

Ali Heidarian pour ◽

Zahra Razavi

Keyword(s):

Precocious Puberty ◽

Training Session ◽

Exercise Group ◽

Trial Registration ◽

Control Group ◽

Blood Samples ◽

Combined Exercise ◽

Tnf Α ◽

Significant Difference ◽

And Control

Abstract Background we investigate the levels of adiponectin, resistin, tumor necrosis factor-alpha (TNF-α) and signs of puberty progression after 12-weeks of combined exercise and 4-weeks of detraining. Methods Thirty overweight and obese girls (age7-9) with precocious puberty who injected Differlin were randomly divided into two groups (exercise and control). At the beginning, blood samples were obtained from all subjects and serum levels of adiponectin, resistin and TNF-α were measured. Exercise group performed 60minutes of combined (aerobic and resistance) exercises 3 times a week for 12-weeks.Control group did not receive any exercise. 48 hours after the last training session and after four weeks of detraining, blood samples were collected in the second and third stage, respectively. Blood samples were collected from the control group in both steps. BMI, cholesterol, triglyceride, signs of puberty progression (bone age, uterine lengths, ovarian volumes), luteinising hormone (LH) and follicle-stimulating hormone (FSH) were measured on all three occasions. Results In the exercise group, adiponectin significantly increased and resistin significantly decreased after 12-weeks. After 4-weeks of detraining, adiponectin significantly decreased and resistin significantly increased. TNF-α levels did not change significantly during the study. There was no significant difference in all of the factors in the control group. Throughout the 16-week study period, the rate of puberty and LH significantly decreased in both exercise and control groups but FSH and LH/FSH significantly decreased in exercise group, alone. Conclusion Combined exercise increased adiponectin and decreased resistin and rate of puberty. After 4-weeks of detraining, these effects diminished but did not disappear. Trial registration: Trial registration: ISRCTN, ISRCTN39938. Registered 24 may 2021 - Retrospectively registered, https://www.isrctn.com/trialist

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Positive effect of combined exercise on adipokines levels and pubertal signs in overweight and obese girls with central precocious puberty

Lipids in Health and Disease ◽

10.1186/s12944-021-01588-5 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Elnaz Shokri ◽

Ali Heidarianpour ◽

Zahra Razavi

Keyword(s):

Precocious Puberty ◽

Repeated Measures ◽

Training Session ◽

User Profile ◽

Exercise Group ◽

Control Group ◽

Ovarian Volume ◽

Combined Exercise ◽

Tnf Α ◽

Significant Difference

Abstract Background The prevalence of precocious puberty is increasing. Obesity has been demonstrated to be associated with changes in the adipokine profile and incidence of early puberty in girls. This study assessed the pubertal signs, the levels of adiponectin, resistin, and tumor necrosis factor-alpha (TNF-α) after 12 weeks of combined exercise and 4 weeks of detraining in overweight and obese girls with precocious puberty. Methods Thirty overweight and obese girls (aged 7–9) with precocious puberty, who had received Triptorelin, were randomly divided into two groups (15 exercise and 15 control). Initially, serum levels of adiponectin, resistin, TNF-α, luteinising hormone (LH), and follicle-stimulating hormone (FSH) and the signs of puberty progression (bone age, uterine length, and ovarian volume) were measured. The exercise group performed 60 min of combined (aerobic and resistance) exercise three times/week for 12 weeks. The control group did not receive any exercise. 48 h after the last training session and after 4 weeks of detraining, all research variables were measured (also in the control group). The statistical method used for data analysis was repeated measures ANOVA. Results In the exercise group, adiponectin significantly increased and resistin significantly decreased after 12 weeks. After 4 weeks of detraining, adiponectin significantly decreased, but resistin significantly increased. TNF-α levels did not change significantly during the study. There was no significant difference in all of the factors in the control group. Throughout the 16-week study period, the rate of puberty and LH significantly decreased in both exercise and control groups, but FSH, LH/FSH and ovarian volume significantly decreased in the exercise group alone (P<0.05). Conclusions Combined exercise increased adiponectin and decreased resistin and the rate of puberty. However, after 4 weeks of detraining, these effects diminished but did not disappear. Trial registration IRCT, IRCT56471. Registered 25 may 2021 - Retrospectively registered, https://fa.irct.ir/user/profile

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PO-241 Effects of Aerobic Exercise on Activation of Neuroglia Cell and Expression of Inflammatory Factors in Aging Rats

Exercise Biochemistry Review ◽

10.14428/ebr.v1i5.10893 ◽

2018 ◽

Vol 1 (5) ◽

Author(s):

Yaya Xiong ◽

Jia Jia ◽

Xuanming Hao

Keyword(s):

Aerobic Exercise ◽

Exercise Intervention ◽

Exercise Group ◽

Inflammatory Factors ◽

Control Group ◽

Sprague Dawley ◽

Tnf Α ◽

Significant Difference ◽

Group A ◽

Aging Models

Objective In recent years, aerobic exercise has been considered as a method of anti-aging. The aging mechanism in nervous system is closely related to increased activation of neuroglia cells and progressing neuroinflammation with aging.This study tried to shed some light on relationship between chronic inflammation accumulation and aerobic exercise so as to understand how exercise intervened nervous aging through inflammation. In this study, we used D- galactose aging models, applying two intervention ways: aerobic exercise during aging process, after that, to explore the changes of astrocytes, microglia and TNF-α expression. This study aims to provide certain evidence that sport plays a role in anti-aging. Methods Fifty male Sprague-Dawley rats at age of eight weeks, are randomly divided into five groups: control group(A), Aging group(S), Aging while exercising group(YS), After aging Quiet group(SA), After aging exercise group(SY). The aging groups were subcutaneously injected with D- galactose 150mg / kg / d for six weeks. Group YS do swimming exercise three times a week,once 60 minutes in the first six weeks. SY had same exercise intervention in the 7-12 week after injection. We took the hippocampus of rats in A, S, YS at the end of the sixth week, and of those in SA, SY at the end of twelfth week. tested the expression of GFAP, CD11b and TNF-α by immunohistochemical staining. Results 1.GFAP immunohistochemistry: Compared with group A,The expression of GFAP in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of GFAP in group YS significantly decreased by 20.5% (P<0.01). There is no significant difference between group SA and SY(P > 0.05). 2.CD11b immunohistochemistry: Compared with group A, The expression of CD11b in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of CD11b in group YS decreased by 18.4% (P < 0.05).There is no significant difference between group SA and SY(P > 0.05). 3.TNF-α Immunohistochemistry: Compared with group A,The expression of TNF-α in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of TNF-α in group YS decreased by 30.1% (P < 0.01).There is no significant difference between group SA and SY(P > 0.05). Conclusions Exercise could effectively delay aging progression through improving neuroinflammation, but hard to reverse it, so the earlier in age to exercise, the better to delay aging.

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Inhibition of Leukocyte Entry into the Brain by the Selectin Blocker Fucoidin Decreases Interleukin-1 (IL-1) Levels but Increases IL-8 Levels in Cerebrospinal Fluid during Experimental Pneumococcal Meningitis in Rabbits

Infection and Immunity ◽

10.1128/iai.68.6.3153-3157.2000 ◽

2000 ◽

Vol 68 (6) ◽

pp. 3153-3157 ◽

Cited By ~ 47

Author(s):

Christian Østergaard ◽

Runa Vavia Yieng-Kow ◽

Thomas Benfield ◽

Niels Frimodt-Møller ◽

Frank Espersen ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Pneumococcal Meningitis ◽

Interleukin 1 ◽

Treated Group ◽

Control Group ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Significant Difference ◽

The Brain ◽

Experimental Pneumococcal Meningitis

ABSTRACT The polysaccharide fucoidin is a selectin blocker that inhibits leukocyte recruitment into the cerebrospinal fluid (CSF) during experimental pneumococcal meningitis. In the present study, the effect of fucoidin treatment on the release of the proinflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin-1 (IL-1), and IL-8 into the CSF was investigated. Rabbits (n = 7) were treated intravenously with 10 mg of fucoidin/kg of body weight every second hour starting 4 h after intracisternal inoculation of ∼106 CFU of Streptococcus pneumoniae type 3 (untreated control group, n = 7). CSF samples were obtained every second hour during a 16-h study period. Treatment with fucoidin caused a consistent and significant decrease in CSF IL-1 levels (in picograms per milliliter) between 12 and 16 h (0 versus 170, 0 versus 526, and 60 versus 1,467, respectively;P < 0.02). A less consistent decrease in CSF TNF-α levels was observed in the fucoidin-treated group, but with no significant difference between the two groups (P > 0.05). In contrast, there was no attenuation in CSF IL-8 levels. Indeed, there was a significant increase in CSF IL-8 levels (in picograms per milliliter) in the fucoidin-treated group at 10 and 12 h (921 versus 574 and 1,397 versus 569, respectively;P < 0.09). In conclusion, our results suggest that blood-derived leukocytes mainly are responsible for the release of IL-1 and to some degree TNF-α into the CSF during pneumococcal meningitis, whereas IL-8 may be produced by local cells within the brain.

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The effect of 10-week swimming and bosu exercises on dynamic balance parameter in 8-10 years old boys

Journal of Human Sciences ◽

10.14687/jhs.v16i3.5727 ◽

2019 ◽

Vol 16 (3) ◽

pp. 807-814

Author(s):

Hilal Kılınç ◽

Hacı Bayram Temur ◽

Hacalet Mollaoğulları

Keyword(s):

Dynamic Equilibrium ◽

Dynamic Balance ◽

Exercise Group ◽

English Summary ◽

Control Group ◽

Swimming Training ◽

Significant Difference ◽

Before And After ◽

Package Program ◽

Post Test

Bu çalışma 8-10 yaş arası erkek çocuklarda yüzme egzersiz ve bosu çalışmalarının dinamik dengeye etkisini incelemek amacıyla yapıldı. Yaşları ortalaması 8,9333±,78492 yıl olan 30 katılımcı veli ve antrenörlerinden gönüllü katılımları ile ilgili aydınlanmış̧ onamları alınarak çalışmaya dahil edildi. Bunların 20' si egzersiz grubu diğer 10'u da kontrol grubudur. Egzersiz grubunun en az 1 yıl yüzme eğitimi almış olmalarına ve haftada 3 gün düzenli olarak yüzme antrenmanı yapıyor olmalarına dikkat edildi. Egzersiz grubu rastgele yöntemle 2 gruba ayrıldı. Birinci gruba yüzme ve bosu çalışmaları, ikinci gruba ise sadece yüzme egzersizleri yaptırıldı. Bu çalışma 10 hafta süreyle uygulandı. Katılımcıların antropometrik ölçümler ile dinamik denge ölçümleri planlanan antrenman programı öncesi ve sonrası olmak üzere iki defa alındı. Çalışma Van Olimpik yüzme havuzunda uygulandı. Verilerin analizinde SPSS 21 paket programı kullanıldı. Analizler sonucunda yüzme eğitimi alan grubun A (Anterior), PM (Posteromedial) ve PL (Posterolateral) ön test - son test ölçüm değerleri arasında anlamlı (p<0,01) fark olduğu saptandı. Yine yüzme ve bosu eğitimi alan grubun PM ve PL ön test - son test ölçüm değerleri arasında anlamlı (p<0,01) fark olduğu belirlendi. Seçilmiş değişkenlerin ön test- son test ölçüm değerleri arasındaki farkların gruplar arasında anlamlı (p<0,05) bir farklılığın olmadığı görüldü. Extended English summary is in the end of Full Text PDF (TURKISH) file. Özet This study was carried out to investigate the effect of swimming exercise and bosu studies on dynamic equilibrium in 8-10 years old boys. They were included in the study by taking their enlightened consent about their voluntary participation from 30 participants parents and coaches with an average age of 8,9333 ±, 78492 years. 20 of them were exercise group and 10 of them were control group. The exercise group had at least 1 year of swimming training and swimming training 3 days a week was paid attention to be doing regularly. The exercise group was randomly divided into two groups. The first group had swimming and bosu exercises and the second group had only swimming exercises. This study was performed for 10 weeks. The participants' anthropometric measurements and dynamic balance measurements were taken twice before and after the planned training program. The work was carried out at the Olympic swimming pool in Van. SPSS 21 package program was used to analyze the data. As a result of the analyzes, it was found that there was a significant difference (p<0.01) between the A (Anterior), PM (Poateromedial) and PL (Poaterolateral) pre-test and post-test measurements of the swimming group. It was determined that there was a significant difference (p <0.01) between the PM and PL pretest - posttest measurements of the group receiving swimming and bosu training. There was no significant difference (p <0.05) between the pre-test and post-test values of the selected variables.

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The Immuno modulatory Changes of Patients with Tuberculosis.

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v9ispl1.1386 ◽

2018 ◽

Vol 9 (SPL1) ◽

Author(s):

Rafal J Al-Saigh ◽

Hussam Al-Humadi

Keyword(s):

Rural Areas ◽

Liver Function Tests ◽

Pulmonary Diseases ◽

Control Group ◽

Chronic Obstructive ◽

Descriptive Data ◽

Tnf Α ◽

Significant Difference ◽

Level Versus ◽

Factor Α

Tuberculosis(TB) is aninfectious disease caused by Mycobacterium tuberculosis. The aim was to investigate the levels of immunomodulatory markers like interluekin-6 (IL-6), tumor necrotizing factor-α (TNF- α),cell differentiation-4 (CD4) and CD8 levels in those patients with active tuberculosis (TB) disease in comparison with control group. 41 Adults diagnosed with TB were included in comparison to 32 healthy individuals at Babylon health center for pulmonary diseases and TB. Descriptive data for patients and control group werecollected by well-trained researcher following a structured questionnaire. In parallel, peripheral blood collected to determine IL-6, TNF- α,CD4 and CD8. Then the assessment for the association between clinical and descriptive data and immunomodulatory markers levels was investigated statistically. The majority of TB patients were males (56%) and 71% were resident in rural areas; 47% of them were living in middle socioeconomic state,moreover,47% of TB cases had diabetes,furthermore,51% had chronic obstructive pulmonary diseases,12% had hypertension and 39% of them had chronic anemia with 47% smokers with no significant difference versus control. Following to that,there was highly increased in IL-6 and TNF-α levels in TB patients versus control (P<0.001),with low CD4 level versus control (P<0.001). While there was no significant change shown in CD8 levels versus control and this might highly be correlated with 30% of abnormal liver function tests among TB patients. A high proportion of TB patients have low CD4 level mostly associated with active disease. Moreover,the increase of IL-6 and TNF-α levels suggests a inverse impact on CD4 level which closely associated with the outcome of the disease.

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Preoperative exercise and recovery after cardiac surgery: a meta-analysis

BMC Cardiovascular Disorders ◽

10.1186/s12872-019-01308-z ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Yu-Ting Zheng ◽

Jiang-Xu Zhang

Keyword(s):

Cardiac Surgery ◽

Meta Analysis ◽

Exercise Group ◽

Control Group ◽

Inclusion Criteria ◽

Blood Cell Count ◽

Bias Analysis ◽

Significant Difference ◽

Preoperative Exercise ◽

Review Manager

Abstract Background To evaluate the association between preoperative exercise and recovery after cardiac surgery. Methods Literature comparing preoperative exercise and the control group for patients receiving cardiac surgery was retrieved in multiple databases. Review Manager 5.2 was adopted for meta-analysis, sensitivity analysis and bias analysis. Results Finally, 6 relevant studies satisfied the inclusion criteria. There was significant difference in length of stay in intensive care unit (ICU) (MD- = 1.35, 95%CI [− 2.64, − 0.06], P = 0.04; P for heterogeneity < 0.0001, I2 = 88%) and physical function after operation (P of heterogeneity = 0.32, I2 = 12%, Z = 9.92, P of over effect< 0.00001). The meta-analysis suggested that there was no significant difference in white blood cell count (WBC) at postoperative day 7 and mental health after operation between the exercise group and the control group. Limited publication bias was observed in this study. Conclusion Preoperative exercise including inhaled muscle training, aerobics, resistance training and stretching could promote recovery after cardiac surgery.

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P187 The significance of netrin-1 level in the clinical activity of ulcerative colitis, its association between TNF-α and IL-6

Journal of Crohn s and Colitis ◽

10.1093/ecco-jcc/jjz203.316 ◽

2020 ◽

Vol 14 (Supplement_1) ◽

pp. S232-S232

Author(s):

H Korkmaz ◽

K Fidan

Keyword(s):

Ulcerative Colitis ◽

Proinflammatory Cytokines ◽

Activity Index ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Clinical Activity ◽

Tnf Α ◽

Significant Difference ◽

And Control ◽

Control Study

Abstract Background In this study, we investigated the importance of netrin-1 levels in ulcerative colitis (UC) in clinical activity of the disease, and its association with other proinflammatory cytokines IL-6 and TNF-α. Methods This study is a type of case–control study. Sixty-seven patients with UC (36 of them activation, 31 of remission) and 50 healthy controls were included in the study. UC patients; ‘Truelove Witts clinical activity index by remission (n = 31), mild activation (n = 21), moderate activation (n = 6) and severe activation (n = 9) were divided into groups. Netrin, IL-6 and TNF-α measurements in plasma samples were performed using enzyme-linked immunosorbent assay kit. Results Between the patient group and the control group; there was a statistically significant difference between netrin-1, IL-6, TNF-α, neutrophil, platelet (p < 0.05 for all). The plasma netrin-1 mean of UC with severe activation group (139.21 ± 48.09 pg/ml) was statistically significantly higher than that of the mild activation (p = 0,037), remission group (p = 0,001) and control group(p = 0,011). The plasma netrin-1 mean of UC with moderate activation group was statistically significantly higher than that of the mild activation(p = 0,045) and remission group(p = 0,004). Conclusion Our results reveal that plasma netrin-1 levels have been shown to be associated with UC activation, similar to proinflammatory cytokines such as TNF-α and IL-6, in UC.

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Anti-Inflammatory Effect of Musa acuminata Stem

European Journal of Dentistry ◽

10.1055/s-0040-1709944 ◽

2020 ◽

Vol 14 (02) ◽

pp. 294-298

Author(s):

Maharani Laillyza Apriasari ◽

Selviana Rizky Pramitha ◽

Dewi Puspitasari ◽

Diah Savitri Ernawati

Keyword(s):

Treatment Group ◽

Musa Acuminata ◽

Control Group ◽

Control Group Design ◽

Treatment Groups ◽

Tnf Α ◽

Significant Difference ◽

Anti Inflammatory ◽

Mucosal Wound ◽

Inflammatory Effect

Abstract Objective This study was designed to assess the anti-inflammatory effect of Musa acuminata through the expression of tumor necrosis factor-α (TNF-α) and nuclear factor kappa β (NF-κB) after 3 days of application of Musa acuminata stem extract (MASE) gel on oral mucosal wound. Materials and Methods An experimental study with post-test only control group design was conducted. Twenty male Rattus norvegicus (Wistar) were injured on their left buccal mucosa and treated three times a day with MASE gel of varying concentrations: 0% (as control), MASE 25%, MASE 37.5%, and MASE 50%. On day 3, a biopsy was performed on each mucosal wound for later immunohistochemical analysis for the expressions of TNF-α and NF-κB. Results The highest expression of TNF-α was observed in the control group (13.20 ± 1.79), while the lowest was in the treatment group using 50% MASE (6.40 ± 1.14). Meanwhile the comparison between treatment groups did not highlight any significant difference (p > 0.05). The highest expression of NF-κB was observed in the control group (13.20 ± 1.30), whereas the lowest was in the treatment group using MASE 50% (6.40 ± 1.14). NF-κB was significantly lower in the treatment group using MASE 50% when compared with other treatment groups (p < 0.05). Conclusion Application of MASE on mucosal wound reduces the expression of TNF-α and NF-κB at all concentrations. The anti-inflammatory effect of MASE 50% was the strongest one.

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T-614 inhibits human aortic adventitial fibroblast proliferation and promotes interleukin-8 production in vitro

Vascular ◽

10.1177/1708538119880088 ◽

2019 ◽

Vol 28 (3) ◽

pp. 314-320

Author(s):

Weiping Ci ◽

Tian Wang ◽

Taotao Li ◽

Jin Wan

Keyword(s):

Cell Viability ◽

Vascular Wall ◽

Underlying Mechanism ◽

Interleukin 8 ◽

Control Group ◽

Survival Fraction ◽

Tnf Α ◽

Significant Difference ◽

Factor Α

Objectives The effect and underlying mechanism of T-614 (iguratimod) on Takayasu’s arteritis (TA) are unknown. Here, we report the effects of T-614 on cell proliferation and interleukin-8 (IL-8) production in human aortic adventitial fibroblasts (HAAFs) in vitro and explore its initial benefit in terms of vascular wall inflammation and remodeling for patients with TA. Methods HAAFs were cultured with 0, 5, 50, 100, or 250 μg/ml T-614 in the absence or presence of tumor necrosis factor-α (TNF-α) in vitro. Cell viability was determined by a modified MTT assay. Supernatant IL-8 levels were measured by enzyme-linked immunosorbent assays. Results In the presence of TNF-α, compared to that in the control group, cell viability of HAAFs significantly decreased in the 50, 100, and 250 μg/ml T-614 treatment groups (OD value: P < 0.01, P < 0.001, P < 0.001, respectively; survival fraction (SF): P < 0.05, P < 0.001, P < 0.001, respectively). However, there was no significant difference in cell viability between TNF-α-stimulated and unstimulated groups at the same concentration of T-614. In the absence or presence of TNF-α, T-614 suppressed HAAF cell viability dose-dependently (OD value: r = −0.915, P = 0.000; r = −0.926, P = 0.000, respectively; SF: r = −0.897, P = 0.000; r = −0.885, P = 0.000, respectively). Compared to that in the control group, in the absence of TNF-α, IL-8 levels in the 5 and 100 μg/ml T-614-treated groups were significantly higher ( P < 0.05); in the presence of TNF-α, IL-8 levels in the 5, 50, and 100 μg/ml T-614-treated groups were significantly higher ( P < 0.001, P < 0.001, P < 0.01, respectively). Further, there was a negative correlation between supernatant IL-8 levels and T-614 concentration in groups stimulated with TNF-α ( r = −0.670, P = 0.000), but there was no significant correlation between these parameters in groups that were not stimulated with TNF-α. Conclusions In the absence or presence of TNF-α, T-614 can inhibit HAAF proliferation and promote IL-8 production in vitro; therefore, it could be used to prevent adventitial thickening of the aorta and improve vascular remodeling in inflammatory environments in vitro and might provide a new immunotherapeutic intervention for TA.

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