scholarly journals Fungi associated with african mudfish (Clarias gariepinus, Burchell 1822) in selected fish farms and dams in Zaria and its environs, Kaduna State, Nigeria

2018 ◽  
Vol 10 (1) ◽  
pp. 642
Author(s):  
J.C. Atawodi ◽  
I.A. Yola ◽  
A.H. Kawo ◽  
B.A. Abdullahi
Keyword(s):  
Clarias Gariepinus ◽  
Fish Farms

Incidence and molecular characterization of fungi and yeast isolated from cultured catfish and Nile tilapia

2020 ◽  
Vol 21 (3) ◽  
pp. 61-66
Author(s):  
Ola Hashem ◽  
Viola Zaki ◽  
Rawia Adawy
Keyword(s):  
Molecular Characterization ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Clarias Gariepinus ◽  
Fungal Species ◽  
High Rate ◽  
Fish Farms ◽  
Isolation And Identification ◽  
Penicillium Spp

Objective: To study the incidence and seasonal dynamics of different fungi affected freshwater fishes in Lake Manzala with molecular identification of the isolated fungi. Animals: 300 Nile tilapia (Oreochromis niloticus) and 300 catfish (Clarias gariepinus). Design: Descriptive study. Procedures: Random samples of Oreochromis niloticus (O. niloticus) and Clarias gariepinus (C. gariepinus) were collected from Manzala fish farms. Clinical and postmortem examination of fish was applied. Isolation and identification of different fungi were performed by conventional methods. Furthermore, the molecular characterization of isolated fungi was carried out. Results: C. gariepinus had a higher rate of infection with different fungal species than O. niloticus. Aspergillus spp. (Aspergillus niger and Aspergillus flavus) were the most fungal isolated from the examined fishes, followed by Penicillium spp. and Candida albicans. Aspergillus spp were detected in all seasons with a higher rate in summer and spring. A. flavus, A. niger, Penicillium spp. and C.albicans isolates were amplified from both C. gariepinus and O. niloticus at the specified molecular weight using PCR. Conclusion and clinical relevance: Fungal infection affected the fish showing different external and internal lesions, all species of Aspergillus were found in all seasons with a high rate in, hot seasons, summer and spring. The Prevalence of Penicillium and C. albicans were also reported. All fungal isolates were identified on the phenotypic and molecular bases.

scholarly journals Genetic Characterization Based on Rapd-Pcr in Cultured Strains of Clarias gariepinus (Siluriformes: Clariidae)

2020 ◽  
Vol 4 (2) ◽  
pp. 81-88
Author(s):  
Ola-Oladimeji Folasade Adesola ◽  
Michael Olufemi Awodiran ◽  
Victor Folorunso Olaleye ◽  
Joel Idowu Awopetu
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Clarias Gariepinus ◽  
Distance Matrix ◽  
Group Method ◽  
Fish Farms ◽  
Chain Reaction ◽  
Pair Group ◽  
Breeding Programmes ◽  
Polymerase Chain

The genetic polymorphisms of catfish populations collected from four fish farms in four Southwestern states of Nigeria were determined by Randomly Amplified Polymorphic DNA Polymerase Chain Reaction technique. This was to characterize the strains genetically and provide information on the studied catfish. Following standard procedures, polymerase chain reaction was performed by mixing 15 μl reaction mixture containing FIREPOL® Taq DNA polymerase, MgCl2, dNTPs, distilled water, dyes and glycerol, with 1.0 μl DNA sample of 16-week old C. gariepinus strains using OPA03, OPA04, OPC02, OPB08, OPC11, OPG16, OPG19 and OPA19 primers. A sum of 1708 loci having 3072 bands was amplified in all the samples. The RAPD analysis revealed significant genetic variability (P<0.05) among the four sampled populations. The unweighted pair group method with average (UPGMA) dendrogram based on Nei’s unbiased genetic distance matrix separated the Clarias gariepinus populations into two clades. The first clade was made up of populations from Ibadan and Abeokuta while the second clade consisted of populations from Ado-Ekiti and Ile-Ife. Thus, it is imperative to determine the genetic variation and population structure of the stocks of C. gariepinus in advance before commencing on breeding programmes.

scholarly journals Efficacy of some synthetic antibiotics on Streptococcus pneumoniae and Proteus mirabilis isolated from fish tanks

2020 ◽  
Vol 42 (1) ◽  
Author(s):  
A. A. Idowu ◽  
N. B. Ikenweive ◽  
A. A. Alimi ◽  
A. A. Akinyemi
Keyword(s):  
Proteus Mirabilis ◽  
Clarias Gariepinus ◽  
Fish Farms ◽  
Test Organisms ◽  
Zones Of Inhibition ◽  
Diseased Fish ◽  
Food Fish ◽  
Inhibition Zones ◽  
Concrete Tank ◽  
Fish Tanks

Effects of some synthetic antibiotics on Streptococcus pnemoniae and Proteus mirabilis isolated from cultured Clarias gariepinus, an important food fish raised in a concrete tank was carried out to ascertain their remedies on mortalities of the Clarias gariepinus adult fish. Streptococcus pnemoniae and Proteus mirabilis were isolated from the gill and skin of diseased fish and water samples and identified by standard microbiological methods.The bacteria isolates were tested for sensitivity on ten (10) antibiotics namely Amoxycillin, Gentamicin, Nitrofurantoin, Erythromycine, Augmentin, Tetracycline, Naladoxic acid, Cotrimoxazole, Cloxacillin and Ofloxacin. These organisms reacted differently to the different antibiotics that were used - Ofloxacine, Gentamycin and Erythromycine had highest zones of inhibition of 26mm and 26mm, 24mm and 19mm, 16mm and 17mm respectively around Streptococcus pnemoniae and Proteus mirabilis, but Naladoxic acid, Augmentin, Cloxacillin and Amoxycillin had no inhibition zones around both organisms. It is therefore recommended that Ofloxacine, Gentamycin and Erythromycine can be used to combat S. pnemoniae and P. mirabilis diseases in fish farms because of their high antibacterial potentials against the test organisms.

scholarly journals Monitoring of productive and biochemical parameters of young Clarias gariepinus during feeding of Skretting and Roycher AQUA feeds in experimental conditions

2021 ◽  
Vol 23 (95) ◽  
pp. 29-37
Author(s):  
A. M. Trofymchuk ◽  
V. S. Bitiutskyi ◽  
N. Ye. Grynevych ◽  
O. A. Oleshko ◽  
V. M. Polishchuk ◽  
...  
Keyword(s):  
Clarias Gariepinus ◽  
Feed Ratio ◽  
Fish Farms ◽  
Control Groups ◽  
Experimental Conditions ◽  
Fish Products ◽  
High Feed ◽  
Mathematical Calculation ◽  
Aquaculture Facilities ◽  
Plastic Containers

The article presents the results of monitoring the productive and biochemical indicators of the growth rate of young clary catfish using dry extruded complete feed for fish, which have proven to be best adapted for use at all stages of growing clary catfish, namely: Skretting and Roycher AQUA in experimental conditions. The research was conducted in the educational and experimental laboratories of Bila Tserkva National Agrarian University from 2019 to 2021. For the experiment, we used the young of the African marble clary catfish (Clarias gariepinus), purchased from one of the fish farms in the Odesa region. Clary catfish fry was kept in four rectangular plastic containers for 90 liters of freshwater. The tanks were two-thirds full (60 liters). The water temperature was maintained at 26 °C. For the experiment, 120 fries were used, which were kept in four plastic tanks by the method of analogs of 30 specimens each. Given that the fry differed in weight (for experiment 1 we selected fry with higher weight, and for experiment 2 – with lower weight), they were divided on the principle of analogs into two experimental and two control groups. Based on the results of monitoring in Experiment 1, fish observations, and mathematical calculation, we found that the feed ratio when fed Skretting and Roycher AQUA starter feed is 0.74 and 0.99 %, respectively. Based on the results of monitoring, fish observations, and mathematical calculation, it was found that the feed ratio in experiment 2 when feeding starter feed Skretting and Roycher AQUA is 0.75 and 1.54 %, respectively. The high feed rate for the use of Roycher AQUA can be explained by overuse due to the grinding of the granules. Growing aquaculture facilities in an artificially formed system – production, allows you to get environmentally friendly fish products all year round. This is a very important criterion in modern environmental conditions.

scholarly journals Determination of water quality, trace elements contamination in Oreochomis niloticus and Clarias gariepinus reared in 2 types of ponds and health risk assessment in Cameroon

2020 ◽  
Author(s):  
Isabelle Sandrine Bouelet Ntsama ◽  
Betrand Ayuk Tambe ◽  
Christine Fernande Nyangono Biyegue ◽  
Guy Pouokam ◽  
Hélène Claudia Mentho ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Trace Elements ◽  
Health Risk ◽  
Clarias Gariepinus ◽  
Emission Spectrometry ◽  
Fish Farms ◽  
Farmed Fish ◽  
Earthen Ponds ◽  
Cobalt And Nickel

Abstract Background: Fish farming in Cameroon is growing very rapidly and fish available in the markets is mostly coming from fish farms, but domestic fish production is still low to meet demand. Intensification of production could lead to the occurrence of various types of contaminants that can affect the sanitary quality of farmed fish and consumer health. This study aimed to assess the quality of farmed fish collected in fish farms located in four regions of Cameroon (Center, South, West, Littoral).Results: Concentrations of arsenic, cadmium, lead, chromium, cobalt, and nickel were determined in pond water and muscles of Oreochromis niloticus and Clarias gariepinus raised in concrete and earthen ponds to evaluate health risk for consumers. Trace elements were determined using Inductively Coupled Plasma Optical Emission spectrometry (ICP-OES). Heavy metals in water for concrete and earthen ponds shows there are no significant differences (p<0.05) between the rearing systems except for cobalt and nickel. Heavy metals concentrations in water were lower when compared to the WHO recommended limits except for cadmium in all the ponds. For concrete ponds, heavy metal concentrations decreased in water in the sequence of Cd > As > Co > Ni > Cr > Pb. For earthen ponds the concentrations decreased as Cd > Co > As > Ni > Cr > Pb.Conclusion: Concentrations recorded in the muscles of the 2 species were above the safety limits recommended by FAO/WHO. The target hazard quotient of As was highest compared to other metals and higher than the acceptable limits according to US Environmental Protection Agency guidelines in all fish species. the level of each investigated metal, highlighted a very low health risk for consumers.

scholarly journals Occurrence of multi-drug resistant Enterobacteriaceae in cultured Clarias gariepinus (African catfish) in Kano metropolis, Nigeria

2021 ◽  
Vol 19 (2) ◽  
pp. 106-111
Author(s):  
M.D. Usman ◽  
A.M. Wakawa ◽  
A. Musa ◽  
K.H. Ahmad ◽  
A. Isiaku
Keyword(s):  
Drug Resistance ◽  
Antimicrobial Susceptibility ◽  
Clarias Gariepinus ◽  
Multi Drug Resistance ◽  
African Catfish ◽  
Drug Resistant ◽  
Fish Farms ◽  
Salmonella Spp ◽  
E Coli ◽  
Klebsiella Spp

Multi-drug resistant Enterobacteriaceae were isolated from cultured African catfish (Clarias gariepinus) from ten different fish farms located in Kano Metropolis, Nigeria using conventional methods of bacterial isolation, phenotypic characterization and antimicrobial susceptibility test. This study seeks to document the occurrence of Enterobacteriaceae isolates in cultured African catfish from ten registered fish farms, determine possible resistance to some antimicrobials and the fish safety for human consumption. Isolation and identification of microorganisms were carried out based on the standard procedures and antimicrobial susceptibility to 8 commonly used antimicrobials were conducted using the Kirby-Bauer disc diffusion method. Out of the 400 fish liver sampled, 370 (92.5%) were positive for Enterobacteriaceae isolates, these included 277 (69.25%) E. coli, 13 (3.25%) Salmonella spp, 36 (9%) Klebsiella spp, 21 (5.25%) Proteus spp and 23 (5.75%) Enterobacter spp. The prevalence of the multi-drug resistance was 97.5% for E. coli, 100% for Salmonella spp, 100% for Klebsiella spp, 90.5% for Proteus spp and 82.6% for Enterobacter spp. This study establishes the presence of some Enterobacteriaceae and the development of multi-drug resistance by these microorganisms. More studies like molecular characterization need to be carried out to determine the resistant genes in these organisms, also to assess antimicrobial use among fish farmers and the drug residue levels in the edible tissues of cultured African catfish in Kano Metropolis, Nigeria.

scholarly journals EVALUATION OF THE IMPACT OF WASTEWATER GENERATED FROM CATFISH PONDS ON THE QUALITY OF SOIL IN LAGOS, NIGERIA

2017 ◽  
Vol 16 (1) ◽  
pp. 52-60
Author(s):  
O. E. OMOFUNMI ◽  
J. .K. ADEWUMI ◽  
A .F. ADISA ◽  
S. O. ALEGBELEYE
Keyword(s):  
Crop Production ◽  
Clarias Gariepinus ◽  
Fish Farms ◽  
Critical Range ◽  
Catfish Ponds ◽  
Physical And Chemical ◽  
The Impact ◽  
Site Control ◽  
Water Holding

The study was performed to examine catfish (Clarias gariepinus) effluents on the quality of soil in La-gos State, Nigeria. Five fish farms with highest stock density were selected for evaluation. The soil sampling was collected at 10 metres apart before the effluent discharged site; at the effluent dis-charged site; 10 metres after the effluent discharged site and Non-effluent discharged site (control) denoted as SA, SB, SC and SD respectively. Analysis of the required soil physical and chemical prop-erties were performed at 5 cm depth from 0 – 20 cm. Results showed that the effluents discharged site and Non-effluents discharged site indicated that they contained Temperature (26.5±0.1, 27.5±0.1 oC), pH ( 6.7±0.1, 6.2±0.1), Water Holding Capacity (WHC) (36.4±2.1, 21.4±1.2%), Organic carbon (10.8±0.1, 7.4±0.1 mg/kg), TN (26.4±2.2, 22.4±2.1 mg/kg), TP (7.3±0.1, 6.1±0.1 mg/kg), Potassium (3.4±0.1, 3.1±0.1 mg/kg), Calcium (9.5±0.1, 5.9±0.1 mg/kg), Sodium (1.6±0.1, 0.9±0.1 mg/kg), mag-nesium (8.8±0.1, 7.2±0.1 mg/kg), Zinc (3.3±0.1, 3.0±0.1 mg/kg), Iron (58.7±4.2, 55.8±3.2 mg/kg) and Manganese (23.6±2.3, 21.1±2.2 mg/kg) respectively and were significant different (p≥0.05). Both soil nutrients at the immediate environment of effluents discharged site were within critical range of soil fertility for arable crop production.

scholarly journals Sex distributions and growth response in different populations of catfish (Clarias gariepinus)

2020 ◽  
Vol 47 (3) ◽  
pp. 162-172
Author(s):  
I. Abdulraheem
Keyword(s):  
Growth Rate ◽  
Clarias Gariepinus ◽  
Fish Farms ◽  
Daily Growth ◽  
Female Ratio ◽  
Percentage Survival ◽  
Significant Difference ◽  
Final Weight ◽  
Different Populations ◽  
Number Of Males

This study evaluated the gender ratio in Clarias gariepinus production from five different populations. 300 Juveniles fish each were sourced from five (5) fish farms. The fish were grouped based on the farms into five populations (i.e. treatments) and were replicated thrice with each replicate stocked with 100 fish in a square shaped experimental plastic tanks of 1m x 1m x 1m. Data obtained from the experiment were subjected to one-way analysis of variance. Significant differences (p<0.05) were recorded in the values of final weight, mean weight gain, specific growth rate, percentage survival and daily growth rate. The male-female ratio showed that the females were more in number compared to their male counterpart. There was no significant difference in the number of males, number of females and weight of males across the populations. The female C. gariepinus in population 5 had the highest value (246.41±2.73g) of final weight while those in population 3 had the least value (205.95±13.17g) of weight. The fish in population 2 displayed a better growth curve compared to other populations while fish in population 3 displayed the least curve. The curve revealed that the fish grows at almost the same rate from day 0 to day 42 after which their growth pattern changed. This study showed that the number of female fish in any production outweighs the number of males.

scholarly journals Mortality of Clarias gariepinus caused by Aeromonas caviae and nitrite toxicity in a fish farm

2021 ◽  
Vol 19 (2) ◽  
pp. 138-144
Author(s):  
O.O. Oladele ◽  
N.O. Ameji ◽  
G.Y. Gurumyen ◽  
W.A. Adanu ◽  
T.T. Kolade ◽  
...  
Keyword(s):  
Water Samples ◽  
Pathogenic Bacteria ◽  
Clarias Gariepinus ◽  
Fish Farming ◽  
Fish Pond ◽  
African Catfish ◽  
Fish Farms ◽  
Commercial Fish ◽  
Aeromonas Caviae ◽  
Nitrite Toxicity

In Nigeria, farming of freshwater fish, mainly that of Clarias gariepinus has gained prominence as means of improved protein supply and livelihood. Many farmers suffer untold losses in their bid to make a living from commercial fish production. Among the major causes of infectious disease outbreaks in fish farms are pathogenic bacteria of the genus Aeromonas. This is a case report of outbreak of Aeromonas caviae infection complicated by nitrite toxicity in Clarias gariepinus. Carcasses of 17 African catfish juveniles and two water samples were presented to the poultry and fish clinic of the Veterinary Teaching Hospital, University of Jos for investigation. The fishes had been on medication (Fish cure antibiotic +®) for over 5 days with cumulative mortality of 230 out of 2,500 fishes (9.2 %). Necropsy was conducted and samples were taken for microbial analysis and histopathology while water samples were subjected to chemical analysis. Gross lesions were broken barbels, cutaneous depigmentation, branchial pallor, ascites, renal and splenic congestion. Histologically, there was dissociation of hepatic cords, necrosis of hepatocytes with vacuolation and mononuclear cellular infiltrations. Also, there was renal tubular epithelial necrosis with mononuclear and heterophilic infiltration while micro abscesses were observed in the brain. Marked Zenker’s necrosis and edema were seen in the skeletal muscles. Organism isolated from livers and kidneys was identified as Aeromonas caviae and was susceptible to enrofloxacin, furaltadone and florfenicol. Nitrite in fish pond water was 1mg/L. Bath medication with enrofloxacin at 33mg/litre of water for 6 hours treatment per day was done to reduce the mortality. The farmer was advised to change the source of water for the fish pond immediately. It is recommended that to avoid losses in fish farming, farmers should check the quality of water intended for use on fish farms and ensure early health check on fingerlings purchased for rearing.

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