scholarly journals Phenotypic and molecular variability of maize (Zea mays L.) Induced with X-ray

2021 ◽  
Vol 18 (2) ◽  
pp. 1-25
Author(s):  
Odunayo Joseph Olawuyi ◽  
David Franklin Igata ◽  
Akinlolu Olalekan Akanmu ◽  
Abeeb Abiodun Azeez

Ten genotypes of maize collected from National Center for Genetic Resources and Biotechnology (NACGRAB) were induced with X-ray for morphological and molecular assessment. The experimental design was complete randomized design with four replicates. Morphological and molecular statistical analyses of treated genotypes were conducted using SAS and Power Maker Packages, respectively while dendrogram was generated using Jaccards similarity coefficient using Unweighted Paired Group Method and Arithmetic Averages (UPGMA). The study revealed significant difference which is an indication of genetic variation of characters in treated maize. Genotype DTSR-Wco performed best in plant height (62.35 cm), leaf length (62.35 cm), number of leaves (3.15), leaf width (7.55 cm) and dry leaf biomass (0.24 g). X-ray at 90 Kv/mass, 95 Kv/mass and 100 Kv/mass decreased plant heights to 54.25cm, 53.87cm and 54.10cm respectively compared to Control. Heritability estimate was greater than 70% for all characters evaluated. Genotype TZM 1551 at 0 Kv/mass yielded the highest concentration of DNA at 2841.60 ng/ul and the highest genomic DNA concentration was obtained at 95 Kv/mass for TZM 132 with 1.91%. Primer BMC 1755 was most polymorphic with 58.77% in treated maize genotypes. The plant height was strongly correlated with leaf length (r=0.9), leaf width (r=0.76) and number of leaves (r=0.77). Principal component analysis showed close relationship between plant height (-0.03) and leaf length (0.05) compared with leaf width (-0.67) and number of leaves (0.69). Dry shoot biomass (0.05) was closely related to dry root biomass (-0.03) and dry leaf biomass (-0.04).

2017 ◽  
Vol 62 (2) ◽  
pp. 115-131
Author(s):  
David Animasaun ◽  
Joseph Morakinyo ◽  
Rama Krishnamurthy ◽  
Oba Mustapha

The study assessed the genetic diversity of pearl millet accessions grown in Nigeria and India based on morpho-agronomic traits in order to identify genotypes with superior characters which could be utilized in breeding programmes. Twenty-four pearl millet accessions were grown and evaluated for agronomic and morphological traits during the dry and wet seasons of 2015-2016. Data collected on the accessions using standard descriptors were analysed statistically. IP22281 had the highest mean plant height (108.90 cm) while NGB00531 recorded the lowest (61.02 cm). Significant intra-specific variation existed in number of leaves per plant, leaf length, leaf width, number of nodes and internode length, however, stem girth was similar for the accessions. Tillering was generally poor with the highest value (1.60 tillers per plant) found in NGB00531. A significant positive correlation occurred between plant height, number of leaves, leaf length and leaf width. Panicles emerged between 44 and 56 days and NGB00548 had the shortest maturity time. Also, panicle length and peduncle diameter varied significantly for the accessions. The highest grain yield and 1000-grain weight were recorded in NGB00616 and the lowest yield and weight were recorded in IP22269. The principal component analysis grouped the accessions into four clusters, comprising mixtures of Nigerian and Indian members. Similarly, the dendrogram grouped the accessions into two main groups which were sub-divided into smaller clusters with accessions from Nigeria and India in the same cluster. The study concludes that variations in morpho-agronomic and yield characters among the accessions studied could be harnessed for crop improvement. The clustering pattern of these accessions indicated their genetic relatedness, possibly from the same progenitor, but separation by geographical or ecological isolation mechanisms.


2019 ◽  
Vol 17 (1) ◽  
pp. 175-187
Author(s):  
R Rai ◽  
J Shrestha ◽  
JH Kim

An experiment was carried out using eight double cross F1s (DCF1s) as lines and two testers to obtain sixteen Line ×Tester hybrids for the evaluation of major growth and flowering traits thereby to understand the breeding potentiality of the parental lines. The performance of growth and flowering traits of all twenty-six genotypes were evaluated in a randomized complete block design (RCBD) with three replications. The analysis of variance (ANOVA) showed all the genotypes were significantly different for all agro-morphological traits under study. The estimated general combining ability (GCA) effects showed that parent 5(P5), were superior for plant height, stem diameter, number of leaves, leaf length, days to flowering and attitude of floral axis followed by parent 2(P2) for leaf length, leaf width, days to flowering and attitude of floral axis and parent7 (P7) for number of flower, flower diameter, bud length and attitude for floral axis. Likewise; tester 1 found to be best combiner with significant GCA effect for almost all growth and flowering traits (except number of leaves). The plant height, leaf width, number of flower and attitude of floral axis showed prevailing additive gene action while stem diameter, number of leaves, leaf length, days to flowering, flower diameter and bud length indicated predominance of dominance gene action. We can conclude that parent P5, parent P2 and parent P7 can be used as good mother lines and the tester 1, as good donor for the seed production. SAARC J. Agri., 17(1): 175-187 (2019)


2020 ◽  
Vol 1 (1) ◽  
pp. 40-52
Author(s):  
Arie Hapsani Hasan Basri ◽  
Mahmudah Mahmudah

The main roles of plant growth was nutrition. This research aims to determine the concentration of nutrients that can provide the best growth and yield on various mustard. The research method  used is a factorial Complete Randomized Design (RBD) with three replications and then analyzed using the Least Significant Difference test (LSD) at a level of 5%. The treatment of vegetable types is as follows: S1 = mustard pakchoy, S2 = mustard samhong, S3 = mustard pagoda, S4 = mustard pakchoy mini. Treatment with nutrition is the AB mix fertilizerzs number of leaves solution as follows: N1 = 1000 ppm, N2 = 1200 ppm, N3 = 1400 ppm, N4 = 1600 ppm, N5 = 1800 ppm. The results showed that plant height, number of leaves, leaf width and wet weight of the pagoda and mini pakchoy plants were not significantly different at 1000 ppm, 1200 ppm, 1400 ppm, 1600 ppm and 1800 ppm. Number of leaves of pakchoy significantly different at 1400 ppm. While the number of leaves and leaf width of samhong plants were not significantly different at each treatment, but were significantly different from plant height at 1600 ppm and significantly different from the wet weight at 1400 ppm


Author(s):  
M.I. Adebola, A.E. Esson

In this study, two accessions of Bambara groundnut were exposed to fast neutrons Americium-Beryllium (AmBe) source with flux of 1.5 ×104 ncm-2 s-1for different hours, and planted in a completely randomized block design (RCBD) with three replications to raise M1 generation. The data recorded were percentage germination, plant height, number of leaves, leaf length, leaf width, days to 50% flowering, number of pods per plant, fresh weight of plants, dry weight of plants, dry weight of pods, weight of seeds and100 seeds weight. There was variation in plant height, number of leaves, leaf length, leaf width, Days to 50% flowering, fresh weight of plants, dry weight of plants, dry weight of pods, weight of seeds and100 seeds weight for Black accession. In the Cream accession, days to 50% flowering, number of pods per plant, fresh weight of plants, dry weight of plants, dry weight of pods, weight of seeds and100 seeds weight were significantly different. Treatment at 16 hours performed best for plant height, leaf length, number of leaves, weight of seed per plant and 100 seed weight in Black accession while Cream performed best at 8 hours exposure time. Fast neutron was observed to improve the performance of Bambara groundnut, thus can be used in breeding programme for Bambara groundnut.


2019 ◽  
Vol 50 (2) ◽  
pp. 379-389
Author(s):  
Judit Deme ◽  
Balázs Palla ◽  
Győző Haszonits ◽  
János Csiky ◽  
Kornél Baráth ◽  
...  

Fritillaria meleagris is an endangered Eurasian species with declining populations in most of its distribution area. The signifi cance of the present study is to provide details for improving methods, how age-state structure can be identifi ed in a population. This could help finding the appropriate way of site-management where this species occurs. We investigated two F. meleagris populations in the western part of Hungary (Gyékényes, Tüskeszentpéter). We grouped the recorded plants into five age-state categories: dormant, juvenile, subadult, vegetative- and reproductive adult. We investigated diff erent plant traits such as plant height, number of leaves, leaf width and leaf length. The relative frequencies of leaf numbers differed signifi cantly between the two sites each year. Generally, the majority of the flowering plants had 5-6 leaves at both sites. Only plant height was positively related to plant reproductive success, but there were no clear trends in leaf width and leaf length. There are considerable diff erences in age-state ratios between our sampling sites. The proportion of reproductive plants was noticeably higher in both years in Tüskeszentpéter. Transition into dormant state was regular from all age groups at both sites.


2020 ◽  
Vol 22 (1) ◽  
pp. 36
Author(s):  
Sulistiawan Purnomo Aji ◽  
Siswadi Siswadi ◽  
Saiful Bahri

This study is entitled Concentration Test of Three Liquid Organic Fertilizer From Agricultural Waste on Growth and Yield of Arugula (Eruca Sativa) to test the concentration of three kinds of liquid organic fertilizer, sugarcane bagasse waste, banana peel and coconut fiber with a concentration of 50 ml/l, 100 ml/l, and, 150 ml/l. This study uses a single Randomized Complete Design (CRD) method consisting of 10 treatments that are repeated 3 times, consisting of K0, K1, K2, K3, K4, K5, K6, K7, K8, K9, the results of this study were analyzed with Duncan's Multiple Range Test (DMRT) at 5%, the parameters observed were: plant height, number of leaves, leaf width, leaf length, root length, fresh weightof biomass, and dry weight of biomass. The results of the study show that: (1) Giving treatment (K3), liquid organic fertilizer of banana peels with a concentration of 150 ml/l, provides interaction with parameters of observation of leaf width, root length and dry weight of biomass arugula plants, but they need to increase the concentration of organic fertilizer liquid to assist the growth process with observations of plant height parameters, number of leaves, leaf length and fresh weigh of biomass. (2) Provision of treatment with 150 ml/l concentration is the best treatment for the parameters of observation of leaf width, length of root and dry weight of biomass, but the administration of 150 ml/l concentration is not sufficient for plant nutrient requirements so there is no interaction with the growth process of plant height, number of leaves, length of leaves and fresh weight of biomass. (3) The highest dry weight of biomass in the treatment of liquid organic fertilizer from banana peel waste with a concentration of 150 ml/l, weighing 4.32 g or an increase of 20% compared to the control.


2021 ◽  
Vol 9 (1) ◽  
pp. 68
Author(s):  
Lukito Hasta Pratopo ◽  
Ahmad Thoriq

Kale and catfish are the most popular vegetables and fish in Indonesia. The production of these two commodities can be done using an aquaponics system. This study aims to determine the growth of water spinach plants and the survival of catfish in the aquaponic system. Plant growth parameters were measured every three days which included plant height, leaf width, leaf length, number of leaves and plant weight after harvest. The development of catfish that was observed every three days included the number of dead catfish and the weight of catfish measured every nine days. The data obtained were then tabulated and processed in graphical form and analyzed descriptively. The results showed The growth rate of kale planted in the aquaponic system includes plant height, leaf length, and leaf width, respectively, 2.51 cm / three days, 0.75 cm / three days and 0.24 cm / three days, while in kale plants (control) 1.30 / three days, 0.5 cm / three days, and 0.12 cm / three days, respectively. The yield of water spinach planted with the aquaponics system weighed 350 grams / pot, while the control water spinach was only 135 grams / pot. During 30 days of maintenance there was an increase in weight of catfish as much as 11.25 grams / head with the survival rate (SR) of catfish seeds by 93%.


ZOOTEC ◽  
2016 ◽  
Vol 37 (1) ◽  
pp. 80
Author(s):  
Susisusanty Imban ◽  
A Rumambi ◽  
S S Malalantang

ABSTRACT   UTILIZATION EFFECT OF CATTLE FECES BOKASHI ON GROWTH OF Kawali SORGHUM VARIETY . The Research about effect of utilization cow manure on growth of sorghum variety kawali has done. The aim of this research is to obtain data and determine the effect of utilization cow manure mixed with EM4 on growth of  sorghum variety kawali. This study was conducted using a completely randomized design (CRD) which consists of 4 treatments and 5 replicates in each treatment. The treatments were divided into plots of the experimental unit without fertilizer (P0), the plot using organic fertilizer of 4 kg/6 m2 (P1), plot using organic fertilizer of 8 kg/6 m2 (P2), and plot using organic fertilizer of 12 kg/6 m2 (P3). The variables measured in this study were plant height, number of leaves and leaf width. The HSD test indicated that at high treatment plants showed that treatments between P3 and P0 and between P3 and P1 were highly significant. However, treatments between P3 and P2 showed no significant difference. In the treatments of advanced test HSD indicated also that number of leaves between P3 and P0 showed highly significant, but between P3 and P1, and between P3 and P2 showed no difference. In the treatment of wide leaves using HSD test showed that treatment between P3 and P0 was highly significant, but those between P3 and P1 and between P3 and P2 showed no difference. Statistical analysis showed that treatment using cattle feces bokashi as the organic fertilizer of 12 kg/ 6 m2 (P3) produced high significant influence on plant height, leaf number, and leaf width. Based on this research it can be concluded that cattle feces bokashi as the organic fertilizer up to 12 kg /plot of 6 m2 produced the best effect on plant height, leaf number, and leaf width of kawali sorghum variety. Key words: Sorgum, growt,  Cattle feces bokashi,EM4.


2019 ◽  
Vol 50 (2) ◽  
pp. 365-378
Author(s):  
Bálint Pacsai ◽  
Éva Szabó ◽  
Éva Biró ◽  
Beáta Gerencsér ◽  
Anita Kuczkó ◽  
...  

Fritillaria meleagris is an endangered Eurasian species with declining populations in most of its distribution area. The signifi cance of the present study is to provide details for improving methods, how age-state structure can be identifi ed in a population. This could help finding the appropriate way of site-management where this species occurs. We investigated two F. meleagris populations in the western part of Hungary (Gyékényes, Tüskeszentpéter). We grouped the recorded plants into five age-state categories: dormant, juvenile, subadult, vegetative- and reproductive adult. We investigated diff erent plant traits such as plant height, number of leaves, leaf width and leaf length. The relative frequencies of leaf numbers differed signifi cantly between the two sites each year. Generally, the majority of the flowering plants had 5-6 leaves at both sites. Only plant height was positively related to plant reproductive success, but there were no clear trends in leaf width and leaf length. There are considerable diff erences in age-state ratios between our sampling sites. The proportion of reproductive plants was noticeably higher in both years in Tüskeszentpéter. Transition into dormant state was regular from all age groups at both sites.


2021 ◽  
Vol 8 (1) ◽  
pp. 66-71
Author(s):  
D Jabborova ◽  
Y Enakiev ◽  
Khurshid Sulaymanov ◽  
Dilbar Kadirova ◽  
Ahmad Ali ◽  
...  

Ginger (Zingiber officinale Roscoe) is an important medicinal crop grown for its aromatic rhizome which is used as a spice, food, flavouring agent and medicine. It has been characterised for its hypoglycemic, hypotensive, antioxidant and antibiotic properties. This study was conducted to determine the impact of plant growth-promoting potential of bacterial strain Bacillus subtilis L2 on plant growth and physiological properties of ginger. The experiment was carried out in randomised block design with three replications in pot experiments. The plants were grown in greenhouse conditions for three months. The results showed that at 8 and 12 weeks after planting (WAP) bacterial inoculation increased plant height, leaf length, number of leaves per plant and leaf width. Inoculation with B. subtilis L2 significantly increased plant height by 16, 20 and 18% compared to control at 4, 8 and 12 WAP. At 8 and 12 WAP, leaf length significantly raised by B. subtilis L2 as compared to uninoculated control. B. subtilis L2 significantly increased the number of leaves per plant and leaf width by 30 and 21% respectively when comparing with non-inoculated plants at 8 WAP. The percentage increase in chlorophyll content resulted from the inoculation with B. subtilis L2 over the control was 10.5%, 15.5% and 18.4% at 4, 8 and 12 WAP respectively. It is concluded that there is a significant positive effect of inoculation with B. subtilis L2 on the growth of ginger. B. subtilis L2 strain can be used as a potential agent or bio-fertiliser for stimulation of ginger growth.


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