scholarly journals Ovulatory response and embryo quality in Katahdin ewes supplemented with palm oil

2016 ◽  
Vol 46 (3) ◽  
pp. 261-268
Author(s):  
S Torres-Zapata ◽  
C Luna-Palomera ◽  
JA Aguilar-Cabrales ◽  
JA Peralta-Torres ◽  
JR Aké-López ◽  
...  
Keyword(s):  
Developmental Stage ◽  
Palm Oil ◽  
Embryo Quality ◽  
Follicle Stimulating Hormone ◽  
Ovarian Activity ◽  
Chi Square ◽  
Stage Of Development ◽  
Quality Grade ◽  
To Receive

The aims of this study were to evaluate the superovulatory response (SR), embryo quality and embryonic development in Katahdin ewes that received a dietary supplement of palm oil (PO). Ten ewes were assigned to receive 35 g palm oil (n = 10, PO3) and 10 to receive 70 g palm oil (n = 10, PO6) for 25 days. On day 0, the ewes were synchronized with 0.3 mg progesterone-releasing devices (CIDR-G®). From days 6 to 9, the donors were superovulated with 200 mg follicle-stimulating hormone (FSH) (Folltropin-V®) at decreasing doses every 12 hours (40 - 40, 30 - 30, 20 - 20 and 10 - 10 mg/ewe). On day 8, the devices were removed and donors were mated twice with fertile rams at 12-h intervals. Seven days after mating, the embryos were recovered and evaluated for their stage of development and quality grade (1 = excellent, 2 = good, 3 = bad and 4 = degenerated). In addition, on days 0, 7 and 15, blood samples were taken to determine cholesterol, triglycerides, insulin and insulin-Iike growth factor-1 (IGF-1) concentrations in serum blood. Using chi-square and t-tests, the superovulatory response (SR), ovulation rate (OR), total embryonic structures (TES), transferable embryos (TE), degenerated embryos (DEs) and developmental stage and quality were evaluated. No significant differences were observed between treatments for SR, OR, TES and TE variables or for cholesterol, triglycerides, insulin and IGF-1 concentrations. There were differences in the numbers of DE, as well as a trend for more homogeneous developmental stage in the embryos of ewes supplemented with PO6, as well as a higher number of quality 1 embryos and lower numbers of quality 3 and 4 embryos. Palm oil supplementation could be a recommendable practice to improve the quality of embryos and homogenous developmental stage (morula and blastocyst).Keywords: Fatty acids, ovarian activity, sheep

scholarly journals Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4257
Author(s):  
Alane Pains Oliveira do Monte ◽  
João Bosco Loiola Filho ◽  
Thais Thatiane Dos Santos Souza ◽  
Mayara De Souza Miranda ◽  
Lívia Correia Magalhães ◽  
...  
Keyword(s):  
Embryo Quality ◽  
Control Group ◽  
Chi Square ◽  
Mass Occurrence ◽  
Chi Square Test ◽  
Vitrification Solution ◽  
Cryoprotectant Solution ◽  
Pellucid Zone

<p>This study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced <em>in vivo</em>. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was performed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from Sucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P &lt; 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality.</p>

Effect of different doses of FSH on superovulation, productionand quality of embryo in North Omani Cattle breed

2017 ◽  
Author(s):  
Y.O. Muhammad Al-Shakaili ◽  
A.M. Hussein ◽  
A.N. Al-Ismaily ◽  
H.H. Al-Alawi
Keyword(s):  
Embryo Quality ◽  
Follicle Stimulating Hormone ◽  
Cattle Breed ◽  
Ovarian Response ◽  
Transfer Program ◽  
Significant Difference ◽  
Embryo Transfer Program ◽  
Fair Poor ◽  
Different Doses

The experiment was conducted to investigate the Ovarian response and embryo quality using different doses of follicle stimulating hormone (FSH-P) in North Omani Cattle breed (NOC) in order to determine the suitable dose. Eighteen donor NOC were divided into three groups (G1, G2, and G3) comprising of six cows in each group and treated with 20 (G1), 25 (G2) and 30 mg FSH (G3). Ovarian response was recorded as development of corpus luteum (CL), unovulated follicles (UOF), unfertilized ova (UFO), recovered embryo (RE) and transferable embryos (TE). Embryos were flushed seven days after the first AI and classified according to the developmental stage and quality. Results showed that, cows superovulated with FSH (G3) increased significantly (P less than 0.05) number of CLs and number of RE (6.83 and 5.33 /flushed cow) compared with the G1 of FSH (3.17 and 2.00/flushed cow) and G2 (4.67 and 3.00/flushed cow). While using FSH in (G2) increased significantly (P less than 0.05) the number of TE (excellent and good) quality, decreased average number of UOF and UFO (2.84, 3.33 and 0.67/flush) compared with FSH G3 (1.83, 5.67 and 1.50/flush). While no significant difference was observed in TE number between G1 and G2. G3 groups producing embryos at early stages of development (4 and 8 cells) and this was undesirable for embryo transfer program as this treatment (30 mg) produced more number of fair, poor and degenerated embryos compared with 20 and 25 mg FSH. Consequently, 25 mg FSH dose increased number of TE, decreased number of UOF and UFO, and that was the suitable dose for superovulation of NOC.

Effects of supplements of phosphorus or phosphorus and protein on the ovarian activity of cows fed native pasture hay

1977 ◽  
Vol 17 (85) ◽  
pp. 207 ◽  
Author(s):  
E Teleni ◽  
BD Siebert ◽  
RM Murray ◽  
CD Nancarrow
Keyword(s):  
Reproductive Activity ◽  
Inorganic Phosphorus ◽  
Protein Supplementation ◽  
Ovarian Activity ◽  
Elevated Plasma ◽  
Wet Season ◽  
Plasma Progesterone ◽  
Native Pasture ◽  
To Receive

Eight cows with calves in pens were fed native tropical pasture hay harvested at intervals throughout the wet season. Four of the cows were supplemented with phosphorus. When pastures matured and declined in quality, calves were weaned and their dams were firstly group-fed hay in yards and later allowed to graze native pasture. One group continued to receive phosphorus supplement and both groups were supplied with protein supplement to offset possible deficiency. During the pen feeding period, none of the cows exhibited oestrus and plasma progesterone declined as the quality of the hay fell; the cows were refractory to treatment with luteolytic prostaglandin. Some four months after the commencement of protein supplementation, two cows from the group receiving phosphorus supplement exhibited oestrus. Three cows from each group had elevated plasma progesterone levels, and these animals all exhibited oestrus following treatment with prostaglandin. Phosphorus supplementation had no significant effect on feed intake, liveweight change or milk production. Blood inorganic phosphorus was significantly increased by treatment but bone phosphorus was not. It was concluded that phosphorus supplementation alone had no significant effect on reproductive activity. If cows received adequate protein, however, oestrus activity was markedly increased whether phosphorus was supplemented or not.

scholarly journals Pesisir cattle superovulation with various dosage of Follicle Stimulating Hormone (FSH) on embrio production

2021 ◽  
Vol 888 (1) ◽  
pp. 012034
Author(s):  
T Afriani ◽  
E Purwati ◽  
J Hellyward ◽  
Jaswandi ◽  
M Mundana ◽  
...  
Keyword(s):  
Experimental Design ◽  
Corpus Luteum ◽  
Analysis Of Variance ◽  
Embryo Quality ◽  
Follicle Stimulating Hormone ◽  
Research Material ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Stimulating Hormone

Abstract This study aimed to determine the use of various doses of FSH hormone, the number of lutheum corpus and the number and quality of embryos produced in the superovulation program in Pesisir cattle. The experimental design was Analysis of Variance (Anova) in a completely randomized design. The research material used 16 cows from Pesisir cattle that had been synchronized with estrous with CIDR implants for 13 days and were injected with the PGF2α hormone after the release of CIDR, then scheduled AI was performed. The dose of FSH hormone was 16 ml, 17 ml, 18 ml and 19 ml, each treatment consisting of 4 replications. The injection of the FSH hormone was carried out for 3 days at a decreased dose. Collection of donor embryos was carried out on days 6 to 8 after the scheduled AI. The parameters observed included superovulation response, number of corpus luteum, number of embryos and quality of embryos. The results showed that the response of Pesisir cattle superovulation was 81.25%. The average number of CL obtained was 13.75 and embryos for Pesisir cattle was 11.25. The results also showed that the quality of the embryos obtained were grade A 19, grade B 18, grade C 7, grade D 1. It can be concluded that the use of various doses of FSH hormone at doses of 16 mg, 17 ml, 18 ml and 19 ml had shown a superovulatory response. The 17 ml dose of FSH hormone showed a large number of embryos and had the best embryo quality.

scholarly journals Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4257
Author(s):  
Alane Pains Oliveira do Monte ◽  
João Bosco Loiola Filho ◽  
Thais Thatiane Dos Santos Souza ◽  
Mayara De Souza Miranda ◽  
Lívia Correia Magalhães ◽  
...  
Keyword(s):  
Embryo Quality ◽  
Control Group ◽  
Chi Square ◽  
Mass Occurrence ◽  
Chi Square Test ◽  
Vitrification Solution ◽  
Cryoprotectant Solution ◽  
Pellucid Zone

This study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced in vivo. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was performed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from Sucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality.

scholarly journals Embryo development and follicular status of Toggenburg does fed urea diet

2011 ◽  
Vol 40 (2) ◽  
pp. 277-285 ◽  
Author(s):  
Lincoln da Silva Amorim ◽  
Ciro Alexandre Alves Torres ◽  
Luiz Gustavo Bruno Siqueira ◽  
Jeferson Ferreira da Fonseca ◽  
José Domingos Guimarães ◽  
...  
Keyword(s):  
Developmental Stage ◽  
Dry Matter ◽  
Developmental Stages ◽  
Production Quality ◽  
Urea Concentration ◽  
Advanced Stage ◽  
Estrus Cycle ◽  
Embryo Recovery ◽  
To Receive

The effects of feeding urea on embryo production, quality and developmental stages and follicular status at the beginning of a superovulatory treatment of Toggenburg does fed urea diet were investigated in this study. Eighteen females were randomly allocated to receive diets with: no urea (control, n=8), and 2.4% urea dietary dry matter (n=10) UDM. The embryo recovery was performed at day 7 or 8 of estrus cycle by transcervical technique and classified according to quality and developmental stage. The follicular status was determined by transrectal ultrasonography at the first day of FSH injection. The number and quality of embryos were not affected by dietary urea concentration. Embryos from does treated with 2.4% UDM were recovered at advanced stage. Urea concentration (2.4%) decreases the number of follicles with less than 5 mm diameter. This follicle class is positively correlated to the number of recovered embryos and to the number and percentage of excellent and good embryos.

HUBUNGAN PERAN FAMILY CAREGIVER TERHADAP QUALITAS HIDUP LANSIA

2019 ◽  
Vol 11 (1) ◽  
pp. 51-56
Author(s):  
RIANI PRADARA JATI ◽  
Sekar Farah Nabila
Keyword(s):  
Quality Of Life ◽  
Family Caregiver ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
The Elderly ◽  
Chi Square ◽  
The Poor ◽  
The Family

  Penempatan peran yang baik bagi Family Caregiver sangatlah membantu lansia dalam meningkatkah qualitas hidupnya, meningkatkan motivasi dalam menjalankan hidup Penelitian ini bertujuan Mengetahui hubungan peran Family Caregiver dalam pemenuhan qualitas hidup bagi lansia di Kelurahan Langenharjo Kabupaten Kendal. DesainPenelitianDeskriptifKorelasional menggunakan pendekatan Krosectional,tehnikSamplingStratified Simple Random Sampling dengan karakteristik heterogen, dari populasi mempunyai hak yang sama untuk diseleksi sebagai sampel teknik undianPengambilan data dengan menggunakan kuesioner yang telah diuji validitas dan reliabilitasnya. Uji statistik Chi-square, dengan taraf signifikasi 5%jumlah sampel pada penelitian ini 70 sampel pada Family Caregiver dari 213 populasi yang ada. Hasil penelitian dari 70 responden didapatkan Peran Family Caregiver tidak baik dengan qualitas hidup tidak baik 33 (47,1%), sedangkan Peran Family Caregiver kurang baik dengan qualitas hidup lansia baik 3 (4,3%). Untuk distribusi Peran Family Caregiver kurang baik dengan qualitas hidup lansia tidak baik sebanyak 6 responden (8,6%) sedangkan untuk distribusi Peran Family Caregiver kurang baik dengan qualitas hidup lansia baik sebanyak 23 responden (32,9%). Terakhir, untuk distribusi Peran Family Caregiver baik dengan qualitas hidup lansia tidak baik didapatkan hasil 2 responden (2, 9%) sedangkan untuk distribusi Peran Family Caregiver baik dengan qualitas hidup lansia baik didapatkan hasil 3 responden (4,3%)Menunjukkan nilai ρ value 0,001 (ρ < 0,05) berarti ada hubungan antara dukungan keluarga dengan kepatuhan lansia dalam keikutsertaan posyandu lansia. Disarankan kepada semua Family Cregiver lansia untuk mampu memahami pentingnya perhatian, dukungan bagi lansia dalammeningkatkan qualitas hidup yang lebih baik bagi lansia.   Kata kunci : Peran family caregiver, qualitas hidup, lansia.   ABSTRACT Placement of a good role for Family Caregiver is very helpful for the elderly to improve their quality of life, increase motivation in living life Research Objective: To know the relationship between the role of Family Caregiver in fulfilling quality of life for the elderly in Langenharjo Village, Kendal Regency. Descriptive Correlational Research Design uses a cross sectional approach, Sampling Stratified Simple Random Sampling technique with heterogeneous characteristics, from the population has the same right to be selected as a sample lottery technique Retrieving data using a questionnaire that has been tested for validity and reliability. Test Chi-square statistics, with a significance level of 5% the number of samples in this study 70 samples on the Family Caregiver from 213 populations. Results of the Study Of 70 respondents found the role of Family Caregiver was not good with poor quality of life 33 (47.1%) , while the role of the Family Caregiver is not good with the quality of life of a good elderly 3 (4.3%). For the distribution of the role of Family Caregiver is not good with the quality of life of the poor family as many as 6 respondents (8.6%) while for the distribution of the Role of Family Caregiver is not good with the quality of life of good elderly as many as 23 respondents (32.9%). Finally, the distribution of the Role of Family Caregiver with good quality of life for the poor is obtained by 2 respondents (2, 9%), while the distribution of the Role of Family Caregiver with good quality of life for the elderly is obtained by 3 respondents (4.3%). 0.001 (ρ <0.05) means that there is a relationship between family support and the compliance of the elderly in the participation of the elderly posyandu. It is recommended to all elderly Cregiver families to be able to understand the importance of attention, support for the elderly in improving the quality of life better for the elderly   Keywords: Role of Family Caregiver, Quality of Life, Elderly

Lesson Study dan Implikasinya Terhadap Pembelajaran Berbasis Kurikulum Tingkat Satuan Pendidikan

2016 ◽  
Vol 1 ◽  
pp. 189-196
Author(s):  
Vian Harsution
Keyword(s):  
Learning Environment ◽  
Learning Process ◽  
Lesson Study ◽  
Learning Difficulties ◽  
Learning Needs ◽  
Democratic Leadership ◽  
Entrepreneurial Spirit ◽  
To Receive

Lesson study is a systematic, collaborative, and sustainable method of improving the quality of learning. Lesson study emphasizes the exploration of students’ learning needs; teacher openness towards learning difficulties encountered; the willingness of teachers to receive and provide advice and solutions to the difficulties encountered; and the consistency of the various parties to follow up the suggestions and solutions. Implementation of lesson study involving teachers, principals, and experts in the field of education. Kurikulum tingkat satuan pendidikan or abbreviated KTSP is operational curriculum formulated and implemented by each educational unit. KTSP has the characteristics, namely: giving broad autonomy to the educational unit, involving the community and parent participation, involving the democratic leadership of the principal, and require the support of a working team that is synergistic and transparent. KTSP based on the learning process, needs to be supported by a conducive learning environment and fun to be created by teachers.Teachers and principals in a professional, systematic and collaborative create an atmosphere that fosters independence, tenacity, entrepreneurial spirit, adaptive and proactive nature of the learning process. Thus, the learning needs of students who fulfilled optimally and professional ability of teacher who have increased on an ongoing basis, may usher in success – based learning KTSP. It means that the lesson study provides positive implications for the KTSP – based learning.

DAYA SAING EKSPOR PRODUK CPO INDONESIA DAN POTENSI HILIRISASI DIOLAH MENJADI BIODIESEL

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Danar Agus Susanto
Keyword(s):  
Palm Oil ◽  
Comparative Advantage ◽  
Global Market ◽  
National Standard ◽  
Revealed Comparative Advantage ◽  
Crude Palm Oil ◽  
A Value ◽  
World Demand ◽  
Domestic Use

ABSTRACTIndonesia is the world's largest producer of crude palm oil (CPO). Even so, the trend of international trade in Indonesian CPO products has decreased in the last 10 years with a value of -10.19%. The decline in world demand for CPO can be dealt with by utilizing CPO as a biofuel for domestic use, like biodiesel. The purpose of this study is to determine the competitiveness of Indonesian CPO in global trade and determine the parameters of the quality requirements of CPO and biodiesel. The competitiveness analysis method uses the Revealed Comparative Advantage (RCA) method, while the analysis of the potential quality of CPO as a biofuel is carried out by analyzing the Indonesian National Standard (SNI). CPO trade in the global market is controlled by Indonesia with a market share of 50% and Malaysia 25.6%, with the declining trade trend in the last 10 years relatively (2010-2019) of -6.91%. The level of competitiveness of Indonesian CPO products in 2019 is very strong, but on average in the last 10 years, the level of competitiveness of Indonesian CPO is no better than that of Malaysia. Indonesia has SNI 01-2901-2006 and SNI 7182: 2015 as a determinant of the quality of CPO and biodiesel products developed in Indonesia so that the CPO and biodiesel produced fullfil sacurity and safety aspects when used by consumers.Keywords: crude palm oil (CPO), competitivenessv, quality, Indonesian National Standard (SNI) ABSTRAKIndonesia merupakan negara produsen minyak kelapa sawit mentah/ Crude Palm Oil (CPO) terbesar di dunia. Meskipun demikian, tren perdagangan internasional produk CPO Indonesia mengalami penurunan dalam sepuluh tahun terakhir dengan nilai -10,19%. Penurunan permintaan dunia terhadap CPO, dapat disiasati dengan memanfaatkan CPO sebagai bahan bakar nabati untuk keperluan dalam negeri sebagai biodiesel. Tujuan penelitian ini adalah mengetahui daya saing CPO Indonesia dalam perdagangan global dan mengetahui parameter persyaratan mutu CPO dan biodiesel. Metode analisis daya saing menggunakan metode Revealed Comparative Advantage (RCA), sedangkan analisis potensi kualitas CPO sebagai biofuel dilakukan dengan menganalisis Standar Nasional Indoensia (SNI). Perdagangan CPO dalam pasar global dikuasai oleh Indonesia dengan pangsa pasar 50% dan Malaysia 25,6%, dengan tren perdagangan relatif menurun dalam 10 tahun terakhir (2010-2019) sebesar -6,91%. Tingkat daya saing produk CPO Indonesia pada tahun 2019 sangat kuat, namun secara rata-rata dalam 10 tahun terakhir, tingkat daya saing CPO Indonesia tidak lebih baik dari Malaysia. Indonesia memiliki SNI 01-2901-2006 dan SNI 7182:2015 sebagai penentu kualitas produk CPO dan biodiesel yang dikembangkan di Indonesia, sehingga CPO dan biodiesel yang dihasilkan memenuhi aspek keamanan dan keselamatan ketika digunakan oleh konsumen.Kata kunci: crude palm oil (CPO), daya saing, kualitas, Standar Nasional Indonesia (SNI)

Use of SAMe-TT₂R₂ Score to Predict the Quality of Anticoagulation Control in Patients with Atrial Fibrillation Receiving Warfarin in Thailand

2020 ◽  
Vol 103 (6) ◽  
pp. 548-552
Keyword(s):  
Atrial Fibrillation ◽  
Therapeutic Range ◽  
Statistical Significance ◽  
Discrimination Performance ◽  
Poor Quality ◽  
Chi Square ◽  
Exact Test ◽  
Time In Therapeutic Range ◽  
Anticoagulation Control

Objective: To predict the quality of anticoagulation control in patients with atrial fibrillation (AF) receiving warfarin in Thailand. Materials and Methods: The present study retrospectively recruited Thai AF patients receiving warfarin for three months or longer between June 2012 and December 2017 in Central Chest Institute of Thailand. The patients were classified into those with SAMe-TT₂R₂ of 2 or less, and 3 or more. The Chi-square test or Fisher’s exact test was used to compare the proportion of the patients with poor time in therapeutic range (TTR) between the two groups of SAMe-TT₂R₂ score. The discrimination performance of SAMe-TT₂R₂ score was demonstrated with c-statistics. Results: Ninety AF patients were enrolled. An average age was 69.89±10.04 years. Most patients were persistent AF. An average CHA₂DS₂-VASc, SAMe-TT₂R₂, and HAS-BLED score were 3.68±1.51, 3.26±0.88, and 1.98±0.85, respectively. The present study showed the increased proportion of AF patients with poor TTR with higher SAMe-TT₂R₂ score. The AF patients with SAMe-TT₂R₂ score of 3 or more had a larger proportion of patients with poor TTR than those with SAMe-TT₂R₂ score of 2 or less with statistical significance when TTR was below 70% (p=0.03) and 65% (p=0.04), respectively. The discrimination performance of SAMe-TT₂R₂ score was demonstrated with c-statistics of 0.60, 0.59, and 0.55 when TTR was below 70%, 65% and 60%, respectively. Conclusion: Thai AF patients receiving warfarin had a larger proportion of patients with poor TTR when the SAMe-TT₂R₂ score was higher. The score of 3 or more could predict poor quality of anticoagulation control in those patients. Keywords: Time in therapeutic range, Poor quality of anticoagulation control, Warfarin, SAMe-TT₂R₂, Labile INR

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