scholarly journals Oxidative carbonilation of liver tissue proteins under the influence of pesticide based on glyphosate in a subchronic experiment

2020 ◽  
Vol 64 (6) ◽  
pp. 351-357
Author(s):  
Darya I. Miroshnikova ◽  
Valerii N. Rakitskii ◽  
Maria A. Fomina ◽  
Valerii A. Kiryushin ◽  
Tatiana V. Motalova
Keyword(s):  
Oxidative Damage ◽  
Liver Tissue ◽  
Study Groups ◽  
The Body ◽  
Subcellular Fractions ◽  
Carbonyl Stress ◽  
Oxidative Carbonylation ◽  
Control Group ◽  
Tissue Homogenates ◽  
Oxidative Damage To Proteins

Introduction. Currently, the pathogenetic mechanisms of the action on the body of widely used glyphosate-based herbicides and the search for the markers of the health status that changes under the influence of these herbicides remain open for study and discussion. Carbonyl derivatives of proteins can be used as the markers of oxidative stress caused by the herbicidal composition of the isopropylamine salt of glyphosate. The purpose of this study was to assess the severity of oxidative carbonylation of liver tissue proteins under the influence of a glyphosate-based pesticide in a subchronic experiment. Material and methods. The material for the study was subcellular fractions of liver tissue homogenates obtained from 90 Wistar rats, which were administered solutions of glyphosate isopropylamine salt at doses of 280 mg/kg and 100 mg/kg per os for 3 months. The severity of oxidative carbonylation of proteins was determined by the method of R.L. Levine modified by E.E. Dubinina. Results. Statistically significant changes in the content of products of oxidative damage to proteins at various stages of the experiment in the study groups were obtained compared with the control group due to a significant increase in the products of oxidative damage to proteins in subcellular fractions of liver tissue. Conclusion. The changes in carbonyl stress indices and the decrease in the reserve-adaptive potential indicate the depletion of antioxidant protection in the liver cells. A statistically significant increase in secondary markers of carbonyl stress after 1 month from the start of seeding of animals compared with the control with a slight increase in this index after 3 months may indicate the launch of adaptation mechanisms, including the induction of proteolytic utilization of oxidized proteins or additional protein synthesis.

scholarly journals A randomised, open label comparative study of hydroxychloroquine with betamethasone oral mini pulse in the management of patients with alopecia areata

2021 ◽  
Vol 10 (2) ◽  
pp. 187
Author(s):  
Jeyasudha Jambusayee ◽  
Kulur Mukhyaprana Sudha
Keyword(s):  
Alopecia Areata ◽  
Life Quality ◽  
Autoimmune Disorder ◽  
Study Groups ◽  
Pulse Therapy ◽  
The Body ◽  
Control Group ◽  
Study Group ◽  
Open Label ◽  
Significant Difference

Background: Alopecia areata is an autoimmune disorder causing patchy hair loss on scalp and other parts of the body and leading to poor self-esteem and anxiety in patients. Treatment with topical or systemic drugs like steroids or other immunosuppressants is associated with adverse effects. Hydroxychloroquine is an antimalarial drug, with T cell modulating function. This study was undertaken to assess the safety, efficacy and tolerability of Hydroxychloroquine in Alopecia areata compared to betamethasone oral mini pulse (OMP) therapy. Methods: 60 patients with alopecia areata were randomized into two groups of 30 each. Control group received tab. betamethasone 5 mg/day on two consecutive days of week for 12 weeks and Study group received tab. hydroxychloroquine 200 mg/day for 12 weeks. They were followed-up for further 12 weeks. Scale of alopecia tool, dermatology life quality index and global assessment at baseline, 12 weeks and 24 weeks were used to assess the outcome.Results: 94 patients were screened and 60 patients were included. All patients completed the study. At the end of 12 weeks, there was a statistically significant reduction in SALT and DLQI scores in both control and study groups. But at the end of 24 weeks, the study group showed an increase in the scores. Relapses were more in the study group. No significant difference in the incidence of adverse events was noted between the two groups.Conclusions: Hydroxychloroquine 200 mg/day is less efficacious in the management of alopecia areata in comparison to betamethasone oral mini pulse therapy.

scholarly journals Antioxidant Barrier, Redox Status, and Oxidative Damage to Biomolecules in Patients with Colorectal Cancer. Can Malondialdehyde and Catalase Be Markers of Colorectal Cancer Advancement?

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 637 ◽  
Author(s):  
Zińczuk ◽  
Maciejczyk ◽  
Zaręba ◽  
Romaniuk ◽  
Markowski ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Oxidative Damage ◽  
Redox Status ◽  
Control Group ◽  
Tumour Invasion ◽  
Reducing Ability ◽  
Glycation End Products ◽  
Total Antioxidant ◽  
Oxidative Damage To Proteins ◽  
Oxidant Status

This study is the first to assess the diagnostic utility of redox biomarkers in patients with colorectal cancer (CRC). Antioxidant barrier (Cu,Zn-superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), uric acid (UA), reduced glutathione (GSH)), redox status (total antioxidant (TAC)/oxidant status (TOS), ferric reducing ability (FRAP)), and oxidative damage products (advanced glycation end products (AGE), advanced oxidation protein products (AOPP), malondialdehyde (MDA)) were measured in serum/plasma samples of 50 CRC patients. The activity of SOD was significantly higher whereas the activity of CAT, GPx and GR was considerably lower in CRC patients compared to the control group (p < 0.0001). Levels of UA, TOS, and OSI and concentrations of AGE, AOPP, and MDA were significantly higher, and the levels of GSH, TAC, and FRAP were considerably lower in CRC patients compared to the healthy controls (p < 0.0001). AUC for CAT with respect to presence of lymph node metastasis was 0.7450 (p = 0.0036), whereas AUC for MDA according to the depth of tumour invasion was 0.7457 (p = 0.0118). CRC is associated with enzymatic/non-enzymatic redox imbalance as well as increased oxidative damage to proteins and lipids. Redox biomarkers can be potential diagnostic indicators of CRC advancement.

scholarly journals PENGARUH PENGGUNAAN KURSI PERSALINAN BC-MK15 TERHADAP KETIDAKNYAMANAN BIDAN DALAM PERTOLONGAN PERSALINAN

2020 ◽  
Vol 7 (1) ◽  
pp. 39-54
Author(s):  
Hani Sutianingsih ◽  
Johanes C Mose ◽  
Farid Husin
Keyword(s):  
Musculoskeletal Disorders ◽  
Intervention Group ◽  
Simple Random Sampling ◽  
Body Part ◽  
Study Groups ◽  
The Body ◽  
Control Group ◽  
Delivery Care ◽  
Significance Level ◽  
And Control

Midwife has risks in their work, such as at first stage delivery care, they have risk of unnatural work posture, while in the second and third stage midwife experiences a body irregularity which can be seen in the spinal segment and postural instability. Unusual work postures continuously can cause musculoskeletal disorders (MSD). The etiology of musculoskeletal disorders is very complicated and controversial, so the lack of information can lead to more difficult risk management, but by detecting discomfort is very useful as an indicator of the risk of musculoskeletal disorders, thus minimizing the discomfort may contribute to reducing risk of musculoskeletal disorders, since both are known associated with exposure to the musculoskeletal system by biomechanical loads. The importance of the ergonomics aspect for the midwife to reduce the discomfort of the body in doing it’s work, is strongly supported by the appropriate facilities such as the BC-MK15 birth chair. This research is quasi experimental with simple random sampling, subject is divided into two groups, intervention and control (22 midwives each group) who work at Puskesmas Garuda, Puter and Ibrahim Adji Bandung. Discomfort scoring using Body Part Discomfort Scale (BPDS). Statistical test using Kolmogorov -Smirnov analysis to see the difference of discomfort between treatment and control group for each stage of delivery care, and Chi-Square test to see the effect between the two study groups also Mann-Whitney test  to see the discomfort diffrences between each group for the whole stage of delivery care (stage I-III). The significance level been decided for p <0.05 . The results showed that there was an effect of the use of the BC-MK15 birth chair to the midwife discomfort level  for each body part in every stage during delivery care, and there was a lower median rate of discomfort in the intervention group compare to the control group The conclusion was the use of BC-MK15 birth chairs can decrease midwife discomfort for every stage during delivery care in the intervention group compare to the control group.

EFFECTIVENESS OF NORDIC WALKING AND PHYSICAL TRAINING IN IMPROVING BALANCE AND BODY COMPOSITION OF PERSONS WITH DOWN SYNDROME

2018 ◽  
Vol 28 (82) ◽  
pp. 11-19
Author(s):  
Amadeusz Skiba ◽  
Agnieszka Stopa ◽  
Iwona Sulowska ◽  
Wiesław Chwała ◽  
Anna Marchewka
Keyword(s):  
Body Composition ◽  
Down Syndrome ◽  
Physical Training ◽  
Training Group ◽  
Study Groups ◽  
Positive Influence ◽  
The Body ◽  
Control Group ◽  
Clinical Test ◽  
Nordic Walking

Aim The aim of this study was to evaluate the effects of Nordic Walking training and physical training on the balance and body composition in adult people with Down syndrome. Basic procedures We enrolled 32 subjects with Down syndrome, aged 25-40 years with moderate intellectual disability. They were randomly divided into three groups: Nordic Walking training group, physical training group and control group with no intervention. Training sessions were held for 10 weeks at a frequency of 3 times a week. Subjects were examined twice: 1 week before training and a week immediately after intervention. To evaluate balance we applied modified Clinical Test for Sensory Integration and Balance (mCTSIB) on BioSway platform. Evaluation of body composition was assessed with Tanita Body Composition Analyzer TBF – 300. Results After training, in the mCTSIB statistically significant changes were observed only in the Nordic Walking group. In the control group in the mCTSIB the changes were not reported. There were observed improvements in the body composition after Nordic Walking training and in the control group there were deteriorated. Conclusions The research shows that regular physical activity such as Nordic Walking training has positive influence on the balance in people with Down syndrome. The changes were greater in people participated in Nordic Walking training rather than physical training. Both of the study groups presented improvement compared to controls. Key words: Down syndrome, Nordic Walking, disability, balance, stability, BMI, mCTSIB, body composition, rehabilitation

scholarly journals A Study to Evaluate Use of Platelet Indices in Hyperdestructive Thrombocytopenia: A Two-year Experience from Tertiary Care Rural Hospital

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Roniya Francis ◽  
Shruthi N Shetageri ◽  
Roopa A N ◽  
S R Raja Parthiban
Keyword(s):  
Tertiary Care ◽  
Study Groups ◽  
The Body ◽  
Parasitic Infections ◽  
Control Group ◽  
Platelet Destruction ◽  
Distribution Width ◽  
Platelet Indices ◽  
Group I ◽  
Group Ii

Background: Thrombocytopenia is one of the most common causes of abnormal bleeding and is defined as platelet counts < 1.5 lakhs/cumm. Three processes can cause thrombocytopenia, namely: Deficient platelet production, accelerated platelet destruction, and abnormal pooling of the platelets within the body. Of these, accelerated platelet destruction is the most common cause for thrombocytopenia and has variety of etiologies. The usefulness of bone marrow analysis in assessing accelerated platelet destruction is still debated. Therefore, a new simple and non-invasive diagnostic approach for thrombocytopenia is needed. Aims and Objectives: The present study was done with an aim to evaluate the use of platelet indices, namely, mean platelet volume (MPV), Platelet Distribution Width (PDW), and Platelet Large Cell Ratio (P-LCR) in differentiating the various causes of hyperdestructive thrombocytopenia. Materials and Methods: This was a prospective study conducted over a period of 2 years and consisted of 206 cases of hyperdestructive thrombocytopenia. After recording relevant clinical details, platelet count along with platelet indices – MPV, PDW, and P-LCR was recorded. Based on the etiopathology identified, cases were categorized into three groups: Group I: Immunologic – cases of Immune thrombocytopenic purpura (ITP), Group II: Non-immune: Cases of sepsis and other non-immune causes of platelet destruction, and Group III: Viral and parasitic infections. Platelet indices were compared between the study groups and the control group which included 100 healthy individuals. Comparison was done among the three study groups as well. Results & Conclusions: Dengue accounted for the highest number of 131 (89.72%) cases in the study. MPV, PDW, and P-LCR were significantly higher (P < 0.0001) when compared to the healthy controls except P-LCR in Group II. A statistically significant increase in MPV was noted among ITP cases when compared to other causes of thrombocytopenia. There wa

scholarly journals Pathologic Changes in the Liver During Acute Exposure to Clozapine and Its Combination with Ethanol (Experimental Study)

2019 ◽  
Vol 15 (2) ◽  
pp. 27-35 ◽  
Author(s):  
O. L. Romanova ◽  
D. V. Sudukov ◽  
M. A. Golubev ◽  
M. L. Blagonravov ◽  
V. V. Goshkoev ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Blood Serum ◽  
Liver Tissue ◽  
Cellular Response ◽  
The Body ◽  
Venous Congestion ◽  
Acute Exposure ◽  
Male Rats ◽  
Control Group ◽  
Pathological Changes

Purpose: identification of pathological changes in the liver at early stages of the effect of clozapine and clozapine combined with ethanol to improve the diagnosis of this acute exposure.Materials and methods. The experiments were carried out on outbred male rats (n=15). The animals were split into 3 groups, 5 animals in each: 1) control (intact rats); 2) clozapine, 3) clozapine and ethanol. Clozapine at a dose of 150 mg/kg of the animal’s body weight was administered orally under anesthesia (chloralose), clozapine (150 mg/kg) with ethanol at a dose of 5 ml/kg — orally. Rats were removed from the experiment after 3 hours by decapitation. Samples of liver tissue were studied using light microscopy. During histological examination, the following morphological signs were evaluated: blood circulation disorder (presence of venous, capillary, and arterial congestion, appearance of venous hemorrhage), absence of nuclei staining, cytoplasm lumpiness, cytoplasm vacuolation, presence of necrosis foci, appearance of cellular response (a large quantity of leukocytes per power field). The assessment was done using the F-test. Forensic chemical analysis was carried out using a high-performance liquid chromatograph equipped with a mass detector. The content of clozapine, norclozapine, and clozapine-N-oxide in blood serum and liver homogenate of rats was evaluated.Results. In the control group, no pathological changes in liver tissue were observed. 3 hours after clozapine administration, venous congestion was detected. 3 hours after administration of clozapine and ethanol combination, venous congestion and absence hepatocyte nuclei staining were observed. It was found that after combined administration of clozapine and ethanol, the blood serum clozapine was 1.94-fold and clozapine-N-oxide was 2.3-fold lower than when clozapine was administered as a single drug while the content of norclozapine showed no difference.Conclusion. Changes detected in the liver, together with changes in other organs and findings of the forensic chemical analysis, allow establishing the fact of clozapine poisoning. Understanding of the processes going on in the body during such intoxications helps rendering medical assistance to victims more effectively.

scholarly journals The influence of silicium compounds on the content of calcium, phosphorus and some lipides in the plasma blood

2019 ◽  
Vol 21 (95) ◽  
pp. 41-46 ◽  
Author(s):  
A. I. Ivanitskaya ◽  
Ya. V. Lesyk
Keyword(s):  
Body Weight ◽  
Organic Compound ◽  
Blood Plasma ◽  
Study Groups ◽  
Sodium Metasilicate ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Control Group ◽  
Total Calcium ◽  
Preparatory Period

The aim of the study was to investigate the effects of nanotechnology and sodium metasilicate on the content of total calcium and inorganic phosphorus and individual lipids in the blood of rabbits from 52 to 110 days of life. Studies were conducted on young rabbits of Hyla breed in the private sector. Rabbits for the study were selected at the age of 41 days on the principle of analogues, weighing 1.2–1.4 kg, were divided into six groups (control and five experimental), 6 animals (3 males and 3 females) in each. Animals were kept in with adjustable microclimate and illumination in mesh cages measuring 50×120×30 cm, in accordance with modern animal health standards. The control group rabbits were fed without restriction a balanced granular compound feed, with free access to water. Animals I, II, and III of the study groups were fed a control group diet and fed citrate silicon for 24 hours, with a corresponding rate of 25; 50 and 75 µg Si/kg body weight. Young animals of the IV and V experimental groups were fed wit diet of the control group and water was given sodium metasilicate (Na2SiO3H2O) in the amount of 2.5 and 5.0 mg Si/kg body weight, respectively. The experiment lasted 68 days, including the preparatory period of 10 days, the experimental period – 58 days. In the preparatory period – at 52 days and experimental at 83 and 110 days of life (31 and 58 days of drinking additives) in 4 animals (2 males and 2 females) from the group blood samples were taken for biochemical studies. Studies have found that the concentration of total calcium in the blood plasma of rabbits III and IV study groups was higher by 9.6 and 6.4% (P < 0.05) for 58 days of the study compared with the control, indicating the stimulating effect of the organic compound silicon to activate the processes of assimilation of this macronutrient in the body of young rabbits. Silicone citrate in the large test quantities and sodium metasilicate in the blood plasma of animals of III, IV and V experimental groups respectively increased the level of inorganic phosphorus by 35.7; 28.5 and 35.7% (P < 0.05) than controls at day 58 of the study. In the final study period, the ratio of Calcium to Phosphorus in the animals of the II – V experimental groups was in the range of 2.0–1.73: 1, indicating a more pronounced effect of silicon compounds on the metabolism of Phosphorus during a longer period of supplementation. The content of triacylglycerols in the blood plasma of rabbits II and III of the experimental groups was lower (P < 0.05) at 31 and 58 days of the study compared with the control. The results obtained may indicate the activation of the processes of metabolic accumulation of plastic components of cell membranes and energy needs of body tissues. The use of silica organic compound reduced the cholesterol content by 37% (P < 0.05) in the blood of rabbits of the III experimental group at 31 days of the study. Whereas at the final stage of the experiment in animals II; In the 3rd and 4th research groups, its level was lower by 43.4; 36.9 and 42.2% (P < 0.05) compared to the control, indicating greater use of cholesterol by the organism with silica organic compound.

Artemisinin induces hormonal imbalance and oxidative damage in the erythrocytes and uterus but not in the ovary of rats

2014 ◽  
Vol 34 (1) ◽  
pp. 83-92 ◽  
Author(s):  
EO Farombi ◽  
AO Abolaji ◽  
IA Adedara ◽  
I Maduako ◽  
I Omodanisi
Keyword(s):  
Oxidative Damage ◽  
Corn Oil ◽  
Reproductive Toxicity ◽  
Study Groups ◽  
Female Rats ◽  
Malarial Parasite ◽  
Control Group ◽  
Group I ◽  
Hormonal Imbalance ◽  
The Impact

Artemisinin is an antimalarial drug previously reported to induce neurotoxicity and embryotoxicity in animal models. This study investigated the erythrocytes and reproductive toxicity potentials of artemisinin in female rats. Animals were randomly divided into four study groups of eight rats each. The control group (group I) received corn oil, the vehicle, while groups II–IV were orally exposed to 7, 35 and 70 mg kg−1 day−1 of artemisinin, respectively, by gastric intubation for 7 consecutive days. Subsequently, we evaluated the impact of artemisinin on the endocrine environment and selected markers of oxidative damage and antioxidant status of the erythrocytes, ovary and uterus. Artemisinin significantly increased hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels and decreased catalase, glutathione peroxidase and superoxide dismutase activities in erythrocytes and uterus of rats compared with control group ( p < 0.05). However, artemisinin did not alter ovarian MDA, H2O2, glutathione levels and catalase activity, while ovarian and uterine histological assessment revealed absence of visible lesions. Moreover, artemisinin significantly decreased follicle-stimulating hormone and increased progesterone levels compared with control ( p < 0.05). Thus, these data suggest that in the absence of malarial parasite infection, artemisinin induced hormonal imbalance and oxidative damage in the erythrocytes and uterus but spared the ovary of rats.

HEPATOPROTECTIVE ROLE OF LOVASTATIN - PREGNANE X RECEPTOR AGONIST IN LITHOCHOLIC ACID INDUCED LIVER CHOLESTASIS DISEASED RATS

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Parminder Nain ◽  
Mohit Dhingra ◽  
Jaspreet Nain
Keyword(s):  
Antioxidant Enzymes ◽  
Liver Tissue ◽  
Liver Toxicity ◽  
Lithocholic Acid ◽  
Pregnane X Receptor ◽  
The Body ◽  
Control Group ◽  
Dependent Manner ◽  
Experimental Conditions ◽  
Albino Rat

Objective: Pregnane X receptor (PXR), member of nuclear receptor family, an integral component of the body defence mechanism against chemical insult are expressed in the liver, gastrointestinal system & lungs. Some studies have shown that the lovastatin is pregnane X receptor (PXR) activation effect. Methods: In the present study the hepatoprotective effect of lovastatin was investigated against lithocholic acid induced liver toxicity. Liver markers in serum and antioxidant enzymes in liver tissue were assessed by using standard procedures. Results: The level of liver marker (such as SGOT & SGPT) and bilirubin were increased significantly (p<0.05) and antioxidant enzyme (i.e. SOD, GSH and CAT) were significantly (p<0.05) decrease in lithocholic acid treated groups as compared to control group. Lovastatin at doses of 0.1, 0.2, 0.3 mg/kg showed significantly (p<0.05) decrease in the levels of liver marker (SGOT & SGPT) and bilirubin as compared to the positive control group in both pre & post treated models. The antioxidant enzymes such as Superoxide dismutase (SOD), Glutathione (GSH) and Catalase (CAT) content in liver tissue were significantly (p<0.05) increase after administration of lovastatin at dose dependent manner in both pre & post treated models. Conclusions: The results of the present study indicates that under the present experimental conditions, lovastatin showed hepatoprotective abilities against lithocholic acid induced hepatotoxicity in albino rat. Keywords: Hepatoprotection, lovastatin, Lithocholic Acid, Pregnane X Receptor

scholarly journals Salivary Oxidative Stress Increases with the Progression of Chronic Heart Failure

2020 ◽  
Vol 9 (3) ◽  
pp. 769 ◽  
Author(s):  
Anna Klimiuk ◽  
Anna Zalewska ◽  
Robert Sawicki ◽  
Małgorzata Knapp ◽  
Mateusz Maciejczyk
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
Oxidative Damage ◽  
Salivary Protein ◽  
Control Group ◽  
Saliva Secretion ◽  
Potential Biomarker ◽  
Central Plasma ◽  
Oxidative Damage To Proteins ◽  
And Gender

The aim of the study was to evaluate the rate of reactive oxygen species (ROS) production, antioxidant barrier, and oxidative damage in non-stimulated (NWS) and stimulated (SWS) saliva as well as plasma/erythrocytes of 50 patients with chronic heart failure (HF) divided into the two subgroups: NYHA II (33 patients) and NYHA III (17 patients). The activity of superoxide dismutase and catalase was statistically increased in NWS of HF patients as compared to healthy controls. The free radical formation, total oxidant status, level of uric acid, advanced glycation end products (AGE), advanced oxidation protein products and malondialdehyde was significantly elevated in NWS, SWS, and plasma of NYHA III patients as compared to NYHA II and controls. We were the first to demonstrate that with the progression of HF, disturbances of enzymatic and non-enzymatic antioxidant defense, and oxidative damage to proteins and lipids occur at both central (plasma/erythrocytes) and local (saliva) levels. In the study group, we also observed a decrease in saliva secretion, total salivary protein and salivary amylase activity compared to age- and gender-matched control group, which indicates secretory dysfunction of salivary glands in patients with HF. Salivary AGE may be a potential biomarker in differential diagnosis of HF.

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