FORMULASI DAN EVALUASI KRIM EKSTRAK BAWANG TIWAI (Eleutherine bulbosa)

2017 ◽  
Vol 3 (1) ◽  
pp. 72
Author(s):  
Husnul Warnida ◽  
Henny Nurhasnawati
Keyword(s):  
Antioxidant Activity ◽  
Adverse Effects ◽  
Microbial Growth ◽  
Ethanolic Extract ◽  
Native People ◽  
Scavenging Activity ◽  
The Sun ◽  
Dpph Method ◽  
Varied Concentration ◽  
Viscosity Stability

Exposure of the skin to ultraviolet (UV) radiation from the sun has various adverse effects, photoaging and skin cancer are of great concern. The ability of botanical antioxidants for prevention of photocarcinogenesis and photoaging had been widely known. Bawang tiwai (Eleutherine bubosa) is traditionally used by the native people of Kalimantan for cancer treatment. The ethanolic extract of bawang tiwai originated from Banjarbaru district, South Kalimantan had strong antioxidant activity. The objective of the study was to formulate Eleutherine bulbosa extract into cream and evaluate its scavenging activity. Antioxidant activity of Eleutherine bulbosa cream was assessed by 2-diphenyl-1-picrylhydrazyl (DPPH) method. The Eleutherine bulbosa extract was incorporated into five cream formulas in varied concentration of emulsifying wax and cetyl alcohol. Evaluation of cream including physical appearance, pH, viscosity, stability, and microbial growth test. The result showed that Eleutherine bulbosa cream has antioxidant activity and good physicochemical quality

scholarly journals Antioxidant activity of ethanolic extract of Penicillium chrysogenum and Penicillium fumiculosum

2014 ◽  
Vol 68 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Violeta Jakovljevic ◽  
Jasmina Milicevic ◽  
Jelica Stojanovic ◽  
Slavica Solujic ◽  
Miroslav Vrvic
Keyword(s):  
Antioxidant Activity ◽  
Penicillium Chrysogenum ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Chelating Ability

The aim of this study was to investigate the biological and chemical activity on two species of fungi of the genus Penicillium isolated from wastewater. On the selected species of fungi the different antioxidant activity assays were carried out: DPPH free-radical scavenging activity, total antioxidant activity, Fe2+- chelating ability and Fe3+- reducing power. Total phenol content was also determinate for ethanolic extract of mycelia. Penicillium chrysogenum ethanolic extract contained higher total phenolic content and better total antioxidant capacity as well as ferrous ion chelating ability. Penicillium fumiculosum ethanolic extract showed higher DPPH free-radical scavenging activity, as well as reducing power. Based on the obtained results it can be concluded that two types of fungi are potential new sources of natural antioxidants.

scholarly journals Evaluation antioxidant activity and corrosion inhibition of C38 in Hydrochloric acid medium by dried lemon peels of Kenitra Marrakech cities in Morocco and Taiz town in Yemen: A Comparative study

2021 ◽  
Vol 119 ◽  
pp. 04001
Author(s):  
Khaled Abdu ◽  
Rahma Erahioui ◽  
Khadija Khedid ◽  
Hefdhal deen ◽  
Maha Elhawary ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
Corrosion Inhibition ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Fatty Tissue ◽  
Scavenging Activity ◽  
Ic50 Values

This paper presents a comparative study about the evaluation of antioxidant activity and corrosion inhibition in Kenitra, Marrakesh and Taiz. An interesting topic, indeed polyphenols can improve or help to reduce oxidative stress in the treatment of digestive problems, weight management difficulties, diabetes, hypertension, arteriosclerosis and damage fatty tissue. Therefore, the authors deal with the measurement of polyphenol content and evaluation of the antioxidant activity of lemon peels in Kenitra , Marrakesh and Taiz.The authors performs empirical analyzes on lemon peels. The total polyphenol contents of the ethanolic extract of lemon peels were measured. It was to be 30. 23, 26. 346 and 20.961 mg/CE/g in Kenitra, Marrakech, and Taiz, respectively. Moreover, the DPPH radical scavenging activity of ethanolic extract of dried lemon peels was higher than 200μg/ml concentration. They were 73.47%, 47.36%, and 32.09in Kenitra, Marrakech, and Taiz, respectively. Also, the IC50 values of ethanolic extracts calculated from the percentage inhibitions at the same concentration. Inhibition (IC50) which obtained in Kenitra was 123.089 μg/ml. It was lower compared to the ethanol extract of Marrakech and Taiz that were 197.418, 276.750μg/mL, respectively. Therefore, the extract which is containing a high amount of phenolic is showed high radical scavenging activity. In addition, the maximum inhibition efficiencies for 2 mL L-1 of the inhibitor at 298 k were 98, 12% and 84, 85 % in Kenitra and Taiz, respectively. These values obtained through polarization curve measurements.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND GC-MS ANALYSIS OF THE ETHANOLIC EXTRACTS OF TERMINALIA BELLERICA ROXB (BAHEDA)

2016 ◽  
Vol 8 (11) ◽  
pp. 275
Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Neeraja Dwivedi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Terminalia Bellerica ◽  
Ms Analysis ◽  
Phytochemical Constituents

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.Methods: The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC50 for FRSA (62 µg/ml), SARSA (39 µg/ml) and HRSA (27 µg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC50 for LPO (48 µg/ml) whereas TBS showed lowest IC50 for FTC (109 µg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.

scholarly journals Antioxidant Activity Analysis and Standardization of Parkia speciosa (Petai) Pods Ethanol Extract

2019 ◽  
Vol 4 (1) ◽  
pp. 5 ◽  
Author(s):  
Najma Annuria Fithri ◽  
Fitrya Fitrya ◽  
Tia Shabrina ◽  
Diva Yulanri
Keyword(s):  
Antioxidant Activity ◽  
Water Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Structural Stabilization ◽  
Dpph Method ◽  
Research Outcome ◽  
Phenolic And Flavonoid

Parkia speciosa (petai) pods are rarely used and considered as waste despite their phenolic and flavonoid content. Phenolic and flavonoid content in plants are known to exhibit antioxidant activity. Antioxidants are known to potentially act agains radical and mediate the damage by structural stabilization. This research was conducted to analyze the antioxidant potential of petai pods using DPPH method. Analysis showed the IC50 of petai pods ethanolic extract was 75.72 ppm, which indicate strong acting antioxidant. Standardization of extracts were determined to manage the quality of extract, including the analysis of density, water content, ash values, and quantification of total phenolic and flavonoid content. Research outcome indicate the extract retain its quality during storage and use which confrmed to standardization requirements of water content and ash values. Total phenolics and flavonoid obtained were 272.45 mg/gGAE and 243.2029 mg/gQE respectively. Based from this research, petai pods ethanolic extract present strong antioxidant activity which was contributed by high existing content of flavonoid and phenolic compounds.

scholarly journals Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

scholarly journals Chemical Constituents Antioxidant and Anticholinesterasic Activity ofTabernaemontana catharinensis

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Carla Nicola ◽  
Mirian Salvador ◽  
Adriana Escalona Gower ◽  
Sidnei Moura ◽  
Sergio Echeverrigaray
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Neurodegenerative Diseases ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Alkaloid Content ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The present work aimed to analyze the alkaloid content of the ethanolic extract ofTabernaemontana catharinensis(Apocynaceae family) and its fractions as well as to evaluate their antioxidant and anticholinesterasic activities. The analyses of the ethanolic extract ofT. catharinensisby mass spectrometry allowed identifying the presence of the alkaloids 16-epi-affinine, coronaridine-hydroxyindolenine, voachalotine, voacristine-hydroxyindolenine, and 12-methoxy-n-methyl-voachalotine, as well as an alkaloid withm/z385.21 whose spectrum suggests a derivative of voacristine or voacangine. The extract and its alkaloid rich fractions showed antioxidant activity, especially those that contain the alkaloidm/z385.21 or 16-epi-affinine with DPPH scavenging activity (IC50) between 37.18 and 74.69 μg/mL. Moreover, the extract and its fractions exhibited anticholinesterasic activity, particularly the fractions characterized by the presence of 12-methoxy-n-methyl-voachalotine, with IC50= 2.1 to 2.5 μg/mL. Fractions with 16-epi-affinine combined good antioxidant (IC50= 65.59 to 74.69 μg/mL) and anticholinesterasic (IC50= 7.7 to 8.3 μg/mL) activities, representing an option for further studies aimed at treating neurodegenerative diseases.

scholarly journals PHYTOCHEMISTRY AND ANTIOXIDANT ACTIVITY OF SOURSOP (ANNONA MURICATA) LEAVES

2019 ◽  
pp. 1-6
Author(s):  
FONA QORINA ◽  
ADE ARSIANTI ◽  
QOTRUNNADA FITHROTUNNISA ◽  
NADZILA ANINDYA TEJAPUTRI
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Folk Medicine ◽  
Parasitic Infection ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Annona Muricata ◽  
Tropical Plant ◽  
Ic50 Value ◽  
Dpph Method

Objective: Soursop (Annona muricata) is a tropical plant which has been utilized as a folk medicine to treat many diseases including cancer, inflammation and parasitic infection. In this study, we investigated its phytochemistry properties and antioxidant activity against free radicals. Methods: Annona muricata leaves were extracted in three different solvents: ethanol, ethyl acetate and n-hexane. Afterwards, a phytochemistry test and the thin layer chromatography (TLC) method were used to evaluate bioactive compounds contained in the three different extracts. Antioxidant activity from the semi-polar (ethyl acetate) and polar (ethanol) solvents were evaluated by the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, and the antioxidant activity is expressed by IC50 value. The results were compared to ascorbic acid as a positive control. Results: The phytochemistry test showed that the extracts were positive for flavonoids, steroids, alkaloids, glycosides and tannins. Moreover, TLC analysis revealed that there were three chemical compounds contained in the extracts. The results from the DPPH method were that ethanolic extract was shown to have the most potent antioxidant activity with an IC50 value of 35.51 ppm. Conclusion: The ethanolic extract of Annona muricata could be developed as the next promising natural antioxidant source.

scholarly journals Antioxidant Activities of Phycocyanin: A Bioactive Compound from Spirulina platensis

2020 ◽  
pp. 73-85 ◽  
Author(s):  
Gamal A. Gabr ◽  
Salwa M. El-Sayed ◽  
Mohamed S. Hikal
Keyword(s):  
Antioxidant Activity ◽  
Spirulina Platensis ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Chemical Compositions ◽  
Scavenging Activity ◽  
Blue Green Algae ◽  
Wide Range

Background and Objectives: The cyanobacterium Spirulina also called blue green algae is a class of gram negative bacteria which possesses wide range of bioactive colored components as Phycocyanin, carotenoids and chlorophyll. Spirulina is one of the microalgae containing nutrients that have been used as a functional food in addition to therapeutic and pharmaceutical applications. This study aimed to evaluate the biochemical composition of Spirulina platensis biomass and its ethanolic and aqueous extracts, as well as, evaluate the antioxidant activities of the biomass, ethanolic, aqueous and the purified Phycocyanin. Materials and Methods: The chemical compositions of Spirulina platensis were determined, as well as the antioxidant activity of extracts, Phycocyanin, Phycocyanopeptide and Phycocyanobilin using (DPPH) radical-scavenging activity. Results: Results show that biomass has higher total proteins (49.72±0.508%), total carbohydrates (10.3±0.330%), moisture content (7.5±0.685%), lipids (7.2±0.105%) and Minerals (6.9±0.130%). In biomass, the total phenols (51.20±0.25 µg/mL) and flavonoids (97.73±1.858 µg/mL) were high compared to the ethanolic (49.48±0.130 and 69.07±1.814 µg/mL) and aqueous (15.27±0.639 and 4.67±0.611 µg/mL) extracts respectively. In the phenolic compounds, pyrogallol was identified as the major compound in biomass and aqueous extract (63.85 and 12.33%) respectively, E-vanillic acid in ethanolic (18.20 %), whereas, hespirdin (3.517 and1.639%) were major flavonoids found in aqueous and ethanolic extracts respectively. The DPPH scavenging activity was found higher in ethanolic extract compared to aqueous while in bioactive, the order of antioxidant activity was Phycocyanin > Phycocyanobilin > Phycocyanopeptide. Conclusions: The study data regarding to Spirulina nutritional value, makes Spirulina an excellent choice when formulating diets and combating malnutrition. Furthermore, it is a strong antioxidant and could be used as alternative treatments as anticancer, antidiabetic, and anti-inflammatory agent.

Sign in / Sign up

Export Citation Format

Share Document