scholarly journals The Antioxidant Activity Analysis of the Ethanolic Extract of Banana Peel (Musa paradisiaca forma typica) with DPPH Method

2018 ◽  
Author(s):  
Novia Ariani ◽  
Laela Hayu Nurani
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Activity Analysis ◽  
Banana Peel ◽  
Musa Paradisiaca ◽  
Dpph Method

FORMULASI DAN EVALUASI KRIM EKSTRAK BAWANG TIWAI (Eleutherine bulbosa)

2017 ◽  
Vol 3 (1) ◽  
pp. 72
Author(s):  
Husnul Warnida ◽  
Henny Nurhasnawati
Keyword(s):  
Antioxidant Activity ◽  
Adverse Effects ◽  
Microbial Growth ◽  
Ethanolic Extract ◽  
Native People ◽  
Scavenging Activity ◽  
The Sun ◽  
Dpph Method ◽  
Varied Concentration ◽  
Viscosity Stability

Exposure of the skin to ultraviolet (UV) radiation from the sun has various adverse effects, photoaging and skin cancer are of great concern. The ability of botanical antioxidants for prevention of photocarcinogenesis and photoaging had been widely known. Bawang tiwai (Eleutherine bubosa) is traditionally used by the native people of Kalimantan for cancer treatment. The ethanolic extract of bawang tiwai originated from Banjarbaru district, South Kalimantan had strong antioxidant activity. The objective of the study was to formulate Eleutherine bulbosa extract into cream and evaluate its scavenging activity. Antioxidant activity of Eleutherine bulbosa cream was assessed by 2-diphenyl-1-picrylhydrazyl (DPPH) method. The Eleutherine bulbosa extract was incorporated into five cream formulas in varied concentration of emulsifying wax and cetyl alcohol. Evaluation of cream including physical appearance, pH, viscosity, stability, and microbial growth test. The result showed that Eleutherine bulbosa cream has antioxidant activity and good physicochemical quality

scholarly journals FORMULATION AND ANTIOXIDANT ACTIVITY OF NANO GEL ETHANOL EXTRACT OF KEPOK BANANA PEEL (MUSA X PARADISIACA L.)

2021 ◽  
Vol 12 (1) ◽  
pp. 21-25
Author(s):  
Henni Rosaini ◽  
Auzal Halim ◽  
Ica Elpitha Sandi ◽  
Indra Makmur ◽  
Ridho Asra ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Side Effects ◽  
Stratum Corneum ◽  
Active Ingredient ◽  
Ethanol Extract ◽  
Banana Peel ◽  
Active Ingredients ◽  
Magnetic Stirrer ◽  
Dpph Method ◽  
Gel Preparation

Kepok banana peel (Musa x paradisiaca L.) contains flavonoids that act as antioxidants. The antioxidant activity of the ethanol extract of Kepok banana peel can be used as an active ingredient of nano gel. Nano-sized preparations can increase the efficiency of the delivery of active ingredients with lesser side effects and also decrease the stratum corneum to make it easier to penetrate. The objectives of this study were to determine whether the ethanol extract of Kepok banana peel (Musa x paradisiaca L.) can be formulated as nano gel and to measure its antioxidant activity. The method of nano gel preparation was stirring technique by using a magnetic stirrer and a sonicator. The antioxidant activity was measured using DPPH method. The results showed that the ethanol extract of kepok banana peel could be formulated as nano gel with size F0 = 161.9 nm; F1 = 171.3 nm; F2 = 165.6 nm; F3 = 163.9 nm which fulfilled the nano gel size requirement (20-200 nm) and the potential zeta value F0 = -43.4 mV; F1 = -43.7 mV; F2 = -46.9 mV; F3 = -47.0 mV fulfilled the requirements (smaller than -30 mV and greater than +30 mV). The antioxidant activity showed that the IC50 of ethanol extract of was stronger than IC50 of 1% nano gel (198.0279 µg/ml : 282.8933 µg/ml).

scholarly journals The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity

2020 ◽  
Vol 9 (1) ◽  
pp. 8-15
Author(s):  
Galuh Widiyarti ◽  
◽  
Andini Sundowo ◽  
Euis Filailla ◽  
Joddy Arya Laksmono ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Trace Amount ◽  
Extraction Process ◽  
Activity Analysis ◽  
Hydraulic Press ◽  
Screw Press ◽  
Ic50 Value ◽  
Dpph Method ◽  
Twin Screw ◽  
Water Contents

The extraction process from leaves and twigs of gambier (Uncaria gambier Roxb)plant was conducted mechanically by using traditional hydraulic press, conventional screw press, and modified twin-screw press. The leaves and twigs of gambier plant was obtained from traditional farmer in Limapuluh Kota, West Sumatera, Indonesia. The water, ash and catechin contents of the gambier extracts were analyzed based on SNI 01-3391-2000 using spectrophotometry and thermogravimetry method. Antioxidant activity analysis of the extracts was also performed by 1,1-diphenyl-2-picrylhidrazyl (DPPH) method and compared tovitamin C as a standard antioxidant. The analysis results showed that the extracts contain no ash. Meanwhile, the catechin and water contents of the extracts were approximately 50 and 13% thus the extracts were classified as quality gambier 2. Other than that, analysis result of catechin and epicatechin contents of the extracts using HPLC and compared to the reference materials showed that catechins contents of the extracts using traditional hydraulic press, conventionalscrew press, and modified twin-screw press give catechin content in about 94.296-95.030%. However, epicatechin was detected in a trace amount. The antioxidant activity of the extracts were 2.5 times stronger than reference. The IC50 value of 4.37-4.52 µg/mL and was categorized as active antioxidant.

scholarly journals Antioxidant Activity Analysis and Standardization of Parkia speciosa (Petai) Pods Ethanol Extract

2019 ◽  
Vol 4 (1) ◽  
pp. 5 ◽  
Author(s):  
Najma Annuria Fithri ◽  
Fitrya Fitrya ◽  
Tia Shabrina ◽  
Diva Yulanri
Keyword(s):  
Antioxidant Activity ◽  
Water Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Structural Stabilization ◽  
Dpph Method ◽  
Research Outcome ◽  
Phenolic And Flavonoid

Parkia speciosa (petai) pods are rarely used and considered as waste despite their phenolic and flavonoid content. Phenolic and flavonoid content in plants are known to exhibit antioxidant activity. Antioxidants are known to potentially act agains radical and mediate the damage by structural stabilization. This research was conducted to analyze the antioxidant potential of petai pods using DPPH method. Analysis showed the IC50 of petai pods ethanolic extract was 75.72 ppm, which indicate strong acting antioxidant. Standardization of extracts were determined to manage the quality of extract, including the analysis of density, water content, ash values, and quantification of total phenolic and flavonoid content. Research outcome indicate the extract retain its quality during storage and use which confrmed to standardization requirements of water content and ash values. Total phenolics and flavonoid obtained were 272.45 mg/gGAE and 243.2029 mg/gQE respectively. Based from this research, petai pods ethanolic extract present strong antioxidant activity which was contributed by high existing content of flavonoid and phenolic compounds.

scholarly journals Pembuatan Kopi dari Kulit Pisang Kepok (Musa paradisiaca Linn) dan Aktivitas Antioksidannya

2019 ◽  
Vol 3 (1) ◽  
pp. 94
Author(s):  
Allikha Bias Mentari ◽  
Siti Nur Kholisoh ◽  
Taufik Nor Hidayat ◽  
Umar Hafidz Asy'ari Hasbullah
Keyword(s):  
Antioxidant Activity ◽  
Water Content ◽  
Bulk Density ◽  
Study Design ◽  
Ash Content ◽  
Banana Peel ◽  
Phenol Content ◽  
Musa Paradisiaca ◽  
Randomized Design ◽  
Fruit Maturity

Processing coffee from kepok banana peel is an option in diversifying food preparations and providing non-caffeine coffee. This study aims to study the characteristics of banana peel coffee based on fruit maturity and oven duration. In addition, it also analyzes total phenol and its antioxidant activity. The study design used a factorial randomized design with the first factor of fruit maturity (unripe and ripe) and the second factor of oven length (5, 10, and 15 minutes). Banana skin coffee has a yield ranging from 8.6 to 11.6%. Bulk density ranges from 0.43-0.48g/ml. Water content ranges from 3.9-6.39%. Ash content ranges from 0.92-6.79%. The content of phenol ranges from 0.6 to 2.46 mg PE/g. The coffee phenol content of ripe banana peel is larger than unripe. The longer oven increases the phenol content. IC50 and EC50 decrease with increasing phenol. The antioxidant activity of ripe banana peel coffee is larger than unripe

scholarly journals Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

scholarly journals PHYTOCHEMISTRY AND ANTIOXIDANT ACTIVITY OF SOURSOP (ANNONA MURICATA) LEAVES

2019 ◽  
pp. 1-6
Author(s):  
FONA QORINA ◽  
ADE ARSIANTI ◽  
QOTRUNNADA FITHROTUNNISA ◽  
NADZILA ANINDYA TEJAPUTRI
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Folk Medicine ◽  
Parasitic Infection ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Annona Muricata ◽  
Tropical Plant ◽  
Ic50 Value ◽  
Dpph Method

Objective: Soursop (Annona muricata) is a tropical plant which has been utilized as a folk medicine to treat many diseases including cancer, inflammation and parasitic infection. In this study, we investigated its phytochemistry properties and antioxidant activity against free radicals. Methods: Annona muricata leaves were extracted in three different solvents: ethanol, ethyl acetate and n-hexane. Afterwards, a phytochemistry test and the thin layer chromatography (TLC) method were used to evaluate bioactive compounds contained in the three different extracts. Antioxidant activity from the semi-polar (ethyl acetate) and polar (ethanol) solvents were evaluated by the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, and the antioxidant activity is expressed by IC50 value. The results were compared to ascorbic acid as a positive control. Results: The phytochemistry test showed that the extracts were positive for flavonoids, steroids, alkaloids, glycosides and tannins. Moreover, TLC analysis revealed that there were three chemical compounds contained in the extracts. The results from the DPPH method were that ethanolic extract was shown to have the most potent antioxidant activity with an IC50 value of 35.51 ppm. Conclusion: The ethanolic extract of Annona muricata could be developed as the next promising natural antioxidant source.

scholarly journals Antioxidant activity from ethanol extract of red seaweed (Galaxaura rugosa)

2021 ◽  
Vol 11 (2) ◽  
pp. 79
Author(s):  
Bhayu Gita Bhernama ◽  
Witri Maulidy Ayu ◽  
Cut Nuzlia
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Red Seaweed ◽  
Oxidation Reactions ◽  
Ic50 Value ◽  
Dpph Method

Antioxidants are compounds that can delay, reduce, slow down or inhibit oxidation reactions from free radical reactions. Antioxidants donate electrons to unstable free radicals so that these free radicals can be neutralized to not interfere with the body's metabolic processes. Red seaweed Galaxaura rugosa has potential as an antioxidant. The study aimed to determine the antioxidant activity of the ethanol extract of red seaweed Galaxaura rugosa against DPPH free radicals based on the IC50 value. Phytochemical testing and determination of antioxidant activity were carried out using the DPPH method (2,2-diphenyl-1-picrylhydrazyl), which was carried out quantitatively using a UV-Vis spectrophotometer. The results of the phytochemical screening of red seaweed ethanol extract contained alkaloids, flavonoids, saponins, steroids, and polyphenols and the IC50 value of red seaweed ethanol extract was 4.59 ppm, while the positive control for Vitamin C was 6.64 ppm. It was concluded that the ethanolic extract of red seaweed Galaxaura rugosa in the South Aceh District had high antioxidant potential, as evidenced by the small IC50 value of <50 µg/mL.Keywords: Antioxidants, Galauxara rugosa, phytochemicals, DPPH  ABSTRAKAktivitas antioksidan dari ekstrak etanol rumput laut merah Galaxaura rugosa Antioksidan merupakan senyawa yang mampu menunda, memperkecil, memperlambat atau menghambat reaksi oksidasi dari reaksi radikal bebas. Antioksidan menyumbangkan elektron kepada radikal bebas yang tidak stabil sehingga radikal bebas ini dapat dinetralkan agar tidak mengganggu jalannya proses metabolisme tubuh. Rumput laut merah Galaxaura rugosa berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan ekstrak etanol rumput laut merah Galaxaura rugosa terhadap radikal bebas DPPH berdasarkan nilai IC50. Pada penelitian ini dilakukan pengujian fitokimia dan penentuan aktivitas antioksidan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil) yang dilakukan secara kuantitatif menggunakan spektrofotometer UV-Vis. Hasil dari skrining fitokimia ekstrak etanol rumput laut merah mengandung senyawa alkaloid, flavonoid, saponin, steroid dan polifenol serta nilai IC50 ekstrak etanol rumput laut merah sebesar 4,59 ppm sedangkan kontrol positif Vitamin C sebesar 6,64 ppm. Ekstrak etanol rumput laut merah Galaxaura rugosa di Kabupaten Aceh Selatan memiliki potensi antioksidan yang tinggi. dibuktikan dengan nilai IC50 yang yang kecil yaitu < 50 µg/mL.Kata kunci: Antioksidan, Galauxara rugosa, Fitokimia, DPPH

scholarly journals Uji Aktivitas Antioksidan Kombinasi Infusa Daun Sirih (Piper betle L), Ekstrak Etanolik Tanaman Bundung (Actinuscirpus grossus) dan Kulit Jeruk Nipis (Citrus aurantifolia)

2021 ◽  
Vol 7 (1) ◽  
pp. 115-122
Author(s):  
Kunti Nastiti ◽  
Noval Noval ◽  
Darini Kurniawati
Keyword(s):  
Antioxidant Activity ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Positive Control ◽  
Piper Betle ◽  
Citrus Aurantifolia ◽  
Water Solvent ◽  
Dpph Method ◽  
Antioxidant Effects

Piper betle L., the leaf, is the most often used for treatment and has been scientifically proven to have antibacterial activity. Actinuscirpus grossus plants are commonly found in Kalimantan and have antimicrobial properties. Citrus aurantifolia peel has antioxidant effects and nutritious chemical compounds in medicine. The purpose of this study was to determine the effect of antioxidant activity in a combination of Piper betle L., leaf infusion, ethanolic extract of Actinuscirpus grossus plant, and Citrus aurantifolia peel. The research method was experimental by making Piper betle L. leaves infused with water solvent. Simplisia powder of Actinuscirpus grossus plants and Citrus aurantifolia peel extracted by maceration with 95% ethanol solvent. The combination of the three plants was then tested for antioxidants by the DPPH method. Quercetin is used as a positive control. The results showed that the combination of the three plants had moderate antioxidant activity (IC50 128 ppm), but the antioxidant activity was smaller than quercetin (IC50 16.88 ppm). The combination of Piper betle L., leaf infusion, ethanolic extract of Actinuscirpus grossus plant, and Citrus aurantifolia peel has a moderate antioxidant category.

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