scholarly journals Identification and Efforts to Control Infection Odontoglossum ringspot virus (ORSV) on Orchid

Author(s):  
Mahfut Mahfut

Odontoglossum ringspot virus (ORSV) is a orchid virus which infection most widely reported and has spread widely in the world, including in Indonesia. Viral infections can degrade the quality of the orchid plant so it is economically detrimental. To study a disease infection, identification is carried out first to determine the most appropriate method of control. These identification techniques include variations in infection symptoms, host range testing, testing of viral titers, observation of particle morphology, genome detection, and analysis of DNA and amino acid sequences. Efforts to control disease can be done through preventive and eradicative actions chemically, biologically, and based on artificial intelligence.

2017 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Mahfut Mahfut ◽  
Budi Setiadi Daryono ◽  
Tri Joko ◽  
Susamto Somowiyarjo

ABSTRACTNatural orchids are one of the important ornamental plants that were cultivated in tropical countries, including Indonesia. Virus infections has been important limiting factor in orchids cultivation because it decreases the orchids quality. Odontoglossum ringspot virus (ORSV) is one of the most reported virus infecting orchids which spread widely in the world. During 2010–2014 surveys of viral infections were conducted in Indonesia. The orchids were found infected by virus, showed symptoms of mosaic, mottle, chlorotic, necrotic, streak, wilting leaf, and ringspot on leaf surface. Detection with serological test DAS-ELISA showed only 11 from 125 samples were infected by ORSV with total incidence of 8,8%. Nine leaf samples of Phalaenopsis sp. were infected, respectively. Thus, it was concluded that Phalaenopsis is orchids genus which is the most abundantly and susceptibly infected by ORSV. The results proved that ORSV have entered and spread widely by infected orchids in orchids landscape (nursery), semi-natural forests (botanical gardens), and natural forest (national park) throughout Indonesia. This is the first report of ORSV infecting natural tropical orchids in Indonesia.Keywords: DAS-ELISA, Indonesia, nature tropical orchids, ORSVINTISARIAnggrek alam merupakan salah satu kekayaan flora asli negara tropis, termasuk Indonesia. Keberadaan di habitat aslinya sudah sangat berkurang yang disebabkan kerusakan hutan dan adanya penyakit. Infeksi virus masih menjadi faktor pembatas terpenting dalam budidaya dan pengembangan potensi anggrek alam. Odontoglossum ringspot virus (ORSV) adalah salah satu jenis virus yang dilaporkan paling banyak menginfeksi anggrek serta memiliki penyebaran yang luas di dunia. Selama 2010-2014 telah dilakukan survei lapangan terhadap infeksi virus di Indonesia. Beberapa anggrek yang ditemukan terinfeksi oleh virus menunjukkan gejala berupa mosaik, belang, klorosis, nekrosis, streak, daun layu, dan bercak cincin pada permukaan daun. Deteksi dengan uji serologis DAS-ELISA menunjukkan bahwa 11 dari 125 sampel terinfeksi oleh ORSV dengan total kejadian 8,8%. Masing-masing sembilan dari total sampel daun terinfeksi merupakan Phalaenopsis sp. Hal ini menjelaskan bahwa Phalaenopsis adalah genus anggrek yang paling cocok dan rentan terhadap infeksi ORSV. Hasil penelitian survei kejadian infeksi Odontoglossum ringspot virus (ORSV) membuktikan bahwa virus ini telah masuk dan menyebar secara luas oleh anggrek-anggrek alam tropis yang terinfeksi di pertamanan anggrek (nurseri), hutan semi-alami (kebun raya), dan hutan alam (taman nasional) di seluruh wilayah Indonesia. Ini merupakan laporan pertama mengenai infeksi ORSV terhadap anggrek-anggrek alam tropis di Indonesia.Kata kunci: anggrek alam tropis, DAS-ELISA, Indonesia, ORSV


2021 ◽  
Vol 1 (1) ◽  
pp. 23-34
Author(s):  
Fania Nur Izzati ◽  
Mahfut Mahfut ◽  
Eti Ernawiati ◽  
Sri Wahyuningsih

Orchid is one of the largest groups of flowering plants that are in great demand by society. Besides, the various flowers with a long vase life, the price of orchids is also stable and affordable. Phalaenopsis and Dendrobium are the most popular types of orchids. To anticipate this, orchid cultivation needs to be improved. However, orchid cultivation is not always accompanied by the effort of disease prevention, particularly those caused by viruses. The virus that most infects orchids is the Odontoglossum ringspot virus (ORSV). Symptoms that appear on the leaves are chlorosis, mosaic with a pattern of lines, circles, and necrotic rings/rings. Control of viral infections can utilize organisms that are able to induce resistance, namely mycorrhizae. Mycorrhizae will penetrate into the root cortex tissue and provide nutrients for the orchid. Mycorrhizae that are quite often found are Rhizoctonia and are applied by induction to orchids. Induction was undertaken by planting orchids in Rhizoctonia inoculum until anatomically a peloton structure (solid coils) was formed on the orchid roots. The aim of this study was to study the response of orchid plants in the form of percentage incidence and intensity of the disease and the level of plant resistance to ORSV infection in Phalaenopsis amabilis and Dendrobium discolour which had been induced by Rhizoctonia. This research was conducted at the Laboratory of Botany, Biology, FMIPA, Universitas Lampung, using a factorial completely randomized design (CRD). Factor 1 was the type of orchid and factor 2 was mycorrhizal induction, virus inoculation, and a combination of both with 4 replications. The finding indicated that Phalaenopsis amabilis was more susceptible to ORSV infection than Dendrobium discolour based on a higher incidence and intensity of the disease. Mycorrhizal induction and virus inoculation (MAV) in Dendrobium discolour showed an increase of resistance response compared to Phalaenopsis amabilis.


2019 ◽  
Vol 1 (6) ◽  
pp. 53-55
Author(s):  
M. S. Turchina ◽  
M. V. Bukreeva ◽  
L. Yu. Korolyova ◽  
Zh. E. Annenkova ◽  
L. G. Polyakov

Currently, the problem of early rehabilitation of stroke patients is important, since in terms of the prevalence of cerebrovascular diseases and disability after suffering a stroke, Russia is one of the first places in the world. The complex of medical rehabilitation of such patients should provide for the early and most complete restoration of all body functions, patient education for lost skills, re-socialization of the patient and improvement of the quality of life. One of the factors contributing to a significant reduction in the quality of life after a stroke is the development of chronic constipation. The article reflects the modern methods of correction of chronic constipation in patients with limited mobility.


2013 ◽  
Vol 1 (1) ◽  
pp. 100
Author(s):  
Selçuk Yurtsever

It has been known that both in the world and in Turkey a continuous change has been experienced in the provision of health services in recent years. In this sense by adopting the customer(client) focused approach of either public or private sector hospitals; it has been seen that they are in the struggle for presenting a right, fast, trustuble, comfy service. The purpose of this research is to measure the satisfaction degree, expectations and perceptions of the patients in Karabük State Hospital through comparison. In this context, the patient satisfaction scale which has been developed as a result of literature review has been used and by this scale it has been tried to measure the satisfaction levels of the patients in terms of material and human factors which are the two main factors of the service that was presented. In the study, with the scales of Servqual and 0-100 Points together, in the part of the analysis MANOVA have been used. The expectations and the perceptions of the patient has been compared first by generally and then by separating to different groups according to the various criterias and in thisway it has been tried to be measured their satisfaction levels. According to the results that were obtained, although, the satisfaction levels of the patients who have taken service from Karabük State Hospital are high in terms of thedoctors and the nurses; it has been reached to the result that their satisfaction levels are low in terms of the materials that have been used at the presenting of the service and the management.


Author(s):  
Viсtor Ognevyuk

The article deals with the world rating of Ukrainian educational sphere according to The Global Competitiveness Report and UNESCO Science Report. It shows comparative indices of Ukraine in contrast to the other countries of these world ratings according to the “Quality of primary education”, “Penetration of primary education”, “Penetration of secondary education”, “Quality of secondary education”, “Quality of education in Sciences”, “Quality of school management”, “School access to the internet” and others. The article also defines strategic directions of reforming Ukrainian education system to improve its position in the world international ratings.


2020 ◽  
Author(s):  
Isra Revenia

When we compare with the quality of the best education in the world, Indonesia can catagorized as far behind. This can be seen from the achievments of students who become rejected measuring education quality in improving the qualitu of education, education supervision has very important role in developing education quality. Supervision can be interpreted as a coaching activity that has been planned to assist teachers and staff and other school staff in carrying out work effectively so that it gets good results. Supervision is a process that is applied to a job that has been carried out and even evaluates and corrects the work I to match what was determined from the start.


2019 ◽  
Author(s):  
Rika ramadani ◽  
Hade Afriansyah

Progress in information technology that is so fast is expected to improve the quality of education in Indonesia. In the world of information technology education can help and support the learning process. Especially now all the learning process activities can be done online. Progress in information technology must also be supported by quality human resources. In this case the teacher is very instrumental in the utilization of information technology in the world of education. Because the teacher is one of the education supervisors who will encourage the advancement of the quality of education in Indonesia. But in reality the quality of teachers in Indonesia is inadequate. There are still many teachers who cannot use information technology in learning especially for teachers who are senior or old. As teacher supervisors, they must improve the quality of their performance in using technology. To improve the ability of teachers to use technology, ongoing training is needed to use technology. The role of the head of the school as a supervisor is also needed, namely the principal is obliged to supervise, control, and approach the teacher in terms of the use of technology in the learning process.


Edupedia ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 45-53
Author(s):  
Ilzam Dhaifi

The world has been surprised by the emergence of a COVID 19 pandemic, was born in China, and widespread to various countries in the world. In Indonesia, the government issued several policies to break the COVID 19 pandemic chain, which also triggered some pro-cons in the midst of society. One of the policies government takes is the closure of learning access directly at school and moving the learning process from physical class to a virtual classroom or known as online learning. In the economic sector also affects the parents’ financial ability to provide sufficient funds to support the implementation of distance learning applied by the government. The implications of the distance education policy are of course the quality of learning, including the subjects of Islamic religious education, which is essentially aimed at planting knowledge, skills, and religious consciousness to form the character of the students. Online education must certainly be precise, in order to provide equal education services to all students, prepare teachers to master the technology, and seek the core learning of Islamic religious education can still be done well.


2018 ◽  
Vol 6 (1) ◽  
pp. 675-695
Author(s):  
FAJRI DWIYAMA

Abstract: Islamic education institutions in Indonesia face two challenges, namely; internal and external. Internally, Islamic education is faced with the classic problem of the low quality of human resources in education management. The causes include poor quality and education staff development and selectivity of education personnel. Furthermore externally, Islamic education institutions faced three major issues; globalization, democratization, and liberalization. The issue of globalization that is identical with market orientation and mechanism also affects the world of education. That way in education also includes awareness about how to attract and convince the community of the type and model of education. From here, the education world is ready or not ready to be involved in competition to gain public trust. To face these challenges Islamic Education institutions must maximally manage and empower all elements of management in the management of educational institutions. A good understanding of the elements of management can have an impact on the readiness of educational institutions to manage their institutions well in any condition to be the best and foremost. Keywords: Elements of Management, Management, Education, Islam


Author(s):  
Mohammad Karimi

Dental and oral health is an important part that plays a significant role in the quality of life of people in our society, especially children, but due to insufficient attention, tooth decay in the world is increasing every year. Promoting oral hygiene requires the people's easy access to primary oral health care and the use of these services should be classified.


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