scholarly journals Antioxidant capacity of plant raw material of Scutellaria baicalensis Georgi

10.5219/1090 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 614-621
Author(s):  
Olena Vergun ◽  
Liudmyla Svydenko ◽  
Olga Grygorieva ◽  
Oksana Shymanska ◽  
Dzhamal Rakhmetov ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Pharmacological Study ◽  
Reducing Power ◽  
Botanical Garden ◽  
Scutellaria Baicalensis ◽  
Raw Material ◽  
Strong Positive Correlation ◽  
Polyphenol Content ◽  
Scutellaria Baicalensis Georgi

The aim of this study was to evaluate antioxidant capacity of Scutellaria baicalensis Georgi from two regions of Ukraine: Kyiv city (M. M. Gryshko National Botanical Garden of NAS of Ukraine (NBG)) and Kherson region (Experimental Facility “Novokakhovska” of Rice Research Institute of Ukrainian Academy of Agrarian Sciences (EFN of RRI)). Observation of plants and biochemical analyses conducted with plants collected in the stage of flowering. In study investigated and compared above-ground part of plants and separated organs: inflorescences, stems, leaves. Measured morphometric parameters (height of plants, length, and width of leaves, length, and diameter of inflorescence, the diameter of the stem) showed that the most variable was the length of inflorescence (12.79%) for NBG sample and diameter of the stem (33.33%) for EFN of RRI sample. Ethanolic extracts were screened for the antioxidant capacity. As standards were used gallic acid for polyphenol content (GAE), quercetin for flavonoids (QE), caffeic acid for phenolic acids (CAE), Trolox for antioxidant capacity (TE). The total content of polyphenol compounds was 42.43 – 86.13 mg GAE.g-1 DW (dry weight) (NBG sample) and 28.06 – 96.76 mg GAE.g-1 DW (EFN of RRI sample). The content of flavonoids was 9.39 – 62.97 mg QE.g-1 DW (NBG sample) and 10.64 – 66.07 mg QE.g-1 DW (EFN of RRI sample). The concentration of phenolic acids was 2.60 – 16.13 mg CA.g-1 DW (NBG sample) and 12.02 – 30.12 CA.g-1 DW (EFN of RRI sample). Antioxidant activity of plant extracts was measured by DPPH assay and reducing power method. The first method indicated an antioxidant ability 8.24 – 8.56 mg TE.g-1 DW (NBG sample) and 7.63 – 8.83 mg TE.g-1 DW (EFN of RRI sample). Reducing power of extracts was 51.48 – 306.09 mg TE.g-1 DW (NBG sample) and 63.33 – 260.24 mg TE.g-1 DW (EFN of RRI sample). Very strong positive correlation identified between total polyphenol content, total flavonoid content and reducing power. Scutellaria baicalensis is a rich source of antioxidants and potential raw of further pharmacological study in Ukraine as well as in other regions for improving and enrichment of relevant production.

scholarly journals Parameters of antioxidant activity of Galega officinalis L. and Galega orientalis Lam. (Fabaceae Lindl.) plant raw material

10.5219/1271 ◽  
2020 ◽  
Vol 14 ◽  
pp. 125-134
Author(s):  
Olena Vergun ◽  
Oksana Shymanska ◽  
Dzhamal Rakhmetov ◽  
Olga Grygorieva ◽  
Eva Ivanišová ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Pharmacological Study ◽  
Reducing Power ◽  
Total Content ◽  
Raw Material ◽  
Galega Officinalis ◽  
Dpph Method ◽  
Galega Orientalis ◽  
Plant Raw Material

The plant raw material of Galega officinalis L. (goat’s rue) and Galega orientalis Lam. (fodder galega) investigated in this study. These species are known as fodder crops with high productivity of green mass and as medicine plants. The current study was aimed to evaluate an accumulation in dry raw of selected plants the total content of phenolic acids (TPA) and flavonoids (TFC) as compounds with antioxidant activity (AA) by spectrophotometric method. AA by DPPH-method and phosphomolybdenum method (reducing power (RP)) was measured. Study of ethanolic extracts of G. officinalis showed accumulation of TPA in different organs in range from 3.65 to 15.17 mg.g-1 caffeic acid equivalent (CAE) and TFC from 10.08 to 65.75 mg.g-1 quercetin equivalent (QE), AA by DPPH-method from 6.02 to 8.45 mg.g-1 Trolox equivalent (TE) and RP of extracts by phosphomolybdenum method from 86.56 to 288.15 mg TE.g-1. In extracts of G. orientalis was identified TPA from 3.52 to 18.52 mg CAE.g-1 and TFC from 6.09 to 46.72 mg QE.g-1, antioxidant activity by DPPH-method from 6.80 to 8.48 mg TE.g-1 and antioxidant capacity by phosphomolybdenum method from 52.52 to 188.51 mg TE.g-1. It was established that less concentration of studied compounds found in the stems for both species. It should be noted that the content of phenolic acids in the leaves was decreased and flavonoids in stems increased during vegetation for both species. Content of phenolic acids in the generative organs and flavonoids in the leaves decreased in raw of G. orientalis during vegetation. Pearson’s correlation analysis demonstrated very strong relations between TFC and AA by DPPH, TPA and RP, TFC and RP for G. officinalis extracts. Very strong correlation in the extracts of G. orientalis found between TFC and RP, TPA and RP. Obtained results can be used in the further biochemical and pharmacological study.

scholarly journals Antioxidant capacity, polyphenol content and iron bioavailability from algae (Ulva sp., Sargassum sp. and Porphyra sp.) in human subjects

2008 ◽  
Vol 101 (1) ◽  
pp. 79-85 ◽  
Author(s):  
Maria N. García-Casal ◽  
José Ramírez ◽  
Irene Leets ◽  
Ana C. Pereira ◽  
Maria F. Quiroga
Keyword(s):  
Antioxidant Capacity ◽  
Marine Algae ◽  
Human Subjects ◽  
Reducing Power ◽  
Iron Bioavailability ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Antioxidant Power ◽  
Fe Content ◽  
Dose Dependent

Marine algae are easily produced and are good sources of Fe. If this Fe is bioavailable, algae consumption could help to combat Fe deficiency and anaemia worldwide. The objective of the present study was to evaluate Fe bioavailability, polyphenol content and antioxidant capacity from three species of marine algae distributed worldwide. A total of eighty-three subjects received maize- or wheat-based meals containing marine algae (Ulva sp., Sargassum sp. and Porphyra sp.) in different proportions (2·5, 5·0 and 7·5 g) added to the water to prepare the dough. All meals administered contained radioactive Fe. Absorption was evaluated calculating radioactive Fe incorporation in subjects' blood. The three species of marine algae were analysed for polyphenol content and reducing power. Algae significantly increased Fe absorption in maize- or wheat-based meals, especially Sargassum sp., due to its high Fe content. Increases in absorption were dose-dependent and higher in wheat- than in maize-based meals. Total polyphenol content was 10·84, 18·43 and 80·39 gallic acid equivalents/g for Ulva sp., Porphyra sp. and Sargassum sp., respectively. The antioxidant capacity was also significantly higher in Sargassum sp. compared with the other two species analysed. Ulva sp., Sargassum sp. and Porphyra sp. are good sources of bioavailable Fe. Sargassum sp. resulted in the highest Fe intake due to its high Fe content, and a bread containing 7·5 g Sargassum sp. covers daily Fe needs. The high polyphenol content found in Sargassum sp. could be partly responsible for the antioxidant power reported here, and apparently did not affect Fe absorption.

scholarly journals Comparative analysis of the antioxidant capacity and secondary metabolites accumulation in the fruits of rowan (Sorbus aucuparia L.) and some closely related species

2021 ◽  
Vol 32 (1) ◽  
pp. 3-8
Author(s):  
Y. L. Lykholat ◽  
O. O. Didur ◽  
N. O. Khromykh ◽  
V. R. Davydov ◽  
Y. S. Borodai ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Botanical Garden ◽  
Total Phenolic ◽  
Strong Positive Correlation ◽  
Sorbus Aucuparia ◽  
Fruit Peel ◽  
Antioxidant Power ◽  
Interspecific Differences

The paper presents the results of studying fruits antioxidant properties of several species, previously combined into the genus Sorbus L. (Rosaceae family). Ripe fruits were collected in September 2020 from the plants of Cormus domestica (L.) Spach, Sorbus aucuparia L., Karpatiosorbus latifolia (Lam.) Sennikov & Kurtto and Torminalis glaberrima (Gand.) Sennikov & Kurtto from the collection of the Botanical Garden of the Oles Honchar Dnipro National University. Total phenolic content (TPC), total flavonoids content (TFC), ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) were determined both in the fruits peel and pulp. In the fruits of all species, higher values of the determined indicators were found in the fruit peel than in the pulp. Moderate and strong positive correlation was found between FRAP and TPC as well between FRAP and TFC both in peel and pulp of all studied species. At the same time, notable interspecific differences of the phytochemical traits were established, indirectly confirming the ambiguity of the taxonomy of the genus Sorbus L. According to the fruits antioxidant properties and secondary metabolites accumulation, species K. latifolia (broad-leaved whitebeam) and T. glaberrima (wild service-tree) are the most promising for cultivation in arid conditions of the central steppe of Ukraine.

scholarly journals Experimental Paper. Intrapopulation variability of flavonoid content in roots of Baikal skullcap (Scutellaria baicalensis Georgi)

2017 ◽  
Vol 63 (1) ◽  
pp. 20-31 ◽  
Author(s):  
Olga Kosakowska
Keyword(s):  
Total Content ◽  
Dry Mass ◽  
Scutellaria Baicalensis ◽  
Raw Material ◽  
Flavonoid Content ◽  
Oroxylin A ◽  
Pharmacological Activities ◽  
Scutellaria Baicalensis Georgi ◽  
Wide Range ◽  
Important Medicinal Plant

SummaryIntroduction: Baikal skullcap (Scutellaria baicalensis Georgi) is an important medicinal plant, indigenous to Asia. Due to a wide range of pharmacological activities, its roots has been used for ages in Traditional Chinese Medicine. Recently, the species has become an object of interest of Western medicine, as well. Objective: The aim of the study was to determine the variability of Baikal skullcap population originated from Mongolia and cultivated in Poland, in terms of content and composition of flavonoids in the roots. Methods: The objects of the study were 15 individual plants, selected within examined population and cloned in order to obtain a sufficient amount of raw material. The total content of flavonoids in roots was determined according to Polish Pharmacopeia 6th. The qualitative analysis of flavonoids was carried out using HPLC, Shimadzu chromatograph. Results: The dry mass of roots ranged from 25.88 to 56.14 g × plant-1. The total content of flavonoids (expressed as a quercetin equivalent) varied between 0.17 and 0.52% dry matter (DM). Nine compounds were detected within the group, with oroxylin A 7-Oglucuronide (346.90-1063.00 mg × 100 g-1DM) as a dominant, which differentiated investigated clones at the highest degree (CV=0.27). Baicalin (391.40-942.00 mg × 100 g-1DM), wogonoside (324.00-641.10 mg × 100 g-1DM) and hesperetine 7-O-glucoside (163.00-346.32 mg × 100 g-1DM) were also present in a considerable amounts. Clone 7 was distinguished by the highest content of all investigated compounds, except wogonin and oroxylin A 7-O-glucuronide. Conclusions: Results obtained in present study show a high variability within Baical skullcap investigated population in respect of flavonoid compounds detected in roots. Thus, the results may be used in future investigations concerning the selection and breeding of this species.

scholarly journals STUDY OF BAICALIN HYDROLYSIS KINETICS IN THE PROCESS OF ITS EXTRACTION FROM SCUTELLARIA BAICALENSIS GEORGI ROOTS

2019 ◽  
Vol 7 (3) ◽  
pp. 129-137
Author(s):  
N. N. Boyko ◽  
D. I. Pisarev ◽  
E. T. Zhilyakova ◽  
A. Yu. Maljutina ◽  
O. O. Novikov ◽  
...  
Keyword(s):  
High Performance ◽  
Water Solution ◽  
Scutellaria Baicalensis ◽  
Raw Material ◽  
Order Kinetic ◽  
Hydrolysis Kinetics ◽  
Scutellaria Baicalensis Georgi ◽  
Analytical Wavelength ◽  
Kinetics Of ◽  
Method Of Extraction

The aim of this study was to investigate the kinetics of baicalin hydrolysis in the process of its extraction from Scutellaria baicalensis Georgi roots.Materials and methods. For the studies, Scutellaria baicalensis Georgi roots with a particle range of 0.1–0.5 mm were used. The method of extraction was a simple maceration during a specified period of time, the ratio of plant raw material : extractant was 1:10 w/v at the temperature of 24±1°С. Baicalin and baicalein contents were analyzed by reverse phase high performance liquid chromatography (RP HPLC) at the analytical wavelength of 275 nm. The extractant was a water solution of ethanol 26, 43, 59, 72, 81, 97±1% v/v. The time of the extraction was from 1 to 24 hours.Results. The experimental points of dependency of baicalin concentration in the extract on the time of extraction for ethanol solutions with a concentration of 43 and 72% v/v are closely approximated by a linear equation in coordinates lnC=f(t). The value of determination coefficient is more than R²˃0,99. Half lifetime for baicalin has been calculated: for ethanol with the concentration of 43% v/v it is 4.3±0.7 hours, and for ethanol with the concentration of 72% v/v it is 42.3±1.8 hours.Conclusion. Baicalin hydrolysis kinetics in the process of its extraction from Scutellaria baicalensis Georgi roots with 43 and 72% v/v ethanol concentration. has been studied. It has been established that the process of baicalin hydrolysis is well described by the first order kinetic equation. The constants of baicalin hydrolysis during its extraction from Scutelaria baicalensis roots with ethanol having different concentrations have been calculated. Recommendations on technology optimization for baicalin or baicalein extraction from Scutellaria baicalensis Georgi roots have been given. 

scholarly journals Impact of Cold versus Hot Brewing on the Phenolic Profile and Antioxidant Capacity of Rooibos (Aspalathus linearis) Herbal Tea

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 499 ◽  
Author(s):  
Elisabetta Damiani ◽  
Patricia Carloni ◽  
Gabriele Rocchetti ◽  
Biancamaria Senizza ◽  
Luca Tiano ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Polyphenol Content ◽  
Herbal Tea ◽  
Potential Health ◽  
Phenolic Profile ◽  
Aspalathus Linearis ◽  
Antioxidant Capacities ◽  
The Impact

Consumption of rooibos (Aspalathus linearis) as herbal tea is growing in popularity worldwide and its health-promoting attributes are mainly ascribed to its phenolic composition, which may be affected by the brewing conditions used. An aspect so far overlooked is the impact of cold brewing vs regular brewing and microwave boiling on the (poly) phenolic profile and in vitro antioxidant capacity of infusions prepared from red (‘fermented’, oxidized) and green (‘unfermented’, unoxidized) rooibos, the purpose of the present study. By using an untargeted metabolomics-based approach (UHPLC-QTOF mass spectrometry), 187 phenolic compounds were putatively annotated in both rooibos types, with flavonoids, tyrosols, and phenolic acids the most represented type of phenolic classes. Multivariate statistics (OPLS-DA) highlighted the phenolic classes most affected by the brewing conditions. Similar antioxidant capacities (ORAC and ABTS assays) were observed between cold- and regular-brewed green rooibos and boiled-brewed red rooibos. However, boiling green and red rooibos delivered infusions with the highest antioxidant capacities and total polyphenol content. The polyphenol content strongly correlated with the in vitro antioxidant capacities, especially for flavonoids and phenolic acids. These results contribute to a better understanding of the impact of the preparation method on the potential health benefits of rooibos tea.

Antioxidant capacity, total phenolic, carotenoid, and vitamin C contents of five table grape varieties from Algeria and their correlations

OENO One ◽  
2014 ◽  
Vol 48 (2) ◽  
pp. 153 ◽  
Author(s):  
Farida Derradji-Benmeziane ◽  
Rachid Djamai ◽  
Yves Cadot
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Physicochemical Parameters ◽  
Reducing Power ◽  
Table Grape ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Strong Positive Correlation ◽  
Total Acidity ◽  
Grape Varieties

<p style="text-align: justify;"><strong>Aim</strong>: The aim of this work was the determination of some antioxidants (total polyphenols (TPP), total flavonoids (TF), carotenoids, vitamin C, the antioxidant capacity (AC) (reducing power) and other physicochemical parameters, such as pH, total soluble solids (°Brix), total acidity and density, in an attempt to understand how the evaluated characteristics vary with different grape varieties.</p><p style="text-align: justify;"><strong>Methods and results</strong>: Grape is currently known as a fruit that presents a very high antioxidant activity that can inhibit the harmful effects of free radicals. In this study, five Algerian table grape varieties were tested : Cardinal, Gros noir, Muscat noir, Muscat blanc and Victoria. TPP, TF, carotenoids and AC were determined by using colorimetric assays, and the physicochemical parameters (pH, °Brix, total acidity and density) were measured using classical methods. The darker varieties (Gros noir and Muscat noir) had the highest levels of polyphenols, flavonoids, carotenoids and vitamin C and possessed a good reducing power. A strong positive correlation was found, especially between TPP, TF and AC.</p><p style="text-align: justify;"><strong>Conclusion</strong>: This study indicated that some Algerian grape varieties exhibit high antioxidant phenolic and carotenoid content, which can potentially be used as natural source of antioxidant.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: This study on the AC, the TPP, carotenoid and vitamin C contents of five table grape varieties from Algeria and their correlations is very unique, as it is the first study on varieties grown in this country.</p>

scholarly journals Variability of the antioxidant properties of Berberis fruits depending on the plant species and conditions of habitat

2018 ◽  
Vol 9 (1) ◽  
pp. 56-61 ◽  
Author(s):  
N. O. Khromykh ◽  
Y. V. Lykholat ◽  
I. M. Kovalenko ◽  
A. M. Kabar ◽  
O. O. Didur ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Plant Species ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Total Content ◽  
Practical Significance ◽  
Strong Positive Correlation ◽  
Study Results ◽  
Phenolics Content ◽  
Fruit Plants

Plant fruits, leaves, stems, and other parts are of high nutritional value, and are the source of physiologically active compounds, which can contribute to the treatment of many diseases caused by oxidative stress. Enrichment of the species spectrum of the fruit plants expands the possibilities of their use in dietary nutrition and human treatment. Unfortunately, the introduction of the new fruit plant species in industrial gardens in the Steppe Dnieper is limited to a large extent by the unfavorable climate. In this regard, the assessment of the possibility of realizing the genetic potential of fruit plants from different geographical areas in the steppe climate acquires both scientific and practical significance. The study was conducted on the basis of the fruit plants collection collection of the Botanical Garden of the DNU including four introduced species and one natural species from the genus Berberis. The anomalous weather conditions during the growing season of 2017 (snowfall in April followed by a drought in June) were accompanied by an earlier stage appearance of leaves, flowering and fruit ripening of all introduced plants, especially the Asian species B. amurensis and B. koreana, compared to the native species B. vulgaris. In accordance with the results obtained, fresh weight of the ripe fruits of Berberis species decreased in the order of B. amurensis > B. vulgaris > B. canadensis > B. koreana > B. x declinata. The highest total phenolics content, determined in the isopropanolic fruit extracts by Folin – Ciocalteau assay, was found in the fresh ripe fruits of B. koreana (1362 ± 66 mg GAE/100 g WW), followed by B. x declinata and B. vulgaris fruits (91% and 77% of the B. koreana phenolics content respectively). The highest total flavonoids content determined using the aluminum chloride method was revealed in the fruits of B. koreana (210 ± 6 mg RE/100 g FW) exceeding the content in fruits of other Berberis species by 1.1–2.1 times, while the lowest value (103 ± 4 mg RE/100 g FW) was found in the fruits of B. amurensis. The total reducing power, determined by RP assay, varied in the range from 5.0 to 9.6 mg AAE/100 g DW, and the highest levels were found in the fruits of B. koreana and B. x declinata (respectively, 9.6 ± 0.6 and 8.6 ± 0.5 mg AE/100 g DW) exceeding the reducing capacity of other Berberis species by 1.7–1.9 times. In the fruits of genus Berberis species strong positive correlation was found between the total reducing power and the total content of phenols (r = 0.87), as well as between the reducing power and the total content of flavonoids (r = 0.84). High correlation coefficients confirm the significant contribution of the Berberis fruit phenolic compounds, including the flavonoids, to the antioxidant capacity. So, the study results showed that fruits of all examined Berberis species can be an easily accessible source of antioxidants, however, the antioxidant capacity of fruits decreased in order of B. koreana > B. x declinata > B. vulgaris > B. amurensis > B. canadensis.

scholarly journals Seasonal Evaluation of Phlorotannin-Enriched Extracts from Brown Macroalgae Fucus spiralis

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4287
Author(s):  
Belén Almeida ◽  
Sónia Barroso ◽  
Ana S. D. Ferreira ◽  
Pedro Adão ◽  
Susana Mendes ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Reducing Power ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Polyphenol Content ◽  
Brown Macroalgae ◽  
Antioxidant Power ◽  
Four Seasons ◽  
Ferric Reducing Antioxidant Power ◽  
Fucus Spiralis

Fucus spiralis that was collected in the four seasons was submitted to an extraction with ethanol:water (crude extracts Et80), followed by a liquid–liquid fractionation with organic solvents (fraction He from n-hexane; aqueous fractions AQ1, AQ2, AQ3 and AQ4; ethyl acetate fraction EA), with the aim of obtaining phlorotannin-enriched extracts. All the extracts (Et80, He, AQ1, AQ2, AQ3, AQ4 and EA) that were obtained for the F. spiralis of the four seasons were evaluated for their antioxidant capacity and total phenolic compounds. The summer extracts presented the highest contents in polyphenols (TPC), as well as the highest ferric reducing antioxidant power (FRAP), when compared to the samples from the other seasons. The reductive percentage of the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) compound was similar between the seasons. For all the seasons, the EA extract showed the highest polyphenol content (TPC), and the highest antioxidant capacity (highest ferric reducing power (FRAP) and lowest concentration needed to reduce 50% of the DPPH compound), which is in agreement with a phlorotannin-enriched fraction. This study revealed that the polyphenol content and antioxidant power of the F. spiralis extracts are influenced by the time of harvest, as well as by the solvents used for their extraction.

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