The fertilizing quality of the reproductive material during cryopreservation

2021 ◽  
Author(s):  
Ion Balan ◽  
◽  
Nicolae Rosca ◽  
Gheorghi Boronciuc ◽  
Vladimir Buzan ◽  
...  
Keyword(s):  
Reproductive Material

scholarly journals Contribution of Plant Litter in Fragments of Dry Tropical Forest in Paraíba

2018 ◽  
Vol 10 (12) ◽  
pp. 437 ◽  
Author(s):  
Adão Batista de Araújo ◽  
Patrícia Carneiro Souto ◽  
Jacob Silva Souto ◽  
Fabio Junho Alves da Silva ◽  
Francisco de Assis Pereira Leonardo ◽  
...  
Keyword(s):  
Climatic Variability ◽  
Organic Matter Content ◽  
Plant Litter ◽  
Matter Content ◽  
Litter Production ◽  
Rainy Period ◽  
Caatinga Vegetation ◽  
Reproductive Material ◽  
Metal Frame

Deposition of plant litter in the environment represents the entrance into the system, with reflection on soil organic matter content and environmental quality of the site. The objective of this study was to estimate the deposition, accumulation and decomposition of plant litter in preserved Caatinga vegetation, and the interference of climatic variability in the dynamics of these events. This research was developed in the Private Natural Heritage Reserve (RPPN), Tamanduá Farm, in the municipality of Santa Terezinha-PB, in a Caatinga area. Plant litter deposited in 20 twenty 1.0 m² litterfall traps of 1.0 m × 1.0 m was collected monthly (June/2014 to July/2015), covering the dry and rainy period of the region. The material was separated into leaves, branches + bark, reproductive material and miscellaneous material. Litter stock accumulated on the forest floor was quantified using a 0.5 m × 0.5 m metal frame to estimate decomposition rate of litter. We found that that leaves fraction obtained the highest deposition average in July 2014 with 395.80 kg ha-1 and the lowest in February/2015 with 9.5 kg ha-1. We concluded that that litter production obtained during the evaluation period was 2.2 mg ha-1, being the highest contribution of litter at the end of the rainy season of the region.

scholarly journals BIOGEOQUIMICA ESPAÇO-TEMPORAL DA LITEIRA EM AMBIENTE DE FLORESTA NATURAL NA AMAZÔNIA CENTRAL

Química Nova ◽  
2021 ◽  
Author(s):  
Ananda Rebêlo ◽  
Maria Monteiro ◽  
Sávio Ferreira ◽  
Eduardo Villamizar ◽  
Ézio Sargentini Junior ◽  
...  
Keyword(s):  
Chemical Elements ◽  
Icp Oes ◽  
Dry Period ◽  
Forest Environment ◽  
Reference Base ◽  
Reproductive Material ◽  
The Mean ◽  
One Year ◽  
Topographic Positions

SPACE-TEMPORAL BIOGEOCHEMISTRY OF THE LITTER IN A NATURAL FOREST ENVIRONMENT IN THE CENTRAL AMAZON. This research was aimed to evaluate the concentration of 15 chemical elements as a reference base for the quality of the litter in a forest in the central region of the Amazon. The sampling of the litter was performed monthly in two topographic positions for one year. For the quantification of the elements, digestion by nitroperchloric solution and reading by ICP OES were performed. The mean of macronutrient contents in the litter in decreasing order were Ca>K>Mg>Na. For micronutrients, the order was Al>Mn>Fe>Sr>Zn>Cu>Ba>Cr>Ni>Co>Cd for the Plateau and Al>Mn>Fe Sr>Zn>Cu>Ba>Cr>Ni>Cd>Co for the Baixio. Among the fractions, calcium had its highest concentration in the woody material, potassium in the reproductive material, while magnesium, sodium, and aluminum showed higher concentrations in the leaf fraction. Also, it was observed that the return of the elements to the soil by litter was greater in the dry period of the region, due to the greater fall in plant material. Calcium differed significantly within the same area, obtaining the highest concentrations compared to the other elements, while aluminum and zinc differed significantly between the areas studied, with a higher return for both elements on the Plateau.

Stateof-the-art of realization of the fertility potential in the women of late reproductive age

2016 ◽  
Vol 94 (1) ◽  
pp. 10-15
Author(s):  
Kristina S. Ermolenko ◽  
V. E. Radzinsky ◽  
S. I. Rapoport
Keyword(s):  
In Vitro Fertilization ◽  
Reproductive Technologies ◽  
Reproductive Age ◽  
Vitro Fertilization ◽  
Biological Potential ◽  
Reproductive Material ◽  
Seasonal Biorhythms

Biological potential of childbearing in the women of late reproductive age is limited by natural impairment and loss of fertility. Despite a considerable progress in clinical application of new diagnostic and reproductive technologies, the problem of infertility remains a most serious challenge. Women’s age is one of the main factors responsible for the outcome of in vitro fertilization. The low effectiveness of in vitro fertilization programs is attributed to discoordination in the hypothalamic-pituitary system, depletion of ovarian resources, and deterioration of quality of reproductive material. Bearing in mind the role of melatonin in synchronizing circadian and seasonal biorhythms and regulating physiological and pathological processes, it is natural to suggest its role in the enhancement of efficiency of in vitro fertilization programs for women of late reproductive age.

scholarly journals The effect of low-intensive coherent seed irradiation on germinant growth of Scots pine and sugar beet

2021 ◽  
Vol 67 (No. 9) ◽  
pp. 427-435
Author(s):  
Arthur Novikov ◽  
Igor Bartenev ◽  
Olga Podvigina ◽  
Olga Nechaeva ◽  
Denis Gavrin ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Scots Pine ◽  
Exposure Time ◽  
Laser System ◽  
Agricultural Plant ◽  
Seed Irradiation ◽  
Reproductive Material ◽  
Standard Laser ◽  
Positive Effect

The success of forest and agricultural plant establishment program mainly depends on the quality of reproductive material. The study intends to offer engineers and farm owners a solution for small-size seed improvement before sowing. The effect of low-intensity coherent light on the seeds of various crops is theoretically and empirically hypothesized. The seedlots of Scots pine (Pinus sylvestris L.) and sugar beet (Beta vulgaris L.) of Russian diploid hybrid RMS-127 were germinated in a controlled environment. The germinants were produced from six seed fractions, previously irradiated with 1.274 W·m<sup>–2</sup> at the 632.8 nm wavelength with 1, 2, 3, 5, 10, 15 min exposure to a standard laser system, plus untreated control. Pine germinants were measured on day 15, beetroot on day 10 after germination. An increase in exposure time reduced Scots pine germination energy and capacity, while for sugar beet the results were not conclusive. On the contrary, increasing the exposure time had a positive effect on both the height and biomass growth of both Scots pine and sugar beet germinants. The 10-min exposure time resulted in maximum values for sugar beet height and biomass and Scots pine height, while the 15-min exposure time produced maximum Scots pine biomass.

scholarly journals Performance of Scots Pine Seedlings from Seeds Graded by Colour

Forests ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1064 ◽  
Author(s):  
Arthur Novikov ◽  
Sergey Sokolov ◽  
Michael Drapalyuk ◽  
Vladimir Zelikov ◽  
Vladan Ivetić
Keyword(s):  
Growth Rates ◽  
Field Performance ◽  
Coat Colour ◽  
Seed Processing ◽  
Pine Seedlings ◽  
Visible Wavelength Range ◽  
Forest Reproductive Material ◽  
Reproductive Material ◽  
One Year

Research Highlights: One of the ways to improve the quality of a seedlot used in the forest nursery is the grading of seed by colour. Background and Objectives: The study is intended for forest’s engineers and owners because it offers an alternative solution for forest seeds improvement before sowing. The success of forest establishment program mainly depends on the quality of Forest Reproductive Material. At this time usual practices during the seed processing is seed grading on size. This causes a lot of controversy about the possible reduction of genetic diversity through directional selection. Materials and Methods: Aiming to study the effect of seed coat colour on seedling performance, a one-year old container seedlings of Pinus sylvestris L. were planted at the post-fire site. Seedlings were produced from three fractions, previously graded in the visible wavelength range on a standard optical separator, plus control obtained without separation by colour. Results: Seedlings from different seed fractions performed differently in the first growing season after planting on the field. Seedlings from light seed fraction grow better in height, but those from dark seed fraction resulted with the highest survival rate. Light-dark seeds, which constitute the largest group in the initial sample by absolute weight, resulted with seedlings of the lowest growth rates and survival. The good results showed by seedlings from the control, for both growth rates and survival, indicate the weak effect of seed colour grading on seedlings field performance, but also the need for the more comprehensive studies in the future.

scholarly journals MORPHOMETRIC ASSESSMENT OF GROWTH AND QUALITY OF SCOTS PINE PLUS TREES ON FOREST SEED PLANTATIONS

2020 ◽  
Vol 38 (4) ◽  
pp. 114-127
Author(s):  
V.F. Konovalov ◽  
◽  
E.R. Khanova ◽  
D.A. Rafikova ◽  
◽  
...  
Keyword(s):  
Scots Pine ◽  
Tree Species ◽  
Growth Patterns ◽  
Research Results ◽  
Family Forest ◽  
Pine Trees ◽  
Reproductive Material ◽  
Morphometric Assessment

The article presents research on the assessment of growth patterns, development and quality of Scots pine trees in relation to their breeding affiliation. Positive trees are identified that are the most promising for harvesting valuable reproductive material-seeds of improved breeding category and vegetative material-cuttings for creating clonal and family forest-seed plantations of Scots pine in the conditions of the dyurtyulinsky forestry. Based on the research results, recommendations are given for improving breeding activities with this tree species.

Immunoferritin localization of intracellular antigens in positively stained frozen sections

1978 ◽  
Vol 36 (2) ◽  
pp. 168-169
Author(s):  
K. T. Tokuyasu
Keyword(s):  
Surface Tension ◽  
Water Surface ◽  
Thin Layers ◽  
The Other ◽  
Positive Staining ◽  
Frozen Sections ◽  
The Past ◽  
Ultrathin Frozen Sections ◽  
Definition Of

During the past investigations of immunoferritin localization of intracellular antigens in ultrathin frozen sections, we found that the degree of negative staining required to delineate u1trastructural details was often too dense for the recognition of ferritin particles. The quality of positive staining of ultrathin frozen sections, on the other hand, has generally been far inferior to that attainable in conventional plastic embedded sections, particularly in the definition of membranes. As we discussed before, a main cause of this difficulty seemed to be the vulnerability of frozen sections to the damaging effects of air-water surface tension at the time of drying of the sections.Indeed, we found that the quality of positive staining is greatly improved when positively stained frozen sections are protected against the effects of surface tension by embedding them in thin layers of mechanically stable materials at the time of drying (unpublished).

Lithography below 100 nm

1983 ◽  
Vol 41 ◽  
pp. 96-99
Author(s):  
L. D. Jackel
Keyword(s):  
Spatial Distribution ◽  
Electron Beam ◽  
Electron Scattering ◽  
Electron Beam Lithography ◽  
Substrate Material ◽  
Local Electron ◽  
Electron Dose ◽  
Positive Resist ◽  
Exposure Process

Most production electron beam lithography systems can pattern minimum features a few tenths of a micron across. Linewidth in these systems is usually limited by the quality of the exposing beam and by electron scattering in the resist and substrate. By using a smaller spot along with exposure techniques that minimize scattering and its effects, laboratory e-beam lithography systems can now make features hundredths of a micron wide on standard substrate material. This talk will outline sane of these high- resolution e-beam lithography techniques.We first consider parameters of the exposure process that limit resolution in organic resists. For concreteness suppose that we have a “positive” resist in which exposing electrons break bonds in the resist molecules thus increasing the exposed resist's solubility in a developer. Ihe attainable resolution is obviously limited by the overall width of the exposing beam, but the spatial distribution of the beam intensity, the beam “profile” , also contributes to the resolution. Depending on the local electron dose, more or less resist bonds are broken resulting in slower or faster dissolution in the developer.

Convergent Beam Electron Diffraction

1978 ◽  
Vol 36 (3) ◽  
pp. 304-315
Author(s):  
G. Lehmpfuhl
Keyword(s):  
Electron Diffraction ◽  
Diffraction Pattern ◽  
Crystal Surface ◽  
Diffraction Spot ◽  
Crystal Thickness ◽  
Large Area ◽  
Electron Microscopic ◽  
Additional Information ◽  
Microscopic Investigations

Introduction In electron microscopic investigations of crystalline specimens the direct observation of the electron diffraction pattern gives additional information about the specimen. The quality of this information depends on the quality of the crystals or the crystal area contributing to the diffraction pattern. By selected area diffraction in a conventional electron microscope, specimen areas as small as 1 µ in diameter can be investigated. It is well known that crystal areas of that size which must be thin enough (in the order of 1000 Å) for electron microscopic investigations are normally somewhat distorted by bending, or they are not homogeneous. Furthermore, the crystal surface is not well defined over such a large area. These are facts which cause reduction of information in the diffraction pattern. The intensity of a diffraction spot, for example, depends on the crystal thickness. If the thickness is not uniform over the investigated area, one observes an averaged intensity, so that the intensity distribution in the diffraction pattern cannot be used for an analysis unless additional information is available.

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