scholarly journals Phytochemical compounds of Guibourtia ehie and their antioxidant, urease and α-glucosidase inhibitory activities

2022 ◽  
Author(s):  
Laurent Voufack Lefack Bongmo ◽  
Gervais Mouthé Happi ◽  
George Bellier Tabekoueng ◽  
Mehreen Lateef ◽  
Alain François Kamdem Waffo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Activity ◽  
Stem Bark ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Phytochemical Compounds ◽  
Inhibitory Activities

Thirteen compounds (<b>1</b>‒<b>13</b>) were isolated and identified during phytochemical analysis of the leaves and stem bark of <i>Guibourtia ehie</i> (A. Chev) J. Leonard. Spectroscopic and spectrometric methods and the comparison of their results with those given in the literature were used to ascertain their structures. Furthermore, the acetylation of 3,3′-di-<i>O</i>-methylellagic acid 4′-<i>O</i>-β-D-xylopyranoside (<b>2</b>) afforded a new derivative 3,3′-di-<i>O</i>-methylellagic acid 4′-<i>O</i>-β-D-(4,2′′,4′′-triacetyl)-xylopyranoside (<b>2a</b>). Extracts, fractions, and isolated compounds were assessed for their antioxidant, urease, and α-glucosidase inhibitory activities. Compound <b>1</b> demonstrated potent antioxidant activity in the DPPH with an IC<sub>50</sub> value of 36.4 ± 0.2 µM, while rhaponticin (<b>3</b>), 2,6-dimethoxybenzoquinone (<b>4</b>), and taraxerol (<b>6</b>) exhibited a strong α-glucosidase inhibitory activity with the IC<sub>50</sub> values of 35.5 ± 0.1, 25.5 ± 0.2 and 43.4 ± 0.3 µM, respectively. The present study enriches the chemistry of <i>Guiboutia ehie</i> and provides further evidence on its bioactive constituents, which might help in the development of hypoglycaemic drugs.

scholarly journals ARGINASE INHIBITORY ACTIVITY OF STEM BARK EXTRACTS OF CAESALPINIA TORTUOSA ROXB

2018 ◽  
Vol 10 (1) ◽  
pp. 130
Author(s):  
Amirah Najid ◽  
Berna Elya ◽  
Arikadia Noviani
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Phenolic Content ◽  
Colorimetric Method ◽  
Stem Bark ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Methanol Extracts ◽  
Methanolic Extracts ◽  
Ic50 Values

Objective: This study aimed to evaluate the arginase inhibitory activity of Caesalpinia tortuosa Roxb. stem bark extracts.Methods: C. tortuosa Roxb. stem bark extracts were obtained through reflux extraction using n-hexane, ethyl acetate, and methanol and theirinhibitory activity against arginase was measured using a microplate reader at 430 nm. Active extracts were subjected to phytochemical analysisand based on the qualitative phytochemical analysis, quantitative data regarding flavonoid and phenolic contents were obtained. The total flavonoidcontent of active extracts was determined using AlCl3 colorimetric method, and the phenolic content was determined using Folin–Ciocalteu method.Results: Ethyl acetate and methanol extracts of C. tortuosa Roxb. inhibited activity of arginase with IC50 values of 33.81 and 11.58 μg/mL,respectively, nor-NOHA acetate as standard drug inhibited arginase with IC50 values of I3.77 μg/mL. Both active extracts contained saponins,tannins, and flavonoids. Ethyl acetate and methanol extracts showed a total flavonoid content of 7.41 mgQE/g and 5.05 mgQE/g and totalphenolic content of 27.55 mgGE/g and 17.16 mgGE/g, respectively. Methanol extracts had a higher inhibitory activity than ethyl acetateextracts despite having flavonoid and phenolic content, thereby suggesting no correlation between arginase inhibitory activity and flavonoidor phenolic content.Conclusion: Ethyl acetate and methanolic extracts of C. tortuosa Roxb. stem barks containing flavonoids, tannins, and saponins displayed arginaseinhibitory activity, and no correlation was observed between arginase inhibitory activity and flavonoid and phenolic content.

EVALUATION OF ANTIOXIDANT ACTIVITY OF UNRIPE AEGLE MARMELOS CORR. FRUITS

2018 ◽  
pp. 4-7
Author(s):  
Gupta D ◽  
John PP ◽  
Kumar P ◽  
Jain J
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Aegle Marmelos ◽  
Indian Origin ◽  
Ic50 Value ◽  
Phytochemical Compounds ◽  
Free Radical Scavenging Assay

Aegle marmelos Corr. (Rutaceae), commonly known as Bael, is a tree of Indian origin, well known from ancient period andprescribed for various ailments in Ayurveda. Utilization of bael fruit in day-to-day life has a great nutritional, environmental as well ascommercial importance. Every part of Aegle marmelos including stem, bark, root, leaves, fruit and seeds at all stages of maturity possesmedicinal virtues and has been used in Ethno medicine to exploit its medicinal properties. Objective: This study was undertaken to examine theantioxidant activity of methanolic extract of Aegle marmelos unripe or half ripe fruits. Material and Methods: The antioxidant activity was doneby using DPPH free radical scavenging assay. The IC50 (The concentration of sample required to scavenge 50% of DPPH free radical) wascalculated by plotting graph between % inhibition vs concentration. The ascorbic acid was used as standard antioxidant. Result and Discussion:The IC50 value of extract and ascorbic acid was found to be 62.59μg/ml and 2.80μg/ml. The antioxidant activity found in Aegle marmelos maybe associated with their main phytochemical compounds like flavonoids, phenols and tannins. Conclusion: This activity supports that the fruitcan be used as natural antioxidant to treat free radical induced cellular damages and can also be used as adjuvant with other drugs to givesynergistic effects.

scholarly journals α-Amylase Inhibition, Antioxidant Activity and Phytochemical Analysis of Calotropis gigantea (L.) Dryand

2020 ◽  
Vol 10 (1) ◽  
pp. 77-81
Author(s):  
Rajendra Gyawali ◽  
Bijay Bhattarai ◽  
Susan Bajracharya ◽  
Surakshya Bhandari ◽  
Puja Bhetwal ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Calotropis Gigantea ◽  
Ic50 Value ◽  
Loaded Column ◽  
Chloroform Methanol

Introduction: Antioxidant and α-Amylase inhibitory activity of methanolic extract of Calotropis gigantea (L.) Dryand leaves were evaluated. Methods: The antioxidant activity was evaluated by DPPH assay. The extract was fractionated in Silica gel loaded column chromatography (CC). All fractions were evaluated for their purity by TLC. Out of 11 fractions from CC, one fraction was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Results: The antioxidant activity of methanolic extract was found satisfactory (IC50268.80 µg/ml) as compared with ascorbic acid (141.82 µg/ml). TLC of a fractions showed a compound at Rf value at 0.45 in toluene: chloroform: methanol with mobile phase ratio 7:2:1 respectively. Conclusions: Total 17 compounds were identified by GC-MS of ethyl acetate fraction and 5-hydroxyl methyl furfural was major furan compound (59.49%). α-Amylase inhibitory activity of the same fraction showed IC50 value of 0.94 mg/ ml. The Nepalese originated C. gigentea (L.) Dryand possesses antioxidant and α-Amylase inhibitory property.

scholarly journals PHYTOCHEMICAL SCREENING AND EVALUATION OF LEAF PARTS OF HYPOESTES PHYLLOSTACHYA ROSEA FOR IN VITRO ANTIOXIDANT ACTIVITIES

2020 ◽  
pp. 54-58
Author(s):  
JENSON JACOB ◽  
MEENU JOHN
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Standard Procedure ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Soxhlet Apparatus ◽  
Total Antioxidant ◽  
Phytochemical Compounds ◽  
Reducing Power Assay

Objective: The aim of this study was focussed on phytochemical analysis, in vitro antioxidant activities of different solvent extracts of Hypoestes phyllostachya leaf. Methods: The leaves were washed efficiently, shade dried and coarsely powdered. The powdered leaf material of Hypoestes phyllostachya was successively extracted with hexane, chloroform, methanol and water using Soxhlet apparatus. Preliminary phytochemical screenings for polyphenolics, proteins, phytosteroids, flavonoids, tannins, glycosides, alkaloids carbohydrates, polyphenolics, saponins were done by following standard procedure. In vitro antioxidant activities of various solvent extracts were assessed using DPPH, ABTS, Hydroxy radical, total antioxidant capacity and reducing power assay. Polyphenolics and flavonoids were also estimated. Results: The results showed that most of the phytochemical compounds such as carbohydrates, proteins, steroids, fixed oils and fatty acids, flavonoids, alkaloids, saponins, tannins, polyphenolics and glycosides present in the methanolic and aqueous extract of Hypoestes phyllostachya. The in vitro antioxidant activity of Hypoestes phyllostachya leaf clearly showed that it has better antioxidant activity, which was confirmed by different assays. Conclusion: From this work, it can be concluded that Hypoestes phyllostachya has the potential to be a good antioxidant but have only a lesser amount of flavonoids and polyphenolic contents. This was the first report on the pharmacological activity of the Hypoestes phyllostachya.

scholarly journals Antioxidant Potential of Pine Needles: A Systematic Study on the Essential Oils and Extracts of 46 Species of the Genus Pinus

Foods ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 142
Author(s):  
Aikaterini Koutsaviti ◽  
Samer Toutoungy ◽  
Rouba Saliba ◽  
Sofia Loupassaki ◽  
Olga Tzakou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Chromatographic Separations ◽  
Organic Extract ◽  
Luminol Chemiluminescence ◽  
Organic Extracts ◽  
Hydroethanolic Extract

The antioxidant activity of the essential oils, as well as of the organic and hydroethanolic extracts, of the fresh needles of 54 pine taxa was evaluated using the peroxy-oxalate and luminol chemiluminescence assays. Among all evaluated essential oils, P. canariensis and P. attenuata displayed the highest levels of activity. P. contorta var. murrayana, followed by P. nigra var. caramanica, exhibited the highest antioxidant capacity among the organic extracts, while the most active hydroethanolic extract was that of P. nigra subsp. nigra. Based on the overall levels of activity, the latter taxon was selected for phytochemical analysis targeting the isolation of the bioactive constituents. As such, the organic extract of P. nigra subsp. nigra was subjected to chromatographic separations to yield 11 secondary metabolites (1−11) that were evaluated for their antioxidant activity. Nonetheless, the isolated compounds were found to be less active than the crude extract, thus suggesting the potential role of synergism.

scholarly journals PHYTOCHEMICAL COMPOUNDS AND ANTIOXIDANT ACTIVITY OF YELLOW VELVETLEAF FRUIT (LIMNOCHARIS FLAVA) EXTRACT

2019 ◽  
pp. 55-57
Author(s):  
ACE BAEHAKI ◽  
SHANTI DWITA LESTARI ◽  
NORYATI SIREGAR
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Power Method ◽  
Activity Assessment ◽  
Sample Extraction ◽  
Phytochemical Compounds

Objective: The purpose of this research was to observe the content of phytochemical compound and antioxidant activity of yellow velvetleaf fruit (Limnocharis flava) extract. Methods: Research consisted of several stages, including sampling, sample preparation, sample extraction, calculation of yield extract, phytochemical analysis (flavonoids, steroids, alkaloids, saponins, and triterpenoids), and antioxidant activity assessment using 2’,2’-diphenyl-1-picrylhydrazyl and iron-reducing power method. Results: The n-hexane and methanol extract contained flavonoids, saponins, and triterpenoids, while flavonoids and triterpenoids were detected on ethyl acetate extract. The IC50 of yellow velvetleaf extract with n-hexane, ethyl acetate, and methanol was 3321.67 ppm, 1439.24 ppm, and 96.0 ppm, respectively. The methanol extract had the highest iron-reducing power with the average absorbance of 0.588 followed by the ethyl acetate extract with the average absorbance of 0.195 and n-hexane extract had lowest with the average absorbance of 0.171. Conclusion: The best solvent to extract the yellow velvetleaf and have highest antioxidant activity was methanol solvent.

scholarly journals Assessment of Anticholinesterase Activity ofGelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer’s Disease

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Arif Nisha Syad ◽  
Karutha Pandian Shunmugiah ◽  
Pandima Devi Kasi
Keyword(s):  
Spectrophotometric Method ◽  
Inhibitory Activity ◽  
Therapeutic Potential ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Gelidiella Acerosa ◽  
Cholinesterase Inhibitory Activity ◽  
Inhibitory Activities ◽  
Benzene Extract

The effect of various solvent extracts ofGelidiella acerosaon acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities was investigated. AChE and BuChE inhibitory activities were analyzed by spectrophotometric method. Phytochemical screening of the compounds present in the solvent extracts was done qualitatively. Characterization of the compounds present in the benzene extract ofG. acerosawas done by GC-MS analysis. The results showed that, at 487.80 μg/mL, benzene extract showed significant (P<0.05) inhibitory activity against both AChE and BuChE with the percentage of inhibition54.18±5.65% (IC50 = 434.61±26.53 μg/mL) and78.43±0% (IC50 = 163.01±85.35 μg/mL), respectively. The mode of inhibition exhibited by benzene extract against the AChE and BuChE was found to be competitive and uncompetitive type of inhibition, respectively. Preliminary phytochemical analysis coupled with GC-MS illustrates that the benzene extract possesses high amount of terpenoids, which could be the reason for potential cholinesterase inhibitory activity.

scholarly journals Phytochemical Analysis and Evaluation of Biological Activity of Lawsonia inermis Seeds Related to Alzheimer’s Disease

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Majid Balaei-Kahnamoei ◽  
Mina Saeedi ◽  
Arezoo Rastegari ◽  
Mohammad Reza Shams Ardekani ◽  
Tahmineh Akbarzadeh ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Oleanolic Acid ◽  
Inhibitory Activity ◽  
Phytochemical Analysis ◽  
Lawsonia Inermis ◽  
Reference Drug ◽  
Ic50 Values

Using Lawsonia inermis L. (henna) seeds has been frequently recommended for the improvement of memory in Iranian Traditional Medicine (ITM). In this respect, different fractions of the plant were prepared and evaluated for their in vitro biological assays related to Alzheimer’s disease (AD), including acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity as well as metal chelating ability and DPPH antioxidant activity. The dichloromethane and ethyl acetate fractions were able to inhibit the BChE selectively with IC50 values of 113.47 and 124.90 μg/mL, respectively, compared with donepezil as the reference drug (IC50 = 1.52 μg/mL). However, all fractions were inactive toward AChE. Phytochemical analysis of the dichloromethane fraction indicated the presence of β-sitosterol (1), 3-O-β-acetyloleanolic acid (2), 3-O-(Z)-coumaroyl oleanolic acid (3), betulinic acid (4), and oleanolic acid (5). The inhibitory activity of isolated compounds was also evaluated toward AChE and BChE. Among them, compounds 2 and 5 showed potent inhibitory activity toward BChE with IC50 values of 77.13 and 72.20 μM, respectively. However, all compounds were inactive toward AChE. Moreover, molecular docking study confirmed desired interactions between those compounds and the BChE active site. The ability of fractions and compounds to chelate biometals (Cu2+, Fe2+, and Zn2+) was also investigated. Finally, DPPH antioxidant assay revealed that the ethyl acetate (IC50 = 3.08 μg/mL) and methanol (IC50 = 3.64 μg/mL) fractions possessed excellent antioxidant activity in comparison to BHA as the positive control (IC50 = 3.79 μg/mL).

scholarly journals Phytochemical screening and biological activities of pakoba (Syzygium luzonense) stem bark ethanol extract

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
Mario Walean ◽  
ROSTINA MELPIN ◽  
MERVINA RONDONUWU ◽  
KINZIE FELICIANO PINONTOAN ◽  
Hendra Pratama Maliangkay ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Activity Index ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Optimum Dose ◽  
Phytochemical Analysis ◽  
Phytochemical Screening

Abstract. Walean M, Melpin R, Rondonuwu M, Pinontoan KF, Maliangkay HP, Astriani M. 2020. Phytochemical screening and biological activities of pakoba (Syzygium luzonense) stem bark ethanol extract. Biodiversitas 21: 2377-2382. Pakoba (Syzygium luzonense (Merr.) Merr) as an endemic and ethnomedical plant of North Sulawesi was investigated for its phytochemical constituents and biological activities. The presence of phytochemicals constituents, antibacterial, antioxidant activities, and antihyperglycemic of pakoba stem bark ethanolic extract (PSBEE) were also investigated. Pakoba stem bark was extracted using ethanol. The extracts were evaluated for antimicrobial activity using the Kirby Bauer method, whereas antioxidant activity was determined by using 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay. Alloxan-induced diabetic rats investigation was used to determine the antihyperglycemic activity. The phytochemical analysis revealed that tannins, alkaloids, and flavonoids were present in PSBEE. PSBEE has potent antioxidant activity with antioxidant activity index = 1.02. PSBEE significantly reduced the blood sugar of hyperglycemic rats with an optimum dose of 300 mg/kgbw. In this study, PSBEE has weak antibacterial activity against in Escherichia coli and Staphylococcus aureus.

scholarly journals Effects of Aqueous Stem Bark Extract of Stereospermum kunthianum on Carbon Tetrachloride - Induced Hepatotoxicity in Rats

2021 ◽  
pp. 44-52
Author(s):  
Mahmoud Suleiman Jada ◽  
Mohammed Aliyu Sulaiman ◽  
Muktar Abdulmalik
Keyword(s):  
Antioxidant Activity ◽  
Intraperitoneal Administration ◽  
Stem Bark ◽  
Bark Extract ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Antioxidant Power ◽  
Stem Bark Extract ◽  
Control Group Group

Aim: This study is aimed at evaluating the effects of the aqueous stem bark extract of Stereospermum kunthianum on CCl4-induced hepatotoxicity in rats. Methodology: Analysis of qualitative phytochemical components and antioxidant activity were carried out. Experimental rats were randomly divided into six groups of five rats each. Group 1: served as the normal control group. Group 2: was administered with CCl4 only at a dose of 3 ml/kg b.wt by single intraperitoneal administration. Group 3: served as the standard control group.   Group 4: was administered with 200 mg/kg b.wt of the aqueous stem bark extract + CCl4. Group 5: was administered with 400 mg/kg b.wt of the aqueous stem bark extract + CCl4. Group 6: was administered with 600 mg/kg b.wt of the aqueous stem bark extract+ CCl4. Results: The phytochemical analysis showed the presence of flavonoid, phenols, saponins, and terpenoid while tannins and alkaloids were absent. The antioxidant activity showed that the extract significantly (P<0.05) inhibits Ferric Reducing Antioxidant Power (FRAP) and Thiobarbituric Acid Reactive Substances (TBARS), giving high activity as the concentration of the extract increases. The elevated levels of ALT and AST coupled with Conjugated bilirubin, Total bilirubin, and total protein caused by CCl4 administration were all reduced significantly (P<0.05) by the extract in dose dependent manner. Conclusion: These findings demonstrated that stem bark extract of Stereospernmum kunthianum could be an alternative medication for liver injury.

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