scholarly journals Substrates and Temperatures in the Germination of Hibiscus sabdariffa L. Seeds

2018 ◽  
Vol 10 (12) ◽  
pp. 493
Author(s):  
J. J. A. Gomes ◽  
R. L. A. Bruno ◽  
P. A. F. R. de Melo ◽  
E. U. Alves ◽  
J. R. G. Araújo ◽  
...  
Keyword(s):  
Food Security ◽  
Dry Mass ◽  
Food Crops ◽  
Hibiscus Sabdariffa ◽  
Seedling Production ◽  
Germination Test ◽  
Paper Towel ◽  
Seedling Length ◽  
Germination Speed ◽  
Prevention Of Hypertension

Vegetable biodiversity in Brazil accounts for almost 67% of the plants on the planet, which are part of studies with focus on determinants of food security and access to adequate and healthy food, while preserving natural resources. Studies indicate that Hibiscus sabdariffa L. is one of the most studied unconventional food crops because of its nutraceutical quality as antioxidant and prevention of hypertension and cancer. Although relevant, substrate and temperature information for seedling production of this species are scarce. Thus, the aim of this study was to evaluate the best substrate and temperature for the vigor and germination test of H. sabdariffa seeds of the Talo Roxo and Rubra varieties. The experiment was laid out in a randomized complete design, in a 7 × 5 factorial arrangement, consisting of the combinations of seven substrates (paper towel, filter paper, vermiculite, between sand, on sand, plantmax® and compost) with five temperatures (20, 25, 30, 35 and 20-30 °C), with four replicates with 50 seeds each. The following variables were evaluated: germination, germination speed index, seedling length and dry mass. For germination and vigor tests of Hibiscus sabdariffa var. Talo Roxo seeds the paper roll substrate is recommended at temperatures of 25 or 35 °C and for var. Rubra seeds, plantmax® at 25 °C and plantmax® and vermiculite at temperatures of 25 or 20-30 °C, respectively.

Initial development of millet with use of biostimulant under different temperatures

2020 ◽  
Vol 13 (2) ◽  
pp. 20
Author(s):  
P. R. Diesel ◽  
V. N. Silva
Keyword(s):  
Thermal Stress ◽  
Low Temperature ◽  
Seed Treatment ◽  
Stress Condition ◽  
Dry Mass ◽  
Negative Effects ◽  
Germination Test ◽  
Initial Development ◽  
Germination Speed ◽  
Different Temperatures

Millet is an important summer grass used as forage grass due to good bromatological characteristics and easy adaptation to Brazilian environmental conditions. However, when sowing the crop early in the southern region, situations may occur where low temperatures affect germination and seedling development. Thus, the present work had the objective of evaluating the germination and initial development of millet using Biozyme TF® biostimulant as a way to attenuate the effects of different temperatures. The experimental design was completely randomized, in a factorial scheme 3 x 5 (temperatures x doses) with four replicates each. The temperatures used were 15 °C, 20 °C and 25 °C and the doses used were: 0 (control); 0.25; 0.5; 0.75 and 1 ml kg-1. After the treatment, the seeds were evaluated by: germination test, germination speed index, shoot and root length and seedling dry mass. The data obtained were submitted to analysis of variance with Sisvar® software and, when found significant, the regression analysis was performed. The germination of millet seeds is favored, under low temperature stress conditions, with seed treatment at the dose of 0.50 ml kg-1 of biostimulant. The biostimulant does not influence millet germination speed index. Under low temperature thermal stress the biostimulant minimally increases the length and dry mass accumulation of millet seedlings and attenuates the negative effects on root growth. The accumulation of dry mass of the roots decreased with increasing dose of the biostimulant under thermal stress condition.

scholarly journals Silicon alters early development of bean seedlings from seeds infected by Anthracnose

2021 ◽  
Vol 17 (3) ◽  
pp. 39-51
Author(s):  
Patrícia Migliorini ◽  
Cristina Rossetti ◽  
Andreia da Silva Almeida ◽  
Bruna Barreto Reis ◽  
Gustavo Fonseca Rodrigues ◽  
...  
Keyword(s):  
Early Development ◽  
Root Length ◽  
Aerial Part ◽  
Dry Matter ◽  
Dry Mass ◽  
Colletotrichum Lindemuthianum ◽  
Seed Coating ◽  
Germination Test ◽  
Initial Development ◽  
Seedling Length

The present study aimed to evaluate the effect of seed coating with Si in response to infection in a cultivar susceptible and resistant to Colletotrichum lindemuthianum, on the physiological and health potential of seeds and seedlings, during the initial development of bean culture. The experiment was carried out in a completely randomizeddesign, in a 2 × 2 × 2 factorial scheme, with four replications, where the factors were: cultivar (BRS Esplendor and IPR Tuiuiú), silicate application (with and without Agrosilício® coating) and inoculation (seeds inoculated or not with C. lindemuthianum). In the evaluation of the initial development of the culture, the following variables were measured: germination, first count of the germination test, emergence, emergence speed index (IVE), total seedling length, shoot and root length, total dry mass, aerial part dry matter, root dry matter and aerial part dry matter at 15 days after sowing (DAS). The incidence of C. lindemuthianumin beans was determined by the paper roll method. Covering the seeds with Si reduces the incidence and severity of C. lindemuthianumand provides greater growth of bean seedlings. The effect of responses potentiated by Si is more expressive in the cultivar of greater susceptibility.

scholarly journals Evaluation of Trichoderma spp. Isolates in Cocoa Seed Treatment and Seedling Production

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1964
Author(s):  
Willian Nogueira de Sousa ◽  
Nayane Fonseca Brito ◽  
Cristina Aledi Felsemburgh ◽  
Thiago Almeida Vieira ◽  
Denise Castro Lustosa
Keyword(s):  
Plant Growth ◽  
Seed Treatment ◽  
Plant Root ◽  
Soil Fungus ◽  
Dry Mass ◽  
Seedling Production ◽  
Trichoderma Spp ◽  
Germination Speed ◽  
Promote Plant Growth ◽  
Number Of Leaves

Isolates of Trichoderma spp., a soil fungus, has been used to control diseases and promote plant growth, reducing the use of chemicals in the production of seedlings of different plant species. We evaluated the effect of some Trichoderma spp. isolates on seed treatment and seedling production of Theobromacacao. Five isolates from the Amazon region were tested. In laboratory, the following variables were evaluated for seed treatments: germination, germination speed index, radicle and hypocotyl lengths, and fungi incidence. In nursery, the following forms of application were tested: via seeds; in the substrate at pre-planting; monthly in post-planting substrate, and also their combination. The following was evaluated: height, diameter, number of leaves, root length, leaf area, and shoot dry mass and root system. Inoculation with Trichoderma increased the length of the radicle and hypocotyl and showed no fungi in the seeds. In seedlings, some treatments increased height and plant root dry mass. The use of Trichoderma was beneficial for seeds and appeared favorable for T. cacao production.

scholarly journals Seed Technology of Myrcianthes pungens (Berg) Legr: An Approach to Biometry and Germination

2019 ◽  
Vol 11 (15) ◽  
pp. 144
Author(s):  
Adriana Maria Griebeler ◽  
Maristela Machado Araujo ◽  
Daniele Guarienti Rorato ◽  
Felipe Turchetto ◽  
Luciane Almeri Tabaldi ◽  
...  
Keyword(s):  
Laboratory Tests ◽  
Fine Sand ◽  
Germination Test ◽  
Seed Technology ◽  
Seedling Length ◽  
Hard Seeds ◽  
Germination Speed ◽  
Length Width ◽  
Digital Caliper ◽  
Seeds Germination

Myrcianthes pungens, native fruit with medicinal, ornamental and ecological potential, lacks information on biometry and technology for seed germination. Thus, the aim of this study was to characterize the fruits and seeds of M. pungens, determining the appropriate substrate for laboratory tests, as well as to evaluate the effect of different concentrations of gibberellic acid (GA3) on the germination of the species. In the biometry, the mensuration of the fruits and seeds was made with the aid of a digital caliper. In the laboratory, three substrates (between filter paper, between vermiculite and between sand) were used, with five concentrations of GA3 (0; 125; 250; 375 and 500 mg L-1) and the tests were conducted in a germination chamber. The percentage of germination, hard seeds, germination speed index and seedling length root and shoot were evaluated. The average fruit length and width were 17.38 and 16.05 mm, respectively. The fruits presented one or two seeds with 8.10; 9.56 and 6.49 mm in length, width and thickness. The germination test is more efficient between fine sand, and vermiculite may also be used. The wetting of the substrate with gibberellin in the concentration of 125 to 274 mg L-1 optimizes the percentage and speed of germination.

scholarly journals SUBSTRATES AND TEMPERATURES FOR THE GERMINATION OF SEEDS OF Senegalia tenuifolia (L.) BRITTON & ROSE

2016 ◽  
Vol 29 (1) ◽  
pp. 113-118 ◽  
Author(s):  
ALCIMONE MARIA SILVA ARAÚJO2 ◽  
LIZ CAROLINA DA SILVA LAGOS CORTES ASSIS2 ◽  
NARJARA WALESSA NOGUEIRA ◽  
RÔMULO MAGNO OLIVEIRA DE FREITAS ◽  
SALVADOR BARROS TORRES
Keyword(s):  
Significant Interaction ◽  
Dry Mass ◽  
Shoot Length ◽  
Forest Species ◽  
Germination Test ◽  
Tukey Test ◽  
Seed Analysis ◽  
Germination Speed ◽  
Two Factors ◽  
Different Temperatures

ABSTRACT: The Rules for Seed Analysis and the Instructions for Seed Analysis of Forest Species have no recommendations for conducting the S. tenuifolia germination test. Therefore, the aim of the present study was to evaluate different temperatures and substrates to perform the germination test of S. tenuifolia seeds. The experimental design was completely randomized in a factorial 4 × 3 design and consisted of four substrates (paper roll; on paper; on sand and on vermiculite) and three temperatures (25, 30 and 35 °C), with four replicates of 25 seeds. The percentage of normal seedlings, the germination speed index, the shoot length, root length and dry mass of seedlings were evaluated. The Tukey test was used at 5% probability. There was a significant interaction between the temperatures and substrates tested for all variables, indicating that there is at least one ideal combination of the two factors that can increase the germination of seeds. The germination and vigor of S. tenuifolia seeds are influenced by the temperature and by the substrate used in the germination test. The combination of the paper roll substrates with a temperature of 25 and 30 °C was suitable for the germination of S. tenuifolia seeds.

scholarly journals Application of vigor indexes to evaluate the cold tolerance in rice seeds germination conditioned in plant extract

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sheila Bigolin Teixeira ◽  
Stefânia Nunes Pires ◽  
Gabriele Espinel Ávila ◽  
Bruna Evelyn Paschoal Silva ◽  
Victoria Novo Schmitz ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Seed Priming ◽  
Dry Mass ◽  
Economic Losses ◽  
Tolerance Index ◽  
Promising Tool ◽  
Germination Speed ◽  
Cold Tolerant ◽  
Rice Seeds ◽  
Carrot Extract

AbstractRice is a crop that presents sensitivity to cold, especially in the germination phase, which leads to high economic losses. Alternative management forms are essential to increase tolerance to low temperatures, and seed priming represents a promising tool. The objective of this study was to investigate the priming effect of the aqueous extract of carrot roots on rice seeds to increase tolerance to low temperatures during germination. Seeds from cultivars BRS Querência (cold-susceptible) and Brilhante (cold-tolerant) were soaked for 24 h in concentrations of 0, 25, 50, and 100% carrot extract, sown on germitest paper and conditioned in BOD for 21 days at 15 °C. As a control, the seeds soaked in water were also germinated at 25 °C. They were evaluated for germination, first germination count, and germination speed index to calculate the stress indices: tolerance index, susceptibility index, and harmonic mean. They were also evaluated for the length and dry mass of shoot and root. The results showed that the rice seeds conditioning in carrot extract effectively reduces the damage caused by cold, significantly increasing the germination speed and the percentage of final germination and the growth evaluations, more expressive at 100% concentration. The stress indexes are efficient in estimating the tolerance of the cultivars and the effect of the different conditions in low-temperature conditions, highlighting the superiority of the Brilhante cultivar.

scholarly journals Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

2014 ◽  
Vol 36 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Daiana Karoline Kaiser ◽  
Laura Cristiane Nascimento de Freitas ◽  
Rubia Priscila Biron ◽  
Simone Cristina Simonato ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Viability ◽  
Germination Test ◽  
Preparation Methods ◽  
Eugenia Uniflora ◽  
Paper Towel ◽  
Plastic Bags ◽  
Longitudinal Cutting ◽  
Direct Immersion ◽  
Tetrazolium Test

The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.

scholarly journals Seedling imaging analysis and traditional tests to assess okra seed vigor

2013 ◽  
Vol 35 (4) ◽  
pp. 443-448 ◽  
Author(s):  
Ana Lúcia Pereira Kikuti ◽  
Júlio Marcos-Filho
Keyword(s):  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Control Programs ◽  
Seed Industry ◽  
Accelerated Aging Test ◽  
Vigor Index ◽  
Seedling Length ◽  
Germination Speed

Seed vigor testing is an important component of quality control programs adopted by seed industry. The software Seed Vigor Imaging System (SVIS) has been successfully used for seed vigor assessment in different species. The objective of this research was to verify the SVIS efficiency to assess okra seed vigor in comparison to other vigor tests used for this species. Five seed lots of 'Clemson Americano' and four of 'Santa Cruz' were submitted to germination (speed and percentage), cold germination (speed and percentage), traditional and saturated salt accelerated aging (41 ºC/72 and 96 h) and seedling emergence tests during 12 months storage. Vigor index, uniformity of growth and seedling length were determined by the software Seed Vigor Imaging System (SVIS). Results showed that the vigor index and seedling length determined by the SVIS analyses are efficient to determine okra seed vigor as well as the accelerated aging test (41 ºC/72 h).

scholarly journals Campomanesia adamantium (Cambess.) O. Berg seed desiccation: influence on vigor and nucleic acids

2015 ◽  
Vol 87 (4) ◽  
pp. 2217-2228 ◽  
Author(s):  
DAIANE M. DRESCH ◽  
TATHIANA E. MASETTO ◽  
SILVANA P.Q. SCALON
Keyword(s):  
Moisture Content ◽  
Silica Gel ◽  
Primary Root ◽  
Dry Mass ◽  
Rna Integrity ◽  
Laboratory Conditions ◽  
Dna And Rna ◽  
Seed Desiccation ◽  
Seedling Length ◽  
Activated Silica Gel

The aim of this study was to evaluate the sensitivity of Campomanesia adamantium seeds to desiccation by drying in activated silica gel (fast) and under laboratory conditions (slow). To assess the sensitivity of the seeds to desiccation, we used drying with silica gel and drying under laboratory conditions (25 °C), in order to obtain seeds with moisture content of 45, 35, 30, 25, 20, 15, 10 and 5%. The physiological potential of the seeds after desiccation was evaluated by measuring primary root protrusion, percentage of normal seedlings, germination seed index, seedling length, total seedling dry mass, electrical conductivity and DNA and RNA integrities. The C. adamantium seeds were sensitive to desiccation and to a reduction in moisture content to 21.1% or less by desiccation using silica gel, and to 17.2% or less by desiccation under laboratory conditions; impairment of the physiological potential of the seeds was observed at these low moisture content levels. The integrity of the seed genomic DNA was not affected after drying seeds in the two methods. However, drying in silica gel to 4.5% moisture content and drying under laboratory conditions to 5.4% moisture content resulted in the loss of seed RNA integrity.

scholarly journals Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction

2020 ◽  
Vol 50 (5) ◽  
Author(s):  
Gabriel Streck Bortolin ◽  
Alfredo José Salinas-Arcienega ◽  
Yutcelia Carolina Galviz-Fajardo ◽  
Luciano do Amarante ◽  
Carlos Eduardo da Silva Pedroso ◽  
...  
Keyword(s):  
Seed Germination ◽  
Physiological Responses ◽  
Dry Mass ◽  
Germination Percentage ◽  
Early Growth ◽  
Paspalum Notatum ◽  
Water Restriction ◽  
Germination Test ◽  
Growth Activity ◽  
Higher Temperature

ABSTRACT: The objective of the following research was to investigate the physiological responses of two bahiagrass genotypes (Paspalum notatum Flügge) submitted to water restriction, induced by polyethylene glycol (PEG6000), during seed germination. Seeds of cv. Pensacola (diploid) and Bagual ecotype (tetraploid) were placed on paper moistened with osmotic solutions at potentials of 0.0; -0.1; -0.2 and -0.3 MPa. Composed of two subsamples of 100 seeds and four experimental units per treatment, the germination test was conducted in a germination chamber under alternating temperatures of 30 °C (8 hours) and 20 °C (16 hours), with presence of light during the higher temperature. Final germination percentage and germination speed index of the seeds, as well as early growth, activity and expression of antioxidant enzymes of seedlings were evaluated. The water restriction induced by PEG6000 reduced the percentage of germination in Pensacola (from 74% to 59%) and Bagual (34% to 10%). For the latter, the evaluation of seedlings showed higher dry mass in relation to cv. Pensacola (1.7 times higher in root and 1.6 times in shoot). In response to moderate water restriction, the genotypes showed increases in root growth (1.7 times higher in -0.1 compared to 0.0 MPa), both in expression and enzymatic activity (superoxide dismutase and catalase).

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