Application of vigor indexes to evaluate the cold tolerance in rice seeds germination conditioned in plant extract

AbstractRice is a crop that presents sensitivity to cold, especially in the germination phase, which leads to high economic losses. Alternative management forms are essential to increase tolerance to low temperatures, and seed priming represents a promising tool. The objective of this study was to investigate the priming effect of the aqueous extract of carrot roots on rice seeds to increase tolerance to low temperatures during germination. Seeds from cultivars BRS Querência (cold-susceptible) and Brilhante (cold-tolerant) were soaked for 24 h in concentrations of 0, 25, 50, and 100% carrot extract, sown on germitest paper and conditioned in BOD for 21 days at 15 °C. As a control, the seeds soaked in water were also germinated at 25 °C. They were evaluated for germination, first germination count, and germination speed index to calculate the stress indices: tolerance index, susceptibility index, and harmonic mean. They were also evaluated for the length and dry mass of shoot and root. The results showed that the rice seeds conditioning in carrot extract effectively reduces the damage caused by cold, significantly increasing the germination speed and the percentage of final germination and the growth evaluations, more expressive at 100% concentration. The stress indexes are efficient in estimating the tolerance of the cultivars and the effect of the different conditions in low-temperature conditions, highlighting the superiority of the Brilhante cultivar.

Download Full-text

Osmotic priming of rice seeds subjected to low temperatures

Journal of Seed Science ◽

10.1590/s2317-15372013000200013 ◽

2013 ◽

Vol 35 (2) ◽

pp. 231-235 ◽

Cited By ~ 1

Author(s):

Fernanda Reolon Tonel ◽

Patrícia Marini ◽

Juliana de Magalhães Bandeira ◽

Ana Carolina Silveira da Silva ◽

Norton Victor Sampaio ◽

...

Keyword(s):

Seed Germination ◽

Low Temperatures ◽

Seedling Emergence ◽

Control Treatment ◽

Distilled Water ◽

Randomized Design ◽

Significant Difference ◽

Germination Speed ◽

Completely Randomized Design ◽

Rice Seeds

Aiming at verifying effects of priming on germination and emergence of seeds subjected to low temperatures, rice seeds (cv. IRGA 418) were primed into a KNO3 solution during 24, 48, and 72 h, at 20 °C; and into distilled water, for 24 h (control). Germination (G), first count of germination (FCG), and germination speed index (GSI) were the parameters assessed under temperatures of 17 °C, 20 °C and 25 °C. Besides, field emergence (FE), and emergence speed index (ESI) were also assessed under field conditions. A completely randomized design was used with treatments arranged in 4 x 3 factorial (treatments x temperatures) in all experiments. None statistically significant difference was found for seed germination within the 24 h imbibition period, under any of temperatures assessed; although after 48 and 72 h, at 17 °C, this variable has decreased. When seeds were imbibed in KNO3, during 48 and 72 h, the FCG and GSI were decreased, as compared to 24 h imbibition period, and for control treatment, at 17 °C and 25 ºC. After 24 h imbibition, the FE has not differed statistically from control treatment; as occurred for periods of 24 and 48 h imbibition for the ESI parameter. Priming in KNO3 does not cause beneficial effect on seed germination or on seedling emergence of rice.

Download Full-text

Germination and vigor of Caesalpinia pulcherrima (L.) Sw. seeds under different light and temperature conditions

Ciência Rural ◽

10.1590/0103-8478cr20170741 ◽

2018 ◽

Vol 48 (8) ◽

Author(s):

Magnólia Martins Alves ◽

Edna Ursulino Alves ◽

Luciana Rodrigues de Araújo ◽

Maria de Lourdes dos Santos Lima ◽

Marina Matias Ursulino

Keyword(s):

Experimental Design ◽

Central America ◽

White Light ◽

Dry Mass ◽

Light Regime ◽

Shoot Length ◽

Light Regimes ◽

Seed Analysis ◽

Germination Speed ◽

Living Fence

ABSTRACT: Caesalpinia pulcherrima is a Fabaceae family species from Central America, which has a small size and is widely used as a living fence in the urban afforestation for streets, parks, and gardens. Based on this importance, the aim of this research was to study the influence of light and temperature on the germination of C. pulcherrima seeds. The study was carried out at the Laboratory of Seed Analysis of the Center of Agricultural Sciences of the Universidade Federal da Paraíba, Areia, PB, Brazil. The experimental design was completely randomized, comprising a 3x5 (three temperatures and five light regimes) factorial scheme with four replicates containing 25 seeds each. Germination and vigor of seeds were evaluated under temperatures of 25°C and 30ºC constant and 20-30ºC alternated in different light regimes: white, green, red-distant, red, and absence of light. Variables analyzed were: germination, first germination count, germination speed index, root length, shoot length, and the ratio of dry mass of root/shoot of the seedlings. Seeds of C. pulcherrima germinate in the presence and absence of light, being a neutral photoblastic. Recommendation to test the vigor of these seeds is the temperature of 30°C in the white light regime.

Download Full-text

Germination and seedling characteristics of drought-stressed corn seed as influenced by seed priming with potassium nano-chelate and sulfate fertilizers

Acta agriculturae Slovenica ◽

10.14720/aas.2016.107.1.12 ◽

2016 ◽

Vol 107 (1) ◽

pp. 113 ◽

Cited By ~ 1

Author(s):

Maryam ZAHEDIFAR ◽

Sadegh ZOHRABI

Keyword(s):

Drought Stress ◽

Seed Priming ◽

Dry Mass ◽

Plant Tolerance ◽

Corn Seed ◽

Seedling Traits ◽

Corn Seedling ◽

Seminal Roots ◽

Mean Germination Time ◽

Seedling Characteristics

Effect of seed-priming with potassium (K) sources (K-nano-chelate, KNC, and sulfate (0, 2 and 4 %)) under drought stress (DS) conditions (0, -0.3, -0.6, -0.9, -1.2 and -1.5 MPa water potential) on the corn seedling traits was studied. Drought stress decreased the germination indices and seedling vigor. The highest germination, seminal root fresh and dry mass (RFM and RDM) was obtained in KNC primed seeds at -0.3 MPa DS. Mean germination time increased under DS conditions mainly in non-primed seeds. Increasing DS to -1.2 MPa led to decrease in RFM and RDM. Influence of DS on the fresh mass of shoots was more severe than on seminal roots. The highest shoots and seminal roots length was observed in 4 % KNC without any DS. Proper priming can be suggested to increase the plant tolerance under DS.

Download Full-text

Leaf growth and K+/Na+ ratio as an indication of the salt tolerance of three sorghum cultivars grown under salinity stress and IAA treatment

Acta Agronomica Hungarica ◽

10.1556/aagr.52.2004.3.10 ◽

2004 ◽

Vol 52 (3) ◽

pp. 287-296 ◽

Cited By ~ 16

Author(s):

M. M. Azooz ◽

M. A. Shaddad ◽

A. A. Abdel-Latef

Keyword(s):

Salt Tolerance ◽

Water Content ◽

Leaf Area ◽

Salinity Stress ◽

Relative Water Content ◽

Leaf Growth ◽

Dry Mass ◽

Inhibitory Effect ◽

Tolerance Index ◽

Relative Water

The salt tolerance of three sorghum (Sorghum bicolor L.) cultivars (Dorado, Hagen Shandawil and Giza 113) and their responses to shoot spraying with 25 ppm IAA were studied. Salinity stress induced substantial differences between the three sorghum cultivars in the leaf area, dry mass, relative water content and tolerance index of the leaves. Dorado and Hagen Shandawil tolerated salinity up to 88 and 44 mM NaCl, respectively, but above this level, and at all salinity levels in Giza 113, a significant reduction in these parameters was recorded. The rate of reduction was lower in Dorado than in Hagen Shandawil and Giza 113, allowing the sequence Dorado ? Hagen Shandawil ? Giza 113 to be established for the tolerance of these cultivars to salinity. The differences in the tolerance of the sorghum cultivars were associated with large differences in K+ rather than in Na+, which was found to be similar in the whole plant. The youngest leaf was able to maintain a higher K+ content than the oldest leaf. Consequently the K+/Na+ ratios were higher in the most salt-tolerant cultivar Dorado than in the other sorghum cultivars, and in the youngest than in the oldest leaf. In conformity with this mechanism, the stimulatory effect of the exogenous application of IAA was mostly associated with a higher K+/Na+ ratio. Shoot spraying with IAA partially alleviated the inhibitory effect of salinity on leaf growth and on the K+ and Ca2+ contents, especially at low and moderate levels of salinity, while it markedly retarded the accumulation of Na+ in the different organs of sorghum cultivars. Abbreviations: LA: Leaf area, DM: Dry mass, I Indole acetic acid, RWC: Relative water content,TI: Tolerance index

Download Full-text

Nutrient homeostasis, metabolism of reserves, and seedling vigor as affected by seed priming in coarse rice

Canadian Journal of Botany ◽

10.1139/b06-088 ◽

2006 ◽

Vol 84 (8) ◽

pp. 1196-1202 ◽

Cited By ~ 56

Author(s):

M. Farooq ◽

S.M.A. Basra ◽

M. Khalid ◽

R. Tabassum ◽

T. Mahmood

Keyword(s):

Reducing Sugars ◽

Amylase Activity ◽

Nitrogen Concentration ◽

Ion Homeostasis ◽

Seed Priming ◽

Dry Mass ◽

Seedling Vigor ◽

Control Seed ◽

Positive Correlations ◽

Secondary Roots

The influence of seed priming on germination, seedling vigor, ion homeostasis, and starch metabolism in coarse rice was studied. Priming treatments included pregermination (a traditional soaking method being used for rice nursery preparation), hydropriming for 48 h, osmohardening with KCl or CaCl2 (ψs –1.25 MPa solution) for 24 h, ascorbate priming (10 mg·L–1) for 48 h, and hardening for 24 h. Compared with controls, all priming treatments (except pregermination) reduced the time to start germination, improved the rate of germination and synchronization, and the length of shoot and root, seedling fresh and dry mass, number of secondary roots, the concentration of reducing sugars, and α-amylase activity, although the extent of these changes was different in seeds subjected to different treatments. These seed treatments resulted in higher germination that might be due to overcoming dormancy. Osmohardening with KCl was more effective than CaCl2 for these parameters. Nitrogen concentration remained unaffected in seedlings; however, Ca2+ concentrations in both seeds and seedlings were greater in seeds osmohardenerd with CaCl2 than with all other treatments, including the control. Seed priming enhanced K+ concentration in both seeds and seedlings, leading to improved α-amylase activity. There were positive correlations between seed K+ concentration and amylase activity, and the concentration of reducing sugars with amylase activity, seedling dry mass, or number of secondary roots. Osmohardening with KCl performed better than all other treatments including control. Priming improved the K+ balance that activates α-amylase, a basis for seed invigoration.

Download Full-text

EFFECT OF SEEDS DRESSINGON THE INITIAL ESTABLISHMENT OF IRRIGATED RICE IN DIFFERENT TEMPERATURES AND SOWING PERIODS

Revista Científica Rural ◽

10.30945/rcr-v23i1.3242 ◽

2021 ◽

Vol 23 (1) ◽

pp. 94-109

Author(s):

Thaís D Avila Rosa ◽

Diogo Balbé Helgueira ◽

Marlon Ouriques Bastiani ◽

Ivan Ricardo Carvalho ◽

Mauro Mesko Rosa ◽

...

Keyword(s):

Low Temperatures ◽

Science Department ◽

Irrigated Rice ◽

Rice Plants ◽

Different Temperatures ◽

Rice Seeds ◽

Sowing Season

The study aimed to evaluate the effects of seeds dressing in the initial establishment of irrigated rice and in the tolerance to herbicides when submitted to low temperatures. The study comprehended two experiments, where the first stage was realized at the Laboratory of Seeds and Technology of the Plants Science Department of the Federal University of Pelotas. Experiment 1: Effect of different seed dressings under the physiologic potential of irrigated rice seeds and experiment 2: Effect of the seeds dressing in the initial establishment of irrigated rice in different sowing seasons in field. The treatment with dietholate and the combining of dietholate + fipronil + carboxina + tiram, negatively influenced the germination and the vigor tests in both temperatures, decreasing the physiologic performance of rice seeds in lab. When taken to field, the seeds dressing did not influence the analyzed factors, being dependent of the herbicide applying and the sowing season. Besides presenting phytotoxicity, the rice plants presented detoxification power from the herbicide, being possible to analyze this fact, since there was higher number of tillers, resulting in higher number of panicles resulting in higher productivities.

Download Full-text

WILD MEDICS FROM DIFFERENT ORIGINAL HABITATS CAN BE USED AS FORAGE LEGUMES IN SALT AFFECTED SOIL

Journal CleanWAS ◽

10.26480/jcleanwas.02.2020.47.55 ◽

2020 ◽

Vol 4 (2) ◽

pp. 47-55

Author(s):

Nadia Mohamed El-Shafey ◽

Emad Al-Sherif

Keyword(s):

Developmental Stages ◽

Initial Step ◽

Germination Percentage ◽

Wild Relatives ◽

Energetic Cost ◽

Tolerance Index ◽

Ion Leakage ◽

Natural Habitats ◽

Mineral Contents ◽

Germination Speed

Legumes are a key player in sustainable agriculture. They are a potential tool as forage for reclamation of saline soils. However, still there is a need to balance between tolerance of the forage during different developmental stages and its productivity. The present work aimed to study salinity tolerance of four wild Medicago species, as an initial step to select new species that can be grown in salt-affected soils or used as wild relatives to improve alfalfa. Seeds of M. polymorpha, M. intertexta, M. truncatula and M. lupulina, collected from different natural habitats, as well as alfalfa were germinated under different salinity levels to evaluate germination percentage and germination speed. Generally, seeds of M. truncatula collected from desert habitat showed the highest mean germination percentage followed by alfaalfa, while seeds of M. intertexta collected from salt-affected habitat exhibited the highest mean germination speed, followed by M. lupulina. Under severe salt stress, M. intertexta exhibited the highest aerial biomass index, followed by M. truncatula and M. lupulina, while M. polymorpha and alfalfa came as inferiors. Mineral contents and ion leakage of the studied species were determined and discussed. M. intertexta, M. truncatula and M. lupulina, collected from stressful habitats, tended to maintain osmotic and ionic homeostasis by relying on accumulation of the less energetic cost ions (Na+) in roots and sugars and K+ in shoots scoring the highest aerial biomass and tolerance index, orderly. Therefore, the results recommend cultivating these species in salt-affected lands or using them as wild relatives to improve alfalfa.

Download Full-text

Ameliorative Effect of Seed Priming on Germination, Vigour Index and Tolerance Index against Short Term Moisture Stress in Maize (Zea mays L.)

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-5351 ◽

2020 ◽

Author(s):

V. Singh ◽

M. Sharma ◽

H. Upadhyay ◽

A. Siddique

Keyword(s):

Polyethylene Glycol ◽

Seed Priming ◽

Moisture Stress ◽

Magnesium Nitrate ◽

Tolerance Index ◽

Short Term ◽

Seed Vigour ◽

Peg 6000 ◽

Vigour Index ◽

Polyethylene Glycol 6000

An experiment was carried out to evaluate the effect of seed priming with magnesium nitrate salt against the moisture stress condition induced by Polyethylene glycol-6000 on seed germination, vigour index and tolerance index in root and shoot. Polyethylene glycol-6000 was used to create short term moisture stress under laboratory conditions while the seed priming was carried out by using magnesium nitrate. The percent germination, seed vigour index and tolerance index were started to decline from T1 to T4 (i.e. set first 1.5 and 4.5 % of PEG-6000 solution + none primed seed) while the results of second set (i.e. PEG-6000 + primed seed from T5 to T8) were also following the same decreasing trend along with improvement in every parameters. The germination was recorded highest 100 percent in T5 (i.e. 1.5% PEG-6000+ primed seed) followed by T6, T1 and T7 (T6 and T7 is 2.5% and 3.5 % PEG-6000+ primed seed) while the control set recorded 98.7% at final date of observations i.e. 240 Hrs. vigour index and tolerance index was also decreasing in both the set of treatments but PEG-600 + primed seed showed better results as compared to the treatments PEG-6000 + none primed. Tolerance index percent was recorded maximum in root as compare to shoot at 120 hrs. of intervals while the reverse result was recorded at 240 hrs. of intervals.

Download Full-text

Bean seed performance under different temperatures

Journal of Biotechnology and Biodiversity ◽

10.20873/jbb.uft.cemaf.v8n1.groth ◽

2020 ◽

Vol 8 (1) ◽

pp. 012-016

Author(s):

Mártin Zanchett Groth ◽

Rodrigo Ferraz Ramos ◽

Cristiano Bellé ◽

Débora Leitzke Betemps ◽

Nariane De Andrade ◽

...

Keyword(s):

Phenotypic Plasticity ◽

Local Adaptation ◽

Temperature Increase ◽

Biomass Accumulation ◽

Dry Mass ◽

Southern Brazil ◽

Bean Seed ◽

Dry Biomass ◽

Germination Speed ◽

Different Temperatures

The study evaluated the germination performance of bean seeds (BRS Expedito) from different regions of southern Brazil submitted to different germination temperatures (20, 25 and 30º C). Were evaluated to germination (GER), first germination count (PCG), germination speed index (IVG), shoot dry mass (MSPA) and root dry mass (MSR). The seeds from São Mateus (PR) presented the highest average values for the variables GER, PCG and IVG in the three temperatures tested. The temperature increase (20 to 30 ºC) significantly influenced the dry biomass accumulation in the shoots and seedlings roots. The results indicate the existence of a phenotypic plasticity acquired by the cultivar due to local adaptation of the ecotypes.

Download Full-text

Initial development of millet with use of biostimulant under different temperatures

Scientific Electronic Archives ◽

10.36560/1312020818 ◽

2020 ◽

Vol 13 (2) ◽

pp. 20

Author(s):

P. R. Diesel ◽

V. N. Silva

Keyword(s):

Thermal Stress ◽

Low Temperature ◽

Seed Treatment ◽

Stress Condition ◽

Dry Mass ◽

Negative Effects ◽

Germination Test ◽

Initial Development ◽

Germination Speed ◽

Different Temperatures

Millet is an important summer grass used as forage grass due to good bromatological characteristics and easy adaptation to Brazilian environmental conditions. However, when sowing the crop early in the southern region, situations may occur where low temperatures affect germination and seedling development. Thus, the present work had the objective of evaluating the germination and initial development of millet using Biozyme TF® biostimulant as a way to attenuate the effects of different temperatures. The experimental design was completely randomized, in a factorial scheme 3 x 5 (temperatures x doses) with four replicates each. The temperatures used were 15 °C, 20 °C and 25 °C and the doses used were: 0 (control); 0.25; 0.5; 0.75 and 1 ml kg-1. After the treatment, the seeds were evaluated by: germination test, germination speed index, shoot and root length and seedling dry mass. The data obtained were submitted to analysis of variance with Sisvar® software and, when found significant, the regression analysis was performed. The germination of millet seeds is favored, under low temperature stress conditions, with seed treatment at the dose of 0.50 ml kg-1 of biostimulant. The biostimulant does not influence millet germination speed index. Under low temperature thermal stress the biostimulant minimally increases the length and dry mass accumulation of millet seedlings and attenuates the negative effects on root growth. The accumulation of dry mass of the roots decreased with increasing dose of the biostimulant under thermal stress condition.

Download Full-text