scholarly journals Differentiation of accessions from the national lentil collection according to the level of drought resistance on a various concentrations PEG-6000 solution

2021 ◽  
Vol 29 ◽  
pp. 68-73
Author(s):  
N. Vus ◽  
A. O. Vasylenko ◽  
L. M. Shevchenko

Aim. assessment  of  accessions from the lentil national collectionon the level of drought resistance on a PEG-6000 solution. Methods. The germination was carried out in growth plates on a filter paper bed at t = 21ºC for five days. A working solution of PEG-6000 with concentrations of 15.0% and 19.5% was used. The control was germinated in distilled water. The length of the shoot and root was measured and the level of depression of growth processes was calculated. Results. When germinating on a PEG-6000 solution with 19.5% concentration, the overwhelming number of accessions had depression indicators for shoots above 85% and above 60% for roots, and therefore it was not possible to isolate samples tolerant to the action of PEG-6000. When germinating on a 15%  solution of PEG-6000, a wider range of variation was noted: depression in feature of “root length” ranged from 0% to 100%, “shoot length” - from 50% to 100%. The calculated Spearman's rank correlation coefficient confirms that shoots, in comparison with roots, are more sensitive to the action of osmotic in both variants of the experiment. Conclusions. The concentration of PEG-6000 was established for the selection of sources and the ranking of samples by drought resistance. Sources of drought resistance were identified from the studied sample – accessions subsp. microsperma: 8-MIL M 5 (UD0600007, France), UD0600028 (Ethiopia), Giza 9 (UD0600065, Egypt) and the accession-standard of drought resistance is Gornostepnaya (UD0600131, Armenia) subsp. microsperma. Keywords: lentil, PEG-6000, concentration, sources of drought resistance.

Author(s):  
A. I. Zheltikov ◽  
T. V. Konovalova ◽  
O. I. Sebezhko ◽  
V. V. Ilyin ◽  
P. N. Palchikov ◽  
...  

The characteristic of bulls-producers of red breeds of OAO Barnaulskoe breeding enterprise on the quality of sperm and its resistance to cryopreservation are presented. The breeding enterprise has examined samples of water, soil, feed for the content of heavy metals. In the studied samples, no excess of the LOC was found. The research results were processed on a PC using population statistics methods. Angler bulls produced the largest amount of ejaculates unsuitable for cryopreservation. It is 20.6%. According to this indicator, they surpassed the producers of red Danish and red steppe breeds by 4.1-7.8%. The animals of the latter breed showed the smallest amount (1.7%) of ejaculates rejected after defrost. In Angler bulls, this indicator was 5.95%, in red Danish bulls it was 2.95%, which is 3.52 and 1.75 times higher than in red steppe breeders (P <0.01-0.001). Significant differences were established between individual bulls of the red steppe breed in the number of ejaculates rejected before cryopreservation (2.84-22.29%) and after defrost (0-7.09%). The differences between breeds in the frequency of rejected ejaculates before freezing and after cryopreservation indicate a certain role of heredity in the resistance of animals for these indicators. Spearman’s rank correlation coefficient between these traits was 0.312. It indicates the possibility of preliminary assessment and selection of bulls according to the first indicator. But the final assessment of the bull-producers can be made by the frequency of rejected ejaculates after thawing. We propose to consider the issue of additional accounting in breeding programs for the resistance of the seed of bulls-producers to cryopreservation.


2021 ◽  
Vol 16 ◽  
pp. 404-421
Author(s):  
Eslam Mohammed Abdelkader ◽  
Abobakr Al-Sakkaf ◽  
Ghasan Alfalah

Material selection is a very entangled and decisive stage in the design and development of products. There are large numbers of on hand and newly developed materials available in the market. In addition, inability to select the correct materials adversely affects the reputation and profitability of the company. Thus, designers need to study and trace the performance of available materials with appropriate functionalities. Thus, this research aims at establishing an efficient and systematic platform for the optimum selection of materials while accommodating the designated conflicting performance requirements. The developed model encompasses designing a hybrid decision support system in an attempt to circumvent the shortcomings of single multi-criteria decision making-based (MCDM) models. First, the objective relative importance weights of attributes are interpreted capitalizing on Shannon entropy algorithm. Then, an integrated model that encompasses the utilization of six different types of multi-criteria decision making algorithms is designed to create a reliable selection of material alternatives. The utilized MCDM algorithms comprise weighted product method (WPM), simple additive weighting (SAW), additive ratio assessment (ARAS), new combinative distance-based assessment (CODAS), complex proportional assessment (COPRAS) and technique for order of preference by similarity to ideal solution (TOPSIS). Afterwards, COPELAND algorithm is exploited to generate a consensus and distinct ranking of material alternatives. Eventually, Spearman’s rank correlation analysis is used to evaluate the rankings obtained from the MCDM algorithms. Five numerical examples in diverse fields of material selection are tackled to examine the features and efficiency of the developed integrated model. Results illustrated that the developed model was able to solve the five material selection problems efficiently. On the other hand, no individual MCDM algorithm was able to solve all the assigned material selection problems. For instance, CODAS and TOPSIS only succeeded in solving one and two material selection problems, respectively. It was also inferred that notable differences and perturbations are encountered between the rankings of MCDM algorithms in the first, third, fourth and fifth numerical examples, which necessitates the implementation of COPELAND algorithm. It was also revealed that the highest correlation lied between COPRAS and WPM with an average Spearman’s rank correlation coefficient of 92.67%. On the other hand, the correlation between TOPSIS and CODAS attained the lowest rank with an average Spearman’s rank correlation coefficient of 18.95%. Results also demonstrated that COPRAS accomplished the highest Spearman’s rank correlation coefficient with 59.54%. Hence, it is the most efficient MCDM algorithm among the five algorithms which can serve as a reference for solving material selection problems. It can be also deduced that CODAS and TOPSIS are not advised to be implemented in solving similar material selection problems.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Hui Jiang ◽  
Peian Lou ◽  
Xiaoluo Chen ◽  
Chenguang Wu ◽  
Shihe Shao

Abstract Background Type 2 diabetes mellitus (T2DM) is mainly affected by genetic and environmental factors; however, the correlation of long noncoding RNAs (lncRNAs) with T2DM remains largely unknown. Methods Microarray analysis was performed to identify the differentially expressed lncRNAs and messenger RNAs (mRNAs) in patients with T2DM and healthy controls, and the expression of two candidate lncRNAs (lnc-HIST1H2AG-6 and lnc-AIM1-3) were further validated using quantitative real-time polymerase chain reaction (qRT-PCR). Spearman’s rank correlation coefficient was used to measure the degree of association between the two candidate lncRNAs and differentially expressed mRNAs. Furthermore, the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway and GO (Gene Ontology) enrichment analysis were used to reveal the biological functions of the two candidate lncRNAs. Additionally, multivariate logistic regression analysis and receiver operating characteristic (ROC) curve analysis were performed. Results The microarray analysis revealed that there were 55 lncRNAs and 36 mRNAs differentially expressed in patients with T2DM compared with healthy controls. Notably, lnc-HIST1H2AG-6 was significantly upregulated and lnc-AIM1-3 was significantly downregulated in patients with T2DM, which was validated in a large-scale qRT-PCR examination (90 controls and 100 patients with T2DM). Spearman’s rank correlation coefficient revealed that both lncRNAs were correlated with 36 differentially expressed mRNAs. Furthermore, functional enrichment (KEGG and GO) analysis demonstrated that the two lncRNA-related mRNAs might be involved in multiple biological functions, including cell programmed death, negative regulation of insulin receptor signal, and starch and sucrose metabolism. Multivariate logistic regression analysis revealed that lnc-HIST1H2AG-6 and lnc-AIM1-3 were significantly correlated with T2DM (OR = 5.791 and 0.071, respectively, both P = 0.000). Furthermore, the ROC curve showed that the expression of lnc-HIST1H2AG-6 and lnc-AIM1-3 might be used to differentiate patients with T2DM from healthy controls (area under the ROC curve = 0.664 and 0.769, respectively). Conclusion The profiles of lncRNA and mRNA were significantly changed in patients with T2DM. The expression levels of lnc-HIST1H2AG-6 and lnc-AIM1-3 genes were significantly correlated with some features of T2DM, which may be used to distinguish patients with T2DM from healthy controls and may serve as potential novel biomarkers for diagnosis in the future.


Diagnostics ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 769
Author(s):  
Yuko Hara ◽  
Kenichi Goda ◽  
Shinichi Hirooka ◽  
Takehiro Mitsuishi ◽  
Masahiro Ikegami ◽  
...  

We previously reported that superficial non-ampullary duodenal tumors (SNADETs) commonly had a whitish mucosal surface, named milk-white mucosa (MWM). The aim of this study was to evaluate the association of MWM with epithelial intracellular lipid droplets (immunohistochemically stained by adipose differentiation-related protein (ADRP)) and histological tumor grades. We reviewed endoscopic images and the histopathology of SNADETs resected en bloc endoscopically. We analyzed the correlation between the positive rates of endoscopic MWM in preoperative endoscopy and resected specimens, and ADRP-positive rates in the resected specimens. Associations between the MWM-positive rates and tumor grades, high-grade intraepithelial neoplasia (HGIN)/intramucosal carcinoma (IC), and low-grade intraepithelial neoplasia (LGIN) were analyzed. All the 92 SNADETs analyzed were <20 mm and histologically classified into 39 HGIN/IC and 53 LGIN. Spearman’s rank correlation coefficient showed a significant correlation between MWM-positive and ADRP-positive rates (p < 0.001). MWM-positive rates were significantly lower in the HGIN/IC than in the LGIN in preoperative endoscopy (p < 0.001) and resected specimens (p = 0.02). Our results suggest that endoscopic MWM is closely associated with epithelial intracellular lipid droplets and that the MWM-positive rate may be a predictor of histological grade in small SNADETs.


2012 ◽  
Vol 16 (02) ◽  
pp. 209-216 ◽  
Author(s):  
Luana Berwig ◽  
Ana da Silva ◽  
Eliane Corrêa ◽  
Eliane Serpa ◽  
Rodrigo Ritzel

Summary Introduction: Hyperplasia of the pharyngeal tonsil is one of the main causes of mouth breathing, and accurate diagnosis of this alteration is important for proper therapeutic planning. Therefore, studies have been conducted in order to provide information regarding the procedures that can be used for the diagnosis of pharyngeal obstruction. Objective: To verify the correlation between nasopharyngoscopy and cephalometric examinations in the diagnosis of pharyngeal tonsil hyperplasia. Method: This was a cross-sectional, clinical, experimental, and quantitative study. Fifty-five children took part in this study, 30 girls and 25 boys, aged between 7 and 11 years. The children underwent nasofibropharyngoscopic and cephalometric evaluation to determine the grade of nasopharyngeal obstruction. The Spearman's rank correlation coefficient at the 5% significance level was used to verify the correlation between these exams. Results: In the nasopharyngoscopy evaluation, most children showed grade 2 and 3 hyperplasia of the pharyngeal tonsil, which was followed by grade 1. In the cephalometry assessment, most children showed grade 1 hyperplasia of the pharyngeal tonsil, which was followed by grade 2. A statistically significant regular positive correlation was observed between the exams. Conclusion: It was concluded that the evaluation of the pharyngeal tonsil hyperplasia could be carried out by fiber optic nasopharyngoscopy and cephalometry, as these examinations were regularly correlated. However, it was found that cephalometry tended to underestimate the size of the pharyngeal tonsil relative to nasopharyngoscopy.


2018 ◽  
Vol 23 (3) ◽  
Author(s):  
Urszula Kaczmarek ◽  
Wojciech Kowalczyk ◽  
Dariusz Gozdowski ◽  
Dorota Olczak-Kowalczyk

Introduction. The use of age- and risk-adjusted caries prevention requires up-to-date knowledge on the cariostatic effects of fluoride, as well as the methods and safety of fluoride prophylaxis. Aim. The aim of the study was to assess dentists’ knowledge of the safety and mechanism of anticaries effects of fluoride. Material and methods. An anonymous questionnaire was conducted among 212 dentists participating in dental training. The questions included in the questionnaire related to knowledge about water fluoridation, fluoride cariostatic mechanisms and the safety of fluoride prophylaxis. The chi-square test and the Spearman’s rank correlation coefficient were used for statistical analysis. A significance level of 0.05 was used. Results. Incorrect knowledge about water fluoridation was demonstrated by 26.9% of respondents in the country and 16.0% of respondents in Europe. Most respondents (80.7%) reported higher efficacy of exogenous vs endogenous anticaries approaches, and more than half of respondents (59.0%) considered the processes of remineralisation and demineralisation as the most important anticaries activity. Almost all respondents (95.7%) agreed that the use of fluoride for caries prevention is controversial, mainly due to its dose-dependent therapeutic or toxic effects (85.4%). According to about 20% of respondents, cariostatic fluoride doses have adverse effects on the general health, mainly in the form of bone fragility (10.4%). Conclusions. Lack of correct knowledge about the dominant cariostatic effects of fluoride may result in the choice of an inappropriate preventive method or avoiding local application of fluoride preparations for fear of adverse systemic effects, which may in turn lead to increased caries prevalence in a given population.


2018 ◽  
Vol 30 (4) ◽  
pp. 419-428
Author(s):  
Danwen Bao ◽  
Xiaoling Zhang ◽  
Jiayu Gu

To scientifically and accurately evaluate the status of the development of green airports in China, evaluation methods of green, ecological airports are established in this paper. To address the shortcomings in subjective and objective weighting methods, we propose a combination weighting method based on Spearman’s rank correlation coefficient and evaluation grades based on interval approximation. At the same time, by taking into account resource conservation, environmental friendliness, operation efficiency, and people-oriented service, we propose an evaluation index system and an interval number for each index. Lastly, the theory is applied to five large airports in different regions of China. Analysis of the evaluation results shows that Shanghai Pudong International Airport (PVG) and Guangzhou Baiyun International Airport (CAN) have the highest scores for the resource conservation and environmental friendliness indexes, thus indicating that the development of a green ecological airport is closely related to its passenger transportation scale and economic strength. All considered airports showed the need for upgrading public  service facilities and constructing intelligent equipment. The method proposed in this paper is reasonable  and reliable; therefore, it can provide guidance for the evaluation and construction of green, ecological  airports.


2021 ◽  
Vol 7 (4) ◽  
pp. 58-68
Author(s):  
T. Chernykh ◽  
V. Mulyk ◽  
K. Mulyk ◽  
А. Skaliy ◽  
А. Ostrowski ◽  
...  

Purpose: to determine the relationship between the performance of basic elements and indicators of static and dynamic balance in the training process of young acrobats 6-7 years. Material and methods. The study involved 16 young acrobats at the stage of initial training (age 6-7 years). All parents of the participants gave written consent for the participation of children in the study. The study involved testing the technique of performing basic elements of sports acrobatics and assessing the static and dynamic balance of young athletes. The relationship between (swallow, shoulder blade, forward squat, wheel (sideways overturning), bridges) and static and dynamic equilibrium tests was determined. Spearman's rank correlation coefficient method was used as a method of statistical analysis. Results. It is established that the largest relationship between the performance of basic elements and static balance in the basic exercises of young acrobats have: Romberg's test with the elements "Swallow", "Stand on the shoulders", "Bridge"; Biryuk test with elements "Swallow", "Wheel", "Bridge"; balance "Swallow" with elements "Swallow", "Wheel"; static equilibrium test with the elements "Swallow", "Rack on the shoulders", "Rolling forward with a squat", "Wheel". Reliable values ​​of the correlation coefficient were established between the tests of dynamic balance and the basic elements of sports acrobatics in young athletes 6-7 years, namely: "Swallow", "Flip forward with a squat", "Wheel" and "Bridge". The analysis of special tests of static and dynamic balance in mastering the basic elements of acrobatics at the first stage of long-term training makes it possible to use them to determine the level of coordination capabilities of young athletes and further improve the use of acrobatic exercises. Conclusions. A reliable relationship between the performance of basic exercises and indicators of static and dynamic balance in young acrobats at the initial stage of training. It is shown that the level of static and dynamic balance is of great importance for the assimilation and improvement of basic elements of technology by young acrobats 6-7 years. Static and dynamic balance tests can be used to individually build training programs and young acrobats. It is shown that sports acrobatics is a significant means of developing the balance of children 6-7 years.


Author(s):  
Satyanand Sathi ◽  
Richa Tiwari ◽  
Savita Verma ◽  
Anil Kumar Garg ◽  
Virendra Singh Saini ◽  
...  

Recent literature has reported that radiological features of coronavirus disease (COVID-19) patients are influenced by computed tomography. This study aimed to assess the characteristic chest X-ray features of COVID-19 and correlate them with clinical outcomes of patients. This retrospective study included 120 COVID-19 patients. Baseline chest X-rays and serial chest X-rays were reviewed. A severity index in the form of maximum radiological assessment of lung edema (RALE) score was calculated for each lung, and scores of both the lungs were summed to obtain a final score. The mean ± standard deviation (SD) and frequency (%) were determined, and an unpaired t test, Spearman’s rank correlation coefficient, and logistic regression analyses were performed for statistical analyses. Among 120 COVID-19 patients, 74 (61.67%) and 46 (38.33%) were males and females, respectively; 64 patients (53.33%) had ground-glass opacities (GGO), 55 (45.83%) had consolidation, and 38 (31.67%) had reticular-nodular opacities, with lower zone distribution (50%) and peripheral distribution (41.67%). Baseline chest X-ray showed a sensitivity of 63.3% in diagnosing typical findings of SARS-CoV-2 pneumonia. The maximum RALE score was 2.13 ± 1.9 in hospitalized patients and 0.57 ± 0.77 in discharged patients ( p value <0.0001). Spearman’s rank correlation coefficient between maximum RALE score and clinical outcome parameters was as follows: age, 0.721 ( p value <0.00001); >10 days of hospital stay, 0.5478 ( p value <0.05); ≤10 days of hospital stay, 0.5384 ( p value <0.0001); discharged patients, 0.5433 ( p value <0.0001); and death, 0.6182 ( p value = 0.0568). The logistic regression analysis revealed that maximum RALE scores (0.0932 [0.024–0.367]), (10.730 [2.727–42.206]), (1.258 [0.990–1.598]), and (0.794 [0.625–1.009]) predicted discharge, death, >10 days of hospital stay, and ≤10 days of hospital stay, respectively. The study findings suggested that the RALE score can quantify the extent of COVID-19 and can predict the prognosis of patients.


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